Resonance scattering spectrometry for measuring ammonia nitrogen in water rapidly

The present invention discloses a resonance scattering spectrometry for measuring ammonia nitrogen in water rapidly, the measurement steps is that 1. a standard system of ammonium muriate is prepared, and a scattering intensity I is mensurated at a point of 447m; 2. a blank system without ammonium muriate is prepared, and a scattering intensity I0 is mensurated; 3. calculation of Delta I447m = I-I0; 4. a working curve is made according to the concentration relation of the Delta I447m to the ammonium muriate; 5. a detection system is prepared by adding a measuring object containing ammonium muriate with ignorance concentration, and Delta I447M of the measuring system is obtained; 6. according to the working curve, the ammonium muriate concentration of the measuring object is calculated. Comparing with the existing method, the instrument of the method of the invention is simple, operation is easy, reagent is obtained easily, sensitivity is high, measurement range is wide, and cost is low.

CdTe quantum point fluorimetric determination method of chlorine dioxide in water

The present invention discloses a CdTe quantum dot fluorimetric determination method for detecting the chlorine dioxide in the water. In the 5mL color comparison tubes, the CdTe quantum dot systems with experiment grain diameters respectively for 4.0nm, 4.6nm, 5.2nm, 6.5nm and 8.6nm are respectively added with the following solutions with different volume: a CdTe solution with concentration for 6.30*10<-5>mol/L; NH4Cl-NH3 buffering solution which has a pH value of 8.0-9.1 for dosage 0.1-1.0Ml, and the chlorine dioxide with concentration 10.62mu g/mL respectively for 0.040-1.50mL, 0.10-1.0mL, 0.080-1.5mL, 0.015-0.80mL and 0.050-1.0mL. The secondary distilled water is added in to 2.0mL and is shaken up. The fluorescence intensity value F of each system when the wavelength is respectively 632.05nm, 665.94nm, 665.94nm, 709.00nm and 748.05nm. A fluorescence quenching intensity value deltaF is calculated according to the equation: deltaF=F0-F. Additionally water with right amount is added into ...

Method for measuring sulphite in food using chlorauric acid resonance scattering spectrometry

The present invention discloses a method for measuring the sulfite in the food with a chlorauric acid resonance scattering spectrometric determination. A 5mL color comparison tube is sequentially added with a 223.2 mu g/mL (calculated with Au(III)) HAuCl4 for 0.10-1.50mL, 1.0% aminosulfonic solution for 0.05-0.5mL and 116.0 mu g/mL sodium sulfite for 0.05-1.50mL, shaking up, adding the secondary distilled water to volume, swaying to uniform, and placing for 5-180min. On a fluorospectrophotometer, a synchronous scanning in which the inspiring wavelength is equal to the transmission wavelength is adopted, and the resonance scattering spectrometric of the system is obtained. The intensity I583nm of scattering light and reagent vacancy I0 are measured at 583nm. The value of delta I583nm is calculated with the equation: delta I583nm=I583nm-I0. Additionally the white sugar with right amount is added and is dissolved in water. The value of intensity delta I583nm is measured according to the method ...

Method for measuring sulphite in food using chlorauric acid resonance scattering spectrometry

The present invention discloses a method for measuring the sulfite in the food with a chlorauric acid resonance scattering spectrometric determination. A 5mL color comparison tube is sequentially added with a 223.2 mu g/mL (calculated with Au(III)) HAuCl4 for 0.10-1.50mL, 1.0% aminosulfonic solution for 0.05-0.5mL and 116.0 mu g/mL sodium sulfite for 0.05-1.50mL, shaking up, adding the secondary distilled water to volume, swaying to uniform, and placing for 5-180min. On a fluorospectrophotometer, a synchronous scanning in which the inspiring wavelength is equal to the transmission wavelength is adopted, and the resonance scattering spectrometric of the system is obtained. The intensity I583nm of scattering light and reagent vacancy I0 are measured at 583nm. The value of delta I583nm is calculated with the equation: delta I583nm=I583nm-I0. Additionally the white sugar with right amount is added and is dissolved in water. The value of intensity delta I583nm is measured according to the method ...

Method for measuring vitamin C by gold nano particulate spectrophotometry

The invention discloses a method for determining vitamin C by a golden nanometer particle spectrophotometry way. In a 5mL colorimetric cylinder, sequentially 0.1-2.0 mL HAuCl4 in concentration 95.64 Mu g/mL, 0.02-0.50 mL Sodium Citrate in concentration 1%, and 0.001-2.0mL vitamin C solvent in concentration 0.38mg/mL are added, mixed evenly, then some redistilled water is added to the graduation, mixed fully again; the absorption value is measured on a spectrophometer; meanwhile a blank test is conducted. The determining method is simple and rapid, the instrument used is cheap, and the reagent is easily available.

