Cell analyzer, cell processing apparatus, specimen preparing apparatus

The present invention provides a cell analyzer which comprises: a cell dispersion unit which causes aggregated cells in a biological specimen to be dispersed, through a shearing force applying process of applying a shearing force to the aggregated cells and an ultrasonic dispersion process of dispersing the aggregated cells, by using ultrasonic waves; a detection unit which detects characteristics information reflecting properties of the cells in the biological specimen on which the shearing force applying process and the ultrasonic dispersion process have been performed; and an analysis unit which analyzes the cells in the biological specimen, based on a detection result from the detection unit.

MAGNETIC SEPARATION DEVICE AND METHOD OF USE

The current invention relates to the method and apparatus to magnetically separate biological entities with magnetic labels from a fluid sample. The claimed magnetic separation device removes biological entities with magnetic labels from its fluidic solution by using a soft-magnetic center pole with two soft-magnetic side poles. The claimed device further includes processes to dissociate entities conglomerate after magnetic separation. 1. A device for separating biological entities comprising:a magnetic field source having a first surface and a second surface;a soft magnetic center pole having a first end contacting said first surface, and a tip end tapering away from said first end, thereby concentrating magnetic flux from said first surface towards said tip end;a first soft magnetic side pole having a first base end and a second end;a second soft magnetic side pole having a second base end and a third end;a channel contacting said tip end contains a fluidic sample comprising said biological entities, thereby causing separation of said biological entities bound with magnetic labels towards said tip end;wherein said first and second base ends contact said second surface;wherein said second and third ends are located on opposing sides of said tip end;wherein said magnetic flux from said tip end divides into each of said second and third ends, thereby forming a flux closure within said center pole and said first and second side poles; andwherein said biological entities bound with magnetic labels are removed from said channel after separating said channel from said tip end.2. The device according to claim 1 , wherein said magnetic field source comprises a permanent magnet having a first polarization surface contacting a top surface of a soft magnetic shield claim 1 , and a second polarization surface opposing said first polarization surface claim 1 , wherein said first surface is said second polarization surface and said second surface is said top surface.3. The ...

DEVICES, SYSTEMS AND METHODS FOR SAMPLE PREPARATION

Devices, systems and methods including a sonicator for sample preparation are provided. A sonicator may be used to mix, resuspend, aerosolize, disperse, disintegrate, or de-gas a solution. A sonicator may be used to disrupt a cell, such as a pathogen cell in a sample. Sample preparation may include exposing pathogen-identifying material by sonication to detect, identify, or measure pathogens. A sonicator may transfer ultrasonic energy to the sample solution by contacting its tip to an exterior wall of a vessel containing the sample. Multipurpose devices including a sonicator also include further components for additional actions and assays. Devices, and systems comprising such devices, may communicate with a laboratory or other devices in a system for sample assay and analysis. Methods utilizing such devices and systems are provided. The improved sample preparation devices, systems and methods are useful for analyzing samples, e.g. for diagnosing patients suffering from infection by pathogens. 1. A system configured to assay a sample for the presence of pathogen-identifying material , comprising:i) a device configured to assay a sample for the presence of pathogen-identifying material, said device comprising a housing, and within said housing, a sonicator having a sonicator tip; a vessel holder configured to hold a vessel having a vessel wall; a sample handling system configured to move said vessel within the housing, wherein the vessel is configured to hold said sample for sonication; a solenoid disposed to urge said sonicator tip towards the vessel wall when said solenoid is activated, wherein the sonicator tip is configured to contact the vessel wall effective to transfer ultrasonic energy from said sonicator to the vessel wall upon operation of the sonicator; a spring disposed to as to urge the sonicator tip away from the vessel wall; and a detector andii) a communication assembly configured to communicate with one or more of a user, another device, a laboratory ...

Hifu induced cavitation with reduced power threshold

An apparatus for irradiating a liquid sample with acoustic energy to generate cavitation in the liquid sample. The apparatus includes a source for emitting high intensity ultrasound waves and is adapted to receive a cartridge having a chamber containing the liquid sample, wherein the high intensity ultrasound waves are focused on a focus position at or near a nucleation element extending at least partially into the chamber.

Microfluidic Chip for Acoustic Separation of Biological Objects

A microfluidic device for sorting biological objects includes a microfluidic chip including a planar substrate having first and second planar surfaces, the planar substrate including first and second networks of channels recessed respectively from the first and second planar surfaces and fluidically connected by way of at least a through-hole in the planar substrate; a first lid attached to the first planar surface of the planar substrate and substantially covering the first network of channels; and a second lid attached to the second planar surface of the planar substrate and substantially covering the second network of channels; and one or more piezoelectric transducers attached to the first lid and/or the second lid and configured to generate first and second acoustic standing waves in a first linear channel of the first network of channels and a second linear channel of the second network of channels, respectively. 1. A microfluidic device for sorting biological objects comprising:{'claim-text': ['a planar substrate having first and second planar surfaces, the planar substrate including first and second networks of channels recessed respectively from the first and second planar surfaces and fluidically connected in series or parallel by way of at least a through-hole in the planar substrate;', 'a first lid attached to the first planar surface of the planar substrate and substantially covering the first network of channels; and', 'a second lid attached to the second planar surface of the planar substrate and substantially covering the second network of channels; and'], '#text': 'a microfluidic chip including:'}one or more first piezoelectric transducers attached to an exterior of the first lid and configured to generate a first acoustic standing wave having a first wavelength in a first linear channel of the first network of channels.2. The microfluidic device of further comprising one or more second piezoelectric transducers attached to an exterior of the second ...

Devices, systems and methods for sample preparation

Devices, systems and methods including a sonicator for sample preparation are provided. A sonicator may be used to mix, resuspend, aerosolize, disperse, disintegrate, or de-gas a solution. A sonicator may be used to disrupt a cell, such as a pathogen cell in a sample. Sample preparation may include exposing pathogen-identifying material by sonication to detect, identify, or measure pathogens. A sonicator may transfer ultrasonic energy to the sample solution by contacting its tip to an exterior wall of a vessel containing the sample. Multipurpose devices including a sonicator also include further components for additional actions and assays. Devices, and systems comprising such devices, may communicate with a laboratory or other devices in a system for sample assay and analysis. Methods utilizing such devices and systems are provided. The improved sample preparation devices, systems and methods are useful for analyzing samples, e.g. for diagnosing patients suffering from infection by pathogens.

Automatic Apparatus of Screening Disease/Cancer in Vitro

An automatic apparatus is provided for screening disease/cancer in vitro. The apparatus comprises a filling device; a gripping device at a side of the filling device; a carrying platform correspondingly set under the filling device and the gripping device; a containing device on the carrying platform; at least two receiving slots on the carrying platform; a placing device on the carrying platform; a shaking device on the carrying platform; and a detecting device on the carrying platform. Thus, the apparatus is provided for molecular biological researches, biochemical examinations, radioimmunoassay and drug developments with reduced human labor, saved operating time, avoided experimental errors and enhanced experimental accuracy and efficiency. 1. An automatic apparatus of screening disease/cancer in vitro , comprisinga filling device;a gripping device, said gripping device being located at a side of said filling device;a carrying platform, said carrying platform being correspondingly located under said filling device and said gripping device;a containing device, said containing device being located on said carrying platform;at least two receiving slots, said receiving slots being located on said carrying platform;a placing device, said placing device being located on said carrying platform;a shaking device, said shaking device being located on said carrying platform; anda detecting device, said detecting device being located on said carrying platform.2. The automatic apparatus according to claim 1 ,wherein said filling device comprises a first filling unit and a second filling unit.3. The automatic apparatus according to claim 2 ,wherein said first filling unit comprises four dispensers.4. The automatic apparatus according to claim 2 ,wherein said second filling unit comprises ninety six dispensers.5. The automatic apparatus according to claim 1 ,wherein said gripping device is an automatic robotic arm.6. The automatic apparatus according to claim 1 ,wherein said ...

