Riemerella anatipestifer detection kit and method based on loop-mediated isothermal amplification technology

The invention discloses riemerella anatipestifer detection kit and method based on a loop-mediated isothermal amplification technology. Based on six specific regions of a 16S rRNA conserved region of riemerella anatipestifer, two specific primers and two specific outer primers are designed in the kit and can specifically distinguish the riemerella anatipestifer and other serotype salmonellae so as to ensure the high specificity and the reliability of a detection result of loop-mediated isothermal amplification. The invention detects the riemerella anatipestifer on the basis of the loop-mediated isothermal amplification technology, can amplify a target sequence rapidly, efficiently and specifically under the isothermal condition, has simple and convenient operation and does not use expensive instruments and reagents; an amplification product can be developed directly by using a fluorescent dye and a result can be judged with naked eyes; the detection cost is low; and the invention is particularly ...

Production method of 5754 aluminum alloy blank using cast rolling machine

Traditional Chinese medicine feed additive for treating hypogonadism of male breeding stock and preparation method thereof

The invention provides a traditional Chinese medicine feed additive for treating hypogonadism of male breeding stock and a preparation method thereof. The feed additive is prepared from the following raw medicines: drying rehmannia root, herba cistanches, fructus cannabis, prepared rhizome of rehmannia, wolfberry fruit, actinolite, herba epimedii, schisandra chinensis, morindae radix, cuscuta and rhizoma curculiginis. The preparation method is characterized by mixing and then grinding the medicaments or grinding and then mixing the medicaments to prepare the powdered feed additive. The traditional Chinese medicine feed additive has obvious health care and treatment effects on oligospermia and asthenospermia of the male breeding stock and obviously improves the fertility of the breeding stock.

Propolis injection for preventing duck hemorrhagic oophoritis and preparation method thereof

The invention relates to the field of animal prevention medicine, particularly to a preparation method for an injection for preventing animal epidemic diseases from occurring, wherein the propolis injection for preventing duck hemorrhagic oophoritis is composed of a propolis aqueous solution, a duck embryo homogenated tissue inoculated with duck flavivirus-Tembusu virus and dead by infection, andan inactivation solution; the preparation method comprises the step of adding the inactivation solution in the mixed solution of the duck embryo homogenated tissue and propolis to obtain the propolisinjection for preventing duck hemorrhagic oophoritis; the propolis injection for preventing duck hemorrhagic oophoritis prepared by the preparation method of the invention has a remarkable effect to prevent duck hemorrhagic oophoritis (duck Tembusu virus disease); the protection ratio is increased to 90-100%, the protection period is prolonged to 6-12 months, the action time is shortened to 5 days, and ...

Casting rolling roller surface treatment method of aluminum casting rolling machine

The invention relates to a casting rolling roller surface treatment method of an aluminum casting rolling machine, which comprises the following steps: 1) cleaning the surface of a casting rolling roller by a solution prepared from methanol and nitric acid according to a volume ratio of 5 to 20/1, and forming an Fe2O3 ferric oxide covering layer with the uniform thickness between 0.05 and 0.1 mm on the surface of the roller; 2) using alcohol for dissolving colloidal graphite, then, adding water to prepare graphite lubrication liquid according to a volume ratio of colloidal graphite/ alcohol/water=1/0.5 to 2/20 to 60, and spraying the graphite lubrication liquid onto the surface of the casting rolling roller; and 3) heating circulated water by steam so that the water temperature can reach 30 to 70 DEG C, introducing the water into the inside of the casting rolling roller to cyclically heat the casting rolling roller, and then, drying the surface of the roller. The casting rolling roller ...

Traditional Chinese medicine composite for treating infectious bursal disease and preparation method thereof

The invention discloses a traditional Chinese medicine composite for treating infectious bursal disease and a preparation method thereof. The traditional Chinese medicine composition is prepared from glossy privet fruit, wolfberry fruit, dodder and astragalus root according to certain proportions, and has simple formula, low cost and stable quality. When used for treating the infectious bursal disease, the traditional Chinese medicine composite can effectively treat the infectious bursal disease, the effective rate is 91.7-94.2%, the death rate of infected chicken is reduced to 31.7-37.5%, and the traditional Chinese medicine composite not only has exact curative effect but also has no toxic and side effects and drug residues, improves the cultivation benefit and simultaneously avoids causing body harm to people who eat chicken. The traditional Chinese medicine composite is prepared by the steps of pulverizing the raw materials, leaching and concentrating the leaching solution, has stable ...

Duck enteritis virus detection kit and method based on loop-mediated isothermal amplification technology

The invention discloses duck enteritis virus detection kit and method based on a loop-mediated isothermal amplification technology. Based on six specific regions of a gene conserved region of a duck enteritis virus, two specific primers and two specific outer primers are designed in the kit so as to ensure the high specificity and the reliability of a detection result of loop-mediated isothermal amplification. The invention detects the duck enteritis virus on the basis of the loop-mediated isothermal amplification technology, can amplify a target sequence rapidly, efficiently and specificallyunder the isothermal condition, has simple and convenient operation, and does not use expensive instruments and reagents; an amplification product can be developed directly by using a fluorescent dyeand a result can be judged with naked eyes; the detection cost is low; and the invention is particularly suitable for small and medium size units and field tests.

