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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 15. Отображено 15.
15-07-2009 дата публикации

High-voltage pulse electric field inactivation method for Clostridium welchii and inspection method thereof

Номер: CN0101481684A
Автор: ZHOU JIZHANG, GONG XIAOWEI, ZHENG FUYING, CAO XIAOAN, LIN GUOZHEN, QIU CHANGQING, JIZHANG ZHOU, XIAOWEI GONG, FUYING ZHENG, XIAOAN CAO, GUOZHEN LIN, CHANGQING QIU
Принадлежит:

The invention relates to an inactivating method and a testing method for clostridium welchii. The inactivating method comprises steps of treating bacterial liquid with pulse electric field having electric field intensity of 32-50KV/cm and pulse frequency of 500Hz-2,000Hz at a temperature of between 30 and 55 DEG C, and collecting the treated bacterial liquid to obtain the inactivated bacterial liquid. The testing method comprises steps of inoculating the inactivated bacterial liquid in anaerobic liver soup or a fresh blood dish to culture and then testing with a microscope. The treated product and the whole inactivating process contacted by processors do not generate any side effect likely after formaldehyde is treated in the prior art.

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Номер записи: 1
19-01-2011 дата публикации

Kit and method for detecting riemerella anatipestifer

Номер: CN0101948929A
Автор: FUYING ZHENG, CHANGQING QIU, GUOZHEN LIN, JIZHANG ZHOU, XIAOAN CAO, XIAOWEI GONG, ZHENG FUYING, QIU CHANGQING, LIN GUOZHEN, ZHOU JIZHANG, CAO XIAOAN, GONG XIAOWEI
Принадлежит:

The invention discloses a kit for detecting riemerella anatipestifer and a method for using the kit. At least two pairs of specific primers F3, B3, FIP and BIP for loop-mediated isothermal amplification are placed in the kit for detecting riemerella anatipestifer. For making use convenient, the kit also comprises lycine, magnesium sulfate, dNTPs, ThermoPol reaction buffer and Bst DNA polymerase; or a color developing reagent SYBR GREEN I is also added. The kit and the method have the advantages of convenient application on base layer, short detection time, low cost and simple and convenient operation.

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Номер записи: 2
18-11-2009 дата публикации

Kit used for detecting bovine abortus Chlamydia psittaci antibody and preparation method thereof

Номер: CN0101581723A
Автор: QIU CHANGQING, SONG ZHUQING, ZHOU JIZHANG, CAO XIAOAN, ZHENG FUYING, LIN GUOZHEN, GONG XIAOWEI, CHANGQING QIU, ZHUQING SONG, JIZHANG ZHOU, XIAOAN CAO, FUYING ZHENG, GUOZHEN LIN, XIAOWEI GONG
Принадлежит:

The invention discloses an ELISA kit used for detecting a bovine abortus Chlamydia psittaci antibody and a preparation method thereof. The ELISA kit comprises a horizontally coated abortus Chlamydia psittaci, an ELIAS plate longitudinally coated with rabbit anti-bovine IgG-HRP as a second antibody, a washing liquid, a confining liquid, a substrate buffer solution A, a substrate buffer solution B and a stop solution, wherein the coated abortus Chlamydia psittaci antigen is abortus Chlamydia psittaci outer membrane protein OmpA, especially the recombinant protein of the abortus Chlamydia psittaci outer membrane protein OmpA.

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Номер записи: 3
01-12-2010 дата публикации

Kit for detecting bovine epizootic fever virus and related method

Номер: CN0101899533A
Автор: FUYING ZHENG, CHANGQING QIU, GUOZHEN LIN, JIZHANG ZHOU, XIAOAN CAO, XIAOWEI GONG, ZHENG FUYING, QIU CHANGQING, LIN GUOZHEN, ZHOU JIZHANG, CAO XIAOAN, GONG XIAOWEI
Принадлежит:

The invention discloses a kit for detecting bovine epizootic fever virus and a using method thereof. The detection kit has at least two pairs of specific primers F3, B3, FIP and BIP for loop-mediated isothermal amplification. The method is used for detecting by using the loop-mediated isothermal nucleic acid amplification technology. The method has the advantages of no need of thermal cycle reaction, no need of amplification in a PCR instrument, simple equipment and low time consumption.

