KOI HERPESVIRUS (KHV) ORF-149-BASED CARBON NANOTUBE SUPPORTED NUCLEIC ACID VACCINE AND APPLICATION THEREOF

Austracy The present invention discloses a koi herpesvirus (KHV) ORF149-based carbon nanotube supported nucleic acid vaccine and an application thereof. In the present invention, a single-walled carbon nanotube supported pcDNA-ORF149 nucleic acid vaccine system is successfully constructed; an antibody level of the fish is increased; and a relative percent survival is 81.9%. An immune level of the present invention is far higher than that of any other immune system. The present invention has application potentials in aquaculture and pathogen researches. Drawings of Description bp bp ...

Ultra concentrated algae bacteria microecological equipment suspension type water quality modifier and its preparation method

A suspended water improving using super-concentrated phycobacteria for microecological balance contains proportionally the concentrated seaweed liquid, concentrated bacteria liquid, special nutritive liquid and suspending agent. It has high effect to improve water quality.

Nucleic acid aptamer and Paleocystis globosa fluorescent molecule detection probe using same

The invention provides a nucleic acid aptamer and a globocystis globosa fluorescent molecule detection probe applying the same. The nucleotide sequence of the nucleic acid aptamer is shown as SEQ ID NO. 1. The nucleic acid aptamer provided by the invention has higher affinity and specificity to the phaeocystis globosa, and also has the advantages of no immunogenicity, short preparation period, good reproducibility, small molecular weight, convenience in in-vitro chemical synthesis, convenience in marking, easiness in modification and substitution of different parts of the nucleic acid aptamer, stable sequence, easiness in transportation and storage and the like. When the rapid detection method based on the nucleic acid aptamer is used for detecting the globocystis globosa, the operation is simple and rapid, and relatively high accuracy and sensitivity can be achieved. The aptamer can be used for constructing a molecular probe or a detection kit, so that the accurate detection of the Paleocystis ...

Application of sanguinarine in preparing aquatic animal medicine and method for preparing its preparation

The present invention discloses an application of sanguinarine in preparation of medicine for killing fish pathogenic bacteria and ectoparasite and preparation of its prophylactico-therapeutic preparation. Said prophylactico-therapeutic preparation is formed from sanguinarine, surfactant and solvent. Its preparation method includes the following steps: (1), making the sanguinarine be completely dissolved in a solvent with 40deg.C-50deg.C to obtain the solution containing 5%-20% of sanguinarine; and (2), making the surfactant and the solution obtained in step (1) be added into a homogenizing machine and uniformly mixed so as to obtain the invented product.

Application of isopimpinellin in preparing fishery medicine and method for preparing its preparation

The invention discloses the new use of compound isopimpinellin (C13H10O5) in the drug of killing dactylogyrus in fish external parasites, and opens up a new application field. The invention also discloses the fact that the product is a novel botanical fish drug, dactylogyrus in fish external parasites can be thoroughly killed once and for all and no drug resistance is generated. The novel botanical fish drug has rich and low-cost source of materials, simple, safe and nontoxic preparing technique, strong pharmacological action and wide market prospect of fish drug application.

Use of gracillin in medicament for killing ectoparasite of aquatic animal

The invention discloses the new application of a compound gracillin extracted and separated from plants in the medicament that can kill vitro vermin of aquatic animals, especially fish parasitoid, dactylogyrus, which proclaims the new application field. The invention also discloses a control agent which is prepared by the compound and is used for killing vitro vermin of aquatic animals and a preparation method thereof. As a non-harmful green novel fish medicine, the gracillin has low toxicity to fish and the fish is hard to generate drug resistance to gracillin, and the gracillin can thoroughly kill dactylogyrus in one time, and has the advantages of significant effect, low production cost, simple use method and convenient promotion.