Method for purifying nano silver fine particle by high-speed centrifugation

The invention discloses a method for purifying silver nanometer particles high-speedily and centrifugally, which is characterized in that silver gel which is prepared is arranged in a centrifuge tube and purified high-speedily and centrifugally, and thereby the silver nanometer particles with high pureness and good stability can be obtained. The invention has the advantages that the method for purifying is simple and reliable, the pureness of purity is high, and the stability is good.

Method for measuring vitamin C by gold nano particulate spectrophotometry

Method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold

The invention discloses a method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold, which comprises the following steps: Hg2+ aptamer ssDNA and nanogold are combined to form nanogold AussDNA modified by DNA, stable Hg2+aptamer-Hg2+compound is generated by T-T mismatching when Hg2+ and AussDNA coexist, nanogold is gathered under the action of high-concentration NaCl, redundant AussDNA in filtrate after membrane filtration is taken as a nano catalytic seed crystal to catalyze the reaction of Cu2+ and furfural so as to reduce Cu2+ into Cu+, Cu+ reacts with OH- in solution to generate CuOH, and finally generate Cu2O particles. From the above preparation method, a determined system and a reagent blank system of mercury concentration can be known, resonance scattering strength of the two systems are measured at the 605nm part, drawn into a standard curve, and then prepared into a sample detection system, a deltaI sample is solved, the mercury ...

Method for measuring trace Hg2+ by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum

The invention discloses a method for detecting trace Hg2+ by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum. T-T mismatch in an ssDNA sequence can be stabilized by using Hg2+ so that the ssDNA cannot stabilize AuRe composite nano particles and the AuRe composite nano particles are gathered under the high-concentration electrolyte condition, and the phenomenon and a nano gold rhenium catalysis reaction are organically combined; and the tellurium particles generated by the catalytic reaction have resonance scattering; and the Hg2+ and the resonance scattering strength are in linear relation. A test system with known mercury concentration and a reagent vacant system are first prepared during the measurement; the resonance scattering strength with wavelengths of 734nm of the two systems are measured; a working curve is drawn; then, a sample detection system is prepared; a delta I sample is obtained; and the content of the mercury in the sample ...

Nano argentum spectrophotometry for detecting hydroxy free radical

Kit for detecting immuno-nanogold synchronous scattering spectrum of human serum ceruloplosmin and use method thereof

The invention discloses a kit for detecting immuno-nanogold synchronous scattering spectrum of human serum ceruloplosmin and a use method thereof. The kit comprises three reagents, wherein the reagent 1 contains 0.001-0.20mol/L of citric acid, 0.1-0.3mol/L of disodium hydrogen phosphate solution and 100-400g/L of PEG 6000 sensitizer, the reagent 2 contains 50-200g/L of PEG 20000 stabilizer and 30-60mu g/ml of nanogold-labeled goat anti-human ceruloplosmin (AuGCP) antibody, and the reagent 3 is a calibrator, namely a ceruloplosmin (CP) calibrator solution. The specific use method comprises the following steps: firstly drawing a synchronous scattering intensity-concentration standard curve, and then calculating the CP content according to the synchronous scattering intensity of a sample to be detected and the standard curve. The kit has simple and fast and operation, high sensitivity and strong specificity.

Method for measuring trace Hg2+ by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum

The invention discloses a method for detecting trace Hg<2+> by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum. T-T mismatch in an ssDNA sequence can be stabilized by using Hg<2+> so that the ssDNA cannot stabilize gold-rhenium composite nano particles and the gold-rhenium composite nano particles are gathered under the high-concentration electrolyte condition, and the phenomenon and a nano gold rhenium catalysis reaction are organically combined; and the tellurium particles generated by the catalytic reaction have resonance scattering; and the Hg<2+> and the resonance scattering strength are in linear relation. A test system with known mercury concentration and a reagent vacant system are first prepared during the measurement; the resonance scattering strength with wavelengths of 734nm of the two systems are measured; a working curve is drawn; then, a sample detection system is prepared; a delta I sample is obtained; and the content of ...

