微生物同步发酵正十四烷生产十二烷1,12-二羧酸的方法

... 本发明公开了一种利用微生物同步发酵正十四烷(nC#-[14])高产十二烷1，1 2-二羧酸(DC#-[14])的方法。所用微生物为一株热带假丝酵母(Candida Tropicalis)优良生产突变株NP-6-5。其特点是：在微生物菌种接入含有C#-[11]-C#-[18]各种正烷烃为基质的培养基后，28小时内，pH控制在6.8以下以菌体生长为主，产生一定数量的二元酸；28-60小时，pH控制在7.5以下，以产酸为主，也增长一定数量菌体；60小时以后，pH控制在7.5-8.5，迅速产生各种二元酸。当本方法用于nC#-[14]发酵生产DC#-[14]时，在10L自控罐内，发酵6天，DC#-[14]产量高达201g/L，DC#-[14]的纯度达到97-98％。 ...

Method for producing undecane-1,11-bicarboxylic acid by microorgan fermenting synchronously

A synchronous microbe fermentation process for producing hendecane 1,11-dicarboxylic acid (DC13) with high output from n-tridecane (nC13) features that after the mutational strain resultant from Candidatropicalis is inoculated to culture medium whose matrix is different normal alkanes containing C11-C18, its main action is to grow thallus within 28 hrs, generating a certain quantity of biatomic acid by controlling pH value under 6.8, then to generate acid while growing a certain amount of thallus in 28-60 hrs by controlling pH value under 7.3, and finally to quickly generate different biatomic acids after 60 hrs by controlling pH value to 7.5-7.8. When the process is used to produce DC13 by fermentation in 2.5 cu.m fermentator, the DC13 content is high up to 205 g/l in 161 hrs, the transform rate is 94% and the purity of DC13 is 96-97%.

Parallel arc-shaped railing type restraint frame

The invention relates to a parallel arc-shaped railing type restraint frame. The parallel arc-shaped railing type restraint frame consists of two groups, wherein each group consists of parallel arc-shaped railings, cross rods, push-and-pull rods, rollers and blocking plates, one end of each parallel arc-shaped railing is connected with a corresponding cross rod through shafts and is connected with the corresponding cross rod through a corresponding supporting rod, of which the two ends are provided with hookers, the push-and-pull rods and the cross rods are connected through sticking, the rollers are connected to the lower ends of the parallel arc-shaped railings, which are connected with the two ends of each cross rod, through shafts, and the blocking plates are respectively plugged in a plug slot in one side of each parallel arc-shaped railing. The parallel arc-shaped railing type restraint frame has the advantages that the structure is simple, the operation is convenient, the number ...

Electromechanical type retaining frame

The invention discloses an electromechanical type retaining frame, which consists of two groups, wherein each group respectively consists of parallel arc-shaped handrails, transverse rods, push and pull rods, rollers and shielding plates, one end of each parallel arc-shaped handrail is connected with the corresponding transverse rod through a shaft, and is connected with the corresponding transverse rod through a support rod of which the two ends are provided with hooks, each push and pull rod is welded with the corresponding transverse rod, the rollers are connected with the two ends of the corresponding transverse rod and the lower end of the corresponding parallel arc-shaped handrail through bearings, and each shielding plate is hung at one side of the corresponding parallel arc-shaped handrail. The electromechanical type retaining frame has the advantages that the structure is simple, the operation is convenient, the primary retaining number is more, the effect is good, the epidemic ...

Microbial synchronous n-tetradecane fermenting process to produce tetradecadicarboxylic acid

The present invention discloses a microbial synchronous n-tetradecane (nC14) fermenting process to produce tetradecadicarboxylic acid (DC14). The microbe used is one high-yield Candida Tropicalis mutant strain H-12-112. The present invention features that within 48 hr after microbe seed is inoculated into culture medium with C11-C18 n-alkane as matrix, the pH is controlled below 7.5 for thallus to grow to produce certain amount of binary acids; from 48 to 120 hr, the pH is controlled below 8.0; and afte 120 hr the pH is controlled below 8.5 for producing various binary acids. When the said process is used in nC14 fermentation to produce DC14 in a 25-ton tank, after fermentation for over 6 days, can reach DC14 yield up to 242.8 g/L, post-treatment yield 82% and DC14 purity up to 98.2%.

Process for producing long-chain alpha omega dicarboxylic acid by synchronous fermentation of microbe

Papaya steak and method for preparing same

The invention belongs to the field of foods, in particular to papaya steak and a method for preparing the same. The papaya steak is cooked by using the following raw materials in part by weight: 100 parts of steak, 10 to 20 parts of smashed papaya, 1 to 2 parts of brandy, 0.5 to 1.0 part of vanilla, 2.2 to 2.8 parts of seasoning and 0.035 part of food additive. The papaya steak of the invention is rich in nutrition and convenient for industrialized production, and can be easily cooked by consumers.

Method for producing undecane-1,11-bicarboxylic acid by microorgan-fermenting n-trideane synchronously

Method for Microbial synchronous fermentation of n-tetradecane to produce tetradecadicarboxylic acid

Process for producing long-chain alpha, omega-dicarboxylic acid by synchronous fermentation of microbe

A microbe synchronous fermentation process for producing long-chain alpha, omaga-dicarboxylic acid, especially dodecadiatomic acid (DC12) with high output features that after microbe strain (excellent saltant of Candida tropicalis) is inoculated into culture medium whose matrix is n-alkanes containing C11-C18, pH is controlled to 6.0-6.8 for thallus growth as priority while also production of diatomic acids by limited output, and when optical density of thallus growth (OD(X30,620nm)) reaches 0.6 and pH is controlled to 7.0-7.8, different diatomic acids with the same chain length as matrix is greatly produced. After inoculation for 40 hr, when acid output reaches 33.3 g/L, then output of acid is transferred to for fermentation as priority and can reach 145 g/l in 130 hr after inoculation.

