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Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

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Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

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Применить Всего найдено 9. Отображено 9.
11-02-2009 дата публикации

Method for removing Leifsonia xyli subsp.xyli and quickly breeding health cane seedling

Номер: CN0101361459A
Автор: SHEN WANKUAN, CHEN ZHONGHUA, DENG HAIHUA, LIU RUI, HE HUIYI, YANG ZHANDUAN, WANKUAN SHEN, ZHONGHUA CHEN, HAIHUA DENG, RUI LIU, HUIYI HE, ZHANDUAN YANG
Принадлежит:

The invention relates to the propagation production of cane seedlings, and provides a method for removing cane perennial root dwarfing germs and rapidly propagating healthy cane seedlings, which comprises the following steps: little amount of original seeds are treated by hot water, stem tips which are detected to be feminine by the regenerated plantlet by carrying out speckle enzyme marker immune test (DB-EIA) is taken as an explant, healthy seedlings are produced by induction culture, secondary propagation culture, root-promoting culture, temporary planting and second-stage or third-stage nursery propagation-extension; furthermore, the disinfection of seed-cutting tools (cane knives) is paid attention to in the nursery propagation-extension, before the seedlings are used as seeds for large field production after coming out from nursery, cane stem juice is extracted by sampling, and RSD virus is detected by using the DB-EIA, thus guaranteeing the providing of qualified seedlings. The method ...

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Номер записи: 1
28-01-2015 дата публикации

Infecting method for authenticating pathogenicity of sugarcane leaf fungal disease strains

Номер: CN104313111A
Автор: CHEN JIANWEN, LIU RUI, YANG ZHANDUAN, DENG HAIHUA, QI YONGWEN, XU YUANXIAN
Принадлежит:

The invention relates to the technical field of sugarcane disease inspection, and provides an infecting method for authenticating the pathogenicity of sugarcane leaf fungal disease strains. The method comprises the steps of preparation of strains, collection and pretreatment of leaves, preparation of infected leaves, infection, culture and pathogenicity evaluation of strains. The method is characterized in that the preparation step of the infected leaves comprises the substeps: shearing the leaves into long segments of 4-8cm, wrapping two ends of the leaves with tissues, putting the leaves into a sterilized culture dish, and dripping sterilized clean water onto the tissues at the two ends of the leaves; and the infection step comprises the substeps: pricking two sides of the midribs of the leaves in the culture dish by using a pricking device, clipping hyphae to the pricked positions of the leaves by using sterilized tweezers to serve as a test culture dish. The method has the following ...

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Номер записи: 2
02-11-2011 дата публикации

Method for rapidly detecting Leifsonia xyli subsp. Xyli based on nested PCR (polymerase chain reaction)

Номер: CN0102230018A
Автор: WANKUAN SHEN, RUI LIU, ZHANDUAN YANG, HAIHUA DENG, JIANWEN CHEN, ZHONGHUA CHEN, SHEN WANKUAN, LIU RUI, YANG ZHANDUAN, DENG HAIHUA, CHEN JIANWEN, CHEN ZHONGHUA
Принадлежит:

The invention discloses a method for rapidly detecting Leifsonia xyli subsp. Xyli based on nested PCR (polymerase chain reaction). Whether Leifsonia xyli subsp. Xyli exits is rapidly and accurately determined by extracting DNA of a sample, designing primers and performing the nested PCR. The detection sensitivity of the method provided by the invention reaches 100 fg genomic DNA of the Leifsonia strain and is increased by 100 to 1000 times as compared with the conventional PCR by using the same primers. The method provided by the invention is suitable for the quarantine inspection of sugarcane introduced varieties, the production of virus-free (bacterium-free) seedlings, the general survey of sugarcane ratoon stunting disease in sugarcane planting areas and so on.

