Chicken pdx1 polyclonal antibody and application

The invention aims at providing a chicken pdx1 polyclonal antibody and application; the chicken pdx1 polyclonal antibody is caused by an immunogen sequence and can be specifically combined with surface molecules of stem cells of chicken pancrease; and the polyclonal antibody is used for detecting extracted protein of the stem cells of the chicken pancrease by immunoblotting so as to obtain a single specificity positive band. By immunohistochemical detection, the stem cells of chicken pancrease display specificity positive fluorescence.

SELF-PRESSURIZED RESILIENT GASKET

The present invention relates to the technical field of electric power system fasteners. A self-pressurized resilient gasket, comprising an annular gasket body (1); the gasket body (1) is symmetrically folded into a V-shape by utilizing the central axis as an axis of symmetry; the folded edges are the pressurization position (3); and the open edges are the supporting position (2); the V-shaped circular arc angle a of the gasket body (1) is 140°-160°, and the radius R of the circular arc is 10-30mm. The gasket body (1) is made of a memory alloy.

Circumcision apparatus and application method thereof

The invention relates to the technical field of medical instruments and discloses a circumcision apparatus and an application method thereof. The circumcision apparatus comprises a cylindrical inner ring made of polyethylene material and a clamp made of nickel-titanium memory alloy material; the outer wall of the cylindrical inner wall is covered with a silicone back-up ring; the clamp comprises a clamp body; the clamp body is in a rectangular elongated shape and in a ring shape with two ends fastened through a fastener and a fastening opening, or the clamp body is a ring having extension arcs radially projecting out. The application method of the circumcision apparatus includes the steps such as setting the clamp, clamping the foreskin, cutting off the redundant part of the foreskin, and performing binding. The clamp can auto-pressure and restore at 25 DEG C to 35 DEG C so as to fasten the foreskin to the inner ring, blood supply is effectively blocked, and dropping of the clamp is prevented ...

Multi-specification and multipurpose organization example embedding machine

This invention is frozen tissue sample section embed apparatus used for life science experiment and medical pathological tissue examination. A multi-gauge multi-use tissue sample embed apparatus is comprised of a multi-gauge embedding frame, an embedding substrate, a spherical connector. By designing tissue sample embedding apparatus with different size and gauge, problems with existing technology of difficulty on embedding tissue and embryo in different shape and size can be conveniently solved via using single case, which effectively avoid confusion among different tissue and samples. The apparatus according to this invention can conveniently and rapidly carry out high quality freezing process can be carried out on embedded tissue and embryo as a special tissue embed-freeze apparatus, especially suitable for life science, pathology and anatomy, etc., which simultaneously bring new improvement for existing tissue embedding module.

Manufacture method of antibacterial ultraviolet resisting electromagnetic shielding fabric

The invention relates to a manufacturing method of an antibacterial ultraviolet resisting electromagnetic shielding fabric, which solves the technical problem that the fabric has both electromagnetic shielding function and excellent antibacterial and ultraviolet resistance. The technical scheme solving the technical problem is achieved as follows: after an activated fabric is plated with a metal layer, a TiO2-PU coating is coated outside the metal layer. The invention can be used for the filed of the preparation the electromagnetic shielding materials.

Ear margin tissue fibroblast line of Minxian county black fur sheep and its culture process

Circumcision device

The utility model relates to the technical field of medical equipment, a circumcision device and application method is disclosed, circumcision device includes the cylindric inner ring that polyethylene material made, the binding clasp that titanium nickel memory alloy material was made, endocyclic outer wall parcel has the silicone gasket. The binding clasp is connected through dop, the bayonet socket lock of looks adaptation for the rectangular form of rectangle binding clasp body both ends and is the ring form, perhaps the ring of binding clasp body for having radial outside bellied expansion circular arc. Circumcision device's application method includes holder design, centre gripping foreskin, cut unnecessary foreskin, the step such as wrap. The binding clasp can the self -pressurization reconversion in the time of 25 DEG C - 35 DEG C, with the foreskin fastening on the inner ring, effectively block blood and supply, come off in preventing the activity, use the utility model discloses ...

Memory alloy surrounds formula thighbone coaptation ware and takes out instrument

The utility model discloses a memory alloy surrounds the instrument that takes out of formula thighbone coaptation ware, which comprises a bracket, the support lower extreme is installed at least two pairs and is taken out the hook, two of every centering takes out the hook and corresponds the setting each other, the both ends at support top are connected with the handle, the middle part of support is equipped with the driving gear, the driving gear passes through the drive arrangement drive and rotates, the driving gear both sides are equipped with the driven gear rather than the meshing on the support, a screw rod has been connect among each driven gear soon, two screw rod lower extremes are connected with a clamp plate, the clamp plate is located two of all mutual correspondences and takes out the hook that takes out that is used for strutting mutual correspondence between the hook. The utility model discloses can the problem that the coaptation ware takes place to be difficult for taking ...

Myocardial specificity homeobox part polypeptide and application thereof

The invention discloses a myocardial specificity homeobox part polypeptide and application thereof. The amino acid sequence of the myocardial specificity homeobox part polypeptide is a sequence I in a sequence table. The polypeptide and carrier protein are coupled to form composite protein. The carrier protein can be keyhole limpet hemocyanin or bovine serum albumin. A mixture of the composite protein and an immunologic adjuvant can be used as immunogen. Immune serum with higher valence is obtained by immunizing an animal with the immunogen, the valence is higher than 1: 62500 through ELISA (Enzyme-Linked Immuno Sorbent Assay) detection and the immune serum can detect the expression of an avian myocardial specificity homeobox on the cell level.

