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09-05-2017 дата публикации

Method for improving analysis of microorganisms in complex matrices

Номер: US0009645057B2
Автор: Xiao Li, Kenneth Anthony Kopher, John D. Mantlo, William Alfred Pope, Song Shi, Axel A. Yup, LI XIAO, KOPHER KENNETH ANTHONY, MANTLO JOHN D, POPE WILLIAM ALFRED, SHI SONG, YUP AXEL A, Li Xiao, Kopher Kenneth Anthony, Mantlo John D., Pope William Alfred, Shi Song, Yup Axel A.
Принадлежит: Becton, Dickiinson and Company, BECTON DICKINSON CO, BECTON DICKIINSON AND COMPANY, Becton, Dickinson and Company

A process for determining one of the presence, absence, or total of microorganisms (e.g. bacteria) in a sample. According to the process, a biological sample containing complex matrices is obtained. The sample is first combined with a resin to adsorb complex matrices from the sample. The resin is removed from the biological sample. The sample so prepared is then analyzed by flow cytometry.

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Номер записи: 1
03-04-2018 дата публикации

Sample preparation for flow cytometry

Номер: US0009933341B2
Автор: Xiao Li, Yongqiang Zhang, Kenneth Anthony Kopher, John D. Mantlo, William Alfred Pope, Song Shi, Axel A. Yup, LI XIAO, ZHANG YONGQIANG, KOPHER KENNETH ANTHONY, MANTLO JOHN D, POPE WILLIAM ALFRED, SHI SONG, YUP AXEL A, Li Xiao, Zhang Yongqiang, Kopher Kenneth Anthony, Mantlo John D., Pope William Alfred, Shi Song, Yup Axel A.
Принадлежит: Becton, Dickinson and Company, BECTON DICKINSON CO

Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.

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Номер записи: 2
25-04-2017 дата публикации

Identification of microorganisms using MALDI-TOF-MS on-plate extraction

Номер: US0009631221B1
Автор: Liping Feng, William B. Brasso, Susan M. Kircher, Vanda White, Song Shi, Xiao Mo, Tuan-Linh Ngoc Nguyen, Adrien Malick, Jon E. Salomon, John D. Mantlo, FENG LIPING, BRASSO WILLIAM B, KIRCHER SUSAN M, WHITE VANDA, SHI SONG, MO XIAO, NGUYEN TUAN-LINH NGOC, MALICK ADRIEN, SALOMON JON E, MANTLO JOHN D, Feng Liping, Brasso William B., Kircher Susan M., White Vanda, Shi Song, Mo Xiao, Nguyen Tuan-Linh Ngoc, Malick Adrien, Salomon Jon E., Mantlo John D.
Принадлежит: Becton, Dickinson and Company, FENG LIPING, BRASSO WILLIAM B, KIRCHER SUSAN M, WHITE VANDA, SHI SONG, MO XIAO, NGUYEN TUAN-LINH NGOC, MALICK ADRIEN, SALOMON JON E, MANTLO JOHN D, BECTON DICKINSON CO, Feng Liping, Brasso William B., Kircher Susan M., White Vanda, Shi Song, Mo Xiao, Nguyen Tuan-Linh Ngoc, Malick Adrien, Salomon Jon E., Mantlo John D.

Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

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Номер записи: 3
17-08-2017 дата публикации

METHOD OF SAMPLE PREPARATION FOR MALDI AND AUTOMATED SYSTEM THEREFOR

Номер: US20170234780A1
Автор: Timothy Wiles, John D. Mantlo, WILES TIMOTHY, MANTLO JOHN D, Wiles Timothy, Mantlo John D.
Принадлежит: Becton, Dickinson and Company