Use of Fructus arctii aglycone in medicament for killing ectoparasite of aquatic animal

The invention discloses a new usage of arctigenin in insecticide for killing ectoparasites in the exterior body of an aquatic animal, in particular relates to a dactylogyrus. The invention also discloses a control agent containing the compound for killing ectoparasites in the exterior body of an aquatic animal and a preparation method thereof. As a novel green pollution-free fishery drug, the arctigenin has low toxicity to fish, is not liable to cause drug resistance, and can kill dactylogyrus thoroughly at one time, with noticeable result, low manufacturing cost, simple usage and convenient popularization.

Novel microorganism and its gene fragment order and application

A streptomycesfradiae DL2-F-5 is separated from marine or fresh water microbe beneficial bacterial group. It can produce antibacterial substances or increase immune active ingredients, prevent aquatic animal diseases, avoid destruction for purified water environment by antiseptics, inhibit pathogenic bacteria multiplying, decompose organic pollutant and residual baits in water body and improve water-body environment. It can be used in medicine, food and feed additives production.

Agent for preventing and treating aquatic animal parasitic disease

Double-shell aeromonas and application thereof as well as feed and application thereof

The invention discloses double-shell aeromonas with a collection number of CGMCC No.9137. The invention further discloses a feed. The feed contains the double-shell aeromonas and a basic nutrient substance. The invention further discloses application of the double-shell aeromonas and/or the feed in prawn culture. Besides, the invention further discloses application of the double-shell aeromonas in preparation of pawn feed. The double-shell aeromonas disclosed by the invention can secrete amylase to remarkably improve growth performances of pawn and shorten a breeding period, is safe without producing adverse effects on pawns. And moreover, by being added into the pawn feed for feeding pawns, the double-shell aeromonas disclosed by the invention can remarkably strengthen immunity of pawns.

Application of C22H34O3 for preparing chemicals for treating fish diseases, preparation and preparing method thereof

An application of C22H34O3 in preparing the fish medicine to kill the external parasite, especially dactylogyrus, is disclosed. Said fish medicine is proportionally prepared from C22H34O3, dissolvingagent, surfactant and solvent through sequential dissolving and mixing.

Bacillus cereus and application of bacillus cereus as well as feed containing bacillus cereus and application of feed

The invention discloses bacillus cereus with a collection number CGMCC (China General Microbiological Culture Collection Center) No.9139, a feed containing the bacillus cereus and basic nutrients, application of the bacillus cereus and/or the feed in prawn breeding, and application of the bacillus cereus to preparation of a prawn feed. The bacillus cereus is capable of secreting amylase, remarkably improving the growth performance of prawns and shortening the breeding cycle, is safe to the prawns and can not cause an adverse effect to the prawns. In addition, the bacillus cereus is added into the prawn feed for feeding the prawns, so that the immunity of the prawns can be remarkably improved.

Use of Fructus arctii aglycone in medicament for killing ectoparasite of aquatic animal

The invention discloses a new usage of arctigenin in insecticide for killing ectoparasites in the exterior body of an aquatic animal, in particular relates to a dactylogyrus. The invention also discloses a control agent containing the compound for killing ectoparasites in the exterior body of an aquatic animal and a preparation method thereof. As a novel green pollution-free fishery drug, the arctigenin has low toxicity to fish, is not liable to cause drug resistance, and can kill dactylogyrus thoroughly at one time, with noticeable result, low manufacturing cost, simple usage and convenient popularization.

Microorganism metabolites feed supplement cyclic decapeptide and application

The invention discloses a compound cyclic decapeptide represented by a formula (1). The compound is obtained through the fermentation and separation of a microbe Bdellovibro bacteriovorus. A plurality of experiments show that the compound can be used for preparing feed additives and medicines capable of strengthening the immunologic function and promoting animal growth. A Bdellovibro bacteriovorus fermentation crude product containing the cyclic decapeptide can be used as a feed raw material. The compound cyclic decapeptide has a wide application range.

