СОСУД ДЛЯ ОПТИЧЕСКОГО УСТРОЙСТВА ДЛЯ АНАЛИЗА КРОВИ, АНАЛИЗАТОР, ОСНАЩЕННЫЙ ТАКИМ СОСУДОМ

Изобретение относится к проточному сосуду, адаптированному к оптическому устройству для подсчета и/или дифференциации лейкоцитов в автоматическом устройстве, анализирующем кровь. Проточный сосуд для оптического устройства для подсчета и/или дифференциации лейкоцитов в автоматическом аналитическом устройстве для анализа крови содержит корпус сосуда. Также устройство содержит инжектор, который расположен приблизительно соосно с корпусом сосуда вдоль оси инжектирования для инжектирования потока пробы. При этом корпус и инжектор расположены соосно вдоль оси инжектирования, которая перпендикулярна оптической оси. Кроме того, устройство содержит средство для формирования потока-оболочки вокруг потока пробы. При этом поток пробы инжектируется под давлением относительно потока-оболочки. Причем поток-оболочка играет пассивную роль, поскольку не определяет ширину потока пробы, растягивая его. Устройство также содержит зону анализа, которая расположена ниже по потоку от инжектора. При этом вдоль оптической ...

ЛАЗЕРНЫЙ ДАТЧИК ДЛЯ ОБНАРУЖЕНИЯ НЕСКОЛЬКИХ ПАРАМЕТРОВ

Изобретение относится к области оптико-электронного приборостроения и касается модуля лазерного датчика. Модуль содержит лазер, детектор, электропривод и оптическое устройство. Модуль лазерного датчика выполнен с возможностью управлять расстоянием между модулем лазерного датчика и областью фокусировки и обеспечивать два режима модуля лазерного датчика. Причем в первом и втором режимах область фокусировки находится на разном расстоянии. Детектор выполнен с возможностью обнаруживать сигнал интерференции самосмешения оптической волны в лазерном резонаторе. Сигнал интерференции самосмешения в первом режиме используется для обнаружения движения объекта для управления посредством жестов для устройства с человеко-машинным интерфейсом. Сигнал интерференции самосмешения во втором режиме используется для определения плотности или размера частиц. Технический результат заключается в обеспечении возможности одновременного определения двух различных физических параметров посредством интерференции самосмешения ...

СИСТЕМЫ И СПОСОБЫ ДЛЯ ДИАГНОСТИКИ СИСТЕМЫ ПОДАЧИ ТЕКУЧИХ СРЕД И ОПРЕДЕЛЕНИЯ НАСТРОЕК ОБРАБОТКИ ДАННЫХ ДЛЯ ПРОТОЧНОГО ЦИТОМЕТРА

ЛАЗЕРНЫЙ ДАТЧИК ДЛЯ ОБНАРУЖЕНИЯ НЕСКОЛЬКИХ ПАРАМЕТРОВ

Устройство для оптического определения размеров и числа взвешенных частиц

Изобретение относится к оптическому анализу биологических материалов, например протеина, клеток, вирусов и т.д. Цель изобретения - упрощение конструкции и улучшение эксплуатационных характеристик устройства за счет исключения необходимости юстировки пучков. Для этого два луча с различной длиной волны пропускают через общий волоконно-оптический световод с общей фокусирующей линзой, что позволяет решить проблему их совмещения. 2 з.п. ф-лы, 1 ил.

Gas or fluid particle analysis using light-scattering - has laser source with opto-acoustic filter and transient recorder for particle identification

The apparatus for the analysis of particles in fluids or air uses the light-scattering principle. The light source used is a gas-ionisation laser. The secondary radiation emitted by the particles illuminated by the laser beam is passed through an optical system to a continuously-variable acoustic-optical filter. The output of the filter is connected to a photo-multiplier. The electronically scanned output is in a narrow band of wavelengths and each time-point in the scanning cycle corresponds to a definite wavelength of the secondary emission. The photo-multiplier signal at a certain time-point is proportional to the intensity of the secondary emission at that wavelength. A transient recorder stores this information for the electronic processor for identification of the particles.

Partikelmessvorrichtung und Verfahren zum Betrieb derselben

Eine Partikelmessvorrichtung (1) hat einen optischen Partikelsensor (2, 3, 5, 6). Mittels des optischen Partikelsensors (2, 3, 5, 6) ist eine Partikelmassenkonzentration in einem Aerosolvolumen erfassbar. Des Weiteren hat die Partikelmessvorrichtung (1) eine Messkammer (7), in der das hinsichtlich der Partikelmassenkonzentration mittels des optischen Partikelsensors (2, 3, 5, 6) zu untersuchende Aerosolvolumen aufnehmbar ist, und eine Fördereinrichtung (11), mittels der das Aerosol in die Messkammer (7) einleitbar ist.Um zu verhindern, dass die Partikelmessvorrichtung (1) im Betrieb durch Schmutz- bzw. Partikelablagerungen beeinträchtigt wird, wird vorgeschlagen, dass die Fördermenge der Fördereinrichtung (11) der Partikelmessvorrichtung (1) einstellbar ist.

VERFAHREN ZUR AUTOMATISCHEN DIFFERENTIALZAEHLUNG DER LEUKOZYTEN IN EINER BLUTPROBE

Detection of a fluid-borne particle using optical scattering

Detecting the size and concentration of fluid-borne particles using optical light scattering. A flow of particles 10 passes through laser beam 12 in a scattering chamber. A single reflector or refractor means such as an elliptical or spherical mirror 14 is positioned such that its primary focus 16 lies on the axis of the laser beam 12 in an optically defined particle sensing zone. Scattered light from the particles in the optically defined particle sensing zone is reflected by mirror 14 to photo-detector 24 placed at the secondary focus 20 of the mirror 14. The photo-detector 24 comprises two photosensitive detection areas 26, 28. Means are provided for deriving data from the radiation detected by the first photosensitive detection area 26 and second photosensitive detection area 28 of the detection means. Therefore the elliptical mirror 14 and photo-detector 24 provide a method of optically defining a sensing zone within the illuminating laser beam.

Sensing methods and apparatus

A method of selectively detecting the presence of an analyte comprises providing at least one waveguide having a porous core material, absorbing an analyte sample into said porous core such that said analyte sample is held within pores of said core, wave-guiding radiation along said at least one waveguide to an output to provide output radiation, measuring one or more spectral features of said output radiation due to absorption or scattering of said wave-guided radiation by said absorbed analyte sample, and selectively identifying the presence of a target analyte in said analyte sample from said one or more spectral features. Spectral features may be measured for multiple different waveguide core regions having different physical/chemical properties modified to provide additional selectivity to the target analyte(s), and these measurements combined to identify the target analyte.

High-content imaging of microfluidic devices

The present invention is related to high-content microscopy imaging of microfluidic cell culture systems. A method of high-content microfluidic device microscopy is contemplated, along with related statistical analysis and microfluidic device adaptors.

Particle detection system and components thereof

Fluid-borne particle detector

Mobile microscopy system for air quality monitoring

A lens-free microscope for monitoring air quality includes a housing that contains a vacuum pump configured to draw air into an impaction nozzle. The impaction nozzle has an output located adjacent to an optically transparent substrate for collecting particles. One or more illumination sources are disposed in the housing and are configured to illuminate the collected particles on the optically transparent substrate. An image sensor is located adjacent to the optically transparent substrate, wherein the image sensor collects particle diffraction patterns or holographic images cast upon the image sensor. At least one processor is disposed in the housing and controls the vacuum pump and the one or more illumination sources. Image files are transferred to a separate computing device for image processing using machine learning to identify particles and perform data analysis to output particle images, particle size, particle density, and/or particle type data.

Advanced biophysical and biochemical cellular monitoring and quantification using laser force cytology

The present invention is directed to intelligent algorithms, methodologies and computer-implemented methodologies for biophysical and biochemical cellular monitoring and quantification enabling enhanced performance and objective analysis of advanced infectivity assays including neutralization assays and adventitious agent testing using fluidic and optical force-based measurements.

Method for the cytometric analysis of cell samples

A method for the cytometric analysis of cell samples, wherein the microscope is capable of operating in a transmis­sion mode and/or in a fluorescence mode. The cell sample has a fluorescence marker. During a continuous movement of the cell samples, a first image of a sub-region of the cell sample is recorded in transmission or fluorescence mode; and a second image of the cell sample is recorded in fluorescence mode. The two images are associated with each other. Positions and/or con­tours M of cells or cell components of the cell samples are detected in the transmission image. The in­tensities of the fluorescence image are then analysed as a function of these positions and/or contours M. Alternatively, positions and/or contours M are extracted from any one of these two images, and the remaining image is analysed as a function of these positions and/or contours.

Photothermal detection system

Advanced biophysical and biochemical cellular monitoring and quantification using laser force cytology

Systems and methods for automated single cell cytological classification in flow

Filter unit for an opitcal particle counter

A filter unit 1 for demountable fitting at the inlet of an optical particle counter (OPC), wherein the filter comprises a central conduit 3 for supplying a central sample flow 11 to the counter, and a filtering portion for supplying a filtered sheath flow 13 surrounding the central sample flow. The filter unit may comprise a sheath flow conduit 5, which may be tapered and extend further in the direction of intended gas flow than the central conduit, for supply of the filtered sheath flow to the counter. In normal operation, the filtering portion can comprise a filter 9 which may be annular, located around the central conduit, and which offers low flow impedance. The filter may be housed within a housing 7, comprising a base and separable cap portion to allow replacement of the filter. The cap is preferably impermeable and the base portion allows ingress of atmospheric gas, to provide the filtered sheath. An optical particle counter comprising the filter unit is disclosed wherein a fan may ...

DEVICE FOR THE DETECTION OF PARTICLES IN A FLUID

DEVICE FOR THE DETECTION OF PARTICLES IN A FLUID

DEVICE FOR THE DETECTION OF PARTICLES IN A FLUID

Microfluidic flow analyzer for pathological detection and method thereof

Microfluidic flow analyzer for pathological detection and method thereof

Microfluidic flow analyzer for pathological detection and method thereof

SYSTEM AND PROCEDURE FOR THE EPIDETEKTIONSKOHÄRENZ ANTI- STOKES RAMAN STREUMIKROSKOPIE ONES

PROCEDURE FOR THE INVESTIGATION OF GEGESTÄNDEN OF MACROMOLECULAR SIZE

Kondensationspartikelzähler mit einer Sättigungseinheit und einer nachgeschalteten Kondensationseinheit

A condensation particle counter comprising a saturation unit (1) and a downstream condensation unit (2), through which at least one channel (5) for an aerosol flow passes between an inlet (3) and an outlet (4) that leads to a counting unit (16). The saturation unit (1) and the condensation unit (2) comprise a shell sleeve (12) having an inner shell wall (21) that is penetrated by a core (6), the core wall (20) and the inner shell wall (21) delimiting a channel (5) formed therebetween.

Vorrichtung und Verfahren zur Zählung und/oder Messung von Partikeln in einem Fluidstrom

Die Erfindung betrifft eine Vorrichtung und ein Verfahren zur Zählung und/oder Messung von Partikeln (1) in einem Fluidstrom (3). Die Vorrichtung weist eine Lichtquelle (4) zur Durchleuchtung des Fluidstroms (3) in einem Bereich eines Messkanals (2), der vom Fluidstrom (3) durchflossen wird, und einen Lichtsensor (6) mit einer Vielzahl an Sensorelementen (7), die in dem von der Lichtquelle (4) ausgestrahlten Lichtbündel und an der der Lichtquelle (4) gegenüberliegenden Seiten des Messkanals (2) angeordnet sind, auf. Es ist eine Auswerteeinheit (12) vorgesehen, die eine Vielzahl an zeitlich aufeinanderfolgenden Sensormessungen (13) zu einem Sensorbild (9) zusammensetzt und zur Messung und/oder Zählung der Partikel (1) Interferenzmuster (10) in dem Sensorbild (9), die von den Partikeln (1) verursacht werden, auswertet.

PORTABLE PARTICLE ANALYZING DEVICE.

DURCHFLUSSZYTOMETRIE.

METHODS, SYSTEMS, AND APPARATUS FOR PERFORMING FLOW CYTOMETRY

Abstract An apparatus for detecting an analyte in a sample includes an illumination source for generating electromagnetic energy to illuminate the sample at an interrogation region, a concave collector element having an optical axis, and a focal point, the interrogation region being coincident with the focal point of the concave collector element, a closed flow cell having a flow path defined between a sample inlet and a sample outlet, the flow path passing through the interrogation region and a sorting region disposed downstream of the interrogation region. The portion of the flow path passing through the interrogation region is coaxially aligned with the optical axis of the concave collector element. The apparatus further includes the sample comprising or suspected of comprising the analyte and flowing in the flow path, wherein the analyte generates a detectable signal in response to illumination. The apparatus also includes a detector for detecting the detectable signal.

Method and apparatus for analysis of particles in a liquid sample

AUTOMATIC MIXING AND DILUTION METHODS AND APPARATUS FOR ONLINE CHARACTERIZATION OF EQUILIBRIUM AND NON-EQUILIBRIUM PROPERTIES OF SOLUTIONS CONTAINING POLYMERS AND/OR COLLOIDS

Optical detection of particle characteristics

A method of detecting particles in an air flow is described. The method includes receiving a signal indicative of light intensity scattered from the air flow at a plurality of wavelengths and processing the signal indicative of the intensity of received light at each of the wavelengths and a corresponding wavelength dependent parameter to generate an output signal indicative of at least one characteristic of particles in the air flow. A particle detection system is also described.

Method and apparatus for selectively targeting specific cells within a cell population

Method and apparatus for selectively targeting specific cells within a cell population

DISPERSION ANALYSIS OF AEROSOLS

CLASSIFYING BIOLOGICAL CELLS BY FLUORESCENT RADIATION USING SPLIT LASER BEAM

HIGH NUMERICAL APERTURE FLOW CYTOMETER AND METHOD OF USING SAME

A high numerical aperture flow cytometer having a laser input (20) which emits a beam of light oriented substantially orthogonally to the flow of blood cells through a flow cell (18). A portion of the beam impinging upon the cells is scattered at a substantially right angle to the beam of laser input and is received by a right angle scatter light detector (22). A second portion of the beam impinging upon the cells in the flow cell is scattered at a much lower angle than 90~ and is collected on two distinct photo detectors, that represent 'forward scatter low' which has an angle of from about 1~ to about 3~ and 'forward scatter high' which has an angle of from about 9~ to about 12~ from the original beam from laser input. A third photo detector is placed between the two forward scatter detectors and measures axial light loss.

OPTICAL DETECTION OF PARTICLE CHARACTERISTICS

A method of detecting particles in an air flow is described. The method includes receiving a signal indicative of light intensity scattered from the air flow at a plurality of wavelengths and processing the signal indicative of the intensity of received light at each of the wavelengths and a corresponding wavelength dependent parameter to generate an output signal indicative of at least one characteristic of particles in the air flow. A particle detection system is also described.

PARTICLE ASYMMETRY ANALYSER

ASYMMETRY PARTICLE ANALYSER An apparatus and method which provides a measure of the asymmetry as well as the size of individual fluid borne particles. Laser-light scattering techniques are employed to obtain data on the particles, which is then compared to data on known particle shapes to ascribe an asymmetry factor to the particles.

A MULTI-SPECTRAL OPTICAL METHOD AND SYSTEM FOR DETECTING AND CLASSIFYING BIOLOGICAL AND NON-BIOLOGICAL PARTICLES

Enhanced methods, apparatuses and systems are disclosed for the real-time detection and classification of biological and non-biological particles by substantially simultaneously measuring a single particle's characteristics in terms of size and density, elastic scattering properties, and absorption and fluorescence.

BLOOD CELL ANALYZER

DEVICE AND SYSTEM FOR COLLECTING AND ANALYZING VAPOR CONDENSATE, PARTICULARLY EXHALED BREATH CONDENSATE, AS WELL AS METHOD OF USING THE SAME

The present invention is related to the field of bio/chemical sensing, assays and applications. Particularly, the present invention is related to collecting a small amount of a vapor condensate sample (e.g. the exhaled breath condensate (EBC) from a subject of a volume as small as 10 fL (femto-Liter) in a single drop), preventing or significantly reducing an evaporation of the collected vapor condensate sample, analyzing the sample, analyzing the sample by mobile- phone, and performing such collection and analysis by a person without any professionals.

SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS

A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: distributing a population of cells and a population of non-cell particles across the array of wells through the fluid reservoir to increase capture efficiency of individual cell-particle pairs within the array of wells, and processing the captured cell-particle pairs at the set of wells.

DETECTING MICROSCOPIC OBJECTS IN FLUIDS

A method (10) utilizes first, second, and third image data originating from first, second, and third digital image frames, respectively, captured sequentially in time of a sample volume containing a fluid possibly comprising moving microscopic objects while illuminating the sample volume by coherent light, each image data comprising, for a moving microscopic object of foreign object present in the sample volume, a hologram pattern (11); and comprises automatically generating first differential image data comprising the difference of the first and the second image data, (13a);automatically generating second differential image data comprising the difference of the second and the third image data (13b); automatically generating product of difference (POD) image data comprising the product of the first and the second differential image data, (14); and automatically detecting the presence of moving microscopic object(s) in the sample volume on the basis of product pattern(s) present in the POD ...

SYSTEM FOR CONTROLLING AN EMULSIFICATION PROCESS

A system (1A) and method (IB) for controlling an emulsification process including the steps of acquiring (9) images (3) such as micrographs (2) of an emulsification process at preset intervals between a start and an end of the emulsification process; detecting (10) selected droplet characteristics such as size and count using image segmentation such as a histogram-based technique (5); analysing (11) the measured droplet characteristics (6); comparing (12) the measured droplet characteristics with a desired droplet characteristic specification(S); and terminating the emulsification process when said desired droplet characteristic is achieved.

ENVIRONMENTAL SYSTEM ON MODULE APPARATUS

An environmental system on a module apparatus for detecting and measuring particles entrained in an air stream. The apparatus includes a highly-integrated silicon circuit board. The circuit board has a plurality of embedded sensors, signal conditioning and processing. The plurality of sensors to detect pollution and other aspects of the physical environment. The apparatus also including a mechanical structure surrounding the top side of the circuit board. The structure forms an air pathway having an inlet and an outlet and a laser beam pathway having an emitter end and a beam line termination end. The air pathway intersects the laser beam pathway. A highly miniaturized optical particle scattering detector is located proximal to the intersection. A laser is located in the emitter end of the laser beam pathway.

