Monacrosporium elegans bactericidal agent with nematode killing function and its preparation method and use thereof

This invention relates to a Monacrosporium elegans bacteriostatic and its process and application, belonging to microbial pesticide technology region, with the preparing bacterial strain being Monacrosporium elegans, YMF1.00027, adopting normal process to prepare. Wherein, liquid fermenting culture medium dispense includes: 0.1% of glucose, 0.1% of ammonia sulfate, 0.05% of addex-magnesium, and liquor boiled from 100g potatoes, 0.001% of copperas, and filling waters to complement volume to 1L, PH6.5. The method for extracting effective composition to kill nemathelminth from liquid fermenting production is characterized by filtering fermented culture and dewatering supernatant with ammonia sulfate. The bacteriostatic is effective for killing Panagrellus redivivus and pine beam nemathelminths, and has broad aspect of application.

Acinetobacter and application thereof

The invention discloses an Acinetobacter and application thereof. Production bacterial strain liquid is fermented and cultured to form corresponding preparation. The Production bacterial strain is the Acinetobacter (Acinetobacter sp.ND12); and the bacterial strain was preserved in China General Microbiological Culture Collection Center on November 5, 2009, and a preservation number is CGMCC No.3410. The bacterial strain has stronger nicotine degradation capacity and nicotine toxicity resistance capacity, and can be grown at the nicotine concentration of 3.5g/L; and by large-scale culturing of the bacterial strain, the pure nicotine can be degraded by using a whole cell of the bacterial strain as a biocatalyst, and meanwhile the content of the nicotine in tobacco is also reduced. The Acinetobacter and the application thereof have the advantages of capacities of adjusting the content of the nicotine in the tobacco raw material, improving availability of the tobacco, degrading the nicotine ...

Method for inducing nematode-trapping fungi to synchronously produce trapping organs

The invention relates to a method for inducing nematode-trapping fungi to synchronously produce trapping organs, which belongs to the field of applied microbiology. The method comprises the steps of culture of nematodes, preparation of a nematode extract, culture of nematode-trapping fungi and induction of trapping organs. The method can utilize the nematode extract to induce the nematode-trapping fungi to synchronously produce a large number of trapping organs (160 trapping organs/mg mycelium) by culturing the nematodes (Caenorhabditis elegans) and preparing the nematode extract by utilizing ultrasonic waves for crushing and centrifugalizing. The method has the advantages of simple and easy operation and good repeatability, and can obtain a large number of synchronous three-dimensional fungal nets, thereby providing good experimental materials for further research of the molecular mechanism of forming the trapping organs of the nematode-trapping fungi.

Application of cystic fibrosis transmembrane transduction regulating factor inhibitor to preparation of medicaments for treating diabetes

The invention relates to application of cystic fibrosis transmembrane transduction regulating factor inhibitor to the preparation of medicaments for treating diabetes, and application of thiazolidone compounds or derivatives thereof to the preparation of medicaments for treating diabetes. The cystic fibrosis transmembrane transduction regulating factor inhibitor can be subjected to compatibility with medicaments of sulfonylurea, glinides, biguanides, insulin sensitizers and glycosidase inhibitors.

A strain of Rhodococcus capable of metabolizing nicotine and use thereof

The invention relates to a rhodococcus that can metabolize nicotine and application thereof, and belongs to the technical field of applied microbiology. The rhodococcus (Rhodococcus sp.Y22) is preserved in General Microbiology Center of China Microbial Culture Collection Management Committee on September 28th, 2008 with the preservation number CGMCC No. of 2683. The rhodococcus (Rhodococcus sp.Y22) has stronger nicotine metabolizing capability and nicotine toxicity resisting capability, and can grow at high nicotine concentration of 3 grams per liter. Through the cultivation of abundant stains, integral cells of the strains can be used as a biological catalyst for completely degrading pure nicotine and simultaneously decreasing the nicotine content in tobaccos. The rhodococcus has the advantages of adjusting the nicotine content in tobacco materials, improving tobacco usability, decomposing nicotine in smoke dust and reducing environmental pollution and the like.

