09-05-2013 дата публикации
Номер: US20130116344A1
Принадлежит:
The present invention relates to the use of at least one nucleic acid comprising or consisting of: 3Ascochyta phleina, Curvularia affinis, Glomerella graminicola, Thanatephorus cucumeris, Pythium ultimum, Gaeumannomyces graminis, Marasmius oreades, Corticium fuciforme, Phytophthora nicotianae. Fusarium culmorum, Bipolaris sorokiniana, Microdochium nivale, Rhizoctonia cerealis, Pythium graminicola, Rhynchosporium secalis, Sclerotinia homoeocarpa, Typhula incarnate, Ustilago striiformis or Septoria macropoda.. The method according to claim 1 , wherein the fungus is4. The method according to claim 1 , wherein the sample is a soil sample taken from which the turfgrass is grown.5. The method according to claim 1 , wherein the sample is a turfgrass sample.6. The method according to claim 5 , wherein the turfgrass sample is a root sample.7. The method according to claim 1 , wherein the turfgrass is Festaceae claim 1 , Aveneae claim 1 , Triticeae claim 1 , Chlorideae claim 1 , Zoysieae claim 1 , Paniceae or Andropogoneae Tribe.8. The method according to claim 1 , wherein the nucleic acid amplification is Amplification Fragment Length Polymorphism (AFLP) or Terminal Restriction Fragment Length Polymorphism (T-RFLP).9. The method according to claim 1 , wherein the primer is used in association with at least one other primer which targets an 18S rDNA/ITS1 region or an ITS2/28S rDNA region of a fungus.10. The method according to claim 9 , wherein the other primer has a nucleic acid sequence of one of SEQ ID NOs: 39 to 42.12. The method according to claim 1 , wherein the primer has a nucleic acid sequence of one of SEQ ID NO: 1 to 38 claim 1 , or a complementary sequence thereof. The present application is a divisional of U.S. application Ser. No. 12/737,006, filed Dec. 1, 2010, which is a U.S. National Phase of PCT Application No. PCT/EP2009/051689, filed May 20, 2009, which claims the benefit of EP Application No. 08104225.1 and U.S. Provisional Application No. 61/059,862, ...
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