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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 28. Отображено 28.
08-03-2017 дата публикации

Protection device of hand -held type water quality measurement appearance

Номер: CN0206002523U
Автор: CHEN RUI'AI, MA GUANGMING, OUYANG ZHENGLIANG, GAO REN, JI HUASONG
Принадлежит: ORIENTAL DALIN OCEAN BIOLOGICAL CO LTD, ORIENTAL DALIN OCEAN BIOLOGICAL CO., LTD.

The utility model discloses a protection device of hand -held type water quality measurement appearance, characterized by, this protection device is including probe protection device and line pole, probe protection device is the beverage bottle type structure of fretwork, and the wall energy is closely inlayed with the probe afterbody of hand -held type water quality measurement appearance in its bottleneck, probe protection device bottom is with lid still, the line pole is the tubular structure of both ends intercommunication, line pole one end through can dismantle the mode with probe protection device's upper end is connected, and line pole and this probe protection device intercommunication. The device can effective guarantee pop one's head in, protect the protection device of connecting wire and display safety, and the device simple structure is firm, and the equipment is convenient.

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Номер записи: 1
26-05-2023 дата публикации

Primer pair for detecting infectious bronchitis virus as well as application and detection method thereof

Номер: CN116162737A
Автор: XIONG TING, CHEN RUI'AI, YU CHUANZHAO, LIANG SHIJIN, HE XIANMING, ZHUANG TINGTING
Принадлежит:

The invention discloses a primer pair and a fluorescent probe for detecting an infectious bronchitis virus as well as application and a detection method of the primer pair and the fluorescent probe, and relates to the technical field of biological virus detection. Wherein the nucleotide sequence of a forward primer in the primer pair is as shown in SEQ ID No.1, the nucleotide sequence of a reverse primer is as shown in SEQ ID No.2, and the nucleotide sequence of the fluorescent probe is as shown in SEQ ID No.3; the 5'end of the fluorescent probe is modified by FAM, and the 3 'end of the fluorescent probe is modified by TAMRA. By implementing the kit, IBV of various genotypes can be accurately detected, and a good basis is provided for early diagnosis of IBV.

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Номер записи: 2
02-05-2023 дата публикации

Reconstruction method of SFV recombinant replicon vector expression platform

Номер: CN116042718A
Автор: CHEN RUI'AI, HUANG MEI, FANG NIRAN, XIE WENTING, XU TING, LIU DINGXIANG
Принадлежит:

The invention belongs to the technical field of bioengineering, and discloses a reconstruction method of an SFV recombinant replicon vector expression platform. The reconstruction method comprises the following steps: respectively replacing an SP6 promoter in a pSFVCs-EGFP plasmid and an SP6 promoter in a pSFV-Helper1 auxiliary plasmid with a CMV (cytomegalovirus) promoter, so as to obtain a plasmid pSFV-EGFP-CMV and a plasmid pSFV-Helper1-CMV. The mode gene EGFP in the pSFV-EGFP-CMV is replaced by any antigen gene, so that a corresponding replicon plasmid which can express specific antigen protein and has self-replicating capability can be obtained. The pSFV-EGFP-CMV and the pSFV-Helper1-CMV are subjected to co-transfection, so that a replication particle which only has first-generation infectivity and is recombined can be obtained. The system can be safely applied to development of first-generation live virus vector vaccines and preparation of target proteins.