SYSTEM AND METHOD FOR CHARACTERIZING PARTICULATES IN A FLUID SAMPLE

A system for characterizing at least one particle from a fluid sample is disclosed. The system includes a filter disposed upstream of an outlet, and a luminaire configured to illuminate the at least one particle at an oblique angle. An imaging device is configured to capture and process images of the illuminated at least one particle as it rests on the filter for characterizing the at least one particle. A system for characterizing at least one particle using bright field illumination is also disclosed. A method for characterizing particulates in a fluid sample using at least one of oblique angle and bright field illumination is also disclosed.

SYSTEMS AND METHODS TO FACILITATE GENETIC RESEARCH

Systems and methods that facilitate genetic research are described. The systems and methods can utilize (1) fluorescent dyes to sort tetrads from vegetative cells, dyads and dead cells; (2) natural genetic sequences to capture tetrad relationships of recombinant progeny; and (3) markers in parental organisms to identify genetic recombination events in genomic regions of interest.

CELL SORTING USING A HIGH THROUGHPUT FLUORESCENCE FLOW CYTOMETER

In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics ...

ULTRA-HIGH THROUGHPUT DETECTION OF FLUORESCENT DROPLETS USING TIME DOMAIN ENCODED OPTOFLUIDICS

A high-throughput optofluidic device for detecting fluorescent droplets is disclosed. The device uses time-domain encoded optofluidics to detect a high rate of droplets passing through parallel microfluidic channels. A light source modulated with a minimally correlating maximum length sequences is used to illuminate the droplets as they pass through the microfluidic device. By correlating the resulting signal with the expected pattern, each pattern formed by passing droplets can be resolved to identify individual droplets.

AUTOMATED COLLECTION OF A SPECIFIED NUMBER OF CELLS

Embodiments of the disclosed subject matter provide an automated method and system to isolate and collect cells using computerized analysis of images of cells and their surroundings obtained from a digital imaging device or system. Embodiments of the disclosed subject matter make use of a "microwell array," which can comprise a formed, elastomeric grid of indentations or "wells." Many, most, or all of the wells in a microwell array can contain a releasable, microfabricated element, which can be referred to as a "raft." Embodiments of the disclosed subject matter provide a system and method for cell collection that includes computerized identification and collection of rafts with isolated single cells or a specific group or groups of cells, eliminating the need for continuous human identification and selection.

DOSIMETERS INCLUDING LENSLESS IMAGING SYSTEMS

FRACTIONATION OF PARTICLES

A fractionation system comprising means for forming a three dimensional optical lattice that is operable to separate particles that have different physical characteristics. Preferably, the wells of the optical lattice are interlinked. For example, the wells may be linked in such a manner as to provide deflection greater than or equal to 15 degrees.

FAST THERMO-OPTICAL PARTICLE CHARACTERISATION

The present invention relates to a method and an apparatus for a fast thermo-optical characterisation of particles. In particular, the present invention relates to a method and a device to measure the stability of (bio)molecules, the interaction of molecules, in particular biomolecules, with, e.g. further (bio)molecules, particularly modified (bio)molecules, particles, beads, and/or the determination of the length/size (e.g. hydrodynamic radius) of individual (bio)molecules, particles, beads and/or the determination of length/size (e.g. hydrodynamic radius).

IMPROVEMENTS TO PARTICLE DETECTORS

A beam detector (10) including a light source (32), a receiver (34), and a target (36), acting in cooperation to detect particles in a monitored area (38). The target (36), reflects incident light (40), resulting in reflected light (32) being returned to receiver (34). The receiver (34) is a receiver is capable of recording and reporting light intensity at a plurality of points across its field of view. In the preferred form the detector (10) emits a first light beam (3614) in a first wavelength band; a second light beam (3618) in a second wavelength band; and a third light beam (3616) in a third wavelength band, wherein the first and second wavelengths bands are substantially equal and are different to the third wavelength band.

HOLOGRAPHIC DEVICE AND OBJECT SORTING SYSTEM

A device for extracting at least one object characteristic of an object (106) is presented, the device comprising: a light sensor (101) for recording a hologram of an object and a processing unit (102) coupled to the light sensor and configured for extracting at least one object characteristic from the hologram; wherein the processing unit is configured for extracting the at least one object characteristic from a section of the hologram without reconstructing an image representation of the object. Further, a device (200) for sorting an object (106), a method for identifying an object and a method for sorting objects is presented.

MULTIPLEX BEAD ARRAY ASSAY

The present disclosure relates to a system, method, and kit for particle detection and analysis. Devices disclosed herein may include at least an optical source, a fludic chip containing a multiplex bead array, and a detection module, wherein the sample flows within the fludic chip past a detection window, where the cells or particles are imaged by an image acquisition and analysis module that may include an optical detector. The image acquisition and analysis module counts the labeled particles and software allows for analysis of bead population.

METHOD AND DEVICE FOR DETERMINING THE ORIENTATION OF PIGMENT PARTICLES OVER AN EXTENDED REGION OF AN OPTICALLY EFFECT LAYER

The present invention relates to the field of the protection of security documents and more particularly to a method and a device for determining the distribution and orientation of platelet-shaped pigment particles over an extended region of an optical effect layer (OEL). The method described herein comprises the steps of a) taking at least one image, under illumination of said extended region of the optical effect layer with collimated light incident from at least one first direction, of reflected light of said extended region of the optical effect layer from at least one second direction, using a telecentric lens-and-camera assembly having the optical axis of the telecentric lens oriented along said second direction, and b) processing the at least one image of said extended region to extract quantitative particle distribution and orientation information. The device described herein comprises a) a collimated light source for illuminating an extended region of the optical effect layer ...

FLUID STREAM IMAGING APPARATUS

A fluid stream imaging apparatus having either optics for manipulating the aspect ratio or sensing elements configured for manipulating the aspect ratio of an image of the fluid stream. This application may also relate to a system for acquiring images of a portion of a fluid stream at high speeds for image processing to measure and predict droplet delays for individual forming particles.

OPTICAL SECTIONING OF A SAMPLE AND DETECTION OF PARTICLES IN A SAMPLE

The invention relates to an apparatus, a method and a system for obtaining a plurality of images of a sample arranged in relation to a sample device. The apparatus comprises at least a first optical detection assembly having an optical axis and at least one translation unit arranged to move the sample device and the first optical detection assembly relative to each other. The movement of the sample device and the first optical detection assembly relative to each other is along a scanning path, which defines an angle theta relative to the optical axis, wherein theta is larger than zero.

PARTICLE DETECTOR

A smoke detector is disclosed which uses a beam of radiation such as a laser, to monitor a region, such as a room. A camera is used to capture images of part of the room, including a path of the laser beam. Particles in the laser beam scatter light, and this is captured by the camera for analysis. A processor extracts data relating to the scattered light to determine the density of particles in the beam, to determine the level of smoke in the region. The laser may have a modulated output so that images captured without the laser can be used as a reference point and compared to images taken with the laser on, to assist in determining the level of scattered light compared to ambient light. Filters may be used to decrease signals generated from background light.

NOZZLE ASSEMBLY FOR A FLOW CYTOMETER SYSTEM AND METHODS OF MANUFACTURE

A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbling a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.

METHOD AND APPARATUS FOR MONITORING THE DIAMETER OF THERMALLY-SPRAYED PARTICLES

In a method of monitoring in-flight particles, a mask in a is places in a plane generally parallel to the direction of travel of the particles. The mask defines at least two slits that are generally parallel and lie in a direction having at least a component at right angles to the direction of travel. The have their ends offset relative to each other. The radiation emitted, scattered or absorbed by the particles is monitored as they traverse the slits. The particles for which the radiation, emitted, scattered or absorbed at each slit as they sequentially traverse said slits bears a predetermined relationship are identified, and the size of the identified particles is determined from the amount of radiation emitted, scattered or absorbed as the identified particles traverse the slits.

Appareil pour identifier diverses particules microstructurées à l'intérieur d'un fluide

MECHANISM FOR MEASURING THE FLUORESCENCE OF CELLS.

Device for registering the composition of foreign particles in liquids

The device contains a feed duct (2) and a registration duct (4), which communicate with one another via a nozzle (3) and through which the test liquid is pumped. Provided in the registration duct (4) there are transparent windows (5) and (6) for passing a luminous flux from the illumination apparatus (7) through the flowing liquid. The device further contains a photosensitive unit (8) for registering the light reflected by the particles present in the liquid. The ratio of the diameters of the nozzle (3) and of the registration duct (4) is from 1/4 to 1/7. The main purpose of the device is the recording of dirt particles in fuels and oils. ...

SENSOR FOR THE PHOTOELECTRIC SCANNING OF THE DIMENSIONS AND NUMBER OF PARTICLES CONTAINED IN A GAS JET.

Apparatus for inspecting samples of cells by means of fluorescence.

Die Erfindung betrifft einen Zellanalyse-Apparat, bestehend aus a. einem Probenträger (10), der eine Zellprobe enthält, welche mit mindestens einem fluoreszierenden Agens behandelt wurde; b. einem Probenmess-Mittel, bestehend aus (i) mindestens einem Probenbeleuchtungs-Mittel (101, 102), wobei das mindestens eine Probenbeleuchtungs-Mittel (101, 102) aus einer Lichtquelle besteht, um die Probe zu beleuchten; (ii) einem Detektions-Mittel (103), bestehend aus einer ferngesteuerten Fokussierlinse (15), die darauf ausgelegt ist, die fluoreszente Strahlung, die von der Probe emittiert wird, zu empfangen; und d. einem Kontroll- und Datenanalyse-Mittel (1), das darauf ausgelegt ist, die funktionellen Operationen des Probenmess-Mittels zu kontrollieren und Daten zu empfangen, analysieren und Resultate zu präsentieren, wobei das Kontroll- und Datenanalyse-Mittel (1) getrennt von der Probe und vom Probenmess-Mittel vorliegt. Der Apparat ist leicht und transportierbar.

mikrozhidko holistic and flow analyzer for detection of pathology and method of detecting pathology

VISUALIZATION SYSTEM FOR COUNTING AND OF DETERMINING PARTICLE SIZE IN FILLED WITH FLUID MEDIUM VESSELS

SPECIFIC MALARIA DETECTION WITH DIGITAL HOLOGRAPHIC MICROSCOPY

The particle counter

DISPOSITIF DE MESURE DE PROPRIETES DE CELLULES

L'invention a trait à un dispositif de mesure des propriétés de cellules. Elle a pour objet un dispositif de mesure de cellules en suspension dans un liquide et passant avec celui-ci par des points de mesure réalises dans un canal d'une chambre d'écoulement et pouvant être examinés à travers une fenêtre de la paroi du canal, par excitation de fluorescence à l'aide de deux sources lumineuses de longueurs d'onde différentes, une source de lumière étant associée à un point de mesure. Les points de mesure 20, 22 sont réalisés en un coude 12 du canal d'écoulement, la section d'arrivée et la section de départ formant chacun un des points de mesure séparés par l'angle inclus du coude 16, 18. Application : analyses biologiques.

DEVICE AND PROCESS Of MICROSCOPIC ANALYSIS MULTIPARAMETRIQUE Of ELEMENTS

METHOD AND DEVICE FOR OBSERVING A SAMPLE UNDER AMBIENT LIGHT

DEVICE FOR EXAMINING A FLUID BY UNIFORM ILLUMINATION USING A CONFIGURED LIGHT GUIDE

DEVICE AND PROCESS OF MEASUREMENT OF PHOTOLUMINESCENCE, ABSORPTION AND MICROSCOPIC DEDIFFRACTION Of OBJECTS IN a FLUID.

L'invention concerne un dispositif et un procédé de mesure de photoluminescence dans un fluide présent dans une cuve de mesure [CM]. Selon l'invention, le fluide dans la cuve de mesure [CM] reçoit simultanément au moins deux faisceaux d'excitation en provenance de deux systèmes optiques [Ci]. Chaque système optique [Ci] comprend chacun une source [Si] de lumière de faible cohérence spatiale envoyant ledit faisceau d'excitation vers la cuve de mesure [CM] selon un axe [Xi] dit du système [Ci] et un élément de capture [CEi] destiné à recevoir un faisceau d'émission de fluorescence centré sur l'axe [Xi] du système [Ci] en provenance du fluide. Ces systèmes optiques [Ci] sont positionnés pour que leurs axes [Xi] fassent entre eux un angle non nul et distinct de 180° autour de la cuve de mesure [CM]. Une mesure de photoluminescence est déduite selon l'invention d'un couplage de données obtenues à partir des faisceaux d'émission capturés simultanément par les éléments de capture [CEi].

COMPOSITION FOR THE OPTICAL DETECTION OF PARTICLES, COMPRISING WATER, THE PARTICLES, A SURFACTANT AND A HYDROPHILIC POLYMER, SYSTEM AND METHOD FOR OPTICALLY SENSING ASSOCIES

Cette composition (28) pour la détection optique de particules (30) est destinée à être agencée sur une lame transparente (26) pour l'acquisition d'au moins une image des particules (30) à l'aide d'un système de détection optique (20). La composition (28) comprend de l'eau, les particules (30) et un surfactant, les particules (30) présentant un diamètre de préférence inférieur à 10 µm, de préférence encore inférieur à 1 µm, le surfactant présentant une concentration de préférence au moins égale à la concentration micellaire critique. La composition (28) comprend en outre un polymère hydrophile, le polymère hydrophile présentant une température d'ébullition supérieure à celle de l'eau.

METHOD FOR OBSERVING AN OBJECT

L'invention est un dispositif et un procédé pour observer un objet, en particulier un objet biologique. Le dispositif comporte une source de lumière apte à illuminer un échantillon. Sous l'effet de l'illumination, l'objet émet un rayonnement rétrodiffusé se propageant jusqu'à un écran, dont l'aire est supérieure à 100 cm2. La projection du rayonnement diffusé sur l'écran forme une image représentative du rayonnement diffusé, désignée par le terme diffractogramme. Un capteur d'image permet une acquisition d'une image représentative du diffractogramme formé sur l'écran.

Modulation pulsatoire de la source de lumière d'excitation de cytomètres à écoulement.

Procédé pour augmenter l'intensité de la lumière d'excitation et de ce fait la sensibilité, de cytomètres à écoulement. La partie initiale du signal produit par chaque cellule, lorsqu'elle pénètre dans le foyer d'excitation (2) déclenche une impulsion de courant envoyée à la source de lumière d'excitation (1), dont la durée est sensiblement semblable au temps exigé par la cellule pour passer à travers ce foyer (2). Cette impulsion de courant, qui se superpose au courant de fonctionnement constant de cette source de lumière (1), provoque une augmentation de l'intensité de la lumière d'excitation pendant la période pendant laquelle la cellule traverse le foyer d'excitation (2), et de ce fait provoque une augmen tation de la sensibilité du cytomètre à écoulement.

PULSATING MODULATION OF THE EXCITATION LIGHT SOURCE OF FLOW CYTOMETERS.

IMAGING SYSTEM WITHOUT LENS COMPRISING A DIODE, A DIAPHRAGM AND A DIFFUSER BETWEEN THE DIODE AND THE DIAPHRAGM

Ce système d'imagerie (20) comprend un support de réception (26) configuré pour recevoir un échantillon (24), une source de lumière (30) configurée pour émettre un faisceau lumineux (50) d'éclairement de l'échantillon (24) selon une direction d'éclairement (Z), la source de lumière (30) comportant une diode (52) et un diaphragme (54), le diaphragme (54) étant disposé entre la diode (52) et le support de réception (26) selon la direction d'éclairement (Z), et un photodétecteur matriciel (32) configuré pour acquérir au moins une image de l'échantillon (24), chaque image étant formée par un rayonnement transmis par l'échantillon éclairé (24) et comportant au moins une figure de diffraction élémentaire, le support de réception (26) étant disposé entre la source de lumière (30) et le photodétecteur matriciel (32) selon la direction d'éclairement (Z). Le système (20) comprend en outre un diffuseur de lumière (56) disposé entre la diode (52) et le diaphragme (54).

PROTECTION DEVICE CAN BE INSERTED INTO A DETECTION SYSTEM BY LENSLESS IMAGING AND DETECTION SYSTEM BY LENSLESS IMAGING EMPLOYING SAME

L'invention concerne un dispositif de protection destiné à être employé dans un système de détection d'objets d'intérêt dispersés dans un échantillon, ledit système comportant : - une source (1) de rayonnement lumineux destinée à émettre un rayonnement lumineux (10) suivant une direction principale (X), - un capteur (2) d'image, - une zone de visualisation (Z) transparente destinée à recevoir ledit échantillon et positionnée entre ladite source (1) de rayonnement lumineux et ledit capteur (2), et ledit capteur (2) étant agencé pour acquérir sur sa surface une image de l'échantillon à partir d'un rayonnement transmis à travers la zone de visualisation par ladite source de rayonnement, - ledit dispositif comportant des moyens de protection à intercaler entre la source (1) de rayonnement lumineux et ladite zone de visualisation (Z) pour protéger ladite zone de visualisation du dépôt d'impuretés.

BLOOD CELL ANALYZER

SYSTEM AND METHOD FOR OPTICAL DETECTION OF OBJECTS IN A FLUID

Turbidity mesuring probe with macromolecule membrane modified by hydrophobic sol-gels

Optical configuration method for spectral-scattered oil-cell analysis

URINE FIELD INSPECTION DEVICE AND METHOD

A urine capturing apparatus is to receive urine from a user on a toilet, and a chamber is fluidically coupled with the capturing apparatus. A diverter is fluidically coupled between the capturing apparatus and the chamber. The diverter is to divert the direction of the received urine toward the chamber. A detection unit is to sense the presence of predetermined characteristics in the urine and to generate at least one electrical signal comprising information of the predetermined characteristics. COPYRIGHT KIPO 2016 (102) Storing urine of the person(For, example, during using a toilet chair) (104) Collecting an urine sample in a chamber (106) Detecting the exisitance of the fixed properties of the urine samples (108) Generating electric signal including information related to the fixed properties (110) Inspecting clean level for penetrating a washing solution in a system and inspecting the next urine sample ...

PARTICLE COUNTER

A method and system for determination of starch in a sample

In a starch concentration measurement, a liquid sample is conducted from a liquid sample such as pulp suspension or filtrate of a paper, board or tissue process (20). An iodine solution is added to the sample (22), and a light absorbance or transmittance of the sample is measured at a wavelength longer than about 650 nm, preferably longer than about 700 nm (24). The measured absorbance or transmittance of the sample is converted into the starch concentration of the sample by means of a predefined correlation between a starch concentration and a light absorbance or transmittance (26).

