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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 25. Отображено 25.
22-05-2013 дата публикации

Pollen liquid for high yield of hazelnut fruit and cultivation and pollination of hybrid hazel variety, and preparation method thereof

Номер: CN103109737A
Принадлежит:

The invention relates to a preparation method of pollen liquid for high yield of hazelnut fruit and cultivation and pollination of hybrid hazel variety, and belongs to a method for high yield of plant fruit and variety cultivation. The pollen liquid is prepared from the following raw materials by weight: 38.0g of mixed pollen, 7.0-8.5mg of boric acid, 5.0-6.5mg of calcium chloride dehydrate, 38.0-42.0g of polyethylene glycol 6000, and 55g of cane sugar. The preparation method comprises the following steps of: evenly mixing and then fixing volume to 1000mL by distilled water as pollen mother liquor for use, wherein the mixed pollen is that the ratio of hazel pollen to mandshurica batal to flat yellow pollen is 1 to 1 to 2; taking 200mL of pollen mother liquor when the pollen liquid is used for producing the hazel; vibrating, cultivating and diluting to 2000mL, adjusting the pH to 5.8-6.5; and evenly spraying female flower for producing filbert to finish pollination. The pollen spraying liquid ...

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22-05-2013 дата публикации

Method for removing tobacco mosaic virus and rapidly cultivating non-toxic seedling in test tube

Номер: CN103109745A
Принадлежит:

The invention relates to a method for removing a tobacco mosaic virus and rapidly cultivating a non-toxic seedling in a test tube. The method comprises the following steps: (1) seeding the seeds of flue-cured type tobacco into a seedling tray, wherein treated stem tips after the seeds sprout are used as explants for standby; (2) inoculating the stem tips to a minimal medium added with 0.02-0.05mg.L of indoleacetic acid, 1.50-1.70mg.L of gibberellins, 10g.L of edible sugar and 8.5g.L of agar stripes for cultivating to remove a tobacco mosaic virus; (3) controlling the cultivating temperature to be 52-54 DEG C, and cultivating for 48-54 days, thus achieving the removal of the tobacco mosaic virus; and (4) when the stem tip grows to 1.0cm, cutting 0.05cm of stem tip to further carry out nontoxic seedling cultivation. The method for removing the tobacco mosaic virus and rapidly cultivating the non-toxic seedling in the test tube can be directly applied to industrialized production of vitis ...

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20-03-2013 дата публикации

Rapid adonis amurensis seed after-ripening method

Номер: CN102972123A
Принадлежит:

The invention relates to a seed breeding method, and particularly relates to a rapid adonis amurensis seed after-ripening method. The after-ripening method comprise the following steps of: drying adonis amurensis aggregate fruit balls, removing seed coats of the aggregate fruit balls, and immersing the aggregate fruit balls in a mixed solution of 2.50-3.00 mg/L 2,4-dichlorphenoxyacetic acid and 0.60-0.70 mg/L 6-benzylaminopurine for 48h; and uniformly mixing seeds with river sand, and carrying out temperature changing treatment on the mixture in a wooden tank indoors for 88-102 days, wherein the wooden tank has a good ventilation property and is covered with a plastic film, thus finally developing the seeds into mature cotyledon embryo seeds. The temperature changing amplitude and time of the temperature-changing treatment are that the daytime temperature is 20+/-2 DEG C and the treatment lasts for 12h, and the night temperature is 10+/-2 DEG C and the treatment lasts for 12h; and the mixing ...