Acinetobacter and application thereof

The invention discloses an Acinetobacter and application thereof. Production bacterial strain liquid is fermented and cultured to form corresponding preparation. The Production bacterial strain is the Acinetobacter (Acinetobacter sp.ND12); and the bacterial strain was preserved in China General Microbiological Culture Collection Center on November 5, 2009, and a preservation number is CGMCC No.3410. The bacterial strain has stronger nicotine degradation capacity and nicotine toxicity resistance capacity, and can be grown at the nicotine concentration of 3.5g/L; and by large-scale culturing of the bacterial strain, the pure nicotine can be degraded by using a whole cell of the bacterial strain as a biocatalyst, and meanwhile the content of the nicotine in tobacco is also reduced. The Acinetobacter and the application thereof have the advantages of capacities of adjusting the content of the nicotine in the tobacco raw material, improving availability of the tobacco, degrading the nicotine ...

Bacteria ferulic acid decarboxylase gene and crystal structure thereof

The invention relates to an expression, purification, crystallization method, structure elucidation and the field of application technology of forulic acid decarboxylase, which belongs to the field of molecular biology and microbiology application. The invention obtains the coding area sequence of the key enzyme forulic acid decarboxylase which decomposes forulic acid to generate 4-vinyl guaiacol in Enterobacter sp.Px6-4 by the gene clone method, expresses the enzyme in a large amount in the colon bacillus ( E.coli BL21 ) by the heterogenous expressing technology, obtains the forulic acid decarboxylase pure products by the purification method and the forulic acid decarboxylase protein crystallization by the hanging-drop crystallization process, and grasps the structure and active centre of the enzyme. The invention can obtain a large amount of highly active forulic acid decarboxylase, and provide theoretical guidance for reconstructing engineering bacteria and improving the output of 4- ...

Method for inducing nematode-trapping fungi to produce capturing devices through amino acid

The invention relates to a method for inducing nematode-trapping fungi to produce capturing devices through amino acid, and belongs to the field of microbiology application. The method comprises the steps of preparing an amino acid solution, cultivating the nematode-trapping fungi, collecting spores, soaking the spores in the amino acid solution and inducing the capturing devices. The method includes the specific steps that a, the amino acid used in amino acid solution preparation is valine or leucine or glutamic acid; b, conidia of the nematode-trapping fungi are adopted in the inducing step of the capturing devices, and the capturing devices are induced in a soaking mode through the amino acid solution. The method for inducing the nematode-trapping fungi to produce the capturing devices through the amino acid has the advantages that the spores of the nematode-trapping fungi are cultivated and collected, the valine or the leucine or the glutamic acid is used for soaking the spores, and ...

Crude enzyme liquid prepared by microorganism and uses thereof

Cystic monacrosporium janus prepn with nematocide function and its preparing method and use

The present invention belongs to the field of microbial pesticide technology. The Monacrosporium cystosporium preparation with nenmatocide function is prepared with the strain of Monacrosporium cystosporium YMF1.00024 and through conventional process. The liquid fermentation culture medium contains glucose 0.1 wt%, ammonium sulfate 0.1 wt%, magnesium sulfate 0.05 wt%, boiled potato juice 10 wt% and ferrous sulfate 0.001 wt% except water, and has pH 6.5. The liquid fermented matter is filtered and the supernatant is concentrated with ammonium sulfate to form the nenmatocide liquid. The product of the present invention has the outstand feature of high toxicity to Panagrellus redivivus nematode and good latent application foreground.

Bacillus pumilus and application thereof

The invention discloses bacillus pumilus and application thereof, which belong to the technical field of microbes. The produced strain of the invention is named Bacillus pumilus Van35 according to classification, and the preservation number of the strain is CGMCC No.3411. When the strain preparation is spayed, the soluble total sugar content and the reducing sugar content of tobaccos can be increased, while protein, total nitrogen and nicotine content are reduced. Therefore, specific values of the ratio of dextrose to nitrogen, the ratio of total sugar to betaine and the ratio of total nitrogen to total alkaloid tend to rise, the quality of chemicals is high and the quality of the smoke panel test is improved.