Methods for preparing a biological sample for testing by Maldi where such methods are selected based on sample parameters. Maldi scores are obtained for a range of sample parameters (e.g. McFarland, dispense volume and number of dispenses). From the data, sample preparation parameters can be selected for a biological sample being prepared for Maldi testing. One sample preparation strategy uses multiple dispenses of sample with an intervening drying step, which yields more accurate Maldi scores, particularly for samples at the low range of McFarland values (e.g. below about 2). 1. A method for preparing a biological sample for evaluation by Matrix-Assisted Laser Desorption Ionization Time of Flight mass spectrometry the method comprising:obtaining a biological sample;diluting the sample;applying a first aliquot of the diluted sample to a surface adapted to deliver a sample to a device configured to perform mass spectrometry;drying the sample;applying a second aliquot of the sample over the first, dried aliquot;drying the sample;applying a matrix over the dried sample, the matrix adapted for use in the mass spectrometer; andperforming mass spectrometry on the sample.2. The method of wherein the sample is diluted by combining the sample with a sterile diluent.3. The method of wherein the biological sample is a portion of a colony of a microorganism picked from a culture plate.4. The method of wherein the biological sample is collected from a positive blood culture.5. The method of wherein the surface is a plate adapted for use in a mass spectrometer.6. The method of further comprising measuring the turbidity of the diluted sample wherein the diluted sample has a turbidity of about 1 to about 2 McFarland claim 1 , the method further comprising applying a first aliquot of about 3 μl on the surface claim 1 , drying the sample and applying a second aliquot of about 3 μl on the surface.7. The method of wherein the matrix solution is selected from the group consisting of α- ...

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Номер записи: 4
20-07-2017 дата публикации

IDENTIFICATION OF MICROORGANISMS USING MALDI-TOF-MS ON-PLATE EXTRACTION

Номер: US20170204448A1
Автор: Liping Feng, William B. Brasso, Susan M. Kircher, Vanda White, Song Shi, Xiao Mo, Tuan-Linh Ngoc Nguyen, Adrien P. Malick, Jon E. Salomon, John D. Mantlo, Mary R. Votta, Ben Turng, Donald R. Callihan, Wendy Louise Williams, FENG LIPING, BRASSO WILLIAM B, KIRCHER SUSAN M, WHITE VANDA, SHI SONG, MO XIAO, NGUYEN TUAN-LINH NGOC, MALICK ADRIEN P, SALOMON JON E, MANTLO JOHN D, VOTTA MARY R, TURNG BEN, CALLIHAN DONALD R, WILLIAMS WENDY LOUISE, Feng Liping, Brasso William B., Kircher Susan M., White Vanda, Shi Song, Mo Xiao, Nguyen Tuan-Linh Ngoc, Malick Adrien P., Salomon Jon E., Mantlo John D., Votta Mary R., Turng Ben, Callihan Donald R., Williams Wendy Louise
Принадлежит:

Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes. 1. A method for characterizing at least one microorganism in a sample for identification of microorganisms therein , the method comprising:(a) obtaining a sample containing at least one microorganism;(b) depositing at least a portion of the sample on a solid surface adapted to be placed in an apparatus configured to determine the identity of microorganisms by mass spectrometry;(c) optionally, drying the sample;(d) treating the sample with a solution selected from the group consisting of a volatile acid solution, an organic solvent, a combination of organic solvent and volatile acid solution, and combinations thereof, wherein the volume percent of the volatile acid solution combined with the sample is at least about 50%;(e) optionally, drying the sample;(f) placing a matrix over the treated sample;(g) optionally, drying the sample; and(h) identifying the microorganisms by a mass spectrometry.2. The method of claim 1 , wherein the sample obtained is a microbial pellet that is deposited by direct smear.3. The method of claim 1 , wherein the sample obtained is a microbial suspension in a solution selected from the group consisting of water claim 1 , an organic solvent claim 1 , and combinations thereof.4. The method of claim 1 , wherein the ...

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Номер записи: 5
14-01-2016 дата публикации

A METHOD FOR IMPROVING ANALYSIS OF MICROORGANISMS IN COMPLEX MATRICES

Номер: US20160011087A1
Автор: Kopher Kenneth Anthony, Li Xiao, Mantlo John D., Pope William Alfred, Shi Song, Yup Axel A.
Принадлежит: BECTON, DICKINSON AND COMPANY

