Resonance scattering spectrometry for measuring ammonia nitrogen in water rapidly

The present invention discloses a resonance scattering spectrometry for measuring ammonia nitrogen in water rapidly, the measurement steps is that 1. a standard system of ammonium muriate is prepared, and a scattering intensity I is mensurated at a point of 447m; 2. a blank system without ammonium muriate is prepared, and a scattering intensity I0 is mensurated; 3. calculation of Delta I447m = I-I0; 4. a working curve is made according to the concentration relation of the Delta I447m to the ammonium muriate; 5. a detection system is prepared by adding a measuring object containing ammonium muriate with ignorance concentration, and Delta I447M of the measuring system is obtained; 6. according to the working curve, the ammonium muriate concentration of the measuring object is calculated. Comparing with the existing method, the instrument of the method of the invention is simple, operation is easy, reagent is obtained easily, sensitivity is high, measurement range is wide, and cost is low.

Organic W-Mo friction improver and its preparation process

An organic W or Mo friction improving agent is a resultant of the reaction of rapeseed oil (60-90 pts. wt.), diethanolamine (18-25 pts. wt.), Mo-containing compound (7-9 pts. wt.) and tungsten salt (1-1.5 pts. wt.).Said Mo-containing compound includes molybdenum salt and its oxide. Its advantages are high performance to reduce friction and resist abrasion and corrosion, and no corrosion, odour and pollution.

Organic wolfram-molybdenum friction improver and its producing method

Resonance Rayleigh scattering energy transfer spectroscopy for simply and rapidly measuring formaldehyde

The invention discloses a resonance Rayleigh scattering energy transfer spectroscopy for simply and rapidly measuring formaldehyde. The resonance Rayleigh scattering energy transfer spectroscopy comprises the following steps: (1) preparing a formaldehyde standard solution system; (2) preparing a blank control solution system; (3) respectively measuring resonance Rayleigh scattering energy transfer peak intensity values I(standard) and I(blank) of the formaldehyde standard solution system and the blank control solution system, and calculating delta I which is equal to difference of I(blank) to I(standard); (4) drawing a working curving of a relation between concentration of formaldehyde and delta I; (5) measuring a sample of a measured object, and calculating delta I(sample) which is equal to difference of I(blank) to I(sample); and (6) calculating the concentration of formaldehyde in the measured object from the working curve in the step (4) according to the measured delta I(sample) of ...

Method for quickly preparing stable nano-gold sol

The invention discloses a method for quickly preparing stable nano-gold sol. The method is characterized in that a certain amount of water is mixed with chloroauric acid, trisodium citrate and hydrogen peroxide in different concentrations, the color of reaction solution is colorless at first and is then gradually turned into red, and the nano-gold sol which has good stability and high SERS (Surface Enhanced Raman Scattering) activity can be obtained after 15 min. The method for quickly preparing the stable nano-gold sol, disclosed by the invention, has the advantages that compared with the prior art, raw materials are easily obtained and do not need to be heated, liquid-phase nano-gold which has the high SERS activity can be obtained just by one-step reaction, the preparing method is simpler and quicker, the stability is high, and the dispersibility is good.

Method for measuring activity of chymotrypsin or trypsin or elastase or pepsin

The invention discloses a method for testing the activity of alfapsin, parenzyme, elastase or pepsase, which comprises using pepsase as substrate, adding the enzymes to catalyze, using trichloroacetic acid to stop the enzyme catalysis reaction and be reacted with left substrates to form association particles to improve the resonance scattering strength, thereby building a method for testing the activity of alfapsin, parenzyme, elastase or pepsase, while the enzyme activity density can be calculated from resonance scattering correct value curvature. The invention has the advantages that compared with prior art, the invention combines enzyme catalysis reaction with resonance scattering spectrum, with high analysis sensitivity, better selectivity, low check limit and simple operation, while utilized agents are non-toxic and safe.