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22-05-2013 дата публикации

Rapid pseudolarix propagation method based on stem node propagation

Номер: CN103109747A
Принадлежит:

The invention relates to a plant propagation technology, and particularly relates to a rapid pseudolarix propagation method based on stem node propagation. The rapid pseudolarix propagation method comprises the following steps of: 1, picking a newly germinated immature stem on a pseudolarix seedling trunk to be used as an explant; 2, inoculating to a basic culture medium, adding 0.02-0.04mg.L<-1> of indoleacetic acid, 0.03-0.06mg.L<-1> of naphthylacetic acid, 0.10-0.12mg.L<-1> of 6-benayl aminopurine and 2.70-2.80mg.L<-1> of gibberellin for rooting the immature stem of the pseudolarix and germinating an axillary bud, growing and culturing; and 3, carrying out rapid propagation, acclimatization and transplant on the pseudolarix plant. A tender stem tip on the pseudolarix wild seedling trunk is used as a material, and the stem is induced in a test tube to root and the axillary bud germinates and grows, and thus one-step rapid propagation of stem node reduplication is achieved, and high-quality ...

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22-05-2013 дата публикации

Direct umbrellaleaf regeneration seedling-raising method based on radical bud

Номер: CN103109748A
Принадлежит:

The invention relates to a plant propagation technology, and particularly relates to a direct umbrellaleaf direct regeneration seedling-raising method based on a radical bud. The method comprises the following steps of: 1, treating an umbrellaleaf radical bud to be used as an explant for later use; 2, inducing and rooting multiple shoots of the umbrellaleaf radical bud, to be specific, adding 0.02-0.03mg.L<-1> of naphthylacetic acid, 0.15mg.L<-1> of 6-benayl aminopurine, 0.50-0.60mg.L<-1> of kinetin, 2.20-2.40mg.L<-1> of gibberellin in a basic culture medium; and 3, rapidly propagating a umbrellaleaf sprout. The direct umbrellaleaf regeneration seedling-raising method is used for rapidly propagating umbrellaleaf by using a mode of inducing the radical bud to directly regenerate the multiple shoots and rooting, and can be applied to factorized propagation of the umbrellaleaf sprout and the large-scale cultivation and development and utilization of the umbrellaleaf. The umbrellaleaf radical ...

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20-03-2013 дата публикации

One-step rapid seedling method for tender stem segment of malus komarovii inside test tube

Номер: CN102972292A
Принадлежит:

The invention relates to plant propagation, namely a one-step rapid seedling method for a tender stem segment of malus komarovii inside a test tube. The method comprises the steps of (1) collecting branches of the malus komarovii in the last ten days of October, carrying out dormancy at low temperature; promoting a dormant bud to germinate by water culture, and cutting the processed branches into segments, each of which contains a leaf, as explants for use; (2) enabling the malus komarovii to root, wherein the culture medium for growth of an axillary bud of the stem segment is culture medium+6-BA (0.07-0.09mg.L-1)+IBA (0.02-0.04mg.L-1)+GA3(0.09-0.13mg.L-1). By adopting the one-step rapid seeding method disclosed by the invention, rooting of the tender stem segment of the malus komarovii inside the test tube is facilitated, and germination of the axillary bud is facilitated at the same time; the method is high in rooting percentage, rapid in germination speed of the axillary bud, and strong ...

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17-04-2013 дата публикации

Year-round strawberry hybridizing and breeding method

Номер: CN103039356A
Принадлежит:

The invention relates to plant propagation, and in particular relates to a year-round strawberry hybridizing and breeding method which comprises the following step of: taking fragaria orientalis and rupee strawberry test tube seedlings as test materials. A regulating and controlling method for male sterile line of the rupee strawberry is as follows: selecting a plurality of runner tips of the rupee strawberry test tube seedling, controlling the temperature at 10-13 DEG C, controlling illuminating hour as 4-5h.d<-1>, and processing for 16-25 days, to promote the rupee strawberry to generate the male sterile line; and a method for preparing fragaria orientalis pollen mother liquid comprises the following steps of: setting volume of distilled water mixing liquid of 5.2g of fragaria orientalis pollen and boric acid, 150g of sucrose, 12.5mg of calcium chloride dihydrate and 36g of polyethylene glycol 4000 to 1L, and using the distilled water mixing liquid as mother liquid for further, wherein ...