SYSTEMS AND METHODS FOR PROCESSING DRILL CUTTINGS

An example system may be used to automate one or more processes relating to drill cutting handling, cleaning, and packing to produce consistently high-quality cleaned samples. In some implementations, an example system may employ cleaning processes including centrifugal and ultrasonic processes to clean a sample automatically without the aid of a human operator thereby increasing efficiency and quality of the produced samples for better subsequent sample analysis. A system may include an unloading module, a cleaning module, and packing module, all of which may be operated in combination with a robotic arm. 1. A system for processing a sample comprising drill cuttings from a wellbore in a rock formation , the system comprising:an unloading module for transferring the sample from a collection container to a sample tube, the unloading module comprising a funnel and an unloading module camera for reading a label on the collection container;a cleaning module for cleaning a sample, the cleaning module comprising a tank comprising a centrifuge for centrifuging at least one sample tube;a packing module for packaging a sample, the packing module comprising a tray for receiving the sample form the at least one sample tube, a packing module camera for imaging the sample, and a packing and labeling device for packaging the sample; anda robotic arm for transferring the sample tube between the unloading module, the cleaning module, and the packing module, the robotic arm comprising a robotic arm camera.2. The system of claim 1 , where the unloading module comprises a water jet nozzle for discharging a water jet to drive the sample from the collection container to the funnel.3. The system of claim 1 , where the sample tube comprises an opening and a removable perforated top cap at a proximal end and an opening and a removable perforated bottom cap at a distal end claim 1 , and where the unloading module is arranged such that the sample tube receives the sample from the funnel ...

Automatic Analyzer and Analysis Method

Provided is an automatic analysis technique that prevents liquid contained in a reaction container from locally contacting with liquid added afterward and has less occurrence frequency of equipment malfunction and high performance. An automatic analyzer () includes: a reaction container disk () that holds a reaction container (); a stirring mechanism () that stirs liquid contained in the reaction container () in a non-contact manner; a carrying mechanism () that carries the reaction container () between the reaction container disk () and the stirring mechanism (); a reagent disk () that holds a reagent container (); and a reagent dispensing mechanism () that suctions and discharges a reagent contained in the reagent container (), the stirring mechanism () is provided at a position where the reagent dispensing mechanism () discharges the reagent, and the reagent dispensing mechanism () discharges the reagent, which is sucked from the reagent container (), to the reaction container () installed in the stirring mechanism (). 1. An automatic analyzer comprising:a reaction container disk that holds a reaction container;a stirring mechanism that stirs liquid contained in the reaction container in a non-contact manner;a carrying mechanism that carries the reaction container between the reaction container disk and the stirring mechanism;a reagent disk that holds a reagent container; anda reagent dispensing mechanism that suctions and discharges a reagent contained in the reagent container, whereinthe stirring mechanism is provided at a position where the reagent dispensing mechanism discharges the reagent, andthe reagent dispensing mechanism discharges the reagent, which is sucked from the reagent container, to the reaction container installed in the stirring mechanism.2. The automatic analyzer according to claim 1 , whereinthe reagent dispensing mechanism starts to discharge the reagent after the stirring mechanism starts to stir the liquid contained in the reaction ...

CARTRIDGES, ANALYZERS, AND SYSTEMS FOR ANALYZING SAMPLES

A cartridge, analyzer for use therewith, and system including the cartridge and analyzer. The cartridge is configured to receive a biological fluid to be analyzed and includes a plate defining a main channel, a hemolysis chamber, and an oximetry chamber consecutively interconnected with one another. The analyzer is configured to perform analysis of sample disposed within the main channel, hemolyze sample disposed within the hemolysis chamber, and perform oximetry on sample disposed within the oximetry chamber. The cartridge and analyzer may further include alignment and clamping structures for maintain the cartridge in fixed position and alignment during testing or may further include cooperating features for moving the cartridge relative to the analyzer into a testing position or between various different testing positions. 1. A cartridge configured to receive a blood sample to be analyzed , comprising:a plate defining, on a face surface thereof, a main channel, a hemolysis chamber disposed in fluid communication with the main channel and configured to facilitate hemolysis of sample disposed within the hemolysis chamber, an oximetry chamber configured to facilitate oximetry of sample disposed within the oximetry chamber, and an interconnection channel coupling the hemolysis chamber and oximetry chamber to one another in fluid communication so as to define a sample flow path from the main channel through the hemolysis chamber and the oximetry chamber.2. The cartridge according to claim 1 , further including a plurality of sensors operably positioned adjacent to and aligned with the main channel defined within the plate.3. The cartridge according to claim 2 , wherein the sensors are chemical fluorescence sensors or ion selective electrodes.4. The cartridge according to claim 1 , further including a flexible membrane claim 1 , at least a portion of the flexible membrane disposed adjacent the hemolysis chamber and configured to transmit ultrasonic energy to sample ...

Devices, systems and methods for sample preparation

Devices, systems and methods including a sonicator for sample preparation are provided. A sonicator may be used to mix, resuspend, aerosolize, disperse, disintegrate, or de-gas a solution. A sonicator may be used to disrupt a cell, such as a pathogen cell in a sample. Sample preparation may include exposing pathogen-identifying material by sonication to detect, identify, or measure pathogens. A sonicator may transfer ultrasonic energy to the sample solution by contacting its tip to an exterior wall of a vessel containing the sample. Multipurpose devices including a sonicator also include further components for additional actions and assays. Devices, and systems comprising such devices, may communicate with a laboratory or other devices in a system for sample assay and analysis. Methods utilizing such devices and systems are provided. The improved sample preparation devices, systems and methods are useful for analyzing samples, e.g. for diagnosing patients suffering from infection by pathogens.

CARTRIDGES, KITS, AND METHODS FOR AMPLIFICATION AND DETECTION OF ANALYTES

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample. 1. A sample analysis cartridge comprising:an input tunnel that extends from an aperture, the input tunnel configured to permit insertion of a sample collection device having a distal portion adapted to be exposed to a sample comprising a target nucleic acid;a reservoir configured to hold a fluid, the reservoir further configured to receive the sample from the sample collection device such that the sample is mixed with an effective amount of reagents for amplification of the target nucleic acid in the fluid of the reservoir to generate amplicons comprising signaling agents;an analysis channel configured to receive the fluid comprising the amplicons from the reservoir; anda sensor at least partially disposed in the analysis channel such that the amplicons indicative of at least one of a presence, absence, or quantity of the target nucleic acid in the sample localize over the sensor and the sensor senses signals indicative of at least one of the presence, absence, or quantity of the target nucleic acid in the sample based on reactions between a substrate and the signaling agents of the amplicons localized over the sensor.2. The sample analysis cartridge of claim 1 , further comprising reagent ball comprising the reagents for ...

PHASE-MODULATED STANDING WAVE MIXING APPARATUS AND METHODS

Disclosed are mixing apparatus adapted to provide mixing of components in an automated analyzer. The mixing apparatus includes a reservoir configured to contain a coupling liquid, a transducer configured to be driven at a frequency and communicate with the coupling liquid, and a signal generation unit configured to provide a phase modulatable drive signal to the transducer. In some embodiments, improved patient sample and reagent mixing may be provided. Systems and methods are provided, as are other aspects. 1. A sonic mixing apparatus , comprising:a reservoir configured to contain a coupling liquid;a transducer configured to be driven at a frequency and communicate with the coupling liquid; anda signal generation unit configured to provide a phase modulatable drive signal to the transducer.2. The sonic mixing apparatus of claim 1 , further comprising a reaction vessel suspended in the reservoir from a carrier member.3. The sonic mixing apparatus of claim 1 , wherein the reservoir comprises an annular recess.4. The sonic mixing apparatus of claim 1 , wherein the signal generation unit is configured to be driven at approximately a resonant frequency of the transducer.5. The sonic mixing apparatus of claim 1 , wherein the signal generation unit comprises one or more oscillator.6. The sonic mixing apparatus of claim 1 , wherein the signal generation unit is configured to modulate a phase of the phase modulatable drive signal between a first phase angle and a second phase angle which is different than the first phase angle.7. The sonic mixing apparatus of claim 1 , wherein the signal generation unit includes an oscillator operable at between about 200 KHz and about 4 MHz.8. The sonic mixing apparatus of claim 1 , wherein the signal generation unit is configured to adjust a phase of the phase modulatable drive signal between about 0 degrees and about 180 degrees.9. The sonic mixing apparatus of claim 1 , wherein the signal generation unit comprises a phase modulator ...