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Номер записи: 3
18-08-2010 дата публикации

Method for rapidly identifying resistance of transgenic Bt sugarcane to borer

Номер: CN0101113977B
Автор: YANG ZHANDUAN, ZHANG QIONG, DENG HAIHUA, LI QIWEI, WANG LIANHUI, LAO FANGYE, LI JIAOLIANG, CHEN ZHONGHUA, CHEN HAINING, WENG LIXING, XU JINLING, SHEN WANKUAN, ZHANDUAN YANG, QIONG ZHANG, HAIHUA DENG, QIWEI LI, LIANHUI WANG, FANGYE LAO, JIAOLIANG LI, ZHONGHUA CHEN, HAINING CHEN, LIXING WENG, JINLING XU, WANKUAN SHEN
Принадлежит:

The invention relates to a rapid identification method of transgenic Bt sugarcane resistance to Stem Borers by artificial inoculating. The method includes planting sugarcane in buckets in greenhouse, choosing insect source, artificial inoculating and evaluation of resistance. The experimental insect source of the same batch transgenic Bt sugarcane strain is the newly hatched larvae of the same striped sugarcane borer, or equally divides the newly hatched larvae of 2-3 female moth born to each sugarcane to be analyzed. The result can be investigated after 5 days of artificial inoculating to the sugarcanes planted in buckets in greenhouse and the pest resistance of the sugarcane strain is evaluated comprehensively according to the larval survival rate and sugarcane damage level. The most notable characteristics of the invention are: 1. the consistency and unity of inoculating time are guaranteed to the utmost extent to ensure the accuracy of identification; 2. The periodicity of pest resistance ...

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Номер записи: 4
18-08-2010 дата публикации

Detoxication and tissue culture rapid propagation method of chewing cane axillary buds

Номер: CN0101803574A
Автор: YUEGUI CHEN, ZHONGHUA CHEN, RUI LIU, WANKUAN SHEN, JIANA TAN, ZHANDUAN YANG, CHEN YUEGUI, CHEN ZHONGHUA, LIU RUI, SHEN WANKUAN, TAN JIANA, YANG ZHANDUAN
Принадлежит:

The invention relates to the technical field of the detoxication and the tissue culture of sugarcane and provides a detoxication and tissue culture rapid propagation method of chewing cane, characterized by comprising the following steps of: (1) selecting and sterilizing plants; (2) carrying out the induced culture of axillary buds; (3) carrying out differentiation culture; (4) carrying out propagation culture; (5) carrying out rooting culture; (6) transplanting; and (7) detecting viruses (germs). The detoxication and tissue culture rapid propagation method of chewing cane is characterized in that a differentiation culture medium formula in the step (3) comprises the following components: 0.001-0.01mg/L of MS and TDZ, 0.02-0.05 mg/L of IBA, 30-40 g/L of cane sugar, 100-150 mg/L of arginine and 100-150 mg/L of inositol. The invention uses chewing cane axillary buds as explants, is easy to perform vaccination operation, and reaches the high survival rate over 90 percent. By the adopted differentiation ...

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Номер записи: 5
30-01-2008 дата публикации

Method for rapidly identifying resistance of transgenic Bt sugarcane to borer

Номер: CN0101113977A
Автор: DENG HAIHUA, WENG LIXING, LI JIAOLIANG, XU JINLING, LI QIWEI, WANG LIANHUI, ZHANG QIONG, CHEN ZHONGHUA, SHEN WANKUAN, CHEN HAINING, LAO FANGYE, YANG ZHANDUAN, HAIHUA DENG, LIXING WENG, JIAOLIANG LI, JINLING XU, QIWEI LI, LIANHUI WANG, QIONG ZHANG, ZHONGHUA CHEN, WANKUAN SHEN, HAINING CHEN, FANGYE LAO, ZHANDUAN YANG
Принадлежит:

The invention relates to a rapid identification method of transgenic Bt sugarcane resistance to Stem Borers by artificial inoculating. The method includes planting sugarcane in buckets in greenhouse, choosing insect source, artificial inoculating and evaluation of resistance. The experimental insect source of the same batch transgenic Bt sugarcane strain is the newly hatched larvae of the same striped sugarcane borer, or equally divides the newly hatched larvae of 2-3 female moth born to each sugarcane to be analyzed. The result can be investigated after 5 days of artificial inoculating to the sugarcanes planted in buckets in greenhouse and the pest resistance of the sugarcane strain is evaluated comprehensively according to the larval survival rate and sugarcane damage level. The most notable characteristics of the invention are: 1. the consistency and unity of inoculating time are guaranteed to the utmost extent to ensure the accuracy of identification; 2. The periodicity of pest resistance ...