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13-03-2013 дата публикации

Method for efficiently forming seedlings in vitro by utilizing stem segments of rhododendron plants in Changbai Mountains

Номер: CN102960250A
Принадлежит:

The invention relates to plant propagation and in particular relates to a method for efficiently forming seedlings in vitro by utilizing stem segments of rhododendron plants in the Changbai Mountains. Taking rhododendron aureum as example, the method comprises the steps of: (1) collecting branches of the rhododendron aureum in late October, carrying out low-temperature dormancy on the branches, carrying out water planting to promote the germination and the growth of lateral buds and cutting in a form of one segment with one leaf after the treatment to be used as explants for later use, wherein the culture medium for the rooting of the stem segments and the germination and the growth of the lateral buds comprises the components of: the culture medium+ 0.13-0.14mg.L-1 of KT (kinetin)+ 0.05-0.08mg.L-1 of IBA (Indole Butyric Acid)+ 0.02-0.05mg.L-1 of NAA (Naphthyl Acetic Acid)+ 2.70-2.90mg.L-1 of GA(Gibberellin)3. According to the method, the process is simple, the cost is low, the operability ...

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17-04-2013 дата публикации

Rapid after-ripening and germination method of pulsatilla koreana seeds

Номер: CN103039157A
Принадлежит:

The invention relates to a plant propagation technology and provides a rapid after-ripening and germination method of pulsatilla koreana seeds. The rapid after-ripening and germination method comprises the following steps of: immersing the pulsatilla koreana seeds by utilizing a solution mixed by KT with the concentration of 2.10-2.30mg/L, GA3 with the concentration of 2.90-3.20mg/L and 2,4-D with the concentration of 0.30-0.60mg/L; uniformly mixing the seeds with river sand and putting the mixture into a wood groove; placing the wood groove into a room to carry out poikilothermic treatment for 24-28 days, wherein the poikilothermic treatment amplitude and time are as follows: the temperature at daytime is 25+/-2 DEG C and the treatment time is 14 hours; and the temperature at night is 10+/-2 DEG C and the treatment time is 10 hours; and treating for 24-28 days and directly sowing the seeds. The pulsatilla koreana seeds can germinate after being treated by the method and sowed for 10-14 ...

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24-04-2013 дата публикации

Preparation method for bacteria liquid beneficial for growth-development and mycorrhiza formation of rhododendron plants

Номер: CN103060207A
Принадлежит:

The invention relates to a bacteria liquid for growth and development of plants, and in particular relates to a preparation method for bacteria liquid beneficial for growth-development and mycorrhiza formation of rhododendron plants. The method comprises the following steps: (1) preparation and treatment of materials: collecting and disinfecting mature fibrous roots of rhododendron plants, and cutting the treated mature fibrous roots to root segments for later use; (2) culture medium configuration: the content of 1000mL of culture medium comprises the following components of 380mg of KH2PO4, 149mg of MgSO4.7H2O, 26mg of vitamin C, 67mg of calcium pantothenate, 1.4mg of nicotinic acid, 3.0g of peptone, 16.5g of glucose, 8.5g of agar powder, 4mL of 1.5% of sodium deoxycholate liquid and 0.05mg of indoleacetic acid; (3) preparation of mother liquid; and (4) preparation of the bacteria liquid. The preparation method provided by the invention provides a technical method for production, application ...

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17-04-2013 дата публикации

Basal culture medium formula suitable for azalea tissue culture

Номер: CN103039367A
Автор: Gu Dizhou, Gu Chuanyue
Принадлежит:

