Method for constructing food-borne hyperoxaluric calcium oxalate calculus model

The invention relates to a method for constructing a food-borne hyperoxaluric calcium oxalate calculus model, which belongs to the technical field of animal moulding. The method is characterized in that male beagle at 1.5-2.0 years old with 8-10Kg weight, a canine penton vaccine is used for immunization, basic dog food and oxalic acid are used after 7 days for moulding, the addition of oxalic acid during a moulding period accounts for 0.8-1.5wt% of the basic dog food, and the moulding time is 25-30 days. The employed food-borne moulding method can increase the security and reproducibility for moulding, the moulding success rate is high, and effect is good.

Cow heat-stress resistant feed additive

The invention discloses a cow heat-stress resistant feed additive. Saccharomyces cerevisiae (Sc) NAU-ZH-GY1 is applicable to prepare the cow heat-stress-resistant feed additive. The cow heat-stress-resistant feed additive, which is rich in glycerin, yeast live bacteria and microbial metabolites such as amino acids, vitamins and the like, is produced under the high-permeability condition by employing a continuously feeding manner for fermentation; and the cow heat-stress resistant feed additive is capable of cooperatively perform dual efficacy of glycerin and yeast, and is capable of substantially improving lactation yield, 4% fat corrected milk yield and milk fat percentage of cow under heat-stress condition, improving blood sugar concentration, reducing somatic cell number, concentration of non-esterified fatty acid and concentration of urea nitrogen in milk, supplementing energy for cows, alleviating body weight reduction, enhancing antioxidation capability, reducing HSP70mRNA level and ...

Dog urinary stone chemical component analysis method and chemical component analysis equipment

The invention relates to the field of dog urinary stone detection analysis and discloses a dog urinary stone chemical component analysis method and chemical component analysis equipment. A dog urinary stone sample is acquired, cleaned, dried and ground; then the dog urinary stone sample is equally divided into seven parts which are contained in different reaction test tubes; different reaction test tubes are added with different chemical reaction reagents in a dropping manner, and oscillating mixing is carried out; and the chemical composition of the dog urinary stone sample can be judged according to the reaction phenomenon in the reaction test tube after a preset time period. According to the dog urinary stone chemical component analysis method and the chemical component analysis equipment, qualitative chemical analysis automation is realized, the operation is simple and rapid, special complex instrument is not required, the method and the equipment have important significance to clinical ...

Silage rich in organic selenium

The invention relates to a method for preparing silage rich in organic selenium and belongs to the technical field of silage production. Streptococcus thermophilus, beer yeast and sodium selenite into silage are added in silage raw materials; and in the process of fermenting the silage, the sodium selenite is converted into selenomethionine by microbes added into the silage and original microbes to finally obtain the silage rich in the organic selenium (the content of the organic selenium is improved to over 0.02 mg.Kg<-1> from original 0.01), and the quality of the silage is improved simultaneously (the content of crude protein is improved and the content of ammoniacal nitrogen is reduced). The silage can achieve the aim of replenishing the organic selenium while providing high-quality silage for herbivores, thereby improving the immunity and anti-oxidation capacity of animals, strengthening the resistance of the animals to diseases, improving the production performance of the animals, ...

Oxalate-degrading strain NJODE1 and application thereof

The invention discloses an oxalate-degrading strain NJODE1 and application thereof. The oxalate-degrading strain NJODE1 has a classification name of Enterococcus faecium with the preservation number of CGMCC NO. 4581. The oxalate-degrading strain NJODE1 has good tolerance to acids and bile salts, has no antibiotic resistance to most non-antibiotics, is safe for mice and can be colonized in dogs. The NJODE1 can reduce gastrointestinal absorption of oxalate in foods, thereby reducing the content of oxalate in urine, and thus reducing the incidence of canine calcium oxalate calculus. Whether thefinished product of the strain is food or pharmaceutical products or yogurt and fermented milk, the strain has good resistance to acids and bile salts, contains safe enterococci, and has significant effects on lowering the urinary oxalate content and preventing and treating calcium oxalate calculus of pets, thus having broad application prospects.

Glycerol-producing saccharomyces cerevisiae NAU-ZH-GY1 and application thereof

The invention provides glycerol-producing saccharomyces cerevisiae NAU-ZH-GY1 and application thereof, belonging to the field of microbial fermentation. The fungus is separated from unprocessed honey and is identified as saccharomyces cerevisiae with a collection number of CGMCC (China General Microbiological Culture Collection Center) No. 4551. The strain is obtained through liquid fermentation; fermentative culture comprises abundant glycerol, a large amount of living saccharomyces and rich microbial metabolism substances such as amino acid, vitamins and the like; the saccharomyces cerevisiae is applied to producing a feedstuff additive at the perinatal period of a ruminant; the glycerol can be used as an energy supplement to relieve negative energy balance; by means of the saccharomyces cerevisiae and the microbial metabolism substances such as amino acid, vitamins and the like, animal gastrointestinal tract flora can be adjusted, pathogenic microorganisms can be antagonized, immunity ...