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Номер записи: 3
25-04-2023 дата публикации

Construction of CHO cell line capable of stably expressing porcine epidemic diarrhea virus S1 protein

Номер: CN116004546A
Автор: CHEN RUI'AI, LI ZUOSHENG, LIU YUFU, RONG FANG, HAO WENQIAN
Принадлежит:

The invention belongs to the technical field of biomedical engineering, and discloses construction of a CHO cell line capable of stably expressing porcine epidemic diarrhea virus S1 protein. The construction method comprises the following steps: adding a Kozac sequence in front of an initiation codon ATG of an S1 gene sequence of PEDV, designing a mouse IgGkappa chain signal peptide sequence at the N end of the sequence, designing a Strep tag at the C end of a peptide chain, connecting with a target protein by using a flexible Linker to obtain a modified sequence, then cloning to a lentivirus packaging vector, transfecting to 293T cells, and culturing to obtain the PEDV. And harvesting lentivirus packaging virus liquid, adding the lentivirus packaging virus liquid into CHO cells, and carrying out screening culture to obtain the CHO cell line for stably expressing the porcine epidemic diarrhea virus S1 protein. The CHO monoclonal cell strain can still express the PEDVS1 recombinant protein ...

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Номер записи: 4
25-07-2023 дата публикации

Construction and application of avian leukosis virus recombinant p27 protein genetic engineering cell line

Номер: CN116479045A
Автор: RONG FANG, LI ZUOSHENG, CHEN RUI'AI, CHEN GUI'E, SUN RONGHANG, FENG XIANING, LIU YUFU, HAO WENQIAN
Принадлежит:

The invention belongs to the technical field of genetic engineering and biological detection, and discloses construction and application of an avian leukosis virus recombinant p27 protein genetic engineering cell line. The construction method comprises the following steps: adding a Kozac sequence before an initiation codon ATG according to sequence information of an avian leukosis virus p27 protein gene, connecting a mouse IgGkappa chain signal peptide sequence to the N end of the sequence, and designing a histidine tag and a Flag tag at the C end of the sequence to obtain a modified p27 sequence; then, the modified p27 sequence is cloned to a pCMV-EGFP carrier, and a recombinant expression plasmid pCMV-EGFP-p27 is constructed and obtained; the recombinant expression plasmid is transformed to transfect CHO-K1 cells, then G418 pressurized screening is carried out to obtain monoclonal cells, and amplification culture is carried out to obtain the avian leukosis virus recombinant p27 protein ...

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Номер записи: 5
15-08-2023 дата публикации

Recombinant duck tembusu virus with E gene mutation as well as preparation method and vaccine of recombinant duck tembusu virus

Номер: CN116590241A
Автор: XIANG CHENGWEI, YANG ZEKUN, WANG TING, YANG JIE, CHEN RUI'AI
Принадлежит:

The invention relates to the technical field of molecular biology, and particularly discloses an E gene mutation recombinant duck tembusu virus and a preparation method and a vaccine thereof. According to the invention, a mutant plasmid E DID37N R166K is prepared, and then a recombinant duck tembusu virus strain rDTMUV E DID37N R166K with E gene mutation is constructed. The strain can provide a good platform for subsequent preparation of drugs and vaccines for resisting the duck tembusu virus.

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Номер записи: 6
07-04-2023 дата публикации

Saving method and application of recombinant PRRSV for expressing ASFV E183L gene

Номер: CN115927458A
Автор: CHEN RUI'AI, WEN LIANGHAI, ZHANG GUYUE, HUANG MEI, DONG NAN, YANG XIAOYUN
Принадлежит:

The invention belongs to the technical field of genetic engineering and vaccine preparation, and discloses a rescue method and application of a recombinant PRRSV (porcine reproductive and respiratory syndrome virus) expressing an ASFV E183L gene. The rescue method comprises the following steps: carrying out homologous recombination by taking amplified pBR322-CMV-line as a carrier and taking segment4A, P54 and segment4B as insertion fragments, so as to obtain a pBR322-CMV-segment4-P54 plasmid; the method comprises the following steps: by taking amplified pBR322-CMV-line as a carrier and five PCR (Polymerase Chain Reaction) amplified fragments of a PRRSV JXA1R strain genome as insertion fragments, carrying out homologous recombination to obtain pBR322-CMV-rJXA1R-P54 plasmid, and then transfecting cells for expression to obtain the recombinant PRRSV. The method is high in virus rescue success rate and can be used for developing African swine fever and other swine disease vaccines.

