Combined acoustic excitation and standoff chemical sensing for remote detection of buried explosive charges

SENSING A NARROW FREQUENCY BAND OF RADIATION AND EXAMINING OBJECTS OR ZONES

TEMPERATURE STANDARD

Improved non-linear optical microscope

COHERENTLY CONTROL NONLINEAR ONE RAMANSPEKTROSKOPIE

SYSTEM AND PROCEDURE FOR THE EPIDETEKTIONSKOHÄRENZ ANTI- STOKES RAMAN STREUMIKROSKOPIE ONES

Detection systems and methods using coherent anti-Stokes Raman spectroscopy

Systems and methods for remote and/or portable detection are provided. The system can include a source (305) of coherent laser pulses, components (315,320) for converting the coherent laser pulses into first beam pulses (391) at a first wavelength value, second beam pulses (392) at a second wavelength value, and third beam pulses (393) at a third wavelength value. Systems can further include optical components (330) configured to delay at least one of the first beam pulses, the second beam pulses, and the third beam pulses in order to create delayed beam pulses, and a focusing component (340) configured direct a substantially collinear combination of the delayed beam pulses and two of a set of: the first beam pulses, the second beam pulses, and the third beam pulses, onto a sample (355).

Methods of analyzing a reservoir fluid sample during or after collection of the sample using an analyzer

A method of analyzing a fluid sample from a reservoir comprises: collecting the sample in a sample container, wherein the sample container includes a sample receptacle, and wherein the step of collecting comprises allowing or causing the sample to flow into the sample receptacle; determining at least one property of the sample using an analyzer, wherein the analyzer is located at one end of the sample receptacle, wherein the step of determining comprises: (A) contacting the sample with radiated energy; and (B) detecting the interaction between the radiated energy and the sample, wherein the step of determining is performed during the step of collecting and during fluid flow of the sample into the sample receptacle. Another method comprises: transferring the sample from the sample container to a second container, wherein the step of determining is performed after the step of collecting and during fluid flow of the sample.

Method and device for the Raman spectroscopic, in ovo sex determination of fertilised and incubated birds' eggs

The invention relates to a method for the Raman spectroscopic, in ovo sex determination of fertilised and incubated birds' eggs (1), wherein the embryo, including the extra-embryonic structures, can move in the egg, and is not yet attached to the shell at the time of measuring. In addition, the following steps are carried out: monitoring the time course of the incubated egg until at least one recognisable blood vessel (21) is formed; creating a hole (2) in the shell in the region near to the attached blood vessel, using a hole-generating unit; finding the blood vessel forming in the egg using a vision system (19, 13) and a coaxial or lateral illumination with light (10a) in the visible wavelength range; positioning at least one blood vessel in the laser focus of a laser source (3), either by moving the egg or moving a lens (6) of a device (5) for introducing the laser light (3a), and detecting the Raman scattered radiation (7); registering the Raman scattered radiation of the irradiated ...

MANIPULATING THE TRANSLATION OF DNA STRANDS ACROSS AND THROUGH NANOPORE SEQUENCING SYSTEMS USING RAMAN SIGNATURES TO IDENTIFY DNA BASES AND METHODS

Nucleic acid sequencing methods and systems, the systems including nanochannel chip including: a nanochannel formed in an upper surface of the nanochannel chip and; a roof covering the nanochannel and comprising nanopores and a field enhancement structure; and a barrier disposed in the nanochannel. The method including: introducing a buffer solution including long-chain nucleic acids to the nanochannel chip; applying a voltage potential across the nanochannel chip to drive the nucleic acids through the nanochannel, towards the barrier, and to translocate the nucleic acids through nanopores adjacent to the barrier, such that bases of each of the nucleic acids pass through the field enhancement structure one base at a time and emerge onto an upper surface of the roof; detecting the Raman spectra of the bases of the nucleic acids as each base passes through the electromagnetic-field enhancement structure; and sequencing the nucleic acids based on the detected Raman spectra.

SINGLE NANOPARTICLE HAVING A NANOGAP BETWEEN A CORE MATERIAL AND A SHELL MATERIAL, AND MANUFACTURING METHOD THEREOF

The present invention relates to nanoparticles including a core and a shell surrounding the core between which a nanogap is formed, and a manufacturing method thereof, which can be usefully used for Raman analysis because they have a very high signal-amplifying effect and reproducibility by means of a plasmonic-coupling effect caused by the nanogap. Also, the present invention provides a method for detecting an analyte using the nanoparticles, and a kit for detecting an analyte containing the nanoparticles.

Method and Apparatus for Selective Suppression of Coherent Anti-Stokes Raman Scattering Signal

A METHOD AND DEVICE FOR DETECTING SMALL NUMBERS OF MOLECULES USING SURFACE-ENHANCED COHERENT ANTI-STOKES RAMAN SPECTROSCOPY

The device and method disclosed herein concern detecting, identifying, and or quantifying analytes, such as nucleic acids, with high resolution and fast response times using surface enhanced coherent anti-Stokes Raman spectroscopy. In certain embodiments of the invention, a small number molecular sample of the analyte (210) such as a nucleotide, passes through a microfluidic channel, microchannel, or nanochannel (185) and sample cell (175) that contains Raman-active surfaces, and is detected by surface enhanced, coherent anti-Stokes Raman spectroscopy (SECARS). Other embodiments of the invention concern an apparatus for analyte detection.

METHODS AND APPARATUSES FOR LABEL-FREE PARTICLE ANALYSIS

An apparatus to provide a label-free or native particle analysis comprises a light generating system producing first light pulses at a first wavelength and second light pulses at a second wavelength; and a flow cell coupled to the light generating system to convey particles for analysis. The light generating system is configured to chirp at least one of the first light pulses and the second light pulses to analyze the particles. 1. An apparatus , comprising:a light generating system producing a set of first light pulses at a first wavelength and a set of second light pulses at a second wavelength;a flow cell coupled to the light generating system to convey, in a flow, particles for analysis; anda first optical system coupled to the light generating system to chirp at least one of the first light pulses and the second light pulses to analyze the particles.2. The particle analysis apparatus of claim 1 , wherein the second wavelength is different from the first wavelength and wherein the set of first light pulses is only one light pulse at the first wavelength and the set of second light pulses is only one light pulse at the second wavelength.3. The particle analysis apparatus of claim 1 , further comprising:a collecting and analyzing system to collect and analyze light resulting from an interaction of the first light pulses and the second light pulses with the particles.4. The particle analysis apparatus of claim 1 , further comprising:a second optical system to combine the first light pulses and the second light pulses to deliver to the particles, wherein the particles are each exposed to a single combined light pulse from one of the first light pulses and one of the second light pulses and wherein the first light pulse is positively chirped and the second light pulse is negatively chirped.5. The particle analysis apparatus of claim 1 , further comprising:a synchronization system to synchronize the first light pulses and the second light pulses.6. The particle ...

Multiplex coherent raman spectroscopy detector and method

A multiplex coherent Raman spectrometer (10) and spectroscopy method rapidly detects and identifies individual components of a chemical mixture separated by a separation technique, such as gas chromatography. The spectrometer (10) and method accurately identify a variety of compounds because they produce the entire gas phase vibrational Raman spectrum of the unknown gas. This is accomplished by tilting a Raman cell (20) to produce a high-intensity, backward-stimulated, coherent Raman beam of 683 nm, which drives a degenerate optical parametric oscillator (28) to produce a broadband beam of 1100-1700 nm covering a range of more than 3000 wavenumber. This broadband beam is combined with a narrowband beam of 532 nm having a bandwidth of 0.003 wavenumbers and focused into a heated windowless cell (38) that receives gases separated by a gas chromatograph (40). The Raman radiation scattered from these gases is filtered and sent to a monochromator (50) with multichannel detection.

Method for forming a nanostructure penetrating a layer

A method for forming a nanostructure penetrating a layer and the device made thereof is disclosed. In one aspect, the device has a substrate, a layer present thereon, and a nanostructure penetrating the layer. The nanostructure defines a nanoscale passageway through which a molecule to be analyzed can pass through. The nanostructure has, in cross-sectional view, a substantially triangular shape. This shape is particularly achieved by growth of an epitaxial layer having crystal facets defining tilted sidewalls of the nanostructure. It is highly suitably for use for optical characterization of molecular structure, particularly with surface plasmon enhanced transmission spectroscopy.

Spectrally encoded coherent ant-stokes raman scattering endoscope

Disclosed is a spectrally encoded coherent anti-Stokes Raman scattering (CARS) endoscope that is capable of spatially encoding spectral dispersions of two light sources having frequency difference as much as a Raman shift and overlapping two laser beams on a position where a sample to be measured is placed, thereby acquiring a spatial distribution of CARS signals.

Erzeugung von zwei synchronisierten Laserimpulszügen

Verfahren zur Erzeugung von zwei synchronisierten Laserimpulszügen, das die folgenden Schritte aufweist:- Erzeugung eines Zugs von Seed-Laserimpulsen,- Teilung des Zugs von Seed-Laserimpulsen in einen ersten Impulszug und einen zweiten Impulszug;- Frequenzverschiebung des ersten Impulszugs durch Soliton-Eigenfrequenzverschiebung in einem optisch gepumpten Wellenleiter (14) mit anomaler Dispersion, wobei das Spektrum des frequenzverschobenen ersten Impulszugs durch Veränderung der Pumpleistung durchgestimmt wird, wobei es sich bei dem optisch gepumpten Wellenleiter (14) um eine seltenerddotierte Faser mit großer Modenfläche von mindestens 500 µm, vorzugsweise mindestens 1000 µmoder um eine seltenerddotierte Faser einer höheren Modenordnung, die eine Modenfläche von mindestens 2000 µm, vorzugsweise mindestens 5000 µmaufweist, oder um eine seltenerddotierte stabartige Faser, die eine Modenfläche von mindestens 2000 µm, vorzugsweise mindestens 5000 µmaufweist, oder um eine seltenerddotierte ...

Nucleic acid sequence identification

Spatially-offset optical coherence tomography

IMMUNOASSAY MEANS RAMANSPEKTROSKOPIE UNDER INTENSIFYING SURFACE CO-OPERATION (SERS)

PROCEDURE AND DEVICE FOR THE MONITORING OF HYDROGEN GAS AND A HYDROGEN FLAME

METALLIZED NANO-CRYSTALLINE SILICON AS AN ACTIVE SURFACE-EXTENDED SUBSTRATE FOR THE RAM ON SPECTROSCOPY (SERS)

Method and device for the Raman spectroscopic, in ovo sex determination of fertilised and incubated birds' eggs

The invention relates to a method for the Raman spectroscopic, in ovo sex determination of fertilised and incubated birds' eggs (1), wherein the embryo, including the extra-embryonic structures, can move in the egg, and is not yet attached to the shell at the time of measuring. In addition, the following steps are carried out: monitoring the time course of the incubated egg until at least one recognisable blood vessel (21) is formed; creating a hole (2) in the shell in the region near to the attached blood vessel, using a hole-generating unit; finding the blood vessel forming in the egg using a vision system (19, 13) and a coaxial or lateral illumination with light (10a) in the visible wavelength range; positioning at least one blood vessel in the laser focus of a laser source (3), either by moving the egg or moving a lens (6) of a device (5) for introducing the laser light (3a), and detecting the Raman scattered radiation (7); registering the Raman scattered radiation of the irradiated ...

Detection systems and methods using coherent anti-Stokes Raman spectroscopy

Systems and methods for remote and/or portable detection are provided. The system can include a source (305) of coherent laser pulses, components (315,320) for converting the coherent laser pulses into first beam pulses (391) at a first wavelength value, second beam pulses (392) at a second wavelength value, and third beam pulses (393) at a third wavelength value. Systems can further include optical components (330) configured to delay at least one of the first beam pulses, the second beam pulses, and the third beam pulses in order to create delayed beam pulses, and a focusing component (340) configured direct a substantially collinear combination of the delayed beam pulses and two of a set of: the first beam pulses, the second beam pulses, and the third beam pulses, onto a sample (355).

QUANTITATIVE OPTICAL MICROSCOPY USING A NON-LINEAR PATTERNED BEAM

PROCESS AND SYSTEM OF COHERENT NON-LINEAR MICROSCOPY HAVE MODULATION OF FOCAL VOLUME TO PROBE THE NANOSTRUCTURATION OF MATERIUAX ORGANIZE

L'invention concerne un procédé de caractérisation dimensionnelle d'un matériau structuré, dans lequel procédé : - on génère un faisceau laser d'excitation adapté à la microscopie non linéaire cohérente ; ce faisceau laser d'excitation étant focalisé dans un volume focal au sein du matériau structuré ; - on détecte des signaux émis par le matériau structuré ; - on élabore une pluralité de diagrammes d'émission correspondant chacune à une forme particulière du volume focal ; les formes particulières étant obtenues pour différents profils spatiaux non gaussien du front d'onde du faisceau laser d'excitation ; et - à partir des diagrammes d'émission ainsi élaborés, on en déduit des caractéristiques dimensionnelles dudit matériau structuré. The invention relates to a method for the dimensional characterization of a structured material, in which process: an excitation laser beam is generated which is suitable for coherent nonlinear microscopy; this excitation laser beam being focused in a focal volume within the structured material; signals emitted by the structured material are detected; a plurality of emission diagrams are produced, each corresponding to a particular form of the focal volume; the particular shapes being obtained for different non Gaussian spatial profiles of the wavefront of the excitation laser beam; and from the emission diagrams thus produced, dimensional characteristics of said structured material are deduced therefrom.

DEVICE FOR GENERATING A BEAM OF POLYCHROMATIC PHOTON ENERGY AND SUBSTANTIALLY CONSTANT

Un dispositif de génération (DG) comprend : • une source laser impulsionnelle (SL) délivrant des photons primaires présentant au moins une longueur d'onde dans des impulsions ayant une dissymétrie temporelle, • des moyens de mise en forme (MM) agissant sur les photons primaires pour délivrer un faisceau d'entrée (FE) focalisé et ayant une polarisation choisie, et • une fibre optique (FO) dans laquelle les photons primaires induisent des photons secondaires présentant différentes longueurs d'onde résultant d'une cascade de conversions Raman, et constituant un faisceau de sortie (FS) à spectre large et énergie sensiblement constante.

DEVICE FOR GENERATING A POLYCHROMATIC BEAM OF PHOTONS AND SPATIALLY AUTOADAPTE

METHODS FOR USING RAMAN SPECTROSCOPY TO OBTAIN A PROTEIN PROFILE OF A BIOLOGICAL SAMPLE

The invention provides methods for analyzing the protein content of a biological sample, for example to obtain a protein profile of a sample provided by a particular individual. The proteins and protein fragments in the sample are separated on the basis of chemical and/or physical properties and maintained in a separated state at discrete locations on a solid substrate or within a stream of flowing liquid. Raman spectra are then detected as produced by the separated proteins or fragments at the discrete locations such that a spectrum from a discrete location provides information about the structure or identity of one or more particular proteins or fragments at the discrete location. The proteins or fragments at discrete locations can be coated with a metal, such as gold or silver, and/or the separated proteins can be contacted with a chemical enhancer to provide SERS spectra. Method and kits for practicing the invention are also provided. © KIPO & WIPO 2007 ...

A METHOD FOR FORMING A NANOSTRUCTURE PENETRATING A LAYER

The device comprises a substrate, a layer present thereon and a nanostructure penetrating said layer, which nanostructure defines a nanoscale passageway through which a molecule to be analyzed can pass through the passageway, wherein the nanostructure has, in cross-sectional view, a substantially triangular shape. This shape is particularly achieved by growth of an epitaxial layer having crystal facets defining tilted sidewalls of the nanostructure. It is highly suitably for use for optical characterisation of molecular structure, particularly with surface plasmon enhanced transmission spectroscopy.

PULSE LASER LIGHT TIMING ADJUSTING DEVICE, ADJUSTING METHOD, AND OPTICAL MICROSCOPE

It is possible to provide a pulse laser beam timing adjusting device, a timing adjusting method, and an optical microscope capable of easily adjusting the pulse laser beam timing. The optical microscope includes: a pair of mirrors (21, 31) using the optical beam extracted from beam samplers (15, 16) to generate a first timing adjusting optical beam in which a first pulse laser beam (ω1) is delayed from a second pulse laser beam (ω2), and a second timing adjusting optical beam in which the second pulse laser beam (ω2) is delayed from the first pulse laser beam (ω1); a first detector (23) for outputting a first detection signal based on the nonlinear optical effect; a second detector (33) for outputting a second detection signal; and timing adjusting means (42) for adjusting the timing according to the first detection signal and the second detection signal.

SCANNER FOR SCANNING IMMEDIATE-SECTION SAMPLES

The present invention relates to a scanner for scanning immediate-section samples during surgery using an apparatus for carrying out and evaluating an imaging method using CARS effects or CARS-like effects.

DEVICE AND METHOD FOR EXAMINING SAMPLES IN A LIQUID

The invention relates to a device for examining samples (1) in a liquid (5), comprising a movable shaft (2) on which the sample (1) is secured and comprising a cuvette (4). The device further comprises a basin (3) which surrounds the movable shaft (3), and the basin (3) can be filled with the liquid (5). The movable shaft (2) is designed to receive the sample (1) on the shaft upper face (24), and the movable shaft (2) engages under the cuvette (4). The cuvette is open at least at the bottom and is designed such that the lower face of the cuvette is immersed into the liquid (5) in the basin (3). Furthermore, means are provided in order to generate a pressure difference between the interior of the cuvette (4) and the region outside the cuvette (4) such that the fill level (21) of the liquid (5) in the cuvette (4) can be adjusted. The invention further relates to a method for examining samples (1) in a liquid (5).

TUNABLE OPTICAL PARAMETRIC OSCILLATOR FOR NON-LINEAR VIBRATIONAL SPECTROSCOPY

A system is disclosed for providing a first electromagnetic field and a second electromagnetic field to vibrational analysis equipment that is responsive to a difference between first and second frequencies of the first and second electromagnetic fields respectively. The system includes a non-linear crystal that may be pumped at a high repetition rate to provide a pulsed signal field at a signal field frequency and a pulsed idler field at an idler field frequency as output. The signal field provides the first electromagnetic field and the idler field provides the second electromagnetic field. The system also includes a tuning system for permitting the difference between the signal field frequency and the idler field frequency to be changed. The system also includes an output unit for providing the first and second electromagnetic fields to the vibrational analysis equipment.

