23-06-2023 дата публикации
Номер: CN116287448A
Принадлежит:
The invention belongs to the technical field of virus detection, and particularly relates to an RAA primer pair and a probe for detecting African swine fever virus and application of the RAA primer pair and the probe. The RAA primer pair comprises an upstream primer with a sequence as shown in SEQ ID NO.1 and a downstream primer with a sequence as shown in SEQ ID NO.2, and the probe comprises a sequence as shown in SEQ ID NO.3. When the RAA primer pair and the probe for detecting the African swine fever virus are used for detecting the African swine fever virus, ASFV amplification can be completed within 10-20 min, ASF detection can be completed within 15-25 min (amplification and color development), and special instruments and equipment are not needed; the kit has the sensitivity of 48.75 copies/reaction, has no cross reaction with common viruses in pig farms, has the detection result coincidence rate of 100% with an ASFV qPCR kit, and can be used for on-site rapid detection of ASF.
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