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Номер записи: 7
25-07-2023 дата публикации

Method for rapidly detecting salmonella based on biosensor

Номер: CN116482360A
Автор: CHU HAIPEI, CHEN RUI'AI, XIAN MEITIAN, XU FEI, LI JIAHUI, HAN YIN, FENG HAIXIA, CHEN SIXUAN
Принадлежит:

The invention is applicable to the technical field of biological detection, and provides a method for rapidly detecting salmonella based on a biosensor, which comprises the following steps: centrifuging milk to be detected at a high speed, removing fat on the upper layer and precipitate on the lower layer, adjusting the pH value of the milk, and taking the treated milk as a sample to be detected; mixing the biosensor with a to-be-detected sample, and capturing salmonella possibly existing in the to-be-detected sample; mixing the biosensor with a liquid culture medium, and culturing the obtained mixture under the condition that the salmonella can proliferate; and sucking the culture into a sterile syringe, filtering by using an ultrafiltration membrane, washing by using PBS (Phosphate Buffer Solution), observing whether residues after washing have fluorescence or not under the irradiation of an ultraviolet lamp, indicating that salmonella exists if the residues have the fluorescence, and ...

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Номер записи: 8
18-08-2023 дата публикации

Rapid IBV detection method based on LAMP and CRISPR-Cas12a

Номер: CN116606963A
Автор: LIU DINGXIANG, LIU SIYING, CHEN RUI'AI
Принадлежит:

The invention belongs to the field of biology, and discloses an IBV (infectious bronchitis virus) rapid detection method based on LAMP (loop-mediated isothermal amplification) and CRISPR-Cas12a (clustered regularly interspaced short palindromic repeats-CRISPR-Cas12a), which comprises the following steps: step 1, amplifying a target sequence of an infectious bronchitis virus through an LAMP primer group; step 2, performing secondary detection on the target sequence through crRNA matched with the target sequence, a reporter probe and Cas12a protein so as to judge whether the target sequence exists in the infectious bronchitis virus; the LAMP primer group is at least one of a first primer group, a second primer group and a third primer group; the crRNA comprises a first crRNA corresponding to the first primer group, a second crRNA corresponding to the second primer group and a third crRNA corresponding to the third primer group. According to the method, high-sensitivity and high-specificity ...

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Номер записи: 9
30-06-2023 дата публикации

RPA-LFD primer, probe and kit for detecting koi herpesvirus

Номер: CN116356080A
Автор: LI YINGYING, MO XUBING, WANG QING, YIN JIYUAN, WANG YINGYING, REN YAN, SHI CUNBIN, HU YINCHANG, CHEN RUI'AI
Принадлежит:

The invention belongs to the technical field of gene detection, and discloses an RPA-LFD primer, a probe and a kit for detecting koi herpesvirus. The invention provides the reagent for detecting the koi herpesvirus, the reagent has relatively high sensitivity and specificity, the lowest detection limit is 101 copy/[mu] L, the detection effect is stable, and the reagent has no cross reaction with the cyprinid edema virus (CEV), the cyprinid spring viraemia virus (SVCV), the cyprinid herpesvirus type II (CyHV-2) and the easily infected bacterium aeromonas hydrophila of the koi (carp).

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Номер записи: 10
09-05-2023 дата публикации

Antigen for Brucella detection as well as preparation method and application thereof

Номер: CN116083502A
Автор: CHEN RUI'AI, JIANG HUI, DING JIABO, YANG XIAOWEN, XIONG TING, LIU YUFU, LI PENG, FAN XUEZHENG, LIANG LIN, SHEN QINGCHUN
Принадлежит:

The invention relates to the technical field of animal disease detection reagents, in particular to an antigen for Brucella detection and a preparation method and application thereof. Specifically, the preparation method of the antigen comprises the following steps: (1) providing lipopolysaccharide of Brucella suis S2, and digesting the lipopolysaccharide at 1-10 DEG C for 1-30 hours by using protease K to obtain digested lipopolysaccharide; wherein the use amount of the protease K is 0.05-0.4 wt% of the lipopolysaccharide; and (2) mixing the digested lipopolysaccharide with the immune colored microspheres, and sensitizing at 1-35 DEG C for 1-30 hours to obtain an antigen finished product for Brucella detection. According to the preparation method, the purity of lipopolysaccharide is effectively improved, the specificity of the prepared antigen is improved, and specifically, the antigen can effectively distinguish positive serum such as yersinia enterocolitica O9 and escherichia coli O157 ...

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Номер записи: 11
27-06-2023 дата публикации

Porcine epidemic diarrhea virus S protein tripolymer nanoparticles and preparation method thereof

Номер: CN116333168A
Автор: JI CHUNMIAO, WANG BIN, CHEN RUI'AI, WU LIDONG, WEI JIAO
Принадлежит:

The invention discloses a porcine epidemic diarrhea virus S protein trimer nanoparticle. The nanoparticle is obtained by assembling a compound of a porcine epidemic diarrhea virus S protein trimer and I53-50A and I53-50B protein, the compound of the porcine epidemic diarrhea virus S protein trimer and the I53-50A is obtained by transfecting, expressing and purifying a first recombinant vector; the first recombinant vector comprises a first vector and a first nucleotide sequence connected to the first vector, and the first nucleotide sequence is obtained by optimizing a first amino acid sequence as shown in SEQ ID No.1 through a mammalian cell codon. The porcine epidemic diarrhea virus S protein tripolymer nanoparticle provided by the invention provides an effective way for developing a safe and efficient broad-spectrum nanoparticle vaccine aiming at PEDV (porcine epidemic diarrhea virus).

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Номер записи: 12
23-06-2023 дата публикации

Mutant duck tembusu virus strain as well as construction method and application thereof

Номер: CN116286883A
Автор: XIANG CHENGWEI, YANG ZEKUN, WANG TING, YANG JIE, CHEN RUI'AI
Принадлежит:

The invention relates to the technical field of molecular biology, and particularly discloses a mutant duck tembusu virus strain as well as a construction method and application thereof. According to the present invention, through the specific primer combination, the mutant plasmid EDII + III E89G V312A of the site-directed mutagenesis E gene is constructed, and the mutant duck tembusu virus strain rDTMUV EDII + III E89G V312A is further constructed; the strain can provide a good platform for subsequent preparation of drugs and vaccines for resisting the duck tembusu virus.

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Номер записи: 13
23-06-2023 дата публикации

Infectious bronchitis KC vaccine strain, preparation method thereof and vaccine

Номер: CN116286885A
Автор: CHEN RUI'AI, XIONG TING, LIU DINGXIANG
Принадлежит:

The invention discloses an infectious bronchitis KC vaccine strain, a preparation method thereof and a vaccine, and relates to the technical field of bioengineering. Specifically, on the basis of a specific primer, Beaudet-p65 is taken as a skeleton, amino acids at the 297th site to the 532nd site of a Beaudett (S1) coding region are replaced by S1 of a QX type prevalent virus KC strain, and other sequences are kept unchanged. And the recombinant virus rIBV-Beauu-KC (S1) is successfully rescued. A recombinant strain rIBV-Beauu-KC (S1)-p10V9 with Vero cell adaptability is obtained through continuous passage on SPF chick embryos and Vero cells, EID50 and TCID50 of the recombinant strain rIBV-Beauu-KC (S1)-p10V9 with Vero cell adaptability are 106.78/mL and 105.23/mL respectively, and the proliferation capacity of the recombinant strain rIBV-Beauu-KC (S1)-p10V9 is similar to that of a female parent virus.

