08-12-2017 дата публикации
Номер: KR1020170135351A
Принадлежит:
The present invention relates to a multiplex-PCR method for selecting an enterococcus strain and, more specifically, to a multiplex-PCR method using a template as a colony by targeting four species of enterococcus (E. faecalis, E. faecium, E. hirae, E. durans). The present invention can identify bacteria of four species of enterococcus (E. faecalis, E. faecium, E. hirae, E. durans) in a simple and economical method by, with a newly designed computer programming analysis method, searching a gene specifically in each of enterococcus species, producing a primer, and performing a PCR, and thus checking the identification of existing bacteria, which could be known only through sequencing, through a PCR method. Moreover, the present invention can save time and is economical when developing feed additives and probiotics spending most time in isolating and identifying bacteria since analysis and appliance on other genus such as lactobacillus, bacillus as well as enterococcus are possible based ...
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