A process for determining one of the presence, absence, or total of microorganisms (e.g. bacteria) in a sample. According to the process, a biological sample containing complex matrices is obtained. The sample is first combined with a resin to adsorb complex matrices from the sample. The resin is removed from the biological sample. The sample so prepared is then analyzed by flow cytometry. 1. A method of analyzing a biological or environmental sample for the presence or absence of at least one target microorganism , comprising:obtaining a sample to be tested for the presence or absence of the at least one target microorganism;preparing the sample;combining the sample with a resin selected to bind to non-target particles in the sample;removing the resin from the sample, the resin carrying the non-target particles therewith;combining the sample with a nucleic acids stain after removing the resin therefrom, wherein the nucleic acid stain permeates and labels the target microorgansim; andanalyzing the prepared sample.2. The method of wherein the biological or environmental sample is one derived from a complex matrix.3. The method of wherein the complex matrix is selected from the group consisting of food products claim 1 , cosmetics claim 1 , and soil samples.4. The method of wherein the food product is selected from the group consisting of meat claim 3 , milk claim 3 , eggs claim 3 , butter claim 3 , and water.5. The method of wherein the resin is selected from a group consisting of hydrophobic resin claim 1 , a polyaromatic resin claim 1 , and a porous resin.6. The method of wherein the resin is a non-functional polymeric resin adsorbent.7. The method of wherein the sample and resin are incubated at room temperature.8. The method of wherein the nucleic acid stain label is a fluorescent label.9. The method of wherein the sample is analyzed using a flow cytometer.10. The method of further comprising adding a quencher to the biological sample claim 1 , wherein the ...

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Номер записи: 6
12-03-2020 дата публикации

METHOD OF SAMPLE PREPARATION FOR MALDI AND AUTOMATED SYSTEM THEREFOR

Номер: US20200080919A1
Автор: Mantlo John D., Wiles Timothy
Принадлежит:

Methods for preparing a biological sample for testing by Maldi where such methods are selected based on sample parameters. Maldi scores are obtained for a range of sample parameters (e.g. McFarland, dispense volume and number of dispenses). From the data, sample preparation parameters can be selected for a biological sample being prepared for Maldi testing. One sample preparation strategy uses multiple dispenses of sample with an intervening drying step, which yields more accurate Maldi scores, particularly for samples at the low range of McFarland values (e.g. below about 2). 1. A method for preparing a biological sample for evaluation by Matrix-Assisted Laser Desorption Ionization Time of Flight mass spectrometry the method comprising:obtaining a biological sample;diluting the biological sample;measuring a concentration of the biological sample;if the measured biological sample concentration is within a predetermined range, applying a first predetermined aliquot of the biological sample to a surface adapted to deliver the applied biological sample to a device configured to perform mass spectrometry;if a measured biological sample concentration is higher than the predetermined range, applying a second predetermined aliquot of the biological sample to the surface adapted to deliver the applied biological sample to the device configured to perform mass spectrometry, wherein the second predetermined aliquot is less than the first predetermined aliquot; i) applying a first aliquot of the biological sample to the surface adapted to deliver the biological sample to the device configured to perform mass spectrometry;', 'ii) drying the sample;', 'iii) applying a second aliquot of the biological sample over the dried sample from the first aliquot;', 'iv) drying the biological sample;, 'if a measured biological sample concentration is lower than the predetermined range,'}applying a matrix solution over the applied biological sample, the matrix solution being adapted for use ...

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Номер записи: 7
19-03-2020 дата публикации

FORMULATIONS AND PROCESS FOR ISOLATING VIABLE MICROORGANISMS FROM POSITIVE BLOOD CULTURES

Номер: US20200087702A1
Автор: Beaty Patrick Shawn, Brasso William B., Bruton Jeffery H., Callihan Donald R., Douglass John P., Feng Liping, Gosnell Curtis M., Kircher Susan M., Luper Dyan, Malick Adrien P., Mantlo John D., Rosales Julie L., Turng Ben, White Vanda, Zhou James Y.
Принадлежит: BECTON DICKINSON AND COMPANY

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including , from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism(s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods. 132-. (canceled)34. The method of claim 33 , wherein the portion of the positive blood culture sample is mixed with an equal volume of buffer to produce the mixture.35. The method of claim 33 , further comprising resuspending the second pellet in a solution and performing at least one downstream testing of the resuspended second pellet selected from the group consisting of identification of the microorganisms by mass spectrometry claim 33 , phenotypic identification claim 33 , antimicrobial susceptibility testing claim 33 , and molecular testing.36. The method of claim 33 , wherein the microorganisms are selected from the group consisting of gram-positive bacteria claim 33 , gram-negative bacteria claim 33 , and yeast.37Streptococcus pneumoniae.. The method of claim 36 , wherein the gram-positive bacteria is38. The method of wherein the nutrient base solution consists of casein peptone at a concentration in the buffer of about 8 g/L to about 35 g/L claim 33 , sodium chloride at a concentration in the buffer of about 2 g/L to about 10 g/L claim 33 , soy peptone at a concentration in the buffer of about 1.5 g/L to about 15 g/L claim 33 , and potassium phosphate at a concentration in the buffer of about 0.5 g/L to about 5 g/L and optionally at least one other nutrient ...