Method for detecting goat fertility

The invention discloses a method for detecting the productive capacity of a goat. The method is to detect the 757 bit nucleic acid of a GnRHR gene in the gene group of the detected goat to be G or be mutated into A and confirms the genotype of the goat, then confirms the productive capacity of the goat by the genotype; if the 757 bit nucleic acid of the GnRHR gene in the gene group of the detected goat is G, the genotype of the homozygote thereof is AA; when the 757 bit nucleic acid of the GnRHR gene in the gene group of the detected goat is A, the genotype of the homozygote thereof is BB; the genotype of the heterozygous thereof is AB; the productive capacity of the goat of BB genotype is better than that of the goats with AB genotype and AA genotype. The method of the invention utilizesinheritance to mark the polymorphism for confirming the productive capacity of the goat, can fast and conveniently confirm the productive capacity of the goat of Jining and provides an accurate and simple ...

Memory alloy alarm device in electromagnetism sensing cabinet

The utility model discloses a memory alloy alarm device in electromagnetism sensing cabinet, including the electric appliance cabinet box, set up intelligent signal device in the electric appliance cabinet box, intelligence signal device connects the sensor, the sensor is connected with the alarm, the alarm shows the output of overheated alarm signal and the signal output that normally works. Alarm device has that processing technology is simple, sensitivity is high in this electromagnetism sensing cabinet, corrosion -resistant, nonmagnetic, longe -lived, advantages such as the temperature sensing linearity is good, the security is high, the stable performance is reliable, longe -lived, energy saving, environmental protection, simple installation, its performance and manufacturability all are superior to the alarm device who uses other temperature sensing materials, therefore can keep the safety in production for the realization and provide effectual assurance.

Allotype enclasp type bone knitting plate with two or three girders

The double or three beam irregular clasping fracture settling board is one kind of medical equipment. The fracture settling board has several fixing parts with side teeth, two or three connecting beams between fixing parts and one stabilizing hooked nail connected via a rod to one of the fixing parts. The fracture settling board is one annular open-mouthed structure. The present invention features that the fracture settling board is made of shape memory alloy to facilitate bone settling operation, is suitable for physiological characteristic of bones, and has one stabilizing hooked nail and bone end pressurizing hooked claw for reliable fixation.

'Jingning' chicken embryo fibroblast series and its culture method

Automatic pressing dynamic hip riveting device

The invention discloses an automatic pressing dynamic hip riveting device, which comprises a rivet, a sleeve, a tubular memory alloy spring and a nut, wherein the tail end of the rivet is provided with external screw threads, a hollowed hole is formed at the lower end of the sleeve; the tubular memory alloy spring is sleeved in the sleeve; an inner hexagonal bore is formed in the upper end of the rivet, while screw threads are formed outside the upper end of the rivet; the upper part of the rivet is arranged in the sleeve, the memory alloy spring and the hollowed hole; and the nut is arranged outside the screw threads on the upper end of the rivet and positioned in the sleeve. The automatic pressing dynamic hip riveting device automatically presses the broken end of a bone to promote bone growth and accelerate coalescence, and thus, the nonunion phenomenon is effectively avoided.

Graphite frame bearing hooks

The utility model relates to a solar cell prepares technical field, especially relates to a graphite frame bearing hooks, including crossbeam, side arm and bracing piece, the side arm is two, locatesthe crossbeam both ends respectively perpendicularly, the bracing piece is two, locates the bottom of side arm respectively perpendicularly, side arm and the inboard arch that is used for the card tokeep off graphite frame that is equipped with of bracing piece handing -over department, the utility model provides a bearing hooks change inconvenient problem, improved work efficiency.

Tianzhushan white yak ear-edge tissue fiber cell system and constituting method

The invention relates to a method for establishing and culturing Tianzhu Yak'ear marginal fibroblast cell line, utilizing the fast tissue wall pasting method to improve culture medium, and finally obtaining high-yield and high-purity Tianzhu Yak'ear marginal fibroblast cell line whose conservation number is CGMCC No.1881, belonging to the field of cell biology. And the frozen cells have stable quality, suitable to large scale culture. And the Tianzhu Yak'ear marginal fibroblast cell line can provide large numbers of high quality materials for research on science of living systems in gene engineering, cell engineering, immunity and molecular biology; can act as donor cells for animal's somatic cell cloning and breeding; and can enrich local variety improvement and storage of local excellent variety in agriculture.

A spanner for proofreading and correct tubular PECVD graphite boat rhombus stuck point

The utility model discloses a spanner for proofreading and correct tubular PECVD graphite boat rhombus stuck point relates to solar cell preparation technical field, mainly includes the base plate, the base plate is opened near the lower extreme position has a plurality of rhombus bayonet socket, the angle inclination of a plurality of bayonet sockets is all inequality, the base plate is close tothe upper end position and still is provided with a plurality of sign, a plurality of signs and a plurality of rhombus bayonet socket one -to -one set up a plurality of rhombus bayonet sockets on samespanner, can be directed against the rhombus stuck point of a plurality of positions, convenient and practical, and the spanner is made by panel, can make things convenient for it to stretch into inthe graphite boat gap, still be provided with the sharp sign corresponding with the rhombus bayonet socket, the convenience is fixed a position the rhombus stuck point.

