Method for highly-sensitive and rapid detection of pesticide residue based on imprinted metal-organic framework probe

A method for detection of pesticide residue based on an imprinted metal-organic framework (MOF) probe. The method constructs a colorimetric test strip based on an MOF enzyme-mimic probe, where a molecularly imprinted MOF probe is used as a colorimetric probe to catalyse the oxidation of a substrate, thereby enabling colour change; filter paper is used as a substrate for supporting the colorimetric probe, including a quality control zone, standard zone, and detection zone; in the quality control zone, the optimal colorimetric analysis parameters are selected according to an environment to be tested; the standard zone is a standard colorimetric zone obtained through dropwise addition of standards with different concentrations and is provided to establish a colorimetric analysis mathematical model; and the detection zone is provided for detection of an actual sample. A concentration range of a pesticide residue in a test sample can be preliminarily determined by the colorimetric test strip ...

ANALYSIS DEVICE

Molecular diagnostic assay device and method of use

Lateral flow devices and methods of use for a molecular diagnostic assay are provided. The method is suitable for detection or monitoring of targets, including biological, chemical, and material targets that exist in very low concentrations in biological samples. The methods and devices of the present application are amenable to power source-free point of care testing.

Method for detection of (legionella) bacteria employing purified antigen-specific antibodies

DEVICE FOR USE IN THE DETECTION OF BINDING AFFINITIES

A device for use in the detection of binding affinities, the device comprising a planar waveguide (2) arranged on a substrate (3), and further comprising an optical coupler (41) having a predetermined length for coupling coherent light (1) of a predetermined wavelength into the planar waveguide (2) such that a parallel beam of coherent light propagates through the planar waveguide (2) with an evanescent field (11) of the coherent light propagating along an outer surface (21) of the planar waveguide (2). The outer surface (21) of the planar waveguide (2) comprises binding sites thereon capable of binding target samples to the binding sites such that light of the evanescent field (11) is diffracted by target samples bound to the binding sites. The binding sites are arranged along a plurality of predetermined straight lines (7) running parallel to one another with a constant distance between adjacent straight lines. The predetermined straight lines (7) are arranged at an angle relative to the direction of propagation of the evanescent field (11) such that the coherent light (12) diffracted by the target samples bound to the binding sites impinges under a diffraction angle relative to the straight lines onto a further optical coupler (8) arranged in a portion (10) of the planar waveguide (2) outside the beam of coherent light propagating through the planar waveguide. The further optical coupler (8) couples the diffracted coherent light (13) out of the planar waveguide (2) such as to interfere at a predetermined detection location (9) with a difference in optical path length which is an integer multiple of the predetermined wavelength.

Accurate Colorimetric Based Test Strip Reader System

Techniques for colorimetric based test strip analysis and reader system are provided. In one aspect, a method of test strip analysis includes: illuminating a test strip wetted with a sample with select spectrums of light, wherein the test strip includes test pads that are configured to change color in the presence of an analyte in the sample; obtaining at least one digital image of the test strip; and analyzing color intensity from the at least one digital image against calibration curves to determine an analyte concentration in the sample with correction for one or more interference substances in the sample that affect the color intensity. A calibration method and a reader device are also provided.

ANALYTICAL APPARATUS

ANALYSIS DEVICE AND ANALYSIS METHOD

A METHOD FOR DETECTING OPTICAL PHASE CHANGES DURING BIOSENSOR OPERATION,BIOSENSING APPARATUS AND A BIOSENSOR ADAPTED FOR USE IN THE SAME

DEVICE FOR USE IN THE DETECTION OF BINDING AFFINITIES

A device for use in the detection of binding affinities, the device comprising a planar waveguide (2) arranged on a substrate (3), and further comprising an optical coupler (41) having a predetermined length for coupling coherent light (1) of a predetermined wavelength into the planar waveguide (2) such that a parallel beam of coherent light propagates through the planar waveguide (2) with an evanescent field (11) of the coherent light propagating along an outer surface (21) of the planar waveguide (2). The outer surface (21) of the planar waveguide (2) comprises binding sites thereon capable of binding target samples to the binding sites such that light of the evanescent field (11) is diffracted by target samples bound to the binding sites. The binding sites are arranged along a plurality of predetermined straight lines (7) running parallel to one another with a constant distance between adjacent straight lines. The predetermined straight lines (7) are arranged at an angle relative to the direction of propagation of the evanescent field (11) such that the coherent light (12) diffracted by the target samples bound to the binding sites impinges under a diffraction angle relative to the straight lines onto a further optical coupler (8) arranged in a portion (10) of the planar waveguide (2) outside the beam of coherent light propagating through the planar waveguide. The further optical coupler (8) couples the diffracted coherent light (13) out of the planar waveguide (2) such as to interfere at a predetermined detection location (9) with a difference in optical path length which is an integer multiple of the predetermined wavelength.

Flame spectrophotometric detection of elements e.g. chlorine - using variable reduced pressure conditions in the burner

Le procédé selon l'invention consiste à réaliser, à l'intérieur d'un brûleur (1) dont la sortie est maintenue en dépression, deux combustions successives dans un flux d'hydrogène et à faire réagir l'élément recherché contenu dans les gaz issus de la première combustion avec un élément réactif, de manière à ce que la deuxième combustion produise une émission lumineuse caractéristique de l'élément recherché, ce procédé comportant une phase transitoire de mise en route comportant une réduction progressive de la dépression de manière à provoquer l'amorçage de la deuxième combustion, puis une augmentation progressive de la dépression jusqu'à ce qu'elle atteigne sa valeur nominale. L'invention s'applique à la détection d'éléments tels que du chlore.

Sample container

A fluid testing apparatus including a specimen cup and a cover having a reagent portion which is color sensitive to an analytical characteristic.

УСТРОЙСТВО ДЛЯ ПРИМЕНЕНИЯ ПРИ ДЕТЕКТИРОВАНИИ СРОДСТВА К СВЯЗЫВАНИЮ

FIELD: physics. SUBSTANCE: device includes a substrate, a planar waveguide located on the substrate, and two optical isolations. The first optical isolation is designed to insert coherent light into the waveguide. The outer surface of the planar waveguide contains binding centres on itself, capable of connecting the specified samples with the binding centres so that field light of damped oscillations is refracted by the specified samples connected with the binding centres. The binding centres are arranged along a plurality of lines placed parallel to one another, with the distance between adjacent straight lines being constant. The lines are placed at an angle relative to the field distribution of damped oscillations so that the coherent light refracted by the specified samples connected with the binding centres falls on the second optical isolation. The second optical isolation outputs the refracted coherent light from the planar waveguide in such a way that it interferes at a predetermined detection location. EFFECT: increased sensitivity of the device. 15 cl, 13 dwg РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 638 577 C2 (51) МПК G01N 21/77 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ ИЗОБРЕТЕНИЯ К ПАТЕНТУ (21)(22) Заявка: 2015125027, 03.12.2013 (24) Дата начала отсчета срока действия патента: 03.12.2013 (72) Автор(ы): ФАТТИНГЕР Кристоф (CH) (73) Патентообладатель(и): Ф. ХОФФМАНН-ЛЯ РОШ АГ (CH) Дата регистрации: (56) Список документов, цитированных в отчете о поиске: US 7505641 B1, 17.03.2009. GB Приоритет(ы): (30) Конвенционный приоритет: 2342440 A, 12.04.2000. US 5455178 A1, 03.10.1995. RU 2141645 C1, 20.11.1999. 04.12.2012 EP 12195532.2 (45) Опубликовано: 14.12.2017 Бюл. № 35 (85) Дата начала рассмотрения заявки PCT на национальной фазе: 06.07.2015 (86) Заявка PCT: EP 2013/075408 (03.12.2013) (87) Публикация заявки PCT: 2 6 3 8 5 7 7 (43) Дата публикации заявки: 10.01.2017 Бюл. № 1 R U 14.12.2017 2 6 3 8 5 7 7 R U Адрес для ...

Device

Molecular diagnostic assay device and method of use

Lateral flow devices and methods of use for a molecular diagnostic assay are provided. The method is suitable for detection or monitoring of targets, including biological, chemical, and material targets that exist in very low concentrations in biological samples. The methods and devices of the present application are amenable to power source-free point of care testing.

DIAGNOSTIC TESTING FOR IMMUNOLOGIC FOOD SENSITIVITY

DIAGNOSTIC TESTING FOR IMMUNOLOGIC FOOD SENSITIVITY In various implementations, a noninvasive, food sensitivity test may be utilized to identify one or more food sensitivities. A fecal sample may be obtained. The food sensitivity test may be performed on the obtained fecal sample. One or more food sensitivities may be identified based on the food sensitivity test. Obtain fecal sample Test fecal sample using food sensitivity test Identify one or more food sensitivities based at least partially on the food sensitivity test ...

DEVICE FOR DETERMINING AT LEAST ONE ANALYTE CAPABLE OF BEING CONTAINED IN A LIQUID SAMPLE

La présente invention concerne un dispositif pour la détermination de la présence et/ou de la quantité d'au moins un analyte susceptible d'être contenu dans un échantillon liquide, comportant un moyen de diffusion capillaire (2) sur lequel sont matérialisées une zone (3) de dépôt de l'échantillon liquide, une zone amont (4) de libération et au moins deux zones de capture (5). Ce dispositif (1 ) comporte encore au moins une zone aval de libération (6) qui se situe en aval de l'une au moins desdites zones de capture (5), laquelle zone aval de libération (6) comprend au moins un réactif de détection conjugué à un marqueur visible et/ou mesurable; et le réactif de détection d'une zone de libération (4, 6) et/ou le réactif de capture de la ou des zones de capture (5) complémentaires, situées directement en aval de ladite zone de libération (4, 6), sont aptes à se lier spécifiquement avec ledit analyte et/ou à se lier spécifiquement l'un avec l'autre, pour former un complexe permettant la détermination ...

MOLECULAR DIAGNOSTIC ASSAY DEVICE AND METHOD OF USE

Lateral flow devices and methods of use for a molecular diagnostic assay are provided. The method is suitable for detection or monitoring of targets, including biological, chemical, and material targets that exist in very low concentrations in biological samples. The methods and devices of the present application are amenable to power source-free point of care testing.

Used for determining the at least one can be contained in an analyte in a liquid sample of the device

ANALYSIS KIT FOR IDENTIFICATION OF DRUGS

Analysis kit for identification of drugs, consisting of a shell-shaped upper part (), which is connected to a shell-shaped lower part () at one side, so that it is pivotable by means of a film hinge (), at least two separate holding chambers (-) suitable for holding a test fluid container () containing a test fluid are arranged in the lower part, wherein the respective test fluid container () is connected to a sample chamber (), which holds the drug () to be tested, by flow channels (-) in a fluid-conducting manner. 1. An analysis kit that cooperates with at least two test fluid containers to test drugs , said analysis kit comprising:a shell-shaped upper part having at least one side;a shell-shaped lower part having at least one side;a hinge that connects the one side of said shell-shaped upper part to the one side of said shell-shaped lower part, said shell-shaped upper part and said shell-shaped lower part being pivotal between an open position and a closed position;at least two holding chambers that are located in a space that is defined between said shell-shaped upper part and said shell-shaped lower part at times when said shell-shaped upper part and said shell-shaped lower part are in the closed position, each of said holding chambers being configured to receive a respective one of said test fluid containers;a drug sample chamber; andat least two flow channels, each of said flow channels corresponding to a respective one of said at least two holding chambers and defining a fluid tight connection between said respective flow chamber and said drug sample chamber.2. The analysis kit of wherein at least one of said shell-shaped upper part and said shell-shaped lower part includes at least one flexible cover such that a flexible cover corresponds to a respective one of each of said at least two holding chambers claim 1 , each of said flexible covers being movable into said space that is defined between said shell-shaped upper part and said shell-shaped lower part ...

ANALYTE DETECTION DEVICES AND METHODS WITH HEMATOCRIT/VOLUME CORRECTION AND FEEDBACK CONTROL

Disclosed are devices, arrangements and methods for quantifying the concentration of an analyte present in bodily fluid, including: an assay pad having at least one chemical reagent capable of producing a detectable signal in the form of a reaction spot upon reaction with the analyte; a light source; a detector array; a processor; and a memory in communication with the processor, the memory comprising: (a) at least one value indicative of one or more of: (i) the level of hematocrit contained in the sample; (ii) the volume of the sample applied to the assay pad; or (iii) imperfections present in the reaction spot; and (b) at least one algorithm for calculating the concentration of the analyte contained in the sample. 127-. (canceled)28. An arrangement for measuring the concentration of an analyte contained in a sample of body fluid , the arrangement comprising:an assay pad comprising at least one chemical reagent capable of producing a detectable signal in the form of a color change at a reaction spot formed upon reaction with the analyte;a light source;a detector array;a processor; and [ (i) the volume of the sample applied to the assay pad; and', '(ii) imperfections present in the reaction spot; and, '(a) at least one value derived from the color change at the reaction spot indicative of one or more of, '(b) at least one algorithm for calculating the concentration of the analyte contained in the sample using the at least one value., 'a memory in communication with the processor, the memory comprising29. The arrangement of claim 28 , wherein the assay pad comprises only a single chemical reagent.30. The arrangement of claim 28 , wherein the detector array comprises a plurality of pixels claim 28 , and wherein the at least one value derived from the color change at the reaction spot indicative of the volume of the sample applied to the assay pad is based on the number of pixels in the detector array that have detected the color change.31. The arrangement of claim 28 ...

ANALYSEVORRICHTUNG

System zum Chemilumineszenz-basierten Detektieren von Methicillin-resistentem Staphylococcus aureus

Die vorliegende Erfindung umfasst eine Vorrichtung und ein dazugehöriges Verfahren zum Bestimmen des Vorhandenseins oder Nichtvorhandensein von Methicillin-resistentem Staphylococcus aureus in einer Probe. Die Offenbarung umfasst die folgenden Elemente: (1) einen Lateral-Flow-Streifen für die mikrofluidische Manipulation einer Probe; (2) eine Kassettenvorrichtung, die den Lateral-Flow-Streifen beinhaltet und eine Schnittstelle mit einer Detektiervorrichtung ermöglicht; (3) einen Kassettenhandhaber; (4) eine Leuchtreagenz-Zuführvorrichtung; und (5) eine Detektiervorrichtung für das Detektieren von elektromagnetischer Strahlung, die in der Lage ist, chemilumineszente Strahlung vom Lateral-Flow-Streifen in eine Ausgabe für einen Benutzer umzuwandeln.

OPTICAL SENSING

Abdeckkappe

The invention relates to a covering cap (10) for detecting and administering substances. According to the invention, an indicator (11) is deposited on the surface of at least one wall of the covering cap (10) and/or embedded in the wall material in such a way that a substance (20) to be indicated travels from outside the cap (10) to the indicator (11); said indicator (11) is different from the material or material combination forming the cap (10), is sensitive to the substance (20) to be indicated, and permanently or temporarily changes its state from an initial state in the presence of the substance (20).

Food allergen detection methods and systems using molecularly imprinted polymers

Methods and devices for the detection of food allergens using molecularly imprinted polymers that are imprinted for a target food allergen. A molecularly imprinted polymer may be imprinted using surface imprinting or other procedures. Detection of food allergens, such as peanut allergens, may be accomplished using all or a portion of a protein food allergen as a template to produce a molecularly imprinted polymer for food allergen detection. A portion utilized can be one that creates receptor sites in the molecularly imprinted polymer that are unique or more unique to the target food allergen than receptor sites that would be created if an entire food allergen molecule were utilized.

IMMUNOASSAY

POINT OF CARE ANALYTICAL PROCESSING SYSTEM

A point of care testing system includes a reader having an incubator disposed within a reader housing, the incubator having a rotor supported for rotation and having a plurality of circumferentially disposed slots. A drive mechanism is configured to rotate the rotor about a center axis A plurality of analytical test elements are sized for fitting in the slots of the incubator either manually or on demand. Each analytical test element commonly includes a support within a cartridge. The support retains at least one of a dry chemistry chip comprising a porous spreading layer disposed in stacked relation with at least one reagent layer or a lateral flow assay device wherein the plurality of test elements can assume a common form factor with multiplexed capability, and in which cartridges are preferably gated to enable random access processing.