Device for generating a polychromatic and spatially self-adapted beam of photons

Some embodiments are directed to a generating device, including a pulse laser source providing primary photons having at least one wavelength, shaping device(s) acting on the primary photons to provide an input beam, a nonlinear crystal, and controller(s) generating, in the nonlinear crystal, at least one electric field that is synchronous with the input beam and suitable for inducing a phase mismatching in the nonlinear crystal through an electro-optical effect, in order to convert the primary photons of the input beam into secondary photons having wavelengths belonging to a supercontinuum.

Raman spectroscopic method for determining the ligand binding capacity of biologicals

A nondestructive process for determining the reactive capacity of a test biological by Raman scattering. The test biological may be any one of enzymes, enzyme cofactors, coenzymes, antibodies, antibody fragments, hemeproteins, peptides, synthetic peptides, toxins, toxoids, glycosphingolipids, lectins, lipids, lipid complexes, phospholipids, carbohydrates, saccharides, gangliosides, nucleic acids, fragments of nucleic acids, pathogen adhesion factors, receptors, receptor subunits, membranes, organelles, cells, tissues and complexes containing membranes, organelles, cells and tissues, or a bioconcentrator. The test biological is irradiated with a light source to produce a Raman scattering spectrum of the irradiated biological. The Raman scattering spectrum is collected and processed to determine the ability of the test biological to react with ligands. The analyzing step includes comparing the Raman scattering spectrum of the test biological against that of a biological standard of the same ...

Pulse Laser Light Timing Adjusting Device, Adjusting Method, and Optical Microscope

To provide a pulse laser light timing adjusting device capable of easily adjusting pulse laser light timing, a timing adjusting method, and an optical microscope. An optical microscope in accordance with the present invention includes mirror pairs 21 and 31 to generates the first timing adjusting optical beam in which the first pulse laser light 1 is delayed from the second pulse laser light 2 and the second timing adjusting optical beam in which the second pulse laser light 2 is delayed from the first pulse laser light having a first wavelength from the optical beam extracted at the beam samplers 15 and 16, a first detector 23 and a second detector 33 to output a first detection signal and a second detection signal based on a nonlinear optical effect, and timing adjusting means 42 to adjust the timing based on the first detection signal and the second detection signal.

COHERENTLY CONTROLLED NONLINEAR RAMAN SPECTROSCOPY AND MICROSCOPY

A method and system are presented for producing an output coherent anti-stokes Raman scattering (CARS) signal of a medium. The method comprises generation of a unitary optical excitation pulse that carries a pump photon, a Stokes photon and a probe photon; and inducing a CARS process in the medium by exciting the medium by the at least one such unitary optical excitation pulse.

Pathogen Detection Using Coherent Anti-Stokes Raman Scattering Microscopy

The invention provides a system and method for automatic real-time monitoring for the presence of a pathogen in water using coherent anti-stokes Raman scattering (CARS) microscopy. Water sample trapped in a trapping medium is provided to a CARS imager. CARS images are provided to a processor for automatic analyzing for the presence of image artifacts having pre-determined features characteristic to the pathogen. If a match is found, a CARS spectrum is taken and compared to a stored library of reference pathogen-specific spectra for pathogen identification. The system enables automatic pathogen detection in flowing water in real time.

Device and method for examining samples in a liquid

Moreover, the invention relates to a method for examining samples (1) in a liquid (5).

NEXT GENERATION FLOW CYTOMETER SORTER

COHERENT NONLINEAR MICROSCOPY SYSTEM AND METHOD WITH VARIATION OF THE FOCAL VOLUME IN ORDER TO PROBE THE NANOSTRUCTURE OF ORGANIZED MATERIALS

APPARATUS REGULATING NUCLEOTIDE RELEASE DURING A REACTION

DETEKTION VON STRAHLUNG IN EINEM SCHMALEN FREQUENZBAND UND PRUEFUNG VON OBJEKTEN ODER GEBIETEN.

A method and device for detecting small numbers of molecules using surface-enhanced coherent anti-stokes raman spectroscopy

The device and method disclosed herein concern detecting, identifying, and or quantifying analytes, such as nucleic acids, with high resolution and fast response times using surface enhanced coherent anti-Stokes Raman spectroscopy. In certain embodiments of the invention, a small number molecular sample of the analyte (210) such as a nucleotide, passes through a microfluidic channel, microchannel, or nanochannel (185) and sample cell (175) that contains Raman-active surfaces, and is detected by surface enhanced, coherent anti-Stokes Raman spectroscopy (SECARS). Other embodiments of the invention concern an apparatus for analyte detection.

External modification of composite organic inorganic nanoclusters

Modified and functionalized metallic nanoclusters capable of providing an enhanced Raman signal from an organic Raman-active molecule incorporated therein are provided. For example, modifications include coatings and layers, such as adsorption layers, metal coatings, silica coatings, and organic layers. The nanoclusters are generally referred to as COINs (composite organic inorganic nanoparticles) and are capable of acting as sensitive reporters for analyte detection. A metal that enhances the Raman signal from the organic Raman-active compound is inherent in the nanocluster. A variety of organic Raman-active compounds and mixtures of compounds can be incorporated into the nanocluster.

Optical sources

THAT TURNS OUT THE RELEASE OF NUCLEOTIDES DURING A REACTION STEERS

MICROSCOPIC DEVICE AND PROCEDURE OF MEANS OF POLARIZING COHERENT ANTI- STOKESRAMANSTREUUNG

SYSTEM AND PROCEDURE FOR HOCHSENSITIVE VIBRATION PICTURE GIVING WITH FREQUENCY-MODULATED COHERENT ANTI-STOKES RAM TO FAITHFUL ANALYSIS

Method for analyzing biological specimens by spectral imaging

A method for analyzing biological specimens by spectral imaging to provide a medical diagnosis includes obtaining spectral and visual images of biological specimens and registering the images to detect cell abnormalities, pre-cancerous cells, and cancerous cells. This method eliminates the bias and unreliability of diagnoses that is inherent in standard histopathological and other spectral methods. In addition, a method for correcting confounding spectral contributions that are frequently observed in microscopically acquired infrared spectra of cells and tissue includes performing a phase correction on the spectral data. This phase correction method may be used to correct various types of absorption spectra that are contaminated by reflective components.

Methods of analyzing a reservoir fluid sample during or after collection of the sample using an analyzer

A method of analyzing a fluid sample from a reservoir comprises: collecting the sample in a sample container, wherein the sample container includes a sample receptacle, and wherein the step of collecting comprises allowing or causing the sample to flow into the sample receptacle; determining at least one property of the sample using an analyzer, wherein the analyzer is located at one end of the sample receptacle, wherein the step of determining comprises: (A) contacting the sample with radiated energy; and (B) detecting the interaction between the radiated energy and the sample, wherein the step of determining is performed during the step of collecting and during fluid flow of the sample into the sample receptacle. Another method comprises: transferring the sample from the sample container to a second container, wherein the step of determining is performed after the step of collecting and during fluid flow of the sample.

SENSING A NARROW FREQUENCY BAND OF RADIATION AND GEMSTONES

DIAMOND DETECTION USING COHERENT ANTI-STOKES RAMAN SPECTROSCOPY

The invention concerns a method and apparatus for detecting diamonds using the phenomenon of coherent anti-Stokes Raman spectroscopy (CARS). Each particle (46) which is to be analysed is irradiated by a focused particle irradiating beam (44) of laser light formed by combining initial laser beams (35, 36) having frequencies differing from one another by a value characteristic for diamond. Components of the initial laser beams are coherently phase-matched to produce, in diamond particles, a CARS signal characteristic of diamond. The scattered signal from the particle is then collected and analysed for the presence of the characteristic CARS signal. The irradiating beam and/or at least one of the initial laser beams is focused by a lens system including a cylindrical lens. This feature has been found to give an enhanced result compared to conventional CARS analysis.

OPTICAL FIBER FOR COHERENT ANTI-STOKES RAMAN SCATTERING ENDOSCOPES

An optical fiber for use in a Coherent Anti-Stokes Raman Scattering (CARS) endoscope, comprising a core guiding lightwaves at a pump wavelength and at a Stokes wavelength, the core being single-mode at both wavelengths. The core is surrounded by cladding layers, including an inner cladding layer, a trench cladding layer, an intermediate cladding layer and an outer cladding layer. The refractive index of the trench cladding layer is lower than those of both neighboring cladding layers so as to define a trench in the radial refractive-index profile. The bending losses of the fundamental LP01 mode of the fiber at the Stokes wavelength are limited while maintaining high confinement losses for the higher-order LP11 mode of the fiber at the pump wavelength. The combination of the intermediate and outer cladding layers forms a multimode waveguide for guiding a collected CARS signal generated by an object or medium probed with the endoscope.

METHOD FOR ANALYZING BIOLOGICAL SPECIMENS BY SPECTRAL IMAGING

A method for registering a visual image and a spectral image of a biological sample includes aligning a first set of coordinate positions of a plurality of reticles on a slide holder and a second set of coordinate positions of the plurality of reticles on the slide holder. The method further includes generating a registered image of a visual image of a biological sample and a spectral image of the biological sample based upon the alignment of the first and second set of coordinate positions.

METHOD FOR ANALYZING BIOLOGICAL SPECIMENS BY SPECTRAL IMAGING

A method for analyzing biological specimens by spectral imaging to provide a medical diagnosis includes obtaining spectral and visual images of biological specimens and registering the images to detect cell abnormalities, pre-cancerous cells, and cancerous cells. This method eliminates the bias and unreliability of diagnoses that is inherent in standard histopathological and other spectral methods. In addition, a method for correcting confounding spectral contributions that are frequently observed in microscopically acquired infrared spectra of cells and tissue includes performing a phase correction on the spectral data. This phase correction method may be used to correct various types of absorption spectra that are contaminated by reflective components.

DEVICE, APPARATUS AND METHOD FOR PROVIDING PHOTOSTIMULATION AND IMAGING OF STRUCTURES

According to exemplary embodiments of the present disclosure, it is possible to provide method, system, arrangement, computer-accessible medium and device to stimulate individual neurons in brain slices in any arbitrary spatio-temporal pattern, using two-photon uncaging of photo-sensitive compounds such as MNI- glutamate and/or RuBi-Glutamate with beam multiplexing. Such exemplary method and device can have single-cell and three-dimensional precision. For example, by sequentially stimulating up to a thousand potential presynaptic neurons, it is possible to generate detailed functional maps of inputs to a cell. In addition, it is possible to combine this exemplary approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity. Further exemplary embodiments of the present disclosure can include a light-weight, compact portable device providing for uses in a wide variety of applications.

COMPACT LASER DIODE MONITOR AND METHOD

A first compact laser diode monitor (30) for measuring concentrations of individual atomic and molecular species in both gaseous and semitransparent fluids, includes a first diode laser (36) having an output for resonantly exciting a first selected electronic transition of a specific atomic or molecular species of a sample (34), and a second diode laser (38) having an output for resonantly exciting a second selected electronic transition of said specific atomic or molecular species of said sample (34). A difference between said first and second frequency outputs is equal to a vibrational energy level difference of the specific atomic or molecular species. Further said first and second frequency outputs are simultaneously directed into said sample (34), with selected momentum vectors in order to cause the selected atomic or molecular species to emit a coherent photon signal in a selected direction having a frequency equal to twice the first frequency output minus the second frequency output ...

Single nanoparticle having a nanogap between a core material and a shell material, and manufacturing method thereof

METHOD FOR DETECTING AN OPTICAL SIGNAL NOT RESONANT LINEAR AND DEVICE FOR IMPLEMENTING SAID METHOD

DEVICE FOR GENERATING A POLYCHROMATIC BEAM OF PHOTONS AND SPATIALLY AUTOADAPTE

Un dispositif de génération (DG) comprend : • une source laser impulsionnelle (SL) délivrant des photons primaires présentant au moins une longueur d'onde, • des moyens de mise en forme (MM) agissant sur les photons primaires pour délivrer un faisceau d'entrée, • un cristal non linéaire (CN), et • des moyens de contrôle (MC) générant dans le cristal non linéaire (CN) au moins un champ électrique synchrone avec le faisceau d'entrée et propre à induire dans ce dernier (CN) un désaccord de phase par un effet électro-optique pour convertir les photons primaires du faisceau d'entrée en des photons secondaires ayant des longueurs d'onde appartenant à un super-continuum.

CASCADE TYPE INTERFEROMETRIC NONLINEAR IMAGING APPARATUS BASED ON COHERENT ANTI-STOKES RAMAN SCATTERING CAPABLE OF IMPROVING MEDIUM MOLECULE DETECTING SENSITIVITY

PURPOSE: A cascade type interferometric nonlinear imaging apparatus based on CARS(Coherent Anti-Stokes Raman Scattering) is provided to improve medium molecule detecting sensitivity of a measuring sample by exciting medium molecules of a standard sample to allow the phase difference between beams passing through the measuring sample to be modulated. CONSTITUTION: A cascade type interferometric nonlinear imaging apparatus based on CARS comprises a light source(10), a phase change device(30), a scan device(40) and a detector(60). The light source generates stokes light(1) having arbitrary frequency band and pump light(2) for exciting medium molecules of a sample with the stokes light. The phase change device allows the Stokes light and the pump light generated from the light source to pass through a standard sample(20), and changes the phase of the passed light and anti-stokes light on the same path. The scan device scans the phase-changed light to the space of a measuring sample(50). The ...

CARS 현미경 및 이의 제어 방법

CARS 현미경이 개시된다. 본 CARS 현미경은 복수의 빔을 발생시키는 광원, 발생된 복수의 빔을 이용하여 중첩된 빔을 생성하는 이색 거울, 상기 중첩된 빔을 시료 내부로 집속하는 대물렌즈, 시료에서 발생된 CARS 신호의 사이드 로브(side lobes)를 제거하는 공초점 핀홀(confocal pinhole), 및 사이드 로브가 제거된 CARS 신호를 검출하는 광검출기를 포함한다.

Topographic phase control for overlay measurement

Metrology tools and methods are provided, which estimate the effect of topographic phases corresponding to different diffraction orders, which result from light scattering on periodic targets, and adjust the measurement conditions to improve measurement accuracy. In imaging, overlay error magnification may be reduced by choosing appropriate measurement conditions based on analysis of contrast function behavior, changing illumination conditions (reducing spectrum width and illumination NA), using polarizing targets and/or optical systems, using multiple defocusing positions etc. On-the-fly calibration of measurement results may be carried out in imaging or scatterometry using additional measurements or additional target cells.

Modification of metal nanoparticles for improved analyte detection by surface enhanced raman spectroscopy (SERS)

A SERS active particle having a metal-containing particle and a cationic coating on the metal-containing particle, wherein the SERS active particle carries a positive charge is disclosed. Also, a SERS active particle having a metal-containing particle and a non-metallic molecule, wherein the metal-containing particle is derivatized with the non-metallic molecule is disclosed. In addition, several methods of modifying the nanoparticles surfaces of a SERS active particle and of improving the interaction between the SERS active particle and an analyte are disclosed.

SYSTEM AND METHOD FOR HIGH SENSITIVITY VIBRATIONAL IMAGING WITH FREQUENCY MODULATION COHERENT ANTI-STOKES RAMAN SCATTERING ANALYSES

A system is disclosed for detecting a nonlinear coherent field induced in a sample. The system includes optics, a modulation system, and a detector system. The optics are for directing a first electromagnetic field at a first frequency Ωi and a second electromagnetic field at a second frequency Ω2 toward a focal volume such that a difference frequency Ω1-Ω2 is resonant with a vibrational frequency of a sample in the focal volume. The modulation system is for modulating the difference frequency Ωi-Ω2 such that the difference frequency ΩrΩ2 is tuned in and out of the vibrational frequency of the sample at a modulation frequency. The detector system is for detecting an optical field that is generated through non-linear interaction of Ω1 and Ω2 and the sample responsive to the modulation frequency. The system is in particular intended for use in coherent Anti-Stokes Raman Spectroscopy (CARS).

METHODS FOR USING RAMAN SPECTROSCOPY TO OBTAIN A PROTEIN PROFILE OF A BIOLOGICAL SAMPLE

The invention provides methods for analyzing the protein content of a biological sample, for example to obtain a protein profile of a sample provided by a particular individual. The proteins and protein fragments in the sample are separated on the basis of chemical and/or physical properties and maintained in a separated state at discrete locations on a solid substrate or within a stream of flowing liquid. Raman spectra are then detected as produced by the separated proteins or fragments at the discrete locations such that a spectrum from a discrete location provides information about the structure or identity of one or more particular proteins or fragments at the discrete location. The proteins or fragments at discrete locations can be coated with a metal, such as gold or silver, and/or the separated proteins can be contacted with a chemical enhancer to provide SERS spectra. Method and kits for practicing the invention are also provided.

METHOD AND OPTICAL ARRANGEMENT FOR GENERATING A NONLINEAR OPTICAL SIGNAL ON A MATERIAL WHICH IS EXCITED BY AN EXCITATION FIELD, AND USE OF THE METHOD AND OF THE OPTICAL ARRANGEMENT

The invention relates to a method and an optical arrangement (10) for generating a nonlinear optical signal (17) on a material (16) which is excited by an excitation field (2). With the excitation field, coherent fields of first and second optical pulses of different frequencies are overlapped in the material (16) both temporally and spatially. The first pulse of a first frequency is generated in a first beam (3) of a first optical generator unit (1), and the second pulse of a second frequency is generated in a second beam (8) of a second optical generator unit which is synchronously pumped by the first optical generator unit (1). With the first pulses of the first frequency as the fundamental frequency pulse (5) of a higher harmonic frequency, the same is generated, and the second optical generator unit (7) is pumped with the pulses of the higher harmonic frequency. According to the concept of the invention, the method proposes that the second optical generator unit (7), as an optical ...

AUTOFOCUS MECHANISM FOR SPECTROSCOPIC SYSTEM

The present invention provides an autofocus mechanism for a spectroscopic system (400) that is adapted to determine a property of a volume of interest. The volume of interest has an optical property that varies with time. The invention provides measurement means that are adapted to measure the fluctuations of the optical property of the volume of interest for determining the position (428) of the volume of interest. The spectroscopic system being further adapted to focus an excitation beam (418) into the determined volume of interest and for collecting return radiation (420) emanating from the volume of interest for spectroscopic analysis. Preferably, inelastically scattered radiation of an excitation beam (428) is separated from elastically scattered radiation for spectroscopic analysis. The elastically scattered radiation of the excitation beam is in turn exploited in order to measure the fluctuations of the optical property of the volume of interest. Making use of a control loop allows for maximization of the amplitude and/or intensity of the fluctuation inherently specifying the position of a volume of interest e.g. the center of a capillary vessel (450).