Novel microorganism and its gene fragment order and application

Use of gracillin in medicament for killing ectoparasite of aquatic animal

The invention discloses the new application of a compound gracillin extracted and separated from plants in the medicament that can kill vitro vermin of aquatic animals, especially fish parasitoid, dactylogyrus, which proclaims the new application field. The invention also discloses a control agent which is prepared by the compound and is used for killing vitro vermin of aquatic animals and a preparation method thereof. As a non-harmful green novel fish medicine, the gracillin has low toxicity to fish and the fish is hard to generate drug resistance to gracillin, and the gracillin can thoroughly kill dactylogyrus in one time, and has the advantages of significant effect, low production cost, simple use method and convenient promotion.

MRNA vaccine for aquaculture and preparation method thereof

The invention belongs to the technical field of biological medicine, and discloses an mRNA vaccine for aquaculture and a preparation method thereof, the mRNA vaccine comprises an mRNA chain and a nano-liposome wrapping the mRNA chain; the mRNA chain is composed of a 5'cap structure, a 5 'UTR sequence, an MCP coding gene sequence, a 3' UTR sequence and a poly (A) nucleic acid sequence. The sequence of the MCP coding gene is a main capsid protein coding gene sequence of a LARGEMOUTH BASS VIRUS ISOLATE LMBV-FS001 type strain, and the nucleotide sequence of an open reading frame of the MCP coding gene sequence is shown as SEQ ID NO. 1. The mRNA vaccine can induce cellular immunity and humoral immunity at the same time, rapidly and efficiently cope with virus variation, is short in research and development and production cycle, and provides a good technical approach for prevention and control of LMBV.

Amygdalus pedunculata leaf extract and use thereof

The invention discloses an amygdalus pedunculata leaf extract, which is prepared through the following steps: drying and grinding amygdalus pedunculata leaves; performing hot reflux with 5 to 10 folds of distilled water to extract the powder for 1 to 3 times, each time for 0.5 to 2 hours; and putting the reflux extracts together and concentrating the extracts until the concentration of the raw drug is between 0.5 and 5 grams per milliliter. Amygdalus pedunculata leaf aqueous extract is used to develop and prepare insecticidal fishery medicines so as to overcome the disadvantages that chemicalfishery medicines have high toxicity and cause environmental pollution, not influence aquatic livestock to take food and grow, and be safe to the aquatic livestock.

Use of C20H27O3 in preparing fish medicine, its preparation and preparing method

The present invention discloses an application of compound C20H27O3 in preparation of medicine for killing fish ectoparasite. The composition of said medicine for killing fish ectoparasite includes (by wt%) 5%-30% of C20H27O3, 10%-20% of dissolving agent, 5%-10% of surfactant and the rest is solvent. Its preparation method includes the following steps: adding the compound C20H27O3 and dissolving agent N1N-dimethylmethanamide into a stainless steel container, fully stirring them, after they are completely dissolved, adding solvent, then adding surfactant and mixing them so as to obtain the invented product.

Method for preparing chelerythrine medicament and application thereof in prevention and treatment of bacterial diseases of aquatic animals

The invention discloses a method for preparing a chelerythrine medicament and application thereof in prevention and treatment of bacterial diseases of aquatic animals. The medicament consists of chelerythrine and starch serving as an auxiliary material. The method for preparing the prevention and treatment agent comprises the following steps of: weighing chelerythrine extract (the purity is 80 percent) and starch serving as the auxiliary material, adding the chelerythrine extract and the starch into a mixer, and mixing the chelerythrine extract and the starch uniformly to obtain a preparation containing 5 to 20 percent chelerythrine. When the bacterial disease prevention and treatment agent is added into feed for feeding, the dosage is 200 to 250 mg/kg (according to the quantity of the chelerythrine for each kilogram of weight); the prevention and treatment agent added in the feed has low toxicity, does not affect the quality of aquatic products, has no residue or pollution, is not easy ...

