Prevention of biomass aggregation at injection wells

Disclosed herein is introduction of different nutrient solution formulations to a subterranean target site to prevent biofilm formation and biomass aggregation at the sand face of an injection well bore during MEOR or bioremediation processes. A first nutrient solution formulation supports growth of introduced microorganisms and a second nutrient solution formulation is used to promote biofilm formation to allow plugging of pores and channels in the subterranean target site, for MEOR or bioremediation.

METHODS, STRAINS, AND COMPOSITIONS USEFUL FOR MICROBIALLY ENHANCED OIL RECOVERY: PSEUDOMONAS STUTZERI

Methods, microorganisms, and compositions are provided wherein oil reservoirs are inoculated with microorganisms belonging to and medium including an electron acceptor. The grow in the oil reservoir to form plugging biofilms that reduce permeability in areas of subterranean formations thereby increasing sweep efficiency, and thereby enhancing oil recovery. 1. A method for enhancing oil recovery from an oil reservoir comprising: [{'i': 'Pseudomonas stutzeri', 'i) at least one strain of ; and'}, 'ii) a minimal growth medium comprising at least one electron acceptor;, 'a) providing a composition comprisingb) providing an oil reservoir;{'i': 'Pseudomonas stutzeri', 'c) inoculating the oil reservoir with the composition of (a) such that the populates and grows in the oil reservoir; and'}d) recovering oil from the oil reservoir;{'i': 'Pseudomonas stutzeri', 'wherein growth of the in the oil reservoir enhances oil recovery.'}2Pseudomonas stutzeri. The method of wherein the strain of comprises a 16S rDNA sequence of SEQ ID NO:8.3. The method of wherein the oil reservoir comprises at least one fluid having a concentration of salt that is at least about 30 parts per thousand.4Pseudomonas stutzeri. The method of or wherein the strain of is a strain belonging to genomovar 1 or 3.5Pseudomonas stutzeriPseudomonas stutzeri. The method of or wherein the strain of is selected from the group consisting of BR5311 (ATCC No. PTA-11283) claim 1 , 89AC1-3 (ATCC No. PTA-11284) and LH4:15 (ATCC No claim 1 , PTA-8823).6. The method of claim 1 , wherein the composition of (a) further comprises one or more additional microorganisms which grow in the presence of oil under denitrifying conditions.7Shewanella. The method of claim 6 , wherein said one or more additional microorganisms comprises a species.8Shewanella. The method of wherein the species comprises a 16S rDNA comprising the degenerate signature sequences of SEQ ID NOs: 39 claim 7 , 41 and 43.9. The method of wherein the electron ...

METHODS, STRAINS, AND COMPOSITIONS USEFUL FOR MICROBIALLY ENHANCED OIL RECOVERY: ARCOBACTER CLADE 1

Methods, microorganisms, and compositions are provided wherein oil reservoirs are inoculated with microorganisms belonging to clade 1 and medium including an electron acceptor. The strains grow in the oil reservoir to form plugging biofilms that reduce permeability in areas of subterranean formations thereby increasing sweep efficiency, and thereby enhancing oil recovery. 1. A method for enhancing oil recovery from an oil reservoir comprising: [{'i': Arcobacter', 'Arcobacter, 'i) at least one strain of belonging to clade 1; and'}, 'ii) a minimal growth medium comprising at least one electron acceptor;, 'a) providing a composition comprisingb) providing an oil reservoir;{'i': 'Arcobacter', 'c) inoculating the oil reservoir with the composition of (a) such that the containing composition populates and grows in the oil reservoir; and'}d) recovering oil from the oil reservoir;{'i': 'Arcobacter', 'wherein growth of the in the oil reservoir enhances oil recovery.'}2ArcobacterArcobacter. The method of wherein the at least one strain of belonging to clade 1 comprises the 16S rDNA degenerate consensus sequence of SEQ ID NO:40.3ArcobacterArcobacter. The method of wherein the at least one strain of belonging to clade 1 comprises a 16S rDNA sequence having at least about 97% sequence identity to a sequence selected from the group consisting of SEQ ID NOs:1 claim 1 , 2 claim 1 , 3 claim 1 , 4 claim 1 , 5 claim 1 , 6 claim 1 , 7 claim 1 , 8 claim 1 , and 39.4. The method of wherein the electron acceptor is at least one ionic salt of nitrate.5. The method of wherein the electron acceptor is at least one ionic salt of nitrite or a combination of at least one salt of nitrite and at least one salt of nitrate.6. The method of wherein the oil reservoir comprises at least one fluid having a concentration of salt of at least about 30 parts per thousand.7ArcobacterArcobacter. The method of or wherein the strain of belonging to clade 1 comprises a partial 16S rDNA sequence selected from ...

Altering the interface of hydrocarbon-coated surfaces

Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or to benefit pipeline maintenance.