OPTICAL SENSOR

... 2101421 9306463 PCTABS00021 An optical sensor senses the presence of an analyte in a fluid. The sensor has a surface plasmon resonance-sensitive device (17) which reflects light from a light source (11) to a light detector (34). A ''pinhole'' aperture (33) determines the portion of the reflected light which is permitted to reach the light detector. On exposure to the analyte the device (17) responds so as to alter the intensity of the light reflected to the light detector (34). The detector produces an output signal representative, for example, of the concentration of the analyte. The concentration is indicated by an indicating means (35).

LATERAL FLOW ASSAY DEVICES AND METHOD OF USE

Devices and Formulations for Detecting, Screening and Monitoring Levels of Certain Constituents in Bodily Fluids and Method

A device is disclosed for conducting a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid. An indicator formulation of the device changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A carrier substrate of the device is constructed of a material having voids providing a high void volume within the substrate. The device is made by applying a chromagen to the carrier substrate to create a chromagen-laden carrier member. Then, a selected reagent having a particular constituent-specific formulation is applied to the chromagen-laden member. The selected reagent then combines with the chromagen thereby establishing the indicator formulation within the carrier substrate in place for reception of a sample of the bodily fluid.

Methods and Systems for Analyzing a Liquid Medium

Methods and systems for colorimetrically analyzing a liquid medium by analyzing chemical test strip images are provided. The liquid medium can be industrial water in an industrial water system. Image analyzing software carries out the analysis. The results of the analysis can be used to diagnosing a chemical treatment regimen of the industrial water system. A chemical test strip holder can be used to enhance reliability and repeatability of the imaging process and/or subsequent analysis. 1. A method of determining concentration of a chemical species in a liquid medium , the method comprising:exposing a reactive zone of a chemical test strip to the liquid medium thereby creating a post-exposure reactive zone;imaging the post-exposure reactive zone using an imaging device thereby creating a digital image of the post-exposure reactive zone;optionally cropping the digital image of the post-exposure reactive zone to isolate a portion of the digital image for analysis;analyzing a colorimetric parameter of at least the portion of the digital image of the post-exposure reactive zone using the image analyzing software to determine the concentration of the chemical species;optionally outputting the determined concentration of the chemical species of the liquid medium.2. The method of claim 1 , wherein the liquid medium is water.3. The method of claim 2 , wherein the chemical species is selected from dissolved calcium claim 2 , acidity claim 2 , total hardness claim 2 , ortho-phosphate claim 2 , m-alkalinity claim 2 , p-alkalinity claim 2 , an oxidizing biocide claim 2 , or a non-oxidizing biocide.4. The method of claim 3 , wherein the chemical species is an oxidizing biocide.5. The method of claim 4 , wherein the oxidizing biocide comprises one or more stabilized oxidant or halogenated oxidant.6. The method of claim 4 , wherein the oxidizing biocide is a halogenated oxidant.7. The method of claim 6 , wherein the halogenated oxidant is selected from chlorine bleach claim 6 , ...

Method for Detection of Legionella Bacteria Employing Purified Antigen-Specific Antibodies

The present invention involves extracting from Legionella bacteria, particularly L. pneumophila bacteria, an essentially protein-free O-polysaccharide or carbohydrate antigen, coupling this antigen to an activated chromatographic column through a protein space molecule which is first conjugated to the antigen, utilizing the column thus prepared for the affinity purification of raw polyvalent antibodies to the same Legionella bacterium from which the O-polysaccharide or carbohydrate antigen was separated—thereby obtaining antigen-specific antibodies which are useful for the rapid detection of the corresponding Legionella bacterium or its antibody in human bodily fluids such as urine, sputum, blood and the like or in environmental samples suspected of harboring the Legionella bacterium.

УСТРОЙСТВО ДЛЯ ПРИМЕНЕНИЯ ПРИ ДЕТЕКТИРОВАНИИ СРОДСТВА К СВЯЗЫВАНИЮ

РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2015 125 027 A (51) МПК G01N 21/77 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21)(22) Заявка: 2015125027, 03.12.2013 (71) Заявитель(и): Ф. ХОФФМАНН-ЛЯ РОШ АГ (CH) Приоритет(ы): (30) Конвенционный приоритет: (72) Автор(ы): ФАТТИНГЕР Кристоф (CH) 04.12.2012 EP 12195532.2 (85) Дата начала рассмотрения заявки PCT на национальной фазе: 06.07.2015 R U (43) Дата публикации заявки: 10.01.2017 Бюл. № 01 (86) Заявка PCT: (87) Публикация заявки PCT: WO 2014/086789 (12.06.2014) A Адрес для переписки: 197046, Санкт-Петербург, Каменноостровский пр-кт, 1-3, оф. 30, ООО "Юридическая фирма Городисский и Партнеры" R U (57) Формула изобретения 1. Устройство для применения в детектировании сродства к связыванию, содержащее планарный волновод (2) расположенный на подложке (3), и дополнительно содержащее оптическую развязку (41), имеющую заранее определенную длину для введения когерентного света (1) заранее определенной длины волны в планарный волновод (2) таким образом, чтобы когерентный свет распространялся через планарный волновод (2) с полем (11) затухающих колебаний параллельно с когерентным светом, распространяющимся вдоль внешней поверхности (21) планарного волновода (2), содержащей на ней центры (5) связывания, способные к связыванию заданных образцов (6) с центрами (5) связывания, таким образом, чтобы свет поля (11) затухающих колебаний преломлялся заданными образцами (6), связанными с центрами (5) связывания, в особенности, с центрами (5) связывания, расположенными вдоль множества заранее определенных линий (7), проложенных взаимно параллельно, имеющих постоянное расстояние между прилежащими прямыми линиями, а именно заранее определенными линиями из множества определенных линий (7), расположенных под углом (β) относительно направления распространения поля (11) затухающих колебаний, чтобы когерентный свет (12), преломленный заданными образцами (6), связанными с центрами (5) связывания падал ...

АНАЛИТИЧЕСКАЯ ОБРАБАТЫВАЮЩАЯ СИСТЕМА В МЕСТЕ НАБЛЮДЕНИЯ

CONSUMER-BASED DISEASE DIAGNOSTICS

A diagnostic system performs a disease diagnostic test using at least an optical property modifying device and a mobile device. A user provides a biological sample to the optical property modifying device. The biological sample reacts with a reagent in reaction chambers of the device. The user captures images of the optical property modifying device using the mobile device. Based on an analysis of the captured images, the diagnostic system can determine the result of the disease diagnostic test. The diagnostic system presents the test result to the user via the mobile device.

DEVICE FOR DETERMINING AT LEAST ONE ANALYTE THAT MAY BE CONTAINED IN A LIQUID SAMPLE

La présente invention concerne un dispositif pour la détermination de la présence et/ou de la quantité d'au moins un analyte susceptible d'être contenu dans un échantillon liquide, comportant un moyen de diffusion capillaire (2) sur lequel sont matérialisées une zone (3) de dépôt de l'échantillon liquide, une zone amont (4) de libération et au moins deux zones de capture (5). Ce dispositif (1) comporte encore au moins une zone aval de libération (6) qui se situe en aval de l'une au moins desdites zones de capture (5), laquelle zone aval de libération (6) comprend au moins un réactif de détection conjugué à un marqueur visible et/ou mesurable ; et le réactif de détection d'une zone de libération (4, 6) et/ou le réactif de capture de la ou des zones de capture (5) complémentaires, situées directement en aval de ladite zone de libération (4, 6), sont aptes à se lier spécifiquement avec ledit analyte et/ou à se lier spécifiquement l'un avec l'autre, pour former un complexe permettant la détermination ...

MOLECULAR DIAGNOSTIC ASSAY DEVICE AND METHOD OF USE

Fluorometric system, method and test article

A fluorometric system to determine the kind and amount of substances derived from a biological fluid (e.g., serum or urine) or tissue in which the substances to be detected (e.g., antigen, antibody, hormone or enzyme) are coated onto a substrate surface in fluorescent form. Multiple coating areas of different samples may be employed. The fluorometric system includes a source of filtered light to excite fluorescence, an optical system for conducting the excitation light to such coating, and optical systems for receiving emitted fluorescence and for detecting the same. The system efficiency and optical characteristics disclosed avoid photo-bleaching; limit fading; and are especially adapted to provide accurate surface reading fluorometry.

Optical sensor

PCT No. PCT/GB92/01723 Sec. 371 Date Nov. 10, 1993 Sec. 102(e) Date Nov. 10, 1993 PCT Filed Sep. 18, 1992 PCT Pub. No. WO93/06463 PCT Pub. Date Apr. 1, 1993.An optical sensor assembly includes a light source, a surface plasmon-sensitive structure for reflecting light, a light detector, and a signal indicator. The light detector receives light that is reflected from the surface plasmon-sensitive structure at an angle which is sensitive to surface plasmon absorption.

Multi-Dimensional Cross-Reactive Array for Chemical Sensing

The discrimination ability of a chemical sensing cross-reactive arrays is enhanced by constructing sensing elements in two dimensions, first in the x-y plane of the substrate, second in the z dimension so that the sensors are vertically stacked on top of one another. Stacking sensing elements on top of one another adds to the discrimination ability by enabling the characteristic measurement of how fast target chemicals are passing through the stack of sensors. The new invention also allows the ability to discriminate components in a sample mixture by separating them using their innate difference in diffusional rates. Multi-sensor response patterns at each z level of sensors and time delay information from the sample passing from one level to the next are used to generate the response vector. The response vector is used to identify individual component samples and components in a mixture sample.

Measuring chamber, working method of measuring chamber, chemiluminescence measurement method of measuring chamber and chemiluminescence detector

The present disclosure relates to a measuring chamber, a working method of the measuring chamber, a chemiluminescence measurement method of the measuring chamber and a chemiluminescence detector. The measuring chamber includes a dark chamber, a first substrate nozzle, a photomultiplier detection component, a waste liquor adsorption needle component, a reaction cup turntable and a plurality of reaction cup processing stations; the reaction cup turntable is provided in the measuring chamber rotationally; and the plurality of reaction cup processing stations are sealed in a mutually light-isolated manner. When the instrument works, reaction cups in the reaction cup turntable are moved in the dark chamber; and after the reaction cups are moved to corresponding processing stations for processing the reaction cups, the plurality of different processing stations for processing, the reaction cups may simultaneously process the reaction cups moved to the corresponding reaction cup processing stations ...

Verfahren zur Herstellung eines Chemosensors

Optical sensing

OPTICAL SENSOR AND METHODS FOR MEASURING MOLECULAR BINDING INTERACTIONS

Analytical apparatus

ANALYTE DETECTION DEVICES AND METHODS WITH HEMATOCRIT/VOLUME CORRECTION AND FEEDBACK CONTROL

... ²An arrangement may include an assay pad comprising at least one chemical ²reagent capable of producing a detectable signal in the form of a reaction ²spot upon ²reaction with the analyte; a light source; a detector array; a processor; and ²a memory ²in communication with the processor that includes at least one value ²indicative of ²one or more of: (i) the level of hematocrit in the sample; (ii) the volume of ²the ²sample applied to the assay pad; or (iii) imperfections in the reaction spot; ²and at ²least one algorithm for calculating a concentration of an analyte. ²Alternatively, an ²arrangement may include an assay pad comprising at least one chemical reagent ²capable of producing a detectable signal in the form of a reaction spot; a ²detector ²array; a processor; a memory; and at least one catalyst device constructed and ²²arranged to be responsive to control signals.² ...

IMMUNOASSAY

IMMUNOASSAY Abstract of the Disclosure Apparatus and method for providing an immunoassay of a binding reaction between a ligand and an antiligand which are typically an antigen and an antibody, including a spatial pattern formed by a spatial array of separate regions of antiligand material, and ligand material dispersed to interact with the spatial array of separate regions of antiligand material for producing a binding reaction between the ligand and the antiligand in the spatial patterns and with the bound complexes labeled with a particular physical characteristic. A source of input energy and with the input energy at a particular spectrum for interacting with particular physical characteristic of the labeled binding reaction. Scanning the spatial pattern with the input energy at the particular spectrum for producing output energy having amplitude levels formed by a substantially random background component and a non-random component representing the labeled bound complexes, and the non random component representing the labeled bound complexes detected to produce an output signal in accordance with the labeled binding reaction. ************

MULTI-FLUID TEST STRIP

A multi-fluid test strip may include a first zone to test a first fluid, a second zone to test a second fluid, and a third zone intermediate the first zone and the second zone. The third zone may prevent cross-contamination of the first fluid with the second zone and prevent cross-contamination of the second fluid with the first zone. The multi-fluid test strip may also include a first grip zone and a second grip zone. The first zone may be intermediate the first grip zone and the third zone and the second zone may be intermediate the second grip zone and the third zone. The first grip and second grip zones may be dimensioned to permit gripping of the first and second grip zones without touching the first or second zones.

FAST FLOW APPARATUS AND METHOD TO EVALUATE ANALYTES IN LIQUID, SOLID AND SEMI-SOLID SAMPLES

Disclosed is a fast flow device, for detecting an analyte in a sample, comprising a grooved sample pad for collecting the sample, a test strip coupled to the sample pad, and a housing enclosing and allowing visualization of the test strip. The sample pad can include markings for determining the amount of sample collected. The device can also preferably include a vented flexible collar connecting the housing to a run fluid container, which vented flexible collar fits over the sample pad and forms an air-tight seal between the housing and the run fluid container. The device achieves fast flow because the venting equalizes internal pressure of the housing and run fluid container and because of the grooving on the sample pad. Further, the device can include a second sample pad/test strip combination for detection of a second analyte. Also disclosed are methods for detecting an analyte in a sample using the device.

Devices and Formulations for Detecting, Screening and Monitoring Levels of Certain Constituents in Bodily Fluids and Method

A device is disclosed for conducting a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid. An indicator formulation of the device changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A carrier substrate of the device is constructed of a material having voids providing a high void volume within the substrate. The device is made by applying a chromagen to the carrier substrate to create a chromagen-ladencarrier member. Then, a selected reagent having a particular constituent-specific formulation is applied to the chromagen-laden member. The selected reagent then combines with the chromagen thereby establishing the indicator formulation within the carrier substrate in place for reception of a sample of the bodily fluid.

Accurate colorimetric based test strip reader system

ANALYTICAL APPARATUS

Analytical apparatus includes a pyroelectric or other thermo-electric transducer element (10) in strip form. thin film electrodes (12,14) are provided and one or more dots of reagent (16) are deposited on the transducer surface. A small amount of biological or other sample is deposited over the reagent dots which undergo selective colorimetric changes. The transducer is illuminated from below by LED light sources (24) and light absorption in the reagent regions is detected as microscopic heating at the transducer surface. The electrical signal output from the pyroelectric transducer is processed to derive analyte information.

MISCH- UND TRANSFERVORRICHTUNG FÜR MATERIALIEN, DIE IN BIOLOGISCHEN UND BIOCHEMISCHEN ASSAYS VERWENDET WERDEN

Eine Mischvorrichtung zum Bearbeiten biologischer, chemischer oder biochemischer Materialien, die in einem Assay verwendet werden, umfasst ein Mischelement, das mit einer Vielzahl von Kammern ausgebildet ist, die jeweils eine entlang einer Kante des Mischelements vorgesehene verschließbare Öffnung haben. Die Mischvorrichtung umfasst auch ein oder mehrere Kompartimente, die entlang der Kante des Mischelements zwischen ausgewählten der geschlossenen Kammern bewegbar sind. Dieses Kompartiment dient dazu, Materialien von den Kammern zu empfangen, und zwischen den Kammern zu transferieren. Ausgewählte der Kammern umfassen zugehörige Verarbeitungselemente, beispielsweise einschließlich von Heiz- und Kühlelementen, magnetischen Elementen, Membranen und Lateral-Flow-Vorrichtungen. Die Mischvorrichtung ist auch schwenkbar, beispielsweise um die Anwendung von Schwerkraft im Transfer von Materialien zwischen den Kammern und einem oder mehreren Kompartimenten zu erleichtern. Die Mischvorrichtung kann ...

