17-10-2019 дата публикации
Номер: WO2019196194A1
Принадлежит:
A kit for detecting a human regulatory T cell subtype and a detection method thereof, belonging to the technical field of cell subtype detection. The kit comprises the following components: a blood diluent, a mononuclear cell separation solution, a cell culture solution, a lymphocyte activation solution, a dead cell removal dye, an FcR blocking agent, a fluorescence labeled antibody resisting a human cell surface labeling molecule, a fluorescence labeled antibody resisting a human intracellular molecule, a PBS buffer solution, lipopolysaccharide, a washing buffer solution, a cell dyeing buffer solution, a cell fixing solution, and a permeable membrane lotion. During detection, a mononuclear cell in whole blood is first separated; then, a mononuclear cell of human peripheral blood is stimulated; and finally, a fluorescent antibody is stained, so that the regulatory T cell subtype can be detected. By use of the kit and the detection method, 2-6 regulatory T cell subtypes can be simply and ...
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