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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 6. Отображено 6.
19-02-2014 дата публикации

Recombinant HVT (herpesvirus of turkey) expressing IBDV (infectious bursal disease virus) VP2 and bursin chimeric protein

Номер: CN103589693A
Автор: PAN QUNXING, WANG YONGSHAN, OUYANG WEI, WANG XIAOLI, BI ZHENWEI, XIA XINGXIA, ZHU YUMEI, DONG CHENHONG, HE KONGWANG, XIAO QI
Принадлежит:

The invention relates to a recombinant HVT (herpesvirus of turkey) expressing IBDV (infectious bursal disease virus) VP2 and bursin chimeric protein, and belongs to the research field of genetic engineering and biological products. 10 copies of BS 10 (bursin 10) chimeric IBDV VP2 gene expression cassettes are combined with HVT, so that a brand-new recombinant HVT (rHVT-BS10-VP2) is obtained. The virus simultaneously is recombining bivalent vaccine of an MD (Marek's disease) and an infectious bursal disease. The recombining bivalent vaccine not only can have an immune protection effect on the MD of chicken, but also can induce anti-IBDV lasting protective immunity of a commercialized chicken crowd with a high IBDV maternal antibody level. Compared with conventional IBDV and HVT vector live vaccine (vHVT-013-69 strains), the protection rate (100%) of an rHVT-BS10-VP2 immunity class is higher than the protection rate (93.3%) of an rHVT-VP2 immunity class.

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Номер записи: 1
22-10-2008 дата публикации

Recombination porcine parvovirus VP2 virus particles for expressing exogenous gene

Номер: CN0101289657A
Автор: HE KONGWANG, PAN QUNXING, WEN LIBIN, ZHANG XUEHAN, NI YANXIU, YU ZHENGYU, GUO RONGLI, LU LIXIN, MAO AIHUA, XIAO QI, HUANG KEHE, KONGWANG HE, QUNXING PAN, LIBIN WEN, XUEHAN ZHANG, YANXIU NI, ZHENGYU YU, RONGLI GUO, LIXIN LU, AIHUA MAO, QI XIAO, KEHE HUANG
Принадлежит:

The invention relates to construction and application of recombinant porcine parvovirus (PPV) VP2 nucleocapsids which express foreign genes, belonging to the genetic engineering bacterin field. C-terminal gene fragments of PPV VP2 genes are cloned into a type 5 adenovirus shuttle vector of the human beings, and recombinant adenovirus rAd-deltaVP2 is obtained; deltaVP2 proteins are expressed successfully and highly efficiently and can be self-assembled into the nucleocapsids [PPV:VLPs]; the PPV VP2 nucleocapsids are used as antigen transport vectors and the porcine circovirus type 2 nucleocapsid protein (Cap) genes are embedded into an N-terminal (deltaVP2) of the PPV VP2 gene, and then recombinant adenovirus rAd-deltaCap-deltaVP2 is obtained; embedded VP2 (deltaCap-deltaVP2) proteins are expressed successfully and highly efficiently and can be self-assembled into nucleocapsids [PPV:VLP(PCV2)]. The invention also relates to application of the recombinant virus and recombinant PPV VP2 nucleocapsids ...

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Номер записи: 2
07-05-2014 дата публикации

High expression gga-miR-9* chicken embryo fibroblast passage cell strain

Номер: CN103773735A
Автор: OUYANG WEI, WANG YONGSHAN, PAN QUNXING, WANG XIAOLI, XIA XINGXIA, LIU HUAJIE, ZHANG HAIBIN
Принадлежит:

The invention relates to a high expression gga-miR-9* chicken embryo fibroblast passage cell strain DF-miR-9*, and belongs to the field of biotechnology. The gga-miR-9* with the cell endogenous property is applied to an expression system of slow virus to establish a recombinant lentiviral vector VL+miR-9* containing pri-gga-miR-9*, and VL+miR-9* infects chicken embryo fibroblast passage cell line DF-1 to obtain high expression gga-miR-9* chicken embryo fibroblast passage cell strain DF-miR-9*. The DF-miR-9* is used for culturing infectious bursal disease virus (IBDV), and the titer of the IBDV is over 10 times of that of the virus cultured by the common DF-1 cell. The high expression gga-miR-9* chicken embryo fibroblast passage cell strain DF-miR-9* provided by the invention is applied to IBDV antigen preparation and vaccine production, is high in virus titer, and has a large production and application value.

