Method for preparing ferric lithium phosphate precursor by comprehensive utilization of ilmenite

The invention discloses a preparation method for lithium iron phosphate precursor by comprehensively utilizing ilmenite, comprising a plurality of steps: leach the ilmenite in acid and filtrate is obtained by filtering; dissolve a certain amount of other iron sources in the filtrate to make the Fe concentration in the mixed solution as 0.01-3mol/L and the molar ratio of Ti and Fe as 0.0005-0.5; add appropriate amount of oxidant to the mixed solution; use alkali aqueous solution to regulate the system pH as 4.0-14.0; react for 10min to 24h at 10-90 DEG Cin a stir reactor; filter, wash and dry the sedimentation at 50-159 DEG C; after drying, calcine the sedimentation in air at 300-800 DEG C for 1-24h; lithium iron phosphate precursor for the lithium-ion battery cathode material, which is also the ferric oxide of doped-metal elements, can be obtained. The preparation method has the advantages of wide material source, simple technological process, good and stable product quality and low cost ...

Afterheat recycling method and device thereof in enamelled wire production

The invention relates to afterheat recycling method and device thereof in enamelled wire production. The device comprises two heat collectors which are respectively a heat collecting pipe and a coil, and the heat collecting pipe and the coil finish twice afterheat collections and twice afterheat transmissions respectively through two heat transmission pipelines, wherein the quantities of heat transmitted each time are different and respectively applied to heating processes of different degrees of an annealing furnace so as to ensure that the use of electric energy of the annealing furnace originally required to be electrically heated is reduced, thereby greatly improving the utilization of the afterheat and reduce the emission of pollutions.

Method for preparing ferric lithium phosphate precursor by comprehensive utilization of ilmenite

The invention discloses a method for comprehensively utilizing titanic iron ore to prepare a precursor of the lithium iron phosphate, which is characterized in that titanium iron ore is immerged into an acid, filtering, a quantitative other ore resource is dissolved in the filtered solution, making the Fe concentration in the mixed solution to be from 0.01 to 3 mol/L and the molar ration of Ti to Fe to be from 0.0005 to 0.3; an oxidant and a sedimentation agent are put into the solution, wherein the concentration of the oxidant and the sedimentation agent is from 0.01 to 3 mol/L, using an alkaline solution from 0.01 to 6 mol/L to control pH value of the system between 1.5 and 6.0, reacting for from 5 minutes to 24 hours in an agitating reactor at a temperature of between 20 and 90 degree centigrade, the attained sedimentation is rinsed and filtered, the precursor of the lithium iron phosphate for the anode material of a lithium-ion battery is got after the attained sedimentation continues ...

Afterheat recycling method and device thereof in enamelled wire production

The invention relates to afterheat recycling method and device thereof in enamelled wire production. The device comprises two heat collectors which are respectively a heat collecting pipe and a coil, and the heat collecting pipe and the coil finish twice afterheat collections and twice afterheat transmissions respectively through two heat transmission pipelines, wherein the quantities of heat transmitted each time are different and respectively applied to heating processes of different degrees of an annealing furnace so as to ensure that the use of electric energy of the annealing furnace originally required to be electrically heated is reduced, thereby greatly improving the utilization of the afterheat and reduce the emission of pollutions.

Method for improving nickel cobalt leaching rate from lateritic nickel at low cost

The invention provides a low-cost method for improving the nickel-cobalt leaching rate of low-grade lateritic nickel ore. In the method, laterite ore is roasted twice, wherein primary roasting is hermetically carried out for about 30 minutes at a temperature between 90 and 110 DEG C, and secondary roasting is carried out for about 1 hour at a temperature between 260 and 420 DEG C under the condition with air supply, so as to change the phase structure of nickel-cobalt-containing metal in mineral, ensure that the nickel-cobalt-containing metal is easier to leach by leaching agents and raise the nickel-cobalt leaching rate with low acid consumption at a low temperature. At the same time of roasting, the method changes the existence structure of iron, increases leaching activation energy, and reduces the leaching of iron. A roasting material is cooled to about 50 DEG C in air, and leaching is carried out by adding sulfuric acid or hydrochloric acid at about 50 DEG C. The leaching rate ofnickel ...

Method for preparing lithium titanate precursor and lithium iron phosphate precursor by comprehensively utilizing ilmenite

The invention discloses a method for preparing a lithium titanate precursor and a lithium iron phosphate precursor by comprehensively utilizing ilmenite. The method is characterized by comprising the following steps of: 1) separating ferrotitanium: performing ball milling on the ilmenite, leaching the ilmenite with sulfuric acid, cooling and filtering the leached ilmenite to obtain filter residue and filtrate; 2) preparing a lithium titanate precursor: washing the obtained filter residue with dilute sulfuric acid, dissolving the filter residue in 75 to 90 percent sulfuric acid, diluting the mixture, adding a precipitator into the mixture for reaction, cooling, standing and filtering the mixture, and drying the filter residue to obtain the precursor of the lithium titanate; and 3) preparing the lithium iron phosphate precursor: diluting the filtrate, adding oxidant and precipitator into the solution, controlling the pH value of the system with aqueous solution of alkali, and washing, filtering ...

Method for preparing lithium iron phosphate precursor comprehensively from laterite type nickel ores

The invention discloses a method for preparing a lithium iron phosphate precursor comprehensively from laterite type nickel ores, which comprises: using an acid to fully leach out the laterite type nickel ores, and driving the Fe concentration in lixivium to between 0.01 and 6 mol/L; adding 0.01 to 9 mol/L oxidant and precipitator into the lixivium, using 0.01 to 6 mol/L of complexant or surfactant to control the appearance, and using 0.01 to 6 mol/L of aqueous alkali to control the pH value of the system to between 0.1 and 3; and performing reaction on the system in a stirring reactor at the temperature of between 20 and 90 DEG C for 5 minutes to 24 hours, washing and filtering the obtained deposit, drying the obtained product at the temperature of between 50 and 200 DEG C, and obtaining the precursor of a lithium iron phosphate anode material of a lithium ion battery. The invention has the characteristics of wide source of raw materials, simple technological process, good product quality ...

Chinese medicine composition for treating hypertension and preparation method thereof

The invention discloses a Chinese medicine composition for treating hypertension and a preparation method thereof, belonging to the field of Chinese medicines. The Chinese medicine composition comprises bulk pharmaceutical chemicals of gastrodia tuer, uncaria rhynchophylla, the bark of eucommia and the like. The bulk pharmaceutical chemicals are distilled with steam, decocted with water, extracted with alcohol and the like respectively according to different characteristics for extracting effective components and prepared into a capsule, a tablet, a granular formulation, powder and a pill. The Chinese medicine composition has the functions of calming liver wind and calming and reducing blood pressure and is mainly used for treating the liver-yang hyperactivity type hypertension.

Moulding mould special for bridge precast beam plate

The invention relates to a moulding mould special for a bridge precast beam plate. The moulding mould special for the bridge precast beam plate comprises a core model, an external template and a pedestal, wherein the external template comprises side templates and end templates; the lower ends of two parallel groups of side templates are hinged with front and rear sides of the pedestal respectively; a first oil cylinder for driving the side template to rotate around a hinged joint is arranged on the outside of each side template; left and right groups of end templates are positioned between the two groups of side templates above the pedestal respectively; the core model runs through the left and right groups of end templates; and a second oil cylinder for driving the end template to slide leftwards and rightwards is arranged on the outside of each end template. The moulding mould special for the bridge precast beam plate has the advantages of simple structure, convenient manufacturing, improvement ...