4-carbostyril and 4H-1,4-benzothiazine derivatives and preparation method and application thereof

The invention relates to the field of medicinal chemistry, in particular to 4-carbostyril and 4H-1,4-benzothiazine derivatives, which are products obtained by structural modification of 3 position and 6 position of 4-carbostyril and parent nucleus. The invention also discloses a preparation method for the 4-carbostyril and 4H-1,4-benzothiazine derivatives, and application of the derivatives in sedation and hypnosis.

Propolis injection for preventing type I duck virus hepatitis and preparation method thereof

The invention relates to the field of animal prevention medicine, particularly to a preparation method for an injection for preventing animal epidemic diseases from occurring. The propolis injection for preventing type I duck virus hepatitis is composed of propolis aqueous solution, duck embryo homogenated tissue inoculated with duck hepatitis virus and dead owing to infection, and inactivation solution; the preparation method comprises the step of adding the inactivation solution in the mixed solution of the duck embryo homogenated tissue and propolis to obtain the propolis injection for preventing type I duck virus hepatitis; the propolis injection for preventing type I duck virus hepatitis prepared by the preparation method of the invention has remarkable effect to prevent duck virus hepatitis; the protection ratio is increased to 90-100%, the protection period is prolonged to 6-12 months, the action time is shortened to 5 days, and injecting once only can achieve the expected effect ...

Goose parvovirus detection kit and method based on loop-mediated isothermal amplification technology

The invention discloses goose parvovirus detection kit and method based on a loop-mediated isothermal amplification technology. Based on six specific regions of a VP3 gene conserved region of a goose parvovirus, two specific primers and two specific outer primers are designed in the kit so as to ensure the high specificity and the reliability of a detection result of loop-mediated isothermal amplification. The invention detects the goose parvovirus on the basis of the loop-mediated isothermal amplification technology, can amplify a target sequence rapidly, efficiently and specifically under the isothermal condition, has simple and convenient operation and does not use expensive instruments and reagents; an amplification product can be developed directly by using a fluorescent dye and a result can be judged with naked eyes; the detection cost is low; and the invention is particularly suitable for small and medium size units and field tests.

Salmonella enteritidis detection reagent kit and method based on loop-mediated isothermal amplification technology

The invention discloses salmonella enteritidis detection reagent kit and method based on a loop-mediated isothermal amplification technology, wherein two specific inner primers and two specific outer primers are designed by the reagent kit according to the six specific regions of a salmonella enteritidis Sdf I gene conserved region, thereby ensuring the specificity of loop-mediated isothermal amplification and the reliability of a detecting result; in the invention, salmonella enteritidis is detected on the basis of the loop-mediated isothermal amplification technology, a target sequence can be rapidly, efficiently and specifically amplified under an isothermal condition, the operation is simple and convenient, expensive instruments and reagents are not needed, an amplified product is directly developed through fluorescent dye, a result can be judged by naked eyes, and the detection cost is low; and the reagent kit and the method can specifically distinguish serum specific type salmonella ...

Riemerella anatipestifer detection kit and method based on loop-mediated isothermal amplification technology

The invention discloses riemerella anatipestifer detection kit and method based on a loop-mediated isothermal amplification technology. Based on six specific regions of a 16S rRNA conserved region of riemerella anatipestifer, two specific primers and two specific outer primers are designed in the kit and can specifically distinguish the riemerella anatipestifer and other serotype salmonellae so as to ensure the high specificity and the reliability of a detection result of loop-mediated isothermal amplification. The invention detects the riemerella anatipestifer on the basis of the loop-mediated isothermal amplification technology, can amplify a target sequence rapidly, efficiently and specifically under the isothermal condition, has simple and convenient operation and does not use expensive instruments and reagents; an amplification product can be developed directly by using a fluorescent dye and a result can be judged with naked eyes; the detection cost is low; and the invention is particularly ...

Traditional Chinese medicine composite for treating infectious bursal disease and preparation method thereof

The invention discloses a traditional Chinese medicine composite for treating infectious bursal disease and a preparation method thereof. The traditional Chinese medicine composition is prepared from glossy privet fruit, wolfberry fruit, dodder and astragalus root according to certain proportions, and has simple formula, low cost and stable quality. When used for treating the infectious bursal disease, the traditional Chinese medicine composite can effectively treat the infectious bursal disease, the effective rate is 91.7-94.2%, the death rate of infected chicken is reduced to 31.7-37.5%, and the traditional Chinese medicine composite not only has exact curative effect but also has no toxic and side effects and drug residues, improves the cultivation benefit and simultaneously avoids causing body harm to people who eat chicken. The traditional Chinese medicine composite is prepared by the steps of pulverizing the raw materials, leaching and concentrating the leaching solution, has stable ...

Casting rolling roller surface treatment method of aluminum casting rolling machine

The invention relates to a casting rolling roller surface treatment method of an aluminum casting rolling machine, which comprises the following steps: 1) cleaning the surface of a casting rolling roller by a solution prepared from methanol and nitric acid according to a volume ratio of 5 to 20/1, and forming an Fe2O3 ferric oxide covering layer with the uniform thickness between 0.05 and 0.1 mm on the surface of the roller; 2) using alcohol for dissolving colloidal graphite, then, adding water to prepare graphite lubrication liquid according to a volume ratio of colloidal graphite/ alcohol/water=1/0.5 to 2/20 to 60, and spraying the graphite lubrication liquid onto the surface of the casting rolling roller; and 3) heating circulated water by steam so that the water temperature can reach 30 to 70 DEG C, introducing the water into the inside of the casting rolling roller to cyclically heat the casting rolling roller, and then, drying the surface of the roller. The casting rolling roller ...