Labeling reagent

The current invention restates substituted nitroindole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted nitroindole nucleosides can be used as labeling reagents for the facile preparation of, e.g., optimized hybridization probes, simple probes, TAQMAN-probes or molecular beacon probes.

Method and device for stacking flat mail items

In a stacker, a mail item to be stacked is propelled against a stop wall at a stacking point by a stacking roll. A controlled driven underfloor belt and a stack support are displaced from the stack as the stack grows. The mail item to be stacked meets the preceding, already stacked item at an acute angle. Before it is stacked the mass of each item is determined. Stacking forces are measured with a measurement device in a preceding test phase the target values for the stacking pressures and the braking curve of the stacking roll up until the mail items have reached the stop are defined and stored based on statistical analyses in accordance with the mass of the mail items to be stacked. The speed of the respective mail item is reduced according to the braking curve assigned to the determined mass of the mail item.

MEMS SENSOR

A MEMS sensor ( 1 ) comprises a MEMS transducer ( 10 ) being coupled to a MEMS interface circuit ( 20 ). The MEMS interface circuit ( 20 ) comprises a bias voltage generator ( 100 ), a differential amplifier ( 200 ), a capacitor ( 300 ) and a feedback control circuit ( 400 ). The bias voltage generator ( 100 ) generates a bias voltage (Vbias) for operating the MEMS transducer. The variable capacitor ( 300 ) is connected to one of the input nodes (I 200 a ) of the differential amplifier ( 200 ). At least one of the output nodes (A 200 a , A 200 b ) of the differential amplifier is coupled to a base terminal (T 110 ) of an output filter ( 110 ) of the bias voltage generator ( 100 ). Any disturbing signal from the bias voltage generator ( 100 ) is a common-mode signal that is divided equally on the input nodes (I 200 a , I 200 b ) of the differential amplifier ( 200 ) and is therefore rejected.

Active material emitting device

An active material cartridge comprises a frame and an active material refill. The active material refill comprises at least one reservoir having an active material therein and a protrusion extending from a first end thereof. The active material refill is disposed on and attached to the frame.

Method for calibrating an ultrasound flowmeter

A method for the dry calibration of an ultrasonic flow measuring device, wherein, on the basis of predetermined, geometric, production data of the flow measuring device, information concerning the theoretical flow of the medium through a measuring tube is won, actual geometric measurement data of the flow measuring device is three-dimensionally determined, on the basis of the actual geometric measurement data, information concerning the actual flow of the medium through the flow measuring device is won, and, on the basis of the information regarding the theoretical flow, and the actual flow, of the medium through the flow measuring device, a correction, or calibration, factor is determined for the flow measuring device.

Signal processing arrangement, sensor arrangement and signal processing method

A signal processing arrangement has a signal input for connecting a capacitive sensor. An amplifier circuit is coupled between the signal input and a feedback point. A loop filter is coupled downstream to the feedback point. A quantizer is connected downstream to the loop filter and provides a multi-bit output word. The multi-bit output word consists of one or more higher significance bits and one or more lower significance bits. A first feedback path is coupled between a quantizer and the feedback point for providing a first feedback signal to the feedback point being representative of the one or more lower significance bits. A second feedback path is coupled to the quantizer for providing a second feedback signal to the signal input being representative of the one or more higher significance bits.

METHOD OF IMMOBILIZING A CELL ON A SUPPORT USING COMPOUNDS COMPRISING A POLYETHYLENE GLYCOL MOIETY

The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support. 1. A method of immobilizing a cell on a support , the method comprising wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and', 'wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and', 'wherein the compound comprises a linking group;, 'a) providing a compound or salt thereof comprising one or more hydrophobic domains attached to a hydrophilic domain,'}b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; andc) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.2. The method of claim 1 , wherein the compound comprises one or more hydrophobic domains and a hydrophilic domain claim 1 ,wherein the one or more hydrophobic domains are ...

Printed Circuit Board

The invention relates to a printed circuit board (60) having an insulating support layer (61) and a layer (62) of conductive material applied thereto. In order to increase the flexibility of the printed circuit board (60), it is proposed that material from the support layer (61) have been removed at least one point on the printed circuit board (60) on a side of the support layer (61) which is opposite the layer (62) of conductive material in order to form a straight groove (63) which extends from one side of the printed circuit board (60) to another side of the printed circuit board (60), so that the printed circuit board (60) can be bent along the groove (63), wherein the material of the support layer (61) which has remained in the region of the groove (63) and/or the layer (62) of conductive material in the region of the groove (63) form(s) a bending edge (64).