Metal resin anchoring rod body automatic thread-rolling conveyor

The invention discloses a metal resin anchoring rod body automatic thread-rolling conveyor, which belongs to the automatic thread-rolling conveyor. A rolling-over feeding mechanism, a chain conveying mechanism, a pneumatic leveling mechanism, a thread-rolling pushing mechanism and a rod throwing mechanism of the automatic thread-rolling conveyor are orderly connected to a frame; sensors are connected to the rolling-over feeding mechanism, the chain conveying mechanism, the pneumatic leveling mechanism, the thread-rolling pushing mechanism and the rod throwing mechanism respectively and are all connected with a PLC; the output end of an air compressor is connected with the rolling-over feeding mechanism, the pneumatic leveling mechanism, the thread-rolling pushing mechanism and the rod throwing mechanism; and a motor is connected with the chain conveying mechanism. The automatic thread-rolling conveyor has the advantages of improving machining accuracy and consistency, improving labor productivity ...

Moulding mould special for bridge precast beam plate

The invention relates to a moulding mould special for a bridge precast beam plate. The moulding mould special for the bridge precast beam plate comprises a core model, an external template and a pedestal, wherein the external template comprises side templates and end templates; the lower ends of two parallel groups of side templates are hinged with front and rear sides of the pedestal respectively; a first oil cylinder for driving the side template to rotate around a hinged joint is arranged on the outside of each side template; left and right groups of end templates are positioned between the two groups of side templates above the pedestal respectively; the core model runs through the left and right groups of end templates; and a second oil cylinder for driving the end template to slide leftwards and rightwards is arranged on the outside of each end template. The moulding mould special for the bridge precast beam plate has the advantages of simple structure, convenient manufacturing,improvement ...

Method for the preparation of high rate lithium ion capacitor battery cathode material by pyrolyzing asphalt at low temperature to cover graphite

The invention relates to a method for the preparation of high rate lithium ion capacitor battery cathode material by pyrolyzing asphalt at low temperature to cover graphite, belonging to the technical field of the preparation of energy storage material. The invention comprises the steps that: first, mixed solution containing graphitizing catalyst, water and alcohol is formulated, then graphite, asphalt powder and conductive agent are added therein for being mixed for several hours, the resultant mixture is dried in an atmosphere of the air, the asphalt is then pyrolyzed in an atmosphere of Ar or N2, and in the end, salpeter solution is used to remove the graphitizing catalyst by means of dissolution, and the high rate lithium ion capacitor battery cathode material with pyrolyzed asphalt covering the graphite is obtained by sufficient washing and drying. The cathode material obtained according to the method has the characteristics of high capacity and good high rate performance, is simple ...

Method for producing battery-grade ferrous oxalate by using ferrous sulfate as titanium pigment byproduct

The invention discloses a method for producing battery-grade ferrous oxalate by using ferrous sulfate as a titanium pigment byproduct. The waste and byproduct ferrous sulfate is dissolved in deionizedwater; the mixture is filtered; a complexing agent, a surfactant and a precipitation agent are added according to a certain order into the filtrate when violent stirring is carried out; then, aqueoussolution of alkaline or acid is used for controlling the pH of the system to be 0.1-5.0, the stirring is not stopped until reaction is carried out at temperature of 20-80 DEG C for 5 min-4 h, aging is carried out for 0-24 h; the obtained sediment is filtered, washed and dried so as to obtain the battery-grade ferrous oxalate, wherein the iron recovery rate can be as high as 99.9 percent. By adjusting the addition order and the addition amount of the additives as well as the pH value of the system, the method causes the types and the amount of metallic elements in the ferrous oxalate to be controllable ...

Method for comprehensive exploitation of low-ore grade laterite nickel mine

The invention discloses a method for comprehensively developing low-grade nickeliferous laterite ore. The main technique comprises the steps of the preparation of minerals, chlorination and leaching, the oxidation of leachate, the regeneration of hydrochloric acid and the hydrolysis of settled iron, solid-liquid separation, sulfuration and sediment and the recycling of chloride and the like; the method is characterized in that the nickeliferous laterite ore is leached out by hydrochloric acid and the chloride at normal pressure and iron in the nickeliferous laterite ore is leached out as muchas possible; leached ferrous ions are oxidized into ferric ion; the regeneration of hydrochloric acid and hydrolysis of settled iron are realized synchronously under the condition of normal pressure and the temperature of 140 to 180 DEG C; the collection of the regenerated hydrochloric acid prompts the hydrolysis reaction to be complete so as to obtain iron oxide red of byproduct; and after solid-liquid ...

Method for comprehensive exploitation of low-ore grade laterite nickel mine

The invention discloses a method for comprehensively developing low-grade nickeliferous laterite ore. The main technique comprises the steps of the preparation of minerals, chlorination and leaching, the oxidation of leachate, the regeneration of hydrochloric acid and the hydrolysis of settled iron, solid-liquid separation, sulfuration and sediment and the recycling of chloride and the like; the method is characterized in that the nickeliferous laterite ore is leached out by hydrochloric acid and the chloride at normal pressure and iron in the nickeliferous laterite ore is leached out as much as possible; leached ferrous ions are oxidized into ferric ion; the regeneration of hydrochloric acid and hydrolysis of settled iron are realized synchronously under the condition of normal pressure and the temperature of 140 to 180 DEG C; the collection of the regenerated hydrochloric acid prompts the hydrolysis reaction to be complete so as to obtain iron oxide red of byproduct; and after solid-liquid ...

Method for preparing ferric lithium phosphate precursor by comprehensive utilization of ilmenite

New ring-focus phosphate compounds and preparation method thereof

The invention discloses new ring-focus phosphate compounds and a preparation method thereof. The ring-focus phosphate compounds provided by the invention have a structure of the general formula (I), wherein X is OCH2 or CH2, and Y is H, halogen or CH2=CH2CH2. cTDPRE of the invention is a cADPR analogue with membrane permeability, which has the advantages of simple structure, convenient synthesis, high activity and the like. The product has good stability, so the product can be used as a pharmacological tool molecule for studying calcium release of a cADPR mediated intracellular calcium store and intracellular calcium signal transduction which is participated by the cADPR. The method for preparing intracellular focus phosphate by microwave irradiation in the invention can avoid the super sparse solution condition in the existing method for synthesizing the intracellular focus phosphate, simplify synthesis operation, and is convenient for realizing large-scale preparation of the cADPR analogue ...

Method for removing iron and enriching nickel cobalt through precipitation of laterite type nickel ores

The invention relates to a method for removing iron and enriching nickel cobalt through precipitation of laterite type nickel ores, which is characterized in that: performing ball milling on the laterite type nickel ores, sieving the laterite type nickel ores through 50 mesh sieves, sampling a 50 mesh ore sample, using a hydrochloric acid to leach out the 50 mesh ore sample, driving the Fe concentration in lixivium to between 0.01 and 6 mol/L, and adding an oxidant and a precipitator into a solution, wherein the concentration of the oxidant and the precipitator is between 0.01 and 9 mol/L; using 0.01 to 6 mol/L of aqueous alkali to control the pH value of the system to between 0.1 and 6.0, performing reaction on the system in a stirring reactor at the temperature of between 20 and 90 DEG C for 1 minute to 24 hours, obtaining deposit after solid-liquid separation, regenerating the hydrochloric acid during iron removal through precipitation, returning the regenerated hydrochloric acid to ...