Activation method before flexible backing material chemical plating based on numerator self-assembly technique

The invention provides a molecular self-assembly technique based method for pre-activating a flexible base material before chemical plating, comprising the following steps of: 1. placing a crosslinking agent and chitosan or derivative thereof into a weak acidic aqueous solution, with electromagnetical stirring and keeping the solution stand to obtain a chitosan finishing liquid; 2. impregnation method: impregnating a fabric in the chitosan finishing liquid with electromagnetical stirring; cleaning the fabric with deionized water and drying the fabric; a padding method: impregnating the fabric in the chitosan finishing liquid, and carrying out impregnating and padding twice with a padder as well as pre-drying and baking to the fabric; 3. placing the fabric in an activation solution with palladium chlonde; route 1: activating, cleaning with the deionized water and drying; route 2: stirring electromagnetically or keeping stand, and baking. The method has the advantages of improving friction ...

Method for preparing carbon black through wet granulation

The invention discloses a method for preparing carbon black through wet granulation. The method comprises the steps of splitting decomposition of raw oil, separation of carbon black smoke, granulation and drying, wherein tail gas generated in the splitting decomposition of the raw oil is combusted in a tail gas furnace to provide a heat source for the drying process; the temperature in the drying process is controlled according to the amount of the tail gas supplied to the tail gas combustion furnace of a drier; and the amount of the tail gas supplied to the tail gas combustion furnace of the drier is controlled through a tail gas control program. By adopting the method, product combustion caused by a little over-high temperature during the drying process of the carbon black or package burning caused during a packaging process can be avoided, and disqualification of the moisture in the carbon black caused by over-low temperature can be avoided, so that stable production of qualified granular ...

Flexible fracture setting claw

The flexible fracture setting claw belongs to medical internally fixture. Steel wire is formed to circular part and fixing claw, which form the fracture setting claw with curvature similar to that ofbone to be set, and the fixing claws are formed in the outuper part between two rings. The present invention is flexible and adaptive to complicated bone shape and can balance the tensions of the claws.

Pair of fever abatement massage gloves for children

The utility model relates to a pair of fever-reducing massage gloves for children, which comprises a pair of glove bodies correspondingly matched with two hands of the children, lining sleeves are sewed on the inner walls of the glove bodies, a plurality of massage clamping pieces are sewed and fixed in interlayers between the lining sleeves and the glove bodies, each massage clamping piece comprises a circular bottom piece, and the outer surface of each circular bottom piece is a plane. Massage protrusions with the high center and the low periphery are arranged on the inner surface of the round bottom piece, the top points of the massage protrusions are in one-to-one correspondence with an inner laogong acupoint, an outer laogong acupoint, a total tendon acupoint, a lung gold acupoint, a kidney water acupoint, a kidney line acupoint, a plank acupoint, two door acupoints and two upper horse acupoints, and point marks are arranged on the outer surface of the glove body. The massage protrusions ...

Method for excavating sheep skeletal muscle development related genes based on MeRIP-Seq technology and application

The invention provides a method for excavating sheep skeletal muscle development related genes by a MeRIP-Seq technology and application of the method. Firstly, RNA specific m6A antibody immunoprecipitation, library construction and sequencing are sequentially carried out on extracted sheep longissimus dorsi tissue RNA, and obtained original data are subjected to quality control filtration; the method comprises the following steps of: filtering data of sheep, comparing the filtered data with reference genome data of the sheep, performing m6A peak identification and annotation on genome comparison position data to obtain differential m6A peak related genes, and identifying the differential m6A peak related genes and performing GO and KEGG function enrichment analysis on the differential m6A peak related genes. From the perspective of epigenetics, the invention provides a scientific basis for exploring the growth and development regulation mechanism of the sheep skeletal muscle, and has important ...

Manufacture method of antibacterial ultraviolet resisting electromagnetic shielding fabric

The invention relates to a manufacturing method of an antibacterial ultraviolet resisting electromagnetic shielding fabric, which solves the technical problem that the fabric has both electromagnetic shielding function and excellent antibacterial and ultraviolet resistance. The technical scheme solving the technical problem is achieved as follows: after an activated fabric is plated with a metal layer, a TiO2-PU coating is coated outside the metal layer. The invention can be used for the filed of the preparation the electromagnetic shielding materials.

Electric power system high -low pressure position automatic indicator that calorifys

The utility model provides an electric power system high -low pressure position automatic indicator that calorifys simple structure, it is small, just with transportation and installation. With tiNi two -way shape memory alloy intelligence spring temperature -sensing element, make tiNi two -way shape memory alloy intelligence spring take place mechanical motion by the temperature on the easy exothermic contact of tiNi two -way shape memory alloy intelligence spring perception and come that control is red indicates on popping out of flag show easy exothermic contact. Electric power system high -low pressure position automatic indicator that calorifys does not need the energy, and the restriction that does not receive ambient temperature, humidity, wind speed and shelter from ambient condition such as thing can be for realizing that energy saving and control contact generate heat the drawback and provide the efficient and guarantee, the electric power system junction of being applied to that ...

