Настройки

Укажите год
-

Небесная энциклопедия

Космические корабли и станции, автоматические КА и методы их проектирования, бортовые комплексы управления, системы и средства жизнеобеспечения, особенности технологии производства ракетно-космических систем

Подробнее
-

Мониторинг СМИ

Мониторинг СМИ и социальных сетей. Сканирование интернета, новостных сайтов, специализированных контентных площадок на базе мессенджеров. Гибкие настройки фильтров и первоначальных источников.

Подробнее

Форма поиска

Поддерживает ввод нескольких поисковых фраз (по одной на строку). При поиске обеспечивает поддержку морфологии русского и английского языка
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Ведите корректный номера.
Укажите год
Укажите год
Применить Всего найдено 34. Отображено 34.
22-06-2017 дата публикации

SANDWICH ASSAYS IN DROPLETS

Номер: US20170176429A1
Автор: Michael L. Samuels, Darren Roy Link, SAMUELS MICHAEL L, LINK DARREN ROY, Samuels Michael L., Link Darren Roy
Принадлежит:

The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte. 165-. (canceled)66. A reagent library of a plurality of stabilized fluidic compartments in a container , comprising a first fluid compartment and a second fluid compartment , wherein the first fluid compartment comprisesa first binding agent specific for a first binding region on a first target analyte and comprising a first target identifier, anda second binding agent specific for a second binding region on the first target analyte, wherein the second binding agent comprises a functionalized portion for immobilization to a solid support;and wherein the second fluid compartment comprisesa third binding agent specific for a first binding region on a second target analyte and comprising a second target identifier, anda fourth binding agent specific for a second binding region on the second target analyte, wherein the fourth binding agent comprises a functionalized portion for immobilization to a solid support;wherein the first target identifier is different from the second target identifier.67. The reagent library of claim 66 , wherein each of the plurality of fluidic compartments is a droplet.68. The reagent library of claim 67 , wherein the droplet is surrounded by an immiscible carrier fluid.69. The reagent library of claim 66 , wherein the functionalized portion of the second binding agent is a terminal portion of the second binding agent.70. The reagent ...

Подробнее
Номер записи: 1
11-05-2017 дата публикации

ENZYME QUANTIFICATION

Номер: US20170131279A1
Автор: Darren Roy Link, Michael L. Samuels, LINK DARREN ROY, SAMUELS MICHAEL L, Link Darren Roy, Samuels Michael L.
Принадлежит:

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number. 124-. (canceled)26. The method of claim 25 , wherein the fluid droplets are surrounded by an immiscible carrier fluid.27. The method of claim 25 , wherein each of the fluid droplets is formed by merging a first fluid droplet comprising the particle with a second fluid droplet comprising the fluorescently labeled antibodies.28. The method of claim 25 , wherein in the flowing step claim 25 , the fluid droplets flow through a narrow channel in a second microfluidic channel and elongated.29. The method of claim 28 , wherein the localized signal is represented as a spike on a digital trace.30. The method of claim 25 , wherein the particle is a cell or a bead.31. The method of claim 25 , wherein the fluorescently labeled antibodies comprise a plurality of differently colored labels.32. The method of claim 31 , wherein the fluid droplets comprise multiple copies of a fluorescently labeled antibody that each includes a different fluorescent label.33. The method of claim 25 , wherein the signal is detected by a laser detector.34. The method of claim 25 , wherein each of the fluorescently labeled antibodies is immobilized on a solid support.35. The method of claim 25 , wherein the incubating step is carried out off-chip.36. The method of claim 25 , wherein the incubating step is carried out on-chip.37. The method of claim 25 , wherein the incubating step is carried out in a delay channel that is in fluidic communication with the first microfluidic channel. This application claims the benefit of U.S. Provisional Patent Application No. 61/492,602, ...

Подробнее
Номер записи: 2
31-01-2017 дата публикации

Enzyme quantification

Номер: US0009556470B2
Автор: Darren Link, Michael L. Samuels, LINK DARREN, SAMUELS MICHAEL L, Link Darren, Samuels Michael L.
Принадлежит: Raindance Technologies, Inc., LINK DARREN, SAMUELS MICHAEL L, RAINDANCE TECH INC, Link Darren, Samuels Michael L.