The invention relates to the field of plant reproduction, particularly a basal culture medium formula suitable for azalea tissue culture. The culture medium comprises the following components: (1) macroelements: 375mg.L<-1> NH4NO3, 180mg.L<-1> KNO3, 320mg.L<-1> CaCl2.2H2O, 245mg.L<-1> MgSO4.7H2O and 436mg.L<-1> KH2PO4; (2) iron salts: 18.4mg.L<-1> FeSO4.7H2O and 24.9mg.L<-1> Na2.EDTA.2H2O; (3) microelements: 12.4mg.L<-1> MnSO4.4H2O, 7.2mg.L<-1> ZnSO4.7H2O, 5.7mg.L<-1> H3BO3 and 0.05mg.L<-1> Na2MO4.2H2O; (4) organic components: 0.3mg.L<-1> aneurin (VB1) and 75mg.L<-1> inositol; and (5) others: 7.8g.L<-1> agar powder with 15.0g.L<-1> sucrose as well as cytokinin variety and bios variety components, wherein the pH value is 5.6. The invention has the characteristics of low component consumption and low price, and is suitable for quick growth of azalea.

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17-04-2013 дата публикации

Test tube one-step seedling method of taxus cuspidata

Номер: CN103039365A
Принадлежит:

The invention relates to a plant planting technology, namely a test tube one-step seedling method of taxus cuspidate. The test tube one-step seedling method comprises the following steps of: (1) taking a newly-sprouted main stem on a taxus cuspidate wild seedling trunk and cutting into segments as explants for further use; (2) when stem segments grow roots, utilizing an axillary bud germination growth culture medium including components and contents: a culture medium+indoleacetic acid IAA (0.03-0.04mg/L)+naphthylacetic acid NAA (0.02-0.03mg/L)+kinetin KT (0.11-0.12mg/L)+gibberellin GA3 (2.60-2.80mg/L); adding agar powder with the concentration of 8.0g/L and adding cane sugar with the concentration of 20.0g/L; adjusting the pH (Potential of Hydrogen) value to 5.6; and culturing tender stem segments under the conditions that the illumination period is 6 hours per day, the illumination intensity is 700lx and the temperature is 20+/-2 DEG C. The invention further provides high-quality seedlings ...

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17-04-2013 дата публикации

Simple fast after-ripening method of acanthopanax sessiliflorus seeds

Номер: CN103039156A
Принадлежит:

The invention relates to a seed propagation technology, and particularly relates to a simple fast after-ripening method of acanthopanax sessiliflorus seeds. The method comprises the steps of soaking the seeds with GA3 and 2,4-D mixed solution and then fishing out; uniformly mixing the seeds and river sand, and then putting the mixture into a wood trough; and performing poikilothermic treatment indoors, wherein in the first stage, the poikilothermic amplitude is 24+/-2 DEG C at daytime and the treatment time is 12 hours, the poikilothermic amplitude is 15+/-2 DEG C at night and the treatment time is 12 hours, and the treatment of the first stage lasts for 80-95 days; and in the second stage, the poikilothermic amplitude is 20+/-2 DEG C at daytime and the treatment time is 8 hours, the poikilothermic amplitude is 5+/-2 DEG C at night and the treatment time is 16 hours, and the treatment of the second stage lasts for 70-75 days. The occurrence rate of cotyledon embryo of embryo can exceed ...

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22-05-2013 дата публикации

In-vitro direct induction and mycorrhization germchit rapid propagation method of rhododendron mycorrhiza

Номер: CN103109746A
Принадлежит:

The invention relates to a plant propagation technology, and particularly relates to an in-vitro direct induction and mycorrhization germchit rapid propagation method of rhododendron mycorrhiza. The method comprises the following steps of: 1, treating an explant material, to be specific, picking a tender shoot newly germinated by rhododendron, which is close to the root part, in the middle ten days of August, disinfecting and cutting into a 1-2-leaf section to be used as the explant for later use; 2, enabling a culture medium induced by rhododendron mycorrhiza to be a basic culture medium and KT0.50mg.L <-1> and IAA0.02mg.L<-1> and GA30.70mg.L<-1> to obtain the explant; 3, culturing the explant on the basic culture medium and ZT2.20mg.L<-1> into a small and tender axillary bud, after the axillary bud grows to 1.00-2.00cm, cutting the axillary bud from the axillae and grafting to the basic culture medium and KT0.50mg.L<-1> and IAA0.02mg.L<-1> and GA30.70mg.L<-1>, culturing for 60d, counting ...