Composite organic inorganic nanoclusters

Metallic nanoclusters capable of providing an enhanced Raman signal from an organic Raman-active molecule incorporated therein are provided. The nanoclusters may be further functionalized, for example, with coatings and layers, such as adsorption layers, metal coatings, silica coatings, probes, and organic layers. The nanoclusters are generally referred to as COINs (composite organic inorganic nanoparticles) and are capable of acting as sensitive reporters for analyte detection. A variety of organic Raman-active compounds and mixtures of compounds can be incorporated into the nanocluster.

Surface-enhanced Raman spectroscopy immunoassay

A method, composition, device, apparatus, and kit for the determination of the presence or amount of an analyte by monitoring an analyte-mediated ligand binding event in a test mixture which contains the analyte to be assayed, a specific binding member, a Raman-active label, and a particulate having a surface capable of inducing a surface-enhanced Raman light scattering. The test mixture is illuminated with a radiation sufficient to cause the Raman-active label in the test mixture to emit a detectable Raman spectrum. The differences in the detected surface-enhanced Raman scattering spectra are dependent upon the amount of the analyte present in the test mixture. Thus, by monitoring these differences, the presence or amount of the analyte are determined.

Accessories for Raman and Luminescence Spectral Acquisitions

The present invention provides for a novel series of accessories for Raman and/or luminescence spectral acquisitions for many different applications and methods for making such accessories. The invention further provides sample holders that enhance sample handling ability and sample sensitivity, reduce fluorescence and Raman background, as well as sample size and consumption, and thereby improve resulting spectral analyses.

Spectroscopic methods for body fluid age determination

The present invention relates to a method of determining the age of a body fluid stain in a sample. This method involves providing the sample containing a body fluid stain; providing a statistical model for determination of the age of the body fluid stain in the sample; subjecting the sample or an area of the sample containing the stain to a spectroscopic analysis to produce a spectroscopic signature for the sample; and applying the spectroscopic signature for the sample to the statistical model to ascertain the age of the body fluid stain in the sample. A method of establishing a statistical model for determination of the age of a body fluid stain in a sample is also disclosed.

Stimulated and coherent anti-stokes raman spectroscopic methods for the detection of molecules

Spectroscopic analysis systems and methods for analyzing samples are disclosed which exploit inelastically scattering radiation to amplify optical signals from an irradiated sample. Samples are irradiated in a chamber having a resonant cavity containing a plurality of affixed reflectors, where selective Stokes scattered radiation is transmitted to a detector for determination of sample identity. Coupling the spectroscopic analysis system and method with a nucleic acid sequencing system is also disclosed for determining nucleic acid sequences.

System and method for pathogen detection and identification

This disclosure concerns a pathogen inspection system that includes a handling system that presents single cells from a sample to at least one QCL laser source. The QCL laser source is configured to deliver light to a cell within the cells in order to induce vibrational bond absorption in one or more analytes within the cell. The system includes a detection facility that detects the mid-infrared wavelength light transmitted by the cell and identifies the cell as either a pathogen or not a pathogen.

Methods and apparatuses for label-free particle analysis

An apparatus to provide a label-free or native particle analysis comprises a light generating system producing first light pulses at a first wavelength and second light pulses at a second wavelength; and a flow cell coupled to the light generating system to convey particles for analysis. The light generating system is configured to chirp at least one of the first light pulses and the second light pulses to analyze the particles.

SYSTEM AND METHOD FOR HIGH SENSITIVITY VIBRATIONAL IMAGING WITH FREQUENCY MODULATION COHERENT ANTI-STOKES RAMAN SCATTERING ANALYSES

SINGLE NANOPARTICLE HAVING A NANOGAP BETWEEN A CORE MATERIAL AND A SHELL MATERIAL, AND PREPARATION METHOD THEREOF

Method for producing excitation radiation for multi species analyzing of e.g. drugs in forensic medicine, involves combining and emitting converted radiations of partial radiation beams as excitation radiations to sample

The method involves producing pump pulses for exciting molecules of a sample from a base condition to a virtual level and sample pulses for pumping the molecules to high virtual level by a laser source (10) i.e. microchip laser. Narrow band radiations of the source are divided into two partial radiation beams (13, 14), and the radiations of one of the beams are converted into wide band signal pulses. The radiations of the other beam are converted into narrow band pump and/or sample pulses, and the converted radiations are combined and emitted as excitation radiations to the sample. Independent claims are also included for the following: (1) a device for producing excitation radiation comprising a device for converting laser radiations into excitation radiations (2) a device for analyzing a sample comprising a spectrometer for detecting and representing scattered signals.

Combined acoustic excitation and standoff chemical sensing for the remote detection of buried explosive charges

Dual mode coherent anti-Stokes Raman (CARS)/absorption spectroscopy sensor

A means for sensing a target gas comprises: a single source 115 (e.g. one or more lasers, laser and non-liner optical element) for generating two light beams having respective first and second frequencies, wherein the difference between the two frequencies is the Raman frequency of the target gas; a difference frequency generator 104 which outputs the two light beams along with a third light beam having a third frequency equal to the difference between the first and second frequencies towards the target gas; an input optic 120 and splitter 140 for directing light from the third light beam, after interacting with the target gas, to a receiver 150 for absorption spectroscopy processing, and for directing a fourth light beam from the target gas to a receiver 160 for coherent anti-Stokes Raman processing, wherein the fourth light beam has a frequency of twice the first frequency minus the second frequency.

TI-DOPED SAPPHIRE LASER-OPERATED DETECTOR

In a laser-operated detecting device, the light source of a laser beam applied to a specimen under test is a tunable laser whose wavelength can be continuously tuned in a certain range. The detecting device is realized as a high speed liquid chromatography fluorescence detector, a high speed liquid chromatography Raman detector, a laser Raman spectroscopic measuring device, a coherent anti-Stokes Raman measuring device, or a microscopic Raman measuring device.

A method and device for detecting small numbers of molecules using surface-enhanced coherent anti-stokes raman spectroscopy

NUCLEIC ACID SEQUENCE IDENTIFICATION

COHERENT EINIMPULS ANTI- STOKES RAMANSTREUMIKROSKOPIE ONES AND - SPECTROSCOPY

AUTOCFOCUS MECHANISM FOR A SPECTROSCOPIC SYSTEM

PROCEDURE AND OPTICAL ARRANGEMENT FOR PRODUCING A NONLINEAR OPTICAL SIGNAL AT A BY A SUGGESTION FIELD LIVELY MATERIAL AS WELL AS USE OF THE PROCEDURE AND THE OPTICAL ARRANGEMENT

Diamond detection using coherent anti-stokes raman spectroscopy

METHOD AND DEVICE FOR MONITORING HYDROGEN GAS AND HYDROGEN FLAME

A method for monitoring hydrogen gas and a hydrogen flame characterized in that the object light having a wavelength of about 309 nm resulting from two laser beams entering a space to be monitored is focused to form an electronic image, and the electronic image is amplified and converted back into an optical image, thereby creating an image showing a spatial intensity of a specific wavelength. A device for monitoring hydrogen gas and a hydrogen flame characterized by comprising two or more laser light source, focusing means for focusing the object light having a wavelength of about 309 nm resulting from laser beams in a space to be monitored, image-forming means for converting the object light into an electronic image, amplifying the electronic image, and converting it back into an optical image, and an imaging means for creating an image of spatial intensity distribution of a specific wavelength.

METHOD AND DEVICE FOR THE RAMAN SPECTROSCOPIC, IN OVO SEX DETERMINATION OF FERTILISED AND INCUBATED BIRDS' EGGS

The invention relates to a method for the Raman spectroscopic, in ovo sex determination of fertilised and incubated birds' eggs (1), wherein the embryo, including the extra-embryonic structures, can move in the egg, and is not yet attached to the shell at the time of measuring. In addition, the following steps are carried out: monitoring the time course of the incubated egg until at least one recognisable blood vessel (21) is formed; creating a hole (2) in the shell in the region near to the attached blood vessel, using a hole-generating unit; finding the blood vessel forming in the egg using a vision system (19, 13) and a coaxial or lateral illumination with light (10a) in the visible wavelength range; positioning at least one blood vessel in the laser focus of a laser source (3), either by moving the egg or moving a lens (6) of a device (5) for introducing the laser light (3a), and detecting the Raman scattered radiation (7); registering the Raman scattered radiation of the irradiated ...

RAMAN OPTRODE PROCESSES AND DEVICES FOR DETECTION OF CHEMICALS AND MICROORGANISMS

A methodology and devices for detecting or monitoring or identifying chemical or microbial analytes are described. The methodology comprises four basic steps: (1) The gas or liquid medium to be monitored or analyzed is brought into contact with a bioconcentrator which is used to bind with or collect and concentrate one or more analytes; (2) the bioconcentrator-analyte complex is then exposed to radiation of one or more predetermined wavelengths to produce Raman scattering spectral bands; (3) at least a portion of the Raman spectral bands are collected and processed by a Raman spectrometer to convert the same into an electrical signal; and (4) the electrical signal is processed to detect and identity, qualitatively and/or quantitatively, the analyte(s). The methodology of this invention may also comprise Raman reactive capacity analysis of the bioconcentrator itself, simultaneously with or independently from the detection of the analyte, to determine the potential ability of the bioconcentrator to complex with analytes; the results of this latter analysis may be used to affect or alter or modify the methodology involved in detection and analysis of the analytes. Also the invention is accomplished by a Raman Optrode comprising: a bioconcentrator capable of binding with the analyte(s); a mechanism or procedure or device for bringing the gas or liquid sample into contact with the bioconcentrator; a light source suitable for generating Raman scattering; a Raman spectrometer capable of collecting and processing the Raman scattering spectral information and translating it into an electrical signal; and electronic hardware and software for analyzing the electrical signal and translating the signal into information on the presence, identity and/or quantity of the bound analytes. Various forms of bioconcentrators are described, as well as a variety of analytes which may be detected, monitored, or identified by this invention, and a variety of devices which can be fabricated ...

FOURIER DOMAIN MODE LOCKING

A control system for improving and stabilizing Fourier domain mode locking (FDML) operation. The control system may also provide regulation of FDML operational parameters such as filter tuning, laser gain, polarization, polarization chromaticity, elliptical polarization retardance, and/or dispersion. The control system may be located internal to or external from the FDML laser cavity.

CARS microscope

QUANTITATIVE NON-LINEAR OPTICAL MICROSCOPY USING A BEAM FORMATTED

L'invention concerne un microscope optique non-linéaire utilisant un faisceau laser de Bessel de façon à imager un échantillon avec une bonne résolution latérale et une profondeur de champ étendue. Ce microscope intègre un scanner de lames. L'échantillon est disposé sur une lame, qui est chargée automatiquement sur une platine motorisée, de sorte que le faisceau laser peut balayer la totalité de la lame. Les signaux utiles provenant de l'échantillon sont combinés avec l'image conventionnelle du scanner de lames de façon à obtenir une image globale mettant clairement en évidence avec une bonne résolution des éléments d'intérêts.

METHOD AND DEVICE FOR MONITORING HYDROGEN GAS AND HYDROGEN FLAME

NONLINEAR SPECTROSCOPIC METHODS FOR IDENTIFYING AND CHARACTERIZING MOLECULAR INTERACTIONS

This invention provides methods and devices for identifying and/or characterizing interactions involving molecules, including, but not limited to, identifying and/or characterizing interactions involving target molecules and candidate molecules. The present invention provides methods using multidimensional infrared spectrographic techniques, such as four wave mixing and pump - probe techniques, for identifying interactions involving biomolecules and therapeutic candidate molecules, and for characterizing such interactions in terms of their binding coefficients and/or equilibrium constants.

LASER MICROSCOPE WITH A PHYSICALLY SEPARATING BEAM SPLITTER

The invention proposes a method for imaging at least one microscopic property of a sample (112) and an apparatus (110) with which the proposed method can be carried out, for example. In the method, at least one coherent illumination light (124) with at least one illumination wavelength is produced by means of at least one light source (114, 116). The illumination light (124) is imaged onto at least one region on or within the sample (112). Detection light (144) emitted by the sample (112) is split at least partially into incoherent detection light (148) and into coherent detection light (146) by means of at least one physically separating beam splitter (140). The coherent detection light (146) is at least partially separated from the coherent illumination light (124) by at least one additional beam-splitter element (160). The coherent detection light (146) is detected. The proposed method can be used in particular for investigating the sample (112) by means of coherent anti-Stokes-Raman scattering (CARS).

Laser microscope

Provided is a laser microscope, in which laser irradiation optical systems ( 3, 4, 5, 6 ) are employed for coaxially irradiating a sample with a CARS laser beam and a Raman scattering laser beam, and CARS light is detected by CARS light detecting means ( 12 ) and Raman scattering light is detected by Raman scattering light detecting means ( 13 ). In this manner, Raman scattering light observation and CARS light observation can be selectively performed without moving the sample, so that the vibration frequency for the CARS light observation can be efficiently selected without needing complicated work.

Method for analyzing biological specimens by spectral imaging

A method for analyzing biological specimens by spectral imaging to provide a medical diagnosis includes obtaining spectral and visual images of biological specimens and registering the images to detect cell abnormalities, pre-cancerous cells, and cancerous cells. This method eliminates the bias and unreliability of diagnoses that is inherent in standard histopathological and other spectral methods. In addition, a method for correcting confounding spectral contributions that are frequently observed in microscopically acquired infrared spectra of cells and tissue includes performing a phase correction on the spectral data. This phase correction method may be used to correct various types of absorption spectra that are contaminated by reflective components.

Minimally invasive cytometry system with qcl inspection of single cells for cancer detection

This disclosure concerns a minimally invasive cytometry system including a handling system that presents single cells to at least one QCL laser source. The QCL laser source is configured to deliver light to a cell within the cells in order to induce vibrational bond absorption in one or more analytes within the cell. The cytometry system also includes a detection facility that detects the mid-infrared wavelength light transmitted by the cell and identifies the cell as either cancerous or non-cancerous.

Method for detecting a resonant nonlinear optical signal and device for implementing said method

The invention relates to a method and a device ( 100 ) for detecting a resonant non-linear optical signal induced in a sample ( 105 ), the sample comprising a resonant medium ( 41 ) and a non-resonant medium ( 42 ) forming an interface ( 43 ), the device comprising: an emission source ( 101 ) of at least one first excitation light beam, called a pump beam, at a first given angular frequency ω p for the excitation of the resonant medium of said sample, an optical detection module ( 106 ) for detecting a non-linear optical signal resulting from the interaction of said pump beam with an axial interface between the resonant and the non-resonant media of the sample, in at least two symmetrical directions ( , ′) relative to the optical axis of said excitation beam incident in the sample, and a processing unit ( 125 ) for processing signals (I Fwd ( ), I Fwd (

Method for investigating a sample using cars microscopy

A method for investigating a sample ( 1 ), derived from a biological source, using CARS microscopy is proposed, in which method a resonance signal generated by coherent anti-Stokes Raman scattering by excitation of at least one resonance site ( 5 ) of the sample ( 1 ) by means of laser irradiation is sensed in image-producing fashion. The method according to the present invention encompasses furnishing at least one resonance site ( 5 ) by means of a bioorthogonal reaction of an intrinsic chemical structure ( 2 ) of the sample ( 1 ) with at least one reaction partner ( 3, 6 ).

MEASUREMENT DEVICE AND MEASUREMENT METHOD

A measurement device includes: a light source that generates a pump beam and a Stokes beam; a pulse stretch section that stretches the pulse of the pump beam so that the pulse width of the Stokes beam is shorter than the pulse width of the pump beam; an optical splitter that splits the Stokes beam into two beams; an optical scan section that scans a subject with the pulse-stretched pump beam and one of the two beams split by the optical splitter; a first optical detector that detects an anti-Stokes beam from the subject; a second optical detector that detects an interference beam of the other of the two beams and the Stokes beam reflected by the subject; and a signal processing section that performs an image generation process based on a detection signal from the first optical detector and a detection signal from the second optical detector. 1. A measurement device comprising:a light source section that generates a pump beam and a Stokes beam that are applied to a subject;a pulse stretch section that stretches a pulse of the pump beam so that a pulse width of the Stokes beam is shorter than a pulse width of the pump beam;an optical splitter section that splits the Stokes beam into two beams;an optical scan section that scans the subject with the pulse-stretched pump beam and one of the two beams split by the optical splitter section;a first optical detection section that detects an anti-Stokes beam from the subject, and outputs a detection signal;a second optical detection section that detects an interference beam of the other of the two beams split by the optical splitter section and the Stokes beam reflected by the subject, and outputs a detection signal; anda signal processing section that performs an image generation process based on the detection signal from the first optical detection section and the detection signal from the second optical detection section.2. The measurement device as defined in claim 1 ,wherein the pulse-stretched pump beam is a picosecond ...

DEVICE FOR GENERATING A POLYCHROMATIC PHOTON BEAM HAVING SUBSTANTIALLY CONSTANT ENERGY

Some embodiments relate to a generation device that includes: a pulsed laser source generating primary photons having at least one wavelength within pulses having time dissymmetry, a forming device(s) controlling the primary photons so as to generate a selective-polarization, focused input beam, and an optical fiber wherein the primary photons induce secondary photons having different wavelengths resulting from a raman conversion cascade and forming a wide-spectrum output beam having substantially constant energy. 1. A device for generating a polychromatic beam of photons , comprising:a pulsed laser source that is able to deliver primary photons having a wavelength, a forming device able to act on the primary photons to deliver a focused input beam having a chosen polarization,an optical fiber that is arranged to produce, from the input beam, a polychromatic output beam including secondary photons having a plurality of wavelengths, wherein the pulsed laser source is able to deliver the primary photons in pulses having a temporal asymmetry such as to induce secondary photons having various wavelengths, resulting from a cascade of Raman conversions, and forming a broad-spectrum output beam having an energy that is distributed substantially constantly.2. The device as claimed in claim 1 , wherein the pulsed laser source is able to deliver a power density per meter of optical fiber that induces a non-linear generation of the secondary photons that is spread over time depending on their respective wavelengths.3. The device as claimed in claim 2 , wherein the power density per meter of optical fiber is higher than 0.2 kW/μm/m.4. The device as claimed in claim 2 , further comprising a filter installed downstream of optical fiber and that are able to let pass only secondary photons having wavelengths included in a predefined interval claim 2 , in order to deliver a filtered output beam taking the form of pulses of a duration equal to their difference in spread over time.5. ...

