10-12-2014 дата публикации
Номер: CN104198714A
Принадлежит:
The invention provides an electrochemical immunosensor for detecting ochratoxin A. The electrochemical immunosensor comprises a work electrode, a reference electrode and a counter electrode, wherein the work electrode is prepared by that the surface of a base electrode is first modified by a carboxylated single-walled carbon nanotube/chitosan composite and then fixed with an ochratoxin A and albumin bovine serum conjugate. When the ochratoxin A is detected by the sensor, the concentration of the ochratoxin A is in a linear relationship in a range of 0.01-100 ng/ml, the linear equation is Y = 6.3155E-6-2.89458E-6X, the relevance coefficient is 0.99984, and the minimum detection limit is 0.004 ng/ml. Compared with the prior art, the sensor has the advantages of high sensitivity, great specificity, low cost, simplicity and convenience for operation and short detection period, is suitable for the measurement of actual samples and is expected to have actual application values.
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