Water-insoluble preparations of peptide materials, their production and their use

Abstract of the Disclosure There are disclosed water-insoluble proteinic preparations in which a protein or polypeptide is bound to a copolymer, which preparations are useful for binding enzymes in order to carry out enzyme-catalyzable reactions. These preparations comprise a peptide material bound to a cross-linked copolymer comprising the following copolymerized units: (A) about 0.1 to 30 wt. % of at least one .alpha.,.beta.-monoolefinically unsaturated dicarboxylic acid anhydride having about 4 to 9 carbon atoms; (B) about 35 to 90 wt. % of at least one di- and/or poly(meth)acrylate of a diol and/or polyol as hereinafter de-fined; and (C) about 5 to 60 wt. % of at least one hydrophilic monomer, the copolymer having a bulk volume of 1.4 to 30 ml/g and a specific surface area of about 1 to 500 m2/g, and containing, after saponification of the anhydride groups, about 0.01 to 14 milliequivalents of acid per gram.

Penicillin acylase

Production of 6-aminopenicillanic acid

An extremely important major industrial advance in the field of manufacture of 6-aminopenicillanic acid is disclosed which involves contacting a penicillin and a water-insoluble polymeric carrier-bound penicillin acylase with the concommitant production of 6-aminopenicillanic acid.

Enzymatic production of 6-aminopenicillanic acid