Method for the preparation of high rate lithium ion capacitor battery cathode material by pyrolyzing asphalt at low temperature to cover graphite

The invention relates to a method for the preparation of high rate lithium ion capacitor battery cathode material by pyrolyzing asphalt at low temperature to cover graphite, belonging to the technical field of the preparation of energy storage material. The invention comprises the steps that: first, mixed solution containing graphitizing catalyst, water and alcohol is formulated, then graphite, asphalt powder and conductive agent are added therein for being mixed for several hours, the resultant mixture is dried in an atmosphere of the air, the asphalt is then pyrolyzed in an atmosphere of Aror N2, and in the end, salpeter solution is used to remove the graphitizing catalyst by means of dissolution, and the high rate lithium ion capacitor battery cathode material with pyrolyzed asphalt covering the graphite is obtained by sufficient washing and drying. The cathode material obtained according to the method has the characteristics of high capacity and good high rate performance, is simple ...

Method for preparing paris polyphylla total saponin

Method for preparing ferric lithium phosphate precursor by comprehensive utilization of ilmenite

The invention discloses a preparation method for lithium iron phosphate precursor by comprehensively utilizing ilmenite, comprising a plurality of steps: leach the ilmenite in acid and filtrate is obtained by filtering; dissolve a certain amount of other iron sources in the filtrate to make the Fe concentration in the mixed solution as 0.01-3mol/L and the molar ratio of Ti and Fe as 0.0005-0.5; add appropriate amount of oxidant to the mixed solution; use alkali aqueous solution to regulate the system pH as 4.0-14.0; react for 10min to 24h at 10-90 DEG Cin a stir reactor; filter, wash and dry the sedimentation at 50-159 DEG C; after drying, calcine the sedimentation in air at 300-800 DEG C for 1-24h; lithium iron phosphate precursor for the lithium-ion battery cathode material, which is also the ferric oxide of doped-metal elements, can be obtained. The preparation method has the advantages of wide material source, simple technological process, good and stable product quality and low cost ...

Method for producing battery-grade ferrous oxalate by using ferrous sulfate as titanium pigment byproduct

The invention discloses a method for producing battery-grade ferrous oxalate by using ferrous sulfate as a titanium pigment byproduct. The waste and byproduct ferrous sulfate is dissolved in deionized water; the mixture is filtered; a complexing agent, a surfactant and a precipitation agent are added according to a certain order into the filtrate when violent stirring is carried out; then, aqueous solution of alkaline or acid is used for controlling the pH of the system to be 0.1-5.0, the stirring is not stopped until reaction is carried out at temperature of 20-80 DEG C for 5 min-4 h, aging is carried out for 0-24 h; the obtained sediment is filtered, washed and dried so as to obtain the battery-grade ferrous oxalate, wherein the iron recovery rate can be as high as 99.9 percent. By adjusting the addition order and the addition amount of the additives as well as the pH value of the system, the method causes the types and the amount of metallic elements in the ferrous oxalate to be controllable ...

Dust suction apparatus

The invention provides a dust suction device which can satisfactorily remove sundries such as flocky and dust from the gap of an optical device, dispensed with dismantling the optical device. The front end (20b) of a hose nozzle is provided with a nozzle (30) which can be deeply inserted into the gap of the optical device; the nozzle (30) is made of elastic material and is formed in a shape of gradual bending. In addition, the frontal end (30a) of the nozzle (30) is provided with a frontal suction mouth (31). The frontal suction mouth (31) is formed by slantways incising a side wall (31a) that forms the frontal end (30a) in a cross-cutting manner.

Method for improving nickel cobalt leaching rate from lateritic nickel at low cost

The invention provides a low-cost method for improving the nickel-cobalt leaching rate of low-grade lateritic nickel ore. In the method, laterite ore is roasted twice, wherein primary roasting is hermetically carried out for about 30 minutes at a temperature between 90 and 110 DEG C, and secondary roasting is carried out for about 1 hour at a temperature between 260 and 420 DEG C under the condition with air supply, so as to change the phase structure of nickel-cobalt-containing metal in mineral, ensure that the nickel-cobalt-containing metal is easier to leach by leaching agents and raise the nickel-cobalt leaching rate with low acid consumption at a low temperature. At the same time of roasting, the method changes the existence structure of iron, increases leaching activation energy, and reduces the leaching of iron. A roasting material is cooled to about 50 DEG C in air, and leaching is carried out by adding sulfuric acid or hydrochloric acid at about 50 DEG C. The leaching rate of nickel ...

Production method of projector

The invention provides a manufacturing method of a projector. The method can reduce the working procedure quantity of dustproof operation, eliminates the complexity of operation and improves the technical performance. A cooling device forming working procedure is used for assembling a Xiluoke fan (21) and an air inlet side pipeline (30) (an air inlet pipeline (31) and a blast pipeline (35) to form a cooling device (20). A cooling device assembling working procedure is used for assembling the cooling device (20) at a lower casing (15). A power supply device connecting working procedure is used for connecting a power supply device (40) with the Xiluoke fan (21). A dust removing working procedure is used for forcing the Xiluoke fan (21) to rotate and produce attracting wind, which ensures that the sundries of batting, dust etc. inside the air inlet pipeline (31), the Xiluoke fan (21) and the blast pipeline (35) are carried in the attracting wind and are removed to the outside through blowing ...

Method for increasing yield of prodn. of hypericum japonicum total flavone

Metal resin anchoring rod body automatic thread-rolling conveyor

The invention discloses a metal resin anchoring rod body automatic thread-rolling conveyor, which belongs to the automatic thread-rolling conveyor. A rolling-over feeding mechanism, a chain conveying mechanism, a pneumatic leveling mechanism, a thread-rolling pushing mechanism and a rod throwing mechanism of the automatic thread-rolling conveyor are orderly connected to a frame; sensors are connected to the rolling-over feeding mechanism, the chain conveying mechanism, the pneumatic leveling mechanism, the thread-rolling pushing mechanism and the rod throwing mechanism respectively and are all connected with a PLC; the output end of an air compressor is connected with the rolling-over feeding mechanism, the pneumatic leveling mechanism, the thread-rolling pushing mechanism and the rod throwing mechanism; and a motor is connected with the chain conveying mechanism. The automatic thread-rolling conveyor has the advantages of improving machining accuracy and consistency, improving labor productivity ...

Data acquisition device for vector spectrum analysis on vibration signals

The invention relates to a data acquisition device for vector spectrum analysis on vibration signals. The data acquisition device comprises an embedded CPU (Central Processing Unit), wherein a sensor interface circuit is connected with and arranged on the embedded CPU and provided with at least one sensor input interface; the output of the sensor interface circuit is connected with an amplifying and filtering circuit used for processing AC signals and an amplifying circuit used for processing DC signals through an AC/DC separation circuit respectively; output terminals of both the amplifying and filtering circuit and the amplifying circuit are connected with an A/D conversion module; the A/D conversion module is connected with a corresponding port of the embedded CPU; and a constant-current source module controlled by the embedded CPU and used for supplying power for a sensor corresponding to a sensor interface module is also arranged on an output port of the embedded CPU. The acquisition ...