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Номер записи: 8
02-06-2022 дата публикации

IDENTIFICATION OF MICROORGANISMS USING MALDI-TOF-MS ON-PLATE EXTRACTION

Номер: US20220170065A1
Автор: Brasso William B., Feng Liping, Kircher Susan M., Malick Adrien P., Mantlo John D., Mo Xiao, Nguyen Tuan-Linh Ngoc, Salomon Jon E., Shi Song, White Vanda
Принадлежит: BECTON DICKINSON AND COMPANY

Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes. 1. A kit for preparing a positive blood culture sample for identification of microorganisms therein , the kit comprising:a lysis buffer, wherein the lysis buffer is selected to lyse blood cells in the sample while microorganisms, if any, remain intact;a volatile acid solution, wherein a volume percent of the volatile acid is at least 70% of the volatile acid solution; anda matrix.2. The kit of claim 1 , wherein the volatile acid is selected from the group consisting of formic acid claim 1 , acetic acid claim 1 , trifluoracetic acid and hydrochloric acid.3. The kit of claim 1 , further comprising an organic solvent claim 1 , wherein the organic solvent is selected from the group consisting of ethanol claim 1 , methanol claim 1 , isopropanol claim 1 , or acetone.4. The kit of claim 1 , wherein the kit comprises a volatile acid solution that is 70% volatile acid.5. The kit of claim 3 , wherein the organic solvent is a solution is 100% organic solvent.6. The kit of claim 3 , wherein the organic solvent is a solution is 30% organic solvent to about 70% organic solvent.7. The kit of claim 1 , where the kit further comprises a fixative claim 1 , wherein the fixative is selected from the group consisting of an organic solvent and a fixative.8. The kit ...

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Номер записи: 9
30-04-2015 дата публикации

SAMPLE PREPARATION FOR FLOW CYTOMETRY

Номер: US20150118677A1
Автор: Kopher Kenneth Anthony, Li Xiao, Mantlo John D., Pope William Alfred, Shi Song, Yup Axel A., Zhang Yongqiang
Принадлежит: BECTON, DICKINSON AND COMPANY

Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain. 1. A method of analyzing a sample for the amount of viable microorganisms , comprising:obtaining a sample to be tested for the presence or absence of the at least one target microorganism;preparing the sample;adding a background signal-reducing substance comprising an excess amount of background signal-reducing molecules;adding a nucleic acid stain that permeates and labels target viable cells of the microorganism;andanalyzing the prepared sample,wherein the background signal-reducing molecules bind to non-target particles in a similar manner and a with a similar efficiency as the nucleic acid stain.2. The method of claim 1 , wherein the sample is selected from the group consisting of a food sample claim 1 , an environmental sample claim 1 , a cosmetic sample claim 1 , and a biological sample.3. The method of claim 1 , wherein the concentration of the background signal-reducing molecules is 0.1 μM to 50 μM when combined with the sample.4. The method of claim 1 , wherein the concentration of the background signal-reducing molecules is 0.1 μM to 10 μM when combined with the sample.5. The method of claim 1 , wherein the concentration of the background signal-reducing molecules is 0.5 μM to 5 μM when combined with the sample.6. The method of claim 1 , wherein the background signal-reducing molecules are incubated with the sample for 2 minutes to 1 hour prior to the addition of the nucleic acid stain.7. The method of claim 1 , wherein the background signal-reducing ...