DEVICE FOR GENERATING A POLYCHROMATIC AND SPATIALLY SELF-ADAPTED BEAM OF PHOTONS

Some embodiments are directed to a generating device, including a pulse laser source providing primary photons having at least one wavelength, shaping device(s) acting on the primary photons to provide an input beam, a nonlinear crystal, and controller(s) generating, in the nonlinear crystal, at least one electric field that is synchronous with the input beam and suitable for inducing a phase mismatching in the nonlinear crystal through an electro-optical effect, in order to convert the primary photons of the input beam into secondary photons having wavelengths belonging to a supercontinuum. 1. A device for generating a polychromatic beam of photons , comprising:a pulsed laser source that is able to deliver primary photons having at least one wavelength,a forming device able to act on the primary photons to deliver an input beam,a non-linear crystal that is arranged to produce, from the input beam, a polychromatic output beam including secondary photons having a plurality of wavelengths, anda controller arranged to generate, in the non-linear crystal, at least one electric field that is synchronous with the input beam and able to induce, in the latter, a phase mismatch via an electro-optical effect in order to convert the primary photons of the input beam into secondary photons having wavelengths belonging to a super-continuum.2. The device as claimed in claim 1 , wherein the controller is arranged to generate claim 1 , in the nonlinear crystal claim 1 , a synchronous electric field in a first direction that is perpendicular to a general direction of the input and output beams and/or another synchronous electric field in a second direction that is perpendicular to a general direction of the input and output beams and to the first direction.3. The device as claimed in claim 1 , wherein the controller includes i) a sampler able to split the input beam into a main portion that is directed toward the nonlinear crystal and an auxiliary portion claim 1 , ii) an ...

Optical measurement device and optical measurement method

In multiplex CARS, a fingerprint region is a region where signals are densely concentrated. Information about the intensities and spatial distribution of these signals is important in cell analysis. However, there has been a problem in that the signal intensities are low. Accordingly, mechanisms 702 and 703 for adjusting the power branching ratio between light that enters a photonic crystal fiber 705 and pumping light; mechanisms 710 and 711 for adjusting the divergence/convergence state of the pumping light; and mechanisms 706 and 2101 for adjusting the divergence/convergence state of Stokes light are provided to enable adjustment for emphasizing a desired wavelength band.

WAFER INSPECTION APPARATUS AND SYSTEM INCLUDING THE SAME

A wafer inspection apparatus includes: an objective lens on an optical path of first and second input beams; and an image sensor configured to generate an image of the wafer based on scattered light according to a nonlinear optical phenomenon based on the first and second input beams, wherein the first input beam passing through the objective lens is obliquely incident on the wafer at a first incident angle with respect to a vertical line that is normal to an upper surface of the wafer, the second input beam passing through the objective lens is incident on the wafer at a second incident angle oblique to the vertical line that is normal to the upper surface of the wafer, and the first and second incident angles are different from each other. 1. A wafer inspection apparatus comprising:a stage configured to support a wafer;a first laser device configured to emit a first input beam to the wafer;a second laser device configured to emit a second input beam to the wafer;an objective lens on an optical path of the first and second input beams; andan image sensor configured to generate an image of the wafer based on scattered light according to a nonlinear optical phenomenon of the first and second input beams,wherein the first input beam passing through the objective lens is obliquely incident on the wafer at a first incident angle with respect to a vertical line that is normal to an upper surface of the wafer,the second input beam passing through the objective lens is incident on the wafer at a second incident angle oblique to the vertical line that is normal to the upper surface of the wafer, andthe first and second incident angles are different from each other.2. The wafer inspection apparatus of claim 1 , wherein no pattern is formed on the wafer.3. The wafer inspection apparatus of claim 2 , wherein the wafer is any one of a fused silica wafer claim 2 , a silicon wafer on which a SiOthin film is formed claim 2 , a silicon-on-insulator (SOI) wafer claim 2 , a SiNwafer ...

SYSTEMS AND METHODS FOR IMAGING AND PROCESSING TISSUE

In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue, capturing the sectioned tissue, and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, and capturing each sectioned tissue, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest. Further, captured tissue sections can be processed, re-imaged, and indexed to their original location in the three dimensional image. 1. A method for automated imaging and storage of tissue samples comprising:illuminating a first region of tissue with light using a multiphoton imaging microscope;detecting light from the tissue in response to the illuminating light to form an image of the first region of tissue;sectioning a portion of the imaged tissue to expose a second region of tissue;transporting the first portion of tissue;illuminating the second region of tissue;detecting light from the second region of tissue to form an image of the second region of tissue;sectioning a second portion from the second region of tissue;transporting the second portion of tissue;andfurther processing at least one sectioned tissue sample to generate processed tissue data;analyzing the processed tissue data in combination with the images of the first region of tissue and the second region of tissue.2. The method of wherein the analyzing step further comprises indexing the processed tissue images ...

Optical head and measuring apparatus

An optical head includes a first module that concentrates pump light and Stokes light on a first point; a second module that collects CARS light from the first point; and a third module that supports the first module and the second module. The first module includes: a high rigidity first frame; and a first optical system including a plurality of optical elements fixed to the first frame. The second module includes: a high rigidity second frame; and a second optical system including a plurality of optical elements fixed to the second frame. The third module includes a high rigidity third frame that fixes the first frame and the second frame.

Hybrid optical system

An optical system comprises a first optical path configured to supply a first light with a first range of wavelengths; a second optical path configured to supply a second light with a second range of wavelengths shorter than the first range of wavelengths; a third optical path configured to supply a third light with a third range of wavelengths shorter than the second range of wavelengths; an optical I/O unit configured to emit the first light, the second light and the third light to a target and acquire a light from the target; a reference unit configured to split off a reference light from the third light; and a detector that includes a range of detection wavelengths shared with a CARS light and an interference light.

Frequency Comb for Downhole Chemical Sensing

The present disclosure relates to systems and methods for analyzing fluids. The method for analyzing a chemical sample within a wellbore, contained within an interrogation device, may comprise broadcasting a coherent light from a frequency comb module, directing the coherent light through a fiber optic line to the interrogation device, irradiating the chemical sample with the coherent light, capturing light resulting from the irradiation of the chemical sample, and producing a spectrum resulting from the captured light from the chemical sample. A frequency comb system for analyzing a chemical sample may comprise a frequency comb module configured to broadcast a coherent light and a fiber optic line that extends into a wellbore to an interrogation device. The interrogation device may further be configured to contain the chemical sample for irradiation by the coherent light. The frequency comb system may further comprise a receiver and an information handling system. 1. A method for analyzing a chemical sample within a wellbore , contained within an interrogation device , the method comprising:broadcasting a coherent light from a frequency comb module;directing the coherent light through a fiber optic line to the interrogation device;irradiating the chemical sample with the coherent light;capturing light resulting from the irradiation of the chemical sample; andproducing a spectrum resulting from the captured light from the chemical sample.2. The method of further comprising capturing the chemical sample in the interrogation device in the wellbore.3. The method of claim 1 , wherein the fiber optic line extends into the wellbore.4. The method of claim 1 , wherein the frequency comb module comprises at least one frequency comb.5. The method of claim 1 , wherein the frequency comb module comprises a beam splitter and a low pass filter.6. The method of claim 1 , further comprising altering the irradiated light from the frequency comb module with an information handling ...

SUPER-RESOLUTION OBSERVATION DEVICE AND SUPER-RESOLUTION OBSERVATION METHOD

A super-resolution observation device includes an illumination optical system collecting a first illuminating light having a first optical frequency co, on a first region of an observation object, collecting a second illuminating light having a second optical frequency ω′ on a second region partially overlapping the first region, and collecting a third illuminating light having a third optical frequency ωon a third region containing a non-overlap region which is a region of the first region and does not overlap the second region; and an extraction unit extracting a signal light generated in accordance with a change in an energy level of a substance in the non-overlap region from a light generated in all of the first region, the second region, and the third region. 1. A super-resolution observation device , comprising:{'sub': 1', '2', '2, 'an illumination optical system collecting a first illuminating light having a first optical frequency ωon a first region of an observation object, collecting a second illuminating light having a second optical frequency ω′ on a second region partially overlapping the first region, and collecting a third illuminating light having a third optical frequency ωon a third region containing a non-overlap region which is a region of the first region and does not overlap the second region; and'}an extraction unit extracting a signal light generated in accordance with a change in an energy level of a substance in the non-overlap region from a light generated in all of the first region, the second region, and the third region.2. The super-resolution observation device according to claim 1 , wherein:the illumination optical system sequentially emits the first illuminating light, the second illuminating light, and the third illuminating light to the observation object; and{'sub': '2', 'the extraction unit extracts the signal light having an optical frequency ω.'}3. The super-resolution observation device according to claim 2 , wherein the ...

Cell observation method, cell observation apparatus, readable media, method for producing cell sheet and apparatus for producing cell sheet

A cell observation method includes a detection step of detecting radiation light that is radiated from an observation target when the observation target is irradiated with irradiation light; and a determination step of determining whether a cell included in the observation target is alive or dead based on the radiation light. Another cell observation method may include detecting radiation light that is radiated from an observation target when the observation target is irradiated with irradiation light; and detecting the number of live cells included in the observation target based on the radiation light. Another cell observation method may include acquiring a spectrum of irradiation light that irradiates an observation target when the observation target is irradiated with the irradiation light; and detecting a state of at least one of lipid and protein included in the observation target based on the spectrum.

NANOPARTICLES IN THE SHAPE OF NANOSNOWMAN WITH A HEAD PART AND A BODY PART, A PREPARATION METHOD THEREOF AND A DETECTION METHOD USING THE SAME

The present invention relates to nanoparticles in the shape of nanosnowman with a head part and a body part, a preparation method thereof, and a detection method using the same. More particularly, the present invention relates to nanoparticles in the shape of nanosnowman with head and body parts, which can offer platforms for DNA-based assembly of various aligned and unconventional nanostructures and is highly applicable to the detection of DNA and an analyte associated, with the onset and progression of a particular disease, a preparation method thereof, and a detection method using the same. 1. A method for preparing snowman-shaped nanoparticles , which comprises a gold or silver nanoparticle head part , a gold , or silver nanoparticle body part , and a plurality of oligonucleotides bound to the surface of the head part , wherein a portion of the head part is located on a concave region in a portion of the body part , and a portion of the oligonucleotides bound to the surface of the head part are buried , in the concave region in the body part , and the remaining portion of the oligonucleotides are exposed on the surface of the head part ,comprising the following steps of:1) modifying a gold or silver nanoparticle with oligonucleotides (step 1); and2) reacting the oligonucleotide-modified gold or silver nanoparticle with a gold or silver precursor in the presence of NaCl at a concentration of 1 nM to 0.1 M, a reducing agent, and a stabilizer (step 2).2. The method according to claim 1 , wherein the gold or silver precursor is AgNO claim 1 , AgClOor HAuCl.3. The method according to claim 1 , wherein step 2) is performed under the condition of pH 2 to 7.4. The method according to claim 1 , wherein the reducing agent is hydroquinone claim 1 , sodium borohydride (NaBH) claim 1 , sodium ascorbate claim 1 , hydroxyl amine or a combination thereof.5. The method according to claim 1 , wherein the stabilizer is a derivative including pyrrolidine claim 1 , imidazolidine ...

SECOND AND THIRD ORDER SIMULTANEOUSLY NON-LINEAR OPTICAL PROCESSES AND MEASUREMENTS FOR SURFACE ANALYSIS

Methods, systems and apparatuses are disclosed for interrogating characteristics of a substrate material surface and sub-surface by evaluating Terahertz output signals generated by non-Terahertz, optical source inputs. 1. A method for non-invasively evaluating a substrate material comprising the steps ofsubstantially simultaneously directing a first input beam of radiation having a first frequency from a first optical source and a second input beam of radiation having a second frequency from a second optical source to a predetermined area on a substrate material surface, with the first frequency differing from the second frequency by a predetermined amount;mixing the first input beam and the second input beam at the substrate material surface;producing second order and third order output signals in the THz regime from the substrate material;directing the second order output signal to a second order output signal detector to receive the second order output signal from the substrate material;directing the third order output signal to a third order output signal detector to receive the third order output signal from the substrate material; andmeasuring at least one characteristic of the substrate material based on the second output signal and third output signal received by the second order output signal detector and the third order output signal detector.2. The method of further comprising the steps of:directing a first/second-harmonic output signal to a first/second output signal detector positioned to receive a first/second-harmonic output signal from the first optical source;directing a second/second-harmonic output signal to a second/second-harmonic output detector positioned to receive a second/second-harmonic output signal from the second optical source; andmeasuring at least one characteristic of the substrate material based on the first/second-harmonic output signal received by the first/second-harmonic output signal detector and the second/second-harmonic ...

COHERENT ANTI-STOKES RAMAN SCATTERING MICROSCOPE IMAGING APPARATUS

A coherent anti-Stokes Raman scattering microscope imaging apparatus, comprising: a laser light source (), a two-dimensional oscillating mirror assembly (), a first light dichroic mirror plate (), an objective lens (), a sample translation platform (), a collection device (), and a data processing module; the laser light source () is used for producing a first laser beam and a second laser beam; the first laser beam and the second laser beam are coaxially emitted; the first laser beam and the second laser beam are incident on the two-dimensional oscillating mirror assembly (), and the two-dimensional oscillating mirror assembly () adjusts the optical path of the first laser beam and the second laser beam; the first laser beam and the second laser beam leaving the two-dimensional oscillating mirror assembly pass in sequence through the first light dichroic mirror plate () and the objective lens (); the objective lens () focuses the first laser beam and the second laser beam onto the sample translation platform; the signal light produced on the sample translation platform () passes through the objective lens (), and the collection device () produces initial data on the basis of the signal light, and outputs the initial data to the data processing module; the need for beam splitting and wavelength adjustment of a single wavelength laser beam outputted by a laser is thereby avoided. 1. A coherent anti-Stokes Raman scattering microscope imaging device , comprising: a laser light source , a two-dimensional galvanometer assembly , a first dichroic mirror , an objective lens , a sample translation platform , a collector , and a data processing module; wherein the laser light source is configured to generate a first laser beam and a second laser beam; the first laser beam and the second laser beam are output collinearly; the first laser beam and the second laser beam are incident on the two-dimensional galvanometer assembly , the two-dimensional galvanometer assembly adjusts ...

Spectral microscopy device

A spectral microscopy device includes a spectral detecting unit including a light source that is capable of controlling an output wavelength, a microscope section that is provided with an observation area that is illuminated with light output from the light source, and a signal detector that detects light from the observation area as spectral data; a moving unit configured to move the observation area; and a controller that performs a control operation to allow the spectral detecting unit and the moving unit to move in response to each other. The spectral microscopy device is controlled so that switching between different measurement conditions is performed at an observation area movement time in which the observation area is moved by the moving unit and measurement is performed and at an observation area movement stoppage time in which the observation area is fixed and measurement is performed.

Test object visualizing device

Provided is a test object visualizing device, including: light irradiating unit configured to make wavelength of at least any one of pump light and Stokes light generated on the same optical path variable per test position of test object, and irradiate test object with pump light and Stokes light; molecule distribution image generating unit configured to detect anti-Stokes light emitted from test object according to wavelength difference between pump light and Stokes light, and generate a molecule distribution image based on anti-Stokes light; tomographic image generating unit configured to detect at least any one of reflected light from test object when irradiated with pump light and reflected light from test object when irradiated with Stokes light, and generate a tomographic image of test object based on the reflected light detected; and image display unit configured to display at least any one of molecule distribution image and tomographic image generated.

DETECTION SYSTEMS AND METHODS USING COHERENT ANTI-STOKES RAMAN SPECTROSCOPY

Systems and methods for remote and/or portable detection are provided. The system can include a source of coherent laser pulses, components for converting the coherent laser pulses into first beam pulses at a first wavelength value, second beam pulses at a second wavelength value, and third beam pulses at a third wavelength value. Systems can further include optical components configured to delay at least one of the first beam pulses, the second beam pulses, and the third beam pulses in order to create delayed beam pulses, and a focusing component configured direct a substantially collinear combination of the delayed beam pulses and two of a set of: the first beam pulses, the second beam pulses, and the third beam pulses, onto a sample. 1. A system for remote detection , the system comprising:a source of coherent laser pulses;a parametric amplifier configured to convert the coherent laser pulses into first beam pulses at a first wavelength value and second beam pulses at a second wavelength value different from the first wavelength value;a first optical component configured to select third beam pulses from the coherent laser pulses at a third wavelength value different from the second wavelength value and the first wavelength value;a second optical component configured to delay at least one of the first beam pulses, the second beam pulses, and the third beam pulses in order to create delayed beam pulses; anda focusing component configured direct a substantially collinear combination of the delayed beam pulses and two of a set of: the first beam pulses, the second beam pulses, and the third beam pulses, onto a line, the line being substantially perpendicular to a direction of propagation of the collinear combination.2. The system of claim 1 , wherein the at least one of the first beam pulses claim 1 , the second beam pulses and the third beam pulses are the third beam pulses claim 1 , and wherein the first beam pulses are pump beam pulses claim 1 , the second beam ...

