Method for inducing nematode-trapping fungi to synchronously produce trapping organs

The invention relates to a method for inducing nematode-trapping fungi to synchronously produce trapping organs, which belongs to the field of applied microbiology. The method comprises the steps of culture of nematodes, preparation of a nematode extract, culture of nematode-trapping fungi and induction of trapping organs. The method can utilize the nematode extract to induce the nematode-trapping fungi to synchronously produce a large number of trapping organs (160 trapping organs/mg mycelium) by culturing the nematodes (Caenorhabditis elegans) and preparing the nematode extract by utilizing ultrasonic waves for crushing and centrifugalizing. The method has the advantages of simple and easy operation and good repeatability, and can obtain a large number of synchronous three-dimensional fungal nets, thereby providing good experimental materials for further research of the molecular mechanism of forming the trapping organs of the nematode-trapping fungi.

Nematophagous fungi infectious extracellular serine proteinase crystal morphology

The invention relates to an extracellular serine protease crystal structure with nematophagous fungi infectivity and belongs to the fields of molecular biology and applied microbiology. The invention implements liquid cultivation with Lecanicillium psalliotae YMF1.00112 and Paecilomyces lilacinus M-14, fractional precipitation of ammonium sulphate with a fermented liquid, and dissolution of protease-containing active component with buffer solution, and then obtains raw enzyme solution. Cation exchange chromatography and molecular sieve purification of the raw enzyme solution are implemented. After purified enzyme is obtained, concentration processing is implemented so as to obtain an enzyme solution with higher concentration. Hanging drop vapor diffused crystallization of the enzyme solution with higher concentration is implemented. The crystallization conditions are filtered with a reagent kit. Enough diffraction pictures of obtained crystal are collected through diffraction on an X-ray ...

Heteromorphic Dactlella bacterial agent capable of killing nematode and application thereof