Novel Isobaric Tandem Mass Tags for Quantitative Proteomics and Peptidomics

Compositions and methods of tagging peptides and other molecules using novel isobaric tandem mass tagging reagents, including novel N,N-dimethylated amino acid 8-plex and 16-plex isobaric tandem mass tagging reagents. The tagging reagents comprise: a) a reporter group having at least one atom that is optionally isotopically labeled; b) a balancing group, also having at least one atom that is optionally isotopically labeled, and c) an amine reactive group. The tagging reagents disclosed herein serve as attractive alternatives for isobaric tag for relative and absolute quantitation (iTRAQ) and tandem mass tags (TMTs) due to their synthetic simplicity, labeling efficiency and improved fragmentation efficiency. 2. The method of further comprising identifying one or more fragments of the labeled molecule using mass spectrometry.3. The method of further comprising quantifying amounts of said one or more fragments of the labeled molecule.5. The method of wherein Ris selected from the group consisting of:{'sup': 12', '13, 'a) a methyl group containing one or more deuterium atoms and wherein the carbon is C or C;'}b) hydrogen;c) deuterium;{'sup': '13', 'd) an isopropyl group containing one or more deuterium atoms and one or more C atoms; and'}{'sup': '13', 'e) a butyl group containing one or more deuterium atoms and one or more C atoms.'}6. The method of wherein said molecule is a peptide claim 1 , metabolite or neurotransmitter.7. The method of where labeling the peptide comprises the step of reacting the amine reactive group of the tagging reagent with the N-terminus of the peptide or with an ε-amino group within peptide.8. A method of analyzing a mixture containing target molecules comprising the steps of:a) labeling target molecules within a first sample with a first tagging reagent, thereby generating first labeled target molecules;b) labeling target molecules within at least one additional sample with at least one additional tagging reagent, thereby generating ...

METHOD FOR THE SITE-SPECIFIC ENZYMATIC LABELLING OF NUCLEIC ACIDS IN VITRO BY INCORPORATION OF UNNATURAL NUCLEOTIDES

Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker. 2. The double-stranded oligonucleotide of claim 1 , wherein the first compound and the second compound form a base pair in the double-stranded oligonucleotide.3. The double-stranded oligonucleotide of claim 2 , wherein the Ris hydrogen or halogen.4. The double-stranded oligonucleotide of claim 3 , wherein the Ris hydrogen.5. The double-stranded oligonucleotide of claim 3 , wherein the Ris halogen.6. The double-stranded oligonucleotide of claim 5 , wherein the halogen is a fluoro.14. The composition of claim 13 , wherein the first compound and the second compound form a base pair.15. The composition of claim 14 , wherein the Ris hydrogen or halogen.16. The composition of claim 15 , wherein the Ris hydrogen.24. The composition of claim 23 , wherein the first compound and the second compound form a base pair.25. The composition of claim 24 , wherein the Ris hydrogen. This is a continuation of U.S. patent application Ser. No. 16/518,715, filed on Jul. 22, 2019, which is a continuation of U.S. patent application Ser. No. 14/910,203, filed Feb. 4, 2016; which is a U.S. National Stage Entry of PCT/US2014/050423, filed Aug. 8, 2014; which claims the benefit of priority to U.S. Provisional Patent Application No. 61/863,649, filed Aug. 8, 2013; all of which are herein incorporated by reference in their entirety.This ...

MULTI-FACTOR AUTHENTICATION AND ACCESS CONTROL IN A VEHICULAR ENVIRONMENT

The systems and methods described herein can include a digital assistant application that receives sensor signals from sensors installed in a vehicle and determines an entry event into the vehicle. The digital assistant application can receive, responsive to the entry event into the vehicle, a plurality authentication input signals from a plurality of sensors associated with the vehicle. The digital assistant application can determine a plurality of authentication states based on the plurality of authentication input signals and a plurality of authentication credentials. The digital assistant application can identify an access permission level of a plurality of access permission levels based at least in part on the plurality of identifies authentication states. The digital assistant application can identify, responsive to the access permission level, a subset of a set of functionalities available via the vehicle, and provide vehicular access to the subset of functionalities. 1. A method implemented by one or more processors , comprising:receiving a first authentication input signal, the first authentication input signal including first sensor data obtained by one or more first sensors associated with a vehicle or a mobile device of a user;determining a first authentication state based on processing the first sensor data obtained by one or more of the first sensors associated with the vehicle or the mobile device of the user, wherein determining the first authentication state comprises performing one of: a key fob authentication, a mobile device authentication, a speaker recognition authentication, or a face recognition authentication;receiving a second authentication input signal, the second authentication input signal including second sensor data obtained by one or more second sensors associated with the vehicle or the mobile device of the user;determining a second authentication state based on processing the second sensor data obtained by one or more of the second ...

A METHOD FOR THE SITE-SPECIFIC ENZYMATIC LABELLING OF NUCLEIC ACIDS IN VITRO BY INCORPORATION OF UNNATURAL NUCLEOTIDES

Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker. 120.-. (canceled)22. The method of claim 21 , wherein the method is performed in a cell.23. The method of claim 22 , wherein the cell is a prokaryotic cell.24. The method of claim 21 , wherein Ris hydrogen or halogen.25. The method of claim 24 , wherein the halogen is fluoro.26. The method of claim 24 , wherein Ris hydrogen.27. The method of claim 21 , further comprising retaining the unnatural base pair in the DNA oligonucleotide after two or more rounds of replication.28. The method of claim 27 , wherein retaining is at a rate of at least 90% claim 27 , as measured by polymerase chain reaction (PCR) under standard conditions.29. The method of claim 28 , wherein retaining is at a rate of at least 99% claim 28 , as measured by polymerase chain reaction (PCR) under standard conditions.33. The method of claim 32 , wherein the method is performed in a cell.34. The method of claim 33 , wherein the RNA polymerase is endogenous to the cell.35. The method of claim 33 , wherein the cell is a prokaryotic cell.36. The method of claim 32 , wherein Ris hydrogen or halogen.37. The method of claim 36 , wherein the halogen is fluoro.38. The method of claim 36 , wherein Ris hydrogen. This is a continuation of U.S. patent application Ser. No. 14/910,203, filed Feb. 4, 2016; which is a U.S. National Stage Entry of PCT/US2014/050423 ...