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22-05-2013 дата публикации

Rapid seedling culturing method of pinus sylvestris in tube

Номер: CN103109728A
Принадлежит:

The invention relates to plant propagation technology, in particular to a rapid seedling culturing method of a pinus sylvestris in a tube. The method comprises the following steps: (1), a branch of a trunk of a wild pinus sylvestris plant is cultured in water, the stem section and stem tip are remained for spare use as explants after processed; (2), 0.07 mg/L to 0.08 mg/L -1 of indolebutyric acid, 0.03 mg/L to 0.04 mg/L -1 of naphthylacetic acid, 0.16 mg/L to 0.17 mg/L -1 of zeatin and 2.90 mg/L to 3.20 mg/L -1 of gibberellin are added to a minimal medium; and (3), rapid propagation, seedling hardening and transplanting are conducted to the pinus sylvestris plant. The main stem section of the wild pinus sylvestris plant is taken as the materials, the main stem section is controlled to take root in the tube and meanwhile a main stem bud germinates and grows so that single-step rapid propagation purposes of stem proliferation are achieved, the problems that seeds are difficult to propagate ...

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13-03-2013 дата публикации

Method for effectively and rapidly growing seedlings of smilax riparia

Номер: CN102960098A
Принадлежит:

The invention relates to a plant culture technology, and in particular relates to a method for effectively and rapidly growing seedlings of smilax riparia. The method comprises the following steps of: soaking the seedlings into 3.50-3.75mg.L-1 of 2,4-dichlorphenoxyacetic acid; taking the seedlings out from the acid; uniformly mixing the seedlings with river sand; filling the mixture into a wood groove with good ventilation property; and covering a plastic film in a room to perform poikilothermic treatment of 70-80d, wherein for the poikilothermic range and time, poikilothermic treatment is performed for 10h at the temperature of 22+/-2 DEG C in daytime and is performed for 14h at the temperature of 12+/-2 DEG C at night. According to the method, the seedlings are treated in a one-step manual after-ripening mode so as to fulfill the aims of embryo morphogenesis and physiological after-ripening; and the seedlings subjected to the 2,4-D treatment and the poikilothermic treatment are not required ...

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17-04-2013 дата публикации

Plant source preparation liquid for accelerating crop injurious insect larvae to be rapidly transfigured and preparation method

Номер: CN103039537A
Принадлежит:

The invention relates to a pesticide and provides plant source preparation liquid for accelerating crop injurious insect larvae to be rapidly transfigured and a preparation method. The plant source preparation liquid is formed by mixing extracting liquid of the root of Chinese pulsatilla, aristolochia contorta stems and leaves and polygonum hydropiper stems and leaves. The preparation method comprises the following steps of: respectively weighing the root of Chinese pulsatilla, the aristolochia contorta stems and leaves and the polygonum hydropiper stems and leaves and putting into a mortar to be damped into slurry; immersing by distilled water and carrying out three times of distillation by water steam; and filtering distilled liquid of the three times to obtain the product. A method for spraying ground parts of crops by using a plant source water-soluble component mixed solution to enable the injurious insect larvae to be rapidly pupated or directly cocooned to insects to fly away from ...

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17-04-2013 дата публикации

Integrated after-ripening method of taxus cuspidate seeds

Номер: CN103039155A
Принадлежит:

The invention relates to the field of treatment of plant seeds, namely an integrated after-ripening method of taxus cuspidate seeds. The integrated after-ripening method comprises the following steps that: variable-temperature treatment of seeds is divided into two phases; variable-temperature amplitude and time of the first phase are as follows: the temperature at daytime is 23+/-2 DEG C and the treatment time is 14 hours; the temperature at night is 15+/-2 DEG C and the treatment time is 10 hours; the treatment time of the first phase is 80-95 days; and variable-temperature amplitude and time of the second phase are as follows: the temperature at daytime is 15+/-2 DEG C and the treatment time is 6 hours; the temperature at night is 3+/-2 DEG C and the treatment time is 18 hours; the treatment time of the first phase is 75-90 days. The taxus cuspidate seeds can finally grow to directly-germinated mature cotyledon embryo seeds from a heart-shaped embryo post-period or cotyledon embryo initial ...