CARTRIDGES, KITS, AND METHODS FOR AMPLIFICATION AND DETECTION OF ANALYTES

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Method to detect pre-symptom early cancer with enhanced accuracy

The current invention relates to the method to detect early stage pre-symptom cancer with enhanced accuracy. The claimed methods use anti-tumor agent to cause lysis of any existing cancer cells in a person, and causing release of genetic material from the lysed cancer cells into the blood stream of the person. The claimed methods further utilize a PCR method to amplify cancer gene in released generic material from a blood plasma sample of the person. The claimed methods further perform genetic sequencing on the blood plasma sample after PCR to identify existence of the cancer genes, thereby confirming existence of cancer.

N-NITROSAMINE DETERMINATION IN AQUEOUS SAMPLES WITH SONICATION AND MICROEXTRACTION

An automated dispersive liquid-liquid microextraction method of detecting and quantifying N-nitrosamines in an aqueous sample. The method includes (a) extracting an aqueous solution comprising the N-nitrosamines by mixing an extraction solvent and a dispersive solvent with the aqueous solution, such that the N-nitrosamines, or a portion thereof, re-distribute from the aqueous solution to the extraction solvent, (b) permitting the resulting mixture in (a) to form a two-phase mixture comprising an aqueous phase comprising the aqueous solution with reduced amounts of the N-nitrosamines and an organic phase comprising the extraction solvent with the N-nitrosamines extracted from the aqueous solution, (c) injecting the organic phase, or a portion thereof, into an injection port of a gas chromatograph coupled with at least one mass spectrometer, and (d) analyzing the N-nitrosamines by gas chromatography and mass spectrometry to detect and quantify the concentration of the N-nitrosamines in the aqueous solution. 1. An automated dispersive liquid-liquid microextraction method of detecting and quantifying one or more N-nitrosamines , comprising:(a) extracting an aqueous solution comprising the one or more N-nitrosamines by mixing an extraction solvent and a dispersive solvent with the aqueous solution, wherein the aqueous solution is contained in a sample container, wherein the one or more N-nitrosamines, or a portion thereof, re-distribute from the aqueous solution to the extraction solvent, wherein the mixing comprises sonicating the sample container containing the extraction solvent, the dispersive solvent, and the aqueous solution in a water bath,(b) permitting the resulting mixture in (a) to form a two-phase mixture comprising an aqueous phase comprising the aqueous solution with reduced amounts of the one or more N-nitrosamines and an organic phase comprising the extraction solvent with the one or more N-nitrosamines extracted from the aqueous solution,(c) injecting ...

Cartridges, kits, and methods for amplification and detection of analytes

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Automatic analysis device and automatic analysis method

The automatic analysis device 100 is provided with: a specimen dispensing mechanism 101 that dispenses subject blood plasma and/or normal blood plasma to be added to correct the coagulation time of the subject blood plasma, into a plurality of specimen containers 103; reaction containers 104 that contain the subject blood plasma and/or the normal blood plasma; a reagent dispensing mechanism 106 that dispenses a reagent into the reaction containers 104; and a detecting unit 113 which applies light from a light source 115 to the subject blood plasma and/or the normal blood plasma to which the reagent is added in the reaction containers 104, and which measures the coagulation time on the basis of the obtained scattered light and/or transmitted light.

Ultrasonic horn

An ultrasonic horn in accordance with the present invention comprises a horn body having a longitudinal transmission axis, a proximal end attachable at least indirectly to a source of ultrasonic vibrations, and a distal end with an active end face. The end face is provided with a plurality of fingers projecting parallel to one another and to the axis.

Ultrasonic horn

An ultrasonic horn in accordance with the present invention comprises a horn body having a longitudinal transmission axis, a proximal end attachable at least indirectly to a source of ultrasonic vibrations, and a distal end with an active end face. The end face is provided with a plurality of fingers projecting parallel to one another and to the axis.

用于细胞裂解和提纯的旋转平台以及使用方法

具有感兴趣的细胞组分的磁珠在托盘中的处理凹部序列之间平移、而无需移液。所述圆形托盘包含各自通过相应通道互连的一个或多个凹部序列。所述托盘围绕中心轴线旋转，并且提供在所述托盘外部的磁体、搅拌器和加热器以分别实现磁珠平移、混合和温育。接近凹部的磁体形成珠簇。对托盘的旋转和升高的操纵导致所述簇从一个凹部平移通过通道并且进入到相邻凹部中。包含簇的凹部可以旋转地定位在搅拌器前面，搅拌器延伸成与所述凹部接触、随后进行机械搅拌。安置在托盘下方的加热器可以接受降低到其上的凹部以进行选择性加热。

Auto sampling system

The present invention relates to an anto-sampling system, which comprises: an auto-sampler having a base part, a guide support part disposed on the base part, a first robot arm movably disposed in the X axis direction along the guide support part, and a second robot arm movably disposed in the Y axis direction along the first robot arm; and a module part disposed to be movable in the Z axis direction along the second robot arm, wherein the module unit includes a sampling needle for sucking a sample and an ultrasonic dispersion module for homogenizing the sample.

Stirring determination device, stirring determination method, and analyzer

A stirring determination device, a stirring determination method, and an analyzer capable of determining the quality of stirring of liquid performed by acoustic wave generation means. The stirring determination device (30), the stirring determination method, and the analyzer determine the quality of stirring performed by a stirring device (28) for stirring liquid in a container (5) by acoustic waves generated by an acoustic wave generation element (23) installed on the container (5). The stirring determination device (30) has a temperature sensor (36) for measuring the temperature of the liquid and also has a determination section (16) for determining the quality of stirring of the liquid in the container based on the temperatures of the liquid measured by the temperature sensor at least before and after the stirring.

Sample preparation and delivery system employing external sonicator

A particulate sample mixing and delivery apparatus externally sonically mixes respective samples of particulate stored in storage containers that are sequentially indexed by a carousel prior to being dispensed into an analyzer reservoir. Each container is compliant to acoustic energy emitted by a sonicator unit that is placed against an external surface of a respective container to which preferably a carrier fluid and surfactant are controllably supplied. After sonication, the container is indexed to a sample dispensing location adjacent to the analyzer reservoir. A tiltable cradle then rotates the container to rapidly dispense its mixed contents into the reservoir, and then discards the empty container.

Sonication of a medium

通过在传感器与容器之间耦合的声导杆将声波从该传感器应用至该容器来提供在例如免疫测定中对培养基的声处理，该培养基被容纳于该容器中。该声导杆具有支撑该容器的凹进部分，该凹进部分由被狭缝分开的并配置于该凹进部分周围的声导杆的多个突出部分的刮削表面形成。通过与该容器干接触将该声导杆与该容器耦合，且在两者之间没有任何耦合层。这样的声导杆的应用提供了实现有效的具有相对低能量损失和低温度升高的声处理的优点。

Microfabricated reactor

An integrated microfabricated instrument for manipulation, reaction and detection of microliter to picoliter samples. The instrument is suited for biochemical reactions, particularly DNA-based reactions such as the polymerase chain reaction, that require thermal cycling since the inherently small size of the instrument facilitates rapid cycle times. The integrated nature of the instrument provides accurate, contamination-free processing. The instrument may include reagent reservoirs, agitators and mixers, heaters, pumps, and optical or electromechanical sensors. Ultrasonic Lamb-wave devices may be used as sensors, pumps and agitators.

