Biological marker-earthworm internal organ p450 content measuring method

The invention relates to cytochrome P450, specifically to a method for measuring the content of a biomarker-cytochrome P450 in earthworm viscera, which comprises the following steps of: (1) soaking a live earthworm in glycerol solution, and immediately dissecting and taking out the viscera thereof; (2) cleaning the viscera with cold saline water, transferring to a homogenization buffer, breaking cells with a glass tissue grinder, centrifuging with a ultra-speed centrifuge at low temperature (3DEG C) and 10,000g for 20min, centrifuging the supernatant at 150,000g for 90min, removing the supernatant, and collecting microsome sediment; and (3) dissolving the microsome sediment in a storage buffer, mixing, and measuring the total amount of cytochrome P450 and the protein content in the microsome suspension. The total amount of cytochrome P450 is quantified with sodium hydrosulfite-reduced CO difference spectra method. The invention has the advantages of simplicity, rapidness, low consumption ...

Experimental device for washing heavy metal pollution and method thereof

The invention relates to a leaching experimental device and the method of heavy metal pollution soil, the experimental device comprises: a leaching solution storage, a peristaltic pump, a leaching column group, a hose, a three-way glass tube, a hose clip and a filter liquor collector. The end of the hose arranged on the peristaltic pump input is immersed under the storage liquid level, the hose arranged on the output of the peristaltic pump is connected with the leaching column group; the leaching column group comprises a plurality of leaching columns, the leaching columns are connected with head and tail of each other through the hose and the three-way glass tube, the projecting end of the three glass tube is provided with a hose clip; the column of the leaching column is made of perspex tube, the upper and lower port of the perspex tube is respectively provided with a nylon membrane, an O-type pad and a button, a connecting hose is extracted in the centre of the button, the connecting ...

Underground sewage percolating and middle water reusing technology

Complex biological growth promoter used for recovering river bottom sediment

The invention relates to a complex biological growth promoter used for recovering river bottom sediment, which comprises the following components (by weight percent: 5 to 14 % of electrical stone, 85 to 94% of active zeolite, 0.3 to 1% of biochemical fulvic acids and 0.1 to 0.3% of CoCl2.6H2O, wherein natural electrical stone is used as the raw material of the electrical stone, and the natural electrical stone is crushed into super fine powder with the granularity of 800 to 1200 meshes; natural active zeolite is used as the raw material of the active zeolite, the natural active zeolite is firstly activated at the high temperature of 300 to 600 DEG C and then crushed into active zeolite powder with the granularity of 100 to 500 meshes; and the complex biological growth promoter used for recovering river bottom sediment can be obtained after the electrical stone, the active zeolite, the biochemical fulvic acids and the CoCl2.6H2O are dried, crushed and mixed. The invention has the characteristics ...

一种去除稠油采出水中COD的方法

The process of eliminating COD in water for thick oil production includes the first physical and chemical processing stage, the biological regulation stage and the second physical and chemical processing stage. The first physical and chemical processing stage is mainly oil-water separation to eliminate mineral oil and suspended matter in waste water greatly via conventional oil isolation, air floating and coagulation and to eliminate some COD; the biological regulation stage completes molecule reformation of organic pollutant to alter the chemical composition of COD via anaerobic processing,hydrolyzing and acidification and facultative biological processing; and the second physical and chemical processing stage eliminates COD in waste water via available coagulation and separation. Thesaid process can eliminate COD in water for thick oil production effectively.

Low concentration sewage anaerobic treatment process

The invention relates to a low-concentration domestic sewage anaerobic treatment method and an anaerobic treatment device, the anaerobic treatment method thereof is the two-stage composite anaerobic technology, the main reaction of the first stage is hydrolytic acidification, and the main indicators are controlled as follows: the hydraulic retention time (HRT) in a hydrolytic acidification reaction tank is to be 3.5 to 4.5 hours, the sludge concentration (MLSS) is to be 10 to 12g/L, and the reflux ratio (R) of mixed liquid is to be 1/10 to 1/8. The main reaction of the second stage is methanation, and the main indicators are controlled as follows: the hydraulic retention time (HRT) in the reaction tank is to be 4.0 to 5.5 hours, the sludge concentration (MLSS) is to be 13 to 15g/L, and thereflux ratio (R) of mixed liquid is to be 1/6 to 1/5. The treatment method can obtain the good domestic sewage treatment effect by the regulation of the hydraulic retention time, the sludge concentration ...

Method for distributing holes for subsurface infiltration sewage dispersion pipe

The invention discloses a method for distributing holes for a subsurface infiltration sewage dispersion pipe, which comprises the following steps of: opening a water inlet end of the sewage dispersion pipe, and closing the other end of the sewage dispersion pipe, wherein the length of the sewage dispersion pipe is 12 to 18 meters, and the diameter of the inner wall is 60 to 80mm; using the water inlet end of the sewage dispersion pipe as a starting point, uniformly drilling small holes with the diameter of 8mm according to the space between 8 and 10cm on the pipe wall of the front 4-6 meters,and uniformly drilling small holes with the diameter of 8mm according to the space between 6 and 8cm on the pipe wall of the rear 8-12 meters; drilling 3 small holes on the same circular section of the sewage dispersion pipe, taking the 90-degree sector of the circular section, drilling a small hole at two ends of the sector arc respectively, and drilling a small hole in the center of the arc; embedding ...

Experiment device for remediation of solid waste by anaerobes

The invention relates to an experiment device for remediation of solid waste by anaerobes. An inert gas cylinder is connected with an anaerobic generator by a flow pressure control instrument; the anaerobic generator is connected with a gas extraction pump by a switch valve through a hose; the gas extraction pump is connected with the hose which is directly guided into a gas collecting device; column bodies of the anaerobic generator are a plurality of isolating sieves; one side of each isolating sieve is provided with a sampling hole, and a semicircular base is arranged below the isolating sieve; and a semicircular top cover is arranged on the isolating sieves and is provided with a gas outlet hole and a nutrient adding pipe. In the equipment device, controllable anaerobic environment can be provided, acquisition and analysis of samples at different levels can be carried out, and nutrient regulation can be carried out at proper time according to the requirements.

Experimental device for washing heavy metal pollution

The invention relates to a leaching experimental device and the method of heavy metal pollution soil, the experimental device comprises: a leaching solution storage, a peristaltic pump, a leaching column group, a hose, a three-way glass tube, a hose clip and a filter liquor collector. The end of the hose arranged on the peristaltic pump input is immersed under the storage liquid level, the hose arranged on the output of the peristaltic pump is connected with the leaching column group; the leaching column group comprises a plurality of leaching columns, the leaching columns are connected with head and tail of each other through the hose and the three-way glass tube, the projecting end of the three glass tube is provided with a hose clip; the column of the leaching column is made of perspextube, the upper and lower port of the perspex tube is respectively provided with a nylon membrane, an O-type pad and a button, a connecting hose is extracted in the centre of the button, the connecting ...

Device for soil heavy metal adsorption /desorption kinetic procedure research

The invention relates to a research on soil heavy metal absorption/desorption action, in particular to a device used for researching the soil heavy metal absorption/desorption dynamic process, which is sequentially formed by a sampling liquid storage container, a multi-channel peristaltic pump, an exchange column and an effluent automatic adaptor which are connected in series by pipelines. The research can avoid the phenomenon of heavy metal re-absorption and make better simulation on natural conditions so as to research the soil heavy metal absorption/desorption dynamic process.

Culturing method of micrococcus for degrading nitrobenzene in river bottom mud

The invention provides a method for culturing a micrococcus strain capable of degrading nitrobenzene in bottom sediment of river course. Firstly, the invention designs an enrichment and separation medium for separating superior degradation bacteria of nitrobenzene in bottom sediment of river course. The specific steps comprise the following methods of: (1) preparing the enrichment and separation medium; (2) culturing the enrichment and separation culture medium; (3) preparing a pure medium; (4) culturing for purification; (5) preparing a medium for enlarging propagation; and (6) culturing for enlarging propagation. The invention can screen out the micrococcus with special degradation ability on nitrobenzene from the bottom sediment of river course for the first time. The screening, separation, purification and enlarged cultivation method of the micrococcus are simple and reliable, and the component of each medium can be easily obtained and conveniently prepared. The micrococcus can be directly ...

Unpowered reoxygenation water inflow system of subsurface flow artificial wetland in northern cold region

The invention discloses an unpowered reoxygenation water inflow system of a subsurface flow artificial wetland in a northern cold region. A water inflow pipe, a water inflow flange, a gas-liquid mixing chamber, a water effluent flange and a water outlet pipe are successively installed on the same centre line and are all buried below a soil permafrost; one end of the gas-liquid mixing chamber is connected with the water inflow pipe by the water inflow flange, and the other end of the gas-liquid mixing chamber is connected with the water outlet pipe by the water effluent flange; one end of an air suction pipe extends out of the ground surface and then is communicated with atmosphere, and the other end of the air suction pipe extends into the gas-liquid mixing chamber; only half pipe wall, which is adjacent to the side of the water inflow pipe, of the air suction pipe which extends into the gas-liquid mixing chamber is reserved, and the half pipe wall is called a negative-pressure baffle the ...

Preparation method of pH-responsive biological microcapsule

The invention relates to a preparation method of a pH-responsive biological microcapsule. A crosslinking technology is utilized to form a biological microcapsule in a semi-interpenetrating network structure of sodium alginate-chitosan-gelatin secondary complex coacervation product and polyvinyl pyrrolidone, and the volume of the biological microcapsule reversibly swells and shrinks as the pH value changes. The surface of the sodium alginate-chitosan-gelatin secondary complex coacervation product is modified by the polyvinyl pyrrolidone, so that the prepared biological microcapsule has high pH-responsiveness, excellent slow release performance and high denitrification efficiency.

Device for collecting root exudate in situ

The invention discloses a device for collecting plant root exudates in situ, comprising an incubator, a bracket, an illumination cultivation light, a straight glass tube, a peristaltic pump, a nutrients bottle, a resin column, a surge flask and a collection bottle, wherein, cultivation medium is arranged in the incubator, the outer surface of the incubator is covered with a plastic thin film, the upper end of the incubator is provided with a sealing board, and the side of the incubator is inserted with a thermometer; the lower end of the incubator is provided with a through hole which is sealed by a rubber plug, and glass wool is arranged on the rubber plug; the incubator is fixed by the bracket arranged on a base, an adjustable bracket is arranged on the bracket, and the upper end of the adjustable bracket is provided with the illumination cultivation light; a porous circular glass tube is arranged in the medium in the incubator, and the bottom of the porous circular glass tube is connected ...

Experimental device for collecting solid waste filtrate

The invention relates to an experimental device for collecting solid waste filtrate, wherein the lower end of a water tank is connected to a flow meter; the lower end of the flow meter is connected to a dividing tube through a main switch valve; the lower end of the dividing tube is equipped with a plurality of filtering tubes; the filtering tubes are connected to a filtering sprayer through a branch switch valve; an experimental table is placed under the filtering sprayer; a filtering hole is arranged on the experimental table corresponding to the filtering sprayer; a solid waste experimental box is placed on the filtering hole; a filtrate filtering device is mounted under the filtering hole on the experimental table; and the lower end of the filtrate filtering device is connected to a filtrate container through a plastic soft tube and the switch valve. Through the experimental device for collecting solid waste filtrate, the natural rainfall intensity can be imitated, sets of contrast ...

Method for distributing holes for subsurface infiltration sewage dispersion pipe

The invention discloses a method for distributing holes for a subsurface infiltration sewage dispersion pipe, which comprises the following steps of: opening a water inlet end of the sewage dispersion pipe, and closing the other end of the sewage dispersion pipe, wherein the length of the sewage dispersion pipe is 12 to 18 meters, and the diameter of the inner wall is 60 to 80mm; using the water inlet end of the sewage dispersion pipe as a starting point, uniformly drilling small holes with the diameter of 8mm according to the space between 8 and 10cm on the pipe wall of the front 4-6 meters, and uniformly drilling small holes with the diameter of 8mm according to the space between 6 and 8cm on the pipe wall of the rear 8-12 meters; drilling 3 small holes on the same circular section of the sewage dispersion pipe, taking the 90-degree sector of the circular section, drilling a small hole at two ends of the sector arc respectively, and drilling a small hole in the center of the arc; embedding ...

Water pollutant adsorption resin and preparation method thereof

The invention relates to a water pollutant adsorption resin which comprises the components (based on the weight percentage): 15-18wt% of water soluble thiacalixarene and 82-85wt% of anion resin. A preparation method of the water pollutant adsorption resin comprises: 1. the 82-85wt% of anion resin is weighed to be taken, washed by de-ionized water for three times, soaked in 5wt% of HCl solution for 3h, soaked in 5wt% of NaOH solution for 3h, rinsed by the de-ionized water until the water is neutral and then dried at 50 DEG C for standby application; 2. the 15-18wt% of water soluble thiacalixarene is weighed to be taken, dissolved in 200ml of de-ionized water and processed by ultrasonic oscillation for 1h, so that water soluble thiacalixarene solution can be obtained; 3. the dried anion resin is added into the water soluble thiacalixarene solution to be stirred at the constant temperature of 25 DEG C for 24h, and then the mixed solution is filtered, separated and rinsed by clear water to ...

Method for preparing alkali copper sulfate powder inhibiting activity of sulphate reducing bacteria

The invention relates to basic copper sulfate powder which can inhibit the activity of sulfate-reducing bacteria, the molecular formula thereof is Cu4(OH)6SO4, the basic copper sulfate powder is prepared by copper sulfate and basic solution, and a production method comprises the steps of: weighing, regulating the pH, stirring, basic titration, crystallization, aging, suction filtration at reducedpressure, two-stage drying and grinding. The basic copper sulfate powder is applicable to copper sulfate crystals of any type, the basic solution can contain NaOH, CaO, NH4OH, ammonium oxalate and ammonium acetate solution, the two-stage drying is adopted, the basic copper sulfate powder which can inhibit the activity of the sulfate-reducing bacteria can be produced without the high temperature calcination, and the product purity is very high. The basic copper sulfate powder has the advantages of higher inhibition efficiency of the sulfate-reducing bacteria, good thermal stability, difficult decomposition ...

Low concentration sewage anaerobic treatment process

The invention relates to a low-concentration domestic sewage anaerobic treatment method and an anaerobic treatment device, the anaerobic treatment method thereof is the two-stage composite anaerobic technology, the main reaction of the first stage is hydrolytic acidification, and the main indicators are as follows, the hydraulic retention time (HRT) in a hydrolytic acidification reaction tank is controlled to be 3.5 to 4.5 hours, the sludge concentration (MLSS) is 10 to 12g/L, and the reflux ratio (R) of mixed liquid is 1/10 to 1/8. The main reaction of the second stage is methanation, and the main indicators are as follows, the hydraulic retention time (HRT) in the reaction tank is controlled to be 4.0 to 5.5 hours, the sludge concentration (MLSS) is 13 to 15g/L, and the reflux ratio (R) of mixed liquid is 1/6 to 1/5. The treatment method can obtain the good domestic sewage treatment effect by the regulation of the hydraulic retention time, the sludge concentration, the nutrition allocated ...

Sewage disposal system for empty tower ecological aeration filter bed

The invention provides a sewage disposal system for an empty tower ecological aeration filter bed. In the system, a big filter bed is arranged; multiple filter material landfill areas and an empty tower aeration area are alternatively distributed to constitute the filter bed; one side of the filter bed is provided with a T-shaped water distribution pipe, and the other side of the filter bed is connected with a drainage canal through a water discharge pipe; the filter materials, such as grails, volcanic rocks, zeolites and the like, are filled in the filter material landfill areas; filter bed surface layer planting soil is laid on the top of the filer materials; wet land plants are planted on the filter bed surface layer planting soil; the pool walls of the filter material lanfill areas attwo side of the empty tower aeration area are perforated walls which are provided with a plurality of permeable holes; aeration pipes are installed at the bottom of the empty tower aeration area; theend ...

Underground sewage percolating and middle water reusing technology

The present invention relates to sewage treatment technology, and it features that domestic sewage is first treated in the pre-treating part comprising sewer well, grille well, setting tanks and pumping channel and then percolated in the percolating part comprising the first water distributing channel, the second distributing channel, water dispersing pipe and special soil. The percolated water through completely enclosed running is led to water collecting part comprising water collecting tank and centrifugal pumps and the water out of the centrifugal pumps can be used as middle water for various uses. The present invention adopts the in-situ sewage collecting, treating and reusing method, can save large amount of sewage conveying equipment and cost and save water resource. Building the underground system can protect ground environment.

Method for preparing exotrophic mycorrhizal fungi preparation for large waste land in mine area

The present invention relates to the method of exotrophic mycorrhizal fungi preparation for large waste land in mine area. Lignite coal in 20-80 wt% and one or several of turf, bran and saw dust in 20-80 wt% are mixed to prepare matrix, the matrix is further mixed with inorganic salt and partial nutrient elements and pressurized steam sterilized for 1-2 hr; mycorrhizal fungi thallus is produced via one-stage bilogical culture and two-stage biological fermentation and is inoculated in amount of 2-10% and in bacteria-free state into the matrix and constant temperature cultured. The present invention can regenerate and utilize coal mine energy source and provide effective technological way for restoring the biological state of coal mine waste land.

Sewage disposal system for empty tower ecological aeration filter bed

The invention provides a sewage disposal system for an empty tower ecological aeration filter bed. In the system, a big filter bed is arranged; multiple filter material landfill areas and an empty tower aeration area are alternatively distributed to constitute the filter bed; one side of the filter bed is provided with a T-shaped water distribution pipe, and the other side of the filter bed is connected with a drainage canal through a water discharge pipe; the filter materials, such as grails, volcanic rocks, zeolites and the like, are filled in the filter material landfill areas; filter bed surface layer planting soil is laid on the top of the filer materials; wet land plants are planted on the filter bed surface layer planting soil; the pool walls of the filter material lanfill areas at two side of the empty tower aeration area are perforated walls which are provided with a plurality of permeable holes; aeration pipes are installed at the bottom of the empty tower aeration area; the end ...

Method for elimination COD in water for thick oil production

Artificial soil, and formulating method and use thereof

The invention relates to ecological restoration, in particular to artificial soil and a preparation method thereof. The artificial soil consists of urban solid waste fly ash and sludge. The preparation method comprises the following steps: airing the fly ash and the sludge till water contents reach 1 to 5 percent and 55 to 60 percent of water content respectively, and mixing the fly ash and the sludge according to weight portion ratio of 3:1-1:2 to obtain the artificial soil. The preparation method realizes resource utilization of the urban solid waste; and the prepared artificial soil has better water retention and water storage performance, is close to meadow brown earth, has higher nutrient content at the same time, and has the heavy metal content far lower than national agricultural standard. The artificial soil can be used for replacing natural soil during the restoration of abandoned lands of mines. The preparation method realizes the resource utilization of the urban solid waste, ...

Method for preparing alkali copper sulfate powder inhibiting activity of sulphate reducing bacteria

The invention relates to basic copper sulfate powder which can inhibit the activity of sulfate-reducing bacteria, the molecular formula thereof is Cu4(OH)6SO4, the basic copper sulfate powder is prepared by copper sulfate and basic solution, and a production method comprises the steps of: weighing, regulating the pH, stirring, basic titration, crystallization, aging, suction filtration at reduced pressure, two-stage drying and grinding. The basic copper sulfate powder is applicable to copper sulfate crystals of any type, the basic solution can contain NaOH, CaO, NH4OH, ammonium oxalate and ammonium acetate solution, the two-stage drying is adopted, the basic copper sulfate powder which can inhibit the activity of the sulfate-reducing bacteria can be produced without the high temperature calcination, and the product purity is very high. The basic copper sulfate powder has the advantages of higher inhibition efficiency of the sulfate-reducing bacteria, good thermal stability, difficult decomposition ...

Device for collecting root exudate in situ

The invention discloses a device for collecting root exudates in situ, comprising an incubator, a bracket, an illumination cultivation light, a straight glass tube, a peristaltic pump, a nutrients bottle, a resin column, a surge flask and a collection bottle, wherein, cultivation medium is arranged in the incubator, the outer surface of the incubator is covered with a plastic thin film, the upper end of the incubator is provided with a sealing board, and the side of the incubator is inserted with a thermometer; the lower end of the incubator is provided with a through hole which is sealed by a rubber plug, and glass wool is arranged on the rubber plug; the incubator is fixed by the bracket arranged on a base, an adjustable bracket is arranged on the bracket, and the upper end of the adjustable bracket is provided with the illumination cultivation light; a porous circular glass tube is arranged in the medium in the incubator, and the bottom of the porous circular glass tube is connected ...

Experimental device for collecting solid waste filtrate

The invention relates to an experimental device for collecting solid waste filtrate, wherein the lower end of a water tank is connected to a flow meter; the lower end of the flow meter is connected to a dividing tube through a main switch valve; the lower end of the dividing tube is equipped with a plurality of filtering tubes; the filtering tubes are connected to a filtering sprayer through a branch switch valve; an experimental table is placed under the filtering sprayer; a filtering hole is arranged on the experimental table corresponding to the filtering sprayer; a solid waste experimental box is placed on the filtering hole; a filtrate filtering device is mounted under the filtering hole on the experimental table; and the lower end of the filtrate filtering device is connected to a filtrate container through a plastic soft tube and the switch valve. Through the experimental device for collecting solid waste filtrate, the natural rainfall intensity can be imitated, sets of contrast ...

Complex biological growth promoter used for recovering river bottom sediment

The invention relates to a complex biological growth promoter used for recovering river bottom sediment, which comprises the following components (by weight percent: 5 to 14 % of electrical stone, 85 to 94% of active zeolite, 0.3 to 1% of biochemical fulvic acids and 0.1 to 0.3% of CoCl2.6H2O, wherein natural electrical stone is used as the raw material of the electrical stone, and the natural electrical stone is crushed into super fine powder with the granularity of 800 to 1200 meshes; natural active zeolite is used as the raw material of the active zeolite, the natural active zeolite is firstly activated at the high temperature of 300 to 600 DEG C and then crushed into active zeolite powder with the granularity of 100 to 500 meshes; and the complex biological growth promoter used for recovering river bottom sediment can be obtained after the electrical stone, the active zeolite, the biochemical fulvic acids and the CoCl2.6H2O are dried, crushed and mixed. The invention has the characteristics ...