Electro-adsorber, device and extraction method for in-situ extraction of salt lake cesium ions

The invention discloses an electro-adsorber for in-situ extraction of salt lake cesium ions. The electro-adsorber comprises a box body, an electro-adsorption unit, a water inlet and a water outlet, the electro-adsorption unit comprises an adsorption electrode, a metal electrode and an adsorption space formed between the adsorption electrode and the metal electrode, the adsorption electrode and the metal electrode are of a plate-shaped structure arranged in parallel, and the adsorption electrode and the metal electrode are insulated; the material of the adsorption electrode is Na2Ti3O7; the metal electrode is an inert electrode. The invention further discloses a device for extracting the cesium ions in the salt lake in situ. The device comprises an electric adsorber, a raw water tank, a regenerated water tank, a desorption water tank and a cesium-rich water tank, the invention also discloses a method for extracting cesium ions by using the device. The method has the advantages that in-situ ...

Grouting pump flow and flow proportion control method

Disclosed is a grouting pump flow and flow proportion control method. The method includes that an electromotor in a hydraulic pressure station is controlled through an inverter and drives a hydraulic pump of the hydraulic pressure station, the hydraulic pump supplies oil to a first three-position four-way electromagnetic directional valve and a second three-position four-way electromagnetic directional valve to control motion of a first hydraulic cylinder and a second hydraulic cylinder, feeding, discharging and stopping of a first grouting pump body and a second grouting pump body are realized, and when the first grouting pump body and the second grouting pump body are fed, feeding is performed through liquid inlets of the first grouting pump body and the second grouting pump body. The grouting pump flow and flow proportion control method is characterized in that when the first grouting pump body and the second grouting pump body are discharged, in other words, double liquid are mixed ...

Method for preparing finishing agent of modified nano titanium dioxide coating layer of electromagnetic shielding fabric

The invention relates to a method for preparing a finishing agent of a modified nano titanium dioxide coating layer of an electromagnetic shielding fabric. The technical problem of the invention to be solved is that the method for preparing the finishing agent of the modified nano titanium dioxide coating layer of the electromagnetic shielding fabric is provided, so that the electromagnetic shielding fabric has excellent performance of ultraviolet ray resistance, antibacterium, self-cleaning and the like, and the original characteristic of low surface resistance can be maintained at the same time. The technical scheme for solving the problem is that nano TiO2 is modified by a special process, and then the modified nano TiO2 is mixed uniformly with resin and a cross-linking agent according to the proportion, so that the finishing agent of the modified nano titanium dioxide coating layer of the electromagnetic shielding fabric is obtained. The invention can be used for the textile industry ...

Method for identifying Tan sheep based on genotyping

The invention discloses a method for identifying Tan sheep based on genotyping. The method comprises the following steps: detecting nucleotide at each SNP site in a specific SNP site combination in a genome of a to-be-detected sheep, and determining a genotype of the to-be-detected sheep corresponding to the specific SNP site combination; determining whether the to-be-detected sheep is the Tan sheep according to the following steps: if the genotype of the to-be-detected sheep corresponding to the specific SNP site combination conforms to the standard genotype of the Tan sheep, determining that the to-be-detected sheep is the Tan sheep or a candidate Tan sheep; if the genotype, corresponding to the specific SNP site combination, of the to-be-detected sheep conforms to the standard genotype of the non-Tan sheep, the to-be-detected sheep is the non-Tan sheep or the candidate to be the non-Tan sheep. The SNP locus combination for identifying the Tan sheep and the non-Tan sheep is successfully ...

Polypeptide in cluster-of-differentiation part on surface of vascular endothelial cell and use thereof

The invention discloses a polypeptide in cluster-of-differentiation (CD) 34 part of a chicken, wherein the amino acid sequence of the polypeptide is Cys Gln Ser Ser Ala HisPro Ser Thr His Pro Ser Val His Pro Ser Thr His. The polypeptide in cluster-of-differentiation part on the surface of a vascular endothelial cell, which is provided by the invention, has high specificity and high sensitivity.

Liancheng white duck embryo fibroblast and culture method thereof

Memory alloy artificial cervical intervertebral disc

A memory alloy artificial cervical intervertebral disc comprises an artificial intervertebral disc body and a support plate sleeved with the artificial intervertebral disc body, wherein the artificial intervertebral disc body has conicity; drop preventing reverse stings are distributed on the upper and lower surfaces of the artificial intervertebral disc body; fixed branches are arranged at the two sides of the upper and lower surfaces; and drop preventing pawls are respectively arranged at the two ends at the inner side of each fixed branch. The intervertebral disc has simple process and shape, is easy to deform and insert, is stable in installation after complex, is convenient to use in operation, is secure and reliable in fixation, maintains the cervical vertebra motion range, reduces adjacent vertebral body complications, not only shortens the operation time and simplifies the operation, but also dispenses with bone removal and grafting, reduces more pains of the patient, maintains ...

Portable rechargeable lithium -ion battery rail bonding drill

The utility model provides a portable rechargeable lithium -ion battery rail bonding drill, includes: the support is provided with the connecting plate on, the connecting plate is provided with calliper, is provided with the connecting axle on the support, drill bit, drill bit are arranged in on the connecting plate, ejector pin, ejector pin rotate with the support to be connected, and the ejector pin is provided with adjustment screw and rand, and the rand sets up with calliper relatively, bracket component, bracket component include upper bracket and lower carriage, and the upper bracket is connected with the support, are provided with feeding device on the upper bracket, and the lower carriage passes through feeding device and upper bracket sliding connection, drive arrangement, drive arrangement is 48v, operating power for 1000W's direct current motor for power voltage, can the source module be 48v's lithium phosphate battery for power supply voltage. The utility model discloses simple ...

