05-05-2023 дата публикации
Номер: CN116064689A
Принадлежит:
The invention discloses a method for synthesizing 2, 2 ', 4, 5, 5'-pentachloro-4-biphenyl alcohol based on a pig liver microsome incubation system, which comprises the following steps: establishing a pig liver microsome incubation system, adding a substrate 2, 2 ', 4, 5, 5'-pentachlorobiphenyl, and transforming 2, 2 ', 4, 5, 5'-pentachlorobiphenyl into 2, 2 ', 4, 5, 5'-pentachloro-4-biphenyl alcohol by metabolism of pig liver microsome; after the incubation is terminated, isopropanol, dichloromethane and normal hexane are added into the incubation liquid, after oscillation and centrifugation, an organic phase is transferred, and the organic phase is volatilized under the condition of high temperature and reduced pressure; according to the method, 2, 2 ', 4, 5, 5'-pentachloro-4-biphenyl alcohol is directionally converted into 2, 2 ', 4, 5, 5'-pentachloro-4-biphenyl alcohol, lipid impurities are removed through concentrated sulfuric acid sulfonation reaction, residual 2, 2 ', 4, 5, 5'-pentachlorobiphenyl ...
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