SURFACE-FUNCTIONALIZED NANOSTRUCTURES FOR MOLECULAR SENSING APPLICATIONS

Surface-functionalized nano structures, arrays of the nanostructures, and method for using the arrays in surfaced-enhanced spectroscopy and dielectric sensing applications, such as surface-enhanced infrared absorption spectroscopy, are provided. The nanostructures are functionalized with specific binding moieties that are bound to the nanostructures via phosphonic acid linkers. 1. A substrate for surface-enhanced spectroscopy comprising(a) a substrate; and(b) an array of nanostructures on the substrate, wherein the nanostructures are functionalized with specific binding moieties that are bound to the nanostructures via phosphonic acid linkers,wherein the nanostructures comprise an electrically conductive oxide, an electrically conductive nitride, an electrically conductive boride, an electrically conductive carbide, or a non-noble metal.2. The substrate of claim 1 , wherein phosphonic acid linkers comprise the structure —R—P(OH) claim 1 , wherein R represents a single bond or a spacer comprising at least a carbon and hydrogen.3. The substrate of claim 2 , wherein R is an alkyl group claim 2 , an alkoxyl group or a thioalkyl group.4. The substrate of claim 2 , wherein specific binding moieties are bound to the R groups of the phosphonic acid linkers.5. The substrate of claim 2 , wherein specific binding moieties are bound directly to the P atoms of the phosphonic acid linkers.6. The substrate of claim 1 , wherein the nanostructures comprise electrically conductive metal oxide nanoparticles.7. The substrate of claim 6 , wherein the nanostructures comprise tin oxide claim 6 , indium-tin-oxide claim 6 , doped titanium oxide claim 6 , or doped zinc oxide.8. The substrate of claim 1 , wherein the nanostructures have a native surface oxide layer and the phosphonic acid linkers are bound to the native surface oxide.9. The substrate of claim 8 , wherein the nanostructures are aluminum nanostructures and the surface oxide is aluminum oxide.10. The substrate of claim 1 , ...

LASER BASED APPARATUS, METHODS AND APPLICATIONS

Embodied is a two-color, fiber-delivered picosecond source for coherent Raman scattering (CRS) imaging. A wavelength tunable picosecond pump is generated by nonlinear spectral compression of a prechirped femtosecond pulse from a mode-locked titanium:sapphire (Ti:S) laser. A 1064-nm picosecond Stokes pulse is generated by an all-fiber time-lens source (or suitable alternative source) that is synchronized to the Ti:S laser. The pump and Stokes beams are combined in an optical fiber coupler, which serves not only as the delivery fiber but also as the nonlinear medium for spectral compression of the femtosecond pulse. CRS imaging of mouse skin is performed to demonstrate the practicality of this source. 1. An optical apparatus comprising:a first laser source that provides a first laser output through a first laser output port;a prechirping apparatus optically coupled to the first laser output port to provide a prechirped first laser output from the first laser output; and a second laser source different than the first laser source; and', 'a time-lens source., 'a second light source synchronized with the first laser source and having a second light output that is optically coupled with the prechirped first laser output, the second light source selected from the group consisting of2. The optical apparatus of wherein the first laser source comprises a mode-locked laser source.3. The optical apparatus of wherein the second laser source comprises a continuous wave laser source.4. The optical apparatus of wherein the second light source comprises an all-fiber time-lens source.5. An optical apparatus comprising:a laser source that provides a laser output through a laser output port;a prechirping apparatus optically coupled to the laser output port to provide a prechirped laser output from the laser output;a time-lens source synchronized with the laser source and having a time-lens output that is optically coupled with the prechirped laser output.6. The optical apparatus of ...

LOW-ABERRATION HIGH-SPEED-COMPATIBLE OPTICAL DELAY LINES AND METHODS THEREOF

This disclosure describes an example architecture for providing a delay line for optical techniques. The delay line architecture includes a focusing element that has a focal axis disposed parallel to its length. The line of symmetry provided by the focal axis obviates path-length-dependent aberrations caused by the off-axis beam translations. The systems described herein also provide varying geometries of movable mirrors, including a galvanometer mirror and a rotating polygonal mirror. The systems and methods described herein also provide techniques for generating and detecting coherent Raman spectra using a picosecond probe pulse. 1. A method comprising:directing combined light fields across an object of interest thereby creating one or more new light fields with new spectral frequency content;directing one or more of the new light fields onto one or more detectors after transmission through or scattering from the object of interest; anddetermining a frequency-dependent phase and/or amplitude of one or more of the new light fields based on a delay between light fields of the combined light fields.2. The method of further comprising:directing a first light field to a delay module; anddelaying the first light field via the delay module to create a delayed light field;wherein the combined light fields comprise the delayed light field and a second light field.3. The method of claim 1 , wherein: a movable mirror;', 'a focusing optical element having a focal axis parallel to its length; and', 'a return mirror;, 'the delay module comprisesthe movable mirror is configured to receive the first light field and reflect the first light field to the focusing optical element;the focusing optical element is configured to receive the first light field reflected from the movable mirror and return the first light field to the return mirror;the return mirror is configured to receive the first light field reflected from the focusing optical element and reflect the first light field ...

OPTICAL ANALYSIS DEVICE

Spectral data such as a CARS spectrum of a sample is acquired at high speed by reducing the amount of data. During scan by emission light focused and emitted onto the sample, the exposed state of a detection unit of a spectroscope that divides light generated from the sample is continued, thereby acquiring spectral data obtained by summing spectra generated at a plurality of positions in the sample. 1. An optical analyzing apparatus comprising:a light source;a sample holding unit that holds a sample;an emission optical system that focuses and emits a light flux from the light source onto the sample held by the sample holding unit;a light division unit that divides light generated from the sample by light emission;a detection unit that detects the light divided by the light division unit; andan emission control unit that controls the position of light emission onto the sample by the emission optical system,wherein the detection unit continues an exposed state over a plurality of positions of light emission onto the sample by the emission control unit, and outputs a spectrum obtained by summing spectra generated from the positions of light emission.2. The optical analyzing apparatus according to claim 1 ,wherein the detection unit outputs a plurality of the summed spectra, and averages the plurality of outputted spectra.3. The optical analyzing apparatus according to claim 1 , further comprising:an image data acquisition unit that acquires image data of the sample held by the sample holding unit; anda shape recognition unit that recognizes the shape of the sample based on the acquired image data,wherein the emission control unit focuses and emits a light flux from the light source onto a specific region of the sample based on the shape of the sample recognized by the shape recognition unit.4. The optical analyzing apparatus according to claim 1 ,wherein the spectrum is a CARS spectrum.5. The optical analyzing apparatus according to claim 1 ,wherein the emission ...

System and method for performing a photoluminescence analysis on a medium

Systems and methods for performing a photoluminescence analysis on a medium such as the fundus of a patient's eye are provided. An imaging device, a spectral analyser and an excitation light source are provided. An optical assembly defines an imaging light path between the patient's eye and the imaging device and a spectral analysis light path between an analysis spot on the patient's eye and the spectral analyser. The excitation light source generates an excitation light beam comprising an excitation wavelength selected to excite components present in the patient's eye for the generation of fluorescent light at a photoluminescence wavelength, and is optically coupled to the spectral analysis light path for projecting the excitation light beam on the analysis spot. An optical filter is coupled to the spectral analyser, and has a low light transmissivity at the excitation wavelength and a high light transmissivity at the photoluminescence wavelength.

DEVICES, APPARATUS AND METHOD FOR PROVIDING PHOTOSTIMULATION AND IMAGING OF STRUCTURES

According to exemplary embodiments of the present disclosure, it is possible to provide method, system, arrangement, computer-accessible medium and device to stimulate individual neurons in brain slices in any arbitrary spatio-temporal pattern, using two-photon uncaging of photo-sensitive compounds such as MNI-glutamate and/or RuBi-Glutamate with beam multiplexing. Such exemplary method and device can have single-cell and three-dimensional precision. For example, by sequentially stimulating up to a thousand potential presynaptic neurons, it is possible to generate detailed functional maps of inputs to a cell. In addition, it is possible to combine this exemplary approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity. Further exemplary embodiments of the present disclosure can include a light-weight, compact portable device providing for uses in a wide variety of applications. 151-. (canceled)52. A device for affecting at least one radiation and imaging neuronal circuits , comprising: affect a user-specified three-dimensional spatial profile of the at least one radiation;', 'split the at least one radiation into multiple beamlets;', 'combine the beamlets using at least one galvanometer mirror; and', 'trigger at least one of a photo activation, a photo-inactivation or a photo-chemical effect of at least one portion of at least one sample by exciting the at least one portion in a non-linear manner using the beamlets., 'at least one spatial light modulator (SLM) arrangement which is structured or configured to53. The device according to any of claim 52 , wherein the at least one sample is at least one of a biological sample claim 52 , a chemical composition claim 52 , a semiconductor arrangement or a drug-delivery arrangement.54. The device according to claim 52 , wherein the at least one SLM arrangement includes at least one diffractive optical arrangement.55. The device according to claim 52 , wherein the at least ...

METHOD FOR ANALYZING BIOLOGICAL SPECIMENS BY SPECTRAL IMAGING

A method for analyzing biological specimens by spectral imaging to provide a medical diagnosis includes obtaining spectral and visual images of biological specimens and registering the images to detect cell abnormalities, pre-cancerous cells, and cancerous cells. This method eliminates the bias and unreliability of diagnoses that is inherent in standard histopathological and other spectral methods. In addition, a method for correcting confounding spectral contributions that are frequently observed in microscopically acquired infrared spectra of cells and tissue includes performing a phase correction on the spectral data. This phase correction method may be used to correct various types of absorption spectra that are contaminated by reflective components. 1. A method executed by a system for analyzing biological specimens by spectral imaging , comprising:acquiring a visual image of the biological specimen;acquiring a spectral image of a biological specimen that includes spectral data from the biological specimen;performing multivariate analysis of the spectral data to detect spectral differences in the spectral data;generating a grayscale or pseudo-color image based at least upon the multivariate analysis; andregistering the visual image and the grayscale or pseudo-color spectral image to generate a registered image.2. The method of claim 1 , further comprising:detecting cell abnormalities in the biological specimen using the registered image.3. The method of claim 1 , wherein the multivariate analysis of the spectral data comprises one or more of performing unsupervised analysis of the spectral data and performing analysis of the spectral data using a supervised machine learning algorithm. This application is a continuation of U.S. patent application Ser. No. 14/606,812, filed on Jan. 27, 2015, which is a continuation of U.S. Patent Application No. 13/067,777, filed on Jun. 24, 2011, which claims the benefit of U.S. Provisional Patent Application No. 61/358,606, ...

Optical Arrangement for a Spectroscopic Imaging Method and Spectroscopic Imaging Method

In an embodiment an optical arrangement includes a multicore fiber having at least a first fiber core configured to guide a first illumination light and a second fiber core configured to guide a second illumination light, wherein the multicore fiber comprises a fiber scanner configured to deflect the multicore fiber or the multicore fiber is followed by a mirror scanner; and a wavelength dispersive beam combiner configured to spatially superimpose the first illumination light and the second illumination light in an object space.

Apparatus, a Handheld Electronic Device, and a Method for Carrying Out Raman Spectroscopy

An apparatus, a handheld electronic device and a method for carrying out Raman Spectroscopy are disclosed. In an embodiment an apparatus includes at least one optoelectronic laser configured to provide excitation radiation to a sample, the excitation radiation being generated by an electric current flowing through the at least one optoelectronic laser during operation of the apparatus and a transistor configured to modulate the electric current flowing through the at least one optoelectronic laser, to thereby switch on and off generation of the excitation radiation. 1. An apparatus for carrying out Raman spectroscopy on a sample , the apparatus comprising:at least one optoelectronic laser configured to provide excitation radiation to the sample, the excitation radiation being generated by an electric current flowing through the at least one optoelectronic laser during operation of the apparatus; anda transistor configured to modulate the electric current flowing through the at least one optoelectronic laser, to thereby switch on and off generation of the excitation radiation.2. The apparatus of claim 1 , wherein the at least one optoelectronic laser is a DFB laser diode or DBR laser diode.3. The apparatus of claim 1 , wherein the transistor comprises an electric contact and the optoelectronic laser comprises an electric contact claim 1 , and wherein the electric contact of the transistor is directly coupled electrically to the electric contact of the optoelectronic laser.4. The apparatus of claim 1 , wherein the transistor is a GaN FET configured to operate the at least one optoelectronic laser such as to generate pulsed excitation radiation.5. The apparatus of claim 1 , wherein the at least one optoelectronic laser is configured to generate pulses of excitation light claim 1 , wherein each pulse has a time duration of less than 200 ps.6. The apparatus of claim 1 , further comprising a temperature sensor configured to monitor a temperature of the at least one ...

Optical Sources

A coherent anti-stokes Raman scattering apparatus for imaging a sample comprises an optical output; an optical source arranged to generate a first optical signal at a first wavelength; and a nonlinear element arranged to receive the first optical signal, where the nonlinear element is arranged to cause the first optical signal to undergo four-wave mixing on transmission through the nonlinear element such that a second optical signal at a second wavelength and a third optical signal at a third wavelength are generated, wherein an optical signal pair comprising two of the first, second and third optical signals is provided to the optical output for imaging the sample. 1an optical output;an optical source arranged to generate a first optical signal at a first wavelength;a nonlinear element arranged to receive the first optical signal, the nonlinear element being arranged to cause the first optical signal to undergo four-wave mixing on transmission through the nonlinear element such that a second optical signal at a second wavelength and a third optical signal at a third wavelength are generated, wherein an optical signal pair comprising two of the first, second and third optical signals is provided to the optical output for imaging the sample.. A coherent anti-stokes Raman scattering apparatus for imaging a sample, comprising: The present application is a continuation of and claims priority to application Ser. No. 14/337,169, filed 21 Jul. 2014 and entitled “Optical Sources”. application Ser. No. 14/337,169 claims priority to and is a continuation of application Ser. No. 13/522,594, filed Jul. 17, 2012, entitled “Optical Sources” and now issued as U.S. Pat. No. 8,785,884. application Ser. No. 13/522,594 claims priority to International PCT Patent Application PCT/GB2011/050106, bearing an International Filing Date of 24 Jan. 2011 and also entitled “Optical Sources.” PCT/GB2011/050106 claims priority to Patent Application GB 1001051.0, filed 22 Jan. 2010. The present ...

METHOD AND DEVICE FOR THE RAMAN SPECTROSCOPIC, IN OVO SEX DETERMINATION OF FERTILISED AND INCUBATED BIRDS' EGGS

The invention relates to a method for the Raman spectroscopic, in ovo sex determination of fertilised and hatched birds' eggs (), wherein the embryo, including the extra-embryonic structures, can move in the egg, and is not yet attached to the shell at the time of measuring. In addition, the following steps are carried out: monitoring the time course of the hatched egg until forming at least one recognisable blood vessel (); creating a hole () in the shell in the region near to the attached bloody vessel, using a hole-generating unit; finding the blood vessel forming in the egg, using a vision system () and a coaxial or lateral illumination with light () in the visible wavelength range; positioning at least one blood vessel in the laser focus of a laser source (), either by moving the egg or moving a lens () of a device () for introducing the laser light (), and detecting the Raman scattered radiation (); registering the Raman scattered radiation of the irradiated blood vessel using the device for introducing the laser light, and for detecting the Raman scattered radiation, wherein, during the measuring process, a movement of the blood vessel out of the focus can be avoided by tracking using the vision system; evaluating the Raman scattered radiation in an evaluation unit; determining and displaying the sex of the embryo in the bird's egg. 11231287. A method for the Raman spectroscopic in ovo sex determination of fertilized and incubated birds' eggs () , wherein the embryo () , including the extra-embryonic structures , can move in the egg () and is not yet attached to the shell () at the point in time of a measurement of the Raman scattered radiation () , [{'b': 21', '27, 'monitoring the chronological progression of the incubation until at least one identifiable blood vessel (, ) develops;'}, {'b': 2', '28', '21', '27', '29, 'creating a hole () in the shell () in the proximate region of the attached blood vessel (, ) by means of a hole-creating unit ();'}, {'b': 21 ...

Laser device

A laser device for outputting filtered light pulses for inducing coherent Raman scattering in a sample. The laser device comprises a first optical cavity comprising a first gain medium; and a second optical cavity comprising a second gain medium different to the first gain medium. The first gain medium and the second gain medium are each excitable by a pump light source to generate light at respective different ranges of wavelengths. A synchronizer is optically coupled to both the first optical cavity and the second optical cavity. The synchronizer is configured to synchronize and mode-lock light from the first optical cavity and the second optical cavity. The laser device also includes a first optical filter and a second optical filter. The first optical filter and the second optical filter are configured to filter the light from the first optical cavity and the second optical cavity respectively in order to output first filtered light pulses at a first predetermined range of wavelengths and second filtered light pulses at a second predetermined range of wavelengths.

Spectroscopy for gunshot residue analysis

The present invention relates to a method of detecting inorganic gunshot residue (GSR) particles. The method includes providing a sample comprising gunshot residue, subjecting the sample to spectroscopic analysis to produce a spectroscopic signature for the sample, and identifying inorganic gunshot residue particles based on the spectroscopic signature for the sample. Also disclosed is a method of detecting gunshot residue particles. The method includes providing a sample comprising gunshot residue, subjecting the sample to spectroscopic analysis to produce a spectroscopic signature for the sample, where the spectroscopic signature spans a range of wavenumbers, creating one or more spectroscopic maps from the spectroscopic signature for the sample, where each different spectroscopic map is for a different wavenumber, and identifying gunshot residue particles based on the one or more spectroscopic maps for the sample.

Innovative Nanopore Sequencing Technology

Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

Manipulating the translation of dna strands across and through nanopore sequencing systems using raman signatures to identify dna bases and methods

Nucleic acid sequencing methods and systems, the systems including nanochannel chip including: a nanochannel formed in an upper surface of the nanochannel chip and; a roof covering the nanochannel and comprising nanopores and a field enhancement structure; and a barrier disposed in the nanochannel. The method including: introducing a buffer solution including long-chain nucleic acids to the nanochannel chip; applying a voltage potential across the nanochannel chip to drive the nucleic acids through the nanochannel, towards the barrier, and to translocate the nucleic acids through nanopores adjacent to the barrier, such that bases of each of the nucleic acids pass through the field enhancement structure one base at a time and emerge onto an upper surface of the roof; detecting the Raman spectra of the bases of the nucleic acids as each base passes through the electromagnetic-field enhancement structure; and sequencing the nucleic acids based on the detected Raman spectra.