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13-03-2013 дата публикации

Rapid after-ripening method of stichopus japonicus seed

Номер: CN102960095A
Принадлежит:

The invention relates to a plant cultivation technology and discloses a rapid after-ripening method of stichopus japonicus seed. The method comprises the following steps of: soaking seeds by using a mixed solution of 2.40-2.60mg.L<-1> gibberellin and 2.50-2.60mg.L<-1> 2,4-dichlorphenoxyacetic acid, taking the seeds out, uniformly mixing the seeds and river sand and filling the mixture into a wood trough, covering the wood trough with a plastic film, placing the wood trough in a room, and carrying out alternating temperature treatment for 105-119 days, wherein the alternating temperature treatment is carried out for 10 hours at the temperature of 22+/-2 DEG C at daytime and carried out for 10 hours at the temperature of 8+/-2 DEG C at night. The problems that the seeds do not sprout in the seeding year and the rooting percentage of vegetative propagation, such as cottage, is extremely low are solved, and the method has the characteristics of short after-ripening time, high operability and ...

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17-04-2013 дата публикации

Fast growing method of rhododendron plants on Changbai mountains

Номер: CN103039258A
Принадлежит:

The invention relates to a plant growing method, namely in particular to a fast growing method of rhododendron plants on changbai mountains. The fast growing method includes the following steps: (1) selecting rhododendron plant seedlings on Changbai mountains with height as 3.0-3.5cm and trunk diameter as 1.0mm; and (2) planting the seedlings in a feeding block of a mixed base material including 4 parts of turfy soil, 2 parts of decomposed pine needle, 2 parts of broad leaf, 1 part of vermiculite, 1 part of cow dung and 0.5 part of compound fertilizer, placing the seedlings into a small shed, tightly sealing the small shed through a plastic film, controlling illumination hour to be 9-15 h.d-1, illumination intensity to be 600-1300/x and temperature to be 25-30 DEG C and applying nutrient solution every 7-22 days. The average growth rate of the rhododendron plant seedlings on Changbai mountains can reach over 142.4% after 45 days, and the seedling trunk diameter reaches 1.89-3.20mm in average ...

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13-03-2013 дата публикации

In-vitro efficient seedling cultivation method synchronous in rooting and growing by utilizing tender stem segments of thuja koraiensis

Номер: CN102960249A
Принадлежит:

The invention relates to plant propagation and in particular relates to an in-vitro efficient seedling cultivation method synchronous in rooting and growing by utilizing tender stem segments of thuja koraiensis. A culture medium for the simultaneous growing of roots and the stem segments comprises the components: the culture medium+ 0.06-0.08mg.L-1 of ZT (Zeatin)+ 0.01-0.03mg.L-1 of NAA (Naphthyl Acetic Acid)+ 0.02-0.04mg.L-1 of IAA (Indole Acetic Acid); in the culture medium, 8.0g.L-1 of agar and 15g.L-1 of cane sugar are added; and the method comprises the steps of: adjusting the pH (potential of Hydrogen) value of the culture medium to be at 5.6, culturing the tender stem segments under the conditions that the photoperiod is 10h.d-1, the illumination intensity is 900lx and the temperature is 23-27 DEG C, planting small plants after the expanding propagation of the seedlings in a matrix comprising the components of disinfected peat, rotten pine needle and river sand in the ratio of 4: ...

