CELLULASE VARIANTS WITH IMPROVED EXPRESSION, ACTIVITY AND STABILITY, AND USE THEREOF

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof. 1. A cellulase variant , wherein the variant is a mature form having cellulase activity and comprising a substitution at one or more positions selected from the group consisting of: 5 , 18 , 19 , 28 , 30 , 32 , 35 , 38 , 79 , 80 , 89 , 100 , 102 , 103 , 104 , 105 , 111 , 117 , 119 , 121 , 125 , 126 , 133 , 137 , 138 , 139 , 140 , 141 , 143 , 150 , 158 , 162 , 177 , 180 , 181 , 182 , 185 , 186 , 188 , 190 , 191 , 192 , 193 , 196 , 201 , 207 , 225 , 226 , 228 , 229 , 230 , 233 , 234 , 236 , 240 , 243 , 245 , 251 , 252 , 258 , 267 , 268 , 274 , 292 , 293 , 303 , 304 , 306 , 307 , 313 , 319 , 322 , 328 , 331 , 338 , 340 , 346 , 361 , 362 , 363 , 364 , 365 , 371 , 384 , 394 , 396 , 400 , 406 , 407 , 414 , 417 , 422 , 427 , 431 , 433 , 436 , 440 , 441 , 443 , 444 , 445 and 447 , wherein the positions are numbered by correspondence with the amino acid sequence of a reference cellobiohydrolase II (CBH2) set forth as SEQ ID NO:3.2. The cellulase variant of claim 1 , wherein the variant comprises a further substitution at one or more further positions selected from:(i) a first group consisting of 63, 77, 129, 146, 147, 151, 153, 157, 161, 189, 194, 197, 203, 204, 208, 211, 237, 239, 244, 247, 254, 277, 281, 285, 288, 289, 294, 327, 339, 344, 356, 378, 382 and 405; or(ii) a second group consisting of 94, 98, 107, 120, 134, 144, 154, 178, 179, 206, 210, 214, 231, 232, 266, 272, 275, 291, 312, 316, 323, 343, 360, 380, 381, 386, 399, 410, 413, 416, 426, and 429,wherein the further positions are numbered by correspondence with the amino acid sequence of the reference cellobiohydrolase II (CBH2) set forth as SEQ ID NO:3.3. A cellulase variant claim 1 , wherein said ...

Cellulase variants with improved expression, activity and stability, and use thereof

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.

Cellulase variants with improved expression, activity and stability, and use thereof

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.

UTILIZATION OF PLANT PROTEIN HOMOLOGUES IN CULTURE MEDIA

The present disclosure provides, in part, a cell culture medium supplement comprising at least one plant protein homologue of a serum protein, a cell culture medium comprising a serum-free base medium and one or more plant based proteins, and methods of growing cells in vitro and of producing cultured meat using the cell culture medium.

CELLULASE VARIANTS WITH IMPROVED EXPRESSION, ACTIVITY AND STABILITY, AND USE THEREOF

The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof. 1. A cellulase variant , wherein the variant is a mature form having cellulase activity and comprising a substitution at one or more positions selected from the group consisting of: 5 , 18 , 19 , 28 , 30 , 32 , 35 , 38 , 79 , 80 , 89 , 100 , 102 , 103 , 104 , 105 , 111 , 117 , 119 , 121 , 125 , 126 , 133 , 137 , 138 , 139 , 140 , 141 , 143 , 150 , 158 , 162 , 177 , 180 , 181 , 182 , 185 , 186 , 188 , 190 , 191 , 192 , 193 , 196 , 201 , 207 , 225 , 226 , 228 , 229 , 230 , 233 , 234 , 236 , 240 , 243 , 245 , 251 , 252 , 258 , 267 , 268 , 274 , 292 , 293 , 303 , 304 , 306 , 307 , 313 , 319 , 322 , 328 , 331 , 338 , 340 , 346 , 361 , 362 , 363 , 364 , 365 , 371 , 384 , 394 , 396 , 400 , 406 , 407 , 414 , 417 , 422 , 427 , 431 , 433 , 436 , 440 , 441 , 443 , 444 , 445 and 447 , wherein the positions are numbered by correspondence with the amino acid sequence of a reference cellobiohydrolase II (CBH2) set forth as SEQ ID NO:3.2. The cellulase variant of claim 1 , wherein the variant comprises a further substitution at one or more further positions selected from:(i) a first group consisting of 63, 77, 129, 146, 147, 151, 153, 157, 161, 189, 194, 197, 203, 204, 208, 211, 237, 239, 244, 247, 254, 277, 281, 285, 288, 289, 294, 327, 339, 344, 356, 378, 382 and 405; or(ii) a second group consisting of 94, 98, 107, 120, 134, 144, 154, 178, 179, 206, 210, 214, 231, 232, 266, 272, 275, 291, 312, 316, 323, 343, 360, 380, 381, 386, 399, 410, 413, 416, 426, and 429,wherein the further positions are numbered by correspondence with the amino acid sequence of the reference cellobiohydrolase II (CBH2) set forth as SEQ ID NO:3.36-. (canceled)7. The cellulase variant of claim 1 ...

cellulase variants with improved expression, activity and / or stability and use thereof.

Edible aerial mycelium, methods for their preparation and food products comprising the same

Provided herein are edible aerial mycelium, compositions including the same and methods for their preparation. Further provided are various food products including the aerial mycelium, such as hybrid food products containing mixtures of aerial mycelium and meat of various origin.

Utilization of plant protein homologues in culture media

The present disclosure provides, in part, a cell culture medium supplement comprising at least one plant protein homologue of a serum protein, a cell culture medium comprising a serum-free base medium and one or more plant based proteins, and methods of growing cells in vitro and of producing cultured meat using the cell culture medium. Day 3 4.5 4.0 3.5 3.0 2.5 Tw2.0 51 1.5 1.0 0.5 0.0 Organic Pea Chickpea FIG. 13