Stirring determination device, stirring determination method, and analyzer

本发明的目的在于提供一种能够对由声波发生单元进行的液体搅拌的好坏进行判断的搅拌判断装置、搅拌判断方法和分析装置。一种对搅拌装置(28)的搅拌的好坏进行判断的搅拌判断装置(30)、搅拌判断方法和分析装置，该搅拌装置(28)利用安装在容器(5)上的声波发生元件(23)所产生的声波对保持在容器中的液体进行搅拌。搅拌判断装置(30)具有温度传感器(36)，其对液体的温度进行测定；以及判断部(16)，其根据温度传感器测出的至少搅拌前后的液体的温度，对保持在容器中的液体的搅拌好坏进行判断。

Cell analysis apparatus and cell analysis method

Automatic pretreatment device

For device, the system and method for sample preparation

本发明提供了用于样品制备的、包括超声仪的装置、系统和方法。超声仪可以用于对溶液进行混合、重悬浮、雾化、分散、分解或脱气。超声仪可以用于使诸如样品中病原体细胞等细胞破裂。样品制备可以包括通过超声处理来暴露病原体标识物质以检测、鉴别或测量病原体。超声仪可以通过使其端头接触包含样品的器皿的外壁来向样品溶液传递超声能量。包括超声仪的多用途装置还包括用于附加行动和测定的进一步组件。装置和包括这样的装置的系统可以与实验室或系统中其他用于样品测定和分析的装置相通信。本发明提供了利用这样的装置和系统的方法。改进的样品制备装置、系统和方法有助于分析样品，例如，用于诊断遭受病原体感染的患者。

Cartridges, kits, and methods for amplification and detection of analytes

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Cartridges, kits, and methods for amplification and detection of analytes

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Cartridges, kits, and methods for amplification and detection of analytes

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Integrated chemical synthesizers

A modular reactor system and method for synthesizing chemical compounds characterized by a uniform temperature throughout the reaction mixture by use of a continuous flow reactor under high pressure. The apparatus includes a number of generic components such as pumps, flow channels, manifolds, flow restrictors, and valves. Modular reactors, separator and analyzers on an assembly board provide a system where a modular reactor unit has an I.D. of up 100 μm to optimize control of residence time within a reaction zone.

Robotic sample preparation system and method

A method of and a robotic system for preparing product samples to be analyzed at a later time has a plurality of processing stations in different locations. Each station performs a different function of a process for preparing a product sample for later chemical analysis. A robot moves a first container between a container storage area and a prepared sample area for storing in a second container a product sample which has been prepared for analysis and in which the robot moves the first container from the container storage area to each of the processing stations in a predetermined sequence and then removes some of the prepared product sample from the first container and dispenses it into the second container which is located in the prepared sample area. A specific application of such a robotic sample preparation system is disclosed for use in preparing tobacco samples for analysis of nicotine and sugar content.

Automatic analysing apparatus

近年来，在自动分析装置的领域中，伴随分析项目的增加，液体的粘性或接触角等性质不同的分析试剂的种类增加，预测今后也进一步增加。而且，用装置内的水稀释浓缩试剂使用等试剂形态也多样化，稀释水的种类也多样化。在这种状况下，提供一种无论相对于哪种项目均能执行充分搅拌的自动分析装置。在被搅拌物的搅拌中，在相同试剂的搅拌操作时，在添加试剂后，在经过一定时间后通过改变搅拌条件从而能够实现。

Automated analyzer

近年来，在自动分析装置的领域中，伴随分析项目的增加，液体的粘性或接触角等性质不同的分析试剂的种类增加，预测今后也进一步增加。而且，用装置内的水稀释浓缩试剂使用等试剂形态也多样化，稀释水的种类也多样化。在这种状况下，提供一种无论相对于哪种项目均能执行充分搅拌的自动分析装置。在被搅拌物的搅拌中，在相同试剂的搅拌操作时，在添加试剂后，在经过一定时间后通过改变搅拌条件从而能够实现。

Stirrer, and analyzer with vessel and stirrer

【課題】エネルギーの伝達効率に優れ、構造が簡単で小型化が可能であり、メンテナンスが容易な攪拌装置、容器および攪拌装置を備えた分析装置を提供すること。 【解決手段】音波を用いて容器５に保持された液体を攪拌する攪拌装置２０、容器５および攪拌装置を備えた分析装置１。攪拌装置２０は、電力を送電する送電体２１と、送電体から送電される電力を受電し、送電体又は電気端子２４ｃの少なくとも一方の配置が変化することで、送電体に対する相対配置が変化し得る電気端子２４ｃと、電気端子２４ｃが受電した電力を変換して液体を攪拌する音波を発生する振動子２４ｂとを備えている。 【選択図】 図４

Automated assay device for disrupting cells in a sample

本发明提供了用于样品制备的、包括超声仪的装置、系统和方法。超声仪可以用于对溶液进行混合、重悬浮、雾化、分散、分解或脱气。超声仪可以用于使诸如样品中病原体细胞等细胞破裂。样品制备可以包括通过超声处理来暴露病原体标识物质以检测、鉴别或测量病原体。超声仪可以通过使其端头接触包含样品的器皿的外壁来向样品溶液传递超声能量。包括超声仪的多用途装置还包括用于附加行动和测定的进一步组件。装置和包括这样的装置的系统可以与实验室或系统中其他用于样品测定和分析的装置相通信。本发明提供了利用这样的装置和系统的方法。改进的样品制备装置、系统和方法有助于分析样品，例如，用于诊断遭受病原体感染的患者。

Method of washing an aspiration probe of an in-vitro diagnostic system, in-vitro diagnostic method, and in-vitro diagnostic system

A method of washing an aspiration probe of an in-vitro diagnostic system is disclosed. The aspiration probe comprises an outer surface and an inner surface forming an inner space for receiving a fluid. The method comprises dipping the aspiration probe into a first wash fluid so that the outer surface is immersed at least in part into the first wash fluid, aspirating an amount of the first wash fluid into the inner space of the aspiration probe, propagating an ultrasonic vibration to the outer surface of the aspiration probe via the first wash fluid, and rinsing the outer surface and the inner surface of the aspiration probe with a second wash fluid. Further, an in-vitro diagnostic method and an in-vitro diagnostic system are disclosed.

Automatic chemical analyzer and electrical impedance spectrum measuring instrument

本发明提供能够在执行搅拌动作之前检测搅拌机构的状态的自动化学分析装置以及电阻抗谱测定器。自动化学分析装置将试剂和作为分析对象的样品分注到反应容器(107)内，通过向反应容器(107)照射声波来进行搅拌。自动化学分析装置具备：压电元件(201)，其产生声波；被设置于压电元件(201)的表面的多个分割电极(211)；功率放大器(203)，其通过对各分割电极(211)施加电压，使压电元件(201)产生声波；通过对各分割电极(211)施加电压来测定与各分割电极(211)有关的电阻抗谱的主计算机和阻抗测定电路。

Automatic analysis device and automatic analysis method

Cavitation induced by high-intensity focused ultrasound with reduced power threshold

