29-08-2019 дата публикации
Номер: US20190264169A1
Принадлежит:
The present disclosure provides compositions and methods for using recombinant Cmetabolizing microorganisms capable of metabolizing sulfur containing compounds and other contaminants to biologically convert sour or acidic natural gas into high-value molecules, and to allow recovery of stranded oil. 1125.-. (canceled)126. A recombinant methanotrophic or methylotrophic bacterium , comprising: [{'sup': '+', '(i) a hydrogen sulfide:NADP oxidoreductase, hydrogen sulfide:ferredoxin oxidoreductase, sulfide: flavocytochrome-c oxidoreductase, sulfide: quinone oxidoreductase, sulfur dioxygenase, sulfite oxidase, or any combination thereof;'}, {'sup': '+', '(ii) a hydrogen sulfide:NADP oxidoreductase, sulfite oxidase, or both;'}, '(iii) a hydrogen sulfide:ferredoxin oxidoreductase, sulfite oxidase, or both;', '(iv) a sulfide:flavocytochrome-c oxidoreductase, sulfite oxidase, or both;', '(v) a sulfide:quinone oxidoreductase, sulfite oxidase, or both; and', {'sup': '+', '(vi) a hydrogen sulfide:NADP oxidoreductase, hydrogen sulfide:ferredoxin oxidoreductase, sulfide: flavocytochrome-c oxidoreductase, or sulfide: quinone oxidoreductase, wherein endogenous sulfite oxidase activity of the bacterium is increased; and'}], '(a) a first heterologous nucleic acid molecule encoding a polypeptide capable of metabolizing an S substrate selected from (i) the cysteine biosynthesis pathway comprises a cysteine synthase;', '(ii) the homocysteine biosynthesis pathway comprises an O-acetylhomoserine sulfhydrylase; and', '(iii) the methionine biosynthesis pathway comprises an O-acetylhomoserine sulfhydrylase and a methionine synthase or a homocysteine methyltransferase;, '(b) a second heterologous nucleic acid molecule encoding a cysteine biosynthesis pathway, homocysteine biosynthesis pathway, or a methionine biosynthesis pathway, wherein{'sub': '1', 'wherein the recombinant methanotrophic or methylotrophic bacterium is capable of converting a feedstock comprising the S substrate and a ...
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