Microbiological feed additive for adjusting energy metabolism impairment of flocks and herds during perinatal period

The invention relates to microbiological feed additive for adjusting energy metabolism impairment of flocks and herds during perinatal period, which belongs to the production field of feed additives. A preparation method of the microbiological feed additive comprises the following steps: adding inorganic selenium source into a microbial inoculum culture medium to ensure that the concentration of selenium in the culture medium is 2 to 10 mg/kg; inoculating Candida krusei, Candida valida and Streptococcus thermophilus seed liquor in the culture medium according to the ratio of 13:1:2; mixing the culture solution with the a proper amount of corn flour, and drying at 35 to 40 DEG C to obtain the feed additive. The invention adopts the principle that probiotics can convert sugar into glycerin and convert inorganic selenium into organic selenium to produce the complex probiotic product which is rich in glycerin and organic selenium, can exert the efficacies of glycerin, organic selenium andprobiotics ...

Composition for establishing chronic glomerulonephropathy model and application thereof

The invention relates to a composition for establishing a chronic glomerular nephropathy model, an establishing method of the chronic glomerular nephropathy model and application of the chronic glomerular nephropathy model in treatment of chronic glomerular nephropathy. In the modeling method, ochratoxin A with a low dose and no toxicity and adriamycin with a low dose are used. According to the application, adriamycin is given to BAL/bc mice in a single caudal vein mode, ochratoxin A is injected to the intraperitoneal cavity every day to jointly act for 14 days, a mouse chronic glomerular nephropathy model is obtained, and the chronic glomerular nephropathy model is verified through in-vitro human glomerular mesangial cells; the mouse model is similar to a traditional model in the change process of chronic glomerulonephropathy formed by single administration of high-dose adriamycin, and the dosage of adriamycin is reduced by 4 times due to the use of ochratoxin A, so that the side effect ...

Recombination porcine parvovirus VP2 virus particles for expressing exogenous gene

The invention relates to construction and application of recombinant porcine parvovirus (PPV) VP2 nucleocapsids which express foreign genes, belonging to the genetic engineering bacterin field. C-terminal gene fragments of PPV VP2 genes are cloned into a type 5 adenovirus shuttle vector of the human beings, and recombinant adenovirus rAd-deltaVP2 is obtained; deltaVP2 proteins are expressed successfully and highly efficiently and can be self-assembled into the nucleocapsids [PPV:VLPs]; the PPV VP2 nucleocapsids are used as antigen transport vectors and the porcine circovirus type 2 nucleocapsid protein (Cap) genes are embedded into an N-terminal (deltaVP2) of the PPV VP2 gene, and then recombinant adenovirus rAd-deltaCap-deltaVP2 is obtained; embedded VP2 (deltaCap-deltaVP2) proteins are expressed successfully and highly efficiently and can be self-assembled into nucleocapsids [PPV:VLP(PCV2)]. The invention also relates to application of the recombinant virus and recombinant PPV VP2 nucleocapsids ...

Method for producing selenium-riched low-cholesterol eggs and the products thereof

The invention relates to a method for preparing selenium-rich low-cholesterol egg and the relevant product, belonging to health-care functional egg product production field, which comprises following steps: employing lactobacillus acidophilus and candida as bacteria, the culture medium is 0.5-2% yeast concrete and 0.5-2% glucose, inoculating bacteria in sodium selenite containing culture medium, culturing for 30-40hours, getting culturing solution containing selenium-rich probiotics, adding culturing solution in basic corn bean meal type ration , feeding chicken with it, and getting selenium-rich low-cholesterol egg products. The selenium content in egg products is 0.358-0.508 mg/ kg (wet weight), cholesterol in egg yellow content is 11.37-12.17 mg/ g (wet weight). The egg product is characterized by abundant nutrition, selenium supplement, low-cholesterol content, health-care function and effect of prevention of cardiovascular disease.