QUANTITATIVE NONLINEAR OPTICAL MICROSCOPY USING A SHAPED BEAM

A nonlinear optical microscope is provided, including source of a pulsed laser beam; a spatial light modulator for modulating the spatial profile of the pulsed laser beam; an objective for guiding the modulated beam towards a slide intended to carry a specimen; and a detector for collecting signals originating from the specimen, wherein the spatial light modulator is designed to modulate the intensity and/or the phase of the pulsed laser beam on the rear pupil of the objective to produce a beam that is axially extended and confined in one or two lateral directions after focusing by the objective, and wherein the slide is placed on a motorized stage of a histology slide scanner assembly. 1. A nonlinear optical microscope comprising:a source of a pulsed laser beam;a spatial light modulator for modulating the spatial profile of the pulsed laser beam:an objective for guiding the modulated beam towards a slide intended to carry a specimen; anddetection means for collecting signals originating from the specimen, wherein the spatial light modulator is designed to modulate the intensity and/or the phase of the pulsed laser beam on the rear pupil of the objective so as to produce a beam that is axially extended and confined in one or two lateral directions after focusing by the objective, and wherein the slide is placed on a motorized stage of a histology slide scanner assembly.2. The microscope according to claim 1 , characterized in that the modulated beam is a Bessel beam produced in the form of an annular intensity distribution on the rear pupil of the objective.3. The microscope according to claim 1 , characterized in that the slide scanner assembly comprises:a source of white light;a condenser for guiding the white light towards the slide; anda camera for producing an image from the light originating from the slide.4. The microscope according to any one of the claim 1 , characterized in that the detection means comprise a condenser and a detector arranged downstream of ...

CARS MICROSCOPE

A microscope includes: a first light dividing part that divides a light flux of light from a light source into a first pump light flux and a second pump light flux; a Stokes light source that receives the second pump light flux as an input and outputs a Stokes light flux; a multiplexing part that multiplexes the first pump light flux and the Stokes light flux to generate a multiplexed light flux; a first light-collecting part that collects the multiplexed light flux in a sample; a first detector that detects CARS light generated from the sample, the CARS light having a wavelength different from the multiplexed light flux; a second light dividing part that lets at least one of the second pump light flux and the Stokes light flux branch partially as a reference light flux; a second multiplexing part that multiplexes a light flux from the sample and the reference light flux to generate interfering light; and a second detector that detects the interfering light. 1. A CARS microscope , comprising:a light source;a first light dividing part that divides a light flux of output light from the light source into a first pump light flux and a second pump light flux;a Stokes light source that receives the second pump light flux as an input and outputs a Stokes light flux;a multiplexing part that multiplexes the first pump light flux and the Stokes light flux to generate a multiplexed light flux;a first light-collecting part that collects the multiplexed light flux in a sample;a first detector that detects CARS light generated from the sample, the CARS light having a wavelength different from the multiplexed light flux;a second light-collecting part that guides the CARS light to the first detector;a second light dividing part that lets at least one of the first pump light flux and the Stokes light flux partially branch as a reference light flux;a second multiplexing part that multiplexes a light flux from the sample and the reference light flux to generate interfering light; anda ...

Laser scanning method for measuring in vivo substances

In a laser scanning method for measuring in vivo substances, wavelength of the anti-Stokes line is made greater than wavelength absorption band of the specific substance, the wavelength of the probe light is shifted from the wavelength of the anti-Stokes line by a shift amount of Raman scattering of the specific substance, and the wavelength of the Stokes light is shifted from the wavelength of the probe light by the shift amount of the Raman scattering of the specific substance. According to this, density (concentration and distribution) of lutein in an eye fundus is measured quantitatively and non-invasively from a signal strength level of the anti-Stokes line. 1

DEVICES AND METHODS FOR DIRECT-SAMPLING ANALOG TIME-RESOLVED DETECTION

Devices and methods for sampling an analog signal to perform data analysis are disclosed. The sampling devices and corresponding methods include a detector module that measures a response generated from a sample, an analog to digital converter that samples the analog signal, received from the detector module, and converting it into a digital signal, a sampling rate of the converter being faster than the response of the sample; and a logic circuit coupled to the converter that processes the digital signal to generate a reduced digital data signal, the logic circuit processing the digital signal acquired from the converter to generate a continuous data transfer to a processing system. 1. A sampling device for measuring an analog signal from a detector comprising:a light detector module for measuring a response generated from a biological sample in response to illuminating light from a light source and generating an analog signal;an analog to digital converter to receive the analog signal from the detector module and convert it into a digital signal;a logic circuit coupled to the analog to digital converter that processes the digital signal; andwherein the logic circuit that processes the digital signal continuously transfers the processed digital signal to a data storage device.2. The sampling device of claim 1 , further comprising a memory device coupled to the logic circuit for buffering processed digital signals from the logic circuit claim 1 , the memory device further including a data processor.3. The sampling device of claim 2 , further comprising an interface coupled to the memory device for streaming the processed digital signals to an external display device.4. The sampling device of claim 1 , further comprising a reference clock coupled to the analog to digital converter for generating a sampling clock signal.5. The sampling device of claim 1 , further comprising a data clock coupled to the logic circuit for providing a reference clock pulse for processing ...

DEVICE AND METHOD FOR ILLUMINATING A SAMPLE

A device () for illuminating a sample () is described, having: at least one pulsed laser light source () for repeated emission of a first laser pulse along a first light path () and of a second laser pulse along a second light path () physically separated from the first light path; a superimposition element () for collinear superimposition of the two laser pulses in a shared light path (); a delay stage () arranged in the first or the second light path (), for delaying one of the two laser pulses relative to the other laser pulse in such a way that the two laser pulses sent along the shared light path () onto the sample () exhibit a temporal superimposition; a shared chirp unit () arranged in the shared light path (), for frequency-modifying influencing both of the first laser pulse and of the second laser pulse; and at least one separate chirp unit () arranged in the first light path (), for frequency-modifying influencing only of the first laser pulse. The shared chirp unit () and the separate chirp unit () are coordinated with one another in order to achieve a target state. The separate chirp unit () is coupled to a control system () by which the separate chirp unit () is controllable with a control parameter dependent on the wavelength of the first laser pulse in order to establish the target state. 1. A device for illuminating a sample , havingat least one pulsed laser light source for repeated emission of a first laser pulse along a first light path and of a second laser pulse along a second light path physically separated from the first light path;a superimposition element for collinear superimposition of the two laser pulses in a shared light path;a delay stage, arranged in the first or the second light path, for delaying one of the two laser pulses relative to the other laser pulse in such a way that the two laser pulses sent along the shared light path onto the sample exhibit a temporal superimposition;a shared chirp unit, arranged in the shared light path ...

REMOTE SUBSTANCE IDENTIFICATION DEVICE AND REMOTE SUBSTANCE IDENTIFICATION METHOD

Object: To provide a remote substance identification device that can identify an unidentified substance, such as a harmful substance, from a remote location. Solution: Provided are a remote substance identification device and method, the device comprising a laser device that emits a laser beam to an irradiated space; a wavelength conversion device that converts a wavelength of the laser beam emitted from the laser device into a plurality of different wavelengths and that emits laser beams of the different wavelengths to the irradiated space; a light collecting-detecting device that collects and detects resonance Raman-scattered light generated from an irradiated object due to resonance Raman scattering; and a processor that identifies the irradiated object on the basis of a result detected by the collecting-detecting device 1. A remote substance identification device comprising:a laser device including an oscillator that emits a laser beam of a particular wavelength;a light collecting-detecting device that collects and detects resonance Raman-scattered light from an indoor or outdoor irradiated space; anda processor that identifies an irradiated object present in the irradiated space on the basis of a result detected by the light collecting-detecting device,wherein the remote substance identification device further comprises a wavelength conversion device that converts a wavelength of the laser beam emitted from the laser device into a plurality of different wavelengths and that emits laser beams of the different wavelengths to the irradiated space,the oscillator is an oscillator oscillating the laser beam in a wavelength range longer than an ultraviolet range,the wavelength conversion device includes a first output system that converts the laser beam oscillated from the oscillator into a laser beam of shorter wavelength and outputs the converted laser beam, and a second output system that converts the laser beam output from the first output system into a laser beam ...

Light Measuring Device and Light Measuring Method

To measure a surface state, reflective CARS is suitable in terms of the signal intensity. However, with the reflective CARS, it has been difficult to identify the surface position because the shape information is not acquired. Thus, a reflective CARS microscope is combined with a high-resolution phase sensor. The surface position is identified with the phase sensor, and reflected CARS generated from the surface is detected, so that composition analysis is performed. 1. An optical measuring device comprising:a sample stage that holds a sample;a pump beam generation unit configured to generate a pump beam;a Stokes beam generation unit configured to generate a Stokes beam, the Stokes beam having a wavelength longer than that of the pump beam;a reference beam splitting unit configured to split off a reference beam from the pump beam or the Stokes beam;a combining unit configured to coaxially combine the pump beam with the Stokes beam;an objective lens configured to focus the combined beam of the pump beam and the Stokes beam onto the sample held on the sample stage;a position control unit configured to control a relative position between the objective lens and the sample held on the sample stage;a phase sensor configured to identify a surface position of the sample by causing a reflected beam from the sample that has passed through the objective lens and the reference beam to interfere with each other and detecting an intensity of the reflected beam or a phase of the reflected beam with respect to the reference beam; anda detector configured to detect a reflected CARS beam generated from the sample.2. The optical measuring device according to claim 1 , wherein the phase sensor is configured to detect the surface position of the sample with an accuracy of less than or equal to 3 micrometers in an optical-axis direction.3. The optical measuring device according to claim 1 , wherein a numerical aperture of the objective lens is greater than or equal to 0.4.4. The optical ...

METHODS, SYSTEMS, ARRANGEMENTS AND COMPUTER-ACCESSIBLE MEDIUM FOR PROVIDING MICRO-OPTICAL COHERENCE TOMOGRAPHY PROCEDURES

Exemplary apparatus and method can be provided for obtaining data regarding a plurality of samples. For example, using at least one arrangement, it is possible to receive interferometric information that is based on radiations provided from a reference and the samples that are provided in respective chambers. Alternatively and/or in addition, based on the interferometric information, it is possible to discriminate between agents to identify a particular agent that affects a particular function within at least one of the samples. 126-. (canceled)27. An apparatus for obtaining data regarding a plurality of samples , comprising:an optical coherence tomography (OCT)-based screening system comprising at least one interferometer arrangement which receives interferometric information that is based on radiations provided from a reference interfered with each of the plurality of samples; and obtain data regarding a structure of each of the plurality of samples using the interferometric information,', 'locate one or more features represented in the data, each of the one or more features regarding the structure of a particular sample of the plurality of samples, and', 'calculate a metric that is indicative of a state of the particular sample using the one or more features., 'at least one computer arrangement which is configured to28. The apparatus according to claim 27 , wherein each of the one or more features comprises at least one of a peak in the data claim 27 , an edge claim 27 , a depth of a portion of the sample claim 27 , a size claim 27 , a motion of a portion of the sample claim 27 , and a frequency of motion of a portion of the sample.29. The apparatus according to claim 27 , wherein the at least one interferometer arrangement comprises at least one optics configuration which is configured to focus at least one electromagnetic radiation on the samples claim 27 , andwherein a depth range of the focus of the at least one electromagnetic radiation caused by the at ...

SPECTROSCOPIC METHODS FOR BODY FLUID AGE DETERMINATION

The present invention relates to a method of determining the age of a body fluid stain in a sample. This method involves providing the sample containing a body fluid stain; providing a statistical model for determination of the age of the body fluid stain in the sample; subjecting the sample or an area of the sample containing the stain to a spectroscopic analysis to produce a spectroscopic signature for the sample; and applying the spectroscopic signature for the sample to the statistical model to ascertain the age of the body fluid stain in the sample. A method of establishing a statistical model for determination of the age of a body fluid stain in a sample is also disclosed. 1. A method of determining the age of a body fluid stain in a sample , said method comprising:providing the sample containing a body fluid stain;providing a statistical model for determination of the age of the body fluid stain in the sample;subjecting the sample or an area of the sample containing the stain to a spectroscopic analysis to produce a spectroscopic signature for the sample; andapplying the spectroscopic signature for the sample to the statistical model to ascertain the age of the body fluid stain in the sample.2. The method of claim 1 , wherein the body fluid is selected from the group consisting of blood claim 1 , saliva claim 1 , sweat claim 1 , urine claim 1 , semen claim 1 , and vaginal fluid.35.-. (canceled)6. The method of claim 1 , wherein spectroscopic analysis is Raman spectroscopy.7. The method of claim 6 , wherein the Raman spectroscopy is selected from the group consisting of resonance Raman spectroscopy claim 6 , normal Raman spectroscopy claim 6 , Raman microscopy claim 6 , Raman microspectroscopy claim 6 , NIR Raman spectroscopy claim 6 , surface enhanced Raman spectroscopy (SERS) claim 6 , tip enhanced Raman spectroscopy (TERS) claim 6 , Coherent anti-Stokes Raman scattering (CARS) claim 6 , and Coherent anti-Stokes Raman scattering microscopy.810.-. (canceled) ...

METHODS AND APPARATUSES FOR LABEL-FREE PARTICLE ANALYSIS

An apparatus to provide a label-free or native particle analysis comprises a light generating system producing first light pulses at a first wavelength and second light pulses at a second wavelength; and a flow cell coupled to the light generating system to convey particles for analysis. The light generating system is configured to chirp at least one of the first light pulses and the second light pulses to analyze the particles. 121-. (canceled)22. An apparatus comprising:a light generating system configured to produce a first set of light pulses in a first wavelength region and a second set of light pulses in a second wavelength region, wherein the first and the second wavelength regions do not substantially overlap, and wherein the light generating system is internally configured to positively chirp the first set of light pulses and negatively chirp the second set of light pulses;a first optical system coupled to the light generating system, the first optical system comprising optics configured to combine the first set of chirped light pulses and the second set of chirped light pulses into a set of combined light pulses, wherein each of the combined light pulses comprises a first pulse from the first set of chirped light pulses and a second pulse from the second set of chirped light pulses; anda flow cell coupled to the first optical system and configured to convey, in a flow, particles for analysis, wherein at least one of the particles is exposed to a plurality of the combined light pulses.23. The apparatus of claim 22 , wherein the light generating system comprises temporally dispersive means to positively chirp the first set of light pulses and negatively chirp the second set of light pulses.24. The apparatus of claim 22 , further comprising a synchronization system configured to provide synchronization timing signals to the light generating system claim 22 , wherein the synchronization timing signals are configured to establish a fixed phase relationship ...

OPTICAL ANALYSIS DEVICE AND BIOMOLECULAR ANALYSIS DEVICE

In a multi-color CARS microscope, it has been difficult to accurately bring optical axes of pump light and Stokes light into correspondence and to stably acquire a spectral signal. 1. An optical analysis device comprising:a short-pulse laser light source;an optical waveguide that generates super continuum light by photoexcitation;a first collecting optical system that collects and introduces a light beam from the light source to the optical waveguide;a filter that passes light emitted from the optical waveguide, and eliminates a wavelength component shorter than a wavelength of the light beam from the light source;a second collecting optical system that collects the light beam passed through the filter to a sample; anda spectroscope that detects CARS light generated from the sample.2. The optical analysis device according to claim 1 , wherein the optical waveguide is photonic crystal fiber.3. The optical analysis device according to claim 2 , wherein the length of the photonic crystal fiber is within 1 m.4. The optical analysis device according to claim 1 , further comprising control means for controlling a relative position between the second collecting optical system and means for holding the sample.5. The optical analysis device according to claim 4 , wherein the control means is a mirror that scans an incident angle of the second collecting optical system.6. An optical analysis device comprising:a short-pulse laser light source that has output with a pulsewidth within 500 femtoseconds;an optical waveguide that generates super continuum light by photoexcitation;a collecting optical system that collects and introduces a light beam from the light source to the optical waveguide;a filter that passes light emitted from the optical waveguide, and passes a spectral component as a part of spectral components of the light beam from the light source and a wavelength component longer than a spectrum of the light beam from the light source;a collecting optical system that ...

A fluid analytical device

A fluid analytical device comprising: a disc (1) rotatable around an axis (2), the disc (1) comprising: a first layer, the first layer comprising: a disc; and at least one microfluidic channel (6) in the disc partially extending from the disc axis (2) to the disc edge; a second layer, the second layer comprising: a disc of substantially the same diameter as the disc of the first layer; and at least one through input port (3) and one through measurement port (4) pair, the input port (3) located near the disc axis (2) and the measurement port (4) located distal from the disc axis (2); wherein when assembled each of the at least one input port (3) and measurement port (4) pair are aligned with one of the at least one microfluidic channels (6); and a disc spinning mechanism (12); a controller (13) for controlling the disc spinning mechanism (12); and a microscope (11) for analysing the fluid through the measurement ports (4) in the rotatable disc (1).

MEDICAL SENSOR HAVING A NANOSCALE TAPERED WAVEGUIDE FOR SPECTROSCOPY-BASED ANALYSIS OF FLUID

A medical sensor is described. In an example, the medical sensor includes a nanoscale tapered waveguide attached to a substrate. The nanoscale tapered waveguide includes a nanoscale channel that receives fluid and an excitation light and that outputs a response light. The excitation light propagates through the fluid. A receiving channel of the nanoscale channel is configured as a waveguide that receives and guides the excitation to a linearly tapered channel of the nanoscale channel. The linearly tapered channel has three dimensional linear tapering that focuses the excitation light guided from the receiving channel into an optical response channel of the nanoscale channel. In turn, the optical response channel is configured as a waveguide that outputs a response light in response to the excitation light focused from the linearly tapered channel. The response light corresponds to a response of an analyte of the fluid present in the optical response channel. 1. A medical sensor , comprising:a substrate; and a receiving channel having a height within a range of 250 nm to 750 nm and a width within a range of 150 nm to 250 nm, the receiving channel formed by at least a first portion of a first rail and a first portion of a second rail, the first portions substantially parallel to each other, the receiving channel configured to receive fluid and guide an excitation light;', 'a linearly tapered channel configured to receive the fluid and focus the excitation light guided from the receiving channel, the linearly tapered channel formed by at least a second portion of the first rail and a second portion of the second rail, the second portions linearly tapered relative to each other along two axes; and', 'an optical response channel configured to receive the fluid and to output a response light to the excitation light focused from the linearly tapered channels, the optical response channel formed by at least a third portion of the first rail and a third portion of the second ...