SYSTEMS AND METHODS FOR AUTHENTICATING A USER THROUGH AN UNOBSERVABLE RE-AUTHENTICATION SYSTEM

Systems and methods for authenticating a user through an unobservable re-authentication process are disclosed. 1. A method to authenticate an user of a device through an unobservable authentication process , the method comprising:collecting a plurality of data related to an authorized owner;capturing a plurality of data related to a user's interaction with a device; andauthenticating the user based on the user's interaction with the device through an authentication module to determine if the user is the authorized owner, wherein authenticating the user is automatic and does not require specific prompts to the user.2. The method of claim 1 , wherein the plurality of data related to an authorized owner may be collected from a training module or from the authorized owner's interaction with the device claim 1 , the training module further comprising:initializing the authorized owner's gesture feature data;obtaining a plurality of classification modules from the authorized owner's gesture feature data;downloading a plurality of other people's gesture feature data to the device wherein the other people's gesture feature data may be anonymous and/or used for training purposes;uploading the authorized owner's gesture feature data to a sever; anddownloading the gesture feature data from the server onto the device.3. The method of claim 1 , wherein the authentication module further comprises:assembling the plurality of user interaction data into a plurality of gestures;extracting a plurality of features from the plurality of gestures; andpredicting whether the plurality of features belong to the authorized owner.4. The method of claim 1 , wherein the user interaction data may comprise:directional swiping motions; andtapping motions.5. The method of claim 2 , wherein the classification module of the training module is determined based on a plurality of factors claim 2 , comprising:the user's gesture features data; anda plurality of features from a plurality of other users.6. ...

MULTI-FACTOR AUTHENTICATION AND ACCESS CONTROL IN A VEHICULAR ENVIRONMENT

The systems and methods described herein can include a digital assistant application that receives sensor signals from sensors installed in a vehicle and determines an entry event into the vehicle. The digital assistant application can receive, responsive to the entry event into the vehicle, a plurality authentication input signals from a plurality of sensors associated with the vehicle. The digital assistant application can determine a plurality of authentication states based on the plurality of authentication input signals and a plurality of authentication credentials. The digital assistant application can identify an access permission level of a plurality of access permission levels based at least in part on the plurality of identifies authentication states. The digital assistant application can identify, responsive to the access permission level, a subset of a set of functionalities available via the vehicle, and provide vehicular access to the subset of functionalities. 1. A system for multi-factor entry authentication of vehicle entry events , comprising:a data processing system having a digital assistant application that includes an entry detection component, an entry authentication component, and an access control component, the digital assistant application executes in a vehicle to:receive, by the an entry detection component, at least one sensor signal obtained by a first sensor installed in the vehicle;determine, by the entry detection component, based on the at least one sensor signal, an entry event into the vehicle, the entry event indicative of a user entering the vehicle;receive, by the entry authentication component, responsive to the entry event into the vehicle, a first authentication input signal and a second authentication input signal, the first authentication input signal and the second authentication signal obtained by at least one second sensor associated with the vehicle;determine, by the entry authentication component, a first ...

Development of a Novel Mass Spectrometry-Based Method for Simultaneous Qualitative and Quantitative Protein Citrullination Analysis of Complex Biological Samples

The present invention discloses mass spectrometry (MS)-based methods and tagging reagents for qualitative and quantitative analysis of post-translational modified (PTM) proteins in biological and clinical samples. The present invention utilizes thiol-containing tagging reagents which are able to bind to or derivatize biomolecules, preferably post-translational modified (PTM) polypeptides such as citrullinated and homocitrullinated polypeptides. The tagging reagents are preferably biotin-derived tags able to react with a side chain of the modified polypeptides.

A METHOD FOR THE SITE-SPECIFIC ENZYMATIC LABELLING OF NUCLEIC ACIDS IN VITRO BY INCORPORATION OF UNNATURAL NUCLEOTIDES

Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker. 3. The compound of claim 1 , wherein the reactive center comprises an amino group claim 1 , an acetylenic group claim 1 , a thiol group claim 1 , an aldehyde group claim 1 , or an azide group.4. The compound of claim 2 , wherein the reactive center comprises an amino group claim 2 , an acetylenic group claim 2 , a thiol group claim 2 , an aldehyde group claim 2 , or an azide group.5. The compound of claim 1 , wherein the ribosyl or deoxyribosyl moiety bears a triphosphate or an α-thiotriphosphate group bonded to a 5′-hydroxyl thereof.6. The compound of claim 2 , wherein the ribosyl or deoxyribosyl moiety bears a triphosphate or an α-thiotriphosphate group bonded to a 5′-hydroxyl thereof.7. The compound of claim 1 , wherein the ribosyl or deoxyribosyl moiety is incorporated into a RNA or DNA oligonucleotide chain claim 1 , respectively claim 1 , or the ribosyl or deoxyribosyl moiety or analog thereof is incorporated into an RNA or a DNA analog.8. The compound of claim 2 , wherein the ribosyl or deoxyribosyl moiety is incorporated into a RNA or DNA oligonucleotide chain claim 2 , respectively claim 2 , or the ribosyl or deoxyribosyl moiety or analog thereof is incorporated into an RNA or a DNA analog.9. The compound of claim 1 , wherein at least one Rindependently comprises a group selected from the group consisting ...

Isobaric Multiplex Reagents for Carbonyl Containing Compound High-Throughput Quantitative Analysis

The present invention provides a set of novel isobaric chemical tags, also referred herein as SUGAR (Isobaric Multiplex Reagents for Carbonyl Containing Compound). These labeling tags are compact and easy to synthesize at high yield and purity in just a few steps using commercially available starting materials. The tagging reagents of the present invention comprise: a) a reporter group, having at least one atom that is optionally isotopically labeled; b) a balancing group, also having at least one atom that is optionally isotopically labeled, and c) an aldehyde, ketone, or carboxylic acid reactive group. The multiplex SUGAR tags are able to react with an aldehyde, ketone, or carboxylic acid group of the molecule to be tagged, which offers the capability for labeling and quantitation of glycans, proteins/peptides, and fatty acids. 2. The method of wherein Ris a hydrazide claim 1 , hydrazine claim 1 , amine claim 1 , or oxyamine.3. The method of wherein Ris a hydrazide.4. The method of wherein Rand R claim 1 , independently of one another claim 1 , are CH claim 1 , CH claim 1 , CDH claim 1 , CDH claim 1 , CDH claim 1 , CDH claim 1 , CDor CD.5. The method of wherein at least one of Ror Rcontains a deuterium atom claim 1 , and Nis N claim 1 , or Nis N.6. The method of wherein Ris hydrogen or deuterium.7. The method of wherein Ris hydrogen or deuterium and Ris selected from the group consisting of:{'sup': 12', '13, 'a) a methyl group containing one or more deuterium atoms and wherein the carbon is C or C;'}b) hydrogen;c) deuterium;{'sup': '13', 'd) an isopropyl group containing one or more deuterium atoms and one or more C atoms; and'}{'sup': '13', 'e) a butyl group containing one or more deuterium atoms and one or more C atoms.'}8. The method of wherein the labeling step comprises reacting the tagging reagent with a carboxylic acid group of the target molecule.9. The method of wherein the step of labeling the target molecule with a tagging reagent has a labeling ...

Mass Defect-Based Multiplex Dimethyl Pyrimidinyl Ornithine (DiPyrO) Tags for High-Throughput Quantitative Proteomics and Peptidomics

The use of mass defect signatures to impart milliDalton mass differences between isotopically labeled peptides at the MS 1 -level allows multiplex quantification without the increased mass spectral complexity that occurs with mass difference approaches. Provided herein is a mass defect-based chemical tag, dimethyl pyrimidinyl ornithine (DiPyrO), that is compact and easy to synthesize at high purity in few steps using commercially available starting materials. The multiplex DiPyrO tags are amine-reactive and can impart a mass difference onto labeled peptides and through calculated substitution of heavy isotopes. DiPyrO offers up to 10-plex quantification on current Orbitrap or FT-ICR platforms without increasing mass spectral complexity. The synthesis of the DiPyrO tag is provided along with viability of the DiPyrO tag for labeling complex proteomics samples using yeast extract digests and its effect on labeled peptides during LC-MS 2 analysis. Labeling and quantification of glycans and metabolites using the DiPyrO tag is also demonstrated.

