钱包（WBL2383）

钱包（WBL2384）

RAILWAY STATION GATE WITH TICKET PREPARING FUNCTION AND METHOD FOR PREPARING TICKET FOR RAILWAY STATION GATE

A railway station gate with a ticket preparing function and a method for preparing a ticket for the railway station gate comprise a magnetic paper ticket checking device (1) and an identity checking device. The magnetic paper ticket checking device (1) comprises a first conveying channel (11), a magnetism reading module (12), a first magnetism writing module (13), a first magnetism checking module (14), and a first printing module (15). The railway station gate and the method further comprise a magnetic paper ticket preparing device (2) for preparing a ticket for a passenger who uses the identity checking device to perform checking and who does not yet have a ticket. The magnetic paper ticket preparing device (2) comprises a ticket source mechanism, a second conveying channel (21), and a ticket cutting module (22). An outlet of the second conveying channel (21) is connected to the first conveying channel (11) at a front side of any one of an inlet of the magnetic paper ticket checking device ...

钱包（WBL2386）

钱包（WBL2385）

钱包（WBL2387）

Preparation method of size-controllable chiral selenium nanoparticles

The invention discloses a preparation method of size-controllable chiral selenium nanoparticles, which comprises the following steps: (1) mixing hexadecyl trimethyl ammonium bromide, selenium dioxide and chiral amino acid in an aqueous solution, and stirring for reaction to obtain a mixed solution; and (2) adding water and sodium borohydride into the mixed solution, continuously reacting, and centrifuging after the reaction is finished to obtain the chiral selenium nanoparticles. According to the method, the size of the chiral selenium nanoparticles can be regulated and controlled by controlling the adding amount of the water in the step (2), and controllable synthesis of the chiral nanoparticles between 100 nm and 500 nm is achieved. The preparation method is simple to operate, mild in reaction condition and short in reaction time, the product is easy to separate, and the prepared chiral selenium nanoparticles are good in dispersity and uniformity, have high optical activity, can be used ...

METHOD FOR SIMULTANEOUSLY DETECTING TWO TYPES OF MIRNAS ON THE BASIS OF FRET EFFECTS

A method for simultaneously detecting two types of miRNAs on the basis of FRET effects, relating to the field of material chemistry. According to the method, a gold rod nucleus-upconversion satellite-shaped structure assembly is synthesized, a standard curve is built, a golden rod nucleus-upconversion satellite-shaped nanocrystal assembly with a uniform structure and good biocompatibility is finally prepared, and a platform capable of implementing quantitative detection of two types of intracellular miRNAs on the basis of FRET effects is provided.

HYBRIDOMA CELL STRAIN C1 FOR SECRETING ANTI-PAROMOMYCIN MONOCLONAL ANTIBODY AND USE THEREOF

Disclosed are a hybridoma cell strain C1 for secreting an anti-paromomycin monoclonal antibody and the use thereof, belonging to the technical field of immunochemistry. A murine monoclonal cell strain C1 has been prepared through the conventional cell fusion technologies and deposited in the China General Microbiological Culture Collection Centre, and the deposit number is CGMCC No. 12025. The monoclonal antibody secreted by the cell strain is determined by the indirect competitive enzyme-linked immunosorbent assay, and the IC50 to paromomycin and neomycin is 0.61 and 2.43 μg/L respectively. The range of the recovery rate of paromomycin and neomycin in animal foodstuff is 64.56-105.85% and 54.08-100.55%. The hybridoma cell strain C1 provides a raw material for the immunological detection of the paromomycin residue in animal foodstuff, and has a practical application value.

FLUNIXIN MEGLUMINE MONOCLONAL ANTIBODY HYBRIDOMA CELL STRAIN YY AND APPLICATION THEREOF

Provided is a flunixin meglumine monoclonal antibody hybridoma cell strain YY, which has been collected in the China General Microbiological Culture Collection Center (referred to as CGMCC), with the accession number being CGMCC No.13096. Also provided are a monoclonal antibody secreted by the hybridoma cell strain YY, and an application of the monoclonal antibody. Also provided are a method for preparing a flunixin meglumine antigen, the antigen prepared by the method, and an application of the antigen.