Optical Measurement Apparatus and Optical Measurement Method

In multicolor Coherent anti-Stokes Raman Scattering, a fingerprint region is a region densely filled with signals, and information about an intensity or spatial distribution of the signals is important for cell analysis. However, when laser power was increased due to the low signal intensity, there was a problem that cells were damaged. Thus, the present invention was configured such that the number of pulses of laser light emitted from a short pulse laser light source was changed by a pulse modulator. Accordingly, damage to the cells can be suppressed without significant reduction in signal intensity. 1. An optical measurement apparatus , comprising:a short pulse laser light source;a modulator that is arranged in a light path between the short pulse laser light source and a sample and modulates power of predetermined pulses out of pulses of laser light so as to reduce the power, the laser light being generated from the short pulse light source;a branching unit that branches emission light from the short pulse laser light source into a first light flux and a second light flux;an optical fiber that generates super-continuum light from the first light flux;a multiplex unit that multiplexes Stokes light and pump light, the Stokes light being a long wavelength component out of the super-continuum light, the pump light being the second light flux;a condensing optical system that condenses light multiplexed by the multiplex unit on a sample; anda spectroscope that detects light generated from the sample.2. The optical measurement apparatus according to claim 1 , wherein the modulator modulates the predetermined pulses so as not to irradiate.3. The optical measurement apparatus according to claim 1 , wherein the modulator reduces number of pulses of the laser light to ½ or less.4. The optical measurement apparatus according to claim 1 , wherein the modulator reduces power of predetermined pulses of the laser light to ½ or less.5. The optical measurement apparatus according ...

Measuring apparatus and measuring method

Provided is a measuring apparatus including: a light source unit to emit pulsed laser light used for pump light and Stokes light that excite a molecular vibration of a sample; a Stokes light generating unit to modulate an intensity of the pulsed laser light and to generate Stokes light using the pulsed laser light having the modulated intensity; a time delaying unit to delay the pump light using the pulsed laser light or the Stokes light; a detecting unit to detect, by lock-in detection, light transmitted through the sample irradiated with the pump light and the Stokes light having a controlled time delay amount, or reflected light from the sample; and an arithmetic processing device to perform arithmetic processing on the basis of anti-Stokes light detected by the lock-in detection while controlling the intensity modulation and the time delay amount.

OPTICAL DETECTION DEVICE, OPTICAL DETECTION METHOD, AND PROGRAM

A phase sensitive detection mechanism that uses electrical processing is realized, and an optical detection device, an optical detection method, and a program that are capable of detecting faint light at high speed and with high sensitivity are provided by a simple configuration. 26-. (canceled)7. The optical detection device of claim 1 , wherein:the light source section is a light source that employs an ultrashort pulse laser; andthe bandwidth of the frequency spectrum of the second pulsed light is narrower than the bandwidth of the frequency spectrum of the third pulsed light.8. The optical detection device of claim 1 , wherein the phase modulation section is a modulator based on electro-optical effects claim 1 , or a light path length adjustment section that changes a light path length for incident light and emits the incident light.9. The optical detection device of claim 1 , wherein the filter section and the multiplexing section are configured by a single bandpass filter that transmits the second pulsed light and the second pulsed light phase modulated by the phase modulation section claim 1 , and that reflects the third pulsed light.10. The optical detection device of claim 1 , wherein the frequency spectrum extracted by the extraction section is a frequency spectrum of coherent anti-Stokes Raman scattered light. The present invention relates to an optical detection device, an optical detection method, and a program.The present invention relates to an optical detection device, an optical detection method, and a program, and in particular, relates to an optical detection device, an optical detection method, and a program employing Raman spectroscopy technology in the analysis of substances. Namely, the present invention is related to an optical detection device in which two or more beams of pulsed laser light are radiated onto a sample, and substances in the sample are analyzed by observing Raman scattered light that is emitted from the sample as a result.The ...

INNOVATIVE NANOPORE SEQUENCING TECHNOLOGY

Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques. 1. A method for forming a device for the manipulation of targeted long chain molecules comprising:providing a nanochannel having a common porous roof comprising a plurality of tortuous nanopores; andsealing some of the nanopores in the porous roof via conformal atomic layer deposition (ALD).2. The method of further comprising forming a layer over the roof via chemical vapor deposition (CVD).3. The method of wherein the size of at least one of the unsealed pores enables translocation of single long chain molecules one at a time.4. The method of wherein the density of the unsealed nanopores enables optical resolution of individual nanopores.5. The method of further comprising depositing a field enhancement structure on the roof of the nanochannel.6. The method of wherein the field enhancement structure is self-aligned to the nanopores.7. The method of further comprising forming an additional optically transparent layer spaced away from the field enhancement structure.8. The method of wherein the field enhancement structure is a metal insulator metal (MIM) structure.9. The method of wherein the MIM structure is deposited by a combination of anisotropic and isotropic ...

BIOPSY DEVICE FOR COHERENT RAMAN IMAGING

Devices and systems for analyzing biological samples are provided. Devices include a hollow body extending from a first end to a second end. The body defines a sample collecting portion. A first opening at the first end of the body is operable to receive a source of negative pressure and a second opening at the second end of the body is operable to receive a biological sample. The body also includes an optically transparent region disposed in a region corresponding to the sample collecting portion, the optically transparent region being configured to transmit electromagnetic radiation therethrough from an imaging device capable of imaging the biological sample when disposed in the sample collecting portion. 1. A device comprising:a body extending from a first end to an opposing second end and defining a collection channel extending from a first opening at the first end to a second opening at the second end, the first opening being in communication with a source of negative pressure, and the collection channel being configured to receive a biological sample from a subject;a scanner integrated into the body, the scanner being configured to emit light across the collection channel and through the biological sample; anda detector integrated into the body, the detector being configured to receive lighted emitted from the scanner and to capture an image of the biological sample.2. The device according to claim 1 , further comprising:a lens integrated into the body, the lens being configured to focus light emitted from the scanner to the detector.3. The device according to claim 1 , wherein the collection channel is straight.4. The device according to claim 1 , wherein the collection channel has an angled turn.5. The device according to claim 1 , wherein the source of negative pressure is a hospital's vacuum system.6. The device according to claim 1 , wherein the imaging is transmission imaging.7. The device according to claim 1 , wherein the imaging is stimulated Raman ...

METHODS AND APPARATUSES FOR LABEL-FREE PARTICLE ANALYSIS

An apparatus to provide a label-free or native particle analysis comprises a light generating system producing first light pulses at a first wavelength and second light pulses at a second wavelength; and a flow cell coupled to the light generating system to convey particles for analysis. The light generating system is configured to chirp at least one of the first light pulses and the second light pulses to analyze the particles. 121-. (canceled)22. An apparatus comprising:a light generating system configured to produce a first set of light pulses in a first wavelength region and a second set of light pulses in a second wavelength region, wherein the first and the second wavelength regions do not substantially overlap, and wherein the light generating system is internally configured to chirp at least one of: the first set of light pulses and the second set of light pulses;a first optical system coupled to the light generating system, the first optical system comprising optics configured to combine the first set of light pulses and the second set of light pulses into a set of combined chirped light pulses, wherein each of the combined chirped light pulses comprises a first pulse from the first set of light pulses and a second pulse from the second set of light pulses; anda flow cell coupled to the first optical system and configured to convey, in a flow, particles for analysis, wherein at least one of the particles is exposed to a plurality of the combined chirped light pulses.23. The apparatus of claim 22 , wherein the light generating system comprises temporally dispersive means to chirp the at least one of: the first set of light pulses and the second set of light pulses.24. The apparatus of claim 22 , further comprising a synchronization system configured to provide synchronization timing signals to the light generating system claim 22 , wherein the synchronization timing signals are configured to establish a fixed phase relationship between the first set of light ...

Optical analyzer

RAMAN-ACTIVE NANOPARTICLE FOR SURFACE-ENHANCED RAMAN SCATTERING AND METHOD OF PRODUCING THE SAME

Provided is a Raman-active nanoparticle including: a spherical plasmonic metal core; a plasmonic metal shell having surface irregularities; and a self-assembled monolayer which binds to each of the core and the shell, is positioned between the core and the shell, and includes a Raman reporter satisfying the following Chemical Formula 1: 1. A Raman-active nanoparticle comprising:a spherical plasmonic metal core;a plasmonic metal shell having surface irregularities; and {'br': None, 'sub': '2', 'NO—Ar—SH\u2003\u2003[Chemical Formula 1]'}, 'a self-assembled monolayer which binds to each of the core and the shell, is positioned between the core and the shell, and includes a Raman reporter satisfying the following Chemical Formula 1wherein Ar is a (C6-C12) arylene group.3. The Raman-active nanoparticle of claim 2 , whereinthe plasmonic metal shell includes plasmonic metal fine particles having an average size of 0.3D to 1D, based on a diameter (D) of the metal core, and has surface irregularities due to the plasmonic metal fine particles.4. The Raman-active nanoparticle of claim 3 , whereinin the plasmonic metal shell, an inner shape of the shell in contact with the self-assembled monolayer is spherical.5. The Raman-active nanoparticle of claim 3 , whereinthe plasmonic metal core has an average diameter of 20 to 100 nm.6. The Raman-active nanoparticle of claim 3 , whereinthe self-assembled monolayer has a thickness of 0.5 to 2.0 nm.7. The Raman-active nanoparticle of claim 1 , whereinthe plasmonic metal core and the plasmonic metal shell are independently of each other one or more metals selected from gold, silver, platinum, palladium, nickel, aluminum, and copper.8. The Raman-active nanoparticle of claim 7 , whereinthe plasmonic metal core and the plasmonic metal shell are the same metal.9. The Raman-active nanoparticle of claim 1 , further comprising:a receptor which is fixed to the plasmonic metal shell and binds to the analyte.10. The Raman-active nanoparticle of ...

Intra-nanogapped core-shell nanoparticle and preparation method thereof

본 발명은 나노갭에 의한 플라즈몬 커플링(plasomonic coupling)에 의한 매우 높은 신호 증폭의 효과 및 높은 재현성을 가지고 있어 라만 분석에 유용하게 사용될 수 있는, 코어(core) 및 상기 코어를 둘러싼 쉘(shell)을 포함하고, 상기 코어와 쉘 사이에 나노갭이 형성된 나노입자 및 이의 제조방법을 제공한다. 또한, 상기 나노입자를 이용한 분석물을 검출하는 방법 및 상기 나노입자를 포함하는 분석물 검출용 키트를 제공하기 위한 것이다. The present invention has a core and a shell surrounding the core, having a very high signal amplification effect and high reproducibility by plasmon coupling by nanogap, which can be useful for Raman analysis. It includes, and provides a nanoparticle and a method for producing a nanogap formed between the core and the shell. The present invention also provides a method for detecting an analyte using the nanoparticles and a kit for detecting an analyte including the nanoparticles.

Coherent anti-Stokes Raman scattering microscope imaging system

Method and Apparatus for Selective Suppression of Coherent Anti-Stokes Raman Scattering Signal

본 발명의 일 실시예에 따른 선택적 가간섭성 반스톡스 라만산란 신호 억제 방법은, 3빔 경쟁 유도라만 산란(SRS; Stimulated Raman Scattering)을 이용한 선택적 가간섭성 반스톡스 라만산란(CARS; Coherent anti-Stokes Raman scattering) 신호 억제 방법에 있어서, 3개의 빔이 입사되는 단계; 입사된 상기 3개의 빔에 의해 제1 CARS 신호 및 제2 CARS 신호가 발생되는 단계; 상기 제1 CARS 신호 및 제2 CARS 신호에 의해 제1 SRS 과정 및 제2 SRS 과정이 유발되는 단계; 및 상기 제1 SRS 과정 및 제2 SRS 과정의 경쟁에 의해 상기 제1 CARS 신호를 억제하는 단계를 포함할 수 있다. Selective coherent anti-Stokes Raman scattering signal suppression method according to an embodiment of the present invention, selective coherent anti-Stokes Raman scattering (CARS; Coherent anti-Raman scattering) using 3-beam competition induced Raman scattering (SRS) In the Stokes Raman scattering) signal suppression method, the method comprising: incident three beams; generating a first CARS signal and a second CARS signal by the three incident beams; inducing a first SRS process and a second SRS process by the first CARS signal and the second CARS signal; and suppressing the first CARS signal by competition between the first SRS process and the second SRS process.

Apparatus for processing a biological and/or chemical sample

The present invention is directed to an apparatus for processing at least one biological and/or chemical sample. The apparatus comprises a substrate, temperature control modules and a rotatable platform carrying a magnetic field generator and an optical unit. The present invention is further directed to a system including the apparatus and magnetically attractable matter as well as method which can be carried out using the apparatus of the present invention.

Image acquisition device and method using coherent anti-stokes Raman scattering

가간섭성 반스토크스 라만 산란을 이용한 영상 획득 장치 및 방법이 개시된다. 본 발명에 따른 가간섭성 반스토크스 라만 산란을 이용한 영상 획득 장치는, 반스토크스 주파수를 가지는 반스토크스 광을 발생시키기 위해 펌프 광 및 스토크스 광을 시료에 조사하기 위한 펌프 광원 및 스토크스 광원, 참조광을 발생시키기 위한 참조 광원 및 상기 반스토크스 주파수 근처에서의 상기 시료의 굴절률 변화로 인한 상기 참조광의 위상 변화를 이용하여 상기 시료의 영상을 획득하는 영상 획득 수단을 포함하는 것을 특징으로 한다. 이러한 본 발명에 의하면 비공명 배경 신호 현상의 영향을 받지 않고, 약한 신호에서도 잡음에 강하며, 우수한 감도 및 분해능을 가지는 가간섭성 반스토크스 라만 산란을 이용한 영상 획득 장치를 제공할 수 있다. Disclosed are an apparatus and method for image acquisition using coherent anti-stokes Raman scattering. An image acquisition apparatus using coherent anti-stokes Raman scattering according to the present invention, the pump light source and the stokes for irradiating the pump light and stokes light to the sample to generate anti-stokes light having the anti-stokes frequency And an image acquiring means for acquiring an image of the sample by using a light source, a reference light source for generating reference light, and a phase change of the reference light due to a change in refractive index of the sample near the anti-stokes frequency. . According to the present invention, it is possible to provide an image acquisition apparatus using coherent anti-stokes Raman scattering, which is not influenced by a non-resonant background signal phenomenon, is resistant to noise even in a weak signal, and has excellent sensitivity and resolution.

Nonlinear vibrational microscopy

The present invention is a method and apparatus for microscopic vibrational imaging using coherent Anti-Stokes Raman Scattering or Sum Frequency Generation. Microscopic imaging with a vibrational spectroscopic contrast is achieved by generating signals in a nonlinear optical process and spatially resolved detection of the signals. The spatial resolution is attained by minimizing the spot size of the optical interrogation beams on the sample. Minimizing the spot size relies upon a. directing at least two substantially co-axial laser beams (interrogation beams) through a microscope objective providing a focal spot on the sample; b. collecting a signal beam together with a residual beam from the at least two co-axial laser beams after passing through the sample; c. removing the residual beam; and d. detecting the signal beam thereby creating said pixel. The method has significantly higher spatial resolution then IR microscopy and higher sensitivity than spontaneous Raman microscopy with much lower average excitation powers. CARS and SFG microscopy does not rely on the presence of fluorophores, but retains the resolution and three-dimensional sectioning capability of confocal and two-photon fluorescence microscopy. Complementary to these techniques, CARS and SFG microscopy provides a contrast mechanism based on vibrational spectroscopy. This vibrational contrast mechanism, combined with an unprecedented high sensitivity at a tolerable laser power level, provides a new approach for microscopic investigations of chemical and biological samples.

Surface-enhanced Raman spectroscopy immunoassay

A method, composition, device, apparatus, and kit for the determination of the presence or amount of an analyte by monitoring an analyte-mediated ligand binding event in a test mixture which contains the analyte to be assayed, a specific binding member, a Raman-active label, and a particulate having a surface capable of inducing a surface-enhanced Raman light scattering. The test mixture is illuminated with a radiation sufficient to cause the Raman-active label in the test mixture to emit a detectable Raman spectrum. The differences in the detected surface-enhanced Raman scattering spectra are dependent upon the amount of the analyte present in the test mixture. Thus, by monitoring these differences, the presence or amount of the analyte are determined.

Autofocus mechanism for spectroscopic system

本发明提供一种用于光谱系统(400)的自动聚焦机构，所述光谱系统适用于确定所关心容积的性质。该所关心容积具有随时间变化的光学性质。本发明提供一种测量装置，该装置适用于测量所关心容积的光学性质的波动，从而确定所关心容积的位置(428)。该光谱系统还适用于将激发光束(418)聚焦到确定的所关心容积中，并用于收集从所关心容积发出的返回辐射(420)以用于光谱分析。优选地，激发光束(428)的非弹性散射辐射与用于光谱分析的弹性散射辐射分开。再利用激发光束的弹性散射辐射来测量所关心容积的光学性质的波动。利用控制回路，使得波动的振幅和/或强度能够最大化，该最大值固有地规定了所关心容积的位置，例如毛细血管(450)的中心。

Innovative nanopore sequencing technology

用于长阅读、无标记、光学纳米孔长链分子测序的方法和设备。总体来说，本公开描述新颖测序技术，其基于并入纳米通道以使用宽间隔(>波长)、～1‑nm孔径“曲折”纳米孔来递送单一长链分子，所述纳米孔充分地减缓易位以便使用光学技术来提供大规模并行、单一碱基解析。使用简单胶状纳米颗粒的新颖、定向自组装纳米制造方案用于在纳米通道顶上形成纳米孔阵列，从而使长链分子展开。在纳米颗粒阵列的表面，工程化等离子/极化声子结构中的强烈局部电磁场允许使用光学技术来进行单一碱基解析。

Use of vibrational spectroscopy for microfluidic liquid measurement

This disclosure concerns a cytometry system including a handling system that enables presentation of single cells to at least one laser source. The laser source is configured to deliver light to a cell within the cells in order to induce bond vibrations in the cellular DNA. The system further includes a detection facility that detects the signature of the bond vibrations, wherein the bond vibration signature is used to determine the folding or packing of the DNA.

Optical lattice microscopy

A periodic interference pattern (1201, 1314) of coherent waves is created in two or three dimensions, D, by generating at least D+l waves (700, 701, 702), each wave having substantially the same wavelength, λ, and traveling in substantially in a unique direction, kn, and directing the waves such that at least a portion of each wave intersects in a common excitation region to create the interference pattern. The directions of the waves, kn, are selected such that the interference pattern within the excitation region forms a Bravais lattice (1201, 1314) that has a symmetry and that has a shape to a primitive cell of the lattice, which is defined by the D-dimensional region closest to any chosen lattice point (1307, 1308, 1309, 7407). The directions of the waves, kn, also are selected such that a periodicity of the lattice relative to the wavelength, λ, is larger that the smallest periodicity Bravais lattice having the same symmetry and identically defined primitive cell shape that can be created at the wavelength, λ. The direction of the waves, kn, do not all lie on a D-dimensional set of mutually orthogonal axes.

