27-09-2012 дата публикации
Номер: US20120245339A1
Автор:
Castle Linda A.,
Chen Yong Hong,
Duck Nicholas B.,
Giver Lorraine,
Gorton Rebecca,
Ivy Christina,
Kemble Roger,
McCutchen Billy Fred,
Minshull Jeremy,
Patten Phillip A.,
Siehl Dan
Принадлежит:
Novel proteins are provided herein, including proteins capable of catalyzing the acetylation of glyphosate and other structurally related proteins. Also provided are novel polynucleotides capable of encoding these proteins, compositions that include one or more of these novel proteins and/or polynucleotides, recombinant cells and transgenic plants comprising these novel compounds, diversification methods involving the novel compounds, and methods of using the compounds. Some of the novel methods and compounds provided herein can be used to render an organism, such as a plant, resistant to glyphosate. 1. An isolated or recombinant polynucleotide comprising:(a) a nucleotide sequence encoding an amino acid sequence that can be optimally aligned with a sequence selected from the group consisting of SEQ ID NO:300, SEQ ID NO:445 and SEQ ID NO:457 to generate a similarity score of at least 460, using the BLOSUM62 matrix, a gap existence penalty of 11, and a gap extension penalty of 1, (i) at positions 18 and 38, a Z5 amino acid residue;', '(ii) at position 62, a Z1 amino acid residue;', '(iii) at position 124, a Z6 amino acid residue; and', '(iv) at position 144, a Z2 amino acid residue,, 'wherein the amino acid sequence comprises one or more amino acid residues which meet at least one restriction selected from the group consisting ofwherein:Z1 is an amino acid residue selected from the group consisting of A, I, L, M, and V;Z2 is an amino acid residue selected from the group consisting of F, W, and Y;Z5 is an amino acid residue selected from the group consisting of D and E;Z6 is an amino acid residue selected from the group consisting of C, G, and P; or(b) a complementary nucleotide sequence thereof.2. The isolated or recombinant polynucleotide of claim 1 , wherein of the amino acid residues in the amino acid sequence that correspond to the following positions claim 1 , at least 90% conform to the following restrictions:(a) at positions 2, 4, 15, 19, 26, 28, 31, 45, 51, 54 ...
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