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Номер записи: 4
16-06-2010 дата публикации

Method for preparing sustained-release polycarboxylic water reducer

Номер: CN0101735411A
Автор: CUI SUPING, LAN MINGZHANG, LI HUIQUN, LIU XIAO, WANG YALI, WANG ZIMING, ZHENG FUYING, SUPING CUI, MINGZHANG LAN, HUIQUN LI, XIAO LIU, YALI WANG, ZIMING WANG, FUYING ZHENG
Принадлежит:

The invention discloses a method for preparing a sustained-release polycarboxylic water reducer and belongs to the field of water reducers. The water reducer is prepared by the following steps: adding a macromonomer of a polyoxyethylene ether or ester containing an unsaturated double bond, an unsaturated sulfonic acid or a salt monomer a thereof, a mono-olefin carboxylic acid derivative b, a diolefin carboxylic acid derivative c and water to a reaction kettle; reacting at the temperature of between 60 and 85 DEG C for 1.0 to 6.0 hours under the action of persulfate of an initiator; and reducing the temperature to 40-55 DEG C, and neutralizing the mixture to a pH between 6 and 8 by using alkaline solution. The method for preparing the sustained-release polycarboxylic water reducer introduces the mono-olefin carboxylic acid derivative b and the diolefin carboxylic acid derivative c into a main chain of polycarboxylic acid, so that the prepared polycarboxylic water reducer has sustained-release ...

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Номер записи: 5
09-03-2011 дата публикации

Brucella diagnostic kit and use method thereof

Номер: CN0101984068A
Автор: GUOZHEN LIN, CHANGQING QIU, FUYING ZHENG, JIZHANG ZHOU, XIAOAN CAO, XIAOWEI GONG, LIN GUOZHEN, QIU CHANGQING, ZHENG FUYING, ZHOU JIZHANG, CAO XIAOAN, GONG XIAOWEI
Принадлежит:

The invention discloses a microbiological detection technology, in particular to a kit for diagnostic detection of Brucella and a use method of the kit. The Brucella detection kit of the invention at least comprises two pairs of specific amplification primers. The invention has the principle that by utilizing the loop-mediated isothermal amplification reaction (LAMP reaction for short), Bst DNA polymerase and two pairs of specific inner and outer primers (inner primers FIP/BIP and outer primers F3/B3) designed according to a target gene sequence specifically recognize six independent regions on the target sequence, start cyclic chain displacement reaction and start complementary chain synthesis in the target DNA region, so that on the same chain, a complementary sequence cyclically forms a stem-loop DNA mixture with a plurality of loops and a cauliflower structure.

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Номер записи: 6
16-02-2011 дата публикации

Normal-temperature synthesis method for polycarboxylic acid water-reducing agent

Номер: CN0101974135A
Автор: ZIMING WANG, YING XU, HAO WU, XIAO LIU, FUYING ZHENG, HUIQUN LI, SUPING CUI, MINGZHANG LAN, YALI WANG, WANG ZIMING, XU YING, WU HAO, LIU XIAO, ZHENG FUYING, LI HUIQUN, CUI SUPING, LAN MINGZHANG, WANG YALI
Принадлежит:

The invention discloses a normal-temperature synthesis method for a polycarboxylic acid water-reducing agent, and belongs to the field of cement concrete water-reducing agents. The water-reducing agent is prepared by the following steps of: copolymerizing polyoxyethylene ether monomer or polyoxyethylene ester monomer a containing unsaturated double bonds, unsaturated monocarboxylic acid and derivative monomer thereof b, unsaturated dicarboxylic acid c and unsaturated sulfonic acid or salt monomer thereof d in aqueous solution under the action of an oxidation reduction initiator, and finally neutralizing the solution by using alkali solution to obtain the water-reducing agent. The reaction can be performed at room temperature by adopting an oxidation reduction initiating system, and the appropriate reaction temperature is between 5 and 30 DEG C; and the synthesis process does not need heating, so energy is saved, and high-temperature side reaction is effectively controlled. The prepared ...

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Номер записи: 7
18-11-2009 дата публикации

Abortus Chlamydia psittaci vaccine and preparation method thereof

Номер: CN0101579521A
Автор: QIU CHANGQING, SONG ZHUQING, ZHOU JIZHANG, CAO XIAOAN, ZHENG FUYING, LIN GUOZHEN, GONG XIAOWEI, CHANGQING QIU, ZHUQING SONG, JIZHANG ZHOU, XIAOAN CAO, FUYING ZHENG, GUOZHEN LIN, XIAOWEI GONG
Принадлежит:

The invention relates to a bovine abortus Chlamydia psittaci subunit vaccine and a preparation method thereof. The vaccine is the subunit vaccine formed by Chlamydophila abortus outer membrane protein A (OmpA), and the preparation method of the vaccine comprises the steps of extracting bacterial total DNA to be served as a template from the Chlamydophila abortus, designing two pairs of specific primers and conducting nested polymerase chain reaction and amplification to obtain the complete gene of the Chlamydophila abortus outer membrane protein A (OmpA), and cloning the gene to a prokaryotic expression vector to obtain a recombinant expression vector; using the recombinant expression vector to transform colon bacillus competent cells, extracting recombinant plasmid and screening out positive clone by PCR and enzyme cutting identification; and transferring the recombinant plasmid into the colon bacillus and conducting induction expression to obtain the antigen protein used for the vaccine ...