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

Подробнее
Номер записи: 3
03-02-2022 дата публикации

METHOD FOR DETECTING AND QUANTIFYING LATENT RETROVIRAL RNA SPECIES

Номер: US20220033919A1
Автор: Banerjee Sirshendu Roopom, Link Darren R., Samuels Michael L.
Принадлежит:

The invention includes methods for determining the presence of a latent viral population by analyzing an RNA population from the virus with digital techniques, such as digital PCR or by sequencing cDNA produced from the RNA. The invention additional includes methods for determining the presence of latent viral populations by detecting and/or quantifying enzymes that are uniquely associated with the virus, e.g., reverse transcriptases. 1. A method for detection of latent retrovirus , comprising the steps of:isolating a virion particle in an aqueous droplet surrounded by an immiscible fluid;lysing the virion particle in the aqueous droplet to release an RNA molecule;exposing the RNA molecule to at least one primer capable of hybridizing to at least a portion of the RNA molecule;synthesizing a cDNA product from the RNA molecule; anddetecting the cDNA product.2. The method of claim 1 , wherein detecting comprises amplifying the cDNA product to produce a plurality of substantially identical copies of the cDNA product.3. The method of claim 2 , further comprising sequencing the substantially identical copies of the cDNA product to produce sequence reads.4. The method of claim 2 , wherein the copies of cDNA product are detected with fluorescence detection.5. The method of claim 1 , wherein said detecting step comprises quantifying said cDNA.6. The method of claim 1 , further comprising introducing an amount of exogenous synthetic RNA claim 1 , DNA claim 1 , or mRNA.7. The method of claim 6 , further comprising normalizing the detected cDNA against the exogenous synthetic RNA claim 6 , DNA claim 6 , or mRNA.8. The method of claim 1 , further comprising collecting the virion particle from an isolated biological sample.9. The method of claim 8 , wherein the isolated biological sample is a T-cell.10. The method of claim 9 , wherein the T-cell is a CD4+ T cell.11. The method of claim 1 , wherein the latent retrovirus a human immunodefficiency virus (HIV).12. A method for ...

Подробнее
Номер записи: 4
10-02-2022 дата публикации

ENZYME QUANTIFICATION

Номер: US20220042995A1
Автор: Link Darren R., Samuels Michael L.
Принадлежит:

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number. 118-. (canceled)19. A method for detecting target molecules in a fluid sample , the method comprising:providing, to a microfluidic device, a fluid sample comprising target molecules, a plurality of beads each comprising a reactive component for binding a target molecule thereto, and a detection component for binding to a target molecule that is bound to a bead;partitioning, in the microfluidic device, the fluid sample into plurality of separate and isolated partitions of fluid; andmonitoring each of the plurality of partitions for detection of an event associated with contents of one or more of the plurality of partitions.20. The method of claim 19 , wherein the plurality of partitions comprises a first subset and a second subset claim 19 , wherein the first subset comprises partitions that each comprise no bead and the second subset comprises partitions that each comprise a single bead.21. The method of claim 20 , wherein a majority of the plurality of partitions are provided within either the first or the second subset.22. The method of claim 19 , wherein at least one of the plurality of partitions comprises an immunocomplex comprising a target molecule bound between a single bead claim 19 , via an associated reactive component claim 19 , and an associated detection component.23. The method of claim 22 , wherein the reactive component and the detection component comprise a first and a second antibody claim 22 , respectively.24. The method of claim 19 , wherein the detection component comprises one or more detectable labels.25. The method ...

Подробнее
Номер записи: 5
17-02-2022 дата публикации

DROPLET LIBRARIES

Номер: US20220047998A1
Автор: Hutchison John Brian, Link Darren Roy, Samuels Michael L., Weiner Michael
Принадлежит:

The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays. 114.-. (canceled)15. A method for detecting target molecules in a fluid sample , the method comprising:providing, to a microfluidic device, a fluid sample comprising target molecules, a plurality of beads each comprising a reactive component for binding a target molecule thereto, and a detection component for binding to a target molecule that is bound to a bead;partitioning, in the microfluidic device, the fluid sample into plurality of separate and isolated partitions of fluid, wherein at least one of the plurality of partitions comprises an immunocomplex comprising a target molecule bound between a single bead, via an associated reactive component, and an associated detection component; andmonitoring each of the plurality of partitions for detection of an event associated with contents of one or more of the plurality of partitions.16. The method of claim 15 , wherein the plurality of partitions comprises a first subset and a second subset claim 15 , wherein the first subset comprises partitions that each comprise no bead and the second subset comprises partitions that each comprise a single bead.17. The method of claim 16 , wherein a majority of the plurality of partitions are provided within either the first or the second subset.18. The method of claim 15 , wherein the reactive component and the detection component comprise a first and a second antibody claim 15 , respectively.19. The method of claim 15 , wherein the detection component comprises one or more detectable labels.20. The method of claim 19 , wherein the one or more detectable labels comprises at least one of an optical label claim 19 , an enzymatic label claim 19 , and a radioactive label.21. The method of claim 20 , wherein the one or ...