Circular RNA (Ribonucleic Acid) related to curving of sheep hair and application of circular RNA

The invention relates to the technical field of biology, and particularly provides circular RNA (Ribonucleic Acid) related to curving of sheep hair and application of the circular RNA. According to the invention, circRNA of skin tissues with different hair bending phenotypes at different development stages of the Chinese and defense goats is identified and analyzed by using transcriptome sequencing, the function of the circRNA is researched at the cellular level, and circRNA which obviously affects hair bending and hair follicle development is screened. The application of the non-coding circular RNA circRNA8782 in inhibition of proliferation of sheep skin fibroblasts is found for the first time, and a molecular basis is provided for circRNA to participate in regulation and control of growth and development of hair follicles, hair bending and the like.

Vascular endothelial growth factor receptor partial polypeptide and application thereof

The invention discloses a vascular endothelial growth factor receptor partial polypeptide and application thereof. The amino acid sequence of the vascular endothelial growth factor receptor partial polypeptide is Sequence 1 in the sequence table. The polypeptide can be coupled with a carrier protein to form a compound protein. The carrier protein is a keyhole limpet hemocyanin or bovine serum albumin. The mixture of the compound protein and an immunological adjuvant can be used as an immunogen. The immunogen immunized animal acquires high-titer immune serum. The ELISA inspection indicates that the titer is higher than 1:60000; and the immunobiotting test proves that the immune serum has strong specificity when being combined with a chicken vascular endothelial growth factor receptor, and can be used for detecting the expression of the chicken vascular endothelial growth factor receptor on the cellular level.

Antibiotic finishing process on conductive fabric by adopting sol-gel method

The invention relates to an antibiotic finishing process on conductive fabric by adopting a sol-gel method, aiming at solving the technical problem for providing an antibiotic finishing process which enables the conductive fabric to maintain an original electromagnetic shielding performance while having an excellent antibiotic function. The technical scheme for solving the technical problem comprises the steps of: firstly, activating fabric, placing the activated fabric into a chemical plating solution, stirring under an electromagnetic condition at 60 DEG C for reacting for 30min, water washing and baking to prepare the conductive fabric; then preparing zinc oxide hydrosol; finally, after water washing the conductive fabric, immersing the conductive fabric into the zinc oxide hydrosol twice, wherein liquor detention is 80 percent; and baking the conductive fabric at 80-100 DEG C, and then baking for 10-30min at 110-160 DEG C to prepare the antibiotic finished conductive fabric. The invention ...

Method for identifying Tan sheep and non-Tan sheep based on genotyping

The invention discloses a method for identifying Tan sheep and non-Tan sheep based on genotyping. The method comprises the following steps: detecting nucleotide at each SNP site in a specific SNP site combination in a genome of a to-be-detected sheep, and determining a genotype of the to-be-detected sheep corresponding to the specific SNP site combination; determining whether the to-be-detected sheep is the Tan sheep according to the following steps: if the genotype of the to-be-detected sheep corresponding to the specific SNP site combination conforms to the standard genotype of the Tan sheep, determining that the to-be-detected sheep is the Tan sheep or a candidate Tan sheep; if the genotype, corresponding to the specific SNP site combination, of the to-be-detected sheep conforms to the standard genotype of the non-Tan sheep, the to-be-detected sheep is the non-Tan sheep or the candidate to be the non-Tan sheep. The SNP locus combination for identifying the Tan sheep and the non-Tan sheep ...

Method for identifying Tan sheep and non-Tan sheep based on genotyping

The invention discloses a method for identifying Tan sheep and non-Tan sheep based on genotyping. The method comprises the following steps: detecting nucleotide at each SNP site in a specific SNP site combination in a genome of a to-be-detected sheep, and determining a genotype of the to-be-detected sheep corresponding to the specific SNP site combination; determining whether the to-be-detected sheep is the Tan sheep according to the following steps: if the genotype of the to-be-detected sheep corresponding to the specific SNP site combination conforms to the standard genotype of the Tan sheep, determining that the to-be-detected sheep is the Tan sheep or a candidate Tan sheep; if the genotype, corresponding to the specific SNP site combination, of the to-be-detected sheep conforms to the standard genotype of the non-Tan sheep, the to-be-detected sheep is the non-Tan sheep or the candidate to be the non-Tan sheep. The SNP locus combination for identifying the Tan sheep and the non-Tan sheep ...

Nestin portion polypeptide and application thereof

The invention discloses a nestin portion polypeptide and applications thereof. The nestin portion polypeptide of the invention has an amino acid sequence of sequence 1 in the sequence table. The coupling of the polypeptide with carrier protein can form composite protein. The carrier protein can be keyhole limpet hemocyanin or bovine serum albumin. A mixture mixed by the composite protein and an immune adjuvant can be used as an immunogen. Animals immunized by the immunogen of the invention have immune serum with relatively high titer which is determined to be higher than 1:62500 by ELISA assay, and the immune serum can detect the expression of nestin of avian origin at cell level.