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22-05-2013 дата публикации

Efficient industrialized breeding method for northeast loach

Номер: CN103109768A
Принадлежит:

The invention relates to fish breeding technology, in particular to an efficient industrialized breeding method for northeast loaches. The method comprises the following steps: (1) male loaches and female loaches living for 2 to 3 years are chosen and are respectively fed for 40 to 50 days; (2) each female loach receives subcutaneous injection of parturition hastening medicine liquid at 0.0500to 0.0575 mL/g; (3) after the subcutaneous injection, the male loaches and the female loaches are respectively fed continuously for 12 to 14 days; (4) the male loaches and the female loaches are fed in a mixed mode, after copulation and egg-laying are completed, the female loaches and the male loaches are taken away, and after eggs are hatched, an average hatchability is over 99.8%; and (5) loach fries are fed. The method solves the problems that fry rate is low in natural breeding, copulation is incomplete, hatchability for a germ cell is low, the fries are threatened by small natural enemies so that ...

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17-04-2013 дата публикации

Industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants

Номер: CN103039366A
Принадлежит:

The invention relates to the field of plant propagation, and particularly provides an industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants, which comprises the following steps: (1) treating branches of Changbai Mountain rhododendron plants, and taking the treated branches as explants for later use; (2) preparing a culture medium for enabling stems to take root and axillary buds to germinate and grow simultaneously: adding kinetin KT (0.03-0.010 mg.L<-1>), indolebutyric acid IBA (0.10-0.45 mg.L<-1>), naphthylacetic acid NAA (0.06-0.13 mg.L<-1>), gibberellin GA3 (1.40-2.60 mg.L<-1>), agar powder (7.8 g.L<-1>) and sucrose (10.0 g.L<-1>) into a basic culture medium, and regulating the pH value to 5.6; and culturing under the conditions that the illumination period is 9 h.d<-1>, the illumination intensity is 1000 lx and the temperature is 23+/-2 DEG C; (3) performing mycorrhizal treatment on tissue culture seedlings; and (4) culturing the tissue culture ...

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20-03-2013 дата публикации

One-step after-ripening method of magnolia sieboldii seed

Номер: CN102972122A
Принадлежит:

The invention relates to a one-step after-ripening method of a magnolia sieboldii seed. The method comprises the steps of soaking the seed with mixed solution of 1.10-1.30mg.L-1 of 6-benayl aminopurine and 2.10-2.30mg.L-1 of 2,4-dichlorphenoxyacetic acid, then fishing the seed out, evenly stirring the seed with river sand and then packaging into a wood tank with good air permeability; and covering a plastic film and arranging the wood tank inside a room to carry out 93-114d of poikilothermic treatment, wherein the temperature range and time are that the temperature during the day is 18+/-2 DEG C, the treatment time is 16 hours, the temperature at night is 7+/-2 DEG C, and the treatment time is 8 hours, and then the seed develops to become a mature embryo cotyledon seed. According to the method, not only are the difficulties that the seeds do not germinate or a few of seeds germinate in the same year of being sown and the rooting percentage of vegetative propagation such as cottage is very ...

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22-05-2013 дата публикации

Integrated detoxification method of vitis vinifera in test tube

Номер: CN103109744A
Принадлежит:

The invention relates to an integrated detoxification method of vitis vinifera in a test tube, belonging to the technique of plant propagation. The method comprises the following steps: (1) conducting water cultivating on dormant branches of the vitis vinifera to impel dormant buds to sprout, employing treated stem tips as explants for standby; (2) inoculating the cut vitis vinifera stem tips to a minimal medium added with 0.08-0.13mg.L of indolebutyric acid, 2.20-3.30mg.L of gibberellins, 5g.L of edible sugar and 8.0g.L of agar stripes for cultivating to remove a grapevine fanleaf virus and a grapevine leafroll associated virus; (3) controlling the cultivating temperature to be 45-48 DEG C, and cultivating for 51-57 days; and (4) when the stem tip grows to 1.0cm, cutting 0.03-0.05cm of stem tip to further carry on nontoxic seedling cultivation. Through the application of the integrated detoxification method, the removal rates of the grapevine fanleaf virus and the grapevine leafroll associated ...

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