Подробнее
Номер записи: 6
17-02-2022 дата публикации

Enzyme quantification

Номер: US20220050108A1
Автор: Darren R. Link, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

Подробнее
Номер записи: 7
03-03-2022 дата публикации

COMPOSITIONS AND METHODS FOR MOLECULAR LABELING

Номер: US20220064711A1
Автор: Brown Keith, Link Darren R., Olson Jeffrey Charles, Samuels Michael L., Watson Andrew
Принадлежит:

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Подробнее
Номер записи: 8
25-03-2021 дата публикации

ENZYME QUANTIFICATION

Номер: US20210088519A1
Автор: Link Darren R., Samuels Michael L.
Принадлежит:

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number. 118-. (canceled)19. A method of performing an ELISA assay in droplets , the method comprising:providing reagents comprising reporter antibodies and beads linked to bead-bound antibodies;introducing a test fluid and forming ELISA sandwiches each comprising a target, one bead-bound antibody, a target analyte from the test fluid, and one reporter antibody;forming, via a microfluidic device, droplets wherein some of the droplets contain one of the ELISA sandwiches and a fluorogenic substrate; anddetecting fluorescence indicating presence of a target analyte in the test fluid.20. The method of claim 19 , wherein the reporter antibodies are provided as biotinylated antibodies.21. The method of claim 20 , further comprising introducing a streptavidin-linked enzyme for use in forming the ELISA sandwiches.22. The method of claim 21 , wherein streptavidin-linked enzyme comprises streptavidin beta-galactosidase.23. The method of claim 19 , wherein the fluorogenic substrate comprises fluorescein di-beta-D-galactopyranoside (FDG).24. The method of claim 19 , wherein the substrate is enzymatically turned-over to make a fluorescent product proportional to the concentration of the analyte.25. The method of claim 19 , wherein the target analyte comprises a protein.26. The method of claim 19 , wherein the detecting step comprises reading a first optical signal indicating the bead type and the fluorescence signal indicating the presence of the target analyte.27. The method of claim 19 , wherein the beads are magnetic.28. The method of wherein the droplets ...

Подробнее
Номер записи: 9
28-03-2019 дата публикации

ENZYME QUANTIFICATION

Номер: US20190094226A1
Автор: Link Darren Roy, Samuels Michael L.
Принадлежит:

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number. 124-. (canceled)25. A method for identifying components of a chemical reaction , the method comprising:forming a plurality of fluid partitions each comprising a particle that comprises a marker and a fluorescently labeled binder specific to the marker;incubating the fluid partitions, wherein the fluorescently labeled binder binds to the particle comprising the marker; anddetermining an amount of a localized signal from the labeled binder on the particle in the fluid partitions.26. The method of claim 25 , wherein the labeled binder is detected by an optical property.27. The method of claim 25 , wherein the determining step detecting a concentrated signal from the labeled binder localized to the particle compared to a dispersed signal from the labeled binder in the partition.28. The method of claim 25 , wherein said fluid partitions are droplets.29. The method of claim 28 , wherein the droplets are surrounded by an immiscible carrier fluid.30. The method of claim 25 , wherein determining the amount of the localized signal is based upon a ratio of a localized increase in signal intensity to partition wide decrease in signal intensity.31. The method of claim 25 , wherein the amount of labeled binder is determined by a signal strength measured in the fluid partitions.32. The method of claim 31 , wherein signal strength is measured by a laser.33. The method of claim 28 , wherein each of the droplets is formed by merging a first droplet comprising the particle with a second droplet comprising the labeled binder.34. The method of claim 33 , ...