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Номер записи: 10
07-05-2015 дата публикации

FORMULATIONS AND PROCESS FOR ISOLATING VIABLE MICROORGANISM FROM POSITIVE BLOOD CULTURES

Номер: US20150125895A1
Автор: Beaty Patrick Shawn, Brasso William B., Bruton Jeffery H., Callihan Donald R., Douglass John P., Feng Liping, Gosnell Curtis M., Kircher Susan M., Luper Dyan, Malick Adrien P., Mantlo John D., Rosales Julie L., Turng Ben, White Vanda, Zhou James Y.
Принадлежит: BECTON, DICKINSON AND COMPANY

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including , from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods. 1S. pneumoniae.. A buffer for isolating and concentrating viable microorganism from a positive blood culture sample comprising a base solution; at least one non-ionic detergent; at least one thiol; and optionally , ammonium chloride , wherein the relative amounts of the base solution , the at least one non-ionic detergent , and the at least one thiol in the buffer are selected to preserve the viability of2. The buffer of claim 1 , wherein the at least one non-ionic detergent comprises triton X-100 at a concentration in the buffer of up to about 1 g/L.3. The buffer of claim 1 , wherein the at least one non-ionic detergent comprises triton X-100 at a concentration in the buffer of about 0.335 g/L.4. The buffer of claim 1 , wherein the base solution comprises at least one selected from the group consisting of a nutrient broth claim 1 , an isotonic buffer claim 1 , a peptone claim 1 , and a salt.5. The buffer of claim 4 , wherein concentration of the nutrient broth in the buffer is about 10 g/L to about 50 g/L.6. The buffer of claim 4 , wherein the nutrient broth comprises trypticase soy broth.7. The buffer of claim 4 , wherein the isotonic buffer comprises at least one selected from the group consisting of sodium phosphate claim 4 , potassium phosphate claim 4 , ...

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Номер записи: 11
14-05-2020 дата публикации

IDENTIFICATION OF MICROORGANISMS USING MALDI-TOF-MS ON-PLATE EXTRACTION

Номер: US20200149085A1
Автор: Brasso William B., Feng Liping, Kircher Susan M., Malick Adrien P., Mantlo John D., Mo Xiao, Nguyen Tuan-Linh Ngoc, Salomon Jon E., Shi Song, White Vanda
Принадлежит:

Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes. 120-. (canceled)21. A method for characterizing at least one microorganism in a sample for identification of microorganisms therein , the method comprising:(a) obtaining a sample containing at least one microorganism wherein the sample is deposited by direct smear;(b) depositing at least a portion of the obtained sample on a solid surface adapted to be placed in an apparatus configured to determine the identity of microorganisms by mass spectrometry;(c) drying the deposited sample;(d) treating the deposited and dried sample with a volatile acid solution, wherein the volume percent of the volatile acid is at least 70% of the volatile acid solution, and the volatile acid solution is a volatile acid in water or in an organic solvent;(e) drying the treated sample;(f) placing a matrix over the treated and dried sample;(g) drying the matrix-covered sample; and(h) identifying the microorganisms by a mass spectrometry.22. The method of claim 21 , wherein the volatile acid solution is formic acid in water at a volume percent of 70%.23. The method of claim 21 , wherein the volatile acid solution is formic acid in water at a volume percent of about 80%.24. The method of claim 21 , wherein the volatile acid solution is formic acid in water at a volume ...

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Номер записи: 12
30-08-2018 дата публикации

UNIQUE SAMPLE TRANSFER DEVICE FOR AN AUTOMATED PIPETTOR FOR PROCESSING A VARIETY OF CLINICAL MICROBIOLOGICAL SPECIMENS

Номер: US20180243735A1
Автор: Mantlo John D., Zhou Tong
Принадлежит: BECTON DICKINSON AND COMPANY