Application of atractydin in preparation of medicine for preventing or treating white spot syndrome of aquatic animals

The invention provides application of atractydin in preparation of a medicine for preventing or treating white spot syndrome of aquatic animals. The invention aims to explore the feasibility of atractydin in the antiviral aspect of aquaculture and expand a new way for innovative research of antiviral green fishery medicines. The invention provides an important theoretical basis for taking the atractydin as an efficient and practical anti-WSSV medicine, so that the atractydin can be expected to be developed into a medicine for preventing or treating the white spot syndrome of the aquatic animals, and a significant reference is provided for evaluating the antiviral effect and researching the action mechanism of the atractydin on other aquatic animal viruses.

Application of shikimic acid in preparation of medicine for preventing and/or treating white spot syndrome of aquatic animals

The invention relates to application of shikimic acid in preparation of a medicine for preventing and/or treating white spot syndrome of aquatic animals. The invention provides a new medicine for preventing and treating the white spot syndrome, the replication of the white spot syndrome virus in aquatic animals can be obviously inhibited, and meanwhile, the survival rate of the aquatic animals infected by the white spot syndrome virus is increased. The shikimic acid has a good antiviral effect on the white spot syndrome virus in aquatic animal breeding, and can be applied to prevention and treatment of aquatic animal diseases caused by the white spot syndrome virus.

Application of isopimpinellin in preparing fishery medicine and method for preparing its preparation

The invention discloses the new use of compound isopimpinellin (C13H10O5) in the drug of killing fish external parasites, in particular dactylogyrus and opens up a new application field. The invention also discloses the fact that the product is a novel botanical fish drug, fish external parasites can be thoroughly killed once and for all and no drug resistance is generated. The novel botanical fish drug has rich and low-cost source of materials, simple, safe and nontoxic preparing technique, strong pharmacological action and wide market prospect of fish drug application.

Immunopotentiator proline-glycocoll cyclic tetrapeptide (C14H20N404) and application

The invention relates to immunopotentiator proline-glycocoll cyclic tetrapeptide (C14H20N404). The compound is obtained by the fermentation and separation of microorganism Bacillussimplex DR-834. As shown by numerous tests, the compound can be used for preparing drugs for enhancing the immune function of animals such aquatic animal fish. A crude fermentation product of the Bacillussimplex DR-834 containing cyclic tetrapeptide can be used as feedstuff. By using the proline-glycocoll cyclic tetrapeptide, the mRNA (Messenger RNA) expression level of interleukin 1-beta gene and nitric oxide synthase in blood is obviously increased compared with contrast, and the immunity of aquatic animals can be obviously improved. The result of a virus attacking test indicates that the cyclic tetrapeptide is remarkable in immune protection. In test groups, the average death rate is 26.67%, and the rate of a contrast group is 73.33% and the relative immune protection rate of the contrast group is 63.63%.

Abalone shewanella and application thereof as well as feed and application thereof

The invention discloses abalone shewanella. The preservation number of the abalone shewanella is CGMCC No. 9138. The invention further discloses feed, wherein the feed contains the abalone shewanella and basic nutrient substances. Furthermore, the invention discloses application of the abalone shewanella and/or the feed in prawn culture. In addition, the invention additionally discloses application of the abalone shewanella in preparing prawn feed. The abalone shewanella disclosed by the invention can secrete amylase, to significantly improve growth performance of prawn and shorten a culture cycle, and the abalone shewanella is safe and free from adverse effect on the prawn. Moreover, the abalone shewanella disclosed by the invention, added to the prawn feed to feed the prawn, can significantly enhance immunity of the prawn.

Mixed bacteria agent and use thereof, and feed and use thereof

The invention discloses a mixed bacteria agent, comprising shewanella haliotis, bacillus cereus and aeromonas bivalvium, wherein the preservation number of the shewanella haliotis is CGMCC No.9138; the preservation number of the bacillus cereus is CGMCC No.9139; the preservation number of the aeromonas bivalvium is CGMCC No.9137. The invention also provides a feed containing the mixed bacteria agent and basic nutrients, use of the mixed bacteria agent and/or feed in prawn culture, and use of the mixed bacteria agent in preparation of a prawn feed. The mixed bacteria agent disclosed by the invention can secrete digestive enzymes such as protease and amylase, the digestion and absorption capacity of the prawn can be improved, the growth performance of the prawn is significantly improved, the culture cycle is shortened, and the mixed bacteria agent is safe and does not generate adverse effects on the prawns. Furthermore, the immunity of the prawns can be obviously enhanced by adding the mixed ...