PREPARATION METHOD FOR SATELLITE-SHAPED NANOASSEMBLY USED FOR INTRACELLULAR CANCER MARKER DUAL DETECTION, AND APPLICATION

Provided are a preparation method for a satellite-shaped nanoassembly used for intracellular cancer marker dual detection, and an application. The present invention comprises: one, satellite-shaped nanoassembly preparation: synthesising a gold nanorod, preparing a 20nm upconverting nanoparticle, assembling a gold rod dimer, coupling a Raman signal molecule DTTC on the gold rod dimer, and assembling a gold rod dimer core–upconverting satellite-shaped structure; two, intracellular cancer marker dual detection: modifying the gold rod dimer core–upconverting satellite-shaped nanostructure assembly with a membrane-penetrating peptide, intracellular detection by the gold rod dimer core–upconverting satellite-shaped nanostructure assembly, characterising the intracellular detection by the gold rod dimer core–upconverting satellite-shaped nanostructure assembly, and establishing a standard curve.

Application of chiral nanometer material based on microglial cell inflammatory aging regulation in preparation of medicine for treating Alzheimer's disease

The invention discloses an application of a chiral nano material based on microglial cell inflammatory aging regulation in preparation of a medicine for treating Alzheimer's disease, a microminiature chiral gold nano particle is prepared, and the nano particle is coupled with an antibody of a targeted inflammasome NLRP3 to form a chiral nano assembly. The ultra-small chiral gold nanoparticles prepared by the method have the capability of selectively inhibiting beta amyloid protein aggregation. According to the chiral nano assembly prepared by the preparation method disclosed by the invention, the restriction of the blood brain barrier (BBB) in antibody targeted delivery is overcome by utilizing efficient combination of the chiral gold nanoparticles and the SLC3A2, and the antibody is effectively delivered into microglia cells in the brain. The antibody targets NLRP3 and degrades NLRP3 in microglial cells in a TRIM21-dependent proteasome degradation way, inflammatory aging of the microglial ...

METHOD FOR SYNTHESIZING CHIRAL QUANTUM ROD BASED ON CIRCULARLY POLARIZED LIGHT

A method for synthesizing a chiral quantum rod based on circularly polarized light, pertaining to the technical field of analytical chemistry. The present invention is a preparation method for transforming chiral quantum dots into chiral quantum rods by irradiation of circularly polarized light. The present invention mainly comprises two parts: the preparation of chiral quantum dots; and the synthesis of chiral quantum rods on the basis of the chiral quantum dots. The present invention overcomes the shortcomings of the difficulty of aqueous-phase synthesis of conventional quantum rods and the difficulty of controlling the morphology of the quantum rods, and resolves the problems of fluorescence quenching and property instability resulting from a complex transition from an organic phase to an aqueous phase. Instead, the present invention directly transforms quantum dots into quantum rods in an aqueous solution by using circularly polarized light, facilitating a wide range of application ...

Preparation method of dexmethylphenidate hydrochloride sustained-release capsule

The invention provides a dexmethylphenidate hydrochloride sustained-release capsule, the capsule takes a dexmethylphenidate hydrochloride sustained-release micro-tablet as a drug carrying unit, the micro-tablet comprises a quick-release layer and a sustained-release layer, and the quick-release layer comprises dexmethylphenidate hydrochloride, a filler I, a disintegrating agent and pharmaceutically acceptable auxiliary materials; the sustained-release layer comprises dexmethylphenidate hydrochloride, a filler II, a sustained-release material and pharmaceutically acceptable auxiliary materials, the average diameter of the micro-tablet is 3-10mm, the height of the quick-release layer is 1-4mm, the height of the sustained-release layer is 2-6mm, the particle size distribution of dexmethylphenidate hydrochloride is that D50 is more than or equal to 50 microns and less than or equal to 100 microns, D90 is more than or equal to 110 microns and less than or equal to 180 microns, and the particle ...