Altering the interface of hydrocarbon-coated surfaces

Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or to benefit pipeline maintenance.

ALTERING THE INTERFACE OF HYDROCARBON-COATED SURFACES

Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or pipeline maintenance. 1. A method for altering the wettability of a hydrocarbon coated surface comprising:a) providing a hydrocarbon-coated surface; [{'i': 'Shewanella', 'i) a cell containing medium comprising one or more sp.; and'}, {'i': 'Shewanella', 'ii) a conditioned medium which is substantially cell free and which has been in contact with one or more sp.;'}], 'b) providing a medium selected from the group consisting ofc) contacting said hydrocarbon-coated surface with the medium of b) wherein the medium alters the wettability of said hydrocarbon-coated surface.2. The method of claim 1 , wherein the hydrocarbon-coated surface is a component of a terrestrial surface formation or subsurface formation comprising elements selected from the group consisting of rock claim 1 , soil claim 1 , brine claim 1 , sand claim 1 , shale claim 1 , clays and mixtures thereof.3. The method of claim 2 , wherein said hydrocarbon coated surface is contacted with the medium in either a producer well claim 2 , an injector well or an environmental site.4. The method of wherein the hydrocarbon coated surface is contacted with the medium by a process comprising: pumping water comprising the medium into a subsurface formation.5. The method of wherein the hydrocarbon coated surface is contacted with the medium by a process comprising; pumping water comprising the medium into a subsurface formation and allowing that water to be produced along with hydrocarbon at a producer well.6. The method of wherein the hydrocarbon coated surface is contacted with the medium by a process comprising: pumping water comprising the medium into a subsurface formation down the producer well and then back-flowing hydrocarbon and water out of the producer well.7. The method of wherein the hydrocarbon coated ...

Steady state anaerobic denitrifying consortium for application in in-situ bioremediation of hydrocarbon-contaminated sites and enhanced oil recovery

Enriched steady state microbial consortiums for microbial enhanced oil recovery and in situ bioremediation of hydrocarbon-contaminated sites, under anaerobic denitrifying conditions, are disclosed.

ALTERING THE INTERFACE OF HYDROCARBON-COATED SURFACES

Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or to benefit pipeline maintenance. 1. A method for altering the wettability of a hydrocarbon coated surface comprising:a) providing a hydrocarbon-coated surface; [{'i': 'Shewanella', 'i) a cell-containing medium comprising one or more sp.; and'}, {'i': 'Shewanella', 'ii) a conditioned medium which is substantially cell free and which has been in contact with one or more sp.;'}, {'i': 'Shewanella', 'wherein the sp. comprises a 16S rDNA comprising SEQ ID NO:21, SEQ ID NO:23 and SEQ ID NO:28; and'}], 'b) providing a medium selected from the group consisting ofc) contacting said hydrocarbon-coated surface with the medium of b) wherein the medium alters the wettability of said hydrocarbon-coated surface.2Shewanella. The method of wherein the sp. further comprises a Riboprint® pattern identifier of 212-1074-S-1 as illustrated in .3Shewanella. The method of wherein the sp. is MPHPW-1 (ATCC No. PTA-11920).4. The method of wherein the hydrocarbon-coated surface is a component of a terrestrial surface formation or subsurface formation comprising elements selected from the group consisting of rock claim 1 , soil claim 1 , brine claim 1 , sand claim 1 , shale claim 1 , clays and mixtures thereof.5. The method of wherein the subsurface formation is an oil well site comprising at least one production well and optionally at least one injection well.6. The method of wherein contacting is achieved by pumping the medium into the production well or the injector well.7. The method of wherein the medium further comprises additional components selected from the group consisting of at least one electron acceptor claim 1 , at least one additional carbon source claim 1 , and mixtures thereof.8. The method of wherein the medium further comprises at least one additional microorganism.9. A method ...

Amplification of assay reporters by nucleic acid replication

PCT No. PCT/US93/01281 Sec. 371 Date Feb. 13, 1995 Sec. 102(e) Date Feb. 13, 1995 PCT Filed Feb. 4, 1993 PCT Pub. No. WO93/15229 PCT Pub. Date Aug. 5, 1993A method for the amplified detection of an analyte, wherein amplification is achieved by replication of a target nucleic acid sequence which has been immobilized in response to analyte.

Nucleic acid fragments for the identification of dechlorinating bacteria

A unique 16S rRNA profile derived from Dehalococcoides ethenogenes has been identified and isolated. The profile contains a nucleic acid fragment that i s linked to dechlorinating activity. This sequence is set forth in SEQ ID NO:1 .