FIELD: medicine. SUBSTANCE: group of inventions refers to medicine, namely to molecular diagnostics. A device for exposing a liquid sample to acoustic energy by generating cavitation in a liquid sample comprises a high-intensity ultrasonic wave source and a cartridge comprising a liquid sample and a liquid-air interface. The device is configured to focus high-intensity ultrasonic waves so that the cartridge integrated into the device generates a liquid well head above the liquid-air interface inside the cartridge, while a well head drop is a nucleation element. The group of inventions also refers to a system and a method for exposing the liquid sample to acoustic energy, as well as to a machine-readable medium with a software program for implementing the above method. EFFECT: group of inventions provides probe processing by focused acoustic energy to generate the homogenous cavitation in the sample with reducing the desired energy and power. 9 cl, 9 dwg РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (13) 2 549 443 C2 (51) МПК G01N 33/487 (2006.01) B01F 11/02 (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ОПИСАНИЕ (21)(22) Заявка: ИЗОБРЕТЕНИЯ К ПАТЕНТУ 2011146000/15, 09.04.2010 (24) Дата начала отсчета срока действия патента: 09.04.2010 (72) Автор(ы): ВАН ДОРН Ари Р. (NL), ДЕ ЙОНГ Михил (NL) (73) Патентообладатель(и): Биокартис НВ (BE) Приоритет(ы): (30) Конвенционный приоритет: (43) Дата публикации заявки: 20.05.2013 Бюл. № 14 R U 14.04.2009 EP 09157849.2 (45) Опубликовано: 27.04.2015 Бюл. № 12 2008031094 A1, 07.02.2008. SU 1749825 A1, 23.05.1989. RU 2262706 C2, 20.10.2005 (85) Дата начала рассмотрения заявки PCT на национальной фазе: 14.11.2011 (86) Заявка PCT: 2 5 4 9 4 4 3 (56) Список документов, цитированных в отчете о поиске: JP 2007010345 A, 18.01.2007. US 2 5 4 9 4 4 3 R U (87) Публикация заявки PCT: WO 2010/118539 (21.10.2010) Адрес для переписки: 129090, Москва, ул. Б. Спасская, 25, строение 3, ООО "Юридическая фирма Городисский и Партнеры" (54) ...

DEVICE FOR THE PREPARATION OF BIOLOGICAL SAMPLES

Dispositif comprenant au moins une partie destinée à recevoir au moins un tube apte à contenir un échantillon biologique, ladite partie comportant, un support apte à recevoir et maintenir la partie fixe d'une sonde de sonication, une partie vibrante apte à être accouplée avec la sonotrode d'une sonde de sonication, une partie mobile solidaire du support, apte à se déplacer, de préférence en translation, relativement au support, d'une position de repos vers une position de serrage et inversement, par le biais d'un moyen de serrage, un moyen de chauffage du contenu du tube, de sorte que lorsque la partie mobile est en position de repos, un espace est ménagé entre ladite partie mobile et la partie vibrante permettant de positionner ou retirer ledit au moins un tube, alors que lorsque la partie mobile est en position de serrage en présence d'au moins un tube, ladite partie mobile est en appui contre ledit au moins un tube et exerce une force contre la paroi de ce dernier, répercutée sur la partie vibrante, emprisonnant ainsi ledit au moins un tube, ladite force étant générée par le moyen de serrage sur la partie mobile. Device comprising at least one part intended to receive at least one tube capable of containing a biological sample, said part comprising, a support able to receive and hold the fixed part of a sonication probe, a vibrating part suitable for being coupled with the Sonotrode of a sonication probe, a mobile part integral with the support, able to move, preferably in translation, relative to the support, from a rest position to a clamping position and vice versa, by means of a means clamping means, a means for heating the contents of the tube, so that when the movable part is in the rest position, a space is provided between said movable part and the vibrating part for positioning or removing said at least one tube, whereas when the mobile part is in the clamping position in the presence of at least one tube, said movable part bears ...

CAVITATION INDUCED BY A HIGH-INTENSE FOCUSED ULTRASONIC WITH A REDUCED POWER THRESHOLD

1. Устройство (100) для подвергания жидкой пробы (101) воздействию акустической энергии для создания кавитации в жидкой пробе, причем устройство содержит:источник (102) для испускания высокоинтенсивных ультразвуковых волн (103);при этом устройство выполнено с возможностью вмещать картридж (105), причем картридж содержит жидкую пробу и границу (106) раздела жидкость-воздух;при этом устройство выполнено с возможностью фокусировать высокоинтенсивные ультразвуковые волны так, что с помощью картриджа, вмещенного в устройство, создается фонтан (108) жидкости на границе раздела жидкость-воздух внутри картриджа.2. Устройство по п.1, дополнительно содержащее:элемент (107) зародышеобразования для уменьшения минимальной мощности, необходимой для создания кавитации в жидкой пробе; ипри этом капля (109) фонтана, возвращающаяся обратно из фонтана в жидкую пробу, является элементом зародышеобразования.3. Устройство по одному из пп.1 или 2, дополнительно содержащее:блок (110) управления для управления положением (111) фокуса высокоинтенсивных ультразвуковых волн.4. Устройство по п.1, причем устройство выполнено с возможностью лизинга клеток внутри жидкой пробы кавитацией.5. Устройство по п.1,в котором вмещение устройства обеспечивает полностью сухое соединение высокоинтенсивных ультразвуковых волн между устройством и картриджем, когда картридж вмещен в устройство.6. Устройство по п.1, дополнительно содержащее:полностью твердый соединитель (200) для по меньшей мере частичной передачи высокоинтенсивных ультразвуковых волн от источника к картриджу, когда картридж вмещен в устройство.7. Устройство по п.1, дополнительно содержащее:по меньшей мере од РОССИЙСКАЯ ФЕДЕРАЦИЯ (19) RU (11) (51) МПК G01N 33/487 (13) 2011 146 000 A (2006.01) ФЕДЕРАЛЬНАЯ СЛУЖБА ПО ИНТЕЛЛЕКТУАЛЬНОЙ СОБСТВЕННОСТИ (12) ЗАЯВКА НА ИЗОБРЕТЕНИЕ (21)(22) Заявка: 2011146000/15, 09.04.2010 (71) Заявитель(и): БИОКАРТИС СА (CH) Приоритет(ы): (30) Конвенционный приоритет: 14.04.2009 EP 09157849.2 (85) Дата начала рассмотрения ...

Methods and systems for compound management and sample preparation

The present invention provides methods and systems for automated compound management and sample preparation using acoustic energy.

The method and apparatus for separating biological entities in fluid sample

本发明涉及分离流体样品中生物实体的方法和设备。所要求保护的方法通过使用微流体装置中的声压节点基于实体的尺寸来分离生物实体。所要求保护的方法进一步用磁性标记和通过使用磁性装置分离生物实体。所要求保护的方法进一步包括微流体装置和磁性装置的组合，以从流体样品分离生物实体。

Method and device for mixing liquids

Method and device for mixing liquids

Methods and systems for modulating acoustic energy delivery

The present invention provides systems, methods, and devices for using acoustic energy. In some embodiments, a fluid bath may be provided in the system where the fluid bath quality may be monitored using acoustic energy. An assessment of fluid bath quality can be determined through a comparison that is made of an initial power signal of the acoustic energy with a reflected power signal of the acoustic energy.

System and method for ultrasonic sample preparation

A system for ultrasonic sample preparation includes a sample container having a wall defining an outer surface and an inner volume for containing a sample material, a converter which converts AC electricity to mechanical vibrations in the ultrasonic range, and an ultrasonic probe in contact with the outer surface of the sample container. The ultrasonic probe is in communication with the converter and transmits the mechanical vibrations in the ultrasonic range to the wall of the sample container and thereby to the inner volume, thereby mixing the sample material.

Concentration method, concentration apparatus and reaction method

Konzentrationsverfahren zur Aufkonzentration von Material in einer Flüssigkeit, bei dem – eine Flüssigkeit (10) mit darin befindlichem Material in ein Gefäß (12) eingebracht wird und – akustische Wellen derart in die Flüssigkeit eingestrahlt werden, dass in dem Gefäß (12) ein stationäres Strömungsmuster (20) mit Bereichen unterschiedlicher Strömungsgeschwindigkeit entsteht, wobei sich das Material (22, 24) in Bereichen minimaler Strömungsgeschwindigkeit sammelt. Concentration method for concentrating material in a liquid, in which - A liquid (10) with material therein is introduced into a vessel (12) and - Acoustic waves are radiated into the liquid such that in the vessel (12), a stationary flow pattern (20) with regions of different flow velocity is formed, wherein the material (22, 24) collects in areas of minimum flow velocity.

