Method for preparing molybdenum disulfide nanosheets by ultrasonic-assisted chemical intercalation

The invention provides a method for preparing molybdenum disulfide nanosheets by ultrasonic-assisted chemical intercalation and belongs to the field of preparation of molybdenum disulfide nanosheets. The method provided by the invention comprises the following steps: performing ultrasonic reaction on molybdenum disulfide powder and n-butyl lithium under the protection of high-purity argon gas to obtain lithium-intercalated molybdenum disulfide; and adding ultra-pure water into a reactant, performing chemical stripping on a molybdenum disulfide bulk material to obtain single-layer molybdenum disulfide nanosheets, adding anhydrous ethanol for washing, and performing ultra-pure water dispersion and high-speed centrifugal separation so as to obtain a single-layer molybdenum disulfide nanosheet material which can be dispersed in water. Compared with the traditional method for preparing the molybdenum disulfide nanosheets by chemical intercalation, the method for preparing the molybdenum disulfide ...

Chirita brachytricha tissue culture and rapid propagation method

The invention discloses a chirita brachytricha tissue culture and rapid propagation method comprising the following steps: establishing an explant; performing a sprout induction stage; performing a proliferation culture stage; performing a strong seedling rooting stage; and performing seedling hardening and transplantation, wherein transplanted chirita brachytricha test-tube seedlings have the height of 3-4cm, the test-tube seedlings can grow normally under the conditions that the temperature is kept at 15-25 DEG C, the humidity is more than 70%, and 78-80% of sun-shading and ventilation can be achieved, and the transplanting survival rate of the seedlings can reach more than 93%; and the management and protection of plant diseases and insect pests can be enhanced by frequently applying thin fertilizers, and a large-scale tissue culture and rapid propagation method of the seedlings of chirita brachytricha can be established. By adopting the method disclosed by the invention, the propagation ...

Method for detecting virus by electrochemical sensor based on framework nucleic acid and CRISPR

The invention provides a method for detecting viruses by an electrochemical sensor based on framework nucleic acid and CRISPR (clustered regularly interspaced short palindromic repeats), and belongs to the technical field of virus detection. Comprising the following steps: S1, preparing framework nucleic acid with electrochemical signal molecules; s2, pretreating a gold electrode, and connecting a sulfydryl-modified hairpin chain SH-H1 to the surface of the gold electrode through an Au-S bond to obtain an electrochemical sensing interface; s3, preparing a CRISPR (clustered regularly interspaced short palindromic repeats)/Cas13a cutting system, and adding a hairpin H0 into the CRISPR/Cas13a cutting system; s4, performing mixed incubation on an electrochemical sensing interface and a product obtained in S3, adding a hairpin H2, and performing CHA reaction on the electrochemical sensing interface; and S5, adding the product obtained in S1 into the product obtained in S4, carrying out mixed ...

Near-infrared conjugated polymer as well as preparation method and application thereof

The invention provides a near-infrared conjugated polymer as well as a preparation method and application thereof. The polymer structure represents a compound. The quinoxaline thiadiazole derivative is used as a main structure, so that the polymer has the advantages of near-infrared absorption and emission, high stability, high photothermal conversion efficiency and the like. The method has great application prospects in the aspects of photovoltaic devices, fluorescence imaging, photodynamic therapy, photothermal therapy, photoacoustic imaging and the like.

Enzyme-integrated hydrogel composite material, preparation method thereof and application of enzyme-integrated hydrogel composite material in preparation of dual-mode glucose detection kit

The invention relates to the technical field of biosensing, in particular to an integrated enzyme hydrogel composite material, a preparation method thereof and application of the integrated enzyme hydrogel composite material in preparation of a dual-mode glucose detection kit. The integrated enzyme hydrogel composite material disclosed by the invention is prepared by taking hydrogel, nano-enzyme with glucose oxidase-like properties, horse radish peroxidase and fluorescent dye as raw materials. According to the invention, the fluorescent dye can be embedded in the agarose hydrogel, and the quantitative relationship between the glucose concentration and the fluorescence signal change can be obtained by utilizing active oxygen mediated fluorescence signal change generated by cascade catalysis of the integrated enzyme on a target object. In addition, active oxygen generated by cascade catalysis of the integrated enzyme on a target object can also oxidize a common colorimetric chromogenic substrate ...

Deep-permeable nano-enzyme capsule as well as preparation method and application thereof

The invention belongs to the technical field of nano biological medicines, and relates to a deep-permeable nano enzyme capsule as well as a preparation method and application thereof. The preparation method of the nano-enzyme capsule comprises the following steps: firstly, coordinating ferrous iron of FeCl24H2O with nitrogen atoms in 5, 10, 15, 20-tetra (4-pyridyl) porphyrin to obtain Fe-TPYP; then pyridyl in the Fe-TPYP reacts with bromine atoms in 1, 3-dibromopropane to obtain Fe-TPYP-C3, and finally the Fe-TPYP-C3 reacts with hyaluronic acid to obtain HA-Fe-TPYP-C3; the preparation method is simple and easy to operate; the HA-Fe-TPYP-C3 converts hydrogen peroxide into active oxygen under an acidic condition by utilizing the activity of peroxidase, so that the level of the active oxygen in a solid tumor can be increased, and the chemical kinetic treatment effect of the solid tumor is enhanced; the penetration depth of the nano-enzyme at a dense tumor part and the effect of chemical kinetics ...