Microbiological feed additive for adjusting energy metabolism impairment of flocks and herds during perinatal period

The invention relates to microbiological feed additive for adjusting energy metabolism impairment of flocks and herds during perinatal period, which belongs to the production field of feed additives.A preparation method of the microbiological feed additive comprises the following steps: adding inorganic selenium source into a microbial inoculum culture medium to ensure that the concentration of selenium in the culture medium is 2 to 10 mg/kg; inoculating Candida krusei, Candida valida and Streptococcus thermophilus seed liquor in the culture medium according to the ratio of 13:1:2; mixing theculture solution with the a proper amount of corn flour, and drying at 35 to 40 DEG C to obtain the feed additive. The invention adopts the principle that probiotics can convert sugar into glycerin and convert inorganic selenium into organic selenium to produce the complex probiotic product which is rich in glycerin and organic selenium, can exert the efficacies of glycerin, organic selenium andprobiotics ...

Composite probiotics preparations with rich organic selenium and organic zincium

The invention relates to a probiotic-preparation rich in organic selenium and organic zinc, and belongs to the food-engineering and additive field. Candida utilis, lactobacillus acidophilus, Lactobacillus rhamnosus and streptococcus thermophilus are used as strains, the mixing proportion of which is 1:2:2:2; the strains are inoculated in an optimum medium which is added with sodium selenite, zinc sulfate, organic selenium-zinc transformation, and growth promoter of probiotics, and then are cultured and freeze-dried under vacuum condition, so that a complex- probiotic-product rich in the organic selenium and the organic zinc is obtained. The amount of CFU can reach more than 10<8>/g, 10<9>/g, 10<8>/g, and 10<8>/g in the product, the content of the organic selenium ranges from 50 to 150 microgrammes/g, and the content of the organic zinc ranges from 2.5 to 7.5mg/g. The probiotic-preparation has three efficacies of the organic selenium, the organic zinc and the probiotics, regulates the intestinal ...

Oxalic acid degrading bacterium NJODL1 and application thereof

The invention discloses an oxalic acid degrading bacterium NJODL1, which is classified and named as Lc.lactis subsp.lactis with the collection number CGMCC No.4582. A strain has the effects of degrading oxalic acid, regulating oxalate metabolism and preventing and controlling calcium oxalate calculus of pets. The oxalic acid degrading bacterium NJODL1 has high tolerance on acids and bile salts, does not have antibiotic resistance on most antibiotics, is safe for mice and can be colonized in dogs. By adopting NJODL1, the absorption of oxalic acid in foods by a gastrointestinal tract can be reduced, the content of oxalic acid in urea is lowered, and the incidence rate of calcium oxalate calculus of dogs is further lowered. Regardless of being prepared into a food or a medicament or yoghourtor acidified milk, the strain has high acid resistance, high bile salt resistance, the effects of remarkably lowering urea oxalic acid and preventing and controlling calcium oxalate calculus of pets,and ...

Recombination virus particles for expressing 2-typed porcine circovirus nucleocapsid protein Cap gene

The invention relates to construction and application of recombinant nucleocapsids of Cap genes of porcine circovirus expression type 2 nucleocapsid protein, belonging to the genetic engineering bacterin field. C-terminal gene fragments of porcine parvovirus (PPV) VP2 genes are cloned into a type 5 adenovirus shuttle vector of the human beings, and recombinant adenovirus rAd-deltaVP2 is obtained; deltaVP2 proteins are expressed successfully and highly efficiently and can be self-assembled into the nucleocapsids [PPV:VLPs]; the PPV VP2 nucleocapsids are used as antigen transport vectors and 165 to 200 sites of amino acid (deltaCap) genes of the porcine circovirus type 2 (PCV2) nucleocapsid proteins (Cap) are embedded into an N-terminal (deltaVP2) of the PPV VP2, and then recombinant adenovirus rAd-deltaCap-deltaVP2 is obtained; embedded VP2 (deltaCap-deltaVP2) proteins are expressed successfully and highly efficiently and can be self-assembled into nucleocapsids [PPV:VLP(PCV2)]. The invention ...

Method for producing selenium-riched low-cholesterol eggs and the products thereof

The invention relates to a method for preparing selenium-rich low-cholesterol egg and the relevant product, belonging to health-care functional egg product production field, which comprises following steps: employing lactobacillus acidophilus and candida as bacteria, the culture medium is 0.5-2% yeast concrete and 0.5-2% glucose, inoculating bacteria in sodium selenite containing culture medium, culturing for 30-40hours, getting culturing solution containing selenium-rich probiotics, adding culturing solution in basic corn bean meal type ration , feeding chicken with it, and getting selenium-rich low-cholesterol egg products. The selenium content in egg products is 0.358-0.508 mg/ kg (wet weight), cholesterol in egg yellow content is 11.37-12.17 mg/ g (wet weight). The egg product is characterized by abundant nutrition, selenium supplement, low-cholesterol content, health-care function and effect of prevention of cardiovascular disease.

Method for producing probiotic yoghurt enriched with selenium amino acid and its products