Amygdalus pedunculata leaf extract and use thereof

The invention discloses an amygdalus pedunculata leaf extract, which is prepared through the following steps: drying and grinding amygdalus pedunculata leaves; performing hot reflux with 5 to 10 folds of distilled water to extract the powder for 1 to 3 times, each time for 0.5 to 2 hours; and putting the reflux extracts together and concentrating the extracts until the concentration of the raw drug is between 0.5 and 5 grams per milliliter. Amygdalus pedunculata leaf aqueous extract is used to develop and prepare insecticidal fishery medicines so as to overcome the disadvantages that chemical fishery medicines have high toxicity and cause environmental pollution, not influence aquatic livestock to take food and grow, and be safe to the aquatic livestock.

External parasite preventing and treating agent for aquatic product animals and its preparing method

The external parasite preventing and treating preparation for aquatic animals consists of cnidium fruit extract, alcohol or other solvent, and surfactant. The preparation process includes crushing cnidium fruit, reflux extraction with methanol, concentrating the extracted liquid, extraction with industrial alcohol to obtain the concentrated extracted liquid, mixing the cnidium fruit extract, surfactant and alcohol or other solvent in a homogenizer at 50-60 deg.c, cooling to normal temperature to obtain the preparation. The preparation has obvious killing effect on extracorporeal parasites of aquatic animals, no pollution, less drug resistance, low cost, and other advantages.

Agent for preventing and treating aquatic animal parasitic disease

A medicine for preventing and treating the parasite of aquatic animals is prepared from the Nicotiana rustica, sophora alopecuroides and the fruit of Brucea javanica through pulverizing separately, extracting in water for the first ingredient and alcohol extracting for the last two. It can be prepared into aqueous emulsion or dual-component form. It is diluted and sprayed on water surface when using. Its advantages are high effect and high safety.

Harpacticoida killing agent

The invention discloses a Harpacticoida killing agent. The killing agent comprises 30wt% of a Radix Polygalae extract effective component presenegenin with the content of 90% or more, 16wt% of a dispersant, 24wt% of a wetting agent and 30wt% of a carrier, the dispersant is Morwet D-400, the wetting agent is Morwet EFW, and the carrier is diatomite. The Harpacticoida killing agent has the advantages of simple and convenient operation, low toxicity to farmed animals, and one-time thorough killing of Harpacticoida.

Microorganism metabolites feed supplement cyclic decapeptide and application

The invention discloses a compound cyclic decapeptide represented by a formula (1). The compound is obtained through the fermentation and separation of a microbe Bdellovibro bacteriovorus. A plurality of experiments show that the compound can be used for preparing feed additives and medicines capable of strengthening the immunologic function and promoting animal growth. A Bdellovibro bacteriovorus fermentation crude product containing the cyclic decapeptide can be used as a feed raw material. The compound cyclic decapeptide has a wide application range.

Application of sanguinarine in preparing aquatic animal medicine and method for preparing its preparation

The present invention discloses an application of sanguinarine in preparation of medicine for killing fish pathogenic bacteria and ectoparasite and preparation of its prophylactico-therapeutic preparation. Said prophylactico-therapeutic preparation is formed from sanguinarine, surfactant and solvent. Its preparation method includes the following steps: (1), making the sanguinarine be completely dissolved in a solvent with 40deg.C-50deg.C to obtain the solution containing 5%-20% of sanguinarine; and (2), making the surfactant and the solution obtained in step (1) be added into a homogenizing machine and uniformly mixed so as to obtain the invented product.