Prevention of biomass aggregation at injection wells

Disclosed herein is introduction of different nutrient solution formulations to a subterranean target site to prevent biofilm formation and biomass aggregation at the sand face of an injection well bore during MEOR or bioremediation processes. A first nutrient solution formulation supports growth of introduced microorganisms and a second nutrient solution formulation is used to promote biofilm formation to allow plugging of pores and channels in the subterranean target site, for MEOR or bioremediation.

Altering the interface of hydrocarbon-coated surfaces

Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or pipeline maintenance In one em-bodiment, the methods and compositions provided include hydrocarbon-coated surfaces that are components of a terrestrial sur-face formation or a subsurface formation

"underground target site treatment method"

A method for the amplification and detection of a nucleic acid fragment of interest

A method is provided for the replication and detection of a specific nucleic acid target using a detection probe. The probe is present throughout the amplification reaction but does not participate in the reaction in that it is not extended. The probe contains sequence complementary to the replicated nucleic acid analyte for capture of the analyte by hybridization. Additionally, the probe or analyte contains at least one reactive ligand to permit immobilization or reporting of the probe/analyte hybrid.

Identification, characterization, and application of thauera sp. al9:8 useful in microbially enhanced oil recovery

The present disclosure relates to isolation, identification and application of Thauera strain AL9:8 which grows on crude oil as the sole carbon source under denitrifying anaerobic conditions Thauera strain AL9:8 can be used alone or in concert with other microorganisms to improve oil recovery, bioremediation of oil or hydrocarbons in contaminated soil, ground water or bodies of water, such as lakes, rivers or oceans and/or applying to method for promoting oil pipeline maintenance, removing a build up hydrocarbon on the intersurfaces of the pipeline.

Identification, characterization, and application of thauera sp. al9:8 useful in microbially enhanced oil recovery

The present disclosure relates to isolation, identification and application of Thauera strain AL9:8 which grows on crude oil as the sole carbon source under denitrifying anaerobic conditions Thauera strain AL9:8 can be used alone or in concert with other microorganisms to improve oil recovery, bioremediation of oil or hydrocarbons in contaminated soil, ground water or bodies of water, such as lakes, rivers or oceans and/or applying to method for promoting oil pipeline maintenance, removing a build up hydrocarbon on the intersurfaces of the pipeline.

Prevention of biomass aggregation at injection wells.

Se describe en la presente descripción la introducción de distintas formulaciones de soluciones nutrientes a un lugar subterráneo objetivo para evitar la formación de biopelículas y agregación de biomasa en la superficie de arena de un pozo de inyección durante los procesos de MEOR o bioremediación. Una primera formulación de solución nutriente sustenta el crecimiento de los microorganismos introducidos y una segunda formulación de solución nutriente se usa para fomentar la formación de biopelículas para permitir el taponamiento de poros y canales en el lugar subterráneo objetivo, para MEOR o bioremediación.

Amplification of assay reporters by nucleic acid replication

A method for the amplified detection of an analyte, wherein amplification is achieved by replication of a target nucleic acid sequence which has been immobilized in response to analyte.

Resonant light scattering microparticle methods

Microparticle-based analytical methods, systems and applications are provided. Specifically, the use of resonant resonant light scattering as an analytical method for determining either or both a particle's identity and the presence and optionally, the concentration of one or more particular target analytes is described. Applications of these microparticle-based methods in biological and chemical assays are also disclosed.

Methods, strains, and compositions useful for microbially enhanced oil recovery: pseudomonas stutzeri.

Se proporcionan métodos, microorganismos y composiciones que se caracterizan porque los yacimientos de petróleo se inoculan con microorganismos que pertenecen a Pseudomonas stutzeri y un medio que incluye un aceptor de electrones. La Pseudomonas stutzeri crece en el yacimiento de petróleo para formar biopelículas obturadoras que reducen la permeabilidad en áreas de formaciones subterráneas e incrementan, de este modo, la eficacia de barrido y mejoran, por lo tanto, la recuperación de petróleo.

method for intensifying oil recovery, isolated microorganism and composition that intensifies oil recovery

MÉTODO PARA INTENSIFICAR RECUPERAÇÃO DE ÓLEO, MICRO- ORGANISMO E COMPOSIÇÃO. A presente invenção refere-se a métodos, micro-organismos e composições, em que reservatórios de óleo são inoculados com micro- organismos que pertencem a Arcobacter de clado 1 e um meio que inclui um aceptor de elétron. As cepas de Arcobacter crescem no reservatório de óleo para formar biofilmes de tamponamento que reduzem a permeabilidade em áreas de formações subterrâneas, aumentando, desse modo, a eficiência de varredura e intensificando, desse modo, a recuperação de óleo. METHOD FOR INTENSIFYING OIL RECOVERY, MICRO-ORGANISM AND COMPOSITION. The present invention relates to methods, microorganisms and compositions, in which oil reservoirs are inoculated with microorganisms that belong to Arcobacter clade 1 and a medium that includes an electron acceptor. Arcobacter strains grow in the oil reservoir to form buffering biofilms that reduce permeability in areas of underground formations, thereby increasing sweeping efficiency and thereby intensifying oil recovery.