Achromatic astigmatic anamorphic objective

An anamorphic three-element objective lens projects a plurality of beams of different wavelengths and different diameters into an elongated focal spot in a working-plane. In one transverse direction of the lens, the beams are tightly focused with equal beam-waist widths in the working-plane, defining a height of the focal spot. In another transverse direction, the different beams are focused progressively beyond the working- plane such that the beams have a common beam-width in the working-plane, thereby defining a width of the focal spot.

Inline particle sensor

Minute particle analyzing device and method

A minute particle analyzing device includes: a light source; a first condenser lens for condensing light from the light source to a first end of a multimode optical fiber; a second condenser lens for condensing the light emerging from a second end of the multimode optical fiber to a minute particle; and a detector for detecting light generated from the minute particle by the application of the light from the light source.

Fluorescence detection device and fluorescence detection method

When receiving fluorescence emitted by a measurement object irradiated with laser light emitted from a laser light source unit, a fluorescence detection device generates a modulation signal for modulating the intensity of the laser light and modulates the laser light using the modulation signal. The fluorescence detection device obtains a fluorescent signal of the fluorescence emitted by the measurement object irradiated with the laser light, and calculates, from the fluorescent signal, a fluorescence intensity and the phase delay of the fluorescence with respect to the modulation signal. At the time, the fluorescence detection device controls the operation amounts of the signal level of a DC component of the modulation signal and the gain of amplification just after the output of the fluorescent signal so that the value of a fluorescence intensity signal falls within a preset range. After the operation amounts are settled, the fluorescence detection device calculates the fluorescence intensity and then calculates the fluorescence relaxation time of the fluorescence emitted by the measurement object using the phase delay.

particle detectors

A beam detector ( 10 ) including a light source ( 32 ), a receiver ( 34 ), and a target ( 36 ), acting in co-operation to detect particles in a monitored area ( 38 ). The target ( 36 ), reflects incident light ( 40 ), resulting in reflected light ( 32 ) being returned to receiver ( 34 ). The receiver ( 34 ) is a receiver is capable of recording and reporting light intensity at a plurality of points across its field of view. In the preferred form the detector ( 10 ) emits a first light beam ( 3614 ) in a first wavelength band; a second light beam ( 3618 ) in a second wavelength band; and a third light beam ( 3616 ) in a third wavelength band, wherein the first and second wavelengths bands are substantially equal and are different to the third wavelength band.

Minimally invasive cytometry system with qcl inspection of single cells for cancer detection

This disclosure concerns a minimally invasive cytometry system including a handling system that presents single cells to at least one QCL laser source. The QCL laser source is configured to deliver light to a cell within the cells in order to induce vibrational bond absorption in one or more analytes within the cell. The cytometry system also includes a detection facility that detects the mid-infrared wavelength light transmitted by the cell and identifies the cell as either cancerous or non-cancerous.

White Blood Cell Analysis System and Method

Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WB Cs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

Fine particle measuring apparatus

A fine particle measuring apparatus is provided. The fine particle measuring apparatus includes a detection unit configured to detect light emitted from a fine particle and a processing unit having a memory device storing instructions which when executed by the processing unit, cause the processing unit to calculate a corrected intensity value of the detected light and generate spectrum data based on the corrected intensity value.

Method and apparatus for measuring optical properties of particles of a dispersion

Disclosed is apparatus for measuring optical properties of particles of a flowable dispersion using a measuring cuvette. The dispersion flows through the central inner chamber of the cuvette. Two laser light beams, which are offset 90 degrees to one another, illuminate the inner chamber of the cuvette, so as to illuminate a particle, regardless of its orientation, in a way that balances out form factor errors.

Convex Lens-Induced Confinement for Measuring Distributions of Molecular Size

A curved surface is placed tangent to a slide and displaces a sample liquid from the point or line of contact outward. Imaging indicates a region where fluorescence is observed, and the location of the fluorescence indicates the molecular size. The radius of curvature of the lens is known, the distance from the (center) point of contact of the observed fluorescence is measured with a microscope and the distance of the lens surface to the slide's surface can then be calculated. This distance represents the size of the molecule or ensemble of molecules emitting. Similarly, absorbance, etc. could be measured with a light source below the slide.

Spatially Correlated Light Collection from Multiple Sample Streams Excited with a Line Focused Light Source

An affordable flow cytometry system with a significantly increased analytical rate, volumetric sample delivery and usable particle size including a light beam that interrogates multiple flow streams so as to provide excitation across all of the streams, and an optical objective configured to collect light from the sample streams and image the light onto an array detector.

System and Method for Measuring Particles in a Sample Stream of a Flow Cytometer Using Low-Power Laser Source

A system and method for analyzing a particle in a sample stream of a flow cytometer or the like. The system has a light source, such as a laser pointer module, for generating a low powered light beam and a fluidics apparatus which is configured to transport particles in the sample stream at substantially low velocity through the light beam for interrogation. Detectors, such as photomultiplier tubes, are configured to detect optical signals generated in response to the light beam impinging the particles. Signal conditioning circuitry is connected to each of the detectors to condition each detector output into electronic signals for processing and is designed to have a limited frequency response to filter high frequency noise from the detector output signals. 1. A particle interrogation system comprising:a flow chamber;a fluid container in connection with the flow chamber, the fluid container configured to deliver a gravity fed fluid to the flow chamber;a laser source configured to impinge a laser beam on one or more particles flowing in the flow chamber;a particle delivery system operably coupled to the flow chamber and configured to deliver a sample fluid containing one or more particles in the flow chamber,the particle delivery system being configured to provide a transit time of between at least about 100 microseconds to about 1 millisecond for a particle transported through the laser beam;at least one detector configured to receive one or more optical signals resulting from fluorescence or light scattered from the one or more particles; andsignal conditioning circuitry, operably coupled to the at least one detector.2. The system of claim 1 , wherein the laser source comprises a non-stabilized compact laser.3. The system of claim 1 , wherein the fluid container is connected to the flow chamber via a gravity fed sheath delivery line.4. The system of claim 1 , wherein the signal conditioning circuitry comprises a pre-amplifier stage coupled to the output of the ...

Optical particle detecting device and particle detecting method

An optical particle detecting device including a light source that emits an inspection light, a converting unit that converts the inspection light into collimated light, a focusing reflecting mirror that reflects toward a focal point the inspection light that has been converted into collimated light, a jet mechanism that causes an airstream including a particle to jet into the focal point of the focusing reflecting mirror, and a detecting portion that detects either scattered light or fluorescence produced by the particle included in the airstream being illuminated by the inspection light.

Nucleic Acid Analysis by Random Mixtures of Non-Overlapping Fragments

The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.

Nucleic Acid Analysis by Random Mixtures of Non-Overlapping Fragments

The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered. In one aspect, this process is carried out in a hierarchical fashion until the one or more target polynucleotides are characterized, e.g. by their nucleic acid sequences, or by an ordering of sequence segments, or by an ordering of single nucleotide polymorphisms (SNPs), or the like.

Method and Apparatus for Tracking a Particle, Particularly a Single Molecule, in a Sample

For the purpose of tracking a movement of a particle in a sample, the particle is driven by light to emit photons, and the photons emitted by the particle are detected. The light applied to the sample features a light intensity distribution with a spatially limited minimum. The particle is tracked with the minimum of the light intensity distribution by moving the light intensity distribution with respect to the sample such that a rate of photons emitted by the particle remains minimal, and by taking an actual position of the minimum of the light intensity distribution in the sample as an actual position of the particle in the sample.

Nozzle assembly for a flow cytometer system and methods of manufacture

A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbling a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.

MICROPARTICLE ANALYSIS APPARATUS AND MICROPARTICLE ANALYSIS METHOD

There is provided a microparticle analysis apparatus including a light detection unit configured to detect forward-scattered light generated from a microparticle that is an analysis target. The light detection unit includes a circuit having a high-pass filter that removes low frequency noise included in light entering the light detection unit and switches to the high-pass filter according to a predetermined frequency of the forward-scattered light. 1. A microparticle analysis apparatus comprising:a light detection unit configured to detect forward-scattered light generated from a microparticle that is an analysis target,wherein the light detection unit includes a circuit having a high-pass filter that removes low frequency noise included in light entering the light detection unit and switches to the high-pass filter according to a predetermined frequency of the forward-scattered light.2. The microparticle analysis apparatus according to claim 1 , wherein the light detection unit switches to the high-pass filter when the predetermined frequency is equal to or higher than 200 kHz.3. The microparticle analysis apparatus according to claim 2 , wherein the high-pass filter removes low frequency noise included in leakage light which avoids a zero-order light removal unit arranged between the microparticle and the light detection unit and which enters into the light detection unit.4. The microparticle analysis apparatus according to claim 3 , wherein the high-pass filter removes noise of a frequency lower than 2 kHz.5. The microparticle analysis apparatus according to claim 4 ,wherein the circuit includes a channel directly connected from an input side to an output side and a channel having the high-pass filter, andwherein the channel directly connected and the channel having the high-pass filter are connected in parallel.6. The microparticle analysis apparatus according to claim 5 , wherein the circuit includes a switch element to perform switching to one of the channel ...

DEVICES AND METHODS FOR FRACTIONATED PHOTOACOUSTIC FLOW CYTOMETRY

A fractionated photoacoustic flow cytometry (PAFC) system and methods for the in vivo detection of target objects in biofluidic systems (e.g., blood, lymph, urine, or cerebrospinal fluid) of a living organism is described. The fractionated system includes a fractionated laser system, a fractionated optical system, a fractionated acoustic system, and combinations thereof. The fractionated laser system includes at least one laser or laser array for pulsing a target object within the circulatory vessel with fractionated focused laser beams. The fractionated optical system separates one or several laser beams into multiple beams in a spatial configuration on the skin above the circulatory vessel of the living organism. The fractionated acoustic system includes multiple focused ultrasound transducers for receiving photoacoustic signals emitted by the target object in response to the fractionated laser beams. The target objects have intrinsic photoacoustic contrast or may be labeled with photoswitchable or spaser-based probes. Fractioned beams may be used also for diagnostics with other spectroscopic methods (e.g., fluorescence, Raman or scattering) and energy sources both coherent and conventional such as lamp and LED in the broad spectral range from 10 Å to 1 cm (e.g., X-ray, UV, visible, NIR or microwaves) in continuous wave and pulse modes. 1. A fractionated photoacoustic flow cytometry system for the in vivo detection of target objects in a biofluid system of a living organism , comprising:a laser system comprising at least one laser comprising at least one wavelength for providing at least one laser beam to at least one target object within the biofluid system;a fractionated optical system configured to separate the at least one laser beam into fractionated laser beams having a spatial configuration on the skin above the biofluid system of the living organism; andan acoustic system comprising at least one focused ultrasound transducer for receiving more than one ...

Systems And User Interface For Collecting A Data Set In A Flow Cytometer

Systems in a flow cytometer having an interrogation zone and illumination impinging the interrogation zone include: a lens subsystem including a collimating element that collimates light from the interrogation zone, a light dispersion element that disperses collimated light into a light spectrum, and a focusing lens that focuses the light spectrum onto an array of adjacent detection points; a detector array, including semiconductor detector devices, that collectively detects a full spectral range of input light signals, in which each detector device detects a subset spectral range of the full spectral range of light signals; and a user interface that enables a user to create a set of virtual detector channels by grouping detectors in the detector array, such that each virtual detector channel corresponds to a detector group and has a virtual detector channel range including the sum of subset spectral ranges of the detectors in the corresponding detector group. 19-. (canceled)10. A light detection system comprising:a plurality of user-configurable groups of photodetectors; anda plurality of detector channels, wherein each detector channel is assigned to one or more of the user-configurable groups of photodetectors.11. The light detection system according to claim 10 , wherein the light detection system is configured to:detect light from a sample irradiated with a light source; andgenerate a data signal from the detected light.12. The light detection system according to claim 11 , wherein the light detection system is operably coupled to a processor comprising memory having instructions stored thereon claim 11 , which when executed by the processor claim 11 , cause the processor to assign each detector channel to a user-configurable group of photodetectors before generating a data signal from the light detected from the irradiated sample.13. The light detection system according to claim 11 , wherein the light detection system is operably coupled to a processor ...

Filter and Blower Geometry for Particle Sampler

The invention provides devices and methods for sampling, detecting and/or characterizing particles. Devices and methods of the invention, including particle samplers, impactors and counters, include a filter component for removing particles in the exhaust flow of the device, for example, to eliminate or minimize the potential for the device itself to provide source of particles in an environment undergoing particle monitoring. This aspect of the present devices and methods is particularly useful for monitoring particles in manufacturing environments requiring low levels of particles, such as cleanroom environments for electronics manufacturing and aseptic environments for manufacturing pharmaceutical and biological products. 1. A sampler comprising:one or more fluid inlets for sampling a fluid flow;a particle analysis or collection region positioned in fluid communication with said one or more fluid inlets;a fan or pump positioned in fluid communication with said particle analysis or collection region, said fan or pump for generating said fluid flow, wherein said fan or pump comprises a motor; anda filter in fluid communication with said fan or pump and positioned around at least a portion of said motor, said filter for filtering said fluid flow exhausted from said fan or pump.2. (canceled)3. The sampler of claim 1 , wherein said filter has a toroid shape and said motor is positioned in a vacant central region of said toroid shape.4. The sampler of claim 1 , wherein said filter has a cylindrical shape and said motor is positioned in a central aperture of said cylindrical shape.5. The sampler of claim 4 , wherein said fan has a rotational axis and wherein said cylindrical shape has a cylindrical axis and wherein said rotational axis and said cylindrical axis are parallel.6. (canceled)7. The sampler of claim 1 , wherein said fluid flows through said one or more fluid inlets claim 1 , through said particle analysis or collection region claim 1 , into an intake of said ...

Analysis Device and Analysis Method

An analysis device optically scans a surface of a substrate to which analytes and particles for labeling the analytes are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the analytes and determines that the analyte count is one when two pulse waves are detected consecutively each having pulse width less than first reference value determined depending on first pulse width in the detection signal when the optical scanning unit scans a plurality of particles adjacent to each other. 1. An analysis device comprising:an optical scanning unit configured to optically scan a surface of a substrate to which analytes and particles for labeling the analytes are fixed;a pulse detector configured to detect a pulse wave and a pulse width of the pulse wave included in a detection signal obtained from the optical scanning unit when the optical scanning unit scans the substrate; anda counting unit configured to count the analytes and determine that an analyte count is one when the pulse detector consecutively detects two pulse waves each having a pulse width less than a first reference value.2. The analysis device according to claim 1 , wherein the counting unit determines that the analyte count is one when the pulse detector detects a pulse wave having a pulse width greater than or equal to the first reference value and less than a second reference value.3. The analysis device according to claim 1 , wherein claim 1 , when the pulse detector detects a first pulse wave having a pulse width less than the first reference value claim 1 , detects a second pulse wave after the first pulse wave claim 1 , and a pulse interval between the first pulse wave and the second pulse wave is greater than or equal to a third reference value claim 1 , the counting unit does not implement counting processing with regard to both the first pulse wave and the second pulse wave.4. The analysis ...

PARTICLE MEASURING DEVICE

A particle measuring device includes: an optical resonator that reflects laser light back and forth between two facing reflective mirrors in order to amplify an energy of that laser light and form resonant laser light; a particle transport unit that transports particles in an aerosol to be measured across a beam path of the resonant laser light; a scattered light receiving unit that receives scattered light produced when the particles in the aerosol are irradiated by the resonant laser light; and a processor that receives light reception signals from the scattered light receiving unit, wherein the processor outputs light reception pulses according to the light reception signals and calculates time intervals between the light reception pulses that are temporally adjacent. 1. A particle measuring device for measuring particles in an aerosol , comprising:an optical resonator that causes laser light to travel back and forth between two opposing reflective mirrors in order to amplify an energy of the laser light and form resonant laser light;a particle transport unit configured to transport the particles in the aerosol across a beam path of the resonant laser light so as to generate a stream of the particles crossing the beam path;a scattered light receiving unit configured to receive scattered light that is produced when the particles in the aerosol are irradiated by the resonant laser light, and output a light reception signal in accordance with the received scattered light for each scattering event; anda processor that receives the light reception signal from the scattered light receiving unit for each event of the reception of the scattered light,wherein the processor outputs a light reception pulse in accordance with each light reception signal from the scattered light receiving unit, and derives time intervals between light reception pulses that are temporally adjacent.2. The particle measuring device according to claim 1 , wherein the processor further derives a ...

PHOTODETECTION DEVICE

A photodetection device has an optical module () that includes a light source (), an excitation optical system, and a detection optical system and that two-dimensionally and relatively scans a transparent stage () in a first sampling direction and a second sampling direction intersecting the first sampling direction. A scan length in the first sampling direction is longer than a scan length in the second sampling direction. A data sampling unit in the detection optical system performs sampling for a distance of a second sampling interval during scanning in the second sampling direction, and performs sampling for a distance of a first sampling interval shorter than the distance of the second sampling interval during scanning in the first sampling direction. An aperture () of the excitation optical system sets a size in the first sampling direction of a spot shape of excitation light from the light source () to be smaller than a size in the second sampling direction. 1. A photodetection device comprising:a light-transmitting transparent stage on which a detection object is placed;an excitation optical system that irradiates the detection object with excitation light emitted from a light source;a detection optical system that detects light emitted from a detection surface of the detection object placed on the transparent stage by irradiation with the excitation light;a data sampling unit included in the detection optical system to sample an intensity of the detected light at a predetermined set interval; andan optical module that includes the light source, the excitation optical system, and the detection optical system and that two-dimensionally and relatively scans the transparent stage in a first sampling direction of the data sampling unit and a second sampling direction intersecting the first sampling direction,wherein a scan length in the first sampling direction in the optical module is longer than a scan length in the second sampling direction,wherein the data ...

METHOD OF AND APPARATUS FOR ASCERTAINING THE SIZE OF PARTICLES

A method of ascertaining the size of small particles is disclosed. The method includes the steps of: a) intersecting at least two light beams at an intersection volume; b) sensing at each of a plurality of sensing positions angularly displaced from one another light scattered by a particle substantially in the intersection volume, and producing respective output signals indicative of the sensed light; c) ascertaining the phase difference between one of the signals and each other of the signals to give a measured indication of the variation of phase difference with angular displacement; and d) comparing the measured indication with at least one known indication of the variation of phase difference with angle for a known particle size and thereby determining the size of the particle substantially in the intersection volume. 1. A method of ascertaining the size of small particles , the method including the steps of:a) intersecting at least two light beams at an intersection volume;b) sensing at each of a plurality of sensing positions angularly displaced from one another light scattered by a particle substantially in the intersection volume, and producing respective output signals indicative of the sensed light;c) ascertaining the phase difference between one of the signals and each other of the signals to give a measured indication of the variation of phase difference with angular displacement; andd) comparing the measured indication with at least one known indication of the variation of phase difference with angle for a known particle size and thereby determining the size of the particle substantially in the intersection volume.2. A method according to claim 1 , wherein the measured indication of the variation of phase difference with angular displacement includes an indication of the angular position of transitions between local maxima and minima of the phase difference.3. A method according to claim 2 , wherein the measured indication includes information ...

