02-05-2023 дата публикации
Номер: CN116042725A
Принадлежит:
The invention belongs to the technical field of agricultural biology, and relates to the technical field of silkworm breeding, in particular to a molecular breeding method of omnivorous silkworms and application. According to the invention, a silkworm gustation receptor GR43 is used as a target gene, sgRNA targeting a first exon of the silkworm gustation receptor GR43 is designed, and the gene is knocked out through a gene editing technology, so that the GR43-deleted silkworm mutant is proved to be capable of feeding various non-host plants, the recipe range is greatly widened, and the GR43-deleted silkworm mutant becomes an omnivorous silkworm; and the survival rate of the mutant bombyx mori on an artificial feed is obviously higher than that of wild bombyx mori, so that large-scale breeding of the bombyx mori is facilitated. Therefore, the omnivorous bombyx mori is obtained, the recipe of the bombyx mori is greatly broadened, and the method is very beneficial to finding non-host plants ...
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