Answering method and device and electronic equipment

The invention provides an answering method and device and electronic equipment, and the method comprises the steps: responding to a selection operation for a target question, displaying a plurality of guiding questions used for answering the target question, and enabling the answering sequence of the guiding questions to be the same as the solving sequence indicated by the solving strategy of the target question; responding to the answering operation for each guiding question, and displaying the answering result of the corresponding guiding question. According to the method provided by the invention, a complete question solving logic chain belonging to the target question can be established through a plurality of guiding questions, the question raising mode and thought during explanation of a teacher are simulated, the user is guided to solve the target question, the ability of the user to autonomously solve the problem is improved, and thus the learning effect of the user is improved.

Process for preparing dodecyl-1,12-bicarboxylic acid from n-tetradecane by microbial synchronous fermentation

A process for preparing dodecyl-1,12-dicarboxylic acid (DC14) by microbinal synchronus fermentatino of n-tetradecane (nC14) features that said microbe is a mutant NP-6-5 of Candida tropicalis, and includes inoculating the microbe in the culture medium containing C11-C18 n-alkanes and fermenting in autocontrol tank for 6 days. Its advantages are high output (201 g/L) and high purity up to 97-98%.

New method for biosynthetic production of mixed long-chain dibasic acid

The invention discloses a preparation method for long chain Alpha, Omega-dicarboxylic acid, in particular the high yield C11- C14 mixed long chain dibasic acid (DCm) by use of biosynthesis. The microorganism adopted is CH-14-328 mutant strain, which belongs to Candida tropicalis. The preparation method is characterized that: the microorganism is introduced into the culture medium with the substrates of various normal alkanes of C11 - C18. Then, at the first stage, the system is controlled to mainly focus on the cell growth, and the normal alkanes are also transformed to produce dibasic acid; at the second stage, the reaction system is controlled to concentrated on producing acid, as well as growing a part of cells; at the third stage, the system is confined to produce acid only, without growing any cells. The long chain Alpha, Omega-dicarboxylic acid can be applied to transform No.200 light wax oil for producing DC11 - DC14 mixed dibasic acid; during the test in 5m<3> fermenter, up to 165g ...

Method for producing saturated and unsaturated alpha, omega-dicarboxylic acid with microbial transformation of oil and fat

The invention discloses a method of utilizing microbial transformation grease to produce saturated and unsaturated Alpha- or Omega- dicarboxylic acid, in particular to a method of producing 9-Octadecadienoic acid (Delta<9>DC18). The microorganism to be used is ou-3 mutant strain and belongs to Candida tropicalis. The invention is characterized in that after the microorganism strain is inoculated into the culture medium of containing a substrate of oleic alcohol, oleic acid and methyl oleate, cell growth of the system is mainly controlled and grease is also transformed to produce binary acid at the first stage; the reaction system is mainly controlled on acid production and also on growth of some cell at the second stage; the system is only controlled on acid production and without the cell growth. The method is used to transform the oleic alcohol to produce 9-Octadecadienoic acid (Delta<9>DC18), the output of Delta <9>DC18 reaches 110.5 grams per Liter in 16 liter fermentation tank for ...

Method for producing saturated and unsaturated alpha, omega-dicarboxylic acid with microbial transformation of oil and fat

The invention discloses a method of utilizing microbial transformation grease to produce saturated and unsaturated Alpha- or Omega- dicarboxylic acid, in particular to a method of producing 9-Octadecadienoic acid (Delta<9>DC18). The microorganism to be used is ou-3 mutant strain and belongs to Candida tropicalis. The invention is characterized in that after the microorganism strain is inoculated into the culture medium of containing a substrate of oleic alcohol, oleic acid and methyl oleate, cell growth of the system is mainly controlled and grease is also transformed to produce binary acid at the first stage; the reaction system is mainly controlled on acid production and also on growth of some cell at the second stage; the system is only controlled on acid production and without the cell growth. The method is used to transform the oleic alcohol to produce 9-Octadecadienoic acid (Delta<9>DC18), the output of Delta <9>DC18 reaches 110.5 grams per Liter in 16 liter fermentation tank for ...

Novel high efficiency beef cutting machine

The utility model discloses a novel high efficiency beef cutting machine, include the cutting bed and connect the support column in its four corners, be connected with the diaphragm between four the support column, install double -shaft motor on the diaphragm, double -shaft motor's first drive shaft is connected with first sprocket through first transfer line, and first sprocket is connected withtwo second sprockets through a chain drive, and first sprocket is triangular distribution with two second sprockets, and second sprocket coaxial coupling has a spiral shell rasp bar, and spiral shellrasp bar rotates and connects on the cutting bed top surface, and two spiral shell rasp bar are close to the cutting bed respectively and set up at both ends about, and spiral shell rasp bar goes up the cooperation and is connected with screw sleeve, just is connected with the push pedal between two screw sleeve. The utility model discloses but not only improve the efficiency of beef cutting, still reduced ...