FLUIDIC FLOW CYTOMETRY DEVICES AND PARTICLE SENSING BASED ON SIGNAL-ENCODING

Microfluidic devices, systems and techniques in connection with particle sorting in liquid, including cytometry devices and techniques and applications in chemical or biological testing and diagnostic measurements. 1. A particle sorter for sorting particles in a fluid , comprising:a structure having an input channel connected at an actuation area to a plurality of output channels, wherein the particles in the fluid flow through the input channel to the actuation area, and each particle travels from the actuation area to one of the plurality of output channels, anda piezoelectric actuator for causing a flow disturbance in the actuation area in response to a control signal, wherein the flow disturbance operates to direct a particle along a trajectory to one of the plurality of output channels which is different than the output channel to which the particle would travel without the flow disturbance.2. The particle sorter of claim 1 , wherein the structure includes at least one of a polymer substrate claim 1 , a polydimethylsiloxine (PDMS) substrate claim 1 , or a glass substrate.3. The particle sorter of claim 2 , wherein the piezoelectric actuator is permanently bonded via UV ozone treatment to the PDMS substrate.4. The particle sorter of claim 1 , wherein the piezoelectric actuator is integrated with the structure.5. The particle sorter of claim 1 , further comprising a driver for generating the control signal.6. The particle sorter of claim 1 , wherein the control signal is a voltage signal having a controlled magnitude and frequency.7. The particle sorter of claim 1 , wherein the detection unit comprises a bank of filters for detecting a signal from the particle.8. The particle sorter of claim 1 , wherein the piezoelectric actuator includes a contact layer for coupling the piezoelectric actuator to the structure claim 1 , and further includes a piezoelectric layer for generating a signal to cause the flow disturbance in response to the control signal.9. The ...

SYSTEM AND METHOD FOR DROPLET DETECTION

Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel. 1. A method of droplet detection , the method comprising:generating a single-file stream of droplets in carrier liquid;combining at least one stream of spacing fluid with the single-file stream of droplets in carrier liquid;directing the combined streams to a detection channel using a spacing channel that tapers toward the detection channel, wherein a distance between adjacent droplets is increased as such droplets travel along the spacing channel toward the detection channel; anddetecting a signal from droplets passing through the detection channel.2. The method of claim 1 , wherein the step of generating includes a step of passing droplets through an alignment region of a sample inlet channel claim 1 , wherein a taper of the alignment region arranges droplets in single file before reaching a channel junction claim 1 , and wherein the step of combining includes a step of combining at least one stream of spacing fluid with the single-file stream of droplets in carrier liquid at the channel junction.3. The method of claim 2 , wherein the step ...

Particulate matter detector

Devices and methods for detecting particulate matter are described herein. One device includes a laser, a reflector, an ellipsoidal reflector, and a detector, wherein the laser is configured to emit a beam, the reflector is configured to reflect the beam toward the ellipsoidal reflector, and the ellipsoidal reflector has a first focal region located on a path of the reflected beam, and a second focal region located at a surface of the detector.

LASER SENSOR MODULE FOR PARTICLE DETECTION WITH OFFSET BEAM

A laser sensor module for detecting a particle density of particles, which includes: a laser; a detector; and a mirror. The laser is arranged to emit a laser beam to the mirror. A movement of the mirror is arranged to redirect the laser beam. The laser beam is displaced with respect to a rotation axis of the mirror such that a focus region of the laser beam is moving with a velocity having components normal and parallel to the optical axis of the redirected laser beam such that an angle between the parallel and the normal velocity component is at least a threshold angle of 2°. The detector is arranged to determine a self mixing interference signal of an optical wave within a laser cavity of the laser, the self mixing interference signal being generated by laser light of the laser beam reflected by at least one of the particles. 1. A laser sensor module for detecting a particle density of particles with a size of less than 20 μm , wherein the laser sensor module comprises:a laser;a detector; anda mirror rotatable about a rotation axis,wherein the laser beam is focused to a focus region,wherein the laser is arranged to emit a laser beam to the mirror,wherein a movement of the mirror is arranged to dynamically redirect the laser beam,wherein a direction of the redirected laser beam defines an optical axis,wherein the laser beam is displaced with respect to the rotation axis of the mirror such that the focus region of the laser beam is moving with a velocity comprising components normal and parallel to the optical axis of the redirected laser beam such that an angle α between the parallel velocity component with the normal velocity component is at least a threshold angle of 2°, andwherein the detector is arranged to determine a self mixing interference signal of an optical wave within a laser cavity of the laser, the self mixing interference signal being generated by laser light of the laser beam reflected by at least one of the particles.2. The laser sensor module ...

Fine particle measurement device

A fine particle measurement device includes a support stand (20) that has a groove (F) extending in a predetermined direction and is configured to support in the groove an observation container (10), which has an elongate shape and accommodates a liquid sample containing a fine particle therein such that an extending direction of the groove (F) coincides with a longitudinal direction of the observation container (10); and an imaging unit (40) that is configured to capture an image of the fine particle in the observation container (10) at a position where the support stand is out of a field of view, the observation container (10) being supported by the support stand (20).

SPECTRAL MICROSCOPE

In one implementation, a spectral microscope may comprise a substrate with a planar lens, the planar lens including a phase profile including an axial focus and an oblique focus, a light source to excite a signal of a particle among a plurality of particles, and a detector to receive light generated from the light source from the axial focus of the planar lens and a spectral color component of the excited signal of the particle from the oblique focus of the planar lens. 1. A spectral microscope , comprising:a substrate with a planar lens, the planar lens including a phase profile including an axial focus and an oblique focus;a light source to excite a signal of a particle among a plurality of particles; and light generated from the light source from the axial focus of the planar lens; and', 'a spectral color component of the excited signal of the particle from the oblique focus of the planar lens., 'a detector to receive2. The spectral microscope of claim 1 , wherein the plurality of particles are located in a fluid.3. The spectral microscope of claim 1 , wherein the spectral color component of the oblique focus corresponds to a wavelength of the excited signal.4. The spectral microscope of claim 1 , wherein the phase profile of the planar lens includes:an axial phase profile based on the axial focus of the planar lens; andan oblique phase profile based on the oblique focus of the planar lens.5. The spectral microscope of claim 1 , wherein the planar lens is a diffractive lens.6. The spectral microscope of claim 1 , wherein the light source is a light emitting diode.7. The spectral microscope of claim 1 , wherein the light source is a laser.8. A method claim 1 , comprising:generating, by a light source, an excitation light to excite a plurality of fluorescent signals of a plurality of particles of a fluid, wherein the fluid is located in a channel in a transparent chip;receiving, at a detector from an axial focus of a planar lens, the excitation light;receiving, at ...

MICROBIAL CYTOMETRIC MOCK COMMUNITIES AND USE THEREOF AS STANDARD IN FLOW CYTOMETRY

The present invention is directed to a microbial Cytometric Mock Community for use in flow cytometric analysis, the microbial Cytometric Mock Community comprising or consisting of cells of at least three different microbial species in a pre-defined ratio, wherein the at least three different microbial species are selected such that, when measured using flow cytometry, the specific gate pattern of each microbial species differs significantly from the specific gate pattern of the other microbial species of the microbial Cytometric Mock Community, preferably the at least three different microbial species differ in relative DNA content, relative genomic GC-content, relative cell size, Gram +/− affiliation and/or capacity to form spores. The microbial Cytometric Mock Community shall serve as standardization means that will help ecologists, microbiologists, molecular biologists and flow cytometrists to work on a standardized basis to allow comparison and exchange of data. 1. Microbial Cytometric Mock Community for use in flow cytometric analysis , the microbial Cytometric Mock Community comprising or consisting of cells of at least three different microbial species in a pre-defined ratio , wherein the at least three different microbial species are selected such that , when measured using flow cytometry , the specific gate pattern of each microbial species differs significantly from the specific gate pattern of the other microbial species of the microbial Cytometric Mock Community , preferably the at least three different microbial species differ in overall DNA content , relative genomic GC-content , average cell size , Gram +/− affiliation and/or capacity to form spores.2. Microbial Cytometric Mock Community of claim 1 , wherein the at least three different microbial species comprise or consist of species derived from archaea claim 1 , bacteria claim 1 , fungi claim 1 , protozoa and algae claim 1 , preferably derived from bacterial species.3. Microbial Cytometric Mock ...

Interferometric scattering microscopy

An interferometric scattering microscope is adapted by performing spatial filtering of output light, which comprises both light scattered from a sample location and illuminating light reflected from the sample location, prior to detection of the output light. The spatial filtering passes the reflected illumination light but with a reduction in intensity that is greater within a predetermined numerical aperture than at larger numerical apertures. This enhances the imaging contrast for coherent illumination, particularly for objects that are weak scatterers.

METHOD FOR CHARACTERISING A PARTICLE ON THE BASIS OF A HOLOGRAM

A method for characterizing a particle present in a sample, the sample lying between an image sensor and a light source and the sensor lying in a detection plane, includes illuminating the sample with the light source which emits an incident light wave propagating along a propagation axis, and acquiring an image of the sample with the sensor. The sensor is exposed to an exposure light wave. The image includes a plurality of elementary diffraction patterns each corresponding to one particle. The method also includes reconstructing a complex image representative of a complex amplitude of the light wave on a reconstruction surface passing through the sample, based on the acquired image; selecting a region of interest of the complex image corresponding to a particle of interest; forming an extracted image based on the region of interest; and characterizing the particle of interest depending on the extracted region of interest. 117-. (canceled)18. A method for characterizing a particle within a sample , the sample lying between an image sensor and a light source , the image sensor lying in a detection plane , the method comprising:a) illuminating the sample with the light source, the light source emitting an incident light wave that propagates along a propagation axis;b) acquiring an image of the sample with the image sensor, the image comprising a plurality of elementary diffraction patterns, each elementary diffraction pattern corresponding to one particle;c) on the basis of the acquired image, reconstructing a complex image representative of a complex amplitude of the exposure light wave on at least one reconstruction surface passing through the sample, the reconstruction being achieved by implementing an iterative reconstruction algorithm, the algorithm comprising, in each iteration, updating a phase of the exposure light wave in the detection plane or on the reconstruction surface;d) selecting a region of interest of the complex image, the selected region of ...

Optical Measurement System for Real-Time Process Monitoring of Aerosol Jet Printing

Aerosol jet printing is a popular digital fabrication method for flexible and hybrid electronics, but it lacks sophisticated process control architectures that would enable more widespread adoption in manufacturing environments. An optical measurement system can be used to track the aerosol density upstream of the printhead. For example, the measured optical extinction combined with the aerosol flow rate, is directly related to deposition rate and accurately predicts functional properties, for example electrical resistance. This real-time system offers a compelling solution for process drift and batch-to-batch variability, a valuable tool for more fundamental studies of the process science, and a viable technology to support real-time control of aerosol jet printing. 1. An aerosol jet printer , comprising:an atomizer for atomization of ink droplets, thereby providing an aerosol stream of aerosolized ink droplets;an optical measurement system, comprising a light source, an optics cell having a region for interaction of light from the light source with the aerosol stream, and a light sensor for detecting the light scattered from or transmitted through the aerosol stream in the interaction region, thereby providing an optical measurement of the aerosol stream; anda printhead downstream from the optical measurement system for printing of the ink droplets on a substrate.2. The aerosol jet printer of claim 1 , further comprising a closed-loop controller that uses a process metric to control at least one process parameter in real time.3. The aerosol jet printer of claim 2 , wherein the process metric comprises the product of an aerosol flow rate and an optical extinction of the aerosol stream.4. The aerosol jet printer of claim 2 , wherein the at least one process parameter comprises an atomizer voltage claim 2 , duty cycle claim 2 , temperature claim 2 , cartridge fill level claim 2 , ink composition claim 2 , aerosol flow rate claim 2 , sheath gas flow rate claim 2 , or ...

METHOD FOR DETECTING PARTICLES OR AEROSOL IN A FLOWING FLUID, COMPUTER PROGRAM, AS WELL AS ELECTRICAL MEMORY MEDIUM

A method for detecting particles or aerosol in a flowing fluid, using the principle of laser-induced incandescence. The method includes the following steps: a. focusing a laser light originating from a laser in a spot; b. conducting a fluid which includes particles or aerosol through the spot; c. detecting a thermal radiation originating from the spot with the aid of a detector; and d. evaluating a variable which is provided by the detector and characterizes the detected thermal radiation within time intervals, the duration of the time intervals being dependent on a velocity of the fluid. 114-. (canceled)15. A method for detecting particles or aerosol in a flowing fluid , using laser-induced incandescence , the method comprising the following steps:a. focusing a laser light originating from a laser in a spot;b. conducting the fluid which includes particles or aerosol through the spot;c. detecting a thermal radiation originating from the spot using a detector; andd. evaluating a variable which is provided by the detector and characterizes the detected thermal radiation within time intervals, a duration of the time intervals being dependent on a velocity of the fluid.16. The method as recited in claim 15 , wherein at least several of the time intervals overlap.17. The method as recited in claim 16 , wherein the duration of the time intervals is greater than an expected full width at half maximum (FWHM) of the variable characterizing the thermal radiation.18. The method as recited in claim 17 , wherein the duration of the time intervals is 1 to 2 times the expected FWHM.19. The method as recited in claim 18 , wherein the duration of the time intervals is 1.5 times the expected FWHM.20. The method as recited in claim 16 , wherein an overlapping time period of the time intervals corresponds to at least half the duration of the time interval.21. The method as recited in claim 16 , wherein a particle is considered to be detected when the variable characterizing the thermal ...

METHODS AND DEVICES FOR EVALUATING PERFORMANCE OF A DIODE LASER

Methods for evaluating performance a diode laser are provided. In embodiments, methods include receiving a laser beam profile of a diode laser, determining first, second and third laser beam widths at first, second and third laser intensities, respectively, for the laser beam profile, computing a first ratio between the second and third laser beam widths, computing a second ratio between the first and second laser beam widths, evaluating laser performance based on the first and second ratios, and outputting a determination regarding the suitability of the laser for use in a flow cytometry setting. Devices for practicing the subject methods are also provided, and include first and second stages configured to receive a diode laser and beam profiler, respectively. Aspects of the invention further include flow cytometers incorporating a diode laser that has been evaluated by the subject method. 1. A method for evaluating performance of a diode laser , the method comprising:receiving a laser beam profile of the diode laser, the laser beam profile comprising laser beam width data and laser intensity data;determining first, second and third laser beam widths at first, second and third laser intensities, respectively, for the laser beam profile;computing:a first ratio between the second and third laser beam widths; anda second ratio between the first and second laser beam widths; andevaluating laser performance based on the first and second ratios.2. The method according to claim 1 , wherein the diode laser is a semiconductor laser diode.35-. (canceled)6. The method according to claim 1 , wherein evaluating performance of the diode laser comprises assessing the extent to which the laser beam profile deviates from a Gaussian beam shape.7. The method according to claim 6 , wherein assessing the extent to which the laser beam profile deviates from a Gaussian beam shape comprises identifying whether multiple modes are present in the laser beam profile.8. The method according to ...

METHOD AND DEVICE FOR DETERMINING CHARACTERISTIC PROPERTIES OF A TRANSPARENT PARTICLE

The invention relates to a method for determining the size d of a transparent particle, according to which method the particle is illuminated with light from a light source, a radiation detector measures a time-resolved intensity profile of light of the light source scattered by the particle, a reflection peak () and a refraction peak are determined in the intensity profile and the size d of the particle is determined based on a time difference between the reflection peak () and the refraction peak. The method according to the invention is characterized in that the time-resolved intensity profile is measured at a definable scattering angle θs, a first second-order refraction peak () and a second second-order refraction peak () having a mode different from that of the first refraction peak () being determined, a characteristic variable γ being determined as the ratio of a first time difference Δtbetween the reflection peak () and the first refraction peak () and of a second time difference Δtbetween the reflection peak () and the second refraction peak (), and the size of only those particles being determined for which the characteristic variable γ corresponds to a definable value. 1. A method for determining characteristic properties of a transparent particle , wherein the particle is illuminated with light from a light source , wherein a time-resolved intensity profile of light from the light source that is scattered at the particle is measured by a radiation detector at a predefinable scattering angle θ , wherein characteristic scattered light peaks are determined in the intensity profile , and wherein a size of the particle is determined based on a time difference between two scattered light peaks ,wherein a first time difference is determined between a first pair of scattered light peaks and a second time difference is determined between a second pair of scattered light peaks, a characteristic variable is determined from the ratio of the first time difference ...

ANALYSIS DEVICE AND ANALYSIS METHOD

An analysis device optically scans a surface of a substrate to which particles are fixed, detects a pulse wave included in a detection signal obtained from an optical scanning unit when the optical scanning unit scans the substrate, and counts the particles based on pulse interval between two pulse waves each having pulse width less than first reference value determined depending on first pulse width when the optical scanning unit scans a plurality of particles adjacent to each other when the two pulse waves are detected consecutively. 1. An analysis device comprising:an optical scanning unit configured to optically scan a surface of a substrate to which particles are fixed;a pulse detector configured to detect a pulse wave and a pulse width of the pulse wave included in a detection signal obtained from the optical scanning unit when the optical scanning unit scans the substrate; anda counting unit configured to count the particles based on a pulse interval between two pulse waves each having a pulse width less than a first reference value when the pulse detector consecutively detects the two pulse waves.2. The analysis device according to claim 1 , wherein claim 1 , when the two pulse waves each having the pulse width less than the first reference value are consecutively detected by the pulse detector claim 1 , the counting unit counts the particles based on a number obtained by dividing the pulse interval between the two pulse waves by a first pulse width detected when the optical scanning unit scans the particles adjacent to each other.3. The analysis device according to claim 2 , wherein the first reference value is a sum of the first pulse width and a predetermined value included in the detection signal.4. The analysis device according to claim 1 , wherein the counting unit counts the particles and determines that a particle count is one when the pulse detector detects a pulse wave having a pulse width greater than or equal to the first reference value and less ...