Nano gold catalysis resonance scattering spectrometry for measuring grape-sugar

The invention discloses a nanometer gold catalysis resonance scattering spectrum method for testing amylaceum, which is based on the catalysis of nanometer gold on the copper bromide particles generated by Cu(II)-amylaceum-KBr, and the linear relationship between amylaceum in some density and delta I610nm. The invention has the advantages that compared with prior art, the invention combines catalysis reaction with resonance scattering spectrum technique, with high analysis sensitivity, low check limit, ug/mL level, better selectivity, simple operation, simple device, easily accessible agents and low cost, which only needs fluorescence spectrometer.

Method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold

The invention discloses a method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold, which comprises the following steps: Hg2+ aptamer ssDNA and nanogold are combined to form nanogold AussDNA modified by DNA, stable Hg2+aptamer-Hg2+compound is generated by T-T mismatching when Hg2+ and AussDNA coexist, nanogold is gathered under the action of high-concentration NaCl, redundant AussDNA in filtrate after membrane filtration is taken as a nano catalytic seed crystal to catalyze the reaction of Cu2+ and furfural so as to reduce Cu2+ into Cu+, Cu+ reacts with OH- in solution to generate CuOH, and finally generate Cu2O particles. From the above preparation method, a determined system and a reagent blank system of mercury concentration can be known, resonance scattering strength of the two systems are measured at the 605nm part, drawn into a standard curve, and then prepared into a sample detection system, a deltaI sample is solved, the mercury ...

Method for measuring trace Hg2+ by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum

The invention discloses a method for detecting trace Hg2+ by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum. T-T mismatch in an ssDNA sequence can be stabilized by using Hg2+ so that the ssDNA cannot stabilize AuRe composite nano particles and the AuRe composite nano particles are gathered under the high-concentration electrolyte condition, and the phenomenon and a nano gold rhenium catalysis reaction are organically combined; and the tellurium particles generated by the catalytic reaction have resonance scattering; and the Hg2+ and the resonance scattering strength are in linear relation. A test system with known mercury concentration and a reagent vacant system are first prepared during the measurement; the resonance scattering strength with wavelengths of 734nm of the two systems are measured; a working curve is drawn; then, a sample detection system is prepared; a delta I sample is obtained; and the content of the mercury in the sample ...

Resonance Rayleigh scattering spectrometry for quickly determining sodium hexametaphosphate

The invention discloses a resonance Rayleigh scattering spectrometry for simply and quickly determining sodium hexametaphosphate. The resonance Rayleigh scattering spectrometry comprises the steps: (1) preparing a sodium hexametaphosphate standard solution system; (2) preparing a blank contrast solution system; (3) respectively determining resonance Rayleigh scattering peak strength values I standard and I blank of the sodium hexametaphosphate standard solution system and the blank contrast solution system, and performing the formula: delta I = I standard - I blank; (4) drafting a working curve of the concentration relation of the sodium hexametaphosphate by using the delta I; (5) determining a sample of an object to be determined, and performing the formula: delta I sample = I sample - I blank; (6) checking the working curve draft in step (4) according to the obtained delta I sample, and calculating the content of the sodium hexametaphosphate in the object to be determined. The resonance ...

Method for measuring trace Hg2+ by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum

The invention discloses a method for detecting trace Hg<2+> by using aptamer modified nano gold rhenium catalysis-tellurium particle resonance scattering spectrum. T-T mismatch in an ssDNA sequence can be stabilized by using Hg<2+> so that the ssDNA cannot stabilize gold-rhenium composite nano particles and the gold-rhenium composite nano particles are gathered under the high-concentration electrolyte condition, and the phenomenon and a nano gold rhenium catalysis reaction are organically combined; and the tellurium particles generated by the catalytic reaction have resonance scattering; and the Hg<2+> and the resonance scattering strength are in linear relation. A test system with known mercury concentration and a reagent vacant system are first prepared during the measurement; the resonance scattering strength with wavelengths of 734nm of the two systems are measured; a working curve is drawn; then, a sample detection system is prepared; a delta I sample is obtained; and the content of ...