Stirring device, container, and analysis device with stirring device

Clinical analysis methods and systems

An improved and unique mixing technique particularly useful as part of the process for the clinical analysis of liquid biological samples is described. The sample is placed in a cuvette along with a reagent and a diluent. The top (opening) of the cuvette is squeezed (reduced in size) and an air jet is directed against the liquid surface formed in the cuvette adjacent the junction of the liquid surface with the wall of the cuvette to create a vortex to produce thorough mixing of the contents of the cuvette and substantially prevent any splashing of the liquid contents.

Chemical analysis apparatus and chemical analysis method

Provided is a chemical analysis apparatus comprising a mechanism which can efficiently agitate a substance to be agitated so that a sample and a reagent are agitated and mixed together in a shorter time with a saved consumption power, incorporating a plurality of sound sources (201) or reflecting plates (202), and a reaction container is located between one of the sound sources and another of the sound sources or one of the reflecting plates, whereby sound waves can be irradiated toward the reaction container in several directions in order to efficiently fluidize a solution in the reaction container.

Fluid-handling apparatus and methods

Fluid processing apparatus comprises a fluid-handling manifold comprising a manifold body having at least a first fluid duct and a second fluid duct. The first and second fluid ducts are in fluid communication with each other at a microfluidic junction of the fluid-handling manifold. The manifold body further comprises a transducer operative to generate ultrasonic acoustic traveling wave radiation into fluid in the microfluidic junction from an active surface toward a non-reflective boundary of the microfluidic junction. The microfluidic junction is operative to pass fluid received from the first and second duct, with micro-mixing effected by the traveling wave radiated into the junction during the fluid flow.

Sonication-assisted metal-enhanced fluorescence (samef)-based bioassays

The present invention provides for sonication-assisted metal-enhanced fluorescence, luminescence, and/or chemiluminescence assay systems using low-intensity ultrasound waves to significantly reduce the assay time by increasing the kinetic movement of molecules within the system.

Systems and methods for enhanced detection and quantification of analytes

Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.

Acoustic concentration method and device and a reaction method

The invention relates to a concentration method for concentrating materials in a liquid consisting in pouring the liquid in a material-containing receptacle and in irradiating it by acoustic waves in such a way that a stationary flow pattern with different flow rate areas is formed in the receptacle. A concentration device for carrying out the inventive method and a reaction method applying said concentration method are also disclosed.

Method for determining whether a liquid is properly stirred

A stirring determination method uses an analyzer in which a liquid contained in a vessel is stirred by an acoustic wave generated by an acoustic wave generating unit attached to the vessel, optical characteristics of a reaction solution obtained by stirring the liquid are measured, and the reaction solution is analyzed based on the optical characteristics of the reaction solution. The method includes measuring a temperature of the liquid in a position where the temperature of the liquid is different before and after the liquid is stirred; and determining whether the liquid is properly stirred based on a rate of increase in the measured temperature of the liquid.

Mixing device and analysis device having the mixing device

There are provided a mixing device (20) and an analysis device for mixing a liquid by applying a sound wave. The mixing device (20) includes a liquid holding unit (6a) for holding a liquid and a surface elastic wave element (22) arranged outside of a wall (6b) constituting the liquid holding unit for introducing a sound wave (Wa) inclined with respect to the wall (6b) and directed in two directions symmetric about a plane. The surface elastic wave element (22) generates a sound wave from a position of the symmetric plane (Ps) of a sound wave displaced from the center axis (Ac) of the liquid holding unit (6a).

Patent JPS62500322A

(57)【要約】本公報は電子出願前の出願データであるため要約のデータは記録されません。

Sonication of a medium

Sonication of a medium for example in an immunassay is provided by applying sound waves from a transducer to a vessel in which the medium is held by a sonotrode coupled between the transducer and the vessel. The sonotrode has a recess in which the vessel is held, the recess being formed by facing surfaces of a plurality of protruding portions of the sonotrode separated by slits and arranged around the recess. The sonotrode is coupled to the vessel by dry contact with the vessel without any coupling layers therebetween. The use of such a sonotrode provides the advantages of allowing effective sonication with relatively low energy loss and with a low temperature elevation.

Method and apparatus for treatment enhancement in acoustic processing of samples

Clinical analysis methods and systems

ABSTRACT An improved and unique mixing technique particularly useful as part of the process for the clinical analysis or liquid biological samples is described. The sample is placed in a cuvette along with a reagent and a diluent. The top (opening) of the cuvette is squeezed (reduced in size) and an air jet is directed against the liquid surface formed in the cuvette adjacent the junction of the liquid surface with the wall of the cuvette to create a vortex to produce thorough mixing of the contents of the cuvette and substantially prevent any splashing of the liquid contents.

Automatic chemical analysis apparatus and electrical impedance spectrometry device

撹拌動作を実行する前に撹拌機構の状態を検知することができる自動化学分析装置および電気インピーダンススペクトル測定器を提供する。　自動化学分析装置は、試薬と、分析対象であるサンプルとを反応容器１０７内に分注し、反応容器１０７へ音波を照射することにより撹拌する。自動化学分析装置は、音波を発生する圧電素子２０１と、圧電素子２０１の表面に設けられた複数の分割電極２１１と、各分割電極２１１に電圧を印加することにより、圧電素子２０１に音波を発生させる電力増幅器２０３と、各分割電極２１１に電圧を印加することにより、各分割電極２１１に係る電気インピーダンススペクトルを測定するホストコンピュータおよびインピーダンス測定回路と、を備える。

Sonication-assisted metal-enhanced fluorescence (SAMEF)-based bioassays

本发明提供了超声辅助金属增强荧光、发光和/或化学发光分析系统，所述系统使用低强度超声波，通过增加系统内分子的动力学运动，显著减少了分析时间。

Tablet dispensing

ABSTRACT A unique method and apparatus for dispensing tablets having particular application in the clinical analysis of biological samples. A series of containers are advanced past a plurality of processing stations, one of which is a tablet dispenser in turn. The processing stations are selectively activated by a controller. A sensor is associated with the tablet dispenser and is arranged to detect whether a tablet has actually entered a container. The sensor is activated only when the tablet dispenser is activated. The controller is responsive to the sensor in dependence upon the condition sensed by the sensor.

Optically measuring an immunologically active material by degree of agglutination of an antigen-antibody reaction product

There are provided a method and an apparatus for measuring an immunologically active material by physically or chemically immobilizing a material being immunologically active to a material to be measured of a specimen onto dehydrated solid fine particles, providing a desired dispersed body of said immunologically active material immobilized onto said solid fine particles in a dispersing medium, adding the specimen to said dispersed body while stirring to react the specimen with the immunologically active material, thereby causing a reaction mixture in an agglutinated state and optically measuring said agglutinated state of the reaction mixture to thereby quantitatively determine the content of the material to be measured with an improved accuracy.

Clinical analysis systems and methods

CLINICAL ANALYSIS SYSTEMS AND METHODS ABSTRACT A unique mixing technique particularly for the clinical analysis of liquid biological samples is described. The sample is placed in a cuvette along with a reagent and diluent. For mixing the sample with the reagent to facilitate reaction therewith, an air jet is directed against the liquid surface formed in the cuvette adjacent the junction of the liquid surface with the wall of the cuvette to create a vortex to produce thorough mixing of the contents of the cuvette.

