18-04-2023 дата публикации
Номер: CN115975926A
Принадлежит:
The invention relates to a hematopoietic stem cell serum-free culture medium and application thereof.The culture medium is prepared by mixing a basic culture medium and additive components, and the additive components comprise stem cell factors (SCF), recombinant human thrombopoietin (TPO), recombinant human Flt3 ligands (Flt3-L), rhIL-6, rhIL-3, CITED2, HSF1A, OAC1, recombinant human insulin, recombinant human apotransferrin, recombinant human serum albumin and ethanolamine. And sodium selenite. The basic culture medium is IMDM. The culture medium is clear in chemical component, free of serum, free of human-derived and animal-derived components, simple in formula and excellent in performance, the amplification capacity of the hematopoietic stem cells can be remarkably improved, the hematopoietic stem cells can be amplified by 500 times or above after being cultured for 13 days, the cell viability can reach 95% or above during harvesting, and the positive proportion of CD34 + cells is remarkably ...
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