MONOCLONAL CELL LINE C4 CAPABLE OF SECRETING MONOCLONAL ANTIBODY RECOGNIZING METHYLENE BLUE AND APPLICATION THEREOF

The invention discloses a monoclonal cell line C4 capable of secreting a monoclonal antibody recognizing methylene blue and an application thereof in the technical field of immunochemistry. The monoclonal cell line C4 provided by the invention has been deposited with the general microbial center of the China General Microbial Culture Collection Committee under Accession Number CGMCC No.12026. The monoclonal antibody secreted by the monoclonal cell line C4 can specifically recognize methylene blue with good specificity and high sensitivity, and IC50 is 21.13ng/ml. The monoclonal antibody can be used to establish immune detection technology for methylene blue.

A LINCOSAMIDE UNIVERSAL MONOCLONAL ANTIBODY HYBRIDOMA CELL STRAIN AND APPLICATION THEREOF

Disclosed is a lincosamide universal monoclonal antibody hybridoma cell strain and application thereof, belonging to the technical field of food safety immunological detection. The invention adopts chlorine-substituted clindamycin derivative as a hapten, and the hapten is coupled with bovine serum albumin (BSA) by an activated ester method to obtain an immune antigen. The immune antigen is uniformly mixed with Freund's adjuvant and used to immunize a BALB/c mouse by subcutaneous injection. The clindamycin is coupled with ovalbumin (OVA) by a carbonyldiimidazole (CDI) method as a coating antigen for mouse serum and cell supernatant detection. The spleen cells of the immunized mice are fused with mouse myeloma cells by a PEG method, and subjected to indirect ELISA, indirect competitive ELISA screening and three subclones to obtain a population-selective hybridoma cell strain. The cell strain provided by the invention has excellent inhibitory effect on clindamycin, lincomycin and pirlimycin ...

HYBRIDOMA CELL LINE SECRETING MONOCLONAL ANTIBODY AGAINST THIAMETHOXAM AND USE THEREOF

A hybridoma cell line secreting a monoclonal antibody against thiamethoxam, wherein the deposit number of the hybridoma cell line is: CGMCC No.14699. The cell line can be obtained by the steps of: immunizing BALB/c mice with a complete Freund's adjuvant for the first time, with an incomplete Freund's adjuvant for three booster immunizations, and with the thiamethoxam complete antigen without an adjuvant for one impact immunization to immunize the BALB/c mice; fusing the immunized mouse spleen cells with high titer and low IC50 with mouse myeloma cells by a PEG method, and subjecting same to the indirect competitive ELISA screening and three subclonings. The monoclonal antibody secreted by the cell line can be used for the detection of thiamethoxam residues in food.

PREPARATION METHOD OF SALMONELLA-DETECTING COLLOIDAL GOLD TEST STRIP FOR FOOD BASED ON SALMONELLA CORE POLYSACCHARIDE MONOCLONAL ANTIBODY

The present invention relates to the field of immunological assays, and provides a preparation method of a Salmonella-detecting colloidal gold test strip for food based on a Salmonella core polysaccharide monoclonal antibody SQX6D8. A conjugate of Salmonella typhimurium lipopolysaccharide (LPS) synthesized by a sodium periodate-based method with bovine serum albumin (BSA) is used as a coating antigen of a test line (T line) of the colloidal gold test strip. The Salmonella core polysaccharide monoclonal antibody SQX6D8 is used as a gold-labeled antibody. In contrast to the sandwich principle employed by conventional pathogenic bacterium colloidal gold test strips, the Salmonella colloidal gold test strip method of the present invention employs a concept of indirect competition to perform an assay; in addition, a monoclonal antibody specific to a Salmonella core polysaccharide is used to ensure that the method is cross-reactive with a bacterium in the Salmonella genus and is not cross-reactive ...