Application of cyclopalladated ferrocenylimine-phosphine adduct in synthesis of asymmetric biaryl compound

The invention belongs to the technical field of organic synthesis, and in particular relates to an application of a cyclopalladated ferrocenylimine-phosphine adduct in synthesis of an asymmetric biaryl compound. In synthesis of the asymmetric biaryl compound, the cyclopalladated ferrocenylimine-phosphine adduct is utilized as a catalyst, and the catalyst has the advantages of good stability, efficient catalytic activity and wide applicability; and by utilizing the catalyst, a corresponding coupling product with high yield (up to 95%) can be obtained on the premise of smaller catalytic amount, and the synthesis process has the characteristics of mild reaction conditions, wide substrate range and strong reaction specificity.

Preparation method of polyaniline-graphene composite material

The invention discloses a preparation method of a polyaniline-graphene composite material, which comprises the following steps: 1. preparing graphite oxide by an improved Hummers process; 2. dispersing 15-25mg of the graphite oxide in a 1.0 mol/L H2SO4 solution; 3. stirring or carrying out ultrasonic treatment for 1 hour; 4. adding 0.15-0.25g of aniline monomer, and stirring for 1 hour; 5. adding 0.35-0.45g of oxidizer into an ammonium persulfate acid solution, stirring uniformly, transferring into a high-pressure reaction kettle, and reacting at 140 DEG C for 12 hours; and 6. washing, centrifugating, and drying to obtain the polyaniline-graphene composite material. The synthesis method of the nanowire and conical polyaniline-graphene composite material is simple, and has the advantages of accessible raw materials and mild conditions. When the material is applied to the supercapacitor electrode material, the experimental result indicates that the supercapacitor has the advantages of high ...

Up-conversion luminescence-based chip detection system and reagent for gynecological tumor markers CA125, CA153, SCCA, HE4

The invention relates to an up-conversion luminescence-based detection system for four gynecological tumor markers (CA125, CA153, SCCA, HE4), and the detection is performed by using a reaction cup detection system. The reaction cup detection system comprises a carrier plate, a reaction detection cup, and a fluorescence detector. The reaction detection cup is composed of a reaction detection cup and a test tube cover. A sample is dropwise added into the reaction detection cup for detection; after reaction for 5-15 min, the carrier plate is taken out and inserted into a slot of the fluorescence detector for value reading. The invention is characterized in that: the detection of four gynecological tumor markers CA125, CA153, SCCA, HE4 is carried out simultaneously by combining with an up-conversion luminescence detection method. The background value is small, and the measurement is rapid, accurate, and simple in operation. Data are transmitted to a doctor through wifi so as to obtain the detection ...

CRISPR (clustered regularly interspaced short palindromic repeats) technology-based fluorescent visual virus nucleic acid detection kit as well as preparation method and application thereof

The invention discloses a fluorescent visual virus nucleic acid detection kit based on a CRISPR (clustered regularly interspaced short palindromic repeats) technology as well as a preparation method and application of the fluorescent visual virus nucleic acid detection kit. The kit comprises a recognition module and a signal module, the recognition module is a gene editing probe constructed by utilizing a CRISPR (clustered regularly interspaced short palindromic repeats) technology and comprises mediated crRNA and Cas protein aiming at a viral nucleic acid target, and the mediated crRNA has a sequence targeting the viral nucleic acid, can be incubated with the Cas protein in advance to obtain a Cas-crRNA compound and is used for recognizing the viral nucleic acid target; and the signal module is a magnetic probe constructed by utilizing fluorescence-labeled single-stranded nucleic acid modified magnetic beads, consists of the magnetic beads and fluorescence-labeled single-stranded nucleic ...

Near-infrared luminescent compound, preparation method thereof and biosensor

The invention provides a near-infrared luminescent compound, a preparation method thereof and a biosensor, the structural general formula (I) of the near-infrared luminescent compound is shown in the specification, in the formula, L11, L12, L21 and L22 are independently selected from a single bond, a double bond, a C6-C30 arylene group, a substituted C6-C30 arylene group, a C3-C30 heteroarylene group or a substituted C3-C30 heteroarylene group; ar11, Ar12, Ar13 and Ar14 are respectively and independently selected from a C6-C30 aryl group, a substituted C6-C30 aryl group, a C3-C30 heteroaryl group or a substituted C3-C30 heteroaryl group. Compared with the prior art, the probe molecular material prepared by the invention has the advantages of high sensitivity, good selectivity, short response time, good membrane permeability, low background noise, capability of directly observing fluorescence and ultraviolet visible spectrums, capability of being applied to cell imaging and living imaging ...

Nanometer material for hunger treatment combined with copper death as well as preparation method and application of nanometer material

The invention belongs to the technical field of nano materials and anti-tumor treatment medicines, and particularly relates to a nano material for treating hunger treatment and copper death as well as a preparation method and application of the nano material. The nano material is prepared by wrapping a metal organic framework (MOF-199) outside manganese dioxide (MnO2) nano particles and modifying glucose oxidase (GOx) outside a shell layer of the MOF-199. After the nano material is internalized by cancer cells, on one hand, glucose is consumed to achieve the purpose of hunger treatment, on the other hand, MOF-199 shells are degraded by glutathione in the cancer cells, exposed MnO2 plays a role in catalase activity catalysis to generate oxygen, and meanwhile, mitochondrial function loss is caused by sharp increase of Cu (I) in the cells and oligomerization of fat acylated protein S-acetyltransferase (DLAT). Copper death is caused; the invention not only realizes cancer treatment, but also ...

