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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
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Применить Всего найдено 19. Отображено 19.
27-10-1999 дата публикации

Preparation of oral liquid for rhinitis

Номер: CN0001232679A
Автор: SONG SHIPING, SHIPING SONG
Принадлежит:

The oral liquid for curing acute, chorinic and allergic rhinitis is prepared by using 14 kinds of Chinese medicinal materials including Fructus Xanthii, Fructus mune, Flos Magnoliae, Herba Agastachis, Radix Scutellariae, etc. as well as honey and chlorpheniramine. Compared with available technology, the oral liquid has the features of reasonable recipe, easy preparation, easy taking, no toxic side effect, etc. and has high effective rate.

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Номер записи: 1
23-12-2009 дата публикации

Method and kit for testing single nucleotide polymorphism by using nucleic acid ligase

Номер: CN0101608214A
Автор: FAN CHUNHAI, WAN YING, ZHANG JIONG, SONG SHIPING, CHUNHAI FAN, YING WAN, JIONG ZHANG, SHIPING SONG
Принадлежит:

The invention discloses a method for testing the single nucleotide polymorphism (SNP) of the xth base of a nucleic acid to be tested. The invention discloses chip and a kit for testing the single nucleotide polymorphism (SNP) of the xth base of the nucleic acid to be tested. The method of the invention combines the characteristics of base complementation and ligation of an oligonucleotide and the characteristic of quickness of a multi-channel electrochemical high-pass test and can quickly and accurately test the base type of an SNP locus.

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Номер записи: 2
27-07-2011 дата публикации

Micro fluid control array protein chip and its usage method

Номер: CN0101165486B
Автор: SONG SHIPING, YAN JUAN, FAN CHUNHAI, SHIPING SONG, JUAN YAN, CHUNHAI FAN
Принадлежит:

Said protein chip comprises a micro flow control channel layer and a chip substrate; at least one of both above is made of soft substance. It features: the micro flow control layer comprises two layers respectively having a first micro flow control channel and a second micro flow control channel; the first micro flow control channel is used for assembling the capturing probes; the second micro flow control channel is used for adding the tested protein sampler and the detection probes; said first micro flow channel layer and the second micro flow control channel layer are located on the chip substrate and forms 2D micro-array pattern on the chip.

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Номер записи: 3
15-12-2010 дата публикации

Unpowered micro-flow control chip for heavy metal ion on site detection and making and using method thereof

Номер: CN0101435807B
Автор: FAN CHUNHAI, SONG SHIPING, HE SHIJIANG, CHUNHAI FAN, SHIPING SONG, SHIJIANG HE
Принадлежит:

The invention discloses a multi-channel powerless microfluidic chip for field detection of heavy metal ions and a production method and a use method thereof. The microfluidic chip comprises a substrate layer and a channel sheet layer hermetically connected with the substrate layer, wherein the channel sheet layer comprises a single or more channels, and both ends of the channels are connected with sample inlet holes and sample outlet holes respectively; the substrate layer and the channel sheet layer are both made of polydimethylsiloxane materials, and the thickness of the channel sheet layeris between 8 and 20 millimeters. A sample inlet mode of the chip does not need external energy, and can achieve the field use effect only by utilizing the ability of gas storage of polydimethylsiloxane through the vacuum-pumping pretreatment. The microfluidic chip is suitable for the real-time rapid detection of different kinds of heavy metal ions in the field when being combined with different kinds ...

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Номер записи: 4
18-02-2009 дата публикации

Quantum point biological probe and preparation method thereof, and microcurrent control protein chip based on the same

Номер: CN0101368943A
Автор: FAN CHUNHAI, WANG LIANHUI, SONG SHIPING, HE YAO, YAN JUAN, HUANG WEI, CHUNHAI FAN, LIANHUI WANG, SHIPING SONG, YAO HE, JUAN YAN, WEI HUANG
Принадлежит:

The invention discloses a quantum dot bio-probe and a preparation method thereof. The bio-probe is a quantum dot-biological composite probe formed by the quantum dot nano-microspheres and the target protein ligands which are connected through coupling reaction; and the quantum dot nano-microspheres are formed by quantum dots coated with polymers. The invention also provides a micro-fluidic protein chip which uses the quantum dot-biological composite probe as a signal probe (detection probe). Through the adoption of the quantum dot nano-microspheres, the invention has higher quantum efficiency compared with simpler quantum dots and also has more stable physicochemical properties and better compatibility with biological molecules; therefore, the invention is particularly applied to the protein chips and can greatly improve the sensitivity of the chips.