IPRODIONE MONOCLONAL ANTIBODY HYBRIDOMA CELL STRAIN ZXL-2 AND APPLICATION THEREOF

Provided is an iprodione monoclonal antibody hybridoma cell strain ZXL-2, which has been collected in the China General Microbiological Culture Collection Center, with the accession number being CGMCC No. 13093. A monoclonal antibody secreted by the hybridoma cell strain ZXL-2 has relatively good specificity and detection sensitivity (IC50 value is 5 ng/mL) to iprodione. The hybridoma cell strain ZXL-2 is used for immunodetection of an iprodione residue in food.

IMAGE QUALITY COMPENSATION DEVICE AND METHOD, AND OPTICAL SYSTEM

An image quality compensation device, comprising a lens tube (8), a force application mechanism (10), and a rotating mechanism (9), a lens (7) being contained within the lens tube; the force application mechanism may apply pressure on the lens tube to deform the lens in the lens tube, and the lens tube may rotate following the rotation of the rotating mechanism. Further provided are an image quality compensation method and an optical system comprising an image quality compensation device. The image quality compensation device and method and the optical system may compensate image quality by means of the rotation and deformation of a lens, and may compensate a plurality of image qualities, thus reducing the difficulty of image quality compensation while reducing costs.

HYBRIDOMA CELL LINE NAN-1 WHICH SECRETES SULFONAMIDE ANTIBIOTIC-RESISTANT MONOCLONAL ANTIBODY, AND APPLICATION OF HYBRIDOMA CELL LINE

A hybridoma cell line NaN-1 which secretes a sulfonamide antibiotic-resistant monoclonal antibody, and an application of the hybridoma cell line, belonging to the field of food safety immunoassays. The antibody secreted by the hybridoma cell line NaN-1 is capable of identifying twenty-five sulfonamide antibiotics. The hybridoma cell line has been stored in the China General Microbiological Culture Collection Center (CGMCC), the accession number being CGMCC No. 12028. The monoclonal antibody secreted by the cell line NaN-1 is capable of identifying twenty-five sulfonamide antibiotics and has good detection sensitivity. The IC50 for sulfadiazine (SD), sulfamerazine (SMR), sulfadimethoxine (SDM) and sulfathiazole (STZ) is respectively: 4.51, 1.80, 1.98 and 0.53 μg/L. The present invention can be used for sulfonamide antibiotic multi-residue detection within the field of food safety.

Double-S-type resident electricity consumption temperature prediction method

The invention relates to a double-S-type resident electricity consumption temperature prediction method. The method comprises the following steps of 1, solving parameters of a model expression; step 2, temperature treatment; step 3, inflection point processing; 4, temperature transition period treatment; 5, model rolling correction is carried out; the method has the advantages that the segmentation points are unique, breakpoints do not exist, the model fitness is high, and high prediction accuracy can be guaranteed without real-time adjustment.

GOLD-GRAPHENE NANOASSEMBLY-BASED MUCIN-1 CHIRALITY SIGNAL ULTRASENSITIVE DETECTION METHOD

A gold-graphene nanoassembly-based mucin-1 chirality signal ultrasensitive detection method, belonging to the technical field of material chemistry. The present invention comprises gold nanoparticle synthesis, graphene nanomaterial preparation, gold nanoparticle and mucin-1 aptamer coupling, graphene and mucin-1 aptamer partially complementary sequence coupling, gold-graphene nanostructure assembly, and TEM and chirality signal characterisation and determination. A relationship between the concentration of mucin-1 and a chirality signal of an assembly is established on the basis that different concentrations of mucin-1 relate to different dissociation degrees of the assembly and thus affect the strength of the chirality signal, and said relationship is used to implement the quantitative detection of mucin-1. The present invention provides a method for the ultrasensitive detection of a mucin-1 amount via a circular dichroism signal, and prepares a gold-graphene self-assembly nanostructure ...