COMPOUND OPTICAL FLOW CELLS AND METHOD OF MANUFACTURE AND USE

An improved optical flow cell adapted for use in a flow cytometer for differentiating formed bodies (e.g., blood cells) in liquid suspensions. Preferably manufactured by assembling, aligning, and optically joining at least two elements made from transparent material, the improved flow cell has a seamless internal flow channel of preferably non-circular cross-section in a cylindrical first element through which prepared samples can be metered and an independent second element having an external envelope suited to acquisition of optical parameters from formed bodies in such suspensions, the second element being conforming and alignable to the first element so that non-axisymmetric refractive effects on optical characterizing parameters of formed bodies passing through the flow channel in the first element may be minimized before the two elements are optically joined and fixed in working spatial relationship. 1. A method for making a transparent compound optical flow cell of the type used to characterize formed bodies passing through the flow cell , the optical flow cell having formed therein a rectilinear internal flow channel , the method comprising the steps of:providing a cylindrical monolithic preform comprising a thick-wall glass tube having an axially-extending channel therethrough and a transition temperature, the channel comprising a substantially uniform original cross-section of a desired shape;heating the preform to a predetermined temperature above the transition temperature of the glass tube;axially drawing the preform at a controlled rate, for a controlled time, and at a constant angular orientation, to achieve a desired reduced cross-sectional area of the axially-extending channel;providing an optical element, the optical element comprising a conforming surface that conforms to a segment of the drawn preform, and an exterior non-cylindrical envelope of predetermined form and orientation relative to the conforming surface;assembling the optical element ...

OPTICAL PARTICLE SORTER

A process for optically sorting a plurality of particles includes: providing a particle receiver; producing particles; receiving the particles by the particle receiver; receiving a light by the particle receiver; producing a standing wave optical interference pattern in an optical interference site of the particle receiver from the light; subjecting the particles to an optical gradient force from the standing wave optical interference pattern; deflecting the particles into a plurality of deflected paths to form the sorted particles from the particles; and propagating the sorted particles from the optical interference site through the deflected paths to optically sort the particles 1. An optical particle sorter comprising: a particle entrance that receives a plurality of particles;', 'an optical entrance that receives light and that is geometrically disposed at a non-parallel angle with respect to the particle entrance;', 'a sorted particle exit opposing the particle entrance and that communicates sorted particles from an optical interference site; and', 'the optical interference site interposed between the particle entrance and the sorted particle exit;, 'a particle receiver comprising produces a first light; and', 'produces a standing wave optical interference pattern in the optical interference site of the particle receiver; and, 'a first light source in optical communication with the particle receiver and that provides the particles; and', {'b': '24', 'communicates the particles to the particle receiver at an acute angle with respect to the standing wave optical interference pattern ,'}], 'a particle source in fluid communication with the particle receiver and thatwherein the optical particle sorter sorts the particles into a plurality of sorted particles that exit the particle receiver at the sorted particle exit, andthe sorted particles propagate in a plurality of deflected paths relative to a path of propagation of the particles at the particle entrance, the ...

Automated real-time particle characterization and three-dimensional velocimetry with holographic video microscopy

An in-line holographic microscope can be used to analyze on a frame-by-frame basis a video stream to track individual colloidal particles' three-dimensional motions. The system and method can provide real time nanometer resolution, and simultaneously measure particle sizes and refractive indexes. Through a combination of applying a combination of Lorenz-Mie analysis with selected hardware and software methods, this analysis can be carried out in near real time. An efficient particle identification methodology automates initial position estimation with sufficient accuracy to enable unattended holographic tracking and characterization.

SYSTEM AND METHOD FOR IMMERSION FLOW CYTOMETRY

An immersion cytometry system () having a primary focusing optic immersed in a fluid stream () containing suspended particles (). The system includes a light source () configured to illuminate a sensing region in the fluid stream that includes a focus of the primary optic. Light scattered and/or fluoresced from suspended particles passing through the sensing region is focused by an external tube lens on an external detector. The primary optic in one embodiment is a ball lens. In some embodiments, one or more filter/beam splitters on the optical axis reflect a portion of the signal light towards corresponding detectors, each filter being configured to reflect a preselected waveband of light. 1. A flow cytometry system for detecting particles suspended in a fluid stream , the system comprising:a light source configured to direct light through a sensing region in the fluid stream;a primary focusing optic at least partially immersed in the fluid stream and positioned to receive and focus light scattered by the suspended particles when they are in the sensing region; anda tube lens positioned to receive light from the primary optic and to focus the received light onto a first photo detector, the primary optic and the tube lens defining an optical axis that is aligned with the photo detector;wherein the first photo detector is configured to generate a signal responsive to the light focused by the tube lens onto the first photo detector and to transmit the generated signal to a processing system.2. The immersion flow cytometry system of claim 1 , wherein the primary focusing optic comprises a ball lens.3. The immersion flow cytometry system of claim 2 , wherein the ball lens comprises glass.4. The immersion flow cytometry system of claim 3 , wherein the glass is a flint glass.5. The immersion flow cytometry system of claim 1 , wherein the primary focusing optic is configured to chromatically distort the light claim 1 , such that the location of the focus plane on the ...

FLOW CYTOMETER AND PARTICLE DETECTION METHOD

A flow cytometer, in which detection of light generated from a particle is less likely to be affected by change in a flow velocity of a liquid flowing in a flow cell, is provided. The flow cytometer includes: a flow cell () in which a liquid flows; a liquid sending unit () configured to send the liquid into the flow cell (); a controller () configured to obtain information related to a flow velocity of the liquid flowing in the flow cell (); a light source () configured to irradiate the liquid flowing in the flow cell () with light; and a detector () configured to detect light generated from a particle in the liquid irradiated with light. The controller () changes a liquid sending condition for the liquid sending unit (), based on the obtained information related to the flow velocity. 1. A flow cytometer comprising:a flow cell in which a liquid flows;a liquid sending unit configured to send the liquid into the flow cell;a controller configured to obtain information related to a flow velocity of the liquid flowing in the flow cell;a light source configured to irradiate the liquid flowing in the flow cell with light; anda detector configured to detect light generated from a particle in the liquid irradiated with light, whereinthe controller changes a liquid sending condition for the liquid sending unit, based on the obtained information related to the flow velocity.2. The flow cytometer according to claim 1 , wherein the controller further changes a detection condition for the detector claim 1 , based on the obtained information related to the flow velocity.3. The flow cytometer according to claim 2 , wherein the controller selectively changes the liquid sending condition for the liquid sending unit or the detection condition for the detector claim 2 , based on the obtained information related to the flow velocity.4. The flow cytometer according to claim 2 , whereinthe controllerchanges the liquid sending condition for the liquid sending unit when a difference between ...

OPTICAL MEASUREMENT APPARATUS, AND OPTICAL MEASUREMENT METHOD

An optical measurement apparatus includes a main body base, an optical base movably combined with the main body base, a measurement optical system fixed to the optical base, and an optical base moving mechanism which moves the optical base relative to the main body base. The optical base moving mechanism moves the optical base relative to the main body base between an internal measurement position and an external measurement position. A measurement object position of the measurement optical system coincides with an internal measurement object position within the main body base. The measurement object position of the measurement optical system coincides with an external measurement object position outside the main body base. 1. An optical measurement apparatus comprising:a main body base;an optical base movably combined with the main body base;a measurement optical system fixed to the optical base; andan optical base moving mechanism which moves the optical base relative to the main body base between an internal measurement position defined such that a measurement object position of the measurement optical system coincides with an internal measurement object position within the main body base and an external measurement position defined such that the measurement object position of the measurement optical system coincides with an external measurement object position outside the main body base.2. The optical measurement apparatus according to claim 1 , further comprising a sample stage which is supported by the main body base and retains a sample holder to hold a sample claim 1 , wherein the internal measurement object position corresponds to a position of the sample held by the sample holder.3. The optical measurement apparatus according to claim 2 , further comprising a sample stage moving mechanism which moves the sample stage relative to the main body base between a measurement stage position defined such that the sample held by the sample holder is located at the ...

ADVANCED BIOPHYSICAL AND BIOCHEMICAL CELLULAR MONITORING AND QUANTIFICATION USING LASER FORCE CYTOLOGY

The present invention is directed to intelligent algorithms, methodologies and computer-implemented methodologies for biophysical and biochemical cellular monitoring and quantification enabling enhanced performance and objective analysis of advanced infectivity assays including neutralization assays and adventitious agent testing using fluidic and optical force-based measurements. 1. A method for measuring cellular responses to differential stimuli using optical and/or fluidic forces , wherein the method comprises:receiving a selection of an initial samples comprising biological cells treated with varying known levels of stimuli or analyte,performing optical force-based measurements on the samples,developing a response metric (RM) to describe the cellular response to the stimuli based on one or more optical or fluidic force-based parameters.2. The method of claim 1 , wherein the response metric is used to measure the response of additional unknown samples.3. The method of claim 1 , further comprising analyzing dilutions of the sample until an accurate measurement of the infectivity is determined claim 1 , based upon having an RM that falls within the acceptable target value range.4. (canceled)5. The method of claim 1 , where the optical and fluidic forces are based on laser force cytology.6. The method of further comprising:comparing the response metric of an initial sample to a target value;selecting a second sample based on the results of the first and an algorithm governing the expected or known response;comparing the response metric of the second sample to a target value; andselecting subsequent samples in a similar manner until a sample matching the target response metric or other defined endpoint is identified.7. The method of claim 5 , wherein the optical force-based measurements utilize laser force cytology to assess parameters comprising linear velocity claim 5 , size claim 5 , perimeter claim 5 , size (area claim 5 , diameter claim 5 , volume claim 5 , etc ...

MICROPARTICLE MEASURING APPARATUS AND MICROPARTICLE MEASURING METHOD

To provide a technology of maintaining light detection accuracy at a high level irrespective of individual variations in flow rate of microparticles flowing through a flow channel. The present technology provides a microparticle measuring apparatus including: a plurality of light detection sections configured to detect, at different positions, optical information emitted from microparticles flowing through a flow channel; and a detection timing control section configured to control a detection timing of each light detection section, on the basis of a trigger signal detected at a first reference channel provided in a first light detection section, and an optical signal detected at a second reference channel provided in a second light detection section that detects optical information emitted from the microparticles, at a position different from a position of the first light detection section. 1. A microparticle measuring apparatus comprising:a plurality of light detection sections configured to detect, at different positions, optical information emitted from microparticles flowing through a flow channel; anda detection timing control section configured to control a detection timing of each light detection section, on a basis of a trigger signal detected at a first reference channel provided in a first light detection section, and an optical signal detected at a second reference channel provided in a second light detection section that detects optical information emitted from the microparticles, at a position different from a position of the first light detection section.2. The microparticle measuring apparatus according to claim 1 , whereinthe detection timing control section controls the detection timing of each light detection section in real time.3. The microparticle measuring apparatus according to claim 1 , whereinthe detection timing control section controls a detection process period of the second light detection section.4. The microparticle measuring ...

Bio Sensor and Air Cleaner Having Same

There is provided a biosensor, which may measure the concentration of indoor bioaerosols through an optical sensing method of sensing ultraviolet light scattered by bioaerosols, and an air cleaner having the same. The biosensor includes a light irradiator and an ultraviolet light sensor detecting scattered ultraviolet light, from light irradiated by the light irradiator, reflected from bioaerosols. The biosensor may measure the concentration of indoor bioaerosols through the optical sensing method in real time, using a certain wavelength of ultraviolet light scattered by bioaerosols, and may allow the air cleaner to be operated under proper conditions based on a measured concentration of bioaerosols and a measured concentration of dust particles.

White Blood Cell Analysis System and Method

Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WB Cs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis.

Basophil Analysis System and Method

Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.

Nucleated Red Blood Cell Analysis System and Method

Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs. 1. A hematology analyzer for conducting a nucleated red blood cell (nRBC) analysis on a blood sample that contains a plurality of nRBCs , the analyzer comprising:an excitation source positioned to excite particles within the blood sample;a plurality of detectors including (1) an axial light loss detector positioned to measure axial light loss from the excited blood sample, (2) an intermediate angle scatter detector positioned to measure intermediate angle scatter from the excited blood sample, (3) a side scatter detector positioned to measure 90° side scatter from the excited blood sample, and (4) a fluorescence detector positioned to measure fluorescence emitted from the excited blood sample; and (a) dilute the blood sample with a reagent that includes a red blood cell (RBC) lysing agent and a cell membrane permeable, nucleic acid binding fluorescent dye;', '(b) incubate the diluted blood sample of step (a) for an incubation period of time', '(c) deliver the incubated sample from step (b) to a flow cell in the hematology analyzer;', '(d) excite the incubated ...

Method for Hematology Analysis

A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent.

ACHROMATIC ANASTIGMATIC ANAMORPHIC OBJECTIVE

In a flow cytometer, an objective lens for focusing an input laser-radiation beam including at least four different laser-radiation wavelengths in a common plane includes only three singlet lens-elements. Two of the elements are cylindrical elements arranged as a cylindrical telescope for shaping and reducing the size of the input laser-beam. The third element is a spherical element arranged to focus the reduced size beam in the common plane. In one example, all three elements are made from the same optical material. 1. An objective lens for focusing an input beam of laser-radiation in a focal plane , comprises:first, second, and third optical elements in consecutive numerical order in a propagation direction of the beam;{'sub': 'CL1', 'the first optical element being a singlet cylindrical element having a focal length f;'}{'sub': 'CL2', 'the second optical element being a singlet cylindrical element having a focal length f;'}{'sub': 'FFL', 'the third optical element being a singlet rotationally symmetrical element having a focal length f; and'}{'sub': CL1', 'CL2', 'FFL, 'claim-text': {'br': None, 'i': f', 'f', 'G', 'f, 'sub': CL1', 'CL2', 'FFL, '−=*'}, 'wherein f, f, and fare related by an equation'}where G is between about 0.7 and about 1.4.2. The objective lens of claim 1 , wherein G is between about 0.9 and 1.1.3. The objective lens of claim 1 , wherein the third element is a spherical element.4. The objective lens of claim 1 , wherein each of the lens elements has a plane surface and a curved surface.5. The objective lens of claim 4 , wherein the curved surface of each of the optical elements is the first surface of that element in the propagation direction of the beam.6. The objective lens of claim 1 , the first claim 1 , second claim 1 , and third optical elements are made from the same material.7. The objective lens of claim 6 , wherein the optical elements are made from fused silica.8. The objective lens of claim 6 , wherein the optical elements are made ...

Monitoring device for adjusting light irradiation in particle analysis apparatus

Provided is a monitoring device having a control part for monitoring, wherein the control part for monitoring has a first data processing part, a second data processing part, and an indication value output part. The first data processing part generates, as indication values for optical axis adjustment, the first indication value showing the intensity of a light-receiving signal obtained from the particle analysis apparatus. The second data processing part generates, as indication values for gain adjustment of the light-receiving signal, the second indication value showing the intensity of a light-receiving signal obtained from the particle analysis apparatus, which is different from the aforementioned first indication value. The indication value output part outputs the first and second indication values to a display device.

Particle Detection Using Thin Lenses

An optical excitation system comprises a substrate () comprising at least one delivery means (), for delivering analytes () into at least one region of interest (), at least one radiation carrier () for directing at least one radiation beam from the at least one radiation carrier () into the at least one region of interest (). The substrate () includes a thin lens system () comprising at least a first thin lens (), for collimating radiation from the at least one region of interest () to a remote detection system (). A particle sensor and sensing system comprising the excitation system are also provided, for example a modular particle sensor and modular sensing system, wherein the optical excitation system may be single use and disposable. 1. An optical excitation system for a particle sensor , the system comprising: at least one delivery means for delivering analytes into at least one region of interest, and', 'at least one radiation carrier for carrying radiation and directing at least one radiation beam from the at least one radiation carrier into the at least one region of interest; and, '(a) a substrate comprising'}(b) a thin lens system comprising at least a first thin lens for collimating radiation from the at least one region of interest to a remote detection system.2. The optical excitation system according to claim 1 , wherein the at least a first thin lens is integrated in or on the substrate.3. The optical excitation system according to claim 1 , wherein the thin lens system comprises at least one thin film lens.4. The optical excitation system according to claim 1 , wherein the at least one delivery means comprises at least one microfluidic channel.5. A particle sensor comprising:{'claim-ref': {'@idref': 'CLM-00001', 'claim 1'}, 'an optical excitation system according to ; and'}a detection system comprising at least a first detector for detecting radiation within a predetermined range of wavelengths.6. The particle sensor according to claim 5 , wherein ...

SYSTEM AND METHOD OF LABEL-FREE CYTOMETRY BASED ON BRILLOUIN LIGHT SCATTERING

The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample. 1. A method for classifying biological cells , the method comprising:providing a container having a biological sample including the biological cells in a media;illuminating the biological sample to generate a Brillouin scattered light from within the biological cells and the media;measuring a Brillouin scattering spectrum at multiple points within each biological cell;extracting one or more metrics related to subcellular physical properties at different spatial points within the biological cells based on the measured Brillouin scattering spectrum; andclassifying the biological cells based on the subcellular physical properties at different spatial points within the biological cells.2. The method of claim 1 , wherein the one or more metrics associated with the Brillouin-scattering spectrum are selected from the group consisting of: Brillouin frequency shift claim 1 , Brillouin spectrum linewidth claim 1 , Brillouin gain or loss spectrum claim 1 , and a combination thereof.3. The method of claim 1 , wherein the physical properties of the sample are selected from the group consisting of: viscoelastic modulus claim 1 , density claim 1 , refractive index claim 1 , electrostriction claim 1 , and a combination thereof.4. The method of claim 1 , wherein the step of extracting subcellular physical properties at different spatial points within the biological cells further comprises:plotting a ...

MEASUREMENT OF SERUM LIPOPROTEINS

Although a more accurate estimate of a person's risk of cardiovascular disease can be made on the basis of the number of lipoprotein particles per unit volume in the person's blood, current methods all rely on measuring the mass of lipoprotein cholesterol per unit volume. It has been discovered that a rapid and accurate lipoprotein particle count can be obtained by photometry. A method and apparatus are provided for measuring the number of lipoprotein particles in a sample using photometry. 1. A method of measuring lipoprotein particle count in a sample from a subject , the method comprising:a. introducing into an apparatus for measuring the lipoprotein particle number, a plurality of lipoprotein fractions comprising a LDL fraction, a HDL fraction, and a VLDL fraction, wherein each of the plurality of lipoprotein fractions are separated from one another;b. obtaining a photometric measurement in at least the LDL fraction; andc. calculating a particle count for the LDL fraction that is a function of the photometric measurement,wherein separation of the HDL fraction and the VLDL fraction is maintained during steps (a) to (c).2. The method of further comprising:a. obtaining a photometric measurement in the HDL fraction; andb. calculating a particle count for the HDL fraction that is a function of the photometric measurement.3. The method of further comprising:a. obtaining a photometric measurement in at least the VLDL fraction; andb. calculating a particle count for the VLDL fraction that is a function of the photometric measurement.4. The method of further comprising:a. obtaining a photometric measurement in at least the HDL fraction;b. calculating a particle count for the HDL fraction that is a function of the photometric measurement;c. obtaining a photometric measurement in at least the VLDL fraction; andd. calculating a particle count for the VLDL fraction that is a function of the photometric measurement.5. The method of claim 1 , wherein the plurality of ...