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Номер записи: 8
15-06-2011 дата публикации

Gas washing and recovering device for preparing macromonomers of poly carboxylic acid water reducing agent

Номер: CN0102091508A
Автор: ZIMING WANG, FUYING ZHENG, YING XU, HUIQUN LI, HAO WU, TAOAN LI, XIAO LIU, SUPING CUI, MINGZHANG LAN, YALI WANG, QIANJIN MAO, WANG ZIMING, ZHENG FUYING, XU YING, LI HUIQUN, WU HAO, LI TAOAN, LIU XIAO, CUI SUPING, LAN MINGZHANG, WANG YALI, MAO QIANJIN
Принадлежит:

The invention discloses a gas washing and recovering device for preparing macromonomers of a poly carboxylic acid water reducing agent and relates to the field of a gas washing device for synthetic resin. The device comprises a mixed gas inlet pipe, a coil pipe (which is formed by winding a pipeline into a circle), a water inlet pipe, an emptying pipe, a charging hopper, a grating and a ceramic ball or a glass ball. The mixed gas inlet pipe enters from the top of the recovering device and is connected with the coil pipe at the bottom of the recovering device. The coil pipe is provided with small holes with different sizes at equal distances. The diameters of the small holes are gradually increased from a gas inlet end to a far end. The small holes are positioned at the lower part of the coil pipe. The grating positioned above the coil pipe is provided with the ceramic ball or the glass ball. Gas is uniformly exhausted from the coil pipe. After the gas is in sufficient contact with a solvent ...

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Номер записи: 9
23-12-2009 дата публикации

brucella vaccine and method for preparing antigen protein used for same

Номер: CN0101607081A
Автор: CAO XIAOAN, QIU CHANGQING, ZHOU JIZHANG, ZHENG FUYING, LIN GUOZHEN, GONG XIAOWEI, XIAOAN CAO, CHANGQING QIU, JIZHANG ZHOU, FUYING ZHENG, GUOZHEN LIN, XIAOWEI GONG
Принадлежит:

The invention discloses a brucella vaccine applied to livestock and a method for preparing antigen protein used for same. The antigen protein used for the brucella vaccine is brucella bcsap31 protein, and in particular brucella bcsp31 restructured protein. The method for preparing the restructured protein has steps of: taking bacterium total DNA extracted from the brucella as a template, designing a pair of primers according to ribotide complete sequence of brucella bcsp31 gene to carry out polymerase chain reaction, obtaining bcsp31 protein gene by clone and then transferring the bcsp31 protein gene to a yeast expression vector to obtain a restructured expression vector, importing the obtained restructured expression vector to yeast expression bacterium to obtain restructured pichia pastoris expression bacterium, and carrying out proliferation and induced expression of the restructured pichia pastoris expression bacterium so as to obtain the required antigen protein by separation and purification ...

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Номер записи: 10
04-03-2010 дата публикации

ENCLOSED MEMBRANE-CLAMPING DEVICES FOR RUNNING BIOLOGICAL ASSAYS ON MEMBRANE SURFACES

Номер: US20100055664A1
Автор: FUYING ZHENG, YIQI LUO, ZHENG FUYING, LUO YIQI
Принадлежит:

An enclosed membrane-clamping (EMC) device is disclosed for running biological assays on membrane surfaces, such as Western blotting. The EMC device comprises a cover plate and a support plate, which can be coupled through a sealing mechanism. The cover plate, the support plate and the sealing mechanism are shaped such that their inner surfaces form one or more enclosed chambers. When in use, a blotting membrane is placed between the cover plate and the support plate, and clamped in the chamber formed in the EMC device. The EMC device is coupled with an assisting device to realize automation of manipulations. Liquid-phase target solutions are introduced into the chamber through its inlet and outlet to realize surface-molecular interactions between the target on the blotting membrane and the targets introduced in liquid phase. The chamber with a small vertical dimension is capable of achieving the blotting assays at a higher speed.

