11-04-2023 дата публикации
Номер: CN115947794A
Принадлежит:
The invention discloses a PCV2ORF3 specific polypeptide and application thereof, and relates to the technical field of biology. The invention discloses a PCV2ORF3 specific polypeptide. The amino acid sequence of the PCV2ORF3 specific polypeptide is as shown in SEQ ID NO. 1. The invention also discloses an application of the specific polypeptide in preparation of an ELISA kit for detecting a PCV2 antibody. The ORF3 protein is selected as a target antigen, the epitope of the ORF3 protein is predicted and analyzed through a molecular biology method, the specific polypeptide of the ORF3 protein is used as a coating antigen to establish an ELISA method for detecting the PCV2 antibody, the method has good repeatability, sensitivity and specificity, PCV2 wild virus infected pigs can be identified from PCV2 subunit vaccine immunized pig herds, and the PCV2 subunit vaccine immunized pig herds can be used for detecting the PCV2 antibody in the PCV2 subunit vaccine immunized pig herds. Powerful technical ...
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