Separation and culture method of epidermal stem cells of Beijing ducks

The invention provides a separation and culture method of epidermal stem cells of Beijing ducks. The separation and culture method comprises the following steps of: preparation of materials, washing, digesting, primary culture and subculture. The epidermal stem cells of the Beijing ducks prepared by the method can provide seed cells for life science researches in tissue engineering, gene engineering, cell engineering, immunology, molecular biology and the like, can enrich and improve local varieties in agriculture and open up a novel way in the aspect of protection of the genetic diversity oflivestock and poultry, and can be taken as main raw materials for producing vaccines.

Mount Wuzhi Pig ear rim tissue fibroblast series and culturing method thereof

The invention discloses a high-living rate and high-purity mature fiber cell system of Mongolian horse (CGMCC No.1879) in the cellar biological domain, which is characterized by the following: culturing high-purity phoroblast without epithelial cell; maintaining the living rate of frozen cell between 93.2% and 98.7%; fitting for large scale of culturing.

TDI (toluene diisocynate) production wastewater treatment system and process

The invention belongs to the technical field of wastewater treatment, and particularly relates to a TDI production wastewater treatment system and a TDI production wastewater treatment process, the TDI production wastewater treatment system comprises a homogenizing tank 1, a low-temperature catalytic oxidation unit, a homogenizing tank 2, an anaerobic tank, an A/O-MBBR biological reaction tank, an A/O biological reaction tank, a sedimentation tank and a monitoring water tank which are sequentially arranged along the wastewater treatment direction. According to the method, nitrobenzene and aniline compounds in TDI production wastewater are efficiently degraded, the biotoxicity of the wastewater is reduced, the biodegradability of the wastewater is improved, meanwhile, the concentration of COD in the wastewater is reduced, secondary pollution such as iron mud is not generated in the using process, effluent reaches the discharge standard, and pollution is avoided. The invention also provides ...

SELF-PRESSURIZED RESILIENT GASKET

The present invention relates to a self-pressurized resilient gasket, which belongs to the field of electric power system fasteners, and comprises an annular gasket body. The gasket body is symmetrically folded into a V-shape by utilizing the central axis as an axis of symmetry, the folded edges are the pressurization position and the open edges are the supporting position. The V-shaped circular arc angle α of the gasket body is 140°-160°, and the radius R of the circular arc is 10 mm-30 mm. The self-pressurized resilient gasket is made of a memory alloy, and utilizes the characteristics of the memory alloy—memory performance and superelasticity characteristic to effectively solve the problems that common 65 Mn and 60 Si2MnA spring gaskets may lose elasticity in case of being under a higher temperature frequently, thus losing the effect of preventing a fastened piece from being loosened, so that the fastened piece is loosened and contact resistance is increased due to poor contact, thus causing heating more easily, which burns the fastener and the fastened piece, and thereby causing faulted power failure. The self-pressurized resilient gasket saves a number of manpower and material resources required by regularly replacing all the fasteners, and has very good economic and social benefits.

Multifunction organization embedding and refrigerating plant

This invention is a tissue embed-freeze apparatus for frozen tissue section used for life science experiment and medical pathological tissue examination, comprised of a multifunctional liquid nitrogen storage thermostatic bottle and an adjustable suspending water tank. The multifunctional liquid nitrogen storage thermostatic bottle is internally designed with a conical baffle for the adjustable suspending water tank to enter, used for freezing the embedded tissue and reducing volatilization of liquid nitrogen therein. The adjustable suspending water tank is disposed with two freely operable pull rods on two sides thereof, in order to adjust immerging depth and position of the water tank into the liquid nitrogen in the thermostatic bottle within 0 to 30cm according to actual need. With utilization, this invention can rapidly and conveniently carry out high quality freezing process can be carried out on embedded tissue and embryo as a special tissue embed-freeze apparatus, especially suitable ...

Over-break and under-break control method for mechanical construction of large-section tunnel in complex geology

The invention belongs to the technical field of tunnel construction, and particularly relates to a complex geology large-section tunnel mechanical construction over-break and under-break control method which comprises the steps that S1, a positioning device is erected in a tunnel, the height of an arch frame is adjusted, and the axis of the arch frame and the axis of the tunnel are kept coincident; s2, laser is emitted to the tunnel face through a plurality of laser emitters at the same time, a plurality of laser projection points are formed at the edge position of the tunnel face, and the positions of edge blast holes are marked; s3, a plurality of edge blast holes are drilled in the tunnel face, then other middle blast holes are drilled, and then explosives are arranged in all the blast holes and detonated; according to the control method, the convenience of laser positioning is utilized, the positioning work of the edge blast hole is simplified, the shape of the arch frame is matched ...

Complementary deoxyribonucleic acid (cDNA) library of ear marginal tissues of small tailed han sheep and construction method thereof

The invention relates to a complementary deoxyribonucleic acid (cDNA) library of ear marginal tissues of small tailed han sheep and a construction method thereof. The ear marginal tissues of the small tailed han sheep are taken as materials, and the high-quality cDNA library of the ear marginal tissues of the small tailed han sheep, which has high titer and recombination frequency and a wide insert fragment range, is prepared by total ribonucleic acid (RNA) extraction, double-chain cDNA synthesis, proteinase K digestion, Sfi 1 enzyme cutting and vector connection, and lambda phage packaging.