Lateral flow assay devices and method of use

The present invention relates to testing biological or industrial samples. Disclosed by preferred embodiments is an electronic assay test reader for reading a lateral flow test strip having a development area comprising a test background region and at least one test result line, the electronic lateral flow assay test reader comprising: a cassette for retaining the test strip and a carrier adapted to removably retain the cassette therein; at least one illumination LED operably associated with one or a combination of the cassette and the carrier for illuminating the test strip, and; a light guide comprising a window structure of one or a combination of the cassette and the carrier to direct light emitted or reflected from a selected portion of the development area of the test strip to a sensor wherein the proportion of the at least one test result line relative to the proportion of test background region in the selected portion of the development area of the test strip is maximised.

Optical sensor and methods for measuring molecular binding interactions

FOOD ALLERGEN DETECTION METHODS AND SYSTEMS USING MOLECULARLY IMPRINTED POLYMERS

Methods and devices for the detection of food allergens using molecularly imprinted polymers that are imprinted for a target food allergen. A molecularly imprinted polymer may be imprinted using surface imprinting or other procedures. Detection of food allergens, such as peanut allergens, may be accomplished using all or a portion of a protein food allergen as a template to produce a molecularly imprinted polymer for food allergen detection. A portion utilized can be one that creates receptor sites in the molecularly imprinted polymer that are unique or more unique to the target food allergen than receptor sites that would be created if an entire food allergen molecule were utilized.

DIAGNOSTIC TESTING FOR IMMUNOLOGIC FOOD SENSITIVITY

In various implementations, a noninvasive, food sensitivity test may be utilized to identify one or more food sensitivities. A fecal sample may be obtained. The food sensitivity test may be performed on the obtained fecal sample. One or more food sensitivities may be identified based on the food sensitivity test.

DEVICE FOR DETERMINING AT LEAST ONE ANALYTE CAPABLE OF BEING CONTAINED IN A LIQUID SAMPLE

L'invention concerne un dispositif pour la détermination de la présence et/ou quantité d'au moins un analyte susceptible d'être contenu dans un échantillon liquide, mettant en oeuvre une technique immunochromatographique par migration latérale répondant aux problèmes générés par l'excès ou le mélange de réactifs de détection rapportés en amont d'une pluralité de zones de capture successives, ce mélange n'étant pas satisfaisant à cause de réactions croisées entre les différents réactifs de détection et l'inhibition d'activité spécifique mutuelle entre les réactifs. Le réactif de détection et/ou le réactif de capture sont aptes à se lier spécifiquement avec l'analyte et/ou à se lier spécifiquement l'un avec l'autre pour former un complexe permettant la détermination de l'analyte dans l'échantillon liquide au niveau des zones de capture complémentaires. Le dispositif comprend une zone de libération matérialisée sur un moyen de diffusion capillaire et se situant en aval de la ou des zones de capture.

MULTI-FLUID STRIP TEST

A multi-fluid test strip may include a first zone to test a first fluid, a second zone to test a second fluid, and a third zone intermediate the first zone and the second zone. The third zone may prevent cross-contamination of the first fluid with the second zone and prevent cross-contamination of the second fluid with the first zone. The multi-fluid test strip may also include a first grip zone and a second grip zone. The first zone may be intermediate the first grip zone and the third zone and the second zone may be intermediate the second grip zone and the third zone. The first grip and second grip zones may be dimensioned to permit gripping of the first and second grip zones without touching the first or second zones.

Measuring Chamber, Working Method of Measuring Chamber, Chemiluminescence Measurement Method of Measuring Chamber and Chemiluminescence Detector

The present disclosure relates to a measuring chamber, a working method of the measuring chamber, a chemiluminescence measurement method of the measuring chamber and a chemiluminescence detector. The measuring chamber includes a dark chamber, a first substrate nozzle, a photomultiplier detection component, a waste liquor adsorption needle component, a reaction cup turntable and a plurality of reaction cup processing stations; the reaction cup turntable is provided in the measuring chamber rotationally; and the plurality of reaction cup processing stations are sealed in a mutually light-isolated manner. When the instrument works, reaction cups in the reaction cup turntable are moved in the dark chamber; and after the reaction cups are moved to corresponding processing stations for processing the reaction cups, the plurality of different processing stations for processing, the reaction cups may simultaneously process the reaction cups moved to the corresponding reaction cup processing stations ...

Accurate colorimetric based test strip reader system

Techniques for colorimetric based test strip analysis and reader system are provided. In one aspect, a method of test strip analysis includes: illuminating a test strip wetted with a sample with select spectrums of light, wherein the test strip includes test pads that are configured to change color in the presence of an analyte in the sample; obtaining at least one digital image of the test strip; and analyzing color intensity from the at least one digital image against calibration curves to determine an analyte concentration in the sample with correction for one or more interference substances in the sample that affect the color intensity. A calibration method and a reader device are also provided.

Method for detection of (legionella) bacteria employing purified antigen-specific antibodies

Testing device for identifying antigens and antibodies in biofluids

A testing device for identifying an antigen or antibody within a biofluid sample including: a substrate having a hydrophilic surface thereon; the surface including a collection zone, and at least one detection zone extending therefrom; wherein the biofluid sample can be mixed with a specific antigen or antibody, and deposited on the collection zone and transferred by capillary action to the detection zone; the antigen or antibody in the biofluid sample reacting with an appropriate said antibody or antigen thereby resulting in a visual indication within the detection zone.

METHOD AND DEVICE FOR DETECTING, BY MEANS OF FLAME SPECTROPHOTOMETRY, ELEMENTS SUCH AS CHLORINE IN A GASEOUS COMPOSITION.

SAMPLE MIXING DEVICE AND SPECIFIC COMPONENT DETECTING DEVICE HAVING SAME

According to the present invention, provided are a sample mixing device and a specific component detecting device having the same. The sample mixing device comprises: a sample chamber having a space to which a sample is able to be introduced; a detection reagent chamber formed on one side surface of the sample chamber, containing a detection reagent mixed with the sample to form a light emitting substance; a waterproof diaphragm which blocks a space between the sample chamber and the detection reagent chamber, and made of a waterproof material such that the sample and the detection reagent cannot penetrate through the waterproof diaphragm; an injection tube elongated from one side surface of the sample chamber such that the sample is able to be injected into the sample chamber; and a ventilation unit formed to be elongated from the sample chamber such that when the sample is injected into the sample chamber, air in the sample chamber is discharged through the ventilation unit. The waterproof ...

MOLECULAR DIAGNOSTIC ASSAY DEVICE AND METHOD OF USE

Lateral flow devices and methods of use for a molecular diagnostic assay are provided. The method is suitable for detection or monitoring of targets, including biological, chemical, and material targets that exist in very low concentrations in biological samples. The methods and devices of the present application are amenable to power source-free point of care testing.

Optical sensing

An optical sensor 10 senses the presence of an analyte in a fluid. The sensor has a surface plasmon resonance-sensitive device 17 which reflects light from a light source 11 to a light detector 34. A 'pinhole' aperture 33 determines the portion of the reflected light which is permitted to reach the light detector. On exposure to the analyte, via inlet pipe 24 and outlet pipe 25, the device 17 responds so as to alter the intensity of the light reflected to the light detector 34. The detector produces an output signal representative, for example, of the concentration of the analyte. The concentration is indicated by an indicating means 35. The 'pinhole' aperture 33 may be replaced by an optical fibre end 37 (fig. 3, not shown). ...

Accurate colorimetric based test strip reader system

ANALYTICAL APPARATUS

ANALYTICAL APPARATUS

Analytical apparatus includes a pyroelectric or other thermo-electric transducer element (10) in strip form. Thin film electrodes (12,14) are provided and one or more dots of reagent (16) are deposited on the transducer surface. A small amount of biological or other sample is deposited over the reagent dots which undergo selective colorimetric changes. The transducer is illuminated from below by LED light sources (24) and light absorption in the reagent regions is detected as microscopic heating at the transducer surface. The electrical signal output from the pyroelectric transducer is processed to derive analyte information.

LATERAL FLOW ASSAY DEVICES AND METHOD OF USE

The present invention relates to testing biological or industrial samples. Disclosed by preferred embodiments is an electronic assay test reader for reading a lateral flow test strip having a development area comprising a test background region and at least one test result line, the electronic lateral flow assay test reader comprising: a cassette for retaining the test strip and a carrier adapted to removably retain the cassette therein; at least one illumination LED operably associated with one or a combination of the cassette and the carrier for illuminating the test strip, and; a light guide comprising a window structure of one or a combination of the cassette and the carrier to direct light emitted or reflected from a selected portion of the development area of the test strip to a sensor wherein the proportion of the at least one test result line relative to the proportion of test background region in the selected portion of the development area of the test strip is maximised.

POINT OF CARE ANALYTICAL PROCESSING SYSTEM

A point of care testing system includes a reader having an incubator disposed within a reader housing, the incubator having a rotor supported for rotation and having a plurality of circumferentially disposed slots. A drive mechanism is configured to rotate the rotor about a center axis A plurality of analytical test elements are sized for fitting in the slots of the incubator either manually or on demand. Each analytical test element commonly includes a support within a cartridge. The support retains at least one of a dry chemistry chip comprising a porous spreading layer disposed in stacked relation with at least one reagent layer or a lateral flow assay device wherein the plurality of test elements can assume a common form factor with multiplexed capability, and in which cartridges are preferably gated to enable random access processing.

A diabetic nephropathy detection test paper

DEVICE FOR DETERMINING AT LEAST ONE ANALYTE THAT MAY BE CONTAINED IN A LIQUID SAMPLE

MOLECULAR DIAGNOSTIC ASSAY DEVICE AND METHOD OF USE

Lateral flow devices and methods of use for a molecular diagnostic assay are provided. The method is suitable for detection or monitoring of targets, including biological, chemical, and material targets that exist in very low concentrations in biological samples. The methods and devices of the present application are amenable to power source-free point of care testing.

Systems and methods for characterizing radioactive analytes

A device for automated characterization of radioactive analytes that provides an integrated system with liquid handling and plate reading components. The device can be further configured to include a chromatographic subsystem. Also provided is a method of using such a device, providing addition of a radioactive sample and a sequence of operations involving the abovementioned components of the system. The system is configured with radiation shielding in such a way that manipulations of radioactive samples do not interfere with concurrent radioactive measurements.

Optical sensor and methods for measuring molecular binding interactions

Methods and devices for the measurement of molecular binding interactions. Preferred embodiments provide real-time measurements of kinetic binding and disassociation of molecules including binding and disassociation of protein molecules with other protein molecules and with other molecules. In preferred embodiments ligands are immobilized within pores of a porous silicon interaction region produced in a silicon substrate, after which analytes suspended in a fluid are flowed over the porous silicon region. Binding reactions occur when analyte molecules diffuse closely enough to the ligands to become bound. Preferably the binding and subsequent disassociation reactions are observed utilizing a white light source and thin film interference techniques with spectrometers arranged to detect changes in indices of refraction in the region where the binding and disassociation reactions occur. In preferred embodiments both ligands and analytes are delivered by computer controlled robotic fluid flow ...

OPTICAL SENSING

Systems and methods for reagentless test strips

A system for detecting an analyte with a reagentless dry test strip includes a collector for collecting a blood sample from a user. The system additionally includes a mixer for receiving the collector and mixing the blood sample. The system additionally includes reagents, located in the mixer, for mixing with the blood sample. The system additionally includes a dry test strip for receiving the blood sample mixed with the reagents.

SYSTEMS AND METHODS FOR REAGENTLESS TEST STRIPS

A system for detecting an analyte with a reagentless dry test strip includes a collector for collecting a blood sample from a user. The system additionally includes a mixer for receiving the collector and mixing the blood sample. The system additionally includes reagents, located in the mixer, for mixing with the blood sample. The system additionally includes a dry test strip for receiving the blood sample mixed with the reagents.

Vorrichtung zur Bestimmung wenigstens eines Analyten, der in einer flüssigen Probe enthalten sein kann

Vorrichtung zur Bestimmung der Anwesenheit und/oder der Menge wenigstens eines Analyten, der in einer flüssigen Probe enthalten sein kann, umfassend eine Kapillardiffusionseinrichtung (2), auf der die flüssige Probe gemäß der Orientierung und Richtung der Kapillarmigration seitlich wandern soll und auf der sich in der Kapillarmigrationsrichtung von stromaufwärts nach stromabwärts wenigstens Folgendes befindet: eine Zone (3) zur Auftragung der flüssigen Probe; eine stromaufwärts gelegene Freisetzungszone (4), die wenigstens ein Nachweisreagens umfasst, das mit einem sichtbaren und/oder messbaren Marker konjugiert ist, wobei sich das Nachweisreagens als Folge der Migration der flüssigen Probe in der Kapillardiffusionseinrichtung (2) bewegen kann; und wenigstens zwei Abfangzonen (5), die jeweils wenigstens ein Abfangreagens umfassen, das auf der Kapillardiffusionseinrichtung (2) immobilisiert ist; dadurch gekennzeichnet, dass die Vorrichtung (1) weiterhin wenigstens eine stromabwärts gelegene ...

Device

A reading device 26 for a lateral flow test strip 2 includes a sample receiving volume 27 between first and second faces 28, 29. The sample receiving volume 27 is configured to receive at least a portion of a lateral flow test strip 2 between the first and second faces 28, 29. The reading device 26 includes a light emitter such as an LED to illuminate a reading portion 34 of the sample receiving volume 27. The reading device 26 also includes a photodetector 37 arranged to receive light from the reading portion 34 which may be transmitted through, reflected from or emitted from within the reading portion 34. A width of the reading portion 34 in the direction x of the longitudinal axis of the lateral flow immunoassay 2 is greater than a width of a test region 5 of the lateral flow device 2 in the same direction x.

dispositivos de ensaio de fluxo lateral e método de uso

A presente invenção refere-se ao teste de amostras biológicas ou industriais. É descrito por modalidades preferenciais um leitor de teste eletrônico para ler uma tira de teste de ensaio de fluxo lateral tendo uma área de desenvolvimento compreendendo uma região de fundo do teste e pelo menos uma linha de resultado do teste, o leitor de teste de ensaio de fluxo lateral eletrônico compreendendo: um cassete para reter a tira de teste e um suporte adaptado para reter de forma removível o cassete; pelo menos um LED de iluminação operacionalmente associado com um ou uma combinação do cassete e do suporte para iluminar a tira de teste, e; um guia de luz compreendendo uma estrutura de janela de um ou uma combinação do cassete e do suporte para direcionar a luz emitida ou refletida a partir de uma parte selecionada da área de desenvolvimento da tira de teste para um sensor, em que a proporção de pelo menos uma linha de resultado do teste em relação à proporção da região de fundo do teste na parte selecionada da área de desenvolvimento da tira de teste é maximizada. The present invention relates to testing biological or industrial samples. Described by preferred embodiments is an electronic test reader for reading a lateral flow test test strip having a development area comprising a test background region and at least one test result line, the test test test reader. electronic lateral flow comprising: a cassette for holding the test strip and a holder adapted to detachably retain the cassette; at least one LED illumination operatively associated with one or a combination of the cassette and holder for illuminating the test strip, and; a light guide comprising a window structure of one or a combination of cassette and holder for directing light emitted or reflected from a selected portion of the test strip development area to a sensor, wherein the proportion of hair minus one test result line in relation to the proportion of the test background region in the selected portion ...