Kit for simultaneously detecting double sites of coronavirus nucleic acid as well as preparation method and application of kit

The invention discloses a kit for simultaneously detecting double sites of coronavirus nucleic acid as well as a preparation method and application of the kit. The kit comprises a double-site detection probe and catalytic probes C1 and C2, the double-site detection probe comprises a nanoparticle carrier, a first site recognition unit and a second site recognition unit, wherein the first site recognition unit is composed of a DNA anchoring chain AP, a DNA connecting chain L1 and a DNA fluorescent report chain R1, and the second site recognition unit is composed of a DNA connecting chain L2 and a DNA output chain R2. The two site recognition units are complementary through L1 and L2 partial sequences to form a composite hybrid; the composite hybrid is connected with a nanoparticle carrier through AP to form a double-site detection probe; the sequences of the L1 and the L2 are respectively complementary with two sections of coronavirus nucleic acid characteristic sequences T1 and T2 to be ...

Portable African swine fever virus nucleic acid detection kit based on CRISPR (clustered regularly interspaced short palindromic repeats) technology as well as preparation method and application thereof

The invention discloses a portable African swine fever virus nucleic acid detection kit based on a CRISPR technology and a preparation method and application thereof. The kit comprises a gene editing probe, a reaction solution, ABTS and hydrogen peroxide, the gene editing probe comprises an RNA sequence crRNA complementary with an African swine fever nucleic acid characteristic sequence, a Cas12a protein and an initiation chain, the reaction liquid comprises a hairpin 1, a hairpin 2 and hemin; wherein the crRNA, the initiation chain, the hairpin 1 and the hairpin 2 respectively have sequences as shown in SEQ ID No.1-4. On the basis of CRISPR/Cas12a system accurate recognition, on-site rapid visual detection of the African swine fever virus can be achieved through hydrogen peroxide catalytic color development, and compared with a traditional PCR nucleic acid detection method, the method is easy to operate, the detection result is easy to read, the method does not depend on professional instruments ...

Cantilever beam type VOC gas sensor based on polyethylene film and manufacturing method of cantilever beam type VOC gas sensor

The invention provides a cantilever beam type VOC gas sensor based on a polyethylene film and a manufacturing method thereof, the method comprises the following steps: step 1, manufacturing a cantilever beam, the cantilever beam comprising a support layer, a bonding layer and a functional layer; step 2, manufacturing a sensor chamber; 3, adjusting a laser and a fixed-focus camera, and establishing a detection light path; step 4, connecting a gas circuit, pumping out the gas to be detected from the sample bag by using a micro gas pump, and sending the gas to be detected into a sensor chamber; 5, a sensor detection circuit is built for detection, a humidity sensor, a power source, a data transmission line, a gas sampling bag, a gas pump, a computer and the like are connected to the detection circuit, and a hygrometer, a flow meter and a camera are uniformly read in real time by a computer end self-programmed program. The cantilever beam type VOC gas sensor based on the polyethylene film, ...

cryIAc gene having high lepidopteran pest resistance and application thereof in sugarcane

The invention belongs to the fields of gene engineering and biological control, and particularly relates to an artificially synthesized Bt gene and application of a transgenic plant thereof in pest resistance. The invention provides a DNA molecule which can code the cryIAc gene having high lepidopteran pest resistance to obtain mcryIAc, and the DNA molecule has a nucleotide sequence shown in SEQ ID NO1. The invention also provides a corresponding protein and plant expression vector. In the invention, the cryIAc gene is completely modified, a plant preferred codon is used, and elements interfering with the expression of the gene in the plant are removed from the original sequence, so that a transgenic sugarcane strain having high expression quantity is obtained; and greenhouse and field experiments prove that the transgenic sugarcane strain has natural resistance to lepidopteran pests. The breeding of the anti-borer sugarcane variety is beneficial to reducing the threat of borers to sugarcane ...

Vehicle fuel gas alarm, alarm method and vehicle comprising vehicle fuel gas alarm

The invention discloses a vehicle fuel gas alarm and an alarm method of the vehicle fuel gas alarm. The vehicle fuel gas alarm comprises a detector, a power supply control circuit and a processor. The detector is arranged in a vehicle, detects the concentration of hazardous gas in the vehicle through a sensor, and outputs detection data. The power supply control circuit is connected with a vehicle battery and a vehicle key switch and outputs electric signals according to the state of the voltage of the vehicle battery and the state of the vehicle key switch. The processor is connected to the detector and an alarm control circuit, according to the state signals, output by the power supply control circuit, of the vehicle key switch, the processor controls the alarm to be automatically switched on after the vehicle is started and controls the alarm to be automatically switched off within a preset time period after the vehicle is parked, and the processor conducts analysis processing according ...

Hydrangea-shaped gold nanoparticle and preparation method thereof

The invention discloses a hydrangea-shaped gold nanoparticle and a preparation method of the hydrangea-shaped gold nanoparticle. The method includes the steps that a chloroauric acid solution is added into a surface active agent hexadecyl trimethyl ammonium chloride solution, and magnetic stirring is performed; then an ascorbic acid solution is added, magnetic stirring is performed, growth temperature is adjusted and controlled, standing growth is performed, and the hydrangea-shaped gold nanoparticle is obtained through purification after an obtained solution is centrifuged and washed. The average diameter is 490 nm-812 nm, and the thickness of pedals is 20 nm-44 nm. The prepared gold nanoparticle is of the hydrangea-shaped structure and is high in yield and good in monodispersity and stability, the appearance and the optical property of the particle are controllable and adjustable, and the gold nanoparticle further has the advantages of being simple in synthetic method, mild in synthetic ...