The present disclosure describes pipette tips haying an absorbent material (e.g., a flocking material) anchored to a distal end of the pipette tip and related methods of use. In some embodiments, the flocked pipette tips can be used in an automated process and/or system, wherein the contact between the pipette tips and either a sample or a culture medium can be automatically sensed for accurate sample collection and/or dispense. In some embodiments, automatic detection of the liquid interface may be accomplished by detecting a threshold change in capacitance when the pipette tip contacts the sample liquid interface (e.g., for sample collection) or the agar interface (e.g., for sample release). In some embodiments, the automated process and/or system may utilize one or more predetermined, fixed heights for collecting samples and/or depositing samples. 1. A pipette tip comprising:an elongated body having a proximal end and a distal end;an interface at the proximal end of the elongated body for engaging a robotic pipettor; andflocking material anchored to the distal end of the elongated body.2. The pipette tip of claim 1 , wherein the flocking material comprises hydrophilic fibers.3. The pipette tip of claim 2 , wherein the flocking material comprises cotton.4. The pipette tip of claim 2 , wherein the flocking material is anchored to the distal end of the pipette tip through the use of an adhesive.5. The pipette tip of claim 2 , wherein the elongated body is constructed of a conductive material.6. A system comprising:a robotic pipettor having a capacitance sensor, wherein the robotic pipettor is configured to hold one or more pipette tips;a conductive pipette tip having an absorbent material anchored to a distal end of the conductive pipette tip; anda capacitance detecting circuit configured to detect contact between the conductive pipette tip when a signal from the capacitance sensor exceeds a predetermined threshold.76. The system claim 2 , wherein the absorbent ...

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Номер записи: 13
17-11-2022 дата публикации

METHOD OF SAMPLE PREPARATION FOR MALDI AND AUTOMATED SYSTEM THEREFOR

Номер: US20220364965A1
Автор: Mantlo John D., Wiles Timothy
Принадлежит: BECTON, DICKINSON AND COMPANY

Methods for preparing a biological sample for testing by Maldi where such methods are selected based on sample parameters. Maldi scores are obtained for a range of sample parameters (e.g. McFarland, dispense volume and number of dispenses). From the data, sample preparation parameters can be selected for a biological sample being prepared for Maldi testing. One sample preparation strategy uses multiple dispenses of sample with an intervening drying step, which yields more accurate Maldi scores, particularly for samples at the low range of McFarland values (e.g. below about 2).

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Номер записи: 14
05-04-2022 дата публикации

Unique sample transfer device for an automated pipettor for processing a variety of clinical microbiological specimens

Номер: US11291986B2
Автор: John D. Mantlo, TONG Zhou
Принадлежит: Becton Dickinson and Co

The present disclosure describes pipette tips having an absorbent material (e.g., a flocking material) anchored to a distal end of the pipette tip and related methods of use. In some embodiments, the flocked pipette tips can be used in an automated process and/or system, wherein the contact between the pipette tips and either a sample or a culture medium can be automatically sensed for accurate sample collection and/or dispense. In some embodiments, automatic detection of the liquid interface may be accomplished by detecting a threshold change in capacitance when the pipette tip contacts the sample liquid interface (e.g., for sample collection) or the agar interface (e.g., for sample release). In some embodiments, the automated process and/or system may utilize one or more predetermined, fixed heights for collecting samples and/or depositing samples.

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Номер записи: 15
10-10-2013 дата публикации

Sample preparation for flow cytometry

Номер: CA2869472A1
Автор: Axel A. Yup, John D. Mantlo, Kenneth Anthony Kopher, Song Shi, William Alfred Pope, XIAO Li, Yongqiang Zhang
Принадлежит: Becton Dickinson and Co

Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.

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Номер записи: 16
18-09-2014 дата публикации

Formulations and process for isolating viable microorganism from positive blood cultures

Номер: AU2013225974A1
Автор: Adrien P. Malick, Ben Turng, Curtis M. Gosnell, Donald R. Callihan, Dyan Luper, James Y. Zhou, Jeffery H. Bruton, John D. Mantlo, John P. Douglass, Julie L. Rosales, Liping Feng, Shawn Beaty, Susan M. Kircher, Vanda White, William B. Brasso
Принадлежит: Becton Dickinson and Co

Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism ( s ) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

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Номер записи: 17
25-02-2016 дата публикации

Method of sample preparation for maldi and automated system therefor

Номер: CA2957928A1
Автор: John D. Mantlo, Timothy Wiles
Принадлежит: Becton Dickinson and Co

Methods for preparing a biological sample for testing by Maldi where such methods are selected based on sample parameters. Maldi scores are obtained for a range of sample parameters (e.g. McFarland, dispense volume and number of dispenses). From the data, sample preparation parameters can be selected for a biological sample being prepared for Maldi testing. One sample preparation strategy uses multiple dispenses of sample with an intervening drying step, which yields more accurate Maldi scores, particularly for samples at the low range of McFarland values (e.g. below about 2).

