07-09-2017 дата публикации
Номер: US20170253916A1
The disclosure provides a method for diagnosing an active infection by detecting certain RNA biomarkers present in secreted extracellular vesicles isolated from a bodily fluid. The RNA biomarkers in the secreted extracellular vesicles may include a certain RNAs as well as certain host cell RNAs. Also provided is an RNA signature of certain and host cell RNA present in secreted extracellular vesicles indicative of an active tuberculosis infection. 1M. tuberculosis. A method for identifying an active infection in a subject comprising:isolating extracellular vesicles from bodily fluid of a subject, wherein the extracellular vesicles contain a plurality of RNA;extracting at least a portion of the RNA from the extracellular vesicle; and{'i': M. tuberculosis', 'M. tuberculosis, 'analyzing the RNA for the presence of one or more RNA that are indicative of an active infection, thereby determining the presence or absence of an active infection in the subject.'}2M. tuberculosis. The method of claim 1 , wherein the RNA that are indicative of an active infection is selected from the group consisting of RV1821 claim 1 , RV1842c claim 1 , RV3894c claim 1 , RV0453 claim 1 , RV1629 claim 1 , RV0170 claim 1 , RV0668 claim 1 , RV0740 claim 1 , RV0288 claim 1 , RV1344 claim 1 , RV0968 claim 1 , RV1942c claim 1 , RV0664 claim 1 , RV0190 claim 1 , RV1757c claim 1 , RV1369c claim 1 , RV3809c claim 1 , RV3533 claim 1 , RV0243 claim 1 , RV1101c claim 1 , and RV2024c.3M. tuberculosis. The method of claim 1 , wherein the RNA that are indicative of an active infection is selected from the group consisting of 149-3p claim 1 , 181c-5p claim 1 , 1839-3p claim 1 , 151-3p claim 1 , 214-3p claim 1 , 292-3p claim 1 , 3107-5p claim 1 , 3074-5p claim 1 , 344i claim 1 , 486-5p claim 1 , 486-3p claim 1 , 434-5p claim 1 , 598-3p claim 1 , 5099 claim 1 , 5113 claim 1 , 5106 claim 1 , 5097 claim 1 , 5621-5p claim 1 , 5115 claim 1 , 5111-5p claim 1 , 714 claim 1 , 877-3p claim 1 , 759 and 713.4. The method ...
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