Method and kit for detecting food-borne bacteria based on RAA and LbCas12/crRNA

The invention discloses a method for detecting food-borne bacteria based on RAA and LbCas12/crRNA and a kit thereof, and the method comprises the following steps: adding a buffer solution, a forward primer, a reverse primer, a detection sample containing bacterial nucleic acid and magnesium acetate into an RAA freeze-dried powder reaction tube, and carrying out a constant temperature reaction for 15 min; after the reaction is completed, adding the RAA product into the LbCas12a/crRNA reaction system, and carrying out constant-temperature reaction for 10 minutes; and immersing the lateral flow chromatography test paper into the reaction liquid for 3-5 minutes, taking a picture of the test paper strip by using a smart phone camera, and automatically reading a detection result by using a mobile phone APP. Compared with a traditional detection method, the method has the advantages that the detection time is greatly shortened, the detection precision is improved, and the food-borne bacterium ...

Quantum point biological probe and preparation method thereof, and microcurrent control protein chip based on the same

The invention discloses a quantum dot bio-probe and a preparation method thereof. The bio-probe is a quantum dot-biological composite probe formed by the quantum dot nano-microspheres and the target protein ligands which are connected through coupling reaction; and the quantum dot nano-microspheres are formed by quantum dots coated with polymers. The invention also provides a micro-fluidic protein chip which uses the quantum dot-biological composite probe as a signal probe (detection probe). Through the adoption of the quantum dot nano-microspheres, the invention has higher quantum efficiency compared with simpler quantum dots and also has more stable physicochemical properties and better compatibility with biological molecules; therefore, the invention is particularly applied to the protein chips and can greatly improve the sensitivity of the chips.

Preparation method of flower-like gold nanoparticles and application of flower-like gold nanoparticles serving as SERS (surface enhanced Raman scattering) enhanced substrates

The invention relates to a preparation method of flower-like gold nanoparticles and an application of the flower-like gold nanoparticles serving as SERS (surface enhanced Raman scattering) enhanced substrates. The preparation method comprises steps as follows: (1), an aqueous solution containing chloroauric acid and a surfactant is prepared, the concentration of chloroauric acid is 1-20 mM, and the concentration of the surfactant is 10-300 mM; (2), silver nitrate solution with the concentration of 10 mM is added and the mixture reacts for 0.5-5 min; (3), an ascorbic acid solution with the concentration of 0.3 M is added and the mixture reacts for 0.5-5 min; (4), after a reaction system is left to stand at the temperature of 4 DEG C and grows for 5-180 min, centrifugation and washing are performed, the obtained gold nanoparticles with flower-like structures have controllable shapes and uniform sizes and can be used as the SERS enhanced substrates; besides, the process consumes short time ...

Controllable assembly method of plasmon polariton baseball 'corn' structure

The invention provides a controllable assembly method of a plasmon polariton baseball 'corn' structure. Nanorods made of noble metals such as Au, Ag and Cu and current synthetic small molecules are modified with strepavidin through covalent binding with S, the surfaces of nanoparticles made of noble metals such as Au, Ag and Cu are modified with biotin through a peptide chain, and finally, through a specific reaction between streptavidin and biotin, the nanorods and the nanoparticles are orientedly assembled by adjusting reaction amount, reaction time and reaction conditions. Characterization results of a transmission electron microscopy show that the nanorods and the nanoparticles, which are made of noble metals such as Au, Ag and Cu, are assembled into 'core' structures, the shape is uniform, and the dispersibility is high; an ultraviolet-visible absorption spectrum shows that the plasmon peak of the surface of the plasmon polariton baseball 'corn' structure is positioned in a near infrared ...

Method of preparing CdTe/CdS/ZnS core-shell-core structure quantum points

The present invention belongs to the field of nanometer material and biological analysis and detection technology, and is especially preparation process of water soluble CdTe/CdS/ZnS quantum dot in core-shell-shell structure. The preparation process includes mixing zinc salt or zinc oxide with water soluble sulfhydryl compound in water phase, injecting prepared solution of CdTe/CdS in core-shell structure to obtain CdTe/CdS/ZnS precursor solution, setting the precursor solution in a glass tube and performing microwave radiation reaction in a microwave reactor to obtain CdTe/CdS/ZnS quantum dot in core-shell-shell structure. The process is completed in water phase, and is safe and simple. The CdTe/CdS/ZnS quantum dot in core-shell-shell structure has high fluorescence quantum efficiency, high water solubility, excellent stability and high biocompatibility, and may be applied widely in fluorescent marker for biological analysis and detection.

Method for rapidly identifying resistance of transgenic Bt sugarcane to borer

The invention relates to a rapid identification method of transgenic Bt sugarcane resistance to Stem Borers by artificial inoculating. The method includes planting sugarcane in buckets in greenhouse, choosing insect source, artificial inoculating and evaluation of resistance. The experimental insect source of the same batch transgenic Bt sugarcane strain is the newly hatched larvae of the same striped sugarcane borer, or equally divides the newly hatched larvae of 2-3 female moth born to each sugarcane to be analyzed. The result can be investigated after 5 days of artificial inoculating to the sugarcanes planted in buckets in greenhouse and the pest resistance of the sugarcane strain is evaluated comprehensively according to the larval survival rate and sugarcane damage level. The most notable characteristics of the invention are: 1. the consistency and unity of inoculating time are guaranteed to the utmost extent to ensure the accuracy of identification; 2. The periodicity of pest resistance ...

Engineering fusion membrane bubble as well as preparation method and application thereof

The invention provides a preparation method of engineered fusion membrane bubbles, the fusion membrane bubbles obtained according to the preparation method and application of the fusion membrane bubbles in targeted ultrasound contrast of atherosclerotic plaques. The method comprises the following steps: treating cells by using bafilomycin A1 (BAF), and extracting cell membranes by using a cell membrane protein extraction kit; preparing lipidosome by a rotary evaporation method; preparing a fusion membrane from the extracted cell membrane and lipidosome by using a liquid nitrogen repeated freezing and thawing method; and preparing fusion membrane bubbles from the prepared fusion membrane by using an ultrasonic-assisted method. According to the method, BAF is added in the process of culturing HepG2 cells in vitro to up-regulate expression of APOA1, then cell membranes of the HepG2 cells are extracted to be fused with artificially synthesized lipidosome, and finally fused membrane bubbles ...