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Номер записи: 6
01-02-2012 дата публикации

Detoxication and tissue culture rapid propagation method of chewing cane axillary buds

Номер: CN0101803574B
Автор: CHEN YUEGUI, CHEN ZHONGHUA, LIU RUI, SHEN WANKUAN, TAN JIANA, YANG ZHANDUAN, YUEGUI CHEN, ZHONGHUA CHEN, RUI LIU, WANKUAN SHEN, JIANA TAN, ZHANDUAN YANG
Принадлежит:

The invention relates to the technical field of the detoxication and the tissue culture of sugarcane and provides a detoxication and tissue culture rapid propagation method of chewing cane, characterized by comprising the following steps of: (1) selecting and sterilizing plants; (2) carrying out the induced culture of axillary buds; (3) carrying out differentiation culture; (4) carrying out propagation culture; (5) carrying out rooting culture; (6) transplanting; and (7) detecting viruses (germs). The detoxication and tissue culture rapid propagation method of chewing cane is characterized inthat a differentiation culture medium formula in the step (3) comprises the following components: 0.001-0.01mg/L of MS and TDZ, 0.02-0.05 mg/L of IBA, 30-40 g/L of cane sugar, 100-150 mg/L of arginine and 100-150 mg/L of inositol. The invention uses chewing cane axillary buds as explants, is easy to perform vaccination operation, and reaches the high survival rate over 90 percent. By the adopted differentiation ...

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Номер записи: 7
21-10-2009 дата публикации

Method for preparing germs polyclonal antibody of sugarcane ratoon stunting disease

Номер: CN0101560256A
Автор: CHEN JIANWEN, LIU RUI, QI YONGWEN, HE FANGYANG, WAN YUPING, YU HOUMEI, DENG HAIHUA, PAN YONGBAO, SHEN WANKUAN, YANG ZHANDUAN, JIANWEN CHEN, RUI LIU, YONGWEN QI, FANGYANG HE, YUPING WAN, HOUMEI YU, HAIHUA DENG, YONGBAO PAN, WANKUAN SHEN, ZHANDUAN YANG
Принадлежит:

The invention relates to the technical field for preventing and curing sugarcane ratoon stunting disease, providing a method for preparing germs polyclonal antibody of sugarcane ratoon stunting disease, comprising the following steps: (1) culturing pathogenic germs by means of isolation to obtain antigen; (2) mixing the antigen with equivalent Freund complete adjuvant, emulsifying, and immunizing rabbit; (3) mixing the antigen with equivalent Freund incomplete adjuvant, emulsifying, and again immunizing rabbit, repeating the immunizing process several times, after measuring of titer meets requirement, collecting blood from the heart, isolating antiserum; and (4) purifying the antiserum to obtain the polyclonal antibody. In the invention, the germs of the sugarcane perennial root dwarfing is isolated from sugarcane juice, thus the polyclonal antibody which is obtained thereby can specifically identify the germs of the sugarcane ratoon stunting disease in the sugarcane juice. The germs polyclonal ...

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Номер записи: 8
07-03-2012 дата публикации

Method for removing Leifsonia xyli subsp.xyli and quickly breeding healthy cane seedling

Номер: CN0101361459B
Автор: SHEN WANKUAN, YANG ZHANDUAN, LIU RUI, HE HUIYI, CHEN ZHONGHUA, DENG HAIHUA, WANKUAN SHEN, ZHANDUAN YANG, RUI LIU, HUIYI HE, ZHONGHUA CHEN, HAIHUA DENG
Принадлежит:

The invention relates to the propagation production of cane seedlings, and provides a method for removing cane perennial root dwarfing germs and rapidly propagating healthy cane seedlings, which comprises the following steps: little amount of original seeds are treated by hot water, stem tips which are detected to be feminine by the regenerated plantlet by carrying out speckle enzyme marker immune test (DB-EIA) is taken as an explant, healthy seedlings are produced by induction culture, secondary propagation culture, root-promoting culture, temporary planting and second-stage or third-stage nursery propagation-extension; furthermore, the disinfection of seed-cutting tools (cane knives) is paid attention to in the nursery propagation-extension, before the seedlings are used as seeds for large field production after coming out from nursery, cane stem juice is extracted by sampling, and RSD virus is detected by using the DB-EIA, thus guaranteeing the providing of qualified seedlings. Themethod ...

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Номер записи: 9