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Номер записи: 14
05-05-2023 дата публикации

Recombinant adenovirus plasmid, preparation method and recombinant adenovirus

Номер: CN116064668A
Автор: CHEN RUI'AI, XU TING, XIE WENTING, WU JING
Принадлежит:

The invention belongs to the technical field of gene engineering, and discloses a recombinant adenovirus plasmid, the nucleotide sequence of which is shown as SEQNO.1. According to the plasmid and the virus based on the plasmid, P2A protein of Porcine Tescovirus 1 has a self-cleavage function, the P2A protein is used as a connecting peptide to connect VP2 of IBDV and Fiber2 protein coding genes of FAdV-4 in series, the connecting peptide is placed in an expression cassette of a replication-defective human type 5 adenovirus vector, expression of two proteins can be achieved at the same time through one-time transcription and translation, and the expression of the two proteins can be achieved at the same time. And a new thought is expected to be provided for research and development of IBD and FAdV-4 bigeminy candidate live vector vaccines. The virus can stably express VP2 and Fiber2 proteins, and the virus titer is high. Meanwhile, the invention also discloses a preparation method of the ...

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Номер записи: 15
29-09-2010 дата публикации

Recombinant adenovirus and application thereof

Номер: CN0101845419A
Автор: JUHAO CHEN, AI CHEN RUI, HONGLIANG HUANG, GENMEI LIU, MANLIN LUO, CHEN JUHAO, CHEN RUI'AI, HUANG HONGLIANG, LIU GENMEI, LUO MANLIN
Принадлежит:

The invention discloses a recombinant adenovirus, which is obtained by fusing and connecting an open reading frame 2 (ORF2) of an immunogenicity protein gene of porcine circovirus type 2 (PCV2) and a signal peptide-removed porcine interferon-gamma (IFN-gamma) gene and then recombining the fused and connected genes to an adenovirus vector. The ORF2 of the main immunogenicity protein of the PCV2 and the signal peptide-removed porcine IFN-gamma are fused with each other and then are expressed in the adenovirus, so that the immunogenicity of the ORF2 is improved and the immunity effect is enhanced compared with the single ORF2. The obtained recombined adenovirus, serving as vaccine, can be applied to epidemic prevention of the porcine circovirus.

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Номер записи: 16
11-04-2023 дата публикации

Expression preparation method of H5 subtype avian influenza virus virus-like particles

Номер: CN115948471A
Автор: CHEN RUI'AI, LI ZUOSHENG, LIU YUFU, RONG FANG, HAO WENQIAN, FENG XIANING
Принадлежит:

The invention belongs to the technical field of bioengineering and vaccine preparation, and discloses an expression preparation method of H5 subtype avian influenza virus virus-like particles. The preparation method comprises the following steps: replacing a hemagglutinin signal peptide sequence in an HA protein sequence of H5 subtype AIV with a melittin signal peptide sequence, and designing a restriction enzyme cutting site to obtain a target gene HA; a target gene HA and an M1 protein sequence are cloned to a baculovirus shuttle plasmid pFastBac1-dual through double enzyme digestion respectively, then the baculovirus shuttle plasmid pFastBac1-dual is converted to a DH5alpha competent cell to obtain a recombinant plasmid, then the recombinant plasmid is added to a DH10Bac competent cell to be cultured and extracted to obtain a recombinant baculovirus Bacmid-HA-M1, and finally the recombinant baculovirus Bacmid-HA-M1 is transfected to an SF9 insect cell to carry out VLPs expression preparation ...