Spice extractum prepared by waste tobacco leaf, preparation method thereof and application thereof

The invention discloses a spice extractum prepared by waste tobacco leaf, a preparation method thereof and application thereof. The preparation method comprises the steps of: taking the waste tobacco leaf as a raw material; sequentially fermenting acinetobacter, bacillus pumilus and bacillus subtilis to reduce nicotine in the waste tobacco leaf; transforming ferulic acid in the tobacco leaf to generate flavor ingredients such as the 4-guaethol, the vanillin and the like; and extracting the flavor ingredients in the waste tobacco leaf after fermenting to prepare the spice extractum. The spice extractum comprises higher-content flavor ingredients which are mainly to be benzyl alcohol, solanone, vanillin, BETA-damascenone, dihydro kiwifruit lactone, megastigmatrienone, duvatriene diol and neophytadiene. The result of the aromaticflvouring smoke panel test shows that the spice extractum has the effects of obviously dispatching the tobacco aroma, fining and smoothing the alcohol and the smoke ...

Natural vanillin composite perfume and uses thereof

The invention relates to a natural vanillin composite aroma material and application thereof, pertaining to the microbial application technical field. The aroma can be obtained from producing strain and auxiliary materials after an amplification culture of liquid, liquid fermentation and extracting separation; wherein the producing strain thereof belongs to Enterobacter sp.Px6-4 and the strain has been preserved at CGMCC since April 12, 2007 with the preserving number of CGMCC No. 1999. The aroma substance of vanillin composite aroma materials and the corresponding percentage content respectively are: vaniilin 0.69%, 4-ethyl group guaialcol 0.75%, 4-vinyl guaialcol 70.31%, 4-acetyl guaialcol 0.08%, 4-propyl guaialcol 0.09%, 4-guaialcol 1.63%, 1-butyl alcohol 0.34%, 3-hydroxyl groups-2-butanone 0.05%, unknown material with undetermined structure 6.34%. The vanillin composite aroma material is suitable for perfume cigarette application. The vanillin composite aroma material of the invention ...

A strain of Rhodococcus capable of metabolizing nicotine and use thereof

The invention relates to a rhodococcus that can metabolize nicotine and application thereof, and belongs to the technical field of applied microbiology. The rhodococcus (Rhodococcus sp.Y22) is preserved in General Microbiology Center of China Microbial Culture Collection Management Committee on September 28th, 2008 with the preservation number CGMCC No. of 2683. The rhodococcus (Rhodococcus sp.Y22) has stronger nicotine metabolizing capability and nicotine toxicity resisting capability, and can grow at high nicotine concentration of 3 grams per liter. Through the cultivation of abundant stains, integral cells of the strains can be used as a biological catalyst for completely degrading pure nicotine and simultaneously decreasing the nicotine content in tobaccos. The rhodococcus has the advantages of adjusting the nicotine content in tobacco materials, improving tobacco usability, decomposing nicotine in smoke dust and reducing environmental pollution and the like.

Modified polyolefin plastic

The invention discloses a modified polyolefin plastic. The modified polyolefin plastic is prepared from the following components in percentage by weight: 10%-15% of polyolefin, 70%-75% of calcium carbonate filler, 5%-6% of an assistant and 5%-6% of a modifier, wherein the calcium carbonate filler, taking limestone as a raw material, is prepared through the steps of mixing with a high-speed mixer at the temperature of 120-150 DEG C, plasticizing, and granulating. The invention also discloses a degradable modified polyolefin plastic. The degradable modified polyolefin plastic is prepared from the following components in percentage by weight: 10%-15% of polyolefin, 70%-75% of calcium carbonate filler, 4%-5% of an assistant, 4%-5% of a modifier and 0.5%-2% of a degrading agent, wherein the calcium carbonate filler, taking limestone as a raw material, is prepared through the steps of mixing with a high-speed mixer at the temperature of 120-150 DEG C, plasticizing, and granulating. The modified ...