Mongolian horse ear rim tissue fibroblast series and culturing method thereof

The invention discloses a high-living rate and high-purity mature fiber cell system of Mongolian horse (CGMCC No.1876) in the cellar biological domain, which is characterized by the following: culturing high-purity phoroblast without epithelial cell; maintaining the living rate of frozen cell between 93.2% and 98.7%; fitting for large scale of culturing.

Method for preparing anode catalyst of direct methanol fuel cell

The invention relates to a method for preparing an anode catalyst of a direct methanol fuel cell. The method comprises the following steps of: dispersing and dissolving carbon black, SnCl4.5H2O and SbCl3 in absolute ethanol; filtering, washing, drying, grinding and calcining to obtain an antimony tin oxide/carbon (ATO/C) carrier, which has a uniform size, is uniformly dispersed and is hardly agglomerated, by taking ammonia water or sodium hydroxide solution as a precipitator; dissolving H2PtCl6.6H2O in ethylene glycol; heating in the atmosphere of inert gas to obtain platinum (Pt) sol; and mixing the PT sol and ethanol suspension of the ATO/C to obtain a Pt-ATO/C catalyst. The method has a simple process and is low in cost; and the prepared Pt-ATO/C catalyst is highly dispersed, has a uniform particle size, does not have obvious agglomeration phenomenon, has a lower starting potential and higher current density, shows excellent electro-catalytic oxidation activity and efficiency and has ...

Umbrella shaped memory alloy support in femoral head

An umbrella shaped memory alloy support in femoral head comprises an umbrella shaped support and a support sleeve, wherein the lower end of the umbrella shaped support is in sleeve joint with the support sleeve by an umbrella handle; the umbrella shaped support is formed by eight supporting ribs; the support sleeve is formed by rolling plates; blood supply holes are distributed on the wall of the support sleeve; and withdrawal preventing pawls are arranged on the outer surface of the support sleeve. The support not only plays a powerful supporting role in femoral head, but also can assist in revascularization of femoral head, prevent further collapse of femoral head, alleviate more pains of the patients, shorten the operation time and simplify the operation, is safe and reliable in supporting and has obvious effect on treating collapse of femoral head caused by adult avascular necrosis of femoral head. The support has simple process and shape, is easy to deform and insert, is stable in ...

Method for identifying Tan sheep based on SNP (Single Nucleotide Polymorphism) site combination

The invention discloses a method for identifying Tan sheep based on SNP (Single Nucleotide Polymorphism) site combination. The method comprises the following steps: detecting nucleotide at each SNP site in a specific SNP site combination in a genome of a to-be-detected sheep, and determining a genotype of the to-be-detected sheep corresponding to the specific SNP site combination; determining whether the to-be-detected sheep is the Tan sheep according to the following steps: if the genotype of the to-be-detected sheep corresponding to the specific SNP site combination conforms to the standard genotype of the Tan sheep, determining that the to-be-detected sheep is the Tan sheep or a candidate Tan sheep; if the genotype, corresponding to the specific SNP site combination, of the to-be-detected sheep conforms to the standard genotype of the non-Tan sheep, the to-be-detected sheep is the non-Tan sheep or the candidate to be the non-Tan sheep. The SNP locus combination for identifying the Tan ...

Method for detecting goat fertility

The invention discloses a method for detecting the productive capacity of a goat. The method is to detect the 757 bit nucleic acid of a GnRHR gene in the gene group of the detected goat to be G or be mutated into A and confirms the genotype of the goat, then confirms the productive capacity of the goat by the genotype; if the 757 bit nucleic acid of the GnRHR gene in the gene group of the detected goat is G, the genotype of the homozygote thereof is AA; when the 757 bit nucleic acid of the GnRHR gene in the gene group of the detected goat is A, the genotype of the homozygote thereof is BB; the genotype of the heterozygous thereof is AB; the productive capacity of the goat of BB genotype is better than that of the goats with AB genotype and AA genotype. The method of the invention utilizes inheritance to mark the polymorphism for confirming the productive capacity of the goat, can fast and conveniently confirm the productive capacity of the goat of Jining and provides an accurate and simple ...

SINGLE NUCLEOTIDE POLYMORPHISM MARKER RELATED TO CHINESE HORSE SHORT STATURE TRAIT AND USE THEREOF

The invention relates to a single nucleotide polymorphism (SNP) marker related to a Chinese horse short stature trait. The SNP molecular marker is located at the 501position of a sequence shown in SEQ ID NO.1, polymorphism is G/A, and the SNP marker corresponds to base pair 18,205,998 on chromosome 8 in a horse. The SNP marker related to the Chinese horse short stature trait and use thereof provided by the present invention have the following advantages that: (1) the molecular marker is not restricted by the age, sex and the like of Chinese horses, is used in early breeding of the Chinese horses, performs accurate screening immediately at birth, and significantly promotes the breeding process of dominant pony varieties of the Chinese horse; (2) a method for detecting SNP of a Chinese horse TBX3 gene is accurate, reliable, and easy to operate. 110.-. (canceled)12. A kit or reagent comprising the specific primer combination according to .13. A method for using the specific primer combination according to or the kit or reagent according to in identifying pony varieties of Chinese horse claim 11 , molecular marker-assisted breeding of Chinese horses claim 11 , or improving germplasm resources of Chinese horses.14. The method according to claim 13 , wherein a method for identifying pony varieties of Chinese horse comprises the following steps:1) extracting genomic DNA from a test horse;2) using the genomic DNA of the test horse as a template to conduct polymerase chain reaction (PCR) amplification with specific primers, to obtain a fragment of an amplified product; and3) determining what type of a base is located at 501 bp in the fragment of the amplified product: if the base is A, then the test horse is determined to have a short stature trait; if the base is G, the test horse is determined to have a tall stature trait; namely, in the detection, an idiotype with fluorescence of primer 1 labeled groups is AA, and an idiotype with fluorescence of primer 2 labeled groups ...