Environmental system on module apparatus

An environmental system on a module apparatus for detecting and measuring particles entrained in an air stream. The apparatus includes a highly-integrated silicon circuit board. The circuit board has a plurality of embedded sensors, signal conditioning and processing. The plurality of sensors to detect pollution and other aspects of the physical environment. The apparatus also including a mechanical structure surrounding the top side of the circuit board. The structure forms an air pathway having an inlet and an outlet and a laser beam pathway having an emitter end and a beam line termination end. The air pathway intersects the laser beam pathway. A highly miniaturized optical particle scattering detector is located proximal to the intersection. A laser is located in the emitter end of the laser beam pathway.

ANALYSIS APPARATUS AND FOCUSING METHOD

An analysis apparatus comprises a flow cell which has a flow passage for a liquid containing a tangible component; an image pickupper which picks up images of the liquid flowing through the flow passage; an adjuster which adjusts a relative position of the flow cell with respect to the image pickupper in relation to an optical axis direction of the image pickupper and a direction in which the liquid flows through the flow passage; and a controller which determines an image pickup position of the flow cell in at least one of the optical axis direction and the direction in which the liquid flows, on the basis of a number of pieces of the tangible component in focusing states existing in the images of the liquid picked up by the image pickupper at a plurality of positions at which the relative position differs. 1. An analysis apparatus comprising:a flow cell which includes a flow passage for a liquid containing a tangible component;an image pickupper configured to pick up images of the liquid flowing through the flow passage;an adjuster configured to adjust a relative position of the flow cell with respect to the image pickupper in relation to an optical axis direction of the image pickupper and a direction in which the liquid flows through the flow passage; anda controller configured to judge focusing states of pieces of the tangible component existing in the images of the liquid picked up by the image pickupper at a plurality of positions at which the relative position differs, such that the controller configured to determine an image pickup position of the flow cell in at least one of the optical axis direction and the direction in which the liquid flows, on the basis of a number of the pieces of the tangible component judged to be in the focusing states.2. The analysis apparatus according to claim 1 , further comprising a light source for image pickup which emits light a plurality of times within an exposure time for one time of exposure performed by the image ...

FLOW CYTOMETER, PARTICLE ANALYZER, AND FLOW CYTOMETRIC METHOD

A flow cytometer comprises a flow cell configured to induce a flow of a sample containing object particles, a light source, an irradiating optical system configured to irradiate light from the light source on the flow of particles in the flow cell, a detecting part configured to detect light given off from the flow of particles which are irradiated by light. The irradiating optical system comprises a collective lens having an optical axis symmetric aspherical surface on one surface, and a cylindrical surface on the other surface. 1. A flow cytometer comprising:a flow cell configured to induce a flow of a sample containing object particles;a light source;an irradiating optical system configured to irradiate light from the light source on the flow of particles in the flow cell;a detecting part configured to detect light given off from the flow of particles which are irradiated by light;wherein the irradiating optical system comprises a collective lens having an optical axis symmetric aspherical surface on one surface, and a cylindrical surface on the other surface.2. The flow cytometer of claim 1 , wherein the collective lens is arranged so that the generating line of the cylindrical surface is parallel to the flow of the particles in the flow cell.3. The flow cytometer of claim 2 , wherein the optical axis symmetric aspherical surface is configured with a shape to condense the entering light at the focal point claim 2 ,the cylindrical surface is configured to shape the light irradiating the flow region so that the width in the direction parallel to the flow of particles in the flow cell is less than the width in the direction traversing the flow of the particles.4. The flow cytometer of claim 3 , wherein the optical axis symmetric aspherical surface is provided on the light entrance surface of the collective lens claim 3 , andthe cylindrical surface is provided on the light exit surface of the collective lens.5. The flow cytometer of claim 3 , wherein the optical ...

SYSTEMS AND METHODS FOR PANEL DESIGN IN FLOW CYTOMETRY

Embodiments of the present invention encompass systems and methods for determining detection limits for various antibody-dye conjugates for flow cytometry. Exemplary techniques involve a linear superpositioning approach of spillover-induced enlargements of normally distributed measurement errors. 1. A method of designing a probe panel for a flow cytometer , the method comprising:determining a distortion factor that quantifies spillover effect caused by emission of a first label, intended to be measured in a first channel, into a second channel;inputting a maximum expected signal of a first probe-label combination including the first label and a first probe;calculating an increase in detection limit in the second channel based on the distortion factor and the maximum expected signal of the first probe-label combination; andselecting a probe-label combination to include in the probe panel based on the calculated increase in detection limit.2. The method of claim 1 , wherein the distortion factor is an estimate of an increase in detection limit in the second channel as a function of an emission intensity of the first probe-label combination.3. (canceled)4. The method of claim 2 , wherein the increase in detection limit in the second channel is caused by an increase in a measurement error as a function of the emission intensity of the first probe-label combination.5. The method of claim 2 , wherein the distortion factor is calculated using a crosstalk index.6. The method of wherein the distortion factor is mathematically modified by a coefficient representing the coexpression pattern of antigens corresponding to the first probe-label combination and a second probe-label combination claim 1 , the second probe-label combination intended to be measured in the second channel.7. The method of further comprising:determining a distortion factor for each label in a first potential probe panel to calculate a total increase in detection limit in the second channel.8. The method ...

Analysis device

An analysis device includes an analysis unit configured to receive scattered light, transmitted light, fluorescence, or electromagnetic waves from an observed object located in a light irradiation region light-irradiated from a light source and analyze the observed object on the basis of a signal extracted on the basis of a time axis of an electrical signal output from a light-receiving unit configured to convert the received light or electromagnetic waves into the electrical signal.

Counting compartment and method for sample analysis

The invention relates to a counting chamber for a microscope, adjustable in height. The method measures a cell count at two different chamber heights and deduces the concentration of particles without an exact calibration of the absolute height, as the height difference gives a measurement of the “missing” volume and therefore an estimate of the particle concentration.

PARTICLE COUNTING APPARATUS, PARTICLE COUNTING METHOD, AND PARTICLE CONTAINING SAMPLE

A particle counting apparatus is provided that includes: a droplet discharger configured to discharge a droplet containing at least one luminescent particle capable of emitting light upon receiving light; a light irradiator configured to irradiate the droplet discharged by the droplet discharger with light; at least one light receiver configured to receive light emitted by the at least one luminescent particle irradiated with the light emitted by the light irradiator; and circuitry configured to count luminescent particles contained in the droplet based on the light received by the at least one light receiver, the circuitry being configured to measure a presence or absence of the luminescent particles contained in the droplet; and to measure a number of the luminescent particles contained in the droplet. 1. A particle counting apparatus comprising:a droplet discharger configured to discharge a droplet containing at least one luminescent particle capable of emitting light upon receiving light;a light irradiator configured to irradiate the droplet discharged by the droplet discharger with light;at least one light receiver configured to receive light emitted by the at least one luminescent particle irradiated with the light emitted by the light irradiator; and count luminescent particles contained in the droplet based on the light received by the at least one light receiver, the circuitry being configured to measure a presence or absence of the luminescent particles contained in the droplet; and', 'to measure a number of the luminescent particles contained in the droplet., 'circuitry configured to'}2. The particle counting apparatus of claim 1 , wherein the circuitry includes:a first particle measuring device being configured to measure the presence or absence of the luminescent particles contained in the droplet; anda second particle measuring device being configured to measure the number of the luminescent particles contained in the droplet.3. The particle counting ...

FLUORESCENT IMAGE ANALYZER, ANALYZING METHOD, AND PRETREATMENT EVALUATION METHOD

A fluorescence image analyzer, analyzing method, and pretreatment evaluation method capable of determining with high accuracy whether a sample is positive or negative are provided. A pretreatment part performs pretreatment including a step of labeling a target site with a fluorescent dye to prepare a sample. A fluorescence image analyzer measures and analyzes the sample. The fluorescent image analyzer includes light sources to irradiate light on the sample, imaging part to capture the fluorescent light given off from the sample irradiated by light, and processing part for processing the fluorescence image captured by the imaging part. The processing part extracts the bright spot of fluorescence generated from the fluorescent dye that labels the target site from the fluorescence image for each of a plurality of cells included in the sample, and generates information used for determining whether the sample is positive or negative based on the bright spots extracted for each of the plurality of cells. 1. A method for analyzing cells in a sample treated with a fluorescent label specifically biding to a target site of cells , comprising:capturing fluorescence images of individual cells in the sample treated with the fluorescent label; anddigitally analyzing at least some of the fluorescence images to identify at least a positive cell having a particular pattern of bright spots based on a color and a number of bright spots, wherein a positive cell is distinguishable from a negative cell according to the pattern of bright spots.2. The method of claim 1 , further comprisinggenerating information of any one selected from a group consisting of: a number of positive cells, a number of negative cells, a ratio of the number of positive cells to the number of negative cells, and a ratio of the number of negative cells to the number of positive cells.3. The method of claim 1 , further comprisinggenerating information of a population of positive cells in the sample.4. The method of ...

PARTICLE SENSOR

A particle sensor includes: a detecting area into which a gas including particles is introduced; a light-projecting element; a light-receiving element which receives scattered light of the light reflected by the particles in the gas in the detecting area; a heater which heats the gas; a reflector which directs the scattered light to the light-receiving element, wherein the reflector includes: a first ellipsoidal portion having an inner surface shape that defines a portion of a surface of revolution of a spheroid; and a first spherical portion having an inner surface shape that defines a portion of a spherical surface of a sphere, wherein the first ellipsoidal portion has one focus of the spheroid located in the detecting area and the other focus of the spheroid located at or proximate to the light-receiving element, and the first spherical portion has a center of the sphere located in the detecting area. 1. A particle sensor comprising:a detecting area into which a gas including particles is introduced;a light-projecting element which emits light to the gas in the detecting area;a light-receiving element which receives scattered light of the light reflected by the particles in the gas in the detecting area;a heater which heats the gas;a reflector which reflects and directs the scattered light to the light-receiving element, wherein: a first ellipsoidal portion at least a portion of which is in an area between the detecting area and the light-receiving element, the first ellipsoidal portion having an inner surface shape that defines a portion of a surface of revolution of a spheroid; and', 'a first spherical portion in an area on a side opposite a light-receiving element side relative to the detecting area, the first spherical portion having an inner surface shape that defines a portion of a spherical surface of a sphere,, 'the reflector includesthe first ellipsoidal portion has a first focus, which is one of two foci of an ellipse of the spheroid of the first ...

Microparticle measuring apparatus

A microparticle measuring apparatus for highly accurately detecting the position of a microparticle flowing through a flow channel includes a light irradiation unit for irradiating a microparticle flowing through a flow channel with light, and a scattered light detection unit for detecting scattered light from the microparticle, including an objective lens for collecting light from the microparticle, a light splitting element for dividing the scattered light from the light collected by the objective lens, into first and second scattered light, a first scattered light detector for receiving an S-polarized light component, and an astigmatic element disposed between the light splitting element and the first scattered light detector, and making the first scattered light astigmatic. A relationship between a length L from a rear principal point of the objective lens to a front principal point of the astigmatic element, and a focal length f of the astigmatic element satisfies the following formula I. 1.5 f≦L≦ 2.5 f   ( I )

FAST THERMO-OPTICAL PARTICLE CHARACTERISATION

The present invention relates to a method and an apparatus for a fast thermo-optical characterisation of particles. In particular, the present invention relates to a method and a device to measure the stability of (bio)molecules, the interaction of molecules, in particular biomolecules, with, e.g. further (bio)molecules, particularly modified (bio)molecules, particles, beads, and/or the determination of the length/size (e.g. hydrodynamic radius) of individual (bio)molecules, particles, beads and/or the determination of length/size (e.g. hydrodynamic radius). 1. Method to measure thermo-optically characteristics of particles in a solution with the steps of:providing a sample probe with marked particles in a solution;exciting fluorescently said marked particles and firstly detecting fluorescence of said excited particles;irradiating a laser light beam into the solution to obtain a spatial temperature distribution in the solution around the irradiated laser light beam;detecting secondly a fluorescence of the particles in the solution at a predetermined time after irradiation of the laser into the solution has been started, andcharacterizing the particles based on said two detections.2. The method according claim 1 , wherein the predetermined time is within the range of 1 ms to 250 ms3. The method according to or claim 1 , wherein the detection time is in the range of 1 ms to 50 ms.4. The method according to claim 1 , or claim 1 , wherein the laser beam is defocused such that a temperature gradient within the temperature distribution is in the range of from 0.0 to 2K/μm claim 1 , preferably from 0.0 to 5K/μm.5. The method according to claim 4 , wherein the laser beam is irradiated through an optical element into the solution.6. The method according to claim 4 , wherein the optical element is a single lens.7. The method according to any of the preceding claims claim 4 , further comprising the step of measuring the temperature distribution in said solution around the ...

Condensation Particle Counter Comprising Saturation Unit and Downstream Condensation Unit

A condensation particle counter comprising a saturation unit () and a downstream condensation unit (), through which at least one channel () for an aerosol flow passes between an inlet () and an outlet () that leads to a counting unit (). The saturation unit () and the condensation unit () comprise a shell sleeve () having an inner shell wall () that is penetrated by a core (), the core wall () and the inner shell wall () delimiting a channel () formed therebetween. 1. A condensation particle counter comprising a saturation unit and a downstream condensation unit , through which at least one channel for an aerosol flow passes between an inlet and an outlet that leads to a counting unit , wherein the saturation unit and the condensation unit comprise a shell sleeve having an inner shell wall that is penetrated by a core , the core wall and the inner shell wall delimiting the channel formed therebetween.2. The condensation particle counter according to claim 1 , wherein the channel has an annular cross-section with a core diameter and a shell wall diameter.3. The condensation particle counter according to claim 2 , wherein the annular cross-section of the channel is constant along the longitudinal direction of the channel.4. The condensation particle counter according to claim 1 , wherein the channel is arranged so as to be substantially vertical when the condensation particle counter is used as intended.5. The condensation particle counter according to claim 1 , wherein a condensate trap is provided between the saturation unit and the condensation unit.6. The condensation particle counter according to claim 5 , wherein the condensate trap is configured as a filtration element.7. The condensation particle counter according claim 1 , wherein the channel runs in a straight line.8. The condensation particle counter according to claim 1 , wherein the channel has a gap width in the range of about 2.3 to about 3.0 mm and a length L in the range of about 62 to about 82 mm.9. ...

OPTICS SYSTEM FOR A FLOW CYTOMETER

A flow cytometer includes a flow nozzle, a light source, an optics system, and a sensor analyzer. The flow nozzle provides a fluid along a flow path. The light source generates a light beam that illuminates the fluid. The optics system collects light rays that are radiated from the light beam by the fluid and passes or blocks the light rays based at least in part on the radiation angles associated with the light rays. 1. An optics system of a flow cytometer , the optics system comprising:a collection optics assembly arranged and configured to be positioned adjacent a fluid flow path and aligned with a path of a beam from a light source to collect light rays radiated from the beam by the fluid, or by particles in the fluid, in the fluid flow path;a collimator arranged to receive the light rays from the collection optics assembly, wherein the collimator directs the light rays through a focus insensitive region in which positions of the light rays are independent of fluctuations in the position of the fluid flow path with respect to the collection optics assembly; anda filter mask positioned at the focus insensitive region to selectively filter the light rays based on radiation angles associated with the light rays.2. The optics system of claim 1 , further comprising a re-imager optically arranged between the collection optics assembly and the collimator.3. The optics system of claim 1 , wherein the light source is a laser.4. The optics system of claim 1 , wherein the light rays radiated from the beam by the fluid claim 1 , or by particles in the fluid claim 1 , are forward-scattered.5. The optics system of claim 1 , wherein the light rays further comprise fluorescent light.6. The optics system of claim 1 , wherein the filter mask selectively blocks light rays having certain radiation angles and selectively passes light rays having other radiation angles.7. The optics system of claim 1 , further comprising a stray light control structure positioned at a focal point of ...

DETECTION OF PARTICLE CHARACTERISTICS

A method of detecting particles in an air flow is described. The method includes receiving a signal indicative of light intensity scattered from the air flow at a plurality of wavelengths and processing the signal indicative of the intensity of received light at each of the wavelengths and a corresponding wavelength dependent parameter to generate an output signal indicative of at least one characteristic of particles in the air flow. A particle detection system is also described. 1. A particle detection system for including:a detection chamber adapted to receive an air sample;first particle detection means including a first light source for illuminating a first volume of the air sample at at least a first wavelength, and a first light receiver having a field of view intersecting with the first volume for receiving light scattered from the detection chamber and outputting a first signal indicative of the scattered received light;second particle detection means including a second light source for illuminating a second volume of the air sample at at least a second wavelength, and a second light receiver having a field of view intersecting with the second volume for receiving light scattered from the detection chamber and outputting a second signal indicative of the scattered received light;light source activation means adapted to selectively activate the first light source in a first time period and the second light source in a second time period; andprocessing means adapted to receive a first signal from the first light receiver and a second signal from the second light receiver corresponding to the first time period and process the received signals to generate a first output corresponding to the first time period corrected for background light; and to receive a first signal from the first light receiver and a second signal from the second light receiver corresponding to the second time period and process the received signals to generate a second output corresponding ...

