27-04-2017 дата публикации
Номер: US20170114149A1
Принадлежит:
Disclosed are methods of facilitating the interaction of a first and a second component, the method including the use of an antibody fragment and an epitope tag. The antibody fragment may be bound to a first component, while the epitope tag may be bound to a second component. The antibody fragment may have a binding specificity for the epitope tag sufficient to cause an interaction between the antibody fragment and the epitope tag. 2. The method of wherein said antibody fragment and said epitope tag transiently interact.3. The method of wherein said antibody fragment and said epitope tag transiently interact claim 1 , wherein said transient interaction occurs in the interior of a cell.4. The method of wherein said antibody fragment is covalently bound to said first component.5. The method of wherein said antibody fragment comprises a single chain variable fragment (ScFv).6. The method of wherein said antibody fragment comprises a single chain variable fragment (ScFv) claim 1 , a single domain antibody (sdAb) claim 1 , a domain antibody claim 1 , a SMIP claim 1 , or a combination thereof.7. The method of wherein said first and second component comprise an epitope tag covalently attached to target protein and a ScFv covalently attached to a signal.8. The method of wherein said first component is selected from a protein claim 1 , a small molecule claim 1 , a fluorophore claim 1 , a signal peptide claim 1 , a nanoparticle claim 1 , a cellular component claim 1 , and combinations thereof.9. The method of wherein said first component comprises an effector molecule.10. The method of wherein said first component comprises an effector molecule selected from a transcription factor (activator or repressor) claim 1 , chromatin remodeling factor claim 1 , exonuclease claim 1 , endonuclease claim 1 , transposase claim 1 , methytransferase claim 1 , demethylase claim 1 , acetyltransferase claim 1 , deacetylase claim 1 , kinase claim 1 , phosphatase claim 1 , integrase claim 1 , ...
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