Novel Isobaric Tandem Mass Tags for Quantitative Proteomics and Peptidomics

Compositions and methods of tagging peptides and other molecules using novel isobaric tandem mass tagging reagents, including novel N, N-dimethylated amino acid 8-plex and 16-plex isobaric tandem mass tagging reagents. The tagging reagents comprise: a) a reporter group having at least one atom that is optionally isotopically labeled; b) a balancing group, also having at least one atom that is optionally isotopically labeled, and c) an amine reactive group. The tagging reagents disclosed herein serve as attractive alternatives for isobaric tag for relative and absolute quantitation (iTRAQ) and tandem mass tags (TMTs) due to their synthetic simplicity, labeling efficiency and improved fragmentation efficiency. 131-. (canceled)32. A method of analyzing a mixture containing target molecules comprising the steps of: {'br': None, 'sub': 3', '2, 'sup': 1', '2', '1, '(CH)-AA-AA-R\u2003\u2003(formula 16)'}, 'a) labeling target molecules within a first sample with a first tagging reagent, thereby generating first labeled target molecules, wherein the first tagging reagent has the formula{'sup': '1', 'where Ris an amine reactive group;'}{'sup': '1', 'AAis a first amino acid having an N-terminus;'}{'sup': '2', 'AAis a second amino acid having an N-terminus; and'}{'sub': '3', 'sup': '1', 'the two CHgroups are attached to the N-terminus of AA;'} {'br': None, 'sub': 3', '2, 'sup': 2', '1', '1, '(CH)-AA-AA-R\u2003\u2003(formula 17)'}, 'b) labeling target molecules within one or more additional samples with one or more additional tagging reagents, thereby generating additional labeled target molecules, wherein at least one of the additional tagging reagents has the formula{'sup': 1', '2, 'where AAand AAare the same amino acids as in the first tagging reagent with the exception that the amino acids may contain different isotopes and'}{'sub': '3', 'sup': '2', 'the two CHgroups are attached to the N-terminus of AA;'}c) combining the labeled target molecules of steps a) and b);d) ...

THIENOPYRIDINE DERIVATIVES CONTAINING UNSATURATED ALIPHATIC OLEFINIC BOND, PREPARATION METHOD AND USE THEREOF

The present invention provides a compound having a structure of formula (I), a preparation method and use thereof, and a pharmaceutical composition containing the compound, wherein R is methyl, ethyl, propyl, vinyl or propenyl. The present invention also provides a crystalline form of the compound, a preparation method and use of the crystalline form, and a pharmaceutical composition comprising the crystalline form. The compounds having the structure of formula (I) of the present invention are present in a solid form, which not only can solve the problem of clopidogrel resistance, but also can solve the problem of severe hemorrhagic side effect and poor safety of some drugs, as well as the problem of poor stability of existing compounds. It can be developed into an ADP receptor antagonist antiplatelet agent with clear therapeutic effect, no resistance and better stability. 2. The compound according to claim 1 , which is selected from the following compounds:I-1: methyl (S,E)-2-(2-chlorophenyl)-2-(2-(2-butenoyloxy)-6,7-dihydrothieno[3,2-c]pyridin-5(4H)-yl)-acetate;I-2: methyl (S)-2-(2-chlorophenyl)-2-(2-((2E,4E)-2,4-hexadienoyloxy)-6,7-dihydrothieno[3,2-c]pyridin-5(4H)-yl)-acetate;I-3: methyl (S,E)-2-(2-chlorophenyl)-2-(2-(2,4-pentadienoyloxy)-6,7-dihydrothieno[3,2-c]pyridin-5(4H)-yl)-acetate;I-4: methyl (S,E)-2-(2-chlorophenyl)-2-(2-(2-pentenoyloxy)-6,7-dihydrothieno[3,2-c]pyridin-5(4H)-yl)-acetate; andI-5: methyl (S,E)-2-(2-chlorophenyl)-2-(2-(2-hexenoyloxy)-6,7-dihydrothieno[3,2-c]pyridin-5(4H)-yl)-acetate.5. Use of the compound having the structure of formula (I) according to or in the manufacture of a medicament for preventing and/or treating cardiovascular and cerebrovascular diseases caused by platelet aggregation.6. A pharmaceutical composition comprising the compound having the structure of formula (I) according to or and a pharmaceutically acceptable carrier or excipient.7. The pharmaceutical composition according to claim 6 , wherein said pharmaceutical ...

REAGENTS AND METHODS FOR REPLICATION, TRANSCRIPTION, AND TRANSLATION IN SEMI-SYNTHETIC ORGANISMS

Disclosed herein are compositions, methods, cells, engineered microorganisms, and kits for increasing the production of proteins or polypeptides comprising one or more unnatural amino acids. Further provided are compositions, cells, engineered microorganisms, and kits for increasing the retention of unnatural nucleic acids encoding the unnatural amino acids in an engineered cell, or semi-synthetic organism. 2. The nucleobase of claim 1 , wherein X is carbon claim 1 , and/or E is sulfur and/or Y is sulfur.3. (canceled)4. (canceled)6. (canceled)8. A double stranded oligonucleotide duplex wherein a first oligonucleotide strand comprises the nucleobase of claim 1 , and a second complementary oligonucleotide strand comprises a complementary base-pairing nucleobase in a complementary base-pairing site thereof.10. (canceled)11. (canceled)13. (canceled)14. An mRNA or tRNA encoded by the plasmid of claim 12 , encoding the protein.15. A transfer RNA (tRNA) comprising the nucleobase of claim 1 , comprising:an anticodon, wherein the anticodon comprises the nucleobase, optionally wherein the nucleobase is at the first, second, or third position of the anticodon; anda recognition element, wherein the recognition element promotes selective charging of the tRNA with an unnatural amino acid by an aminoacyl tRNA synthetase.16. (canceled)17. (canceled)18. (canceled)20. (canceled)21. A polynucleotide library claim 1 , wherein the library comprises at least 5000 unique polynucleotides claim 1 , and wherein each polynucleotide comprises at least one nucleobase of .23. (canceled)24. (canceled)26. (canceled)27. A method of transcribing a DNA to a tRNA or an mRNA encoding a protein comprising:contacting a DNA comprising a gene encoding the tRNA or protein with ribonucleoside triphosphates and an RNA polymerase, wherein the gene encoding the tRNA or protein comprises a first unnatural base paired to and forming a first unnatural base pair with a second unnatural base, and wherein the ...

Isobaric tandem mass tags for quantitative proteomics and peptidomics

Compositions and methods of tagging peptides and other molecules using novel isobaric tandem mass tagging reagents, including novel N, N-dimethylated amino acid 8-plex and 16-plex isobaric tandem mass tagging reagents. The tagging reagents comprise: a) a reporter group having at least one atom that is optionally isotopically labeled; b) a balancing group, also having at least one atom that is optionally isotopically labeled, and c) an amine reactive group. The tagging reagents disclosed herein serve as attractive alternatives for isobaric tag for relative and absolute quantitation (iTRAQ) and tandem mass tags (TMTs) due to their synthetic simplicity, labeling efficiency and improved fragmentation efficiency.