Preparation method of water soluble CdTe/CdS nuclear/shell type quantum point

Composite nano material capable of activating calcium overload and tumor cell pyroptosis as well as preparation method and application of composite nano material

The invention relates to the field of preparation of biomedical nano-materials, in particular to a novel composite nano-material capable of activating calcium overload and tumor cell pyroptosis and a preparation method and application of the novel composite nano-material. The composite nano material comprises calcium peroxide, a zeolite imidazate skeleton structure material, curcumin and hyaluronic acid, the calcium peroxide is embedded in the zeolite imidazate skeleton structure material to form a core-shell structure, the curcumin is loaded in a shell layer of the core-shell structure, and the hyaluronic acid is connected to the surfaces of particles. The composite material provided by the invention releases a large amount of calcium ions by responding to tumor cell lysosome acid environment degradation, so that tumor cell calcium overload is caused, and tumor cell pyroptosis is activated. The preparation method disclosed by the invention is simple and reliable, and the prepared composite ...

Ferrocenyl cycloimine palladium-phosphine adduct and preparation and application therefore

The invention belongs to synthesis of a catalyst and application therefore in organic synthesis, in particular to a ferrocenyl cycloimine palladium-phosphine adduct and preparation and application thereof. In the synthesis of arylboronic acid ester, the ferrocenyl cycloimine palladium-phosphine adduct is used as a catalyst which has favorable stability, high-efficiency catalytic activity and wide applicability and can be used for synthesizing a corresponding coupling product with high yield (as high as 93 percent) on the premise of lower catalytic amount. The invention has the advantages of mild reaction condition, wide substrate range and strong reaction specificity.

Microwave-assisted synthesis method of macromolecular polydentate ligand CdTe quantum dots

The invention discloses a microwave-assisted synthesis method of macromolecular polydentate ligand CdTe quantum dots. The method comprises the steps of synthesizing sulfydryl modified polyacrylic acid molecule, and preparing the CdTe quantum dots through microwave-assisted heating in water phase by taking the macromolecule as a ligand. In the method, dicyclohexylcarbodiimide is used as a condensation agent, sulfydryl amine is connected on the side chain of polyacrylic acid in N, N'-dimethyl formamide; sulfhydrylated polyacrylic acid and a cadmium salt or cadmium oxide are mixed in water, thus, a cadmium source solution of macromolecular polydentate ligand is obtained; potassium hydrogen telluride or sodium hydrogen telluride solution is injected into the cadmium source so as to prepare a CdTe precursor solution; the CdTe quantum dots with various emission wavelengths are prepared by means of microwave-assisted heating. The CdTe quantum dots prepared by the invention have uniform grain size ...

Distribution lines foreign matter duster

The utility model discloses a distribution lines foreign matter duster, get in touch including the wire, rotatory arc blade, the foreign matter separation blade, urceolus and inner tube, be provided with on the inner tube and insulating bar matched with connecting device, the outer peripheral face of inner tube is provided with the internal tooth, outer barrel casing is outside the inner tube, the inner wall of urceolus be provided with with the inner tube outer peripheral face on the internal tooth external tooth that cooperatees and use, set up one logical groove that a cross section along the urceolus extends on the urceolus, an arc blade sets up and stretches out on the inner tube and in the logical groove by the urceolus, the arc blade is followed the inner tube and is made swing back and forth in the logical groove of urceolus, the inner tube links to each other and drives the motion by insulating bar with insulating bar, leading to the urceolus wall of a tip in groove, the urceolus ...

Nucleus-shell-shell structured quantum dot with high fluorescence quantum efficiency and method of producing the same

The invention belongs to the technical field of nano material and biological analysis detection, and particularly discloses a method for preparing a high fluorescence quantum efficiency water soluble CdTe/CdS/Zns core-shell-shell structure quantum point by microwave. In water phase, zinc salt or zinc oxide is mixed with a water soluble sulfhydryl compound, and injected with pre-prepared CdTe/CdS core-shell structure quantum point solution to obtain CdTe/CdS/Zns precursor solution, and the precursor solution is placed in a glass pipe specially used for microwave radiation and subjected to microwave radiation reaction in a microwave reactor to obtain the CdTe/CdS/Zns core-shell-shell structure quantum point. The method is completely performed in the water phase, and is safe and convenient to operation. The obtain product has the advantages of high fluorescence quantum efficiency, good water solubility and excellent stability and biocompatibility, and can be widely applied to fluorescence ...

CRISPR/Cas12a-based detection method for detecting cardiac troponin I

The invention discloses a method for detecting cardiac troponin I (cTnI) on the basis of CRISPR (clustered regularly interspaced short palindromic repeats)/Cas12a (clustered regularly interspaced short palindromic repeats). The method comprises the following steps: hybridizing an aptamer Tro4 of cTnI with an activator Tro4'partially complementary to the aptamer Tro4, and locking the activation activity of the Tro4 '; when the target cTnI exists in a sample to be detected, the aptamer is specifically combined with the cTnI, an activator Tro4'is released, the released activator Tro4 'is combined with Cas12a/crRNA to form a compound, the trans-cleavage activity of Cas12a protein is activated, probe DNA fixed on the surface of an SPCE-Au sensor is cut, so that an electrochemical signal is reduced, and the cTnI is proved to be contained in an object to be detected. The invention aims to realize simple and rapid detection of cTnI by utilizing the specific recognition capability and efficient ...