Chiral selenium nano-film with photoresponse, preparation method of chiral selenium nano-film and application of chiral selenium nano-film in detection of L-kynurenine

The invention provides a chiral selenium nano-film with photoresponse, a preparation method of the chiral selenium nano-film and application of the chiral selenium nano-film in detection of L-kynurenine, and belongs to the field of two-dimensional nano-film biotechnology detection. A cysteine chiral ligand is introduced in the synthesis process to obtain chiral selenium nanoparticles, and the chiral selenium nanoparticles are subjected to liquid-liquid interface self-assembly to prepare the single-layer uniform chiral selenium nano-film with photoresponse. The prepared chiral selenium nano-film with photoresponse can be used for detecting the content of L-kynurenine in a human body. Under the illumination condition, the chiral selenium nano-film obtains photoresponse current, and after L-kynurenine is added, a photocurrent signal can be weakened, so that a standard curve of the photocurrent signal and the concentration of the L-kynurenine is established, and the content of the L-kynurenine ...

METHOD FOR DOUBLE-SIGNAL IN-SITU DETECTION OF INTRACELLULAR MICRORNA BASED ON GOLD-UPCONVERSION NANOPARTICLE TETRAHEDRON

The invention provides a method for double-signal in-situ detection of intracellular microRNA based on a gold-upconversion nanoparticle tetrahedron. The method includes: preparing a configuration of a nucleic acid for use with a detection probe, preparing a gold-upconversion nanoparticle tetrahedron detection probe, functionalization of the tetrahedron, and detecting an intracellular microRNA.

INTRACELLULAR ATP CIRCULAR DICHROISM SPECTRUM REAL-TIME DETECTION METHOD

Disclosed is an intracellular ATP circular dichroism spectrum real-time detection method, belonging to the technical field of material chemistry. The method comprises constructing a circular dichroism size gold dimer sensor from an ATP aptamer sequence, constructing a circular dichroism size gold dimer sensor from ATP mismatch sequence DNA, modifying the two size gold dimers with a membrane-penetrating peptide, and when circular dichroism signals of the two size gold dimer sensors change with time within a cell, implementing intracellular ATP real-time detection and establishing a standard curve on the basis of the circular dichroism signals of the size gold dimers. Also disclosed is a method for the real-time detection of an ATP amount within a cell via circular dichroism signals, and a gold nanoparticle dimer having high circular dichroism performance and good biocompatibility.

Chiral zinc oxide and synthesis method thereof

The invention provides chiral zinc oxide and a synthesis method thereof. According to the chiral zinc oxide, under the alkaline condition, a plurality of chiral amino acids are used as chiral inducers, and chiral units are formed through interaction of chiral ligands and zinc ions; by regulating and controlling the dielectric constant of the solvent, the chiral units are induced to form micro-nano-scale chiral zinc oxide through intermolecular interaction accumulation; by adjusting the type of the chiral ligand, the dielectric constant of the solution and the kinetic and thermodynamic parameters of the reaction, the chiral zinc oxide with various morphologies can be obtained. Chiral zinc oxide with different morphologies and different scales shows strong circular dichroism absorption signals. The synthesis method of the chiral zinc oxide provided by the invention comprises synthesis and characterization of the chiral zinc oxide. Zinc oxide micro-nano structures with various scales and various ...

Pair of hybridoma cell strains capable of secreting anti-prolactin monoclonal antibody, anti-prolactin monoclonal antibody and application of anti-prolactin monoclonal antibody

The invention relates to a pair of hybridoma cell strains capable of secreting a prolactin monoclonal antibody, and belongs to the field of immunology and in vitro diagnosis. The preparation method comprises the following steps: immunizing a mouse by using a recombinant PRL protein, screening positive clones by using an enzyme-linked immunoassay after fusion, cloning the obtained positive clones for at least four times by using a limited dilution method until monoclone is obtained, and screening a cell strain with high affinity from the obtained monoclone; the antibody secreted by the hybridoma cell strain is further paired by using a colloidal gold immunochromatography method, and finally two hybridoma cell strains capable of stably secreting the antibody are obtained. The anti-PRL antibody provided by the invention has excellent performance in all aspects, especially has high affinity, and thus is suitable for being used as a core raw material of a PRL immunodetection reagent.