Подробнее
Номер записи: 10
14-07-2016 дата публикации

COMPOSITIONS AND METHODS FOR MOLECULAR LABELING

Номер: US20160201125A1
Автор: Brown Keith, Link Darren R., Olson Jeffrey Charles, Samuels Michael L., Watson Andrew
Принадлежит:

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR. 131-. (canceled)32. A method of obtaining genetic sequence data for assembling a haplotype , the method comprising:forming a plurality of compartments, each compartment comprising a polynucleotide template and at least one primer for binding to the polynucleotide template, wherein the primer comprises a barcode and the barcodes differ between compartments;hybridizing the primers to the templates;extending the hybridized primers, whereby a plurality of extended primers comprising barcodes are produced; anddetermining the nucleic acid sequence of at least a portion of the extended primers, wherein the portion includes the barcodes.33. The method of claim 32 , wherein the compartment is a water-in-oil droplet.34. The method of claim 33 , wherein the primers are members of PCR primer pairs claim 33 , each primer pair having a first primer and a second primer.35. The method of claim 34 , wherein the both members of the PCR primer pair comprises a barcode.36. The method of claim 33 , wherein the compartments are formed by merging a droplet comprising the single copy of a polynucleotide template with a droplet comprising the at least one primer for binding to the polynucleotide template.37. The method of claim 32 , wherein each compartment comprises a plurality of different single copies of a polynucleotide template.38. The method of claim 32 , wherein the primers is each compartment comprise the same barcode within the same compartment.39. The method of claim 33 , wherein each primer pair target the amplification of a genomic region ...

Подробнее
Номер записи: 11
02-10-2014 дата публикации

Enzyme quantification

Номер: US20140295421A1
Автор: Darren Link, Michael L. Samuels
Принадлежит: Raindance Technologies Inc

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

Подробнее
Номер записи: 12
09-08-2018 дата публикации

DISTINGUISHING RARE VARIATIONS IN A NUCLEIC ACID SEQUENCE FROM A SAMPLE

Номер: US20180223348A1
Автор: Link Darren R., Samuels Michael L.
Принадлежит:

The invention generally relates to methods for distinguishing a rare genetic variation in a nucleic acid sequence. 126-. (canceled)27. A method comprising the steps of:producing a plurality of concatemer products each from a circularized nucleic acid molecule, wherein the concatemer products each comprises a repeating sequence element comprising a copy of the nucleic acid molecule and a unique sequence tag;compartmentalizing the concatemer products into compartmentalized portions, wherein a plurality of the compartmentalized portions comprise only a single concatemer product; andamplifying the concatemer product in the compartmentalized portions.28. The method according to claim 27 , wherein:the concatemer products are produced by a rolling circle reaction.29. The method according to claim 27 , further comprising:sequencing products of the amplifying step to produce a plurality of sequence reads.30. The method according to claim 29 , further comprising:analyzing the sequence reads to identify a variant from a consensus sequence in a plurality of the sequence reads that comprise a unique sequence tag sequence composition that is the same.31. The method according to claim 30 , wherein the variant is associated with a disease.32. The method according to claim 31 , wherein the disease is cancer.33. The method according to claim 29 , wherein prior to the sequencing step claim 29 , the method further comprises incorporating sequencing adaptors with the products of the amplifying step.34. The method according to claim 29 , wherein sequencing is sequencing-by-synthesis.35. The method according to claim 27 , wherein concatemer products are in an aqueous fluid and compartmentalizing comprises partitioning the aqueous fluid with an immiscible fluid to form droplets of the aqueous fluid.36. The method according to claim 35 , wherein the aqueous fluid is flowing in a channel and the produced droplets are flowing in a channel.37. The method according to claim 35 , wherein the ...

Подробнее
Номер записи: 13
16-07-2020 дата публикации

ENZYME QUANTIFICATION

Номер: US20200225232A1
Автор: Link Darren R., Samuels Michael L.
Принадлежит:

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number. 120-. (canceled)21. A method for detecting a condition in a human , the method comprising:forming fluid partitions comprising components of a chemical reaction;conducting said chemical reaction;determining a distribution of at least one product of said chemical reaction;comparing the distribution to an expected distribution of said product; andidentifying the presence of said condition if said distribution is statistically-significantly different than said expected distribution.22. The method of claim 21 , wherein said product is a protein.23. The method of claim 22 , wherein said protein is beta amyloid protein.24. The method of claim 23 , wherein said distribution is measured as an aggregate of said beta amyloid protein.25. The method of claim 21 , wherein at least one of the components of the chemical reaction comprises a detectable label that is acted on by the chemical reaction.26. The method of claim 25 , further comprising the step of identifying fluid partitions that contain released detectable label.27. The method of claim 25 , wherein the components comprise an enzyme and at least one substrate of the enzyme.28. The method of claim 27 , wherein the enzyme catalyzes a reaction that results in release of a detectable label from the substrate.29. The method of claim 28 , wherein the determining step comprises quantifying an amount of enzyme in the fluid partitions.30. The method of claim 29 , further comprising determining a number of enzyme molecules within each partition based upon signal strength of the detectable label.31. The ...