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Номер записи: 11
20-06-2012 дата публикации

Kit and method for detecting riemerella anatipestifer

Номер: CN0101948929B
Автор: FUYING ZHENG, CHANGQING QIU, GUOZHEN LIN, JIZHANG ZHOU, XIAOAN CAO, XIAOWEI GONG, ZHENG FUYING, QIU CHANGQING, LIN GUOZHEN, ZHOU JIZHANG, CAO XIAOAN, GONG XIAOWEI
Принадлежит:

The invention discloses a kit for detecting riemerella anatipestifer and a method for using the kit. At least two pairs of specific primers F3, B3, FIP and BIP for loop-mediated isothermal amplification are placed in the kit for detecting riemerella anatipestifer. For making use convenient, the kit also comprises lycine, magnesium sulfate, dNTPs, ThermoPol reaction buffer and Bst DNA polymerase; or a color developing reagent SYBR GREEN I is also added. The kit and the method have the advantages of convenient application on base layer, short detection time, low cost and simple and convenient operation.

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Номер записи: 12
10-08-2011 дата публикации

Method for preparing sustained-release polycarboxylic water reducer

Номер: CN0101735411B
Автор: CUI SUPING, LAN MINGZHANG, LI HUIQUN, LIU XIAO, WANG YALI, WANG ZIMING, ZHENG FUYING, SUPING CUI, MINGZHANG LAN, HUIQUN LI, XIAO LIU, YALI WANG, ZIMING WANG, FUYING ZHENG
Принадлежит:

The invention discloses a method for preparing a sustained-release polycarboxylic water reducer and belongs to the field of water reducers. The water reducer is prepared by the following steps: adding a macromonomer of a polyoxyethylene ether or ester containing an unsaturated double bond, an unsaturated sulfonic acid or a salt monomer a thereof, a mono-olefin carboxylic acid derivative b, a diolefin carboxylic acid derivative c and water to a reaction kettle; reacting at the temperature of between 60 and 85 DEG C for 1.0 to 6.0 hours under the action of persulfate of an initiator; and reducing the temperature to 40-55 DEG C, and neutralizing the mixture to a pH between 6 and 8 by using alkaline solution. The method for preparing the sustained-release polycarboxylic water reducer introduces the mono-olefin carboxylic acid derivative b and the diolefin carboxylic acid derivative c into a main chain of polycarboxylic acid, so that the prepared polycarboxylic water reducer has sustained-release ...

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Номер записи: 13
10-11-2010 дата публикации

Hog cholera indirect hemagglutination detection kit and preparation

Номер: CN0101487017B
Автор: GONG XIAOWEI, LIN GUOZHEN, ZHOU JIZHANG, ZHENG FUYING, CAO XIAOAN, QIU CHANGQING, XIAOWEI GONG, GUOZHEN LIN, JIZHANG ZHOU, FUYING ZHENG, XIAOAN CAO, CHANGQING QIU
Принадлежит:

The invention relates to a preparation method of a recombinant protein, an indirect classical swine fever hemagglutination test kit that is prepared by the protein, and a preparation method of the test kit. The preparation method of the protein comprises following steps: a segment with the gene homeology degree comparing with a bovine viral diarrhea-mucosal disease virus (BVDV) that is lower than35 percent is selected from a conservative region of a classical swine fever virus (CSFV) gene, thus obtaining a nucleotide sequence S1 that is corresponding to an amino acid A1; a forth amino acid in the nucleotide sequence S1 is modified to an hydrophilic amino acid, thus obtaining an amino acid A2; a corresponding nucleotide sequence S2 of A2 is modified, thus respectively obtaining three new nucleotide sequences; the three new nucleotide sequences are orderly and serially connected by connecting nucleotides, thus obtaining a sequence S9 that is corresponding to an amino acid sequence A3; and ...

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Номер записи: 14
22-07-2009 дата публикации

Hog cholera indirect hemagglutination detection kit and preparation

Номер: CN0101487017A
Автор: ZHENG FUYING, QIU CHANGQING, LIN GUOZHEN, ZHOU JIZHANG, CAO XIAOAN, GONG XIAOWEI, FUYING ZHENG, CHANGQING QIU, GUOZHEN LIN, JIZHANG ZHOU, XIAOAN CAO, XIAOWEI GONG
Принадлежит:

The invention relates to a preparation method of a recombinant protein, an indirect classical swine fever hemagglutination test kit that is prepared by the protein, and a preparation method of the test kit. The preparation method of the protein comprises following steps: a segment with the gene homeology degree comparing with a bovine viral diarrhea-mucosal disease virus (BVDV) that is lower than 35 percent is selected from a conservative region of a classical swine fever virus (CSFV) gene, thus obtaining a nucleotide sequence S1 that is corresponding to an amino acid A1; a forth amino acid in the nucleotide sequence S1 is modified to an hydrophilic amino acid, thus obtaining an amino acid A2; a corresponding nucleotide sequence S2 of A2 is modified, thus respectively obtaining three new nucleotide sequences; the three new nucleotide sequences are orderly and serially connected by connecting nucleotides, thus obtaining a sequence S9 that is corresponding to an amino acid sequence A3; and ...

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Номер записи: 15