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Номер записи: 17
13-06-2023 дата публикации

Recombinant adenovirus for expressing H9N2 HA protein as well as preparation method and vaccine of recombinant adenovirus

Номер: CN116254236A
Автор: XU TING, CHEN RUI'AI, LIU XIAO, LI GUIMIN, LYU YADI
Принадлежит:

The invention discloses a recombinant adenovirus for expressing H9N2HA protein as well as a preparation method and a vaccine of the recombinant adenovirus, and relates to the technical field of bioengineering. The preparation method comprises the following steps: extracting a genome of H9N2HA, and carrying out amplification and recombination, so as to obtain a recombinant shuttle plasmid pAdTrack-HA; after linearization, recombining with an adenovirus skeleton plasmid to obtain a recombinant adenovirus plasmid pAd HA; and after linearization, transfecting cells to obtain the recombinant adenovirus for expressing the H9N2HA protein. By implementing the invention, the recombinant adenovirus for expressing the H9N2 subtype avian influenza HA protein can be obtained, and a certain foundation is laid for research and development of H9N2 live virus vector vaccines.

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Номер записи: 18
07-07-2023 дата публикации

Application of atractydin in preparation of medicine for preventing or treating white spot syndrome of aquatic animals

Номер: CN116392468A
Автор: JI HUASONG, LI PENGFEI, TAKATO, CHEN RUI'AI, YU QING, HAN SHUYU, OUYANG ZHENGLIANG, CHEN BO, WANG GAOXUE, LING FEI, CHEN CHENG
Принадлежит:

The invention provides application of atractydin in preparation of a medicine for preventing or treating white spot syndrome of aquatic animals. The invention aims to explore the feasibility of atractydin in the antiviral aspect of aquaculture and expand a new way for innovative research of antiviral green fishery medicines. The invention provides an important theoretical basis for taking the atractydin as an efficient and practical anti-WSSV medicine, so that the atractydin can be expected to be developed into a medicine for preventing or treating the white spot syndrome of the aquatic animals, and a significant reference is provided for evaluating the antiviral effect and researching the action mechanism of the atractydin on other aquatic animal viruses.

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Номер записи: 19
25-04-2023 дата публикации

Application of shikimic acid in preparation of medicine for preventing and/or treating white spot syndrome of aquatic animals

Номер: CN115998723A
Автор: JI HUASONG, LI PENGFEI, TAKATO, CHEN RUI'AI, YU QING, HAN SHUYU, OUYANG ZHENGLIANG, CHEN BO, WANG GAOXUE, LING FEI, CHEN CHENG
Принадлежит:

The invention relates to application of shikimic acid in preparation of a medicine for preventing and/or treating white spot syndrome of aquatic animals. The invention provides a new medicine for preventing and treating the white spot syndrome, the replication of the white spot syndrome virus in aquatic animals can be obviously inhibited, and meanwhile, the survival rate of the aquatic animals infected by the white spot syndrome virus is increased. The shikimic acid has a good antiviral effect on the white spot syndrome virus in aquatic animal breeding, and can be applied to prevention and treatment of aquatic animal diseases caused by the white spot syndrome virus.

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Номер записи: 20
12-10-2011 дата публикации

Method for purifying swine fever viruses

Номер: CN0101974490B
Автор: XU JIAHUA, CHEN RUI'AI, TANG XIUYING, JIAHUA XU, AI CHEN RUI, XIUYING TANG
Принадлежит:

The invention discloses a method for purifying swine fever viruses from the cell sap of swine fever viruses reproduced in primary calf testicular cells, which comprises the following steps: 1) filtering the cell culture fluid of swine fever viruses; 2) concentrating the cell fluid (subjected to primary filtering) of swine fever viruses by way of transverse-cutting ultrafiltration concentration; and 3) purifying the swine fever viruses by using an anion exchange chromatography column. By using the method to purify swine fever viruses, the recovery rate of virus is 71.6 percent, the removing rate of hybrid protein is 91.06 percent, and the activity of virus has no obvious loss.