Method for rapidly identifying resistance of transgenic Bt sugarcane to borer

The invention relates to a rapid identification method of transgenic Bt sugarcane resistance to Stem Borers by artificial inoculating. The method includes planting sugarcane in buckets in greenhouse, choosing insect source, artificial inoculating and evaluation of resistance. The experimental insect source of the same batch transgenic Bt sugarcane strain is the newly hatched larvae of the same striped sugarcane borer, or equally divides the newly hatched larvae of 2-3 female moth born to each sugarcane to be analyzed. The result can be investigated after 5 days of artificial inoculating to the sugarcanes planted in buckets in greenhouse and the pest resistance of the sugarcane strain is evaluated comprehensively according to the larval survival rate and sugarcane damage level. The most notable characteristics of the invention are: 1. the consistency and unity of inoculating time are guaranteed to the utmost extent to ensure the accuracy of identification; 2. The periodicity of pest resistance ...

Preparation and applications of graphene quantum dots with white fluorescence emission properties

The invention discloses a preparation method and applications of graphene quantum dots with white fluorescence emission properties. The preparation method comprises the following steps: a, putting nano graphite powder into a mixed acid of sulphuric acid and nitric acid, and after the mixed acid is subjected to ultrasonic processing, under the irradiation of microwaves, heating for reacting; b, filtering a reaction product in the step a by using a microporous membrane firstly, collecting filtrate, neutralizing the filtrate until the filtrate is neutral, dialyzing the filtrate for several times by using a dialysis bag, and collecting liquid in the bag, so that a graphene quantum dot is obtained; c, after the pH value of the graphene quantum dots is adjusted to 13, under the irradiation of microwaves, heating for reacting, dialyzing the obtained reaction product for several times by using a dialysis bag, and collecting liquid in the bag, so that the graphene quantum dots with white fluorescence ...

Preparation method of nano frame

The invention provides a preparation method of a nano-frame, which mainly comprises the following steps of: centrifugally washing Ag nano-particles and then re-dispersing the Ag nano-particles in ultrapure water to obtain Ag nano-particle template suspension; mixing a cationic surface active agent, a triethanolamine aqueous solution and the Ag nano-particle template suspension, and uniformly stirring; adding a sodium thiosulfate aqueous solution while stirring, and stirring at a preset temperature; adding a sodium gold thiosulfate aqueous solution while stirring, and stirring to react at a preset temperature; rapidly centrifugally separating the reaction product from the reaction system, washing and purifying with ultrapure water for multiple times, and dispersing in ultrapure water for storage; and preparing a sample, and carrying out single-particle surface enhanced Raman performance test. Compared with the prior art, the preparation process is simple, and the product is good in morphology ...

Star-shaped polymer with ion iridium complex as core, its synthesis and use

Production of star-shaped polymer with ionic iridium complex as nucleus and ATRP is simple and cheap. It can be used for organic polymer phosphorescent electroluminescent material, chemical biological sensor inspection, fluorescent micro-sphere material, and its has controllable molecular weight and structure.

Non-labeled ionic conjugated polyelectrolyte, synthetic method thereof and application to biological detection

The invention discloses design, detection application of non-labeled ionic conjugated polyelectrolyte. The non-labeled ionic conjugated polyelectrolyte has a structure shown as the general formula (I) shown in the description, and comprises a 9-substituted fluorenes unit and an acceptor unit Ar with electron-withdrawing characteristic, Ar is one substituent groups of benzo[d]thiadiazol, benzo[d]thiazol, thiazole, benzo[d]diazol, boron-dipyrromethene unit, iridium complex containing unit, metalloporphyrin containing unit, perylene bisimide, naphthalimide and the like, the ratio of fluorenes group to Ar (x:y) is 17:3-18:2, the side group comprises m+1 CH2 units, wherein Ion is one water-soluble groups such as quaternary ammonium group, carboxyl or sulfo group. The non-labeled ionic conjugated polyelectrolyte has extremely high solubility and stability, is applicable to multiple non-labeled biological detection, and is a brand-new sensing detection platform.

Automobile disc brake braking judder and squeal test bed

The invention discloses an automobile disc brake braking judder and squeal test bed which aims at solving the problem that at present, automobile braking judder and squeal cannot be tested. The automobile disc brake braking judder and squeal test bed comprises a frequency converter, a variable frequency motor, an equivalent mass regulating part, a braking pressure regulating part and a signal collecting and processing part. The variable frequency motor is installed at the left end of a left test bed body, the frequency converter is installed at the lower end of the left test bed body, and the variable frequency motor and the frequency converter are connected through an electric wire. The equivalent mass regulating part is installed at the part, on the right side of the variable frequency motor, on the left test bed body, the variable frequency motor is connected with the left end of a main shaft in the equivalent mass regulating part through a second elastic pin coupling, the braking pressure ...

Quantum point biological probe and preparation method thereof, and microcurrent control protein chip based on the same

The invention discloses a quantum dot bio-probe and a preparation method thereof. The bio-probe is a quantum dot-biological composite probe formed by the water-soluble quantum dots and the target protein ligands which are connected through coupling reaction. The invention also provides a micro-fluidic protein chip which uses the quantum dot-biological composite probe as a signal probe (detection probe). Through the adoption of the water-soluble quantum dots, the invention has better affinity with protein and other biological molecules and can obtain a quantum dot-biological composite probe with higher performances through the highly efficient coupling between the water-soluble quantum dots and the biological molecules; in addition, as the water-soluble quantum dots have no organic reagent residue, the damage to the activity of protein and other biological molecules can be minimized, and the combination properties of the biological probe can be better exerted.