SYSTEMS AND METHODS FOR PARTICLE TRACKING USING SPATIOTEMPORAL OFFSET LIGHT BEAMS

Systems and methods for particle tracking using spatiotemporal offset light beams. In exemplary embodiments, the optical systems and methods can be used with conventional two-photon microscopy equipment to perform high speed, high precision, and deep tissue three-dimensional single-particle tracking. Exemplary embodiments can be configured for single-molecule studies of biological diffusion and transport processes. 1. A system comprising:a light source;a plurality of beam splitters configured to separate an input light beam emitted from the light source into a plurality of excitation light beams;a first optical component configured to direct one or more of the excitation light beams in a first lateral dimension;a second optical component configured to direct one or more of the excitation light beams in a second lateral dimension;a third optical component configured to adjust the collimation of one or more of the excitation light beams; and 'the first pair of excitation beams and the second pair of excitation beams comprise spatiotemporally multiplexed light beams.', 'an output beam splitter configured to transmit a first pair of excitation beams and configured to reflect a second pair of excitation beams, wherein2. The system of wherein:the first pair of excitation beams transmitted by the output beam splitter comprises a first excitation beam and a second excitation beam; andthe first excitation beam is temporally offset between 1 and 20 nanoseconds from the second excitation beam.3. The system of wherein:the second pair of excitation beams transmitted by the output beam splitter comprises a third excitation beam and a fourth excitation beam; andthe third excitation beam is temporally offset between 1 and 20 nanoseconds from the fourth excitation beam.4. The system of wherein the third excitation beam is temporally offset between 1 and 20 nanoseconds from the second excitation beam.5. The system of wherein:the first pair of excitation beams transmitted by the ...

OPTO-MECHANICAL SYSTEM AND METHOD HAVING CHAOS INDUCED STOCHASTIC RESONANCE AND OPTO-MECHANICALLY MEDIATED CHAOS TRANSFER

An a system and method for chaos transfer between multiple detuned signals in a resonator mediated by chaotic mechanical oscillation induced stochastic resonance where at least one signal is strong and where at least one signal is weak and where the strong and weak signal follow the same route, from periodic oscillations to quasi-periodic and finally to chaotic oscillations, as the strong signal power is increased. 1. A method for chaos transfer between multiple signals comprising:transmitting multiple detuned signals in an optical micro cavity resonator with optomechanically induced oscillation where at least one signal is stronger than and detuned with respect to at least one other signal; andincreasing the power of the at least one signal whereby as the power is increased the at least one signal and the at least one other signal follow the same route, from periodic oscillations to quasi-periodic and finally to chaotic oscillations.2. The method for chaos transfer as recited in claim 1 , where the at least one signal is an optical field pump exciting mechanical oscillations in the resonator and the at least one other signal is an optical field probe and where chaos transfer from the pump to the probe is mediated by the mechanical motion of the resonator.3. The method for chaos transfer as recited in claim 2 , where the at least one signal is a pump laser and the at least one other signal is a probe laser4. The method for chaos transfer as recited in claim 3 , where optomechanically induced chaos modulate the at least one other signal at a frequency of the mechanical oscillation.5. The method for chaos transfer as recited in claim 4 , where transmitting multiple signals in an optical micro cavity resonator includes coupling the at least one signal and the at least one other signal into and out of the micro cavity resonator with one or more of a waveguide claim 4 , an optical fiber and free-space claim 4 , and separating the at least one signal from the at least one ...

Systems, Methods, and Apparatuses For Optical Systems In Flow Cytometers

The present set of embodiments relate to a system, method, and apparatus for an optical configuration in a flow cytometer that allows for independent adjustment of focusing for each light source. Such systems, methods, and apparatuses require a final focusing element to be moved near the beginning of the optical train and for each optical element coming after the final focusing element to be configured to accommodate converging light beams while minimizing the introduction of aberrations into those beams.

Method and Apparatus of Filtering Light Using a Spectrometer Enhanced with Additional Spectral Filters with Optical Analysis of Fluorescence and Scattered Light from Particles Suspended in a Liquid Medium Using Confocal and Non Confocal Illumination and Imaging

A system for filtering light using a spectrometer enhanced with spectral filters using an array of independent photodetectors to measure the fluorescent or scattered light signal. A system comprising a light source, an illuminated sample, a light spectrum device, a collimator lens, a plurality of spectral filters each having a varying and selected light transmission spectrum and a plurality of photodetectors wherein each photodetector is oriented to a spectral filter. A scanning cytometer for measuring fluorescence and light scattering from an illuminated portion of the sample comprising a first light source, a scanner scanning in two axes, a fluorescence detector, an objective lens, an optically translucent medium through which a sample may be illuminated and a confocal apparatus positioned distally from the light source and sample and through which light signals from the sample are transmitted to a fluorescence detector.

SPECTRAL MICROSCOPE

In one implementation, a spectral microscope may comprise a substrate with a planar lens, the planar lens including a phase profile including an axial focus and an oblique focus, a light source to excite a signal of a particle among a plurality of particles, and a detector to receive light generated from the light source from the axial focus of the planar lens and a spectral color component of the excited signal of the particle from the oblique focus of the planar lens. 1. A spectral microscope , comprising:a substrate with a planar lens patterned on the substrate, the planar lens including a phase profile including an axial focus and an oblique focus;a chip having a channel, wherein the chip is adjacent to the substrate; and light generated from a light source through the chip and the substrate from the axial focus of the planar lens; and', 'a spectral color component of an excited signal of a particle located in the channel through the chip and the substrate from the oblique focus of the planar lens., 'a detector to receive2. The spectral microscope of claim 1 , wherein the planar lens is a diffractive lens.3. The spectral microscope of claim 1 , wherein the detector is positioned above the substrate.4. The spectral microscope of claim 3 , wherein the light source is positioned below the chip to excite the signal of the particle such that the detector positioned above the substrate is to receive the light from the axial focus of the planar lens and the spectral color component of the particle from the oblique focus of the planar lens.5. The spectral microscope of claim 1 , wherein the planar lens is a partially reflective lens to reflect the spectral color component to the detector.6. The spectral microscope of claim 1 , wherein the detector is positioned in the chip and located adjacent to the channel.7. The spectral microscope of claim 6 , wherein the light source is positioned above the chip to excite the signal of the particle such that the detector positioned ...

OPTICAL PARTICLE DETECTOR

A particle detector including at least one channel intended to receive at least one fluid comprising particles and configured to receive at least one light beam emitted by a light source. The particle detector further including at least one photodetector network configured such that at least some photodetectors receive light beams emitted by the source and scattered by the particles present in the channel. The detector further comprises at least one optical system, each optical system s associated with a photodetector network and has at least one image focal plane and an optical axis. The detector is configured such that the image focal plane of the optical system is optically coupled to the photodetector network. 1. A particle detector comprising:at least one channel configured to receive at least one fluid comprising particles and configured to receive at least one light beam emitted by a light source;at least one photodetector network configured such that at least some of the photodetectors receive light beams emitted by the light source and scattered by the particles present in the channel;wherein:the detector further comprises at least one optical system configured to be passed through by one portion of the light beams after the scattering thereof by the particles and before the light beams are received by the photodetectors,each optical system is associated with a photodetector network and has an image focal plane,the detector is configured such that said image focal plane of the optical system is optically coupled to the photodetector network such that all the light beams scattered by the particles along parallel directions before passing through the optical system reach, after passing through the optical system, one same point of the photodetector network associated with the optical system,the at least one optical system s convergent so as to make the light beams coming from the light source and not scattered by the particles converge towards an image focus ...

DYNAMIC RANGE EXTENSION SYSTEMS AND METHODS FOR PARTICLE ANALYSIS IN BLOOD SAMPLES

For analyzing a sample containing particles of at least two categories, such as a sample containing blood cells, a particle counter subject to a detection limit is coupled with an analyzer capable of discerning particle number ratios, such as a visual analyzer, and a processor. A first category of particles can be present beyond detection range limits while a second category of particles is present within respective detection range limits. The concentration of the second category of particles is determined by the particle counter. A ratio of counts of the first category to the second category is determined on the analyzer. The concentration of particles in the first category is calculated on the processor based on the ratio and the count or concentration of particles in the second category. 1. A method for measuring a quantity of a first cell type in a blood fluid sample , the sample including a second cell type , the method comprising:determining a population of the second cell type in a first volume of the sample by flowing the first volume through a hematology cell counter;acquiring images of a first number of the first type cells and a second number of the second cell types by injecting a second volume of the sample into a sheath fluid flowing within a flowcell so as to provide a sample stream having a thickness and a width greater than the thickness, the acquired images being acquired along an image path traversing the thickness of the sample stream;determining a ratio of the first number of the first cell type to the second number of the second cell types using the acquired images; andcalculating a cell quantity measure of the first cell type in the sample using the ratio and the population of the second cell type.2. The method of claim 1 , wherein the cell quantity measure comprises a cell concentration for the first cell type in the blood fluid sample.3. The method of claim 1 , wherein the cell quantity measure comprises a cell count for the first cell type ...

OPTICAL PARTICLE SENSOR AND SENSING METHOD

The invention provides an optical particle sensor which uses light sources of different first and second wavelengths. A first light source is used to detect light scattering and also to determine when a particle reaches a target positional area. In response to the particle being determined to reach the target positional area, a second light source is operated to provide a pulse of light, and light emitted from the particle in response to the pulse of light is detected by the same detector. 1. An optical particle sensor , comprising:an optical arrangement comprising at least first and second light sources of different first and second wavelengths;an optical detector for detecting light emitted from particles to be sensed or light from the light sources scattered by particles to be sensed; anda controller for controlling the operation of the first and second light sources, operate the first light source, and detect light scattering using the optical detector;', 'determine when a particle reaches a target positional area based analyzing the detected scattered light;', 'in response the particle being determined to reach the target positional area, operate the second light source with a drive signal which is at least 2 times the maximum permitted continuous drive signal to provide a pulse of light with a duty cycle of less than 20%; and', 'detect light emitted from the particle in response to the pulse of light using the same optical detector., 'wherein the controller is adapted to2. A sensor as claimed in claim 1 , wherein the optical detector comprises a single optical sensor for detecting the first and second wavelengths claim 1 , wherein the optical sensor has greater sensitivity to the first wavelength than to the second wavelength claim 1 , such as a maximum sensitivity at or adjacent the first wavelength.3. A sensor as claimed in claim 2 , further comprising a background level compensation circuit for reducing the response time for the optical detector to adapt to ...

TUNABLE LIDAR FOR SIMULTANEOUS RANGING AND ENVIRONMENTAL MONITORING

In one embodiment, a method includes emitting, by an automotive vehicle, one or more light beams from a LiDAR system toward an environment surrounding the automotive vehicle, receiving one or more return light beams at the LiDAR system of the automotive vehicle, detecting one or more objects located in the environment surrounding the automotive vehicle based on the return light beams, and simultaneously measuring one or more air characteristics of the environment surrounding the automotive vehicle. The simultaneous detecting of the one or more objects and measuring of the one or more air characteristics may be performed by the LiDAR system. The method may further include sending, by the automotive vehicle, location information to a server associated with vehicle navigation, and sending information of the detected objects and measured air characteristics to the server associated with vehicle navigation, the server configured to update a vehicle-navigation map based on the information. 1. A method comprising:emitting, by an automotive vehicle, one or more light beams from a LiDAR system toward an environment surrounding the automotive vehicle;receiving one or more return light beams at the LiDAR system of the automotive vehicle;detecting one or more objects located in the environment surrounding the automotive vehicle based on the return light beams; andsimultaneously measuring one or more air characteristics of the environment surrounding the automotive vehicle.2. The method of claim 2 , wherein the simultaneous detecting of the one or more objects and measuring of the one or more air characteristics is performed by the LiDAR system.3. The method of claim 1 , wherein the one or more air characteristics measured comprises characteristics of one or more trace gases or characteristics of one or more aerosol particles in the air surrounding the automotive vehicle.4. The method of claim 3 , wherein the measuring of the characteristics of the trace gases comprises ...

SYSTEM AND METHOD FOR CONTROLLING DROPLET DISPENSING

Systems and methods for controlling volumes of droplets is provided herein. In some cases, the systems comprise: a plurality of first light sources, a second light source, wherein a first light beam emitted a first light source of said plurality of first light sources is configured to intersect with a second light beam emitted from said second light source at an intersection area, wherein said system is configured to measure a characteristic of said droplet as it passes through said intersection area. Systems and methods for measuring volumes of droplets and for distinguishing between bubbles and droplets are also provided. 1. A system for measuring a droplet , said system comprising:(a) a plurality of first light sources; and(b) a second light source,wherein a first light beam emitted a first light source of said plurality of first light sources is configured to intersect with a second light beam emitted from said second light source at an intersection area,wherein said system is configured to measure a characteristic of said droplet as it passes through said intersection area.2. The system of claim 1 , further comprising: (c) a detector configured to detect a level of light from said first light beam.3. The system of or claim 1 , further comprising: (d) a detector configured to detect a level of light from said second light beam.4. The system of any one of - claim 1 , wherein said plurality of first light sources are arranged in an array.5. The system of any one of - claim 1 , wherein (b) further comprises a plurality of second light sources.6. The system of claim 5 , wherein said plurality of second light sources are arranged in an array.7. The system of any one of - claim 5 , wherein each first light source of said plurality of first light sources are equidistant to each other.8. The system of or claim 5 , wherein each second light source of said plurality of second light sources are equidistant to each other.9. The system of any one of - claim 5 , wherein said ...

Particle Detector

A particle detector, having a housing defining a chamber; an air stream injector, producing an airstream in said chamber from air taken from outside said chamber; a light source, producing a light beam that crosses the air stream and wherein said light beam is shaped so that a transverse extent of said light beam has a uniform intensity over said transverse extent of said air stream. Also, a photon detection assembly, including an optical train of lenses, is positioned to accept light from said light beam, emitted by the particles, and to focus this light onto a a photon detector. A particle detection assembly detects the particles, responsive the photon detection assembly. Finally, a particle size estimation assembly estimates size for each detected particle, based on number of photons detected by said photon detection assembly from said particle, as it crosses said light beam. 1. A particle detector , comprising:a. a housing defining a chamber;b. an air stream injection assembly, producing an airstream in said chamber from air taken from outside said chamber, and having a transverse extent;c. a light source, producing a light beam that intersects with and crosses said air stream at an angle, thereby forming an intersection, and wherein said light beam is sized and shaped so that a transverse extent of said light beam has a uniform intensity over said transverse extent of said air stream at said intersection;d. a photon detection assembly, including an optical train of lenses, configured and positioned to accept an amount of light from said light beam, emitted by particles in said air stream, and to focus said emitted light onto a detection surface of a photon detector;e. a particle detection assembly, detecting particles in said air stream in response to input from said photon detection assembly; andf. a particle size estimation assembly, responsive to said photon detection assembly, which estimates size for each particle detected, based on number of photons ...

BLOOD ANALYZER AND ANALYSIS METHOD

A blood analyzer and a blood analysis method thereof are provided. The blood analyzer includes a test sample preparation apparatus, a flow chamber, a measurement apparatus and a data processor. The method adopts a white blood cell measurement channel to detect a test blood sample subjected to hemolysis and fluorescence staining and utilizes data in a blood ghost region to identify reticulocyte particles, thereby realizing differentiation of reticulocytes and large platelets and realizing finer classification and count of reticulocytes without separately designing an optical detection channel in the blood analyzer. A computer readable storage medium is also disclosed, in which a program is stored, and the program can be executed by the data processor to implement the above method. 137-. (canceled)38. A blood analyzer , comprising:a test sample preparation apparatus for preparing a test sample for measurement, wherein the test sample preparation apparatus at least comprises a reaction cell, and the reaction cell is configured for providing a place for mixing and incubation of a test blood sample, a fluorescence staining agent and a hemolytic agent, and the mixing and incubation causes red blood cells in the test blood sample to be lysed and causes cell particles in the sample to be stained;a flow chamber configured for providing an area for the cell particles in the test sample to pass through one by one and to be irradiated with light;a measurement apparatus comprising a light source and an optical detection device, wherein the light source is configured for emitting a light beam to irradiate the flow chamber, the optical detection device is configured for receiving scattered light and fluorescent light generated by the cell particles under the irradiation of light and outputting scattered light information and fluorescent light information, and the scattered light information at least comprises first angle scattered light information which is used for reflecting ...

Hematology systems and methods

Aspects and embodiments of the instant disclosure provide a particle and/or intracellular organelle alignment agent for a particle analyzer used to analyze particles contained in a sample. An exemplary particle and/or intracellular organelle alignment agent includes an aqueous solution, a viscosity modifier, and/or a buffer. Embodiments also encompass systems, compositions, and methods for analyzing a sample containing particles. Particles such as blood cells can be categorized and counted by a digital image processor. A digital microscope camera can be directed, for example using certain focusing techniques, into a flowcell defining a symmetrically narrowing flowpath in which the sample stream flows in a ribbon flattened by flow and viscosity parameters between layers of sheath fluid. Blood cell images can be collected and analyzed using dynamic range extension processes and systems.

BLOOD ANALYZING METHOD AND BLOOD ANALYZER

A blood analyzing method comprises irradiating light on a measurement sample prepared using fluorescent dye so as to produce a difference in fluorescence intensity between reticulocytes and mature red blood cells in a blood sample, detecting scattered light and fluorescent light given off from blood cells in the measurement sample, and obtaining fluorescence data related to the fluorescent light and scattered light data related to the scattered light for each blood cell, determining the possibility of the presence of parasites in the blood sample based on the distribution conditions of blood cells in the reticulocyte distribution range based on the scattered light data and the fluorescent light data. 1. A blood analyzing method , comprising:irradiating light on a measurement sample prepared using fluorescent dye so as to produce a difference in fluorescence intensity between reticulocytes and mature red blood cells in a blood sample;detecting scattered light and fluorescent light given off from blood cells in the measurement sample, and obtaining fluorescence data related to the fluorescent light and scattered light data related to the scattered light for each blood cell;{'i': 'plasmodium falciparum', 'determining the possibility of the presence of parasites in the blood sample based on the distribution conditions of blood cells in the reticulocyte distribution range based on the scattered light data and the fluorescent light data.'}2. The blood analyzing method of claim 1 , wherein the measurement sample is prepared without lysing the red blood cells.3plasmodium falciparum. The blood analyzing method of claim 1 , wherein determining the possibility of the presence of parasites in the blood sample is based on the proportion of blood cells included in the range of low fluorescence intensity in the reticulocyte distribution range.4. The blood analyzing method of claim 1 , wherein blood cells in the measurement sample are classified as mature red blood cells claim 1 , ...

Detection And Quantification Of Acetylamantadine In Urine Samples

A method for quantifying acetylamantadine in a urine sample comprises eluting acetylamantadine from the urine sample using solid phase extraction and quantifying the acetylamantadine eluted from the urine sample using Raman spectroscopy.

