14-07-2010 дата публикации
Номер: CN0101775443A
Автор:
HAN WEI,
LI XIULI,
REN WEIKE,
SUN YINGFENG,
WANG DONG,
YAN MINGHUA,
ZHANG LI,
WEI HAN,
XIULI LI,
WEIKE REN,
YINGFENG SUN,
DONG WANG,
MINGHUA YAN,
LI ZHANG
Принадлежит:
The invention relates to a LAMP kit for detecting PRV, an establishment method and an application thereof. The kit comprises a detection system consisting of LAMP reaction solution of four primers. Detection has verified that the kit can quickly and conveniently detect the PRV with high efficiency, high specificity and high sensitivity under 60 to 65DEG C isothermal condition, does not need any complicated instrument, can well meet the requirement on the field detection to the PRV, can distinguish PRV gE gene deletion vaccine and field strains, provides a novel technical platform for the safety detection of food, can well meet the urgent need on the field detection of the PRV, is used for the field detection of import and export quarantine, food health departments, livestock farms and the like, and is easy to be popularized and applied in a large scope.
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