09-03-2017 дата публикации
Номер: US20170067011A1
A method for artificial cultivation of fruiting bodies. The method comprises: inoculating into a sterile rice medium, cultivating at 9-13° C. for 40-60 days, after the medium is covered with mycelia, performing low-temperature induction at 1-8° C. for 60-80 days to develop a fruiting body primordium, and transferring the cultivation to 11-16° C. till harvest of the fruiting bodies. The method provided by the present invention requires no low-oxygen environment, which can reduce cultivation cost; it only needs 3-4 months from induction to harvest of fruiting bodies; the rice medium for use has a low cost, which is suitable for commercial cultivation of fruiting bodies. 1Ophiocordyceps sinensis. A method for artificial cultivation of fruiting bodies , comprising the following steps:{'i': 'Ophiocordyceps sinensis', 'inoculating into a sterile cultivation medium, cultivating at 9-13° C. for 40-60 days, covering the sterile cultivation medium with mycelia and after the sterile cultivation medium is covered with mycelia, performing low-temperature induction at 1-8° C. for 60-80 days to develop a fruiting-body primordium, forming a cultivation and transferring the cultivation to 11-16° C. to harvest fruiting bodies;'}wherein the sterile cultivation medium is obtained by mixing rice with a nutrient solution by weight ratio of 1:1-1.5, and the nutrient solution, by a total mass fraction of 100%, comprises glucose 2%, KH2PO4 0.2%, MgSO4 0.1%, ammonium citrate 0,1%, peptone 0.5%, silkworm pupae powder 0.2%, and the a balance of water, with the a pH of 6.0-6.5.2Ophiocordyceps sinensisOphiocordyceps sinensis. The method for artificial cultivation of fruiting bodies according to claim 1 , wherein the is prepared by the following method:{'i': Ophiocordyceps sinensis', 'Ophiocordyceps sinensis', 'Ophiocordyceps sinensis, '(1) preparing a parent species inoculating into a solid PPDA medium, performing dark cultivation at 9-16° C. for 45-60 days, and then selecting typical colonies ...
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