Nano gold catalysis resonance scattering spectrometry for measuring grape-sugar

The invention discloses a nanometer gold catalysis resonance scattering spectrum method for testing amylaceum, which is based on the catalysis of nanometer gold on the copper bromide particles generated by Cu(II)-amylaceum-KBr, and the linear relationship between amylaceum in some density and delta I610nm. The invention has the advantages that compared with prior art, the invention combines catalysis reaction with resonance scattering spectrum technique, with high analysis sensitivity, low check limit, ug/mL level, better selectivity, simple operation, simple device, easily accessible agents and low cost, which only needs fluorescence spectrometer.

Resonance scattering spectrometry for rapidly measuring ammonia nitrogen in water

The present invention discloses a resonance scattering spectrometry for measuring ammonia nitrogen in water rapidly, the measurement steps is that 1. a standard system of ammonium muriate is prepared,and a scattering intensity I is mensurated at a point of 447m; 2. a blank system without ammonium muriate is prepared, and a scattering intensity I0 is mensurated; 3. calculation of Delta I447m = I-I0; 4. a working curve is made according to the concentration relation of the Delta I447m to the ammonium muriate; 5. a detection system is prepared by adding a measuring object containing ammonium muriate with ignorance concentration, and Delta I447M of the measuring system is obtained; 6. according to the working curve, the ammonium muriate concentration of the measuring object is calculated. Comparing with the existing method, the instrument of the method of the invention is simple, operation is easy, reagent is obtained easily, sensitivity is high, measurement range is wide, and cost is low.

Method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold

The invention discloses a method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold, which comprises the following steps: Hg2+ aptamer ssDNA and nanogold are combined to form nanogold AussDNA modified by DNA, stable Hg2+aptamer-Hg2+compound is generated by T-T mismatching when Hg2+ and AussDNA coexist, nanogold is gathered under the action of high-concentration NaCl, redundant AussDNA in filtrate after membrane filtration is taken as a nano catalytic seed crystal to catalyze the reaction of Cu2+ and furfural so as to reduce Cu2+ into Cu+, Cu+ reacts with OH- in solution to generate CuOH, and finally generate Cu2O particles. From the above preparation method, a determined system and a reagent blank system of mercury concentration can be known, resonance scattering strength of the two systems are measured at the 605nm part, drawn into a standard curve, and then prepared into a sample detection system, a deltaI sample is solved, the mercury ...

Benzoyl peroxide measuring method by blue nanometer silver sol spectrophotometry

The invention discloses a benzoyl peroxide measuring method by blue nanometer silver sol spectrophotometry. The measuring method includes steps of: (1) preparing a standard benzoyl peroxide solution system; (2) preparing a blank control solution system; (3) respectively taking a proper amount of the standard benzoyl peroxide solution system in the step (1) and the blank control solution system in the step (2), putting on cuvettes, scanning on a spectrophotometer to obtain spectrograms of the systems, measuring the absorbance value A of the BPO standard solution system at the wavelength of 558 nm, measuring the absorbance value A0 of the blank control solution system, and calculating [delta]A according to a formula of [delta]A=A0-A; (4) drawing a working curve of [delta]A against the concentration of the BPO; (5) measuring a sample of an analyte; and (6) checking the working curve in the step (4) according to the [delta]A of the sample, and calculating the concentration of the BPO ...

Resonance Rayleigh scattering energy transfer spectroscopy method for determining fluorinion simply and rapidly

The invention discloses a resonance Rayleigh scattering energy transfer spectroscopy method for determining a fluorinion simply and rapidly. The method comprises the following steps: (1) preparing a fluorinion standard solution system; (2) preparing a blank reference solution system; (3) determining the resonance Rayleigh scattering energy transfer intensity values I(standard) and I(blank) of the fluorinion standard solution system and the blank reference solution system respectively, and calculating delta I=I (blank)-I (standard); (4) drawing a working curve for the concentration relationship of the fluorinion by delta I; (5) determining a detected object sample, and calculating delta I (sample)=I(blank)-I (sample); and (6) checking the working curve of the step (4) according to the determined delta I of the sample, and calculating the concentration of the fluorinion in a detected object. According to the resonance Rayleigh scattering energy transfer spectroscopy method disclosed by the ...