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Номер записи: 5
15-09-2010 дата публикации

Target molecule detection method based on nanometer-gold and nucleic acid structure and kit thereof

Номер: CN0101832935A
Автор: FAN CHUNHAI, SONG SHIPING, WANG LIHUA, CHUNHAI FAN, SHIPING SONG, LIHUA WANG
Принадлежит:

The invention relates to a target molecule detection method based on nanometer-gold and nucleic acid structure and a kit thereof. The target molecule detection method comprises the following steps of: (1) reacting specific DNA which can be specifically combined with the target molecules with a solution to be detected; (2) mixing the reaction solution obtained in the step (1) with a nanometer-gold solution for reaction; and (3) observing the fluorescence spectrum of the reaction solution finished in the step (2). The detection method has generality, the detected object can be an arbitrary target molecule, and the application range is wide. By utilizing the invention, the target molecules can be fast, sensitively and selectively detected.

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Номер записи: 6
21-10-2009 дата публикации

Target molecule detection method based on nano-Au and nucleic acid structure

Номер: CN0101561398A
Автор: FAN CHUNHAI, WANG LIHUA, SONG SHIPING, CHUNHAI FAN, LIHUA WANG, SHIPING SONG
Принадлежит:

The invention discloses a target molecule detection method based on nano-Au and nucleic acid structure. The detection method sequentially comprises the following steps: (1) specific DNA which can specifically react with target molecules is sufficiently hybridized with the cDNA of the DNA to form a double-chain capture probe, wherein the target molecules are proteins or ions; (2) target molecule solution is added for full reaction; (3) nano-Au solution with the mol number of 0.01-1 time of that of the specific DNA is added, and the solution is red after reaction; (4) high salting solution with the final concentration of 1-100 mM is added, and the color change of the solution is observed. The detection method has commonality, wide application range, good specificity and high sensitivity, can quickly detect any proteins or any ions with low cost, and does not need DNA mark or dear instruments.

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Номер записи: 7
23-06-2010 дата публикации

Fluorescence detection method for DNA and kit thereof

Номер: CN0101240329B
Автор: LIU XINGFEN, LI JIANG, SONG SHIPING, WANG LIHUA, FAN CHUNHAI, XINGFEN LIU, JIANG LI, SHIPING SONG, LIHUA WANG, CHUNHAI FAN
Принадлежит:

The invention discloses a DNA fluorescent detection process and its agent box. In the method, target direction DNA is captured through cross-fertilizing between DNA after the capturing detecting probe is coupled to the magnetic particle surface, another nucleotide sequence of target direction DNA is cross-fertilized with semaphore detecting probe modified by luciferin to form sandwich filler structure of magnetic particle-target direction DNA-fluorescence labeling semaphore detecting probe marked, meanwhile competitiveness detecting probe of above capture detecting probe or semaphore detecting probe is added to combine with target direction DNA, finally, water-solubility conjugate high molecule with semaphore multiply function is added to amplify and detect the signal in order to detect the target direction DNA. The invention can improve greatly the selectivity and sensitivity of gene and gene mutation detection without amplifying and increasing nucleic acid and with quick and easy operation ...

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Номер записи: 8
13-05-2009 дата публикации

Electrochemical luminescence system

Номер: CN0101430285A
Автор: SONG SHIPING, FAN CHUNHAI, SHIPING SONG, CHUNHAI FAN
Принадлежит:

The invention discloses an electrochemical luminescent system, comprising tin ion or the compound thereof which is used as strong reducing agent, an illuminophore which can form excited state and illuminate by redox reaction and an electrolyte buffer system which is used as reducer and applies the redox reaction towards the illuminophore. The invention also discloses an electrochemical luminescence method of the luminescent system. The invention has the advantage that the constructed electrochemical luminescent system has the characteristics of high sensitivity, wide pH working range, component and product without toxic hazard to human body and environment, etc.

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Номер записи: 9
23-04-2008 дата публикации

Micro fluid control array protein chip and its usage method

Номер: CN0101165486A
Автор: SONG SHIPING, FAN CHUNHAI, YAN JUAN, SHIPING SONG, CHUNHAI FAN, JUAN YAN
Принадлежит:

Said protein chip comprises a micro flow control channel layer and a chip substrate; at least one of both above is made of soft substance. It features: the micro flow control layer comprises two layers respectively having a first micro flow control channel and a second micro flow control channel; the first micro flow control channel is used for assembling the capturing probes; the second micro flow control channel is used for adding the tested protein sampler and the detection probes; said first micro flow channel layer and the second micro flow control channel layer are located on the chip substrate and forms 2D micro-array pattern on the chip.