Fluorometric system, method and test article

A fluorometric system to determine the kind and amount of substances derived from a biological fluid (e.g., serum or urine) or tissue in which the substances to be detected (e.g., antigen, antibody, hormone or enzyme) are coated onto a substrate surface in fluorescent form. Multiple coating areas of different samples may be employed. The fluorometric system includes a source of filtered light to excite fluorescence, optical systems for conducting the excitation light to such coating, and optical systems for receiving emitted fluorescence and for detecting the same. The system efficiency and optical characteristics disclosed avoid photo-bleaching; limit fading; and are especially adapted to provide accurate surface reading fluorometry.

VERFAHREN ZUR DETEKTION VON LEGIONELLA BAKTERIEN UNTER VERWENDUNG AUFGEREINIGTER ANTIGEN-SPEZIFISCHER ANTIKÖRPER

Testing device for identifying antigens and antibodies in biofluids

A testing device for identifying an antigen or antibody within a biofluid sample including: a substrate having a hydrophilic surface thereon; the surface including a collection zone, and at least one detection zone extending therefrom; wherein the biofluid sample can be mixed with a specific antigen or antibody, and deposited on the collection zone and transferred by capillary action to the detection zone; the antigen or antibody in the biofluid sample reacting with an appropriate said antibody or antigen thereby resulting in a visual indication within the detection zone.

ANALYTICAL APPARATUS

Food allergen detection methods and systems using molecularly imprinted polymers

Methods and devices for the detection of food allergens using molecularly imprinted polymers that are imprinted for a target food allergen. A molecularly imprinted polymer may be imprinted using surface imprinting or other procedures. Detection of food allergens, such as peanut allergens, may be accomplished using all or a portion of a protein food allergen as a template to produce a molecularly imprinted polymer for food allergen detection. A portion utilized can be one that creates receptor sites in the molecularly imprinted polymer that are unique or more unique to the target food allergen than receptor sites that would be created if an entire food allergen molecule were utilized.

Automated identification of assay areas in a microfluidic device and detection of assay positive areas based on rate of change of image light intensity

Methods are provided for the automated detection of assay-positive assay areas in a microfluidic device. When assays are performed in a microfluidic device, the configuration of the microfluidic circuit and its constituent circuit elements can determine where the reagents/analytes used in the assay can be located within the microfluidic circuit. Methods are provided for automatic identification of the size and shape of the assay areas based on a number of parameters which may include type of assay involved, shape and dimensions of microfluidic circuit elements, velocity and physical characteristics of the fluidic medium within the microfluidic circuit, physical/chemical properties of the analytes/reagents, and/or the number of cells being assayed.

Portable Devices for Detection of Antibodies Against Therapeutic Drugs

Portable devices for anti-drug antibodies (ADAs) testing are provided. These devices can be used in various applications, including but not restricted to the following: uniform testing of patients for ADAs; selection of therapeutic drug for patient treatment; evaluation of the need to change therapeutic drug or to apply tolerance regimens; selection of patients for clinical trials; comparison of therapeutic drugs marketed for a given disease and also gene therapy; scientific guidance for discovering therapeutic drugs; therapeutic drug development; postmarketing surveillance of therapeutic drugs. 1. A portable device for testing anti-drug antibodies (ADAs) in tissues and body fluids , comprising:a. A support that can be hand-held or rest on a surface;b. A sample-receiving port adjacent to a sample-colleting pad;c. A material containing labeled entities capable of binding to a constant region of ADAs; andd. A membrane containing a chimeric protein including a region from a therapeutic drug fused with an entity with low binding propensity for the labeled entities of step (c), wherein the region from a therapeutic drug contains one or more target antigens capable of binding to ADAs;2. The portable device of claim 1 , wherein the chimeric protein includes a region from a therapeutic drug capable of binding to anti-drug antibodies (ADAs) claim 1 , fused with a constant region of an antibody from a species different than the one producing the ADAs being tested.3. The portable device of claim 1 , wherein the membrane contains a variable region of a therapeutic antibody as a capture reagent claim 1 , and binding of that capture reagent to anti-drug antibodies results in a detectable signal.4. The portable device of having a code allowing access to a database.5. The portable device of claim 1 , wherein a filter is added upstream from or above the sample pad.6. The portable device of claim 1 , wherein a liquid reservoir is added upstream from or above the sample pad.7. The ...

DEVICE AND METHOD FOR DETERMINATION OF A CATALYST STATE IN A CHEMICAL REACTOR

DIAGNOSTIC TESTING FOR IMMUNOLOGIC FOOD SENSITIVITY

In various implementations, a noninvasive, food sensitivity test may be utilized to identify one or more food sensitivities. A fecal sample may be obtained. The food sensitivity test may be performed on the obtained fecal sample. One or more food sensitivities may be identified based on the food sensitivity test. 1. A diagnostic test kit to identify food sensitivity in humans , wherein the kit comprises: a substrate; and', 'a test region, wherein the test region comprises one or more antibody binding agents coupled to the substrate, wherein at least one of the antibody binding agents is adapted to couple to one or more first antibodies associated with one or more food sensitivities in the first set of food sensitivities when one or more of the first antibodies is present in a fecal sample obtained from the human;', 'wherein the diagnostic test is configured to contact the fecal sample of the human to allow identification of the presence of the first set of food sensitivities; and wherein one or more of the first antibodies, if present in the fecal sample, is transferred to the test region of the diagnostic test by the contact., 'a diagnostic test to identify the presence of a first set of food sensitivities in a human, wherein the diagnostic test comprises2. The kit of wherein the diagnostic test comprises a stick test claim 1 , and wherein the stick test comprises:a first end, wherein the first end comprises a handle to allow a user to hold the stick test without contacting the fecal sample; anda second opposing end, wherein the second opposing end contacts the fecal test to allow transfer of one or more of the first antibodies, if present in the fecal sample, to the test region of the diagnostic test.3. The kit of wherein the diagnostic test further comprises an absorbent region adapted to transfer a testing portion of a fecal sample from the fecal sample to the test region of the diagnostic test claim 1 , wherein the absorbent region contacts the fecal sample and ...

Methods and systems for analyzing a liquid medium

Methods and systems for colorimetrically analyzing a liquid medium by analyzing chemical test strip images are provided. The liquid medium can be industrial water in an industrial water system. Image analyzing software carries out the analysis. The results of the analysis can be used to diagnosing a chemical treatment regimen of the industrial water system. A chemical test strip holder can be used to enhance reliability and repeatability of the imaging process and/or subsequent analysis.

Solid-phase binding assay system and method for interferometrically measuring analytes bound to an active receptor

MULTI-LAYERED DEVICES FOR ANALYTE DETECTION

Devices and formulations for detecting, screening and monitoring levels of certain constituents in bodily fluids and method

A device is disclosed for conducting a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid. An indicator formulation of the device changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A carrier substrate of the device is constructed of a material having voids providing a high void volume within the substrate. The device is made by applying a chromagen to the carrier substrate to create a chromagen-laden carrier member. Then, a selected reagent having a particular constituent-specific formulation is applied to the chromagen-laden member. The selected reagent then combines with the chromagen thereby establishing the indicator formulation within the carrier substrate in place for reception of a sample of the bodily fluid.

Process and materials for the rapid detection of streptococcus pneumoniae employing purified antigen-specific antibodies

A process is disclosed for obtaining a C-polysaccharide cell wall antigen containing not more than about 10% protein from Streptococcus pneumoniae bacteria. The antigen thus obtained is conjugated to a spacer molecule, and the free end of the latter is then conjugated to a chromatographic affinity column. The column is then utilized to purify raw antibodies to S. pneumonia bacteria, thereby producing antigen-specific antibodies. A portion of such antibodies is conjugated to a labeling agent which displays a visible color change upon reaction of the antibodies with their antigenic binding partner and embedded in a first zone of an immunochromatographic assay device. Another portion of such antibodies is bound to the reaction zone of the device which has a view window. When a liquid sample, such as patient urine, cerebrospinal fluid or blood is applied to the first zone, the conjugate of antibodies and labeling agent and the sample move along a flow strip of bibulous material to the reaction ...

Reader Devices for Optical and Electrochemical Test Devices

This invention relates generally to devices and methods for performing optical and electrochemical assays and, more particularly, to devices having optical and electrochemical detectors and to methods of performing optical and electrochemical assays using such devices. The present invention is particularly useful for performing immunoassays and/or electrochemical assays at the point-of-care.

Two dimensional material based paper microfluidic device to detect and predict analyte concentrations in medical and non-medical applications

The two-dimensional material (such as graphene, hBN) based Paper Microfluidic Device will detect various analytes, which can be related to disease conditions, to minimize guesswork for a common individual to recognize a certain analyte, and faster confirmation of analytes for professionals. It will enable to predict an increase of analyte concentrations through machine learning, be applicable in both medical and non-medical fields, have a refill enclosure, and a GPS activated app to contact nearby critical infrastructure in cases of medical applications. This will have the ability to detect analytes far faster than any traditional analyte detection systems in both medical and non-medical fields.

MOLECULAR DIAGNOSTIC ASSAY DEVICE AND METHOD OF USE

Lateral flow devices and methods of use for a molecular diagnostic assay are provided. The method is suitable for detection or monitoring of targets, including biological, chemical, and material targets that exist in very low concentrations in biological samples. The methods and devices of the present application are amenable to power source-free point of care testing. 1. A method of detecting an analyte in a test sample , the method comprising; (a) a sample loading zone located upstream of a detection zone;', '(b) a reporting carrier zone located between the sample loading zone and a detection zone, wherein said reporting carrier zone comprises a reporting carrier capable of forming a complex with the analyte said reporting carrier comprising a carrier and one or more proficient enzyme cassettes; and', '(c) a detection zone, wherein the detection zone comprises a capture component for the analyte and an indicator;, '1) providing a lateral flow assay device that comprises a chromatographic medium that includesii) Contacting the sample application zone with the test sample, wherein the test sample travels through the reporting carrier zone along the chromatographic medium from the sample loading zone to the detection zone and beyond the detection zone;iii) adding a substrate to the detection zone wherein the substrate undergoes a reaction in the presence of proficient enzyme analyte containing reporting carrier; andiv) generating a response of the indicator within the detection zone that corresponds to the presence or absence of the analyte in the test sample.2. The method of claim 1 , wherein the lateral flow device further comprises a control zone down stream of the detection zone and the control zone comprises a capture component for the reporting carrier and an indicator;adding a substrate to the control zone wherein the substrate undergoes a reaction in the presence of proficient enzyme containing reporting carrier; andgenerating a response of the indicator ...

METHODS AND KITS FOR ANALYZING AUTOMOTIVE FLUIDS

The invention provides kits and methods for assessing an automotive fluid and for determining if or when an automotive fluid should be or is in need of being replaced. The kit contains a test medium that may be a paper separated into two or more distinct, separable sections or compartments containing thereon an effective amount of at least one chemical or substance that effect or facilitate a color change in the presence of one or more substance, metal or impurity such as, for instance, iron, copper, or nickel. The test medium may be arranged in a substantially cubical form and contain multiple layers of test medium.

POINT OF CARE ANALYTICAL PROCESSING SYSTEM

A point of care testing system includes a reader having an incubator disposed within a reader housing, the incubator having a rotor supported for rotation and having a plurality of circumferentially disposed slots. A drive mechanism is configured to rotate the rotor about a center axis A plurality of analytical test elements are sized for fitting in the slots of the incubator either manually or on demand. Each analytical test element commonly includes a support within a cartridge. The support retains at least one of a dry chemistry chip comprising a porous spreading layer disposed in stacked relation with at least one reagent layer or a lateral flow assay device wherein the plurality of test elements can assume a common form factor with multiplexed capability, and in which cartridges are preferably gated to enable random access processing. 1. An analytical test element comprising:a support; anda cartridge configured for retaining the support, the cartridge including an upper cover portion and a lower cover portion and in which the support retains at least one dry chemistry chip comprising a porous spreading layer disposed in stacked relation with at least one reagent layer.2. The analytical test element according to claim 1 , wherein a plurality of dry chemistry chips are disposed on the support.3. The analytical test element according to claim 1 , wherein the support retains a lateral flow assay device.4. The analytical test element according to claim 1 , wherein the cartridge includes at least one port configured for applying a quantity of sample to a sample receiving zone of the support.5. The analytical test element according to claim 3 , wherein the cartridge includes at least one port aligned with a sample receiving zone of the support and in which the sample receiving zone is common to each of the lateral flow assay device and the at least one dry chemistry chip.6. The analytical test element according to claim 5 , including a separation filter disposed ...

Method of Chemical Sensing Using a Multi-Dimensional Cross-Reactive Array

The discrimination ability of a chemical sensing cross-reactive arrays is enhanced by constructing sensing elements in two dimensions, first in the x-y plane of the substrate, second in the z dimension so that the sensors are vertically stacked on top of one another. Stacking sensing elements on top of one another adds to the discrimination ability by enabling the characteristic measurement of how fast target chemicals are passing through the stack of sensors. The new invention also allows the ability to discriminate components in a sample mixture by separating them using their innate difference in diffusional rates. Multi-sensor response patterns at each z level of sensors and time delay information from the sample passing from one level to the next are used to generate the response vector. The response vector is used to identify individual component samples and components in a mixture sample. 1. A multi-dimensional cross-reactive array for chemical sensing , comprising:a sensor substrate having stacks of sensor elements, wherein the sensor elements of a given stack are sequentially stacked such that each stack of sensor elements rises from a respective stack area as a unique sequence of sensor layers perpendicular to the sensor substrate; andan enclosure enclosing the sensor substrate such that a carrier medium carrying a chemical sample can flow across the sequentially laid out stack areas on the sensor substrate from one open end to another open end of the enclosure.2. The multi-dimensional cross-reactive array as recited in claim 1 , wherein a sensor element in a stack is comprised of a composite mix of fluorescent nanocrystals of a respective nanocrystal size and a non-fluorescent polymer chosen from Poly(vinyl stearate) claim 1 , Poly(benzyl methacrylate) claim 1 , Poly(methyl methacrylate) and Poly(ethylene-co-vinyl acetate).3. The multi-dimensional cross-reactive array as recited in claim 1 , wherein a sensor element is comprised of CdSe nanocrystals chosen ...

Quantification Method for Ammonia, Quantification Reagent, Quantification Reagent Kit, Test Piece, and Ammonia Quantification Device

A method of quantifying ammonia, the method includes performing a first reaction in which a test liquid containing ammonia is reacted with adenosine triphosphate and L-glutamic acid in the presence of glutamine synthetase to produce phosphoric acid, performing a second reaction in which the produced phosphoric acid is reacted with pyruvic acid in the presence of pyruvate oxidase, and measuring a component consumed or produced by the second reaction, to quantify ammonia, wherein a reaction to produce adenosine triphosphate from adenosine diphosphate mediated by pyruvate kinase is not carried out.

Reader Devices for Optical and Electrochemical Test Devices

This invention relates generally to devices and methods for performing optical and electrochemical assays and, more particularly, to devices having optical and electrochemical detectors and to methods of performing optical and electrochemical assays using such devices. The present invention is particularly useful for performing immunoassays and/or electrochemical assays at the point-of-care. 1. An instrument comprising:a housing including a cartridge receiving port configured to receive a plurality of testing cartridges, wherein said plurality of testing cartridges comprises at least two selected from the group consisting of: a qualitative or semi-quantitative lateral flow test device; a quantitative non-lateral flow test device; and a combined qualitative or semi-quantitative lateral flow test device and a quantitative non-lateral flow test device;an optical sensor within said port configured to read a first signal from an optical feature of said qualitative or semi-quantitative lateral flow test device or said combined qualitative or semi-quantitative lateral flow test device and a quantitative non-lateral flow test device; andan electrical connector within said port configured to mate with one or more electrodes of said quantitative non-lateral flow test device or said combined qualitative or semi-quantitative lateral flow test device and a quantitative non-lateral flow test device.2. The instrument of claim 1 , wherein said qualitative or semi-quantitative lateral flow test device or said combined qualitative or semi-quantitative lateral flow test device and said quantitative non-lateral flow test device comprises a testing system operable to detect an analyte selected from the group consisting of: hCG and drugs of abuse.3. The instrument of claim 1 , wherein said quantitative non-lateral flow test device or said combined qualitative or semi-quantitative lateral flow test device and said quantitative non-lateral flow test device comprises a testing system ...