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Номер записи: 21
23-06-2023 дата публикации

Porcine epidemic diarrhea virus S protein mutant as well as preparation method and application thereof

Номер: CN116284453A
Автор: JI CHUNMIAO, WANG BIN, CHEN RUI'AI, FENG XIAOYIN
Принадлежит:

The invention relates to the technical field of biology, and particularly discloses a porcine epidemic diarrhea virus S protein mutant as well as a preparation method and application thereof. Based on in-depth analysis of the porcine epidemic diarrhea virus epidemic strain S protein, a series of mutations are carried out on the S protein, and the mutations mainly comprise mutation of cleavage sites at the joint of S1 and S2 structural domains, so that the structure of the S protein trimer is more stable; mutating amino acids at multiple sites to ensure that the S protein is in a trimer conformation state before fusion; the signal peptide is mutated into an IL-2 secretion signal peptide so as to be beneficial to S protein trimer secretion expression and the like. The porcine epidemic diarrhea virus S protein trimer obtained based on the mutation can stimulate an organism to generate stronger immune response, provides a good platform for research and development of porcine epidemic diarrhea ...

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Номер записи: 22
16-05-2023 дата публикации

Brucella monoclonal antibody 6A5 as well as application and kit thereof

Номер: CN116121199A
Автор: CHEN RUI'AI, JIANG HUI, DING JIABO, LIU YUFU, SHEN QINGCHUN, FAN XUEZHENG, XIONG TING, LIANG LIN
Принадлежит:

The invention relates to the technical field of animal disease detection, in particular to a Brucella monoclonal antibody 6A5 and application and a kit thereof. Firstly, the invention discloses a hybridoma cell strain and a Brucella monoclonal antibody 6A5 secreted by the hybridoma cell strain. The monoclonal antibody can be used for replacing bovine-derived positive serum to calibrate RBT and SAT antigens, and the monoclonal antibody is convenient to prepare and higher in specificity. Meanwhile, the technical problem that it is difficult to prepare positive serum to only contain IgG antibodies is solved, titer unification can be achieved when RBT and SAT antigens are calibrated through the high-titer IgG 1 subtype Brucella monoclonal antibody, and the quality of diagnosis antigens is improved.

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Номер записи: 23
27-06-2023 дата публикации

Saving method and application of recombinant PRRSV expressing ASFV D117L gene

Номер: CN116334133A
Автор: CHEN RUI'AI, WEN LIANGHAI, ZHANG GUYUE, HUANG MEI, DONG NAN, YANG XIAOYUN
Принадлежит:

The invention belongs to the technical field of genetic engineering and vaccine preparation, and discloses a rescue method and application of a recombinant PRRSV (porcine reproductive and respiratory syndrome virus) expressing an ASFV D117L gene. The rescue method comprises the following steps: carrying out homologous recombination by taking amplified pBR322-CMV-line as a carrier and taking segment4A, P17 and segment4B as insertion fragments, so as to obtain a pBR322-CMV-segment4-P17 plasmid; the method comprises the following steps: by taking amplified pBR322-CMV-line as a carrier and five PCR (Polymerase Chain Reaction) amplified fragments of a PRRSV JXA1R strain genome as insertion fragments, carrying out homologous recombination to obtain pBR322-CMV-rJXA1R-P17 plasmids, and then transfecting cells for expression to obtain the recombinant PRRSV. The method is high in virus rescue success rate and can be used for developing African swine fever and other swine disease vaccines.

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Номер записи: 24
04-07-2023 дата публикации

Monoclonal antibody aiming at guinea pig IFN-gamma and application thereof

Номер: CN116376845A
Автор: CHEN RUI'AI, XIN TING, DING JIABO, ZHANG GUANGZHI, FAN XUEZHENG, XIONG TING, LIU YUFU, LIANG LIN, YANG JIE
Принадлежит:

The invention relates to the technical field of medicine and animal disease detection, in particular to a monoclonal antibody aiming at guinea pig IFN-gamma, the binding specificity of the monoclonal antibody and IFN-gamma is high, a good foundation is provided for IFN-gamma determination through the monoclonal antibody, and then a good foundation is provided for tubercle bacillus titer calibration. Correspondingly, the invention also discloses a hybridoma cell strain GIFN-1A2 capable of secreting the monoclonal antibody. The invention also discloses a medicine and a kit containing the monoclonal antibody.