Methods, strains, and compositions useful for microbially enhanced oil recovery: arcobacter clade 1

Methods, microorganisms, and compositions are provided wherein oil reservoirs are inoculated with microorganisms belonging to Arcobacter clade 1 and medium including an electron acceptor. The Arcobacter strains grow in the oil reservoir to form plugging biofilms that reduce permeability in areas of subterranean formations thereby increasing sweep efficiency, and thereby enhancing oil recovery.

Nucleic acid fragments for the identification of dechlorinating bacteria

A unique 16S rRNA profile derived from Dehalococcoides ethenogenes has been identified and isolated. The profile contains a nucleic acid fragment that is linked to dechlorinating activity. This sequence is set forth in SEQ ID NO:1.

Identification, characterization, and application of shewanella putrefaciens (lh4:18), useful in microbially enhanced oil release.

La presente descripción se relaciona con el aislamiento, la identificación y la aplicación de la cepa LH4:18 de Shewanella putrefaciens que crece bajo condiciones anaerobias desnitrificantes al petróleo crudo como única fuente de carbono. Este organismo ayuda en la liberación de petróleo del sustrato en simulaciones de reservorios, cuando se cultiva sobre lactato o peptona como fuente de carbono. La cepa LH4:18 de Shewanella putrefaciens puede usarse sola o en combinación con otros microorganismos para mejorar la recuperación de petr6leo.

method for altering the wettability of a hydrocarbon-coated surface, method for recovering oil from a reservoir, composition for improving oil recovery and shewanella sp "

Methods, strains, and compositions useful for microbially enhanced oil recovery: arcobacter clade 1

Methods, microorganisms, and compositions are provided wherein oil reservoirs are inoculated with microorganisms belonging to Arcobacter clade 1 and medium including an electron acceptor. The Arcobacter strains grow in the oil reservoir to form plugging biofilms that reduce permeability in areas of subterranean formations thereby increasing sweep efficiency, and thereby enhancing oil recovery.

Method of in situ bioremediation of hydrocarbon-contaminated sites using an enriched anaerobic steady state microbial consortium

A method for in situ bioremediation of hydrocarbon-contaminated sites using an enriched steady state microbial consortium capable of modifying crude oil components under anaerobic denitrifying conditions is disclosed.

Identification, characterization, and application of shewanella putrefaciens (lh4:18), useful in microbially enhanced oil release

The present disclosure relates to isolation, identification and application of Shewanella putrefaciens strain LH4:18 that grows, under denitrifying anaerobic conditions, on crude oil as the sole carbon source. This organism assists in oil release from substrate in reservoir simulations when grown on either lactate or peptone as a carbon source. Shewanella putrefaciens strain LH4:18 can be used alone or in concert with other microorganisms to improve oil recovery.

Nukleinsäurefragmente zur identifizierung von dechlorierenden bakterien

Et stasjonaert anaerobt denitrifiseringskonsortium for bruk i in situ bioremediasjon av hydrokarbonkontaminerte omrader og okt oljeutvinning

Anriket steady state mikrobielt konsortium mikrobiellforsterket olje-gjenvinning og in situ bioremediering av hydrokarbonkontaminerte steder, under anerobe denitrifiseringsbetingelser, er beskrevet.

Amplifikation von test reporters durch nukleinsäure replikation

Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments

A steady state anaerobic denitrifying consortium for application in in-situ bioremediation of hydrocarbon-contaminated sites and enhanced oil recovery

consórcio isolado de espécies microbianas e composição de aumento da recuperação de óleo para biorremediação in situ.

Altering the interface of hydrocarbon-coated surfaces

Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or to benefit pipeline maintenance.

Fremgangsmate for in-situ bioremediasjon av hydrokarbonkontaminerte omrader ved a anvende et anriket anaerobt stasjonaert mikrobielt konsortium

En fremgangsmåte for in situ bioremediering av hydrokarbonkontaminerte steder ved å anvende et anriket steady state mikrobielt konsortium som er i stand til å modifisere råoljekomponenter under anaerobe denitrifiseringsbetingelser er beskrevet.

Altering the interface of hydrocarbon-coated surfaces

Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or to benefit pipeline maintenance.

Methods, strains, and compositions useful for microbially enhanced oil recovery: Arcobacter clade 1

Methods, microorganisms, and compositions are provided wherein oil reservoirs are inoculated with microorganisms belonging to Arcobacter clade 1 and medium including an electron acceptor. The Arcobacter strains grow in the oil reservoir to form plugging biofilms that reduce permeability in areas of subterranean formations thereby increasing sweep efficiency, and thereby enhancing oil recovery.