11-02-2009 дата публикации
Номер: CN0101363063A
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The invention discloses a primer, a probe and a detection kit containing the primer and the probe, which are used for detecting A type, B type and H5 subtype influenza viruses through triple fluorescence quantitative RT-PCR. The invention also discloses a method for executing fluorescence quantitative RT-PCR for A type, B type and H5 subtype influenza viruses by using the primer and the probe. The detection kit includes RT-PCR reaction buffer, Taq enzyme, inverse transcriptase, DEPC liquid, positive control and negative control. The process involves the steps of establishing a RT-PCR reaction system by using the primer and the probe, extracting DNA or RNA from a specimen to be detected, adding the extracted DNA or RNA into the aforesaid reaction system, setting reaction conditions, carrying out RT-PCR augmentation and fluorescence signal detection. The invention can identify A type, B type and H5 subtype influenza viruses in the detected specimen within 3-4 hours in a fast, exact, specific ...
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