METHOD FOR MANUFACTURING A MICROBIAL DETECTION DEVICE, MICROBIAL DETECTION METHOD, MICROBIAL DETECTION KIT, AND MICROBIAL DETECTION DEVICE

The present invention provides a method for manufacturing a microbial detection device, microbial detection method, microbial detection kit and microbial detection device. The manufacturing method includes following steps: defining a sampling portion and a reaction portion on a substrate. Fiber materials are disposed in the reaction portion and a surface of the reaction portion which contacts with the fiber materials comprises abundant hydroxyl groups. Reaction reagents are then added into the fiber materials. An acidic solution is applied to treat the fiber materials and the hydroxyl groups in the reaction portion. The present invention is advantageous for easy operation, safety, and rapid analysis. 1. A method for manufacturing a microbial detection device , including steps of:defining a sampling zone and a reacting zone on a substrate;disposing a fiber material in the reacting zone wherein the surface of the reacting zone which contacts with the fiber material comprises abundant hydroxyl groups;adding a reacting reagent onto the fiber material; andapplying an acidic solution to treat the fiber material and the hydroxyl groups.2. The method as claimed in claim 1 , wherein the reacting reagent comprises at least one selected from a group consisting of 5-methylphenazinium methosulfate and diaphorase; and at least one selected from a group consisting of 3-(4 claim 1 ,5-Dimethylthiazol-2-yl)-2 claim 1 ,5-diphenyltetrazolium bromide (MTT) claim 1 , (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchloride (INT) claim 1 , water-soluble tetrazolium salts (WSTs) claim 1 , and (2 claim 1 ,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT).3. The method as claimed in claim 1 , wherein the fiber material is α-cellulose particles.4. The method as claimed in claim 1 , wherein the acidic solution contacts the surface of the reacting zone to treat those hydroxyl groups after the acidic solution penetrates through the fiber material.5. The ...

PAPER MICROFLUIDIC DEVICES FOR FORENSIC SEROLOGY

Paper microfluidic devices for the detection of bodily fluids are provided. Such devices can be used, for example, for detection of bodily fluids from or at crime scenes, including blood, saliva, semen, urine, feces, vaginal fluids, and perspiration. Detection can be performed using colorimetric reagents that react when placed in contact with the fluid of interest. A single device can be used to test for multiple bodily fluids at the same time. 1. A microfluidic device comprising:a substrate;one or more channels on the substrate;one or more test spots on the channels, respectively; andone or more test reagents in each test spot;the one or more test reagents being suitable for testing for bodily fluids.2. The microfluidic device according to claim 1 , comprising two or more channels and two or more test spots on the substrate.3. The microfluidic device according to claim 2 , wherein the one or more channels are hydrophobic.4. The microfluidic device according to claim 1 , wherein the substrate is a paper substrate.5. The microfluidic device according to claim 2 , including a first channel claim 2 , having a first test spot and a first test reagent claim 2 , wherein the first test reagent is suitable for testing for blood.6. The microfluidic device according to claim 5 , including a second channel claim 5 , having a second test spot and a second test reagent claim 5 , wherein the second test reagent is suitable for testing for semen.7. The microfluidic device according to claim 2 , further comprising a central stem that feeds into the two or more channels.8. The microfluidic device according to claim 7 , further comprising wax on a surface of the substrate.9. The microfluidic device according to claim 7 , further comprising wax baked into or impregnated into the substrate.10. The microfluidic device according to claim 7 , further comprising a tab at an end of the central stem claim 7 , wherein the tab is suitable for receiving a test sample.11. The microfluidic device ...

Measuring Chamber, Working Method of Measuring Chamber, Chemiluminescence Measurement Method of Measuring Chamber and Chemiluminescence Detector

The present disclosure relates to a measuring chamber, a working method of the measuring chamber, a chemiluminescence measurement method of the measuring chamber and a chemiluminescence detector. The measuring chamber includes a dark chamber, a first substrate nozzle, a photomultiplier detection component, a waste liquor adsorption needle component, a reaction cup turntable and a plurality of reaction cup processing stations; the reaction cup turntable is provided in the measuring chamber rotationally; and the plurality of reaction cup processing stations are sealed in a mutually light-isolated manner. When the instrument works, reaction cups in the reaction cup turntable are moved in the dark chamber; and after the reaction cups are moved to corresponding processing stations for processing the reaction cups, the plurality of different processing stations for processing, the reaction cups may simultaneously process the reaction cups moved to the corresponding reaction cup processing stations. 1. A measuring chamber for processing multiple reaction cup processing stations in parallel , comprising a dark chamber , a first substrate nozzle , a photomultiplier detection component , a waste liquor adsorption needle component , a reaction cup turntable and a plurality of reaction cup processing stations , the reaction cup turntable being provided in the measuring chamber rotationally , wherein the plurality of reaction cup processing stations are sealed in a mutually light-isolated manner ,2. The measuring chamber as claimed in claim 1 , further comprising a bottom plate claim 1 , an inner shell claim 1 , an outer shell and an upper cover claim 1 , wherein the bottom plate claim 1 , the outer shell and the upper cover are enclosed into a holding space; the reaction cup turntable and the inner shell are provided in the holding space; the reaction cup turntable is provided between the inner shell and the outer shell along a radial direction of the reaction cup turntable; ...

OPTICAL SENSOR

An optical sensor includes an input part, a fixing part, and a determining part. The input part is provided on the upper side of the sensor. The fixing part on which a carrier is disposed is provided below the input part. The carrier has an acceptor that reacts with an analyte contained in the sample and is fixed on the carrier. The determining part includes a first metal layer, a second metal layer, and a hollow area. The first metal layer is configured to receive an electromagnetic wave. The second metal layer faces the first metal layer. The hollow area is sandwiched between the first metal layer and the second metal layer. The input part, the fixing part, and the hollow area form a part of a passage where the sample flows from the input part to the hollow area. 1. An optical sensor comprising:an input part provided on an upper side of the sensor and configured to accept a sample into the sensor;a fixing part provided below the input part and configured to dispose a carrier thereon, the carrier having an acceptor that reacts with an analyte contained in the sample and is fixed on a surface of the carrier; and a first metal layer configured to receive an electromagnetic wave;', 'a second metal layer facing the first metal layer; and', 'a hollow area sandwiched between the first metal layer and the second metal layer,, 'a determining part configured to determine a presence of the analyte in the sample, the determining part includingwherein the input part, the fixing part, and the hollow area form a part of a passage where the sample flows from the input part to the hollow area.2. The optical sensor according to claim 1 ,wherein the fixing part is capable of physically adsorbing the carrier.3. The optical sensor according to claim 1 ,wherein the input part is provided vertically above the fixing part.4. The optical sensor according to claim 1 , further comprising a discharge area connected to the hollow area of the determining part claim 1 , and positioned ...

Palette-based systems for analyte characterization

Systems, devices, and methods are provided that facilitate synthesis and analysis of chemical compounds on a convenient and automatable palette-based platform. Palettes utilized for analysis support a variety of functional sites, including test wells, separation devices, and test sites that have rapid thermal equilibration, on a single test fixture. This permits both the performance of a wide variety of tests and of tests with different temperature requirements or responses in parallel, on the same test fixture. Such systems, devices, and methods are particularly suitable for production and characterization of radiopharmaceuticals.

Analyte detection devices and systems

An analyte detection device includes a fluid impermeable layer having a first thickness and at least one inlet port having a diameter, the at least one inlet port defining a fluid pathway through the first thickness. The device also includes a reagent-hosting layer having a second thickness and including at least one of a chemical reagent and a bio-chemical reagent. The reagent-hosting layer is configured to radially receive a sampling fluid via the fluid pathway, where the sampling fluid is configured to interact with the at least one of a chemical reagent and a bio-chemical reagent in the reagent-hosting layer to indicate a characteristic associated with an analyte in the sampling fluid.

PPM QUANTIFICATION OF IODATE USING PAPER DEVICE

A highly sensitive method that utilizes a Paper Analytical Device (PAD) which measures part per million (ppm) levels of iodate iodometric titration is provided. The PAD quantifies concentrations of 0.6-15 parts per million (ppm) of iodine. The PAD has at least 12 reaction zones that contain dried reagents thereon and at least one electronically readable information zone. Salt and water are mixed and drops are placed onto 12 reaction zones that contain the loaded dry reagents. The PAD is shaken and the reaction zones turn blue if a preset iodate concentration has been exceeded. Test results are analyzed by comparing the PAD or an image of the PAD with standards, either by eye or with image analysis software to measure the color intensities which are compared to a calibration curve to quantify the iodate levels. The image analysis quantifies iodine with an average absolute accuracy and precision of 0.9 ppm over a range of 0.6-15 ppm. 1. A paper analytical device for detection of part per million levels of iodide and quantification of part per million levels of iodate by iodometric titration comprising:a porous hydrophilic medium;a plurality of reaction zones associated with the porous hydrophilic medium;at least one assay reagent which has been deposited and dried onto each of the at least reaction zones;at least one electronically readable information zone which provides a color information for the detection and quantitation of part per million levels of iodate in fortified salt when a predetermined solution of the salt has been applied onto the reaction zones,wherein the at least one electronically readable information zone comprises a plurality of fiducial markers for correct alignment of the paper analytical device for transforming and correcting a captured image such that analysis and processing of the color information to more accurately quantitate part per million levels of iodate is efficiently afforded.2. The paper analytical device of claim 1 , wherein the ...

Devices and Formulations for Detecting, Screening and Monitoring Levels of Certain Constituents in Bodily Fluids and Method

A device is disclosed for conducting a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid. An indicator formulation of the device changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A carrier substrate of the device is constructed of a material having voids providing a high void volume within the substrate. The device is made by applying a chromagen to the carrier substrate to create a chromagen-laden carrier member. Then, a selected reagent having a particular constituent-specific formulation is applied to the chromagen-laden member. The selected reagent then combines with the chromagen thereby establishing the indicator formulation within the carrier substrate in place for reception of a sample of the bodily fluid.

Testosterone Saliva Test

A lateral flow assay format test that accepts a saliva sample that is reacted with a conjugate to indicate the level of testosterone present in the saliva sample. The concentration of testosterone is used to determine the likelihood of the saliva donors commitment to an interpersonal relationship. 1. A human relationship commitment test comprised of:a lateral flow assay test integrated into a test body;the test body is adapted to accept a saliva sample;the test body has adhered to a test area a testosterone reactive conjugate;the test body has a control visual indicator that when reacted with the saliva sample proves visually that the testosterone reactive conjugate is reacting effectively with testosterone in the saliva sample;the test body has a test visual indicator that when reacted with the saliva sample visually indicates the concentration of testosterone in the saliva sample by varying intensity of the test visual indicator;if the intensity of the test visual indicator is at or below a predetermined threshold then human relationship commitment is determined lacking.2. A human relationship commitment test as in further characterized in that an electronic sensor is paired with the test body that optically determines an intensity of the test visual indicator relative to an intensity of the control visual indicator then calculates and displays in a human readable format a quantified concentration of testosterone in the saliva sample. This application claims benefit to provisional patent application 62/430,432 titled “Testosterone Saliva Test” filed on 6 Dec. 2016 that is incorporated herein in its entirety by reference.The present invention relates to human medical testing, and more particularly, to a non-invasive test to determine testosterone levels of men as part of a determination of their fidelity to interpersonal romantic relationships.Several designs for testosterone testing in humans have been designed in the past. None of them, however, includes a ...

MOLECULAR DIAGNOSTIC ASSAY DEVICE AND METHOD OF USE

Lateral flow devices and methods of use for a molecular diagnostic assay are provided. The method is suitable for detection or monitoring of targets, including biological, chemical, and material targets that exist in very low concentrations in biological samples. The methods and devices of the present application are amenable to power source-free point of care testing. 1. A method of detecting an analyte in a test sample , the method comprising; (a) a sample loading zone located upstream of a detection zone;', '(b) a reporting carrier zone located between the sample loading zone and a detection zone, wherein said reporting carrier zone comprises a reporting carrier capable of forming a complex with the analyte said reporting carrier comprising a carrier and one or more proficient enzyme cassettes; and', '(c) a detection zone, wherein the detection zone comprises a capture component for the analyte and an indicator;, 'i) providing a lateral flow assay device that comprises a chromatographic medium that includesii) Contacting the sample application zone with the test sample, wherein the test sample travels through the reporting carrier zone along the chromatographic medium from the sample loading zone to the detection zone and beyond the detection zone;iii) adding a substrate to the detection zone wherein the substrate undergoes a reaction in the presence of proficient enzyme analyte containing reporting carrier; andiv) generating a response of the indicator within the detection zone that corresponds to the presence or absence of the analyte in the test sample.2. The method of claim 1 , wherein the lateral flow device further comprises a control zone down stream of the detection zone and the control zone comprises a capture component for the reporting carrier and an indicator;adding a substrate to the control zone wherein the substrate undergoes a reaction in the presence of proficient enzyme containing reporting carrier; andgenerating a response of the indicator ...

Screening Test Paper Reading System

The invention discloses a screening test paper reading system comprising a screening test paper, a test paper carrier, a delivery unit and a reading unit. The screening test paper is provided with a plurality of reaction zones thereon for reacting with a plurality of specific samples, and changing their own color. The test paper carrier is provided with a bearing groove thereon for accommodating the screening test paper and the test paper carrier is provided with a plurality of positioning parts and a gearing part thereon. The delivery unit has a driving device and a detection device. When the detection device detects a plurality of positioning parts on the test paper carrier, the driving device is activated and the test paper carrier is moved by the gearing part so that the reading unit retrieves a color signal on each the reaction zone, whereby the screening test paper reading system can achieve an effect of a fast screening and protection of the screening test paper. 1. A screening test paper reading system comprising:a screening test paper provided with a plurality of reaction zones thereon for reacting with a plurality of specific samples, and changing their own color;a test paper carrier provided with a bearing groove thereon for accommodating the screening test paper and the test paper carrier provided with a plurality of positioning parts and a gearing part thereon;a delivery unit having a driving device and a detection device; anda reading unit,wherein when the detection device detects the positioning parts on the test paper carrier, the driving device is activated and the test paper carrier is moved by the gearing part so that the reading unit retrieves a color signal on each the reaction zone.2. The screening test paper reading system according to claim 1 , wherein the delivery unit further comprises a caging device for limiting a position of the test paper carrier when it is moved.3. The screening test paper reading system according to claim 1 , wherein ...

Methods for detecting target dna sequences

The invention relates to methods for detecting target DNA sequences, particularly for detecting Leishmania infection, comprising a recombinase-polymerase isothermal amplification and detection by electrochemistry. The invention also relates to kits and oligonucleotides for performing said methods.

INTELLIGENT TEST STRIP DETECTION DEVICE AND METHOD THEREOF

Provided are an intelligent test strip detection device and method thereof. The intelligent test strip detection device includes a measuring unit, having a test strip end and a test strip reacting zone, wherein the test strip end tests a liquid with at least one kind of hormones, and the test strip reacting zone renders a result of the liquid under test; and a reading unit, having a slot, a signal processing circuit, a driver, a timing generator, a plurality of light sources, a plurality of photo sensors, and a connecting end, wherein the signal processing circuit is electrically connected to the driver, the timing generator, the plurality of light sources, and the plurality of photo sensors. Moreover, the measuring unit is inserted into the slot of the reading unit, and the connecting end of the reading unit is connected to a contact hole of a portable electronic device. 1. An intelligent test strip detection device , comprising:a measuring unit, including a test strip end and a test strip reaction zone, wherein the test strip end measures a test solution containing at least one physiological hormone, and the test strip reaction zone is used for reacting with the test solution so as to display a detected result; anda reading unit, including a slot, a signal processing circuit, a driver, a timing generator, a plurality of light sources, a plurality of light sensors and a connecting end, wherein the signal processing circuit is electrically connected to the driver, the timing generator, the plurality of light sources and the plurality of light sensors;wherein the measuring unit is inserted into the slot of the reading unit, the connecting end of the reading unit is connected to a connecting hole of the portable electronic device.2. The intelligent test strip detection device of claim 1 , wherein the test solution is urine from a human body claim 1 , and comprising a follicle-stimulating hormone (FSH) and a luteinizing hormone (LH).3. The intelligent test strip ...