Подробнее
Номер записи: 14
06-08-2020 дата публикации

ENZYME QUANTIFICATION

Номер: US20200249230A1
Автор: Link Darren Roy, Samuels Michael L.
Принадлежит:

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the sunset. The amount of target can be determined based on the detected number. 1. A method for identifying components of a chemical reaction , the method comprising:forming fluid partitions comprising components of a chemical reaction, wherein at least one of said components comprises a detectable label that is acted on by said chemical reaction;conducting said chemical reaction;determining an amount of at least one of said components based upon one or more properties of the detectable label in said fluid partitions.2. The method of claim 1 , wherein the one or more properties of the detectable label includes amount of the detectable label.3. The method of claim 2 , wherein the amount of the detectable label is detected by an optical property.4. The method of claim 1 , further comprising the step of identifying fluid partitions that contain released detectable label.5. The method of claim 1 , wherein said components comprise an enzyme and at least one substrate of the enzyme.6. The method of claim 5 , wherein said enzyme catalyzes a reaction that results in release of the detectable label from said substrate.7. The method of claim 6 , wherein said determining step comprises quantifying an amount of enzyme in said fluid partitions.8. The method of claim 7 , further comprising determining a number of enzyme molecules within each partition based upon signal strength of the detectable label.9. The method according to claim 1 , wherein determining the amount of the least one of said components is based upon a localized concentration of the detectable label.10. The method according to claim 9 , wherein the localized ...

Подробнее
Номер записи: 15
27-08-2020 дата публикации

COMPOSITIONS AND METHODS FOR MOLECULAR LABELING

Номер: US20200270600A1
Автор: Brown Keith, Link Darren R., Olson Jeffrey Charles, Samuels Michael L., Watson Andrew
Принадлежит:

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR. 131.-. (canceled)32. A method for protein detection , the method comprising:introducing a protein-specific binding reagent to a sample comprising a population of cells;encapsulating the cells in droplets such that each droplet comprises a single cell and said protein-specific binding reagent; anddetermining the presence of proteins of said cells that are bound to said protein-specific binding reagents.33. The method of claim 32 , wherein said encapsulating step comprises merging droplets containing said cells and said protein-specific binding reagents.34. The method of claim 32 , further comprising the step of lysing said cells.35. The method of claim 32 , wherein said protein-specific binding reagent is selected from an antibody claim 32 , an aptamer claim 32 , a nucleic acid-labeled protein.36. The method of claim 32 , wherein the protein-specific binding reagents are coupled to beads.37. The method of claim 32 , wherein the protein-specific binding reagents are cleavable from the proteins to which they bind.38. The method of claim 32 , wherein the proteins are cell-surface proteins.39. The method of claim 32 , wherein the encapsulating step comprises loading the cells into a microfluidic chip.40. The method of claim 32 , wherein the protein-specific binding reagents further comprise a universal binding site.41. The method of claim 32 , further comprising quantifying the proteins.42. The method of claim 33 , wherein the droplets further comprise a library of universal barcodes coupled to said protein-specific binding ...

Подробнее
Номер записи: 16
23-12-2021 дата публикации

DISTINGUISHING RARE VARIATIONS IN A NUCLEIC ACID SEQUENCE FROM A SAMPLE

Номер: US20210395808A1
Автор: Link Darren R., Samuels Michael L.
Принадлежит:

The invention generally relates to methods for distinguishing a rare genetic variation in a nucleic acid sequence. 1. A method comprising the steps of:producing a first strand product from a forward strand of a nucleic acid molecule and a first strand product from a reverse strand of the nucleic acid molecule, wherein the first strand products from the forward strand and the reverse strand each comprise a unique sequence tag that comprises a sequence composition different from other unique sequence tags;compartmentalizing the first strand products from the forward strand and the reverse into compartmentalized portions, wherein a plurality of the compartmentalized portions comprise only a single first strand product; andamplifying the forward and reverse strand amplification products in the compartmentalized portions.2. The method according to claim 1 , further comprising:producing a plurality of first strand products from the forward strand and a plurality of first strand products from the reverse strand, wherein the first strand products from the forward strand and the reverse strand each comprise a unique sequence tag that comprises a sequence composition different from other unique sequence tags.3. The method according to claim 2 , wherein:the plurality of first strand products from the forward strand and the plurality of first strand products from the reverse strand, comprise products from a plurality of different loci4. The method according to claim 1 , wherein:the first strand products from the forward strand are produced in a first pool and the first strand products from the reverse strand are produced in a second pool.5. The method according to claim 1 , wherein:the amplification is an exponential amplification.6. The method according to claim 5 , wherein:the exponential amplification comprises PCR.7. The method according to claim 1 , wherein:the first strand products are produced by a polymerase extension reaction.8. The method according to claim 7 , ...

Подробнее
Номер записи: 17
17-10-2019 дата публикации

COMPOSITIONS AND METHODS FOR MOLECULAR LABELING

Номер: US20190316119A1
Автор: Brown Keith, Link Darren R., Olson Jeffrey Charles, Samuels Michael L., Watson Andrew
Принадлежит:

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR. 131-. (canceled)32. A method for analyzing a nucleic acid , the method comprising:obtaining nucleic acid from a cell or a population of cells;exposing the nucleic acid to a transposase to form a reaction product;introducing the reaction product to a droplet comprising barcoded primers and reagents for amplification;conducting an amplification reaction in the droplet to produce barcoded amplicons corresponding to transposase-accessible regions of the nucleic acid; andsequencing the transposase-accessible regions.33. The method of claim 32 , wherein the nucleic acid comprises genomic DNA.34. The method of claim 32 , wherein the exposing step results in reaction products in an aqueous suspension.35. The method of claim 32 , wherein the reaction product is a transposed nucleic acid.36. The method of claim 32 , wherein the introducing step comprises forming a plurality of droplets claim 32 , each comprising a different reaction product.37. The method of claim 32 , wherein the droplet comprises a single molecule of the reaction product.38. The method of claim 32 , wherein the droplet is formed by intersecting an aqueous fluid comprising the reaction product with a counter-propagating stream of oil.39. The method of claim 32 , wherein each of the barcoded primers comprises a functional N-mer portion and a unique N-mer portion.40. The method of claim 39 , wherein all unique N-mers within a droplet are identical.41. The method of claim 32 , wherein the barcoded primers hybridize at random locations on the reaction product.42. The ...

Подробнее
Номер записи: 18
13-12-2018 дата публикации

DROPLET LIBRARIES

Номер: US20180353913A1
Автор: Hutchison Brian, Link Darren Roy, Samuels Michael L., Weiner Michael
Принадлежит:

The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays. 114.-. (canceled)15. A method for detecting target molecules in a fluid sample , the method comprising:forming, in a microfluidic device, a plurality of separated partitions of fluid from a fluid sample comprising target molecules, wherein each of the plurality of partitions of fluid comprises a subvolume of the fluid sample, a single bead comprising a reactive component for binding a target molecule to the bead, and a detection component for binding to a target molecule bound to a bead to thereby form an immunocomplex, wherein the detection component comprises a detectable label; andmonitoring each of the plurality of partitions of fluid for detection of an event associated with contents of one or more of the plurality of partitions of fluid.16. The method of claim 15 , wherein the event comprises release of a detectable label in at least one of the plurality of partitions of fluid.176. The method of claim claim 15 , further comprising conducting a reaction in the at least one of the plurality of partitions of fluid claim 15 , wherein the reaction results in release of the detectable label.18. The method of claim 17 , wherein the reaction is an autocatalytic reaction.19. The method of claim 18 , wherein the autocatalytic reaction is a polymerase-chain reaction (PCR).20. The method of claim 17 , further comprising monitoring the reaction and detecting a signal from the released detectable label for determining the presence of target molecules.21. The method of claim 20 , wherein monitoring each of the plurality of partitions of fluid comprises measuring at least one property associated with one or more beads based on detection of signals from the one or more detectable labels with a detector.22. The ...