Chiral titanium dioxide as well as preparation method and application thereof

The invention relates to chiral titanium dioxide as well as a preparation method and application thereof, and belongs to the technical field of chiral materials. The preparation method of the chiral titanium dioxide comprises the following steps: carrying out secondary induction on a titanium source and cysteine in a solvent under an alkaline condition, and reacting to obtain the chiral titanium dioxide. According to the preparation method disclosed by the invention, the chiral titanium dioxide with the anatase crystal form is found for the first time. According to the preparation method, the research on the interaction between the small molecular chiral ligand and the inorganic material is of great significance for developing more chiral materials and understanding the interaction between the chiral materials. According to the chiral titanium dioxide disclosed by the invention, the selection of cosmetic markets is expanded, and a method basis is provided for research of chiral materials ...

Flower-shaped chiral aluminum hydroxide nano material and preparation method thereof

The invention belongs to the technical field of inorganic chemistry, and particularly relates to a flower-shaped chiral aluminum hydroxide nano material and a preparation method thereof. According to the invention, a chiral ligand is added into an aluminum hydroxide precipitate forming process, and a solvent dispersion method is used for inducing to obtain a target product, namely the flower-shaped chiral aluminum hydroxide. The chiral aluminum hydroxide nano material is of a flower-shaped structure formed by chirality surrounding and stacking of lamellae. The flower-like chiral aluminum hydroxide nano material is high in safety and good in dispersity in water, has CD activity in a near-infrared region, and can be used for identification and detection of polarized light. Meanwhile, the material has an obvious chiral configuration, is more suitable for an organism environment, can be used as an adjuvant to prolong the action time of an antigen, and can also promote response of local macrophages ...

Anti-quinidine monoclonal antibody, hybridoma cell strain and application

The invention provides an anti-quinidine monoclonal antibody, a hybridoma cell strain and application, and belongs to the field of food safety immunodetection. The preservation number of the hybridoma cell strain is CGMCC (China General Microbiological Culture Collection Center) The monoclonal antibody secreted by the cell strain has good specificity and detection sensitivity (IC50 value is 0.5 ng/mL) to quinidine and can be used for immunoassay detection, the result of the invention can be used for preparing a quinidine immunodetection kit and a colloidal gold test strip, and a powerful detection method and means are provided for detection of quinidine residual quantity.

Hybridoma cell strain secreting torasemide monoclonal antibody and application thereof

The invention discloses a hybridoma cell strain capable of secreting a torasemide monoclonal antibody and application of the hybridoma cell strain, and belongs to the field of food safety immunodetection. According to the invention, a torasemide hapten is synthesized, a torasemide complete antigen is prepared, the torasemide complete antigen and an equal amount of a Freund's adjuvant are mixed and emulsified completely, a BALB/c mouse is immunized through back subcutaneous injection, the Freund's complete adjuvant is used for first immunization, the Freund's incomplete adjuvant is used for multiple booster immunization, and the torasemide complete antigen is used for sprint immunization for the last time. The hybridoma cell strain is obtained by taking high-titer and low-IC50 mouse splenocytes, fusing the high-titer and low-IC50 mouse splenocytes with myeloma cells through a PEG (Polyethylene Glycol) method and carrying out screening and three times of subcloning through an indirect competitive ...

Chiral iron-based superparticle nano material as well as preparation method and application thereof

The invention relates to a chiral iron-based superparticle nano material as well as a preparation method and application thereof, and belongs to the technical field of inorganic nano material modification. The chiral iron-based superparticle nano material is prepared from a chiral ligand and oleic acid modified ferroferric oxide nano particles under the condition of emulsification-solvent volatilization. The chiral iron-based superparticle nano-material has a circular dichroism signal and a magnetic resonance signal at the same time, the used chiral ligand can have a strong cooperation effect with an iron element and can replace oleic acid molecules on the surface of ferroferric oxide, and then magnetic nano-particles are induced to generate chiral optical activity; the chiral ligand on the surface of the particle can change the magnetic relaxation ability of the chiral iron-based superparticle nano-material and enhance the imaging ability of the chiral iron-based superparticle nano-material ...