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Номер записи: 25
17-08-2011 дата публикации

Recombinant adenovirus and application thereof

Номер: CN0101845419B
Автор: CHEN JUHAO, CHEN RUI'AI, HUANG HONGLIANG, LIU GENMEI, LUO MANLIN, JUHAO CHEN, AI CHEN RUI, HONGLIANG HUANG, GENMEI LIU, MANLIN LUO
Принадлежит:

The invention discloses a recombinant adenovirus, which is obtained by fusing and connecting an open reading frame 2 (ORF2) of an immunogenicity protein gene of porcine circovirus type 2 (PCV2) and a signal peptide-removed porcine interferon-gamma (IFN-gamma) gene and then recombining the fused and connected genes to an adenovirus vector. The ORF2 of the main immunogenicity protein of the PCV2 and the signal peptide-removed porcine IFN-gamma are fused with each other and then are expressed in the adenovirus, so that the immunogenicity of the ORF2 is improved and the immunity effect is enhanced compared with the single ORF2. The obtained recombined adenovirus, serving as vaccine, can be applied to epidemic prevention of the porcine circovirus.

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Номер записи: 26
06-06-2023 дата публикации

VII type attenuated Newcastle disease recombinant virus for expressing H9 subtype avian influenza double-copy HA gene, and preparation method and application of VII type attenuated Newcastle disease recombinant virus

Номер: CN116218795A
Автор: CHEN RUI'AI, HUANG MEI, XIE WENTING, XU TING, YANG JIE
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The invention belongs to the field of biology, and discloses a VII type attenuated Newcastle disease recombinant virus for expressing an H9 subtype avian influenza double-copy HA gene, the virus is rDHN3mF-2HA, the rDHN3mF-HA, and the sequence of the rDHN3mF-2HA is as shown in SEQ ID NO: 1 in a sequence table; the sequence of the rDHN3mF-HA is as shown in SEQ ID NO: 2 in a sequence table. According to the Newcastle disease gene VII type attenuated strain recombinant virus rDHN3mF-2HA capable of efficiently and stably expressing HA protein of H9N2AIV, full-length membrane binding HA and another soluble HA protein with a truncated transmembrane structural domain are expressed in a non-coding region between a P gene and an M gene of an rDHN3-mF virus genome, and the Newcastle disease gene VII type attenuated strain recombinant virus rDHN3mF-2HA capable of efficiently and stably expressing the HA protein of H9N2AIV is obtained. WB verification shows that compared with an original HA gene, the ...

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Номер записи: 27
18-07-2023 дата публикации

Monoclonal antibody for resisting African swine fever virus p72 protein as well as preparation method and application thereof

Номер: CN116444654A
Автор: CHEN GUI'E, FENG XIANING, CHEN RUI'AI, RONG FANG, SUN RONGHANG, LI ZUOSHENG, LIU YUFU
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The invention belongs to the technical field of bioengineering, and discloses an anti-African swine fever virus p72 protein monoclonal antibody as well as a preparation method and application thereof. The preparation method comprises the following steps: expressing and purifying a recombinant plasmid pET28b-p72 to obtain a p72 protein immune mouse, carrying out intraperitoneal injection of p72 protein on the mouse with the highest serum antibody titer to carry out booster immunization, fusing splenocytes of the mouse subjected to booster immunization with sp2/0 cells, screening out positive hybridoma cells capable of secreting anti-p72 antibodies, and purifying the positive hybridoma cells to obtain the anti-p72 antibodies. And subcloning the positive hybridoma cell strain to obtain the monoclonal antibody cell strain for resisting the African swine fever virus p72 protein. The ASFV p72 protein is expressed by using a prokaryotic expression system, the cell strain capable of stably secreting ...

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Номер записи: 28