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Номер записи: 10
18-02-2009 дата публикации

Quantum point biological probe and preparation method thereof, and microcurrent control protein chip based on the same

Номер: CN0101368944A
Автор: FAN CHUNHAI, WANG LIANHUI, SONG SHIPING, HE YAO, YAN JUAN, HUANG WEI, CHUNHAI FAN, LIANHUI WANG, SHIPING SONG, YAO HE, JUAN YAN, WEI HUANG
Принадлежит:

The invention discloses a quantum dot bio-probe and a preparation method thereof. The bio-probe is a quantum dot-biological composite probe formed by the water-soluble quantum dots and the target protein ligands which are connected through coupling reaction. The invention also provides a micro-fluidic protein chip which uses the quantum dot-biological composite probe as a signal probe (detection probe). Through the adoption of the water-soluble quantum dots, the invention has better affinity with protein and other biological molecules and can obtain a quantum dot-biological composite probe with higher performances through the highly efficient coupling between the water-soluble quantum dots and the biological molecules; in addition, as the water-soluble quantum dots have no organic reagent residue, the damage to the activity of protein and other biological molecules can be minimized, and the combination properties of the biological probe can be better exerted.

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Номер записи: 11
23-02-2005 дата публикации

Immune microarray protein chip

Номер: CN0001584592A
Автор: SONG SHIPING, LI BIN, WANG HUIQIONG, SHIPING SONG, BIN LI, HUIQIONG WANG
Принадлежит:

An immune microarray protein chip is prepared by printing antibody taken from antibod library or prepared antibody fragment on chip substrate with aldehyde group, fixing antibody or antibody fragment on activated chip substrate by plate printing. The universal signal matter is applied and only one type of signal tag is used for carrying out protin analysis when the chip is used for detection.

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Номер записи: 12
06-04-2011 дата публикации

Electrochemical biosensor and detecting method thereof

Номер: CN0102004126A
Автор: Meng Weili, Song Shiping, Jiang Zhineng, Gu Ruifeng, Zhu Xinhua, Su Yan, Fan Chunhai
Принадлежит:

The invention discloses an electrochemical biosensor and a detecting method thereof. The sensor comprises a multi-channel chip electrode, an electrochemical detecting module and a control module, wherein the multi-channel chip electrode is used as the front end of the electrochemical biosensor and converts chemical signals into electric signals; the electrochemical detecting module comprises a signal conditioning circuit, an A/D (analog/digital) converter and an MCU (micro controller unit); the electrochemical detecting module generates a voltage and inputs the voltage into the chip electrode; the chip electrode generates the corresponding current; the current is converted into the voltage through the signal conditioning circuit; the voltage passes through the A/D converter to obtain the corresponding digital amount and then is processed by the MCU; and the control module is used for taking charge of controlling the testing process, realizing the real-time display, controlling the experiment ...

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Номер записи: 13
20-05-2009 дата публикации

Unpowered micro-flow control chip for heavy metal ion on site detection and making and using method thereof

Номер: CN0101435807A
Автор: FAN CHUNHAI, SONG SHIPING, HE SHIJIANG, CHUNHAI FAN, SHIPING SONG, SHIJIANG HE
Принадлежит:

The invention discloses a multi-channel powerless microfluidic chip for field detection of heavy metal ions and a production method and a use method thereof. The microfluidic chip comprises a substrate layer and a channel sheet layer hermetically connected with the substrate layer, wherein the channel sheet layer comprises a single or more channels, and both ends of the channels are connected with sample inlet holes and sample outlet holes respectively; the substrate layer and the channel sheet layer are both made of polydimethylsiloxane materials, and the thickness of the channel sheet layer is between 8 and 20 millimeters. A sample inlet mode of the chip does not need external energy, and can achieve the field use effect only by utilizing the ability of gas storage of polydimethylsiloxane through the vacuum-pumping pretreatment. The microfluidic chip is suitable for the real-time rapid detection of different kinds of heavy metal ions in the field when being combined with different kinds ...

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Номер записи: 14
26-01-2011 дата публикации

Electrochemical luminescence system

Номер: CN0101430285B
Автор: FAN CHUNHAI, SONG SHIPING, CHUNHAI FAN, SHIPING SONG
Принадлежит:

The invention discloses an electrochemical luminescent system, comprising tin ion or the compound thereof which is used as strong reducing agent, an illuminophore which can form excited state and illuminate by redox reaction and an electrolyte buffer system which is used as reducer and applies the redox reaction towards the illuminophore. The invention also discloses an electrochemical luminescence method of the luminescent system. The invention has the advantage that the constructed electrochemical luminescent system has the characteristics of high sensitivity, wide pH working range, component and product without toxic hazard to human body and environment, etc.