Hybridoma cell strain secreting famoxadone monoclonal antibody and application thereof

The invention discloses a hybridoma cell strain secreting a famoxadone monoclonal antibody and application of the hybridoma cell strain, and belongs to the field of immunodetection. The hybridoma cell strain secreting the famoxadone monoclonal antibody is preserved in the China General Microbiological Culture Collection Center (CGMCC) on February 8, 2023, and the preservation number is CGMCC No.45462. The famoxadone monoclonal antibody secreting the famoxadone monoclonal antibody has the advantages that the famoxadone monoclonal antibody secreting the famoxadone monoclonal The obtained famoxadone monoclonal antibody cell strain can be used for immunoassay detection, has good detection sensitivity and specificity to famoxadone, has an IC50 value of 1.31 ng/mL and a famoxadone analogue cross-over rate of less than 1%, can be used for preparing famoxadone immunodetection products, and has wide application prospects. And an efficient detection method and means are provided for residue detection ...

Fluoxetine hapten, monoclonal antibody, hybridoma cell strain and application

The invention discloses a fluoxetine hapten, a monoclonal antibody, a hybridoma cell strain and application, and belongs to the field of food safety immunodetection. According to the preparation method of the fluoxetine artificial antigen, the preparation process is simpler and more efficient, and the necessary artificial antigen is provided for research of people in the future; the hybridoma cell strain secreting the fluoxetine monoclonal antibody is provided and applied to fluoxetine immunoassay method research, the monoclonal antibody prepared from the hybridoma cell strain has good specificity and detection sensitivity to fluoxetine, IC50 of the monoclonal antibody to fluoxetine reaches 1 ng/mL, the monoclonal antibody can be used for establishing an immunological detection method of fluoxetine, and the monoclonal antibody can be applied to the immunoassay method research of fluoxetine. Or a colloidal gold immunochromatography test strip rapid detection method is established, and a ...

Optical device, lighting method, lighting device and sequencer

The invention provides an optical device, a lighting method, a lighting device and a sequencer, the optical device comprises a light source optical fiber, a collimating lens group, an afocal zoom lens group and a beam splitting lens group which are arranged in sequence, and the light source optical fiber is used for allowing a laser beam emitted by a laser light source to pass through and limiting the section of the laser beam into a preset shape; the collimating lens group is used for converging laser beams output by the light source optical fiber, the afocal zoom lens group is used for adjusting the size of the laser beams output by the collimating lens group, and the light splitting lens group is used for splitting the laser beams output by the afocal zoom lens group in a second direction; therefore, the laser beam emitted by the laser light source can be divided into the plurality of strip-shaped laser beams according to the wavelength range through the plurality of optical elements ...

Application of chiral nano-material in preparation of medicine for inducing neuronal cells to generate IGF-1 (Insulin-like Growth Factor-1)

The invention discloses an application of a chiral nano-material in preparing a medicine for inducing neuronal cells to generate IGF-1 (Insulin-like Growth Factor 1), and provides a method for inducing the generation of the insulin-like growth factor 1 by the chiral nano-material under the action of light. Chiral Ni (OH) 2 nano-particles can be specifically combined with neuronal cell membrane protein NGL-1; mechanical force is generated under the action of near-infrared light to induce the expression of IGF-1. The chiral nano material can regulate and control the IGF-1 in the cell and living body level, and the IGF-1 is crucial for maintaining the normal physiological needs of the body, especially for the healthy development of the brain and the regeneration of damaged neurons. Therefore, the invention has important significance in treatment of nervous system diseases such as spinal cord injury and the like.