Crude enzyme liquid prepared by microorganism and uses thereof

The invention relates to a crude enzyme produced by microorganism and an application thereof, pertaining to the microorganism application technical field. The invention is obtained by producing strainand auxiliary material through liquid enlarging culture and liquid fermentation culture; the producing strain is pseudomonas sp which is preserved in a common microorganism center of China Committeefor Culture Collection of microorganisms on April 12, 2007, and the preservation number is: CGMCC No.1998. The crude enzyme prepared by the microorganism of the invention can be used for reducing thecontent of the nicotine in tobacco. The invention has the advantages of being capable of adjusting the nicotine content in tobacco leaf material, enhancing the usability of tobacco life and degradingthe nicotine in the smoke and reducing the pollution to environment, etc.

Nematophagous fungi infectious extracellular serine proteinase crystal morphology

The invention relates to an extracellular serine protease crystal structure with nematophagous fungi infectivity and belongs to the fields of molecular biology and applied microbiology. The invention implements liquid cultivation with Lecanicillium psalliotae YMF1.00112 and Paecilomyces lilacinus M-14, fractional precipitation of ammonium sulphate with a fermented liquid, and dissolution of protease-containing active component with buffer solution, and then obtains raw enzyme solution. Cation exchange chromatography and molecular sieve purification of the raw enzyme solution are implemented. After purified enzyme is obtained, concentration processing is implemented so as to obtain an enzyme solution with higher concentration. Hanging drop vapor diffused crystallization of the enzyme solution with higher concentration is implemented. The crystallization conditions are filtered with a reagent kit. Enough diffraction pictures of obtained crystal are collected through diffraction on an X-ray ...

Wireworm-killing biologic bacterial agent, its preparation and application

The present invention relates to a process for preparing and application of bionematocide and belongs to technique domain of biological pesticide. Product of the invention can be gained from conventional method that the produced strain is cultivated in test tube, enlarged cultivated in liquid and extracts active constituents of nematocide, whose produced strain is Lecanicillium psalliotae YMF1.00112, with conservation number CGMCC No.1312. The formulation of enlarged liquid base in the invention is, dextrose 10g, ammonia sulfate 1g, magnesium sulfate 1g, ferrous sulfate 0.015g, leachate of potato 1000mg, pH6.5. the invention can poison pine wood nematode and Panagrellus redivivus and be used as bio-prevention to plant parasitic nematode. The product has the advantage of high poison effect, safety to human body and environment protection, etc.

Method for promoting nematode-trapping fungi to generate conidia by utilizing sodium nitrate

The invention relates to a method for promoting nematode-trapping fungi to generate conidia by utilizing sodium nitrate, and belongs to the field of applied microbiology. The method comprises the following steps: preparing a culture medium; culturing the nematode-trapping fungi; collecting and counting the conidia. The method disclosed by the invention can be used for promoting the nematode-trapping fungi to generate the conidia by adding 0.2% NaNO3 to a CMA (Corn Meal Agar) culture medium. Compared with the traditional method, the method disclosed by the invention has the advantages of outstandingly increasing the quantity of the conidia obtained through the method more than 4 times maximally, being easy to operate and good in repeatability, obtaining a large quantity of the conidia and further laying a good foundation for further researching and utilizing the nematode-trapping fungi as biocontrol bacteria of pathogen nematodes.

Natural vanillin composite perfume and uses thereof

The invention relates to a natural vanillin composite aroma material and application thereof, pertaining to the microbial application technical field. The aroma can be obtained from producing strain and auxiliary materials after an amplification culture of liquid, liquid fermentation and extracting separation; wherein the producing strain thereof belongs to Enterobacter sp.Px6-4 and the strain hasbeen preserved at CGMCC since April 12, 2007 with the preserving number of CGMCC No. 1999. The aroma substance of vanillin composite aroma materials and the corresponding percentage content respectively are: vaniilin 0.69%, 4-ethyl group guaialcol 0.75%, 4-vinyl guaialcol 70.31%, 4-acetyl guaialcol 0.08%, 4-propyl guaialcol 0.09%, 4-guaialcol 1.63%, 1-butyl alcohol 0.34%, 3-hydroxyl groups-2-butanone 0.05%, unknown material with undetermined structure 6.34%. The vanillin composite aroma material is suitable for perfume cigarette application. The vanillin composite aroma material of the invention ...