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Номер записи: 18
24-03-2020 дата публикации

Method of sample preparation for maldi and automated system therefor

Номер: CA2957928C
Автор: John D. Mantlo, Timothy Wiles
Принадлежит: Becton Dickinson and Co

Methods for preparing a biological sample for testing by Maldi where such methods are selected based on sample parameters. Maldi scores are obtained for a range of sample parameters (e.g. McFarland, dispense volume and number of dispenses). From the data, sample preparation parameters can be selected for a biological sample being prepared for Maldi testing. One sample preparation strategy uses multiple dispenses of sample with an intervening drying step, which yields more accurate Maldi scores, particularly for samples at the low range of McFarland values (e.g. below about 2).

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Номер записи: 19
18-09-2014 дата публикации

A method for improving analysis of microorganisms in complex matrices

Номер: CA2905651A1
Автор: Axel A. Yup, John D. Mantlo, Kenneth Anthony Kopher, Song Shi, William Alfred Pope, XIAO Li
Принадлежит: Becton Dickinson and Co

A process for determining one of the presence, absence, or total of microorganisms (e.g. bacteria) in a sample. According to the process, a biological sample containing complex matrices is obtained. The sample is first combined with a resin to adsorb complex matrices from the sample. The resin is removed from the biological sample. The sample so prepared is then analyzed by flow cytometry.

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Номер записи: 20
03-10-2023 дата публикации

Method of sample preparation for maldi and automated system therefor

Номер: US11774332B2
Автор: John D. Mantlo, Timothy Wiles
Принадлежит: Becton Dickinson and Co

Methods for preparing a biological sample for testing by Maldi where such methods are selected based on sample parameters. Maldi scores are obtained for a range of sample parameters (e.g. McFarland, dispense volume and number of dispenses). From the data, sample preparation parameters can be selected for a biological sample being prepared for Maldi testing. One sample preparation strategy uses multiple dispenses of sample with an intervening drying step, which yields more accurate Maldi scores, particularly for samples at the low range of McFarland values (e.g. below about 2).

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Номер записи: 21
14-03-2024 дата публикации

Method of sample preparation for maldi and automated system therefor

Номер: US20240085285A1
Автор: John D. Mantlo, Timothy Wiles
Принадлежит: Becton Dickinson and Co

Methods for preparing a biological sample for testing by Maldi where such methods are selected based on sample parameters. Maldi scores are obtained for a range of sample parameters (e.g. McFarland, dispense volume and number of dispenses). From the data, sample preparation parameters can be selected for a biological sample being prepared for Maldi testing. One sample preparation strategy uses multiple dispenses of sample with an intervening drying step, which yields more accurate Maldi scores, particularly for samples at the low range of McFarland values (e.g. below about 2).

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Номер записи: 22
07-12-2021 дата публикации

Identification of microorganisms using MALDI-TOF-MS on-plate extraction

Номер: US11193158B2
Автор: Adrien P. Malick, John D. Mantlo, Jon E. Salomon, Liping Feng, Song Shi, Susan M. Kircher, Tuan-Linh Ngoc Nguyen, Vanda White, William B. Brasso, Xiao Mo
Принадлежит: Becton Dickinson and Co

Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

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Номер записи: 23
11-02-2020 дата публикации

Identification of microorganisms using MALDI-TOF-MS on-plate extraction

Номер: US10557162B2
Автор: Adrien P. Malick, John D. Mantlo, Jon E. Salomon, Liping Feng, Song Shi, Susan M. Kircher, Tuan-Linh Ngoc Nguyen, Vanda White, William B. Brasso, Xiao Mo
Принадлежит: Becton Dickinson and Co

Rapid methods that identify sepsis-causing bacteria or yeast aid the physician in critical therapeutic decision-making, thus decreasing patient mortality rates. The methods described herein employ plating microorganisms directly on to a MALDI-MS plate, adding concentrated formic acid, and identifying the microorganism by mass spectrometry. Optionally, an organic solvent may be combined with the formic acid, or added to the sample before or after the concentrated formic acid is added thereto. The methods enable direct extraction of proteins from microorganisms without the need for liquid protein extraction methods and yields positive identification results for gram-positive bacteria, gram-negative bacteria and yeast in minutes.

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Номер записи: 24