Hybridoma cell strain capable of secreting monoclonal multi-residue antibody resisting phenobarbital drugs and application of hybridoma cell strain

The invention provides a hybridoma cell strain capable of secreting monoclonal multi-residue antibodies resisting phenobarbital drugs and application of the hybridoma cell strain, and belongs to the technical field of food safety immunodetection. A phenobarbital complete antigen and a Freund's reagent are mixed, emulsified and injected to immunize a BALB/c mouse, splenocytes and myeloma cells of the mouse with high titer and high inhibition are fused, the cells are screened through culture medium culture and indirect competitive enzyme-linked immunosorbent assay, multiple subcloning is carried out, and the phenobarbital complete antigen is obtained. The hybridoma cell strain secreting the monoclonal multi-residue antibody resisting the phenobarbital drugs is obtained. The phenobarbital drug monoclonal multi-residue antibody secreted by the cell strain has good affinity and high sensitivity to phenobarbital drugs, the 50% inhibitory concentration (IC50) of the phenobarbital drugs is 14.29 ...

Chiral iron particle as well as preparation method and application thereof

The invention relates to chiral iron particles and a preparation method and application thereof, and belongs to the technical field of inorganic chemistry. According to the preparation method of the chiral iron particle, a chiral ligand and an iron salt react in a solvent under the action of a reducing agent to obtain the chiral iron particle, the chiral ligand is amino acid; the amino acid is L-amino acid or D-amino acid. The chiral iron particle has excellent optical activity, has CD activity in a visible light region, and can be used for identification and detection of polarized light by using CD in the visible light region. The chiral iron particle has excellent magnetism and can be used in the technical fields of magnetic separation, biological imaging, adjuvants and the like. The chiral iron particle adjuvant can be attached to the surface of a cell membrane, enters dendritic cells, and can deliver antigens and maintain the stability of the antigens.

Preparation method of chiral metal oxide immunologic adjuvant and vaccine based on adjuvant

The invention relates to a preparation method of a chiral metal oxide immunologic adjuvant and a vaccine based on the adjuvant.The immunologic adjuvant is obtained through reaction of a surfactant, an alkaline solution, sugar ligands and metal salt, and preferably, the sugar ligands are L-type mannose and N-acetyl-D-glucosamine. The chiral nano vaccine can activate a Toll-like receptor pathway, inflammasome protein and interferon gene stimulating protein at the same time so as to activate T cells and B cells, has a strong cellular immune effect, can promote an organism to generate high-strength antibody titer, has a strong humoral immune effect, and can be used for preparing a novel anti-inflammatory drug. According to the present invention, the tumor immune escape can be inhibited by reducing PD-L1 and regulating negative costimulatory factors such as CTLA-4 and the like on the surface of the T cell, such that the related diseases can be well prevented and treated.

Synthesis method of glycosyl ligand analogue induced chiral cobalt-based ultra-small nanoparticles

According to the synthesis method, gluconic acid or sialic acid is taken as a chiral ligand, cobalt chloride hexahydrate is taken as a cobalt source, and in the presence of an alkaline solution or an alkaline reducing agent, stirring reaction is carried out to induce generation of the chiral cobalt-based ultra-small nanoparticles. The two cobalt-based ultra-small nano particles synthesized by the method have good chiral optical activity in a visible light region, and have certain guiding significance for preparing a glycosylated ligand modified chiral ultra-small nano material and researching mechanism analysis of formation of a glycosylated ligand induced chiral material.

Hybridoma cell strain secreting melatonin monoclonal antibody and application thereof

The invention relates to a hybridoma cell strain secreting a melatonin monoclonal antibody and application of the hybridoma cell strain, and belongs to the technical field of immunodetection. The melatonin complete antigen is prepared, the melatonin complete antigen and an equal amount of Freund's adjuvant are mixed and emulsified completely, BALB/c mice are immunized through back subcutaneous multipoint injection, the Freund's complete adjuvant is used for first immunization, the Freund's incomplete adjuvant is used for multiple booster immunization, and only the complete antigen is used for sprint immunization on the abdominal cavity of the mice at the last time. The melatonin monoclonal antibody hybridoma cell strain is obtained by selecting high-titer and low-IC50 mouse splenocytes to be fused with myeloma cells, screening through an indirect competitive enzyme-linked immunosorbent assay, and subcloning for three times. The monoclonal antibody secreted by the cell strain has better ...