Ferrocenyl cycloimine palladium-phosphine adduct and preparation and application therefore

The invention belongs to synthesis of a catalyst and application therefore in organic synthesis, in particular to a ferrocenyl cycloimine palladium-phosphine adduct and preparation and application thereof. In the synthesis of arylboronic acid ester, the ferrocenyl cycloimine palladium-phosphine adduct is used as a catalyst which has favorable stability, high-efficiency catalytic activity and wideapplicability and can be used for synthesizing a corresponding coupling product with high yield (as high as 93 percent) on the premise of lower catalytic amount. The invention has the advantages of mild reaction condition, wide substrate range and strong reaction specificity.

Method for regulating and controlling load capacity and shell thickness of magnetic particle in Fe3O4-core and SiO2-shell nanostructure

The invention discloses a method for regulating and controlling the load capacity and shell thickness of a magnetic particle in a Fe3O4-core and SiO2-shell nanostructure. The method comprises the following steps: with superparamagnetic Fe3O4 nano-particles prepared by virtue of a hydrothermal codeposition method as cores, purifying the superparamagnetic Fe3O4 nano-particles, dispersing the superparamagnetic Fe3O4 nano-particles into an alkali ethanol water solution, adding tetraethyl orthosilicate (TEOS) into the solution, regulating and controlling the number of the Fe3O4 nano-particles coated in magnetic nano-particles in a core-shell structure by controlling the adding amount of the TEOS, so as to prepare the core-shell nano-particles with adjustable saturation magnetization strength; besides, adding the TEOS into colloid of the core-shell nano-particles again, and controlling the adding amount of the TEOS, so as to further realize the adjustable growth of the shell thickness.

Engineering frozen liver cancer cell carrier as well as preparation method and application thereof

The invention provides a preparation method of an engineered frozen liver cancer cell carrier, the cell carrier obtained according to the preparation method and application of the cell carrier in the process of relieving atherosclerosis. The method comprises the following steps: stimulating hepatoma carcinoma cells cultured in vitro to express apolipoprotein ApoA1 by using bafluromycin A1; performing liquid nitrogen freezing on the treated live liver cancer cells to obtain frozen liver cancer cells; and incubating the frozen liver cancer cells and atorvastatin (AT) to obtain the frozen liver cancer cell carrier. The carrier completely loads a drug atorvastatin (AT). The frozen liver cancer cell carrier can effectively inhibit autophagy of liver cancer cells after being treated by the bajulomycin A1, so that expression of apolipoprotein (ApoA1) is promoted, and the ApoA1 and phospholipid can form beta-HDL to uptake cholesterol and transfer the cholesterol to the liver for metabolism; meanwhile ...

GO and magnetic composite material, its preparation method, and its application in water treatment

The invention discloses a preparation method of a GO (graphene oxide) and magnetic composite material, and its application in water treatment. The GO and magnetic composite material is obtained through a step of mixing the GO prepared by using expanded graphite with magnetic nano-particles, and a step of connecting by a covalent bond. The principle of the preparation method is the reaction of the carboxyl group of the GO with the terminal amino group of the coating layer of the magnetic particles. The magnetic particles are adsorbed on the surface of the GO to form the composite material. In the water purifying application, the composite material can effectively adsorb pollutants comprising organic dyes, arsenic, polybrominated aromatic hydrocarbons and the like.

Method for preparing CdTe quantum dots in aqueous phase and at low temperature

The invention belongs to the field of preparation of nano materials and relates to a method for preparing CdTe quantum dots in an aqueous phase and at a low temperature. The method comprises the following steps of: preparing solution from dithioamino formate serving as a stabilizing agent, water serving as a solvent, cadmium salt and a ligand according to a certain ratio; injecting sodium hydrogen telluride or potassium hydrogen telluride aqueous solution; and reacting in water bath at the low temperature for a certain time to obtain the water dispersible CdTe quantum dots. The preparation method has the advantages of low reaction temperature, readily available raw materials, convenience of preparation, easiness in operation, low requirement on reaction equipment, low price, energy conservation, environmental friendliness, preparation on a large scale and suitability for industrialization because the CdTe quantum dots are prepared in the aqueous phase completely. The prepared CdTe quantum ...

Expression of ckmb in Pichia pastoris and preparation

The invention discloses expression of ckmb in Pichia pastoris and an efficient preparation method. The method is characterized by carrying out construction, fermentation and purification of a carrier and engineering bacteria having an m gene and a b gene undergoing tandem expression in a same carrier in a Pichia pastoris host cell so as to prepare recombinant CKmb. The ckmb prepared by the method can be used for in vitro diagnostic reagents.

Nucleic acid sensor and preparation method thereof and multi-element detection method based on sensor

The invention discloses a nucleic acid sensor and a preparation method thereof and a multi-element detection method based on the sensor. The invention discloses the nucleic acid sensor based on magnetic nanoparticles and quantum dots. A sandwich type fluorescent detection system is constructed from a DNA probe of the magnetic nanoparticles Fe3O4@SiO2@AuNPs, target nucleic acid molecules and a DNA probe based on the quantum dots; the magnetic nanoparticles are separated from the detection system by using an externally added magnetic field, and fluorescent residues of the quantum dots in a supernatant are detected; a concentration-response curve is drawn according to the concentration of the added target nucleic acid molecules and the variation amount of the fluorescence intensity of the detection system, multiple target nucleic acid molecules are simultaneously detected, and the detection lower limit reaches a pico mole level (pM). With utilization of the quantum dot characteristics of broad ...