Multi-factor authentication and access control in vehicular environment

【課題】乗物に設置されたセンサからセンサ信号を受け取って乗物へのエントリイベントを判定するデジタルアシスタントアプリケーションを提供する。【解決手段】デジタルアシスタントアプリケーションは、乗物へのエントリイベントに応答して、乗物に関連した複数のセンサから複数の認証入力信号を受け取ることができる。デジタルアシスタントアプリケーションは、複数の認証入力信号および複数の認証情報に基づいて複数の認証状態を判定することができる。デジタルアシスタントアプリケーションは、複数の識別認証状態に少なくとも部分的に基づき、複数のアクセス許可レベルのうちの1つのアクセス許可レベルを識別することができる。デジタルアシスタントアプリケーションは、アクセス許可レベルに応答して、乗物を介して利用可能な機能のセットのサブセットを識別し、機能のサブセットへの乗物アクセスを提供することができる。【選択図】図３

A method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides

Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase- mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo -bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.

A method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides

Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.

Multi-factor authentication and access control in a vehicular environment

The systems and methods described herein can include a digital assistant application that receives sensor signals from sensors installed in a vehicle and determines an entry event into the vehicle. The digital assistant application can receive, responsive to the entry event into the vehicle, a plurality authentication input signals from a plurality of sensors associated with the vehicle. The digital assistant application can determine a plurality of authentication states based on the plurality of authentication input signals and a plurality of authentication credentials. The digital assistant application can identify an access permission level of a plurality of access permission levels based at least in part on the plurality of identifies authentication states. The digital assistant application can identify, responsive to the access permission level, a subset of a set of functionalities available via the vehicle, and provide vehicular access to the subset of functionalities

Reagents and methods for replication, transcription, and translation in semi-synthetic organisms

Disclosed herein are compositions, methods, cells, engineered microorganisms, and kits for increasing the production of proteins or polypeptides comprising one or more unnatural amino acids. Further provided are compositions, cells, engineered microorganisms, and kits for increasing the retention of unnatural nucleic acids encoding the unnatural amino acids in an engineered cell, or semi-synthetic organism.

Multi-factor authentication and access control in a vehicular environment

The systems and methods described herein can include a digital assistant application that receives sensor signals from sensors installed in a vehicle and determines an entry event into the vehicle. The digital assistant application can receive, responsive to the entry event into the vehicle, a plurality authentication input signals from a plurality of sensors associated with the vehicle. The digital assistant application can determine a plurality of authentication states based on the plurality of authentication input signals and a plurality of authentication credentials. The digital assistant application can identify an access permission level of a plurality of access permission levels based at least in part on the plurality of identifies authentication states. The digital assistant application can identify, responsive to the access permission level, a subset of a set of functionalities available via the vehicle, and provide vehicular access to the subset of functionalities.

Lens

Thienopyridine derivatives containing an ethylenic ester, preparation method therefor and use thereof

Mass defect-based multiplex dimethyl pyrimidinyl ornithine (dipyro) tags for high-throughput quantitative proteomics and peptidomics

The use of mass defect signatures to impart milliDalton mass differences between isotopically labeled peptides at the MS1-level allows multiplex quantification without the increased mass spectral complexity that occurs with mass difference approaches. Provided herein is a mass defect-based chemical tag, dimethyl pyrimidinyl ornithine (DiPyrO), that is compact and easy to synthesize at high purity in few steps using commercially available starting materials. The multiplex DiPyrO tags are amine-reactive and can impart a mass difference onto labeled peptides and through calculated substitution of heavy isotopes. DiPyrO offers up to 10-plex quantification on current Orbitrap or FT-ICR platforms without increasing mass spectral complexity. The synthesis of the DiPyrO tag is provided along with viability of the DiPyrO tag for labeling complex proteomics samples using yeast extract digests and its effect on labeled peptides during LC-MS2 analysis. Labeling and quantification of glycans and metabolites using the DiPyrO tag is also demonstrated.

Mass defect-based multiplex dimethyl pyrimidinyl ornithine (dipyro) tags for high-throughput quantitative proteomics and peptidomics

Mass defect-based multiplex dimethyl pyrimidinyl ornithine (dipyro) tags for high-throughput quantitative proteomics and peptidomics

The use of mass defect signatures to impart milliDalton mass differences between isotopically labeled peptides at the MS 1 -level allows multiplex quantification without the increased mass spectral complexity that occurs with mass difference approaches. Provided herein is a mass defect-based chemical tag, dimethyl pyrimidinyl ornithine (DiPyrO), that is compact and easy to synthesize at high purity in few steps using commercially available starting materials. The multiplex DiPyrO tags are amine-reactive and can impart a mass difference onto labeled peptides and through calculated substitution of heavy isotopes. DiPyrO offers up to 10-plex quantification on current Orbitrap or FT-ICR platforms without increasing mass spectral complexity. The synthesis of the DiPyrO tag is provided along with viability of the DiPyrO tag for labeling complex proteomics samples using yeast extract digests and its effect on labeled peptides during LC-MS 2 analysis. Labeling and quantification of glycans and metabolites using the DiPyrO tag is also demonstrated.

Multiplexed DiLeu-Biotin-Azide (DBA) Tag Enabled Isobaric Tandem Orthogonal Proteolysis Activity-Based Protein Profiling (isoBOP-ABPP) Platform For High-Throughput Quantitative Pan-PTM Analysis

The present invention provides improved cleavable biotin-containing isobaric tags for quantitative mass spectrometry proteomics, particularly useful in quantification of post-translational modifications (PTMs) and assessing cysteine containing proteins. The isobaric chemical tags, DiLeu-Biotin-Azide (DBA), consist of three “modules”: (1) a DiLeu reporter group for relative quantification using MS/MS; (2) a biotin moiety for selective enrichment via streptavidin beads; and (3) an azide functional group to enable biorthogonal click chemistry. The DBA tags could be used in a high-throughput quantitative pan-PTM analysis platform.

Multi-factor authentication and access control in a vehicular environment

The systems and methods described herein can include a digital assistant application that receives sensor signals from sensors installed in a vehicle and determines an entry event into the vehicle. The digital assistant application can receive, responsive to the entry event into the vehicle, a plurality authentication input signals from a plurality of sensors associated with the vehicle. The digital assistant application can determine a plurality of authentication states based on the plurality of authentication input signals and a plurality of authentication credentials. The digital assistant application can identify an access permission level of a plurality of access permission levels based at least in part on the plurality of identifies authentication states. The digital assistant application can identify, responsive to the access permission level, a subset of a set of functionalities available via the vehicle, and provide vehicular access to the subset of functionalities.

Reagents and methods for replication, transcription, and translation in semi-synthetic organisms

Disclosed herein are compositions, methods, cells, engineered microorganisms, and kits for increasing the production of proteins or polypeptides comprising one or more unnatural amino acids. Further provided are compositions, cells, engineered microorganisms, and kits for increasing the retention of unnatural nucleic acids encoding the unnatural amino acids in an engineered cell, or semi-synthetic organism.

Method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides

Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.