Vessel imaging system

A system and a method for acquiring an image of a particle flowing in a vessel, the system comprising a light source for generating an illuminating light, an imaging probe for laterally statically illuminating at least a portion of said vessel with the illuminating light, a detection unit for detecting emitted light from an illuminated portion of said particle, and a processor unit for reproducing an image of the illuminated portion of said particle from the emitted light.

Vessel imaging system and method

A system and a method for acquiring an image of a particle flowing in a vessel, the system comprising a light source for generating an illuminating light, an imaging probe for laterally statically illuminating at least a portion of said vessel with the illuminating light, a detection unit for detecting emitted light from an illuminated portion of said particle, and a processor unit for reproducing an image of the illuminated portion of said particle from the emitted light.

Wafer inspection apparatus and system including same

본 발명의 기술적 사상에 따른 예시적인 실시예들에 따르면, 웨이퍼 검사 장치가 제공된다. 상기 장치는, 제1 및 제2 입력 빔들의 광 경로 상에 있는 대물 렌즈; 및 상기 제1 및 제2 입력 빔들에 기초한 비선형 광학 현상에 따른 산란 광에 기초하여 웨이퍼의 이미지를 생성하는 이미지 센서를 포함하되, 상기 대물 렌즈를 통과한 상기 제1 입력 빔은 상기 웨이퍼의 상면의 법선에 대해 제1 입사 각도로 상기 웨이퍼에 비스듬하게 입사하고, 상기 대물 렌즈를 통과한 상기 제2 입력 빔은 상기 웨이퍼의 상면의 법선에 대해 비스듬한 제2 입사 각도로 상기 웨이퍼에 입사하되, 및 상기 제1 및 제2 입사 각도는 서로 다르다.

Methods, systems, arrangements and computer-accessible medium for providing micro-optical coherence tomography procedures

Exemplary apparatus and method can be provided for obtaining data regarding a plurality of samples. For example, using at least one arrangement, it is possible to receive interferometric information that is based on radiations provided from a reference and the samples that are provided in respective chambers. Alternatively and/or in addition, based on the interferometric information, it is possible to discriminate between agents to identify a particular agent that affects a particular function within at least one of the samples.

Nonlinear interferometric vibrational imaging

A method of examining a sample, which includes: exposing a reference to a first set of electromagnetic radiation, to form a second set of electromagnetic radiation scattered from the reference; exposing a sample to a third set of electromagnetic radiation to form a fourth set of electromagnetic radiation scattered from the sample; and interfering the second set of electromagnetic radiation and the fourth set of electromagnetic radiation. The first set and the third set of electromagnetic radiation are generated from a source; at least a portion of the second set of electromagnetic radiation is of a frequency different from that of the first set of electromagnetic radiation; and at least a portion of the fourth set of electromagnetic radiation is of a frequency different from that of the third set of electromagnetic radiation.

Distinguishing non-resonant four-wave-mixing noise in coherent stokes and anti-stokes Raman scattering

A method of examining a sample comprises exposing the sample to a pump pulse of electromagnetic radiation for a first period of time, exposing the sample to a stimulant pulse of electromagnetic radiation for a second period of time which overlaps in time with at least a portion of the first exposing, to produce a signal pulse of electromagnetic radiation for a third period of time, and interfering the signal pulse with a reference pulse of electromagnetic radiation, to determine which portions of the signal pulse were produced during the exposing of the sample to the stimulant pulse. The first and third periods of time are each greater than the second period of time.

Coherent anti-stokes raman spectroscopy

METHOD FOR THE DETECTION OF A RESONANT NONLINEAR OPTICAL SIGNAL AND DEVICE FOR THE IMPLEMENTATION OF SAID METHOD

L'invention concerne une méthode et un dispositif pour détecter un signal optique non linéaire résonant induit dans un échantillon (805) comprenant un milieu résonant (61) et un milieu non résonant formant une interface, le dispositif comprenant : - une source d'émission (801) d'au moins un premier faisceau lumineux d'excitation du milieu résonant, dit faisceau pompe, à une première pulsation ωp donnée, ledit faisceau pompe étant incident sur l'échantillon selon un axe optique, et interceptant l'échantillon à une position donnée d'une interface transverse entre le milieu résonant et non résonant, - un premier module optique (803) de détection du signal optique non linéaire résultant de l'interaction du ou desdits faisceaux avec l'échantillon, - des moyens de réflexion (813) du ou desdits faisceaux d'excitation, le ou les faisceaux d'excitation ainsi réfléchis interceptant ladite interface transverse sensiblement à la même position que le ou les faisceaux d'excitation incidents, - un deuxième module optique (806) de détection du signal optique non linéaire résultant de l'interaction du ou desdits faisceaux d'excitation réfléchis avec l'échantillon, - un module de traitement (830) des signaux optiques détectés par lesdits premier et second modules de détection, comprenant le calcul d'une différence des signaux détectés, la différence étant caractéristique d'une résonance vibrationnelle ou électronique du milieu résonant. The invention relates to a method and a device for detecting a resonant nonlinear optical signal induced in a sample (805) comprising a resonant medium (61) and a non-resonant medium forming an interface, the device comprising: a source of emission (801) of at least a first excitation light beam of the resonant medium, said pump beam, at a first given pulsation ωp, said pump beam being incident on the sample along an optical axis, and intercepting the sample at a given position of a ...

Optical measuring device and optical measuring method

Bei Multiplex-CARS ist ein Fingerabdruckbereich ein Bereich, in dem Signale dicht konzentriert sind. Informationen über die Intensitäten und die räumliche Verteilung dieser Signale sind bei der Zellenanalyse wichtig. Es bestand jedoch insofern ein Problem, dass die Signalintensitäten niedrig sind. Folglich werden Mechanismen 702 und 703 zum Einstellen des Leistungsverzweigungsverhältnisses zwischen Licht, das in eine photonische Kristallfaser 705 eintritt, und Pumplicht; Mechanismen 710 und 711 zum Einstellen des Divergenz/Konvergenz-Zustandes des Pumplichts; und Mechanismen 706 und 2101 zum Einstellen des Divergenz/Konvergenz-Zustandes von Stokes-Licht geschaffen, um eine Einstellung zum Betonen eines gewünschten Wellenlängenbandes zu ermöglichen. In multiplex CARS, a fingerprint area is an area in which signals are tightly concentrated. Information about the intensities and spatial distribution of these signals is important in cell analysis. However, there has been a problem in that the signal intensities are low. Thus, mechanisms 702 and 703 for adjusting the power-split ratio between light entering a photonic crystal fiber 705 and pump light; Mechanisms 710 and 711 for adjusting the divergence / convergence state of the pump light; and providing mechanisms 706 and 2101 for adjusting the divergence / convergence state of Stokes light to enable adjustment to emphasize a desired wavelength band.

Patent FR3036430A1

La présente description concerne des systèmes et des procédés d'analyse de fluides. Le procédé d'analyse d'un échantillon de produit chimique dans un puits de forage (4), contenu dans un dispositif d'analyse (12), peut comprendre l'émission d'une lumière cohérente à partir d'un module à peigne de fréquences (10), la direction de la lumière cohérente à travers une ligne de type fibre optique (8) jusqu'au dispositif d'analyse (12), l'irradiation de l'échantillon de produit chimique avec la lumière cohérente, captation de la lumière obtenue par irradiation de l'échantillon de produit chimique et la production d'un spectre résultant de la lumière captée à partir de l'échantillon de produit chimique. L'invention concerne aussi un système à peigne de fréquences (2) pour analyser un échantillon de produit chimique, qui peut comprendre un module à peigne de fréquences (10) conçu pour émettre une lumière cohérente et une ligne de type fibre optique (8) qui s'étend dans un puits de forage (4) jusqu'à un dispositif d'analyse (12). Le dispositif d'analyse (12) peut être conçu pour contenir l'échantillon de produit chimique pour une irradiation par la lumière cohérente. Le système à peigne de fréquences (2) peut en outre comprendre un récepteur (14) et un système de traitement d'informations (6). The present disclosure relates to systems and methods for fluid analysis. The method of analyzing a chemical sample in a wellbore (4), contained in an analysis device (12), may include the emission of coherent light from a comb module of frequencies (10), the direction of coherent light through an optical fiber-type line (8) to the analysis device (12), the irradiation of the chemical sample with the coherent light, capturing light obtained by irradiating the chemical sample and producing a spectrum resulting from light captured from the chemical sample. The invention also relates to a frequency comb ...

Multi-modal optical imaging system for tissue analysis

A modular system for organic sample analysis is disclosed which includes a sample stage including a support platform and a motorized positioning mechanism mounted on the support platform, and a sample holder mounted on the motorized positioning mechanism upon which a sample is placed. A probe support rack is mounted on the support platform and two or more bio-imaging probes mounted on the probe support and arranged in a predefined geometry with respect to each other, and at least one bio-imaging probe hays a field of view independent of all other bio-imaging probes. The system includes a computer controller connected to the motorized positioning mechanism and the two or more bio-imaging probes. The computer is programmed for controlling motorized positioning mechanism to move the sample holder having the sample located thereon to positions in the field of view of each bio-imaging probe where the sample can be analyzed individually by each of the bio-imaging probes. The computer includes a storage medium for storing an imaging data from each bio-imaging probe. The computer is programmed for spatially correlating imaging data of the selected volume of interest with the imaging data obtained from at least one other bio-imaging probe and storing the spatially correlated imaging data in the computer storage medium.

Spectroscopic analysis system and method

Spectroscopic analysis systems and methods for analyzing samples are disclosed. An analysis system may contain an electromagnetic radiation source to provide radiation, a spectroscopic analysis chamber to perform a coherent Raman spectroscopy (e.g., stimulated Raman or coherent anti-Stokes Raman spectroscopy), and a radiation detector to detect radiation based on the spectroscopy. The chamber may have a resonant cavity to contain a sample for analysis, at least one window to the cavity to transmit the first radiation into the cavity and to transmit a second radiation out, a plurality of reflectors affixed to a housing of the cavity to reflect radiation of a predetermined frequency, the plurality of reflectors separated by a distance that is sufficient to resonate the radiation. The spectroscopic analysis system may be coupled with a nucleic acid sequencing system to receive a single nucleic acid derivative in solution and identify the derivative to sequence the nucleic acid.

Systems and methods for imaging and processing tissue

In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue, capturing the sectioned tissue, and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, and capturing each sectioned tissue, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest. Further, captured tissue sections can be processed, re-imaged, and indexed to their original location in the three dimensional image.

Determination device, determination program, determination method, cell sheet manufacturing device, and cell sheet manufacturing method

Nucleic acid sequencing by Raman monitoring of uptake of precursors during molecular replication

The methods, compositions and apparatus disclosed herein are of use for nucleic acid sequence determination. The methods involve isolation of one or more nucleic acid template molecules and polymerization of a nascent complementary strand of nucleic acid, using a DNA or RNA polymerase or similar synthetic reagent. As the nascent strand is extended one nucleotide at a time, the disappearance of nucleotide precursors from solution is monitored by Raman spectroscopy or FRET. The nucleic acid sequence of the nascent strand, and the complementary sequence of the template strand, may be determined by tracking the order of incorporation of nucleotide precursors during the polymerization reaction. Certain embodiments concern apparatus comprising a reaction chamber and detection unit, of use in practicing the claimed methods. The methods, compositions and apparatus are of use in sequencing very long nucleic acid templates in a single sequencing reaction.

Measuring device and measuring method

計測対象分子の変更に伴う干渉光学系の調整を不要とすることが可能な計測装置を提供すること。計測装置１は、ポンプ光ＰＰとストークス光ＳＴとシグナル光ＳＧを発生する光源部１０と、ポンプ光ＰＰ及びストークス光ＳＴを被検体Ｓへ照射する光照射部２０と、シグナル光ＳＧの波長を変調する光変調部３０と、波長変調されたシグナル光ＳＧと被検体Ｓからのアンチストークス光ＡＳとの干渉光を検出する第１光検出部５０と、シグナル光ＳＧと波長変調されたシグナル光ＳＧとの干渉光を検出する第２光検出部５２と、第１光検出部５０からの信号から第２光検出部５２からの信号との位相差が９０°の成分を検出する信号検出部６０を含み、光源部１０の光パラメトリック発振器１２は、光路に対して互いに逆方向に同一角度傾くように回転する２つの非線形光学結晶を有する。 To provide a measuring apparatus that can eliminate the need for adjustment of an interference optical system in accordance with a change of a molecule to be measured. The measuring apparatus 1 includes a light source unit 10 that generates pump light PP, Stokes light ST, and signal light SG, a light irradiation unit 20 that irradiates the subject S with pump light PP and Stokes light ST, and a wavelength of the signal light SG. A light modulating unit 30 for modulating, a first light detecting unit 50 for detecting interference light between the wavelength-modulated signal light SG and the anti-Stokes light AS from the subject S, and the signal light SG and the wavelength-modulated signal light A second light detection unit 52 that detects interference light with SG, and a signal detection unit that detects a component whose phase difference between the signal from the first light detection unit 50 and the signal from the second light detection unit 52 is 90 °. The optical parametric oscillator 12 of the light source unit 10 includes two nonlinear optical crystals that rotate so as to be inclined at the same angle in opposite directions with respect to the optical path.

Mitigation of inaccuracies related to grating asymmetries in scatterometry measurements

회절 기반 오버레이 측정들에서의 격자 비대칭성들의 영향들을 완화시키는, 산란측정 오버레이 타깃들은 물론 타깃 설계 및 측정 방법들이 제공된다. 타깃들은 - 격자 비대칭성들로부터 결과하는 부정확성들을 격리시키고 제거하기 위해 - 거친 분해능의 피치 격자들을 대체하는 서브 분해능의 구조체들을 갖는 추가의 셀들을 포함하고 그리고/또는 거친 피치 주기성을 갖는 교호하는 서브 분해능의 구조체들을 포함한다. 측정 방법들은, 설계된 타깃 구조체들과 관련하여, 상이한 측정 방향들에서의 격자 비대칭성 영향들을 격리시키기 위해 직교 편광된 조명을 이용한다.

Cell observation method, cell observation device, cell observation program, cell-sheet manufacturing method, and cell-sheet manufacturing device

A cell observation method that has the following steps: a detection step in which emitted light emitted by a subject of observation upon being illuminated with illuminating light is detected; and a determination step in which, on the basis of said emitted light, it is determined whether cells in the subject of observation are dead or alive. Also, a cell observation method that has the following steps: a detection step in which emitted light emitted by a subject of observation upon being illuminated with illuminating light is detected; and a detection step in which, on the basis of said emitted light, the number of living cells in the subject of observation is counted. Furthermore, a cell observation method that has the following steps: a detection step in which emitted light emitted by a subject of observation upon being illuminated with illuminating light is detected; and a detection step in which, on the basis of said emitted light, the state of fat and/or protein in the subject of observation is detected.

Optical analysis device

抑制数据量而高速地取得试样的CARS光谱等的光谱数据。在聚光于试样而照射的照射光扫描中，维持对从试样产生的光进行分光的分光器的检测部的曝光状态，取得通过将在试样内的多个位置产生的光谱进行累积而得到的光谱数据。

Identification of body fluids using raman spectroscopy

The present invention relates to a method of identifying types of body fluids in a sample. This method involves providing a sample potentially containing one or more types of body fluids. The sample is subjected to Raman spectroscopy to produce a Raman spectroscopic signature for the sample. The Raman spectroscopy signature is identified to ascertain the types of body fluids in the sample. A method of establishing a reference Raman spectroscopic signature for specific types of body fluids is also disclosed as is a library of such reference signatures is also disclosed.

Spectroscopic analysis system and method

Spectroscopic analysis systems and methods for analyzing samples are disclosed. An analysis system may contain an electromagnetic radiation source to provide radiation, a spectroscopic analysis chamber to perform a coherent Raman spectroscopy (e.g., stimulated Raman or coherent anti-Stokes Raman spectroscopy), and a radiation detector to detect radiation based on the spectroscopy. The chamber may have a resonant cavity to contain a sample for analysis, at least one window to the cavity to transmit the first radiation into the cavity and to transmit a second radiation out, a plurality of reflectors affixed to a housing of the cavity to reflect radiation of a predetermined frequency, the plurality of reflectors separated by a distance that is sufficient to resonate the radiation. The spectroscopic analysis system may be coupled with a nucleic acid sequencing system to receive a single nucleic acid derivative in solution and identify the derivative to sequence the nucleic acid.

Cars microscopy and spectroscopy using ultrafast chirped pulses

Linear chirped pulses in a Raman excitation scheme provide selective excitation of only one target transition (single mode) in a molecule without disturbing any other transitions or molecules. Selectivity is guaranteed by the adiabaticity of the pulse excitation, which allows manipulation by only a resonant mode while leaving all of the other modes unperturbed. This in turn allows for enhanced imaging or spectroscopic analysis of a sample that contains one or more of the molecules.

Detecting low-volatility chemicals buried below the ground using an acoustic emitter to generate vapour to be detected by an optical sensor

According to an embodiment, a system includes an acoustic emitter 120, a controller, an optical sensor 140, and an indicator for indicating a detection of a predetermined trace chemical vapour 20 by the optical sensor. The acoustic emitter is positioned at a predetermined distance above the ground surface and configured to project a beam of acoustic energy toward the ground surface with a variable angle of incidence. The controller is configured to control the acoustic emitter to vary the angle of incidence of the acoustic beam within the variable angle of incidence α, while the optical sensor is configured for the standoff sensing of a trace chemical vapour proximate the ground surface. Excitation of the ground surface, particularly at a critical angle of incidence, causes the release of trace chemical vapours from a buried source and the soil into the air above the buried source.

Frequency comb for downhole chemical sensing

The present disclosure relates to systems and methods for analyzing fluids. The method for analyzing a chemical sample within a wellbore, contained within an interrogation device, may comprise broadcasting a coherent light from a frequency comb module, directing the coherent light through a fiber optic line to the interrogation device, irradiating the chemical sample with the coherent light, capturing light resulting from the irradiation of the chemical sample, and producing a spectrum resulting from the captured light from the chemical sample. A frequency comb system for analyzing a chemical sample may comprise a frequency comb module configured to broadcast a coherent light and a fiber optic line that extends into a wellbore to an interrogation device. The interrogation device may further be configured to contain the chemical sample for irradiation by the coherent light. The frequency comb system may further comprise a receiver and an information handling system. <Figure 1>