TEST INSTRUMENT AND METHOD OF CONTROLLING THE SAME

Disclosed herein are a test instrument and a method of controlling the same, capable of performing a basic test on a sample of a patient, determining whether to perform an additional test, and displaying interfaces associated with a progress level, a necessary time, etc. of the additional test on a display unit when the additional test is performed, thereby enabling a user to visibly recognize information associated with a performing process of each test. The test instrument includes: a detection unit configured to apply light to at least one chamber in which a reaction between a reagent and a sample occurs and to detect an optical signal from the chamber to test the sample held in a reaction device, a control unit configured to determine whether to perform a secondary test on the sample after a primary test is performed on the sample and to control to display a secondary test progress interface showing a progress level of the secondary test when the secondary test is performed, and a display unit configured to display a primary test progress interface showing a progress level of the primary test on the sample and to display the secondary test progress interface showing the progress level of the secondary test when the secondary test is performed on the sample. 1. A test instrument comprising:a detection unit configured to apply light to at least one chamber in which a reaction between a reagent and a sample occurs and to detect an optical signal from the chamber to test the sample held in a reaction device;a control unit configured to determine whether to perform a secondary test on the sample after a primary test is performed on the sample and to control to display a secondary test progress interface showing a progress level of the secondary test when the secondary test is performed; anda display unit configured to display a primary test progress interface showing a progress level of the primary test on the sample and to display the secondary test progress ...

Food Allergen Detection Methods and Systems Using Molecularly Imprinted Polymers

Methods and devices for the detection of food allergens using molecularly imprinted polymers that are imprinted for a target food allergen. A molecularly imprinted polymer may be imprinted using surface imprinting or other procedures. Detection of food allergens, such as peanut allergens, may be accomplished using all or a portion of a protein food allergen as a template to produce a molecularly imprinted polymer for food allergen detection. A portion utilized can be one that creates receptor sites in the molecularly imprinted polymer that are unique or more unique to the target food allergen than receptor sites that would be created if an entire food allergen molecule were utilized.

ANALYTE DETECTION DEVICES AND METHODS WITH HEMATOCRIT/VOLUME CORRECTION AND FEEDBACK CONTROL

Disclosed are devices, arrangements and methods for quantifying the concentration of an analyte present in bodily fluid, including: an assay pad having at least one chemical reagent capable of producing a detectable signal in the form of a reaction spot upon reaction with the analyte; a light source; a detector array; a processor; and a memory in communication with the processor, the memory comprising: (a) at least one value indicative of one or more of: (i) the level of hematocrit contained in the sample; (ii) the volume of the sample applied to the assay pad; or (iii) imperfections present in the reaction spot; and (b) at least one algorithm for calculating the concentration of the analyte contained in the sample. 127-. (canceled)28. A method of collecting a body fluid sample using an analyte meter comprising an assay pad and a detector , the method comprising:applying a catalyst to a sampling site of a user to facilitate acquisition of the body fluid sample;imaging the assay pad using the detector;determining whether the assay pad has received a sufficient sample volume from the sampling site; andaltering or maintaining the application of the catalyst to the sampling site based on the determination.29. The method of claim 28 , wherein the catalyst is vacuum claim 28 , heat claim 28 , pressure claim 28 , or vibration.30. The method of claim 28 , wherein altering or maintaining the application of the catalyst comprises stopping the application of the catalyst.31. The method of claim 28 , wherein altering or maintaining the application of the catalyst comprises increasing or decreasing a magnitude of the catalyst.32. The method of claim 28 , wherein the detector comprises a detector array.33. The method of claim 32 , wherein the detector array comprises a linear array of detector elements claim 32 , and the detector elements comprise CMOS claim 32 , CCD claim 32 , photodiode claim 32 , infrared claim 32 , fluorescent claim 32 , ultraviolet or electrochemical ...

DETECTION METHOD, DEVICE AND CARTRIDGE FOR ENHANCING DETECTION SIGNAL INTENSITY

A detection method for enhancing detection signal intensity is provided. The detection method includes the following steps. Firstly, a detection device is provided. The detection device includes a channel, an inlet port and an air chamber. The air chamber includes an elastic layer. A bonding material is immobilized in the channel and served as a reaction area. Then, a sample containing a detection material is loaded into the inlet port. As the elastic layer is moved upwardly and downwardly, the sample is moved toward the air chamber and the inlet port in a reciprocating manner. Consequently, the possibility of combining the detection material of the sample with the bonding material in the reaction area is increased. Afterwards, an optical signal from the reaction area is measured. 1. A detection method for enhancing detection signal intensity , the detection method comprising steps of:(a) providing a detection device, wherein the detection device comprises a channel, an inlet port and an air chamber, wherein the inlet port is in communication with a first end of the channel, the air chamber is in communication with a second end of the channel, the air chamber comprises an elastic layer, and a bonding material is immobilized in the channel and served as a reaction area;(b) loading a sample into the inlet port, wherein the sample contains a detection material;(c) moving the elastic layer upwardly to transfer the sample in a direction toward the second end of the channel, so that a portion of the detection material is combined with the bonding material;(d) moving the elastic layer downwardly to transfer the sample in a direction toward the first end of the channel, so that another portion of the detection material is combined with the bonding material;(e) repeatedly performing the step (c) and the step (d) for at least one time;(f) moving the elastic layer downwardly to remove the sample from the reaction area; and(g) measuring an optical signal from the reaction area. ...

Colorimetric analyzer with de-bubbling

A colorimetric analyzer includes a reaction chamber configured to receive a sample and at least one reagent. A measurement cell is operably coupled to the reaction chamber. The measurement cell has an illumination source and an illumination detector spaced from the illumination source such that illumination from the illumination source passes through the reacted sample to the illumination detector. A controller is coupled to the illumination source and the illumination detector. The controller is configured to generate an analytic output based on a signal from the illumination detector. A fill conduit is operably interposed between the reaction chamber and the measurement cell. The fill conduit is configured to reduce bubbles.

Assay test device, kit and method of using

The present invention relates to assay test devices, and methods and kits for use to monitor, sense, read and display results by using devices with printed electronics, such as batteries, reading devices, and other circuitry and/or using colorimetric means for testing by using a sensitive indicator pH dye, or both.

Multi-Layered Devices for Analyte Detection

A multi-layered device for detecting the presence or absence of an analyte within a test sample is described. The device includes a sensing layer and a control layer. The sensing layer is configured to support a reaction so as to exhibit a signal indicative of the presence or absence of the analyte in the test sample. The control layer is in fluid communication with and vertically adjacent to the sensing layer and includes a reagent capable of inhibiting the reaction and/or other unwanted side reactions at the sensing layer after a certain period of time by diffusive movement of the reagent from the control layer to the sensing layer. 15-. (canceled)6. A multi-layered device for detecting the presence or absence of an enzyme within a test sample , the device comprising:a sensing layer, the sensing layer comprising a substrate capable of being modified in the presence of the enzyme to release a product utilized to indicate the presence or absence of the enzyme in the test sample; anda control layer, the control layer being in fluid communication with and vertically adjacent to the sensing layer, the control layer including a control reagent capable of inhibiting the reaction at the sensing layer by diffusive movement of the control reagent from the control layer to the sensing layer.7. The multi-layered device of claim 6 , wherein the enzyme is an esterase.8. The multi-layered device of claim 6 , wherein the substrate is an aromatic ester.9. The multi-layered device of claim 6 , wherein the product comprises a nucleophilic aromatic compound.10. The multi-layered device of claim 9 , wherein the nucleophilic aromatic compound comprises benzopyrrole.11. The multi-layered device of claim 6 , wherein the sensing layer further comprises a diazonium ion.12. The multi-layered device of claim 6 , wherein the control layer is positioned underneath the sensing layer.13. The multi-layered device of claim 6 , wherein the sensing layer is covered by a material defining an opening ...

Device for use in the detection of binding affinities

A device for use in the detection of binding affinities, the device comprising a planar waveguide ( 2 ) arranged on a substrate ( 3 ), and further comprising an optical coupler ( 41 ) having a predetermined length for coupling coherent light ( 1 ) of a predetermined wavelength into the planar waveguide ( 2 ) such that a parallel beam of coherent light propagates through the planar waveguide ( 2 ) with an evanescent field ( 11 ) of the coherent light propagating along an outer surface ( 21 ) of the planar waveguide ( 2 ). The outer surface ( 21 ) of the planar waveguide ( 2 ) comprises binding sites thereon capable of binding target samples to the binding sites such that light of the evanescent field ( 11 ) is diffracted by target samples bound to the binding sites. The binding sites are arranged along a plurality of predetermined straight lines ( 7 ) running parallel to one another with a constant distance between adjacent straight lines. The predetermined straight lines ( 7 ) are arranged at an angle relative to the direction of propagation of the evanescent field ( 11 ) such that the coherent light ( 12 ) diffracted by the target samples bound to the binding sites impinges under a diffraction angle relative to the straight lines onto a further optical coupler ( 8 ) arranged in a portion ( 10 ) of the planar waveguide ( 2 ) outside the beam of coherent light propagating through the planar waveguide. The further optical coupler ( 8 ) couples the diffracted coherent light ( 13 ) out of the planar waveguide ( 2 ) such as to interfere at a predetermined detection location ( 9 ) with a difference in optical path length which is an integer multiple of the predetermined wavelength.

DEFINITIVE DEVELOPMENT DIAGNOSTIC ANALYSIS

Detecting a definitive presence or absence of an analyte from a borderline test is shown and described. In one embodiment, a method of generating a definitive test result includes continuously incubating the assay concurrently with continuously reading a diagnostic test on the assay. In particular examples, reading the diagnostic test on the assay includes performing a one minute diagnostic reading. Typically, when a borderline test result is detected, further development of the assay is performed. In most examples, detecting a definitive presence or absence test result deactivates the testing sequence. 1. A method of generating a definitive test result from an assay for detecting the presence or absence of an analyte , the method comprising:a. incubating said assay in an incubation environment;b. reading a diagnostic test on said assay concurrently as an incubator incubates said assay; andc. performing continuous reading of said diagnostic test and incubating of said assay of a borderline test result to generate said definitive test result.2. The method of claim 1 , wherein reading said diagnostic test includes performing a one minute diagnostic reading.3. The method of claim 1 , wherein generating a definitive test result includes reading a predetermined difference between a reflectance value on a control line and a reflectance value a test line.4. The method of claim 1 , wherein generating a definitive test result includes reading a predetermined difference between a reflectance value of a control line and a reflectance value of test line claim 1 , and a predetermined reflectance value on said control line.5. The method of claim 1 , wherein detecting said definitive positive test includes deactivating said system.6. The method of claim 1 , wherein detecting a definitive negative test includes deactivating said system.7. The method of claim 1 , including monitoring a pre-test analysis on said assay.8. The method of claim 1 , including decoding a reference coding ...

FAST FLOW APPARATUS AND METHOD TO EVALUATE ANALTYES IN LIQUID, SOLID AND SEMISOLID SAMPLES

Disclosed is a fast flow device, for detecting an analyte in a sample, comprising a grooved sample pad for collecting the sample, a test strip coupled to the sample pad, and a housing enclosing and allowing visualization of the test strip. The sample pad can include markings for determining the amount of sample collected. The device can also preferably include a vented flexible collar connecting the housing to a run fluid container, which vented flexible collar fits over the sample pad and forms an air-tight seal between the housing and the run fluid container. The device achieves fast flow because the venting equalizes internal pressure of the housing and run fluid container and because of the grooving on the sample pad. Further, the device can include a second sample pad/test strip combination for detection of a second analyte. Also disclosed are methods for detecting an analyte in a sample using the device. 1. A fast flow device for performing a test reaction on a sample to detect an analyte comprising:a grooved sample pad for collecting the sample, the sample pad comprising at least one marking providing a visual indication of the amount of sample collected, wherein the at least one marking is disposed and located on the sample pad so as to provide visual measurement indication, in both the presence or absence of sample, of a minimum or maximum amount of sample to be collected for delivering sample to the test strip for determining a presence or absence of an analyte in the sample, the sample pad having a backside embossed with at least one groove to fast flow fluid;a test strip coupled to the sample pad, the test strip comprising an indicator region configured to provide a visual indication of the presence or absence of the analyte in the sample; anda housing comprising a first end and a second end, wherein the test strip is disposed within the housing, the housing allowing visualization of the test strip, and wherein at least a portion of the sample pad ...

Testing Device For Identifying Antigens And Antibodies In Biofluids

A testing device for identifying an antigen or antibody within a biofluid sample including: a substrate having a hydrophilic surface thereon; the surface including a collection zone, and at least one detection zone extending therefrom; wherein the biofluid sample can be mixed with a specific antigen or antibody, and deposited on the collection zone and transferred by capillary action to the detection zone; the antigen or antibody in the biofluid sample reacting with an appropriate said antibody or antigen thereby resulting in a visual indication within the detection zone. 1. A method for identifying an antigen or antibody within a blood sample using a testing device having a cellulosic material substrate having a hydrophilic surface thereon , the surface including a collection zone and at least one detection zone extending therefrom , the detection zone having an antibody or antigen immobilized therein , the method including the steps of:depositing the blood sample on the collection zone of the testing device;the blood sample being transferred by capillary action to the detection zone allowing the blood sample to react for a predefined optimal time; andidentifying the antigen or antibody by a resultant visual indication arising where the antibody or antigen in the blood sample reacts with an appropriate said antigen or antibody within the detection zone, wherein the resultant visual indication results from reduced wicking and/or a differentiation of eluation speeds.2. A method according to claim 1 , including mixing the blood sample with a specific antigen or antibody before depositing the blood sample on the collection zone of the testing device.3. A method according to claim 1 , including mixing nanoparticles within the blood sample to facilitate said reaction in the detection zone.4. A method according to claim 1 , wherein the visual indication is due to an agglutination of the blood upon reaction with a specific antibody resulting in reduced wicking of the blood ...

SYSTEM FOR CHEMILUMINESCENCE-BASED DETECTION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS

The present disclosure comprises a device and accompanying method for determining the presence or absence of Methicillin-resistant in a sample. The disclosure includes the following elements: () a lateral flow strip for microfluidic manipulation of a sample; () a cassette device for containing the lateral flow strip and enabling interface with a detection device; () a cassette handler; () a luminous reagent delivery device; and () an electromagnetic radiation detection device capable of converting chemiluminescent radiation from the lateral flow strip into an output for a user. 1Staphylococcus aureus. A cassette device for determining the presence or absence of Methicillin-resistant (“MRSA”) in a sample , comprising:(a) a lateral flow strip, comprising (1) an absorbent pad for receiving a liquid sample mixed with an enzyme conjugate, (2) a region for accepting a luminous reagent, (3) a region comprising a reaction zone, wherein the reaction zone includes a substance binding region, a negative control region and a positive control region, and (4) a microfluidic pump, and wherein (i) the sample acceptor pad, the luminous reagent accepting region, the reaction zone, and microfluidic pump are in fluid communication, and facilitate fluid flow in a downstream direction from the sample acceptor pad to the microfluidic pump; (ii) the substance binding region includes an antibody for capturing a target substance; (iii) the positive control region includes an antibody for capturing the enzyme conjugate, and (iv) the enzyme conjugate is capable of binding with the substance and reacting with the luminous reagent, thereby generating electromagnetic radiation; and(b) a housing, comprising (1) an upper component comprising a first basin for sample preparation, a second basin for sample preparation, a first well for adding a sample, a second well for adding a luminous reagent, a plurality of apertures to allow electromagnetic radiation generated on the lateral flow strip to escape ...

