22-05-2013 дата публикации
Номер: CN103109747A
Принадлежит:
The invention relates to a plant propagation technology, and particularly relates to a rapid pseudolarix propagation method based on stem node propagation. The rapid pseudolarix propagation method comprises the following steps of: 1, picking a newly germinated immature stem on a pseudolarix seedling trunk to be used as an explant; 2, inoculating to a basic culture medium, adding 0.02-0.04mg.L<-1> of indoleacetic acid, 0.03-0.06mg.L<-1> of naphthylacetic acid, 0.10-0.12mg.L<-1> of 6-benayl aminopurine and 2.70-2.80mg.L<-1> of gibberellin for rooting the immature stem of the pseudolarix and germinating an axillary bud, growing and culturing; and 3, carrying out rapid propagation, acclimatization and transplant on the pseudolarix plant. A tender stem tip on the pseudolarix wild seedling trunk is used as a material, and the stem is induced in a test tube to root and the axillary bud germinates and grows, and thus one-step rapid propagation of stem node reduplication is achieved, and high-quality ...
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