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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 5. Отображено 5.
25-07-2023 дата публикации

Construction and application of avian leukosis virus recombinant p27 protein genetic engineering cell line

Номер: CN116479045A
Автор: RONG FANG, LI ZUOSHENG, CHEN RUI'AI, CHEN GUI'E, SUN RONGHANG, FENG XIANING, LIU YUFU, HAO WENQIAN
Принадлежит:

The invention belongs to the technical field of genetic engineering and biological detection, and discloses construction and application of an avian leukosis virus recombinant p27 protein genetic engineering cell line. The construction method comprises the following steps: adding a Kozac sequence before an initiation codon ATG according to sequence information of an avian leukosis virus p27 protein gene, connecting a mouse IgGkappa chain signal peptide sequence to the N end of the sequence, and designing a histidine tag and a Flag tag at the C end of the sequence to obtain a modified p27 sequence; then, the modified p27 sequence is cloned to a pCMV-EGFP carrier, and a recombinant expression plasmid pCMV-EGFP-p27 is constructed and obtained; the recombinant expression plasmid is transformed to transfect CHO-K1 cells, then G418 pressurized screening is carried out to obtain monoclonal cells, and amplification culture is carried out to obtain the avian leukosis virus recombinant p27 protein ...

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Номер записи: 1
08-08-2023 дата публикации

Special nursing device for epilepsy patient

Номер: CN116548922A
Автор: YUAN PING, HE JIN, CHEN GUI'E, WANG ZUOHUA, XUN JIANMEI, YANG YUNZHEN, YANG XUEJIAO
Принадлежит:

The invention discloses a special nursing device for an epilepsy patient, and relates to the field of nursing devices.The special nursing device specifically comprises a nursing cap, a brain wave monitor and a monitoring bracelet, preformed holes for placing ears of a child patient are formed in the two sides of the nursing cap, and a cavity for placing a reinforcing plate is formed in the nursing cap; grooves for placing a plurality of electrode patches are formed in the inner surface of the nursing cap at equal intervals, a first hook-and-loop fastener hook face is fixed to the outer portion of the nursing cap, and the first hook-and-loop fastener hook face is fixed to the external cover by being attached to the first hook-and-loop fastener loop face. After the single-chip microcomputer detects that the brain wave monitor detects a brain wave abnormal signal, the single-chip microcomputer starts a switch of the audible and visual alarm to give an audible and visual alarm, then the single-chip ...

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Номер записи: 2
07-07-2023 дата публикации

PVDF photocatalytic self-cleaning film containing Z-type 2D/3D heterojunction as well as preparation method and application of PVDF photocatalytic self-cleaning film

Номер: CN116392973A
Автор: CHEN GUI'E, DENG SHUJUN, ZHAO SHENGNAN, TANG RUNHUA, HAN XIAOYANG, MA WENXIAO, ZHANG HAORAN, XU ZHENLIANG
Принадлежит:

The invention belongs to the technical field of membrane separation, and discloses a PVDF photocatalytic self-cleaning membrane containing a Z-type 2D/3D heterojunction and a preparation method and application thereof. The preparation method comprises the following steps: preparing a Z-type 2D/3Dg-C3N4/alpha-Fe2O3 heterojunction photocatalyst through a hydrothermal reaction, then mixing the Z-type 2D/3Dg-C3N4/alpha-Fe2O3 heterojunction photocatalyst with polyvinylidene fluoride to prepare a membrane casting solution, and preparing the polyvinylidene fluoride photocatalytic self-cleaning membrane through a non-solvent induced phase separation method. According to the preparation method, the heterojunction photocatalyst is added into the polyvinylidene fluoride membrane casting solution in the form of an additive, and the PVDF membrane is modified by introducing the heterojunction photocatalyst and an NIPS method, so that the membrane is endowed with photocatalytic self-cleaning performance ...

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Номер записи: 3
29-09-2010 дата публикации

N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid compound and preparation method thereof

Номер: CN0101845003A
Автор: CHEN GUI'E, XIA XIAOZHONG, XU ZHENLIANG, CHEN GUI E, XIAOZHONG XIA, ZHENLIANG XU
Принадлежит:

The invention relates to an N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid compound and a preparation method thereof. The N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid compound is prepared by the following steps of putting tris (hydroxymethyl) aminomethane and 1,3-propanesultone with the same molar ratio into a 500mL four-hole flask with a stirring paddle, a thermometer and a condensation pipe, adding 250-300mL of C1-C8 alcohol as a solvent, stirring and dissolving the mixture with a proper speed, heating a reaction system to boiling, and treating the alcohol by condensing reflux; reacting for 1-8h; putting a reaction product into a refrigerator, treating the product by cooling to 0 DEG C and filtering, wherein a filter cake is an obtained crude product; obtaining a white powder final product by washing the crude product by absolute ethyl alcohol for 2-3 times, and obtaining the N-tris (hydroxymethyl) methyl-3-aminopropanesulfonic acid compound by putting the final ...

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Номер записи: 4
18-07-2023 дата публикации

Monoclonal antibody for resisting African swine fever virus p72 protein as well as preparation method and application thereof

Номер: CN116444654A
Автор: CHEN GUI'E, FENG XIANING, CHEN RUI'AI, RONG FANG, SUN RONGHANG, LI ZUOSHENG, LIU YUFU
Принадлежит:

The invention belongs to the technical field of bioengineering, and discloses an anti-African swine fever virus p72 protein monoclonal antibody as well as a preparation method and application thereof. The preparation method comprises the following steps: expressing and purifying a recombinant plasmid pET28b-p72 to obtain a p72 protein immune mouse, carrying out intraperitoneal injection of p72 protein on the mouse with the highest serum antibody titer to carry out booster immunization, fusing splenocytes of the mouse subjected to booster immunization with sp2/0 cells, screening out positive hybridoma cells capable of secreting anti-p72 antibodies, and purifying the positive hybridoma cells to obtain the anti-p72 antibodies. And subcloning the positive hybridoma cell strain to obtain the monoclonal antibody cell strain for resisting the African swine fever virus p72 protein. The ASFV p72 protein is expressed by using a prokaryotic expression system, the cell strain capable of stably secreting ...

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Номер записи: 5