APPARATUS FOR DISCRIMINATING BACTERIA TYPES USING OPTICAL SCATTERING PATTERNS

An apparatus for discriminating bacteria types using optical scattering patterns is disclosed. The apparatus includes an optical fiber for transferring light emitted from a light source, a lens for controlling a width of the light received from the optical fiber, a linear polarizer for transmitting the light passing through the lens and a bacterial colony, and a capturing unit for capturing an optical scattering pattern of the light transmitted through the linear polarizer. 1. An apparatus for discriminating bacteria types using optical scattering patterns , the apparatus comprising:an optical fiber for transferring light emitted from a light source;a lens for controlling a width of the light received from the optical fiber;a linear polarizer for transmitting the light passing through the lens and a bacterial colony; anda capturing unit for capturing an optical scattering pattern of the light transmitted through the linear polarizer.2. The apparatus according to claim 1 , wherein a polarization controller is provided at the optical fiber claim 1 , for polarizing the light guided through the optical fiber.3. The apparatus according to claim 1 , further comprising an x-y axis stage for disposing the bacterial colony thereon claim 1 , and controlling a horizontal position of the disposed bacterial colony.4. The apparatus according to claim 1 , further comprising a z axis stage combined with the capturing unit claim 1 , for controlling a vertical distance between the capturing unit and the bacterial colony.5. The apparatus according to claim 1 , wherein the width of the light passing through the lens is controlled to 100 μm to 1 mm.6. The apparatus according to claim 2 , wherein the polarization controller includes a ½ wavelength plate or a ¼ wavelength plate. This application claims the benefit of Korean Patent Application No. 2015-0109317, filed on Aug. 3, 2015, which is hereby incorporated by reference as if fully set forth herein.Field of the InventionThe present ...

FLOATING PARTICLE DETECTION DEVICE

A floating particle detection device is capable of accurately identifying the type of a floating particle while achieving simplification of a configuration of the device, the device includes: a laser light irradiator () that includes a laser light emitting element () and a back-monitor-use light receiving element (); a scattered light receiver () that selectively receives light of a predetermined polarization component among scattered light generated when a floating particle () is irradiated and that generates a second detection signal; and an identification processor () that identifies the type of the floating particle on the basis of a first detection signal and the second detection signal. Incident light entering the back-monitor-use light receiving element () includes: a back-monitor-use laser beam (L); and backscattered light (Lbs) travelling toward the laser light irradiator () among the scattered light (Ls). 1. A floating particle detection device comprising:a laser light irradiator that includes a laser light emitting element including a front-side edge surface that emits an irradiation laser beam with which a detection-target region where floating particles are present is irradiated and a back-side edge surface that emits a back-monitor-use laser beam which travels in a direction opposite to a travel direction of the irradiation laser beam, and a back-monitor-use light receiving element disposed in a position where the back-monitor-use laser beam is incident, the back-monitor-use light receiving element generating a first detection signal according to an amount of incident light;a scattered light receiver that selectively receives light of a predetermined polarization component among scattered light of the irradiation laser beam, the scattered light being generated when a floating particle is irradiated, thereby generating a second detection signal; andan identification processor that identifies a type of the floating particle on a basis of the first ...

PARTICLE COUNTER

An irradiation optical system irradiates a fluid flowing in a flow passage 2with one light among a plurality of lights obtained by branching light from a light source and forms the detection area. A detection optical system makes scattered light with a different direction from an optical axis of the irradiation optical system enter a beam splitter among the scattered lights from particles contained in the fluid in this detection area. Meanwhile, a beam expander makes another light among the plurality of lights enter the beam splitter as reference light. A detector receives an interference light, by the scattered light and the reference light, obtained by the beam splitter by light receiving elements and generates a detection signal corresponding to the interference light. A counting unit counts the particles based on this detection signal. 1. A particle counter comprising:a light source that emits light;a light superimposition unit configured to superimpose two lights in a space;an irradiation optical system configured to irradiate a fluid flowing in a flow passage with one light among a plurality of lights obtained by branching the light from the light source to form a detection area;a detection optical system configured to make a scattered light in a direction different from an optical axis of the irradiation optical system, among scattered lights from particles contained in the fluid in the detection area, enter the light superimposition unit;a reference optical system configured to make another one light among the plurality of lights enter the light superimposition unit as a reference light;a detector configured to receive an interference light by the scattered light and the reference light at a light receiving element, the interference light being obtained by the light superimposition unit, the detector being configured to generate a detection signal corresponding to the interference light; anda counting unit configured to count the particles based on the ...

FLOW CYTOMETRY USING HYDRODYNAMICALLY PLANAR FLOW

According to various aspects, a flow system for transporting microparticulate samples in a hydrodynamically planar flow in a selected flow direction includes a flow chamber extending in the flow direction, having first and second apertures on opposed surfaces of the flow chamber. A sheath-fluid channel has first and second branches to carry the sheath fluid into the flow chamber through the first aperture and having orientations separated by less than about 15° at the first aperture; and third and fourth branches to carry the sheath fluid through the second aperture and having orientations separated by less than about 15° at the second aperture. In some examples, guide channels extend from the apertures substantially perpendicular to the flow chamber at the apertures, and sheath-fluid channel supply sheath fluid to the guide channels. Flow systems can be used in image flow cytometers for observing microparticulate samples, e.g., using scanning irradiation. 1. A flow system for transporting microparticulate samples in a hydrodynamically planar flow in a flow direction , the flow system comprising:a flow chamber extending in the flow direction, the flow chamber including first and second apertures through opposed walls of the flow chamber, the flow chamber configured to receive a carrier fluid including the microparticulate samples at a location upstream of the first and second apertures; and first and second branches, each of the first and second branches configured to carry the sheath fluid into the flow chamber through the first aperture and having a respective longitudinal axes, wherein the respective longitudinal axes have a relative angle between them of less than about 15° at the first aperture; and', 'third and fourth branches, each of the third and fourth branches configured to carry the sheath fluid into the flow chamber through the second aperture and having respective longitudinal axes, wherein the respective longitudinal axes have a relative angle between ...

PARTICLE STANDARDS FOR REFLECTED LIGHT SCATTER MEASUREMENTS FROM DEGENERATE PARTICLE FOCI

A method of selecting a type of particle for use in standardisation and/or calibration of a flow cytometer. The method includes determining the location of two or more particle focal points of particles flowing through a cross section of a channel in the flow cytometer; for each type of particle, determining for each particle focal point, for a beam of light directed at a type of particle at said particle focal point from a first direction, the total intensity of light scattered along a second direction; determining the difference between the highest and lowest determined light intensities of the light intensities determined at the two or more particle focal points; and selecting a type of particle for which the difference between the highest and lowest determined light intensities at the two or more particle focal points is below a predetermined threshold. 1. A method of selecting a type of particle for use in standardization and/or calibration of a flow cytometer , the method comprising:(a) determining a location of two or more particle focal points of particles flowing through a cross section of a channel in the flow cytometer; (b1) for each particle focal point of the two or more particle focal points, determining, for a beam of light directed at a particle of said type at said particle focal point from a first direction, a total intensity of light scattered along a second direction, the second direction lying within 90 degrees of the first direction; and', '(b2) determining, for the type of particle, the difference between the highest and lowest determined light intensities of the light intensities determined at the two or more particle focal points; and, '(b) for each type of particle of two or more different types of particles(c) selecting a type of particle for which the difference between the highest and lowest determined light intensities of the light intensities determined at the two or more particle focal points is below a predetermined threshold.2. The ...

PHOTON COUNTING AND MULTI-SPOT SPECTROSCOPY

An example system can include a support and two or more sensor elements mounted to the support. Each sensor element can be electrically connected to a common electrical node and may include: a respective quench resistor connected to a respective internal node; and a respective photodiode (PD) connected to the respective internal node; a differentiating element fed by at least one of the photodiodes; a first readout electrode fed by the common electrical node; and a second readout electrode fed by the differentiating element. The common electrical node may be connected to at least one of the quench resistors or at least one of the photodiodes. 142-. (canceled)43. A silicon photomultiplier (SiPM) system , comprising:{'claim-text': ['a plurality of photosensors, each photosensor including a quench resistor coupled in series with a Geiger-mode avalanche photodiode (APD),', 'a first electrode configured to provide an output signal of the SiPM array, the output signal comprising an amalgamation of the plurality of APD signals;', 'a second electrode configured to provide a biasing voltage to each of the plurality of photosensors;'], '#text': 'an SiPM array, comprising:'}a differentiator coupled to the first electrode and configured to differentiate the output signal of the SiPM array, the differentiator configured to generate a differentiated signal from the output signal; anda pule counting circuit coupled to the differentiator and configured to generate pulses associated with the differentiated signal.44. The SiPM system according to claim 43 , wherein the pulse counting circuit includes a buffer and a comparator with a threshold.45. The SiPM system according to claim 44 , wherein the threshold is an adaptive threshold based on an envelope of the photon signal.46. The SiPM system according to claim 45 , wherein the pulse counting circuit delays the photon signal so as to arrive concurrently at the comparator with the adaptive threshold.47. The SiPM system according to ...

SYSTEMS AND METHODS FOR DETECTING PARTICLES IN A FLUID CHANNEL

Disclosed herein are systems and methods capable of identifying, tracking, and sorting particles or droplets flowing in a channel, for example, a microfluidic channel having a fluid medium. The channel and the fluid medium can have a similar refractive index such that they appear translucent or transparent when illuminated by electromagnetic radiation. The particles or droplets can have a refractive index substantially different from that of the channel and the medium, such that the particles or droplets interfere with the electromagnetic radiation. A sensor can be disposed adjacent to the channel to record the electromagnetic radiation. The sensor can be attached to a system for identifying, tracking, and sorting the droplets. 1. A system for detecting a particle in a channel , comprising:a channel comprising at least one particle dispersed in a medium, such that the at least one particle is moving from a first end of the channel to a second end of the channel;a source of electromagnetic radiation that illuminates at least a portion of the channel;a sensor to detect the at least one particle, wherein the sensor is positioned along a linear axis of the illuminated portion of the channel such that the sensor is substantially parallel to a direction of movement of the at least one particle through the channel; andan optical system that focuses and aligns the illuminated portion of the channel to the sensor.2. The system of claim 1 , wherein the sensor comprises a linear charge-coupled sensor claim 1 , a linear complementary metal-oxide-semiconductor sensor claim 1 , any suitable optical sensor claim 1 , or any combination thereof.3. The system of claim 1 , wherein the channel is positioned in or on a substrate.4. The system of claim 1 , wherein a source of electromagnetic radiation comprises an optical system.5. The system of claim 1 , comprising a plurality of particles and/or a plurality of channels and/or a plurality of linear sensors.6. The system of claim 1 , ...

Methods, device and apparatus for evaluating electrical current threat effects at joints

A method and device is disclosed for measuring one or more physical properties of, and/or induced by, out-gassing products released from and/or trapped within a joint in response to a lightning strike or other electrical current threat. A device for measuring one or more physical properties of, and/or induced by, gases, plasma and/or particles released from a joint in response to an electrical current threat. The joint includes a fastener passing through a structure so that an end of the fastener protrudes from the structure. The device includes a containment member having a base surrounding an opening into a cavity, the containment member being arranged to be mounted over the end of the fastener to enclose the end of the fastener within the cavity and to seal the opening; and one or more sensors arranged to measure physical properties of gases, plasma and/or particles contained by the cavity.

MULTI-SPECTRAL FILTER PROFILING AND QUALITY CONTROL FOR FLOW CYTOMETRY

Disclosed is a system and method for characterizing optical filters in a flow cytometer and optionally checking the operation of detectors in the flow cytometer. In some embodiments, the system may utilize an LED board having an opening through which the fluorescence and side scatter beams, rays, or images pass and light emitting diodes around the opening that emit light having different spectral profiles. The different spectral profiles allow the system to identify the optical filters that are placed in the flow cytometer, to verify detector operation, to assist in instrumentation troubleshooting, and to provide a quantitative reference for detector comparison. 1. A flow cytometry system comprising:at least one sample illumination source, wherein each sample illumination source is configured to deliver light to a corresponding sample location, thereby causing sample light to be emitted by or scattered off of particles in the corresponding sample location;focusing optics that are configured to direct the sample light from each sample location along one or more optical paths, wherein each optical path passes through a corresponding one or more optical filter elements and terminates at a corresponding detector that is configured to produce output data indicative of the measured intensity of light reaching that detector;a calibration light source that is configured to independently emit different spectral profiles of calibration light, each spectral profile of calibration light having one or more peaks at different wavelengths, such that the emitted calibration light is directed along at least a portion of each of the optical paths;one or more processors; and receive the output data from each of the detectors responsive to that detector receiving calibration light from the calibration light source, and', 'determine filtering characteristics for the optical filter elements along each optical path by analyzing the output data produced during the emission of at least two ...

Microparticle measurement device

In a microparticle measurement device, a sample is passed through each channel in a multi-flow channel, and a predetermined linear area is illuminated with light. Measurement light originating from a microparticle in the sample, such as scattered or fluorescent light, is shaped into a parallel beam by an objective lens and passes through a first and second transmission portions. The beams transmitted through these two portions are converged as first and second measurement beams onto the same straight line by a cylindrical lens. The intensity of the interference light formed by these beams is detected with a detector. Meanwhile, the light emitted from the light source and passing through the multi-flow channel without hitting the microparticle falls through the objective lens onto a non-reflection portion and does not travel toward the cylindrical lens. Accordingly, only the interference light formed by the measurement beams is allowed to fall onto the detector.

SYSTEM AND METHOD FOR THREE-DIMENSIONAL MICRO PARTICLE TRACKING

The present invention provides system and method for three-dimensionally tracking micro particle motion wherein a dark-field condenser is configured to receive light field emitted from a light source and project the light field on a fluid sample having at least one particle thereby generating a scattered light field associated with the at least one particle, an objective lens is configured to receive the scattered light field, an image capturing unit coupled to the objective lens receives the scattered light field thereby generating at least one image of the fluid sample, and a controller is configured to couple to the image capturing unit for analyzing interference ring pattern corresponding to a specific particle in the at least one image and determining a tracking information associated with the specific particle along three-dimensional direction according to the size and center of the interference ring pattern. 1. A particle tracking system , comprising:a light source, configured to generate a light field;a dark-field condenser, configured to receive the light field and project an off-axis light field on a fluid sample having at least one particle thereby generating a scattered light field associated with the at least one particle;an objective lens, configured to receive the scattered light field;an image capturing unit, configured to couple to the objective lens for receiving the scattered light field thereby generating at least one image corresponding to the scattered light field, wherein the scattered light field having an interference ring pattern corresponding to a specific particle having a distance far away from a reference plane that is a plane where the specific particle forms a non-interfered image on an image plane of the image capturing unit; anda controller, configured to couple to the image capturing unit for analyzing the interference ring pattern corresponding to the specific particle in the at least one image and determining a tracking ...

Lens-Free Imaging

Embodiments described herein relate to lens-free imaging. One example embodiment may include a lens-free imaging device for imaging a moving sample. The lens-free imaging device may include a radiation source configured to emit a set of at least two different wavelengths towards the moving sample. The lens-free imaging device is configured to image samples for which a spectral response does not substantially vary for a set of at least two different wavelengths. The lens-free imaging device may also include a line scanner configured to obtain a line scan per wavelength emitted by the radiation source and reflected by, scattered by, or transmitted through the moving sample. The line scanner is configured to regularly obtain a line scan per wavelength. Either the radiation source or the line scanner is configured to isolate data of the at least two different wavelengths. 1. A lens-free imaging device for imaging a moving sample , comprising:a radiation source configured to emit a set of at least two different wavelengths towards the moving sample, wherein the lens-free imaging device is configured to image samples for which a spectral response does not substantially vary for a set of at least two different wavelengths; anda line scanner configured to obtain a line scan per wavelength emitted by the radiation source and reflected by, scattered by, or transmitted through the moving sample,wherein the line scanner is configured to regularly obtain a line scan per wavelength, andwherein either the radiation source or the line scanner is configured to isolate data of the at least two different wavelengths.2. The lens-free imaging device according to claim 1 , further comprising:a processor programmed to stitch the obtained line scans per wavelength to obtain, for each wavelength, an image of the sample.3. The lens-free imaging device according to claim 2 , wherein the processor is further programmed to recombine a plurality of images of the sample to obtain a combined image ...

Panel comprising Borrelia MHC multimers

Disclosed herein is a panel comprising one or more MHC multimers; and a panel comprising one or more pools of MHC multimers, wherein each pool comprises one or more MHC multimers; wherein said MHC multimers comprise an antigenic peptide P derived from a antigenic polypeptide selected from the group consisting of OppA, DbpA, FlhF, FlaB and P37-42; as well as uses thereof in the detection of -specific T cells and the diagnosis, treatment and monitoring of disease in an individual.

Measurement method, measuring device, and measurement program

A measurement method, a measurement device, and a measurement program for acquiring information related to lipid particles contained in a measurement sample prepared without using a fluorescent dye are provided. The problem is resolved by the measurement method for measuring the number of particles in a measurement sample prepared without using a fluorescent dye, the method including obtaining information related to lipid particles contained in the measurement sample based on a plurality of characteristic values regarding light scattering in each particle obtained by a flow cytometer from the individual particles contained in the measurement sample.

LENS-FREE HOLOGRAPHIC OPTICAL SYSTEM FOR HIGH SENSITIVITY LABEL-FREE MICROBIAL GROWTH DETECTION AND QUANTIFICATION FOR SCREENING, IDENTIFICATION, AND SUSCEPTIBILITY TESTING

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

Multiple-Spot Time-Sequential Cytometry

An image flow cytometer for observing a microparticulate sample includes a flow chamber having a flow channel that permits the microparticulate sample to travel in a flow direction. An irradiation system scans an irradiation spot across a sensing area of the flow channel in a scan direction different from the flow direction. A detection system detects resultant light from the sensing area and provides a detection signal. An alignment system alters a location of the sensing area with respect to the flow chamber. A control unit causes the irradiation system to scan the irradiation spot during a first measurement interval and operates the alignment system to translate the location of the sensing area along the flow direction. The flow chamber can be mounted to a movable stage in some examples, and the alignment system can move the flow chamber substantially opposite the flow direction using the stage. 1. (canceled)2. (canceled)3. (canceled)4. (canceled)5. (canceled)6. (canceled)7. (canceled)8. A method of observing a microparticulate sample , the method comprising:passing the microparticulate sample through a flow chamber of an image flow cytometer along a flow direction;scanning an irradiation spot smaller than the microparticulate sample across a sensing area of the flow chamber, the scanning comprising translating the irradiation spot substantially along a scan direction different from the flow direction;detecting, contemporaneously with scanning, a time-varying intensity of resultant light from the flow chamber and providing a corresponding first intensity signal of the microparticulate sample;subsequently, translating a relative position of the sensing area with respect to the flow chamber along the flow direction; andsubsequently, repeating the scanning and detecting and providing a second intensity signal of the microparticulate sample.9. The method according to claim 8 , wherein:the passing comprises providing a flow of carrier fluid through the flow chamber, ...