28-05-2008 дата публикации
Номер: CN0101187627A
Автор:
ZHAO YUQIANG,
ZHEN TIANMIN,
DENG XULI,
ZHAO FANG,
GONG MAOQING,
WANG HUAIWEI,
SUN CHUANHONG,
YUQIANG ZHAO,
TIANMIN ZHEN,
XULI DENG,
FANG ZHAO,
MAOQING GONG,
HUAIWEI WANG,
CHUANHONG SUN
Принадлежит:
The invention discloses a method for testing rapidly mosquito insecticide resistance, which comprises taking tinge culex sensitive strains and anti-dichlorvos, anti - propoxur strains, testing that employed mosquitoes are III age mosquito larvaes or vamatix mosquitoes , taking mosquito mixing slurry 100ul to drop into micro plate holes, adding lodized thioacetyl biliary lye 100ul in each hole, incubating for 30 minutes under 30 DEG C, adding additionally dithio double-pair nitro acid liquid 100ul in each hole, adding 0.001mmol/L insecticide 100ul in each hole after reacting for 5 minutes, reading OD values on an enzyme mark instrument under 410nm wavelength. A resisting mosquito is a mosquito whose OD value is greater than or equal to 0.6. The method of the invention certifies that testing results are accurate after trailing for many years, accuracy is to 99.5% , testing time is short, a large quantity of living insects aren't needed, testing cost is low, and momentary field tests and field ...
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