COMPOSITIONS, APPARATUS AND METHODS FOR MONITORING AND IMPROVING ORAL HEALTH

The present disclosure relates to methods, compositions and apparatus for monitoring and improving oral health by utilizing information about nitric oxide levels in an individual's oral cavity. In an embodiment, the disclosure provides methods and apparatus for monitoring nitric oxide metabolites in saliva; this information may be utilized as it relates to improving oral health, i.e. monitoring and improving oral hygiene and increasing the consumption of nitric oxide-potent foods. In an embodiment, methods for improving oral health in a subject comprising, use of an oral cleanser by an individual, wherein the oral cleanser comprises a potassium nitrate source; and use of a saliva test strip for measuring nitrite and nitrogen oxides in the oral cavity of the individual are provided. In an embodiment, a novel nitrate-rich gum composition is provided. 1. A method for improving oral health in a subject comprising ,using a vehicle for increasing the presence of nitrate in the oral cavity of a subject;monitoring the presence of nitrate-to-nitrite bioconversion in the oral cavity of a subject using a saliva test strip for measuring nitrite in the oral cavity of the subject, andimplementing oral hygiene steps when the saliva test strip indicates detects nitrate-to-nitrite bioconversion.2. The method of claim 1 , wherein the vehicle for increasing the presence of nitrate comprises the introduction of a nitrate-based substance into the oral cavity.3. The method claim 2 , wherein the nitrate-based substance comprises a gum or a lozenge.4. The method of claim 3 , wherein the gum comprises inorganic nitrate potassium nitrate claim 3 , nitric oxide donors claim 3 , acidified nitrite claim 3 , nitrite claim 3 , organic nitrate claim 3 , sodium nitroprusside claim 3 , NONOates claim 3 , S-nitrosthiols claim 3 , or probiotic NO producing nanoparticles or substrates contained within a gum base.5. The method of claim 4 , further comprising flavorings claim 4 , binders claim 4 , ...

METHOD FOR MANUFACTURING A MICROBIAL DETECTION DEVICE, MICROBIAL DETECTION METHOD, MICROBIAL DETECTION KIT, AND MICROBIAL DETECTION DEVICE

The present invention provides a method for manufacturing a microbial detection device, microbial detection method, microbial detection kit and microbial detection device. The manufacturing method includes the following steps: defining a sampling portion and a reaction portion on a substrate. Fiber materials are disposed in the reaction portion and a surface of the reaction portion which contacts with the fiber materials comprises abundant hydroxyl groups. Reaction reagents are then added into the fiber materials. An acidic solution is applied to treat the fiber materials and the hydroxyl groups in the reaction portion. 1. A method for manufacturing a microbial detection device , including steps of:defining a sampling zone and a reacting zone on a substrate;disposing a fiber material in the reacting zone wherein the surface of the reacting zone which contacts with the fiber material comprises abundant hydroxyl groups;adding a reacting reagent onto the fiber material; andapplying an acidic solution to treat the fiber material and the hydroxyl groups.2. The method as claimed in claim 1 , wherein the reacting reagent comprises at least one selected from a group consisting of 5-methylphenazinium methosulfate and diaphorase; and at least one selected from a group consisting of 3-(4 claim 1 ,5-Dimethylthiazol-2-yl)-2 claim 1 ,5-diphenyltetrazolium bromide (MTT) claim 1 , (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchloride (INT) claim 1 , water-soluble tetrazolium salts (WSTs) claim 1 , and (2 claim 1 ,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT).3. The method as claimed in claim 1 , wherein the fiber material is α-cellulose particles.4. The method as claimed in claim 1 , wherein the acidic solution contacts the surface of the reacting zone to treat those hydroxyl groups after the acidic solution penetrates through the fiber material.5. The method as claimed in claim 1 , wherein the substrate is a fibrous substrate.6. The ...

Apparatus and method for homogeneous immunoassay

Apparatus and method for an immunoassay of a binding reaction between a ligand and an antiligand which are typically an antigen and an antibody, including a spatial pattern formed by a spatial array of separate regions of antiligand material, and ligand material dispersed to interact with the spatial array of separate regions of antiligand material for producing a binding reaction between the ligand and the antiligand in the spatial patterns and with the bound complexes labeled with a particular physical characteristic. A source of input energy and with the input energy at a particular spectrum for interacting with particular physical characteristic of the labeled binding reaction. Scanning the spatial pattern with the input energy at the particular spectrum for producing output energy having amplitude levels formed by a substantially random background component and a non-random component representing the labeled bound complexes, and the non-random component representing the labeled bound complexes detected to produce an output signal in accordance with the labeled binding reaction.

Immunoassay

Apparatus and method for providing an immunoassay of a binding reaction between a ligand and an antiligand which are typically an antigen and an antibody, including a spatial pattern formed by a spatial array of separate regions of antiligand material, and ligand material dispersed to interact with the spatial array of separate regions of antiligand material for producing a binding reaction between the ligand and the antiligand in the spatial patterns and with the bound complexes labeled with a particular physical characteristic. A source of input energy and with the input energy at a particular spectrum for interacting with particular physical characteristic of the labeled binding reaction. Scanning the spatial pattern with the input energy at the particular spectrum for producing output energy having amplitude levels formed by a substantially random background component and a non-random component representing the labeled bound complexes, and the non-random component representing the labeled bound complexes detected to produce an output signal in accordance with the labeled binding reaction.

空間パタンを用いた高感度免疫分析装置

(57)【要約】本公報は電子出願前の出願データであるた め要約のデータは記録されません。

A method for detecting optical phase changes during biosensor operation, biosensing apparatus and a biosensor adapted for use in the same

This invention discloses a biosensing apparatus comprising: a resonant optical biosensor in which a coating layer thereof is in part sensitized to at least one given assay species and is patterned for causing light diffracton; an extended light source; collimating means arranged to direct light from the extended light source onto the resonant coupled optical biosensor; a light detector; light focussing means arranged to direct light diffracted from the patterned sensitized coating onto the light detector thereby to define a diffraction field in the plane of the detector; and, scanning means arranged to scan the light detector relative to this diffraction field. This invention also discloses a method for detecting optical phase changes during biosensor operation.

Solid-phase binding assay system and method for interferometrically measuring analytes bound to an active receptor

Apparatus (10) and method for detecting and measuring analyte in biological and other sample solutions. A substrate (20) is provided with at least one active region which specifically binds analyte and an inactive region that does not, and a change in optical path length through the active region due to binding of analyte is measured. The substrate (20) is inserted into one or both beams of an interferometer (10) and moved relative to the interferometer (10). As the substrate (20) moves, the active and inactive regions pass successively through the beam(s) causing a periodic phase shift in the light. When the beams, one or both of which have undergone phase modulation, are recombined with each other, the phase modulation is converted into an amplitude modulation. Each recombined beam is directed to a photodetector (42, 45), which converts the periodically varying optical power into a periodically varying electrical signal. This signal has an amplitude proportional to the amount of bound analyte on the surface of the substrate (20) and a signal frequency equal to that at which the active regions move past the beam(s) in the interferometer (10). Servo control (60, 62, 65) is used to maintain the interferometer at a desired operating point that maximizes the sensitivity and linearity of the system so that the small phase differences due to analyte binding can be measured more accurately.

Portable devices for detection of antibodies against therapeutic drugs

Portable devices for anti-drug antibodies (ADAs) testing are provided. These devices can be used in various applications, including but not restricted to the following: uniform testing of patients for ADAs; selection of therapeutic drug for patient treatment; evaluation of the need to change therapeutic drug or to apply tolerance regimens; selection of patients for clinical trials; comparison of therapeutic drugs marketed for a given disease and also gene therapy; scientific guidance for discovering therapeutic drugs; therapeutic drug development; postmarketing surveillance of therapeutic drugs.

Solid-phase binding assay system for interferometrically measuring analytes bound to an active receptor

A solid-phase binding assay that measures interferometrically either antibodies or binding pair antigens bound to a spinning disc on which the complementary antigen or antibody has previously been coated in an alternating pattern of immunologically active and inactive spots. The spinning disc is inserted into one arm of a Mach-Zehnder interferometer and as the disc spins the bound and unbound spots pass one after another through the two light beams causing a periodic phase shift in the light. These phase modulated light beams are then recombined at a beam-splitter converting the phase modulation into an amplitude modulation. The two recombined beams then fall on a photodetector which converts the periodically varying optical power into a periodically varying electrical current whose amplitude is proportional to the amount of bound protein on the surface of the disc and whose modulation frequency is equal to the frequency with which the spots pass through the light beam. If the disc is spinning rapidly and there are many spots around the disc this signal frequency will be much higher than the frequency of the noise due to the disc wobble and the vibrations imparted to the interferometer by the spinning motor.

Device for use in the detection of binding affinities

결합 친화성의 검출에 사용하기 위한 장치로서, 기판(3) 상에 배열된 평면 도파로(2)를 포함하고, 추가로 간섭광의 평행 광선이 평면 도파로(2)의 외면을 따라 전파하는 간섭광의 소산장(11)을 이용해 평면 도파로(2)를 통해 전파하도록 평면 도파로(2) 내로 소정의 파장의 간섭광(1)을 커플링하기 위한 소정의 길이를 갖는 광커플러(41)를 포함하는 장치가 제공된다. 평면 도파로(2)의 외면(21)은, 소산장(11)의 빛이 결합 부위에 결합한 표적 시료에 의해 회절되도록 결합 부위에 표적 시료를 결합시킬 수 있는 결합 부위를 그 위에 포함한다. 상기 결합 부위는 인접한 직선들 사이에 일정 거리로 서로에 대해 평행하게 이어지는 복수의 소정의 직선들(7)을 따라서 배열된다. 소정의 직선들(7)은, 결합 부위에 결합한 표적 시료에 의해 회절된 간섭광(12)이 평면 도파로를 통해 전파하는 간섭광의 광선 외부에서 평면 도파로(2)의 부분(10)에 배열된 추가의 광커플러(8)상에서 직선들에 대한 회절각으로 충돌하도록 소산장(11)의 전파 방향에 대한 각도로 배열된다. 추가의 광커플러(8)는 소정의 파장의 정수 배수인 광학 경로 길이에의 차이를 갖는 소정의 검출 위치(9)에서 간섭하도록 평면 도파로(2)를 벗어난 회절된 간섭광(13)을 커플링한다. An apparatus for use in the detection of binding affinity, comprising a planar waveguide (2) arranged on a substrate (3), and additionally, a dissipation field of interfering light through which parallel rays of interference light propagate along the outer surface of the planar waveguide (2) Provided is an apparatus comprising an optocoupler 41 having a predetermined length for coupling interference light 1 of a predetermined wavelength into the planar waveguide 2 to propagate through the planar waveguide 2 using (11) do. The outer surface 21 of the planar waveguide 2 includes thereon a binding site capable of binding the target sample to the binding site such that light of the dissipation field 11 is diffracted by the target sample binding to the binding site. The joining sites are arranged along a plurality of predetermined straight lines 7 running parallel to each other at a certain distance between adjacent straight lines. The predetermined straight lines 7 are additionally arranged in the portion 10 of the planar waveguide 2 outside the ray of the interfering light, which is diffracted by the target sample bound to the binding site and propagates through the planar waveguide. It is arranged at an angle to the propagation direction of the dissipation field 11 so as to collide at a diffraction angle for straight lines on the ...

Device and Method for Determination of a Catalyst State in a Chemical Reactor

The invention pertains to a device for determination of a catalyst state in a chemical reactor and to a method for detecting a catalyst state under in situ reaction conditions. A reactor is provided with a solid catalyst provided in a reactor chamber. A fluid sample is taken from the reactor chamber and is transferred to a sample chamber. The temperature at the extraction site of the sample in the reactor chamber is determined and the temperature of the sample chamber is adjusted to the same temperature. A small amount of the catalyst provided in reactor chamber is provided in sample chamber and is contacted with the sample flow. Spectroscopic information is then obtained on the catalyst provided in sample cell, e.g. by an IR spectrometer. 1. A device for determination of a catalyst state in a chemical reactor , comprising:a reactor comprising a reactor chamber with at least one inlet for introducing a fluid phase into the reactor chamber and at least one outlet for draining off the fluid phase,a sampling device for collecting a fluid sample inside the reactor chamber,a temperature-sensitive sensor arranged at the sampling device for detecting a temperature of a fluid phase and/or a solid phase at the site of the sampling device where the fluid sample is collected,whereina spectroscopic detection unit arranged outside the reactor, said spectroscopic unit comprising a sample chamber for arranging a sample therein and a spectroscopic analysis device for obtaining spectroscopic data on the sample arranged in the sample chamber,a conduit is provided between sampling device and the sample chamber of the spectroscopic detection unit for introducing a sample taken at the sampling device into the sample chamber of the spectroscopic detection unit,heating and/or cooling means and a temperature control unit is provided in the spectroscopic detection unit for adjustment of a temperature of the sample chamber.2. A device according to claim 1 , wherein the sampling device is a ...

결합 친화성의 검출에 사용하기 위한 장치

결합 친화성의 검출에 사용하기 위한 장치로서, 기판(3) 상에 배열된 평면 도파로(2)를 포함하고, 추가로 간섭광의 평행 광선이 평면 도파로(2)의 외면을 따라 전파하는 간섭광의 소산장(11)을 이용해 평면 도파로(2)를 통해 전파하도록 평면 도파로(2) 내로 소정의 파장의 간섭광(1)을 커플링하기 위한 소정의 길이를 갖는 광커플러(41)를 포함하는 장치가 제공된다. 평면 도파로(2)의 외면(21)은, 소산장(11)의 빛이 결합 부위에 결합한 표적 시료에 의해 회절되도록 결합 부위에 표적 시료를 결합시킬 수 있는 결합 부위를 그 위에 포함한다. 상기 결합 부위는 인접한 직선들 사이에 일정 거리로 서로에 대해 평행하게 움직이는 복수의 소정의 직선들(7)을 따라서 배열된다. 소정의 직선들(7)은, 결합 부위에 결합한 표적 시료에 의해 회절된 간섭광(12)이 평면 도파로를 통해 전파하는 간섭광의 광선 외부에서 평면 도파로(2)의 부분(10)에 배열된 여분의 광커플러(8)상에서 직선들에 대한 회절각으로 충돌하도록 소산장(11)의 전파 방향에 대한 각도로 배열된다. 추가의 광커플러(8)는 소정의 파장의 정수 배수인 광학 경로 길이에의 차이를 갖는 소정의 검출 위치(9)에서 간섭하도록 평면 도파로(2)를 벗어난 회절된 간섭광(13)을 커플링한다.

Analyte detection devices and methods with hematocrit/volume correction and feedback control

Disclosed are devices, arrangements and methods for quantifying the concentration of an analyte present in bodily fluid, including: an assay pad having at least one chemical reagent capable of producing a detectable signal in the form of a reaction spot upon reaction with the analyte; a light source; a detector array; a processor; and a memory in communication with the processor, the memory comprising: (a) at least one value indicative of one or more of: (i) the level of hematocrit contained in the sample; (ii) the volume of the sample applied to the assay pad; or (iii) imperfections present in the reaction spot; and (b) at least one algorithm for calculating the concentration of the analyte contained in the sample.

Analysis equipment

Analyte detection devices and methods with hematocrit/volume correction and feedback control

Disclosed are devices, arrangements and methods for quantifying the concentration of an analyte present in bodily fluid, including: an assay pad having at least one chemical reagent capable of producing a detectable signal in the form of a reaction spot upon reaction with the analyte; a light source; a detector array; a processor; and a memory in communication with the processor, the memory comprising: (a) at least one value indicative of one or more of: (i) the level of hematocrit contained in the sample; (ii) the volume of the sample applied to the assay pad; or (iii) imperfections present in the reaction spot; and (b) at least one algorithm for calculating the concentration of the analyte contained in the sample.