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Номер записи: 15
23-07-2008 дата публикации

Highly concentrated nano-Au solution, concentrating method and applications thereof

Номер: CN0101224502A
Автор: FAN CHUNHAI, WANG LIHUA, SONG SHIPING, CHUNHAI FAN, LIHUA WANG, SHIPING SONG
Принадлежит:

The invention discloses a concentration method of a nano gold solution, comprising that a nano gold solution which is prepared by a chlorine gold acid solution is reduced by a reducing agent and further concentrated by a high-speed centrifugation. The centrifugal rotation speed of the high-speed centrifugation ranges from 8000-16000rpm and the centrifugation time ranges from 10-30 minutes. The invention also provides a high-consistency nano gold solution which is prepared by the concentration method; wherein, the nano gold grain granularity is uniformly distributed and the solution has higher salt tolerance. The high-consistency nano gold solution of the invention is applied to a DNA molecule self-assembly and the prepared Au-DNA composite matter has better acid tolerance and stability.

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Номер записи: 16
13-08-2008 дата публикации

Fluorescence detection method for DNA and kit thereof

Номер: CN0101240329A
Автор: SONG SHIPING, LIU XINGFEN, LI JIANG, WANG LIHUA, FAN CHUNHAI, SHIPING SONG, XINGFEN LIU, JIANG LI, LIHUA WANG, CHUNHAI FAN
Принадлежит:

The invention discloses a DNA fluorescent detection process and its agent box. In the method, target direction DNA is captured through cross-fertilizing between DNA after the capturing detecting probe is coupled to the magnetic particle surface, another nucleotide sequence of target direction DNA is cross-fertilized with semaphore detecting probe modified by luciferin to form sandwich filler structure of magnetic particle-target direction DNA-fluorescence labeling semaphore detecting probe marked, meanwhile competitiveness detecting probe of above capture detecting probe or semaphore detecting probe is added to combine with target direction DNA, finally, water-solubility conjugate high molecule with semaphore multiply function is added to amplify and detect the signal in order to detect the target direction DNA. The invention can improve greatly the selectivity and sensitivity of gene and gene mutation detection without amplifying and increasing nucleic acid and with quick and easy operation ...

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Номер записи: 17
20-08-2008 дата публикации

Nano-gold signal probe for DNA detection, production method and method for detecting DNA

Номер: CN0101245387A
Автор: FAN CHUNHAI, LIU XINGFEN, WANG LIHUA, SONG SHIPING, CHUNHAI FAN, XINGFEN LIU, LIHUA WANG, SHIPING SONG
Принадлежит:

The invention discloses a nano-gold signal probe for DNA detection and the preparation method thereof, as well as a DNA detection method; the nano-gold signal probe is that the surface thereof is simultaneously assembled with proteins which can generate chromogenic or fluorescence signals and a sulfhydryl-modified signal probe DNA nano-gold-particle solution. A solid phase carrier is utilized in the DNA detection, and a sandwich complex of the solid phase carrier-target DNA-nano-gold signal probe is formed by DNA hybridization. As the surface of one nano-gold particle can be connected with a plurality of proteins which can generate chromogenic or fluorescence signals, one protein which can generate chromogenic or fluorescence signals, especially an enzyme catalyst, can catalyze a plurality of substrates to have chemical reactions, so as to amplify reaction signals, and further to significantly improve detection sensitivity. Therefore, the nano-gold signal probe, the preparation method and ...

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Номер записи: 18
28-11-2007 дата публикации

DNA electrochemical sensor and preparation method thereof

Номер: CN0101078026A
Автор: FAN CHUNHAI, ZHANG JIONG, SONG SHIPING, CHUNHAI FAN, JIONG ZHANG, SHIPING SONG
Принадлежит:

The invention describes a kind of DNA electrochemistry sensor, which comprises gold pole, complementary DNA, gold nano-particles and electrochemistry indicator. It is characterized in that one segment of complementary DNA is assembled onto the gold pole as capture probe, and the other segment as detection probe is integrated with the gold nano-particles to be the carrier of electrochemistry indicator. The preparation method is disclosed. By means of 'sandwich' hybridization method, the surface of the pole has high DNA chains with negative charge and The VIII family transition metal ions with positive charge was introduced to combine with DNA as electrochemistry indicator. The DNA hybridization reaction is indicated by detecting the electrochemistry numerical change (electric current or quantity) of the pole surface. Because of the amplification effect of the gold nano-particles, the sensor can detect trace DNA with high selectivity and the gold pole modified with DNA can be used repeatedly ...

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Номер записи: 19