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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Применить Всего найдено 2. Отображено 2.
29-08-2012 дата публикации

Transgene cotton embryogenic callus differential culture medium

Номер: CN102648697A
Автор: Ma Yanbin, Wu Xia, Shangguan Xiaoxia, Wang Xia, Li Yane, Zhang Xiangbin, Duan Xiaokang, Han Qinglong, Ma Dongju, Cui Ting, Yuan Hui
Принадлежит:

The invention relates to the field of transgene cotton tissue culture, in particular to a transgene cotton embryogenic callus differential culture medium, which solves the problem of lower seedling rate of the existing transgene cotton embryogenic callus differentiation. A manufacture method of the culture medium comprises the following steps that: mass spectrum (MS) major elements, trace elements and glucose are added, the pH value is regulated to 6.8, curing agent Phytagel is added, and the concentration of the curing agent Phytagel is 4.0 to 4.5g/L. The transgene cotton embryogenic callus differential culture medium provided by the invention has the advantages that the concentration of the curing agent Phytagel is obviously improved, the pollution phenomenon cannot be easily generated, the growth and the development of the embryogenic callus are favorably realized, and the vitrification phenomenon of young differentiated seedlings can be effectively improved, so the embryogenic callus ...

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Номер записи: 1
30-01-2013 дата публикации

Grafting technique for excrescent buds of regenerated plants of genetically modified cotton

Номер: CN102893813A
Автор: Wu Xia, Ma Yanbin, Wang Xia, Zhang Xiangbin, Duan Xiaokang, Han Qinglong, Fei Fei, Ma Liang, Li Yan'e
Принадлежит:

The invention relates to a grafting method for plants, and particularly relates to a grafting technique for excrescent buds of regenerated plants of genetically modified cotton. According to the grafting technique, cotton seedlings of the genetically modified cotton are taken as scion plants, and cotton seedlings of non-genetically-modified cotton are taken as stock plants. The grafting method comprises the steps of preparation of the cotton seedlings, grafting, and cultivation after grafting, wherein in the preparation of the cotton seedlings in the first step, after two to three main leaves grow on the stock plant, the parts above seed leaves of the main leaves are removed, and the excrescent buds are grown between the left seed leaves and a stem; after four to six leaves grow on the excrescent buds, the top part of one excrescent bud is removed, and the top end of the left stem part of the excrescent bud is cut to be V-shaped incision; and the root part of the scion plant is cut off, ...

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Номер записи: 2