Integrated chemical synthesis equipment

(57)【要約】 高圧下で連続流反応装置（１００、６０、７０）を使用して、反応混合物中の均一な温度を特徴とする、化合物合成のためのモジュール型反応装置システム（図を参照）および方法。本装置は、いくつかの一般的な成分（例えば、ポンプ、流路（８１、８２、８３、８４）、マニフォールド、制流子、および弁）を含む。組立て板（８０）上のモジュール型反応装置（１００、６０、７０）、分離装置および分析装置は、モジュール型反応装置ユニット（１００、６０、７０）が、反応ゾーン内の対流時間を最適に制御するために、100ミクロン以下の内径を有するシステムを提供する。

Fluid-handling apparatus and methods

Chemical analysis apparatus and chemical analysis method

A chemical analysis apparatus comprising reaction containers containing therein a substance to be analyzed; an agitating mechanism spaced from the substance to be analyzed and agitating said substance to be analyzed with a liquid in said reaction container; and, a measuring portion for measuring physical properties of the substance to be analyzed, said agitating mechanism having a sound supply portion supplying sound waves to the substance to be analyzed, wherein said sound supply portion comprises a mechanism changing, in time, intensity of ultrasonic waves to be irradiated so as to apply pulsation to a swirl flow in the reaction container.

Hifu induced cavitation with reduced power threshold

The invention relates to high intensity focused ultrasound devices. Furthermore the invention relates to an apparatus for irradiating a liquid sample with acoustic energy to generate cavitation in the liquid sample. The apparatus (100) comprises a source and is adapted to receive a cartridge (105) in such a way, that the apparatus focuses the HIFU waves emitted from the source onto a liquid air interface that is present within the cartridge. This focusing is performed when the cartridge is inserted into a receiving section of the apparatus.

Sample preparation apparatus

A sample preparation apparatus, comprising: an ultrasonic vibrating unit which applies ultrasonic vibration to a sample including an analyte held in a measurement sample container; a sample preparation unit which prepares a measurement sample by mixing the sample including the analyte to which the ultrasonic vibration is applied and a predetermined reagent; and a container transporting unit which transports the measurement sample container holding the sample including the analyte from the ultrasonic vibrating unit to the sample preparation unit.

Chemical reaction control device, method of manufacturing the same, and chemical reaction control method

Extraction system, and related method

Automatic preparation apparatus

An automatic preparation apparatus comprising turntable means for mounting thereon a plurality of containers including sample containers, rack means, provided at a stationary position separate from the turntable means, for mounting thereon a plurality of sample containers, robot means for transferring the sample containers between the turntable means and the rack means, and control means for performing desired treatments on the liquid in the sample containers on the turntable means.

Particle size distribution measuring apparatus

The present invention provides a particle size distribution measuring apparatus in which the sample solution circulates fluently in the flow cell which may be easily detached without spilling the sample solution, thereby permitting the flow cell to be washed easily and surely. In addition, the present invention provides a particle size distribution measuring apparatus capable of enhancing the degree of measuring accuracy irrespective of a diameter of a tube forming the flow path. The particle size distribution measuring apparatus of the present invention comprises an irradiating part which irradiates laser light to a flow cell provided in a flow path through which a sample solution flows and a detector for detecting light from the irradiating part, scattered by particles in the sample solution, wherein the flow cell is provided with two ports to be an inlet and an outlet of the sample solution on a top surface of the cell, and wherein a separating element is provided from a position between the two ports of the flow cell in a downward direction in such a way that the sample solution introduced from either one of the two ports into the flow cell is guided out of another port through the vicinity of the flow cell bottom and a lower section of the separating element has an inclined surface so as to decrease in width toward a bottom end. In another embodiment, a particle size distribution measuring apparatus is provided having a pump for circulating a sample solution and a flow cell in the flow path through which the sample solution circulates and having an irradiating part which irradiates laser light to the flow cell and a detector for detecting light from the irradiating part, scattered by particles in the sample solution contained in the flow cell, wherein a sample solution circulation system is constructed so as to reverse the flow direction of the sample solution which flows through the flow cell prior to measurement of the particle size distribution performed by ...

Sample preparation apparatus

A sample preparation apparatus, comprising: an ultrasonic vibrating unit which applies ultrasonic vibration to a sample including an analyte held in a measurement sample container; a sample preparation unit which prepares a measurement sample by mixing the sample including the analyte to which the ultrasonic vibration is applied and a predetermined reagent; and a container transporting unit which transports the measurement sample container holding the sample including the analyte from the ultrasonic vibrating unit to the sample preparation unit.

Microfabricated reactor

An integrated microfabricated instrument for manipulation, reaction and detection of microliter to picoliter samples. The instrument is suited for biochemical reactions, particularly DNA-based reactions such as the polymerase chain reaction, that require thermal cycling since the inherently small size of the instrument facilitates rapid cycle times. The integrated nature of the instrument provides accurate, contamination-free processing. The instrument may include reagent reservoirs, agitators and mixers, heaters, pumps, and optical or electromechanical sensors. Ultrasonic Lamb-wave devices may be used as sensors, pumps and agitators.

Apparatus and methods for controlling sonic treatment

Apparatus and methods are disclosed for treating a sample by selectively controlling sonic energy and/or selectively controlling the location of the sample relative to the sonic energy.

Sonication of a medium

Sonication of a medium for example in an immunassay is provided by applying sound waves from a transducer to a vessel in which the medium is held by a sonotrode coupled between the transducer and the vessel. The sonotrode has a recess in which the vessel is held, the recess being formed by facing surfaces of a plurality of protruding portions of the sonotrode separated by slits and arranged around the recess. The sonotrode is coupled to the vessel by dry contact with the vessel without any coupling layers therebetween. The use of such a sonotrode provides the advantages of allowing effective sonication with relatively low energy loss and with a low temperature elevation.

Devices, systems and methods for sample preparation

Devices, systems and methods including a sonicator for sample preparation are provided. A sonicator may be used to mix, resuspend, aerosolize, disperse, disintegrate, or de-gas a solution. A sonicator may be used to disrupt a cell, such as a pathogen cell in a sample. Sample preparation may include exposing pathogen-identifying material by sonication to detect, identify, or measure pathogens. A sonicator may transfer ultrasonic energy to the sample solution by contacting its tip to an exterior wall of a vessel containing the sample. Multipurpose devices including a sonicator also include further components for additional actions and assays. Devices, and systems comprising such devices, may communicate with a laboratory or other devices in a system for sample assay and analysis. Methods utilizing such devices and systems are provided. The improved sample preparation devices, systems and methods are useful for analyzing samples, e.g. for diagnosing patients suffering from infection by pathogens.

Sample injection apparatus and liquid chromatography apparatus having the sample injection apparatus

A sample injection apparatus and a liquid chromatography apparatus including the sample injection apparatus are provided. The sample injection apparatus includes a sampling vessel into which a sample is supplied, a sampling needle for aspirating and ejecting the sample, a cleaning part into which a cleaning liquid for cleaning at least the sampling needle is supplied, a sample injection part for injecting the sample ejected from the sampling needle into a moving liquid, and a needle transfer part for transferring the sampling needle among the sampling vessel, the cleaning part and the sample injection part, wherein the cleaning part includes an ultrasonic vibrator for generating an ultrasonic wave in the cleaning liquid.

ALUMINUM OR ALUMINUM ALLOY TARGET

Extraction system

An extraction system for distributing a test product (20) in an extraction fluid, the system including a flow cell (2) and a pump (16). The flow cell has walls defining an internal passageway (4) having an extraction region (10) for holding the test product and also an upstream end (12) and a downstream end (14) between which the extraction region extends. The internal cross-sectional area of the upstream end is larger than the internal cross-sectional area of the downstream end the and extraction region tapers from the upstream end to the downstream end. The pump has an inlet in fluid communication with the downstream end and an outlet in fluid communication with the upstream end. The pump re-circulates extraction fluid from the downstream end back to the upstream end so as to progressively erode the test product and distribute the test product in the extraction fluid.