Crystal morphology of Nematophagous fungi infectious extracellular chitinase

The invention relates to a crystal structure of a nematophagous fungi infectivity extracellular chitinase, which belongs to the fields of molecular biology and applied microbiology. In the invention, a cDNA sequence the introne of which is removed is obtained through the cloning of a reverse transcription PCR and is expressed in colon bacillus E.coli. The pure product of the chitinase is obtained through a protein purifier; the protein crystal of the chitinase is obtained by applying a hanging drop dispersing method; and moreover, the compound crystal of the chitinase and caffeine is obtained through a method of substrate dipping. The structures of the compound crystal of the chitinase and the compound crystal are resolved through a method of X-diffraction. The data of the crystal structures can fully reflect the amino acid space conformation of the chitinase and provides theoretical instructions for further researching the relation between the structure and the function of the chitinase ...

Complex enzyme additive for tobacco and preparation method and application thereof

The invention discloses a complex enzyme additive for tobacco, a preparation method and application thereof, and belongs to the technical field of tobacco additives. The complex enzyme additive (FHM) is prepared by utilizing the conventional commercialized enzyme preparation product and combining main chemical components of the tobacco through experimental results of tobacco treatment by single enzymes in an appropriate proportion. Biological enzymes are subjected to orthogonal experimental design according to five factors, and different classes of every variety are screened for an optimal combination by a method for screening the single enzymes. A formula of the screened optimal combination can be used for an artificial alcoholization fermentation experiment of the tobacco. By the complex enzyme additive, the qualities such as an aroma amount, pleasure and the like of middle and lower levels of upper tobacco leaves and middle tobacco leaves are remarkably improved, miscellaneous gas, irritation ...

Arthrobotrys oligospora genetic engineering strain with nematode-killing function, and application thereof

The invention relates to a nematode-trapping fungus of arthrobotrys oligospora genetic engineering strain with a nematode-killing function, and an application thereof, and belongs to the technical field of biological pesticides. A production strain provided by the invention is arthrobotrys oligospora genetic engineering strain (arthrobotrys oligospora [Delta]Mad1), with a preservation number of CCTCC M2013439. The arthrobotrys oligospora genetic engineering strain is applied in preparing a preparation for preventing and controlling nematode. The arthrobotrys oligospora genetic engineering strain has the same growth rate and form with a starting strain, and the difference between the arthrobotrys oligospora genetic engineering strain and a wild strain lies in that the strain can produce a viscous three-dimensional bacterium net two times higher than that of the starting strain when the strain is cultured in a water agar medium containing 0.1%-0.5% of sodium nitrate, or 0.1%-0.5% of urea ...

Discharging device for concrete and mortar processing and stirring

The utility model discloses a discharging device for processing and stirring concrete and mortar. The device comprises a base, a first motor is fixedly connected to the top of the base, a driving wheel is fixedly connected to an output shaft of the first motor through a coupler, a conveying belt is movably connected to the outer wall of the driving wheel, a conveying box is fixedly connected to the outer wall of the conveying belt, and a fixing rod is fixedly connected to the top of the base. The utility model discloses a discharging device for processing and stirring concrete and mortar. Rawmaterials can be poured into the discharging device to be stirred without manually lifting and transporting sand, stone and cement; the purposes of improving the working efficiency and being convenient to use are achieved; the problem that a common concrete and mortar processing and stirring discharging device needs to consume a large amount of labor to convey raw materials into the device is avoided ...

Heteromorphic Dactlella bacterial agent capable of killing nematode and application thereof