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Номер записи: 3
22-07-2009 дата публикации

Molecular design for simulating IBDV multi-epitope gene and use thereof

Номер: CN0101487011A
Автор: WANG YONGSHAN, WANG ZHONGCAN, TANG YUDE, TAN WEIGUO, PAN YING, SHI ZHENGLIANG, OUYANG WEI, LI YIN, WANG XIAOLI, PAN QUNXING, XIA XINGXIA, ZHU YUMEI, ZHANG GAIPING, WANG XUANNIAN, CHEN GUANGHUA, YONGSHAN WANG, ZHONGCAN WANG, YUDE TANG, WEIGUO TAN, YING PAN, ZHENGLIANG SHI, WEI OUYANG, YIN LI, XIAOLI WANG, QUNXING PAN, XINGXIA XIA, YUMEI ZHU, GAIPING ZHANG, XUANNIAN WANG, GUANGHUA CHEN
Принадлежит:

The invention relates to a molecular design of a simulated IBDV multi-epitope gene and application of the molecular design, and belongs to the field of biopharmaceutical industry. The molecular design synthesizes the simulated IBDV multi-epitope gene epis with immune protection function according to an effective antigen epitope motif design of immune protection, constructs a recombinant expression plasmid pET-epis with genetic engineering techniques, leads the epis gene to be expressed in colon bacillus, and finally prepares a genetically engineered IBDV epitope vaccine with an antigen of a recombinant IBDV multi-epitope protein rEPIS that is expressed in colon bacillus and corresponding adjuvant agents, and the genetically engineered IBDV epitope vaccine can be used for preventing and controlling infectious bursal disease. In the epis molecular design, a linear sequence is adopted to simulate IBDV space conformation dependent epitope motif, and consequently the recombinant rEPIS that is ...

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Номер записи: 4
02-09-2015 дата публикации

Efficient-secretion canine parvovirus resistant monoclonal antibody hybridoma cell A135 strain

Номер: CN104877968A
Автор: WANG YONGSHAN, XIA XINGXIA, BI ZHENWEI, LI YUEHUA, ZHU YUMEI, OUYANG WEI, PAN QUNXING, WANG XIAOLI, DONG CHENHONG, MEI YONGJIE, WANG JINGYU, WU HONGLING
Принадлежит:

The invention relates to an efficient-secretion canine parvovirus (CPV) resistant monoclonal antibody hybridoma cell A135 strain and belongs to the technical field of biology. The hybridoma cell A135 strain selected from the secretion CPV resistant monoclonal antibody hybridoma cell bank is excellent in biological performance and is injected with ascetic fluid generated in a BALB/C mouse peritoneal, so that neutralizing titer is up to 1010. In addition, the hybridoma cell A135 strain has moderate neutralizing capacity for various CPV subtype virus strain such as CPV-2a, CPV-2b, CPV-2c (a) and CPV-2c (b) and CPV strains from foxes and raccoon dogs, is wide in anti-CPV strain range and is used for clinical treatment of attacked dogs with CPV diseases, and effective rate is up to 100%.

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Номер записи: 5
22-10-2008 дата публикации

Recombination virus particles for expressing 2-typed porcine circovirus nucleocapsid protein Cap gene

Номер: CN0101289658A
Автор: HE KONGWANG, PAN QUNXING, WEN LIBIN, ZHANG XUEHAN, NI YANXIU, YU ZHENGYU, GUO RONGLI, LU LIXIN, MAO AIHUA, XIAO QI, HUANG KEHE, KONGWANG HE, QUNXING PAN, LIBIN WEN, XUEHAN ZHANG, YANXIU NI, ZHENGYU YU, RONGLI GUO, LIXIN LU, AIHUA MAO, QI XIAO, KEHE HUANG
Принадлежит:

The invention relates to construction and application of recombinant nucleocapsids of Cap genes of porcine circovirus expression type 2 nucleocapsid protein, belonging to the genetic engineering bacterin field. C-terminal gene fragments of porcine parvovirus (PPV) VP2 genes are cloned into a type 5 adenovirus shuttle vector of the human beings, and recombinant adenovirus rAd-deltaVP2 is obtained; deltaVP2 proteins are expressed successfully and highly efficiently and can be self-assembled into the nucleocapsids [PPV:VLPs]; the PPV VP2 nucleocapsids are used as antigen transport vectors and 165 to 200 sites of amino acid (deltaCap) genes of the porcine circovirus type 2 (PCV2) nucleocapsid proteins (Cap) are embedded into an N-terminal (deltaVP2) of the PPV VP2, and then recombinant adenovirus rAd-deltaCap-deltaVP2 is obtained; embedded VP2 (deltaCap-deltaVP2) proteins are expressed successfully and highly efficiently and can be self-assembled into nucleocapsids [PPV:VLP(PCV2)]. The invention ...

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Номер записи: 6