Liquid control apparatus

A liquid control apparatus is used for controlling the movement of liquid in a microfluidic device having a flow channel for holding liquid. The apparatus comprises a vibration wave generating section for generating a vibration wave to be applied to the microfluidic device and a signal supplying section for supplying drive signals to the vibration wave generating section so as to oscillate in an oscillation mode selected from a transfer mode for moving liquid in a predetermined direction, a stop mode for stopping the movement of liquid, a mixing and/or agitation mode for mixing and/or agitating liquid and a localization mode for localizing a predetermined substance in liquid.

Automated dispersive liquid-liquid microextraction technique for the analysis of N-nitrosamines in water

An automated dispersive liquid-liquid microextraction method of detecting and quanta N-nitrosamines in an aqueous sample. The method includes (a) extracting an aqueous solution containing the N-nitrosamines by mixing an extraction solvent and a dispersive solvent with the aqueous solution, such that the N-nitrosamines, or a portion thereof, re-distribute from the aqueous solution to the extraction solvent, (b) permitting the resulting mixture in (a) to form a two-phase mixture containing an aqueous phase comprising containing the aqueous solution with reduced amounts of the N-nitrosamines and an organic phase containing the extraction solvent with the N-nitrosamines extracted from the aqueous solution, (c) injecting the organic phase, or a portion thereof, into an injection port of a gas chromatograph coupled with at least one mass spectrometer, and (d) analyzing the N-nitrosamines by gas chromatography and mass spectrometry to detect and quantify the concentration of the N-nitrosamines in the aqueous solution.

Automatic analyzer and analysis method

Ultrasonic transducing probe with liquid flow-through capability and related automated workstation and methods of using same

A hollow probe cooperates with an ultrasonic transducing device designed with liquid flow-through capability. The probe and transducing device are combined into a probe assembly, which can be integrated into an automated liquid handling workstation. As a functional component of the workstation, the probe can be connected to and manipulated by a robotic arm of the workstation, and thus programmed to move in three-dimensional space to and from various locations of the sampling apparatus. In particular, the probe can be inserted into the individual wells or test tubes of a plate or rack utilized to contain sample substances. The probe can be used to conduct a variety of liquid handling tasks and additionally can be used to ultrasonically excite sample substances contained in the individual wells of the plate, thereby improving dilution of such sample substances and increasing throughput of any given sample preparation procedure. A liquid level detection device can be connected to the probe assembly.

Integrated chemical synthesizers

A modular reactor system (see figure) and method for synthesizing chemical compounds characterized by a uniform temperature throughout the reaction mixture by use of a continuous flow reactor (100, 60, 70) under high pressure. The apparatus includes a number of generic components such as pumps, flow channels (81, 82, 83, 84), manifolds, flow restrictors, and valves. Modular reactors (100, 60, 70), separator and analyzers on an assembly board (80) provide a system where a modular reactor unit (100, 60, 70) has an internal diameter of up to 100 micrometers to optimize control of residence time within a reaction zone.

Phase-modulated standing wave mixing apparatus and methods

Disclosed are mixing apparatus adapted to provide mixing of components in an automated analyzer. The mixing apparatus includes a reservoir configured to contain a coupling liquid, a transducer configured to be driven at a frequency and communicate with the coupling liquid, and a signal generation unit configured to provide a phase modulatable drive signal to the transducer. In some embodiments, improved patient sample and reagent mixing may be provided. Systems and methods are provided, as are other aspects.

Controlling sonic treatment

An apparatus 100 for processing a sample 800 using acoustic energy, the apparatus comprises: an acoustic energy source 230 for emitting acoustic energy, wherein the acoustic energy source generates a wavetrain substantially converging in a focal zone having a diameter less than about 2 centimeters; a medium 600 for coupling the wavetrain to the sample 800; a reaction vessel 502, 504, 506 for holding the sample 800; and a processor for controlling at least one of the acoustic energy source 230 and a location of the reaction vessel 502, 504, 506 relative to the focal zone, such that the sample 800 is selectively exposed to acoustic energy to produce a desired result.

Phase-modulated standing wave mixing device and method

公开了一种混合装置，其适配成提供自动化分析仪中的组分的混合。混合装置包括配置成包含耦合液体的贮液器、配置成以一频率驱动并且与耦合液体通信的换能器、以及配置成向换能器提供相位可调制驱动信号的信号生成单元。在一些实施例中，可以提供改进的患者样本和试剂混合。作为其它方面提供了系统和方法。

Sample analyzer and sample analyzing method

一种样本分析仪和样本分析方法，样本分析仪包括混匀位、混匀装置和控制器，混匀位用于放置容纳杯，混匀装置用于对放置在混匀位的容纳杯中的待混液体执行混匀操作，混匀装置包括至少两个混匀机构，控制器用于控制一个或至少两个混匀机构对容纳杯中的待混液体执行混匀操作，当至少两个混匀机构对容纳杯中的待混液体执行混匀操作时，各混匀机构对应执行的混匀模式各不相同。由于样本分析仪上的混匀装置包括至少两个混匀机构，多个混匀机构用于执行不同的混匀模式，使得针对不同的测试项目能够匹配对应的一个或至少两个混匀机构对待混匀液体执行混匀操作，以实现有效混匀，进而能够保证不同测试项目检测的准确性。

Extraction system

An extraction system for distributing a test product (20) in an extraction fluid, the system including a flow cell (2) and a pump (16). The flow cell has walls defining an internal passageway (4) having an extraction region (10) for holding the test product and also an upstream end (12) and a downstream end (14) between which the extraction region extends. The internal cross-sectional area of the upstream end is larger than the internal cross-sectional area of the downstream end the and extraction region tapers from the upstream end to the downstream end. The pump has an inlet in fluid communication with the downstream end and an outlet in fluid communication with the upstream end. The pump re-circulates extraction fluid from the downstream end back to the upstream end so as to progressively erode the test product and distribute the test product in the extraction fluid.

Cartridges, analyzers, and systems for hemolysing and analyzing samples using oximetry

A cartridge, analyzer for use therewith, and system including the cartridge and analyzer. The cartridge is configured to receive a biological fluid to be analyzed and includes a plate defining a main channel, a hemolysis chamber, and an oximetry chamber consecutively interconnected with one another. The analyzer is configured to perform analysis of sample disposed within the main channel, hemolyze sample disposed within the hemolysis chamber, and perform oximetry on sample disposed within the oximetry chamber. The cartridge and analyzer may further include alignment and clamping structures for maintain the cartridge in fixed position and alignment during testing or may further include cooperating features for moving the cartridge relative to the analyzer into a testing position or between various different testing positions.

Ultrasonic transducing probe with liquid flow-through capability and related automated workstation and methods of using same

A hollow probe cooperates with an ultrasonic transducing device designed with liquid flow-through capability. The probe and transducing device are combined into a probe assembly, which can be integrated into an automated liquid handling workstation. As a functional component of the workstation, the probe can be connected to and manipulated by a robotic arm of the workstation, and thus programmed to move in three-dimensional space to and from various locations of the sampling apparatus. In particular, the probe can be inserted into the individual wells or test tubes of a plate or rack utilized to contain sample substances. The probe can be used to conduct a variety of liquid handling tasks and additionally can be used to ultrasonically excite sample substances contained in the individual wells of the plate, thereby improving dilution of such sample substances and increasing throughput of any given sample preparation procedure. A liquid level detection device can be connected to the probe assembly.

Integrated chemical synthesizers

A modular reactor system (see figure) and method for synthesizing chemical c ompounds characterized by a uniform temperature throughout the reaction mixture by use of a continuous flow reactor (100, 60 , 70) under high pressure. The apparatus includes a numb er of generic components such as pumps, flow channels (81, 82, 83, 84), manifolds, flow restrictors, and valves. Modular reactors (100, 60 , 70), separator and analyzers on an assembly board (80) provide a system where a m odular reactor unit (100, 60, 70) has an internal diamet er of up to 100 micrometers to optimize control of residence time within a reactio n zone.