非天然ヌクレオチドの取り込みによるインビトロの核酸の部位特異的な酵素標識のための方法

【課題】ＤＮＡポリメラーゼを媒介としたＤＮＡの複製とＲＮＡポリメラーゼを媒介としたＲＮＡの転写の間に非天然の塩基対を形成する疎水性の核酸塩基アナログを優勢的に有する非天然のヌクレオチドのアナログを提供する。【解決手段】式β９ｂを有する第１の核酸塩基アナログと、式α１５ａまたはα１５ｂのいずれかを有する第２の核酸塩基アナログを含む核酸塩基対。JPEG2023179406000042.jpg35126[式中、ＸはＣ又はN；Ｒ１、Ｒ２は特定のリンカー；Ｙは硫黄、セレン、または二級アミン；Ｅは酸素、硫黄またはセレン；波線は、リボシル、デオキシリボシル、あるいはジデオキシリボシル部分またはそのアナログとの結合点を示す]【選択図】図１

Derivados de tienopiridina que contienen un enlace olefínico alifático insaturado, método de preparación y uso de los mismos

Se proporcionan un compuesto que tiene la estructura de fórmula (I), un método de preparación del mismo y su uso, y una composición farmacéutica que comprende el compuesto, en la que R es metilo, etilo, propilo, vinilo o propenilo. También se proporcionan una forma cristalina del compuesto, un método de preparación y uso de la forma cristalina, y una composición farmacéutica que comprende la forma cristalina. El compuesto que tiene la estructura de fórmula (I) de la presente invención existe en forma sólida, en la que no sólo puede resolver el problema de la resistencia al clopidogrel, sino que también puede resolver los problemas de los efectos secundarios de grandes cantidades de sangrado y la mala seguridad de ciertos medicamentos y también puede resolver el problema de la mala estabilidad de los compuestos existentes y, por lo tanto, el compuesto puede convertirse en un fármaco antiplaquetario antagonista del receptor de ADP con clara eficacia, sin resistencia y mejor estabilidad. (Traducción automática con Google Translate, sin valor legal)

Tricyclic boronic acid derivative, and preparation method therefor and application thereof

Disclosed are a tricyclic boronic acid derivative, and a preparation method therefor and an application thereof. Specifically, discloses are a compound represented by formula (l), an optical isomer and pharmaceutically acceptable salt thereof, and an application of the compound as a 13-lactamase inhibitor and an application of a composition as an antibacterial drug.

Carbonyl containing compound high-throughput quantitative analysis using isobaric multiplex reagents

The present invention provides a set of novel isobaric chemical tags, also referred herein as SUGAR (Isobaric Multiplex Reagents for Carbonyl Containing Compound). These labeling tags are compact and easy to synthesize at high yield and purity in just a few steps using commercially available starting materials. The tagging reagents of the present invention comprise: a) a reporter group, having at least one atom that is optionally isotopically labeled; b) a balancing group, also having at least one atom that is optionally isotopically labeled, and c) an aldehyde, ketone, or carboxylic acid reactive group. The multiplex SUGAR tags are able to react with an aldehyde, ketone, or carboxylic acid group of the molecule to be tagged, which offers the capability for labeling and quantitation of glycans, proteins/peptides, and fatty acids.

Tricyclic boronic acid derivative, and preparation method therefor and application thereof

Disclosed are a tricyclic boronic acid derivative, and a preparation method therefor and an application thereof. Specifically, discloses are a compound represented by formula (I), an optical isomer and pharmaceutically acceptable salt thereof, and an application of the compound as a β-lactamase inhibitor and an application of a composition as an antibacterial drug.

Multi-factor authentication and access control in a vehicular environment

The systems and methods described herein can include a digital assistant application that receives sensor signals from sensors installed in a vehicle and determines an entry event into the vehicle. The digital assistant application can receive, responsive to the entry event into the vehicle, a plurality authentication input signals from a plurality of sensors associated with the vehicle. The digital assistant application can determine a plurality of authentication states based on the plurality of authentication input signals and a plurality of authentication credentials. The digital assistant application can identify an access permission level of a plurality of access permission levels based at least in part on the plurality of identifies authentication states. The digital assistant application can identify, responsive to the access permission level, a subset of a set of functionalities available via the vehicle, and provide vehicular access to the subset of functionalities.

Wig head mannequin

准直器车用于固定准直器的锁紧结构

本实用新型公开了准直器车用于固定准直器的锁紧结构，所述支撑板的上表面焊接有L形固定板，所述L形固定板的一侧焊接有固定块，所述固定块的内部开设有第二通孔，所述固定块的内部设置有销钉，所述销钉的一侧贯穿第二通孔的内部；推入时，将通过销帽将销钉拔出，通过惯性准直器上的定位板对止爪进行按压，止爪按压第一弹簧，到准直器确定的位置，第一弹簧的弹性势能使止爪复位，并将销钉插入第三通孔的内部，对准直器进行锁紧固定，推出时，将通过销帽将销钉拔出后向下按压止爪，然后推动准直器向外移动，第一弹簧的弹性势能使止爪复位，方便工作人员将准直器安装或拆卸在准直器车上，且便于单人操作该装置，省时省力，提高了使用效率。

A method for the site-specific enzymatic labelling of nucleic acids in vitro by incorporation of unnatural nucleotides

Provided herein are analogs of unnatural nucleotides bearing predominantly hydrophobic nucleobase analogs that form unnatural base pairs during DNA polymerase-mediated replication of DNA or RNA polymerase-mediated transcription of RNA. In this manner, the unnatural nucleobases can be introduced in a site-specific way into oligonucleotides (single or double stranded DNA or RNA), where they can provide for site-specific cleavage, or can provide a reactive linker than can undergo functionalization with a cargo-bearing reagent by means of reaction with a primary amino group or by means of click chemistry with an alkyne group of the unnatural nucleobase linker.

Reagents and methods for replication, transcription, and translation in semi-synthetic organisms

Disclosed herein are compositions, methods, cells, engineered microorganisms, and kits for increasing the production of proteins or polypeptides comprising one or more unnatural amino acids. Further provided are compositions, cells, engineered microorganisms, and kits for increasing the retention of unnatural nucleic acids encoding the unnatural amino acids in an engineered cell, or semi-synthetic organism.

Tricyclic boronic acid derivative, and preparation method therefor and application thereof

Disclosed are a tricyclic boronic acid derivative, and a preparation method therefor and an application thereof. Specifically, discloses are a compound represented by formula (I), an optical isomer and pharmaceutically acceptable salt thereof, and an application of the compound as a β-lactamase inhibitor and an application of a composition as an antibacterial drug.

Systems and methods for authenticating a user through an unobservable re-authentication system

Systems and methods for authenticating a user through an unobservable re-authentication process are disclosed.

Isobaric tandem mass tags for quantitative proteomics and peptidomics

Compositions and methods of tagging peptides and other molecules using novel isobaric tandem mass tagging reagents, including novel N,N-dimethylated amino acid 8-plex and 16-plex isobaric tandem mass tagging reagents. The tagging reagents comprise: a) a reporter group having at least one atom that is optionally isotopically labeled; b) a balancing group, also having at least one atom that is optionally isotopically labeled, and c) an amine reactive group. The tagging reagents disclosed herein serve as attractive alternatives for isobaric tag for relative and absolute quantitation (iTRAQ) and tandem mass tags (TMTs) due to their synthetic simplicity, labeling efficiency and improved fragmentation efficiency.