Подробнее
Номер записи: 19
24-12-2020 дата публикации

COMPOSITIONS AND METHODS FOR MOLECULAR LABELING

Номер: US20200399635A1
Автор: Brown Keith, Link Darren R., Olson Jeffrey Charles, Samuels Michael L., Watson Andrew
Принадлежит:

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR. 131.-. (canceled)32. A method for analyzing at least one protein associated with a cell comprising:segregating a single cell in a fluid compartment;providing at least one barcoded binder to the fluid compartment, wherein said barcoded binder comprises an oligonucleotide comprising a barcode sequence;allowing the barcoded binder to bind to a protein in the single cell;sequencing the barcode of the oligonucleotide; andidentifying the binder, thereby identifying the protein.33. The method of claim 32 , wherein prior to sequencing claim 32 , a barcode unique to the fluid compartment is attached to the barcode of the oligonucleotide thereby creating a composite barcode.34. The method of claim 32 , wherein sequencing comprises sequencing the composite barcode and identifying the composite barcode identifies the protein and the single cell.35. The method of claim 32 , wherein a plurality of the barcoded binders are provided to the at least one fluid compartment.36. The method of claim 35 , wherein each of a plurality of the barcoded binders attach to each of plurality of the protein of the single cell.37. The method of claim 36 , wherein identifying the binder comprises sequencing the barcodes and the number of barcodes sequenced quantitates the number of the protein of the single cell.38. The method of claim 32 , wherein a plurality of different barcoded binders are provided to the at least one fluid compartment claim 32 , wherein each different barcoded binder comprises a different barcode identifying each binder and each different ...

Подробнее
Номер записи: 20
16-11-2021 дата публикации

Distinguishing rare variations in a nucleic acid sequence from a sample

Номер: US11174509B2
Автор: Darren R. Link, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention generally relates to methods for distinguishing a rare genetic variation in a nucleic acid sequence.

Подробнее
Номер записи: 21
30-11-2021 дата публикации

Enzyme quantification

Номер: US11187702B2
Автор: Darren R. Link, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

Подробнее
Номер записи: 22
14-01-2020 дата публикации

Enzyme quantification

Номер: US10533998B2
Автор: Darren R. Link, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

Подробнее
Номер записи: 23
09-11-2021 дата публикации

Compositions and methods for molecular labeling

Номер: US11168353B2
Автор: Andrew Watson, Darren R. Link, Jeffrey Charles Olson, Keith Brown, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Подробнее
Номер записи: 24
07-12-2023 дата публикации

Enzyme quantification

Номер: US20230393069A1
Автор: Darren Roy Link, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

Подробнее
Номер записи: 25
16-07-2024 дата публикации

Enzyme quantification

Номер: US12038438B2
Автор: Darren R. Link, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

Подробнее
Номер записи: 26
26-09-2023 дата публикации

Compositions and methods for molecular labeling

Номер: US11768198B2
Автор: Andrew Watson, Darren R. Link, Jeffrey Charles Olson, Keith Brown, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Подробнее
Номер записи: 27
22-08-2024 дата публикации

Sandwich assays in droplets

Номер: US20240280569A1
Автор: Darren Roy Link, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

Подробнее
Номер записи: 28
13-07-2023 дата публикации

Droplet libraries

Номер: US20230219043A1
Автор: Darren Roy Link, John Brian Hutchison, Michael L. Samuels, Michael Weiner
Принадлежит: Bio Rad Laboratories Inc

The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

Подробнее
Номер записи: 29
21-12-2023 дата публикации

Sandwich assays in droplets

Номер: US20230408505A1
Автор: Darren Roy Link, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

Подробнее
Номер записи: 30
07-09-2023 дата публикации

Sandwich assays in droplets

Номер: US20230280337A1
Автор: Darren Roy Link, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

Подробнее
Номер записи: 31
02-04-2024 дата публикации

Sandwich assays in droplets

Номер: US11946929B2
Автор: Darren Roy Link, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.

Подробнее
Номер записи: 32
12-11-2024 дата публикации

Compositions and methods for molecular labeling

Номер: US12140590B2
Автор: Andrew Watson, Darren R. Link, Jeffrey Charles Olson, Keith Brown, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Подробнее
Номер записи: 33
12-11-2024 дата публикации

Compositions and methods for molecular labeling

Номер: US12140591B2
Автор: Andrew Watson, Darren R. Link, Jeffrey Charles Olson, Keith Brown, Michael L. Samuels
Принадлежит: Bio Rad Laboratories Inc

The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

Подробнее
Номер записи: 34