Enzyme catalysis oxidation nanometer silver-graphite oven atomic absorption spectrometry for mensuration of horse radish peroxidase

The present invention discloses an enzyme catalysis oxidation nanometer silver-graphite oven atomic absorption spectrometry for mensuration of horse radish peroxidase, the mensuration steps is that 1.a standard system of HRP is prepared firstly, and the absorbency Ab of the standard system is mensurated through a graphite oven automic absorption spectrophotometer; 2. a blank control system without HRP is prepared, and the absorbency A0 of the blank control system is mensurated; 3. calculation of Delta A = Ab-A0; 4. a working curve is made according to the concentration relation of the Delta Aand the HRP; 5. a detection system is prepared, and is added with a measuring object containing HRP with ignorance concentration, then the absorbency Delta A is mensurated; 6. according to the working curve, the concentration of the HRP of the measuring object is obtained. The spectrometry of the invention has a better selectivity and a high sensitivity; the reaction condition is reached easily,and ...

Resonance scattering spectrometry for rapidly measuring ammonia nitrogen in water

The present invention discloses a resonance scattering spectrometry for measuring ammonia nitrogen in water rapidly, the measurement steps is that 1. a standard system of ammonium muriate is prepared,and a scattering intensity I is mensurated at a point of 447m; 2. a blank system without ammonium muriate is prepared, and a scattering intensity I0 is mensurated; 3. calculation of Delta I447m = I-I0; 4. a working curve is made according to the concentration relation of the Delta I447m to the ammonium muriate; 5. a detection system is prepared by adding a measuring object containing ammonium muriate with ignorance concentration, and Delta I447M of the measuring system is obtained; 6. according to the working curve, the ammonium muriate concentration of the measuring object is calculated. Comparing with the existing method, the instrument of the method of the invention is simple, operation is easy, reagent is obtained easily, sensitivity is high, measurement range is wide, and cost is low.

Method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold

The invention discloses a method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold, which comprises the following steps: Hg2+ aptamer ssDNA and nanogold are combined to form nanogold AussDNA modified by DNA, stable Hg2+aptamer-Hg2+compound is generated by T-T mismatching when Hg2+ and AussDNA coexist, nanogold is gathered under the action of high-concentration NaCl, redundant AussDNA in filtrate after membrane filtration is taken as a nano catalytic seed crystal to catalyze the reaction of Cu2+ and furfural so as to reduce Cu2+ into Cu+, Cu+ reacts with OH- in solution to generate CuOH, and finally generate Cu2O particles. From the above preparation method, a determined system and a reagent blank system of mercury concentration can be known, resonance scattering strength of the two systems are measured at the 605nm part, drawn into a standard curve, and then prepared into a sample detection system, a deltaI sample is solved, the mercury ...

Enzyme catalysis oxidation nanometer silver-graphite oven atomic absorption spectrometry for mensuration of horse radish peroxidase

The present invention discloses an enzyme catalysis oxidation nanometer silver-graphite oven atomic absorption spectrometry for mensuration of horse radish peroxidase, the mensuration steps is that 1. a standard system of HRP is prepared firstly, and the absorbency Ab of the standard system is mensurated through a graphite oven automic absorption spectrophotometer; 2. a blank control system without HRP is prepared, and the absorbency A0 of the blank control system is mensurated; 3. calculation of Delta A = Ab-A0; 4. a working curve is made according to the concentration relation of the Delta A and the HRP; 5. a detection system is prepared, and is added with a measuring object containing HRP with ignorance concentration, then the absorbency Delta A is mensurated; 6. according to the working curve, the concentration of the HRP of the measuring object is obtained. The spectrometry of the invention has a better selectivity and a high sensitivity; the reaction condition is reached easily, and ...

Method for purifying nano silver fine particle by high-speed centrifugation

The invention discloses a method for purifying silver nanometer particles high-speedily and centrifugally, which is characterized in that silver gel which is prepared is arranged in a centrifuge tube and purified high-speedily and centrifugally, and thereby the silver nanometer particles with high pureness and good stability can be obtained. The invention has the advantages that the method for purifying is simple and reliable, the pureness of purity is high, and the stability is good.

Nano argentum spectrophotometry for detecting hydroxy free radical

The invention relates to a nanometer silver spectrophotometer which is provided with the nanometer silver as hydroxyl radical scavenger and is a method that determines the absorbency of tested systems with the spectrophotometer. The hydroxyl radical is tested as follows, (1) rationed nanometer silver is taken to be evenly mixed with tested liquid of known consistency and the tested liquid contains or can produce the hydroxyl radical, and the tested systems are formed after constant volume with water; (2) the tested systems is transferred to a cuvette and absorbance A is determined with the spectrophotometer at 460nm; (3) the nanometer silver as the blank contrast is taken to determine the absorbance A₀; (4) Delta A= A₀-A is determined; (5) a specification curve is made out according to the correspondence of the values of the hydroxyl radical consistency C and delta A of the tested systems of different consistency; (6) the value of delta A of the tested system of unknown ...