Single gold nanoparticle surface plasmon resonance probe and preparation method thereof

The invention discloses a single gold nanoparticle surface plasmon resonance probe and a preparation method thereof. The preparation method comprises the following steps: firstly reducing chloroauric acid by sodium citrate to obtain gold seed, diluting the obtained gold seed solution, then sequentially adding hydroxylamine hydrochloride and a chloroauric acid solution for growing spherical gold nanoparticles; secondly, uniformly fixing the spherical gold nanoparticles on the surface of a cleaned indium tin oxide (ITO) glass base, so as to prepare a gold nanoparticle surface plasmon resonance (SPR) chip; finally, fixing a hairpin-shaped single-stranded deoxyribonucleic acid (DNA) probe molecule with a specific sequence on the gold nanoparticle surface on the prepared SPR chip by virtue of covalent bonds of gold and sulphur so as to modify the SPR chip to obtain the single gold nanoparticle surface plasmon resonance probe. The diameter of the spherical gold nanoparticle is 40-90nm, and the ...

Preparation method of titanium dioxide and grapheme composite nanomaterial

The invention provides a preparation method of a titanium dioxide and grapheme composite nanomaterial. The method comprises the following steps: taking and mixing a graphene oxide aqueous solution and tert-butylamine so as to obtain mixed liquid, mixing the obtained mixed liquid with a titanium precursor solution, carrying out magnetic stirring on the obtained mixture under the condition that the obtained mixture is heated to 120-200 DEG C by way of microwave-assisted heating, carrying out a reaction on the obtained product so as to obtain an organic phase solution, and separating the titanium dioxide and grapheme composite nanomaterial from the organic phase solution, wherein the titanium precursor solution is composed of titanium propoxide, an organic coating agent and toluene. The method is used for preparing high-quality titanium dioxide and grapheme composite nanomaterial. The high quality means that nanoparticles are good in crystallinity, narrow in particle size distribution, and ...

Automobile column A with variable cross section and automobile

An automobile A column with a variable cross section comprises a base assembly, an outer decorative plate arranged on the outer side of the base assembly and a jacking assembly. The basic assembly is used for being fixedly connected between a car roof and a front cabin of a car, and the outer decoration plate comprises an outer decoration plate support and a telescopic deformation film arranged on the outer decoration plate support. When the jacking assembly extends outwards, the telescopic deformation film is opened outwards to be far away from the outer decoration plate support, and when the jacking assembly retracts inwards, the telescopic deformation film is close to the outer decoration plate support. The automobile A column with the variable cross section can effectively balance aerodynamic properties, wind noise properties and water management properties.

Microwave preparation method of water soluble ZnSe quantum dots

The invention discloses a microwave preparation method of water soluble ZnSe quantum dots, which comprises the following steps: (1) mixing solution of sodium hydrogen selenide with solution serving as a zinc source to obtain solution of ZnSe precursor; and (2) heating the solution of the ZnSe precursor by microwaves to obtain water soluble ZnSe quantum dots, wherein the microwave heating conditions include a microwave power of 50 to 300W, a heating time period of 1 to 30 minutes and a heating temperature of 70 to 120 DEG C and the solution serving as the zinc source may be solution of a zinc salt, zinc oxide or zinc hydroxide. The obtained high fluorescent quantum product has high yield, stability and water solubility and can be widely used for making fluorescent labels for biological detection and analysis.

General-type car body modal test supporting device

The invention discloses a general-type car body modal test supporting device which solves problems that the supporting position cannot be adjusted conveniently and automatically, the height cannot be adjusted when a car body is placed on a supporting device, and the stability of the car body cannot be guaranteed in the test process when the height of the car body is high. The general-type car body modal test supporting device comprises a lateral movement device, a longitudinal movement device, adjustable supporting devices and pedestals. The adjustable supporting devices comprise a No.1 adjustable supporting device, a No.2 adjustable supporting device, a No.3 adjustable supporting device and a No.4 adjustable supporting device. The longitudinal movement device is mounted on the pedestals. The No.1 adjustable supporting device and the No.2 adjustable supporting device are mounted on No.1 lateral guide rails (2-1) in the longitudinal movement device. The No.3 adjustable supporting device ...

Pickup truck vibration energy recovery unit

The utility model relates to an automotive filed, the pickup truck is applied to to specific saying so one kind, and the vibration energy that will vibrate the in -process turns into the pickup truck vibration energy recovery unit of electric energy. The device includes leaf spring, big lug, connecting rod and energy conversion mechanism, leaf spring one end is fixed on the frame, and the other end is fixed on big lug, the upper end of big lug is fixed on the frame, and the lower extreme rotates with the one end of connecting rod to be connected, the other end of connecting rod is articulated with the slider of fixing in energy conversion mechanism, energy conversion mechanism is fixed on the frame. The utility model relates to a through the improvement to the leaf spring lug to utilize energy conversion mechanism with vehicle vibration energy recuperation, be the electric energy with mechanical energy conversion, reach high -efficient purpose of retrieving the little amplitude vibration ...

Method and device for realizing Hadoop compatibility

The invention discloses a method and a device for realizing Hadoop compatibility and belongs to the field of communications. The method comprises the steps of receiving a request accessing a file on a second Hadoop system employing a second protocol, determining the version information of a main node of the file, running a compatible access interface to invoke a second method corresponding to the request in the second Hadoop system and converting an object in the second method into an object in a first method corresponding to the request in the first Hadoop system, converting the name of a packet of the second method into a packet name capable of being recognized by the first Hadoop system from a packet name capable of being recognized by the second Hadoop system, and then sending the packet name to the main node, wherein the main node belongs to a first Hadoop system employing a first protocol. The device comprises a receiving module, a determination module, a first processing module, ...