Integration slip casting management device

The utility model discloses an integration slip casting management device, including an electric -magnetic flow meter, a pressure sensor, the 2nd electric -magnetic flow meter, the 2nd pressure sensor and data acquisition and administrative unit. The utility model relates to an integration slip casting management device only needs during the use directly to link the first slip casting pump body, the second slip casting pump body the first feed inlet of slip casting management device, the second feed inlet can. Its characteristics are data acquisition and administrative unit, an electric -magnetic flow meter, a pressure sensor, the 2nd electric -magnetic flow meter, the 2nd pressure sensor integrate in the box to collection, record and the management of slip casting data are accomplished at the incasement. The utility model discloses an integrated design is compared with current slip casting record appearance, need not additionally to purchase flowmeter and pressure sensor again, reduces ...

Deamination tower suitable for MVR evaporimeter flash steam

The utility model provides a deamination tower suitable for MVR evaporimeter flash steam, including deamination tower body, harrow formula distributor and acid circulating pump, harrow formula distributor is located that the deamination tower is inside to link to each other with the air inlet, and the even air vent of distributor trunk and branch bottom, acid circulating pump are located the subaerial of deamination tower next door, deamination tower body bottom is equipped with sour export, and well lower part is equipped with air inlet, level gauge liquid phase interface and adding acid mouth, and the middle part is equipped with recycle acid import, online pH meter, hand hole and absorption liquid export, and well upper portion is equipped with silk screen demister, manhole and silk screen washing water inlet, and the top is equipped with gas outlet and level gauge gaseous phase interface. The utility model discloses both guaranteed that the gas scrubbing effect has reduced the inner ...

Memory alloy intelligent swirl diffuser

A memory alloy intelligent swirl diffuser comprises a barrel. A cross beam is arranged in the barrel. A central shaft is arranged on the cross beam, penetrates through a fixed blade and is fixed to a movable blade. A memory alloy intelligent controller is arranged on the cross beam and is mounted at the other end of the central shaft in a sleeved mode. The memory alloy intelligent controller of the memory alloy temperature-sensing element in the structure of the memory alloy intelligent swirl diffuser is a passive intelligent element. The central shaft and the movable blade are driven to move through power generated according to the deformation characteristic of a memory alloy. The gap between the fixed blade and the movable blade is adjusted to change the wind direction of the swirl diffuser, control over the air flow direction and air flow is achieved through the memory alloy intelligent swirl diffuser, the purpose of temperature adjustment is achieved, and the trouble of manual or power-driven ...

Modular surround coaptation ware

The utility model provides a modular surround coaptation ware, includes two semicircle coaptation boards, the coaptation board includes the arm of surrouning of coaptation board crossbeam and coaptation board crossbeam below, two coaptation board crossbeam adopts sawtooth combination or other combining form, two and half coaptation board crossbeams are through the fastening of a plurality of fixed plates. The utility model discloses modular surround coaptation ware has the implantation and takes out easily, shortens operation time, reduces the characteristics that the operation is difficult. Through the fixed plate be connected with two semicircle coaptation boards because conveniently taking out of postsurgical has been realized to self structural feature when having realized fastening coaptation board. Simultaneously can be according to a plurality of fixed plates of the constant -length of coaptation board to can have many well specifications, can handle different tubulose bone fracture ...

Method for identifying Tan sheep and non-Tan sheep based on genotyping

The invention discloses a method for identifying Tan sheep and non-Tan sheep based on genotyping. The method comprises the following steps: detecting nucleotide at each SNP site in a specific SNP site combination in a genome of a to-be-detected sheep, and determining a genotype of the to-be-detected sheep corresponding to the specific SNP site combination; determining whether the to-be-detected sheep is the Tan sheep according to the following steps: if the genotype of the to-be-detected sheep corresponding to the specific SNP site combination conforms to the standard genotype of the Tan sheep, determining that the to-be-detected sheep is the Tan sheep or a candidate Tan sheep; if the genotype, corresponding to the specific SNP site combination, of the to-be-detected sheep conforms to the standard genotype of the non-Tan sheep, the to-be-detected sheep is the non-Tan sheep or the candidate to be the non-Tan sheep. The SNP locus combination for identifying the Tan sheep and the non-Tan sheep ...

Snp marker related to wool traits of fine-wool sheep, and detection primer set, kit, detection method and use thereof

Disclosed is an SNP marker related to wool traits of a fine-wool sheep, and a detection primer set, a detection method and use thereof, which belong to the technical field of molecular marker detection; the SNP marker is located at 133486008bp on sheep chromosome 3. There is an A/C base mutation at the site, which is significantly related to the wool traits of fine-wool sheep. The SNP marker of the present disclosure may be used in molecular marker assisted breeding of sheep. The molecular marker provided by the present disclosure is not limited by the age, sex, etc. of the sheep, may be used for fine-wool sheep breeding (even a sheep at birth may be accurately screened), and significantly promotes the breeding process of fine-wool sheep.