Analyte detection devices and methods with hematocrit/volume correction and feedback control

Disclosed are devices, arrangements and methods for quantifying the concentration of an analyte present in bodily fluid, including: an assay pad having at least one chemical reagent capable of producing a detectable signal in the form of a reaction spot upon reaction with the analyte; a light source; a detector array; a processor; and a memory in communication with the processor, the memory comprising: (a) at least one value indicative of one or more of: (i) the level of hematocrit contained in the sample; (ii) the volume of the sample applied to the assay pad; or (iii) imperfections present in the reaction spot; and (b) at least one algorithm for calculating the concentration of the analyte contained in the sample.

Analyte detection devices and methods with hematocrit/volume correction and feedback control

Disclosed are devices, arrangements and methods for quantifying the concentration of an analyte present in bodily fluid, including: an assay pad having at least one chemical reagent capable of producing a detectable signal in the form of a reaction spot upon reaction with the analyte; a light source; a detector array; a processor; and a memory in communication with the processor, the memory comprising: (a) at least one value indicative of one or more of: (i) the level of hematocrit contained in the sample; (ii) the volume of the sample applied to the assay pad; or (iii) imperfections present in the reaction spot; and (b) at least one algorithm for calculating the concentration of the analyte contained in the sample.

Automated detection of positive assay regions in microfluidic devices

提供了一种在包括一个或多个回路元件的微流体装置中自动检测测定阳性的测定区域的方法。该方法包括：收集自动识别的测定区域(570、572)的一组数字图像，其中至少部分地基于所述一个或多个回路元件(522)的尺寸来识别所述自动识别的测定区域；基于所述自动识别的测定区域的所述组数字图像，计算在全部或部分测定过程中的变化率；将所述变化率与阈值比较；以及如果所述变化率大于所述阈值，将该自动识别的测试区域确定为测试阳性。

Reader devices for optical and electrochemical test devices

This invention relates generally to devices and methods for performing optical and electrochemical assays and, more particularly, to devices having optical and electrochemical detectors and to methods of performing optical and electrochemical assays using such devices. The present invention is particularly useful for performing immunoassays and/or electrochemical assays at the point-of-care.

Assay test device, kit and method of using

本发明涉及分析试验装置，和用于监测、感测、阅读和显示结果的方法和试剂盒，通过使用具有诸如电池、阅读装置和其它电路的印刷电子元件的装置和/或通过使用将敏感指示剂pH染料用于测试的比色工具，或通过两者。

Analyte detection devices and methods with hematocrit/volume correction and feedback control

Molecular diagnostic assay device and method of use

point-of-care (poc) analytical testing system and method for increasing reader performance

"sistema de teste analítico de ponto de atendimento (poc) e método para aumentar o rendimento de um leitor do mesmo", por tratar a invenção de um sistema de teste de ponto de atendimento (poc) compreendendo um leitor (500) tendo uma incubadora (530) localizada no interior do alojamento (504) do leitor (500), a incubadora (530) tendo um rotor (534) suportado para rotação e tendo uma pluralidade de fendas (538) arranjadas circunferencialmente. um mecanismo de acionamento (550) é configurado para rotacionar o rotor (534) em torno de um eixo central. uma pluralidade de elementos de teste analíticos é dimensionada para ser encaixada nas fendas (538) da incubadora (530), tanto manualmente como por demanda. cada elemento de teste analítico compreende normalmente um suporte (102, 206, 264, 304) no interior de um cartucho (140, 204). o suporte (102, 206, 264, 304) retém pelo menos um de um chip de química seca (360) compreendendo uma camada porosa de espalhamento (184) disposta em relação empilhada com pelo menos uma camada reagente (188) ou um dispositivo de ensaio de fluxo lateral (1, 20, 100, 320), sendo que a pluralidade de elementos de teste pode assumir um fator de forma comum com capacidade multiplexada, e cujos cartuchos (140, 204) são preferencialmente fechados para permitir um processamento por meio de acesso randômico. "point-of-care (poc) analytical testing system and method for increasing the performance of a reader of the same", for treating the invention of a point-of-care (poc) testing system comprising a reader (500) having an incubator (530) located within the housing (504) of the reader (500), the incubator (530) having a rotor (534) supported for rotation and having a plurality of circumferentially arranged slots (538). a drive mechanism (550) is configured to rotate the rotor (534) about a central axis. a plurality of analytical test elements are sized to fit into the slots 538 of the incubator 530, either manually or on demand. each ...

Colorimetric analyzer with de-bubbling

A colorimetric analyzer includes a reaction chamber configured to receive a sample and at least one reagent. A measurement cell is operably coupled to the reaction chamber. The measurement cell has an illumination source and an illumination detector spaced from the illumination source such that illumination from the illumination source passes through the reacted sample to the illumination detector. A controller is coupled to the illumination source and the illumination detector. The controller is configured to generate an analytic output based on a signal from the illumination detector. A fill conduit is operably interposed between the reaction chamber and the measurement cell. The fill conduit is configured to reduce bubbles.

Method for quantifying endotoxins from a biological sample

Un procédé de quantification d'endotoxines d'un échantillon biologique avec un support d'analyse introduit dans le champ de vue d'un imageur, ledit support d'analyse comprenant au moins une chambre d'analyse et une pluralité de chambres de référence, comprenant, pour chacun d'une pluralité d'instants de mesure d'une durée de mesure : a) acquisition (S02) d'une image et détermination (S03) de valeurs d'intensité lumineuse des chambres de référence et d'analyse ; b) détermination (S04) d'une relation d'étalonnage liant valeur d'intensité lumineuse et concentration à cet instant de mesure ; c) détermination (S05) d'au moins une concentration d'endotoxines dans l'échantillon biologique à partir de ladite relation d'étalonnage et de la valeur d'intensité de la chambre d'analyse audit instant de mesure ; le procédé comprenant ensuite d) une détermination (S06) d'une évolution temporelle d'une concentration d'endotoxines dans l'échantillon biologique au cours de la durée de mesure à partir de concentration d'endotoxines pour plusieurs instants de mesure. Figure pour l'abrégé : figure 3 A method for quantifying endotoxins from a biological sample with an analysis support introduced into the field of view of an imager, said analysis support comprising at least one analysis chamber and a plurality of reference chambers, comprising, for each of a plurality of instants of measurement of a measurement duration: a) acquisition (S02) of an image and determination (S03) of light intensity values of the reference and analysis chambers; b) determination (S04) of a calibration relation linking light intensity value and concentration at this instant of measurement; c) determination (S05) of at least one concentration of endotoxins in the biological sample from said calibration relationship and the intensity value of the analysis chamber at said instant of measurement; the method ...

Apparatus and method for multiple reflection solution immersed silicon biosensor

Consumer-based disease diagnostics

A diagnostic system performs a disease diagnostic test using at least an optical property modifying device and a mobile device. A user provides a biological sample to the optical property modifying device. The biological sample reacts with a reagent in reaction chambers of the device. The user captures images of the optical property modifying device using the mobile device. Based on an analysis of the captured images, the diagnostic system can determine the result of the disease diagnostic test. The diagnostic system presents the test result to the user via the mobile device.

Analyte detection devices and methods with hematocrit/volume correction and feedback control

An arrangement (10) for measuring the concentration of an analyte contained in a sample of body fluid includes: an assay pad (40) comprising at least one chemical reagent capable of producing a detectable signal in the form of a reaction spot (50) formed upon reaction with the analyte; a light source (32); a detector array (20); a processor (84); and a memory (80) in communication with the processor. The memory (80) includes at least one value indicative of one or more of: (i) the level of hematocrit contained in the sample; (ii) the volume of the sample applied to the assay pad; or (iii) imperfections present in the reaction spot; and further including at least one algorithm for calculating the concentration of the analyte contained in the sample. Alternatively, an arrangement (10) is described for measuring the concentration of an analyte contained in a sample of body fluid, the arrangement including: an assay pad (40) comprising at least one chemical reagent capable of producing a detectable signal in the form of a reaction spot (50) formed upon reaction with the analyte; a light source(32); a detector array (20); a processor (84); a memory (80) in communication with the processor; and at least one catalyst device constructed and arranged to be responsive to control signals.

Method for detection of legionella bacteria employing purified antigen-specific antibodies

Essentially protein-free O-carbohydrate or O-polysaccharide antigens are separated from a species, or serogroup of a species, of bacteria of the genus Legionella, and coupled to an activated chromatographic column. The column so prepared is used to purify raw antibodies to the same species or serogroup of a species, of Legionella. The resulting antigen-specific antibodies are used in immunochemical assays for detecting Legionnaires disease, Pontiac fever and other Legionella-caused diseases in humans and for detecting Legionella bacteria in environmental samples. A rapid, highly specific and sensitive immunoassay for Legionella pneumophila serogroup 1 conducted in an immunochromatographic test device is described in detail.

Consumer-based disease diagnostics

Optical sensor

光学的センサは、投入部と、固定部と、判定部とを有する。投入部は上側に設けられている。試料中に含まれるアナライトと反応するアクセプタを固定した担体が配置される固定部は、投入部の下方に設けられている。判定部は、第１金属層と、第２金属層と、中空領域とを含む。第１金属層は電磁波が供給されるように形成されている。第２金属層は第１金属層に対向している。中空領域は第１金属層と第２金属層とに挟まれている。投入部と固定部と中空領域は、投入部から中空領域へ向かって試料が流れる流路の一部を構成している。 The optical sensor includes an input unit, a fixed unit, and a determination unit. The input part is provided on the upper side. The fixing part on which the carrier that fixes the acceptor that reacts with the analyte contained in the sample is arranged is provided below the input part. The determination unit includes a first metal layer, a second metal layer, and a hollow region. The first metal layer is formed so as to be supplied with electromagnetic waves. The second metal layer faces the first metal layer. The hollow region is sandwiched between the first metal layer and the second metal layer. The input portion, the fixed portion, and the hollow region constitute a part of the flow path through which the sample flows from the input portion toward the hollow region.

Systems and methods for reagentless test strips

A system for detecting an analyte with a reagentless dry test strip includes a collector for collecting a blood sample from a user. The system additionally includes a mixer for receiving the collector and mixing the blood sample. The system additionally includes reagents, located in the mixer, for mixing with the blood sample. The system additionally includes a dry test strip for receiving the blood sample mixed with the reagents.

Without reagent test strip system and method

用无试剂干测试条检测分析物的系统包括收集器，用于从用户收集血液样品。所述系统还包括混合器，用于接收收集器并混合血液样品。所述系统还包括试剂，其位于混合器中，用于与血液样品混合。所述系统还包括干测试条，用于接收与试剂混合的血液样品。

Multi-layered devices for analyte detection

A multi-layered device for detecting the presence or absence of an analyte within a test sample is described. The device includes a sensing layer and a control layer. The sensing layer is configured to support a reaction so as to exhibit a signal indicative of the presence or absence of the analyte in the test sample. The control layer is in fluid communication with and vertically adjacent to the sensing layer and includes a reagent capable of inhibiting the reaction and/or other unwanted side reactions at the sensing layer after a certain period of time by diffusive movement of the reagent from the control layer to the sensing layer.

System for detection of nucleic acids

System, including methods, apparatus, and kits, for detection of nucleic acids.

A method for detecting optical phase changes during biosensor operation, biosensing apparatus and a biosensor adapted for use in the same

La présente invention se rapporte à un appareil de biocaptage comprenant: un biocapteur optique résonant, dont une couche de revêtement est partiellement sensibilisée à au moins une espèce d'examen donnée et est configurée de façon à produire une diffraction de la lumière; une source lumineuse étendue; un organe de collimation destiné à diriger la lumière depuis la source lumineuse étendue sur le biocapteur optique couplé resonant; un détecteur de lumière; un organe de focalisation de la lumière destiné à diriger la lumière diffractée depuis la couche sensibilisée configurée sur le détecteur de lumière, afin de définir un champ de diffraction dans le plan de détecteur; et un organe de balayage destiné à balayer le détecteur de lumière par rapport à ce champ de diffraction. La présente invention se rapporte également à un procédé permettant de détecter les changements de phase optique pendant le fonctionnement du biocapteur. The present invention relates to a biosensing apparatus comprising: a resonant optical biosensor, a coating layer of which is partially sensitized to at least one given examination species and is configured to produce light diffraction; an extended light source; a collimator for directing light from the extended light source onto the resonant coupled optical biosensor; a light detector; a light focusing member for directing light diffracted from the sensitized layer configured on the light detector, to define a diffraction field in the detector plane; and a scanning member for scanning the light detector with respect to this diffraction field. The present invention also relates to a method for detecting optical phase changes during operation of the biosensor.

Assay test device, kit and method of using

본 발명은 배터리, 판독 장치, 및 기타 회로소자와 같은 인쇄 전자를 갖는 장치를 사용함에 의한 및/또는 민감성 인디케이터 pH 염료를 이용하여 시험하기 위한 비색 수단을 사용함에 의한, 또는 양자에 의한 결과의 모니터, 감지, 판독 및 디스플레이용 분석 시험 장치, 및 방법 및 키트에 관한 것이다. The present invention provides monitoring of results by using devices with printed electronics, such as batteries, reading devices, and other circuitry, and/or by using colorimetric means for testing with sensitive indicator pH dyes, or both. , assay devices for sensing, reading and display, and methods and kits.

Specimen reader system based on accurate colorimetry

Optical sensor

An optical sensor assembly includes a light source, a surface plasmon-sensitive structure for reflecting light, a light detector, and a signal indicator. The light detector receives light that is reflected from the surface plasmon-sensitive structure at an angle which is sensitive to surface plasmon absorption.

Device for determining at least one analyte capable of being contained in a liquid sample

本发明涉及用于测定至少一种能够包含在液体样品中的分析物的存在和/或量的装置，所述装置包含毛细扩散工具(2)，在其上实现用于存放液体样品的区(3)、上游释放区(4)和至少两个捕获区(5)。装置(1)也包含位于所述捕获区(5)中的至少一个的下游的至少一个下游释放区(6)，所述下游释放区(6)包含与可见的和/或可测量的标记物缀合的至少一种检测试剂；并且释放区(4、6)的检测试剂和/或位于所述释放区(4、6)的直接下游的额外的捕获区(5)的捕获试剂，适于特异性结合所述分析物和/或彼此特异性结合，以形成复合物，所述复合物使得能够在所述(一个或多个)额外的捕获区处测定所述液体样品中的所述分析物。

Human body biochemical detection sensing chip

本发明公开了一种人体生化检测传感芯片，包括人体血液或组织液采集机构和人体血液或组织液体外处理机构，所述人体血液或组织液采集机构包括单根中空微针或由多根中空微针排列组成的微针阵列，所述人体血液或组织液体外处理机构之中设有若干条微通道和若干个检测腔，各条所述微通道分别以人体血液或组织液采集机构为中心而螺旋盘绕设置且一端与单根或多根中空微针的针头开口相连通而另一端与其中一个检测腔相连通，各条所述微通道和各个检测腔内分别涂覆有可与待检测的物质结合、反应或者促进反应的物质，能够将微流控芯片与微针结合于一体，解决现有人体生化传感技术中人体生物质的采集及其处理过程中的环境和时效问题。

Definitive development diagnostic analysis

Detecting a definitive presence or absence of an analyte from a borderline test is shown and described. In one embodiment, a method of generating a definitive test result includes continuously incubating the assay concuiTently with continuously reading a diagnostic test on the assay. In particular examples, reading the diagnostic test on the assay includes performing a one minute diagnostic reading. Typically, when a borderline test result is detected, further development of the assay is performed. In most examples, detecting a definitive presence or absence test result deactivates the testing sequence.

Analyte detection device and method with hematocrit/volume correction