USE OF COMPOUNDS COMPRISING TWO OR MORE HYDROPHOBIC DOMAINS AND A HYDROPHILIC DOMAIN COMPRISING PEG MOIETIES FOR STABILIZATION OF A CELL

The present invention relates to the use compounds comprising two or more hydrophobic domains and a hydrophilic domain comprising a polyethylene glycol (PEG) moiety for stabilization of a cell, and methods related thereto. 1. A method comprising:providing of a compound comprising two or more hydrophobic domains attached to a hydrophilic domain, wherein the two or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the two or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, andcontacting said compound to a cell, wherein said contacting results in stabilization of the cell.2. The method of claim 1 , wherein the compound comprises two or more hydrophobic domains and a hydrophilic domain claim 1 ,wherein the two or more hydrophobic domains are covalently bound to said hydrophilic domain, andwherein the two or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and {'br': None, 'sub': n', 'k1', 'n', 'k2, 'X1-[A1-(L1)]-Z-[A2-(L1)]-X2 \u2003\u2003(I),'}, 'wherein the hydrophilic domain comprises a compound of Formula (I)wherein{'sub': 2', '2, 'Z is linear polyethylene glycol (PEG) moiety containing 1 to 100, wherein the polyethylene glycol moiety optionally comprises 1 or more spacer moieties SP connecting two —O—CH—CH— moieties, and wherein the linear PEG moiety optionally comprises a linker moiety L3 at one or both ends,'}each L1 is a linker moiety selected independently from each other,each n is either 0 or 1, selected independently from each other,A1 and A2 are bifunctional or trifunctional moieties selected independently from each other, and at least one A1 or A2 is trifunctional,k1 and k2 are integers between 0 and 10, selected independently from each other, and at least one of k1 and k2 is not 0,X1 and X2 are independently selected from hydrogen or a protecting group,L3 is ...

Lighting device for a motor vehicle

The invention relates to a lighting device for a motor vehicle. Said lighting device comprises a gas discharge lamp ( 1; 96 ) for emitting light, a starting device for providing a starting voltage for starting the gas discharge lamp ( 1; 96 ) and a control device for providing an input voltage for the starting device and an operating voltage for operation of the gas discharge lamp ( 1; 96 ). The control device is an integral part of the starting device. In order to come to grips with the problems associated with space and temperature with such lighting devices, the invention proposes that the combined starting and control device ( 5; 80 ) has a plug element ( 6 ) for connecting a vehicle power supply system voltage ( 7 ), the plug element ( 6 ) being in the form of a printed circuit board plug, which is formed by conductor tracks ( 21; 23 ) guided at an edge of a printed circuit board ( 20 ) of the starting and control device ( 5; 80 ).

Analog-to-digital converter system, electronic device and analog-to-digital conversion method

An ADC system comprises a coarse ADC for determining a coarse word representing an input signal, and an incremental ADC for determining a fine word based on a combination of the input signal and a feedback signal. A first combiner generates a first intermediate output word by joining the coarse word and the fine word. A feedback path generates the feedback signal based on the first intermediate output word. A decimation filter generates a second intermediate output word by filtering the first intermediate output word. A correction block determines a correction word based on the coarse word, on the first and the second predetermined number of bits and conversion parameters of the incremental ADC. A second combiner generates an output word by addition of the second intermediate output word and the correction word.

Device providing coordinated emission of light and volatile active

A flameless candle that releases a volatile active includes at least one LED positioned in a tip (106), a cartridge mount (128), and a support structure (102, 141). The at least one LED emits a flickering light that emulates a flame of a candle. The cartridge mount (128) receives and secures a replaceable cartridge (104a) containing a volatile active to be released into the atmosphere over time. The support structure (120, 141) supports the at least one LED and the cartridge mount (128). The support structure (120, 141) is configured to allow airflow across the replaceable cartridge (104a) when the replaceable cartridge (104a) is mounted in the cartridge mount (128).

Method for monitoring an ultrasound flowmeter system and/or process

A method for system- and/or process-monitoring in the case of an ultrasound flow measuring device having a plurality of measuring channels, wherein a medium flows though a pipeline or measuring tube in the direction of a longitudinal axis of the pipeline or measuring tube. Ultrasonic measuring signals are coupled into the pipeline or measuring tube and coupled out of the pipeline or measuring tube in such a way that they travel through the medium along different sound paths. Following passage through the pipeline or measuring tube, the ultrasonic measuring signals are detected in the individual measuring channels, wherein at least one actual quantity describing the ultrasonic measuring signal of each measuring channel is ascertained. The actual quantity is compared with a desired quantity captured under predetermined system- or process-conditions, and a report is output in the case of a difference between the ascertained, actual quantity and the captured, desired quantity.

LOW-PASS FILTER ARRANGEMENT

In an embodiment a low-pass filter arrangement has an input terminal for receiving an input voltage, a first voltage source coupled to the input terminal, a serial connection comprising a first and a second filter diode, the serial connection being coupled to the first voltage source, wherein a connection point between the first and the second filter diode is coupled to an output terminal of the filter arrangement, and a first filter capacitor coupled between the output terminal and a filter reference potential terminal. Therein the first voltage source is adapted to provide a first adjustable forward voltage whereby the first and the second filter diodes are both biased in a forward direction. 1. A low-pass filter arrangement havingan input terminal for receiving an input voltage (Vin),a first voltage source coupled to the input terminal,serial connection comprising a first and a second filter diode, the serial connection being coupled to the first voltage source, wherein a connection point between the first and the second filter diode is coupled to an output terminal of the filter arrangement,a first filter capacitor coupled between the output terminal and a filter reference potential terminal,wherein the first voltage source is adapted to provide a first adjustable forward voltage whereby the first and the second filter diodes are both biased in a forward direction.2. The low-pass filter arrangement according to claim 1 ,wherein the first voltage source comprises a first current source, a second current source, a resistor, a first and a second replica diode,wherein the first current source is coupled to a supply potential terminal and in series to a serial connection consisting of the first and the second replica diode, said serial connection in turn being coupled to a generation reference potential terminal and a connection point between the first current source and said serial connection forms a first output of the first voltage source,wherein the second ...

Microfluidic Chip, Device and System for the Generation of Aqueous Droplets in Emulsion Oil for Nucleic Acid Amplification

A microfluidic chip, device, system, the use thereof and method for the generation of aqueous droplets in emulsion oil for nucleic acid amplification. 1. A microfluidic chip for the generation of aqueous droplets for nucleic acid amplification , said microfluidic chip comprising:a. a first reservoir for uptake of a hydrophobic liquid ending in at least one inlet or at least one inlet coming from an external reservoir of hydrophobic liquid, each inlet ending into a first microfluidic channel connecting the at least one first reservoir with a reaction vessel,b. at least one element comprising a second reservoir for uptake of a hydrophilic solution ending in a vertical inlet into a second microfluidic channel connecting the second reservoir with a first microfluidic channel,c. at least one T-junction between said first and second microfluidic channel, characterized in that the end of said second microfluidic channel is joined radial and horizontal to the first microfluidic channel.2. The microfluidic chip of claim 1 , wherein said end of the second channel is joined to the first channel perpendicularly.3. The microfluidic chip of claim 1 , wherein said first reservoir for uptake of a hydrophobic liquid ends in at least one vertical inlet or in at least one horizontal inlet.4. The microfluidic chip of claim 1 , wherein said hydrophilic solution is an aqueous mixture comprising a nucleic acid sample and wherein said hydrophobic liquid comprises an emulsion oil or a mixture of emulsion oils and optionally a surfactant.5. A device for the generation of aqueous droplets for nucleic acid amplification claim 1 , the device comprising a microfluidic chip according to claim 1 , first means for providing a flow within said first microfluidic channel and second means for providing a flow within said second microfluidic channel.6. The device of further comprising means for holding at least one reaction vessel for collecting the aqueous droplets claim 5 , wherein the at least one ...

Active material emitting device

An active material cartridge comprises a frame and an active material refill. The active material refill comprises at least one reservoir having an active material therein and a protrusion extending from a first end thereof. The active material refill is disposed on and attached to the frame.

SENSORS WITH CORRUGATED DIAPHRAGMS

A sensor includes a substrate; and a corrugated diaphragm offset from the substrate. The corrugated diaphragm is configured to deflect responsive to a sound wave impinging on the corrugated diaphragm. A cavity is defined between the corrugated diaphragm and the substrate, the corrugated diaphragm forming a top surface of the cavity and the substrate forming a bottom surface of the cavity. A pressure in the cavity is lower than a pressure outside of the cavity. 139-. (canceled)40. A sensor comprising:a substrate; anda corrugated diaphragm offset from the substrate, the corrugated diaphragm being configured to deflect responsive to a sound wave impinging on the corrugated diaphragm; andin which a cavity is defined between the corrugated diaphragm and the substrate, the corrugated diaphragm forming a top surface of the cavity and the substrate forming a bottom surface of the cavity, in which a pressure in the cavity is lower than a pressure outside of the cavity.41. The sensor of claim 40 , in which the corrugated diaphragm comprises one or more of:a membrane;a plate; and/ora conductive diaphragm.42. The sensor of claim 40 , comprising circuitry configured to enable generation of an electrical signal based on the deflection of the corrugated diaphragm.43. The sensor of claim 40 , in which the corrugated diaphragm comprises a conductive diaphragm and the substrate comprises an electrode.44. The sensor of claim 43 , comprising circuitry configured to enable generation of an electrical signal based on a voltage between the corrugated diaphragm and the electrode of the substrate.45. The sensor of claim 44 , comprising a voltage source configured to apply a bias voltage between the diaphragm and the electrode of the substrate.46. The sensor of claim 40 , in which a surface of the corrugated diaphragm facing the substrate is reflective.47. The sensor of claim 46 , in which the substrate comprises:a light source positioned to illuminate the reflective surface of the ...

Compounds comprising one or more hydrophobic domains and a hydrophilic domain comprising peg moieties, useful for binding cells

The present invention relates to novel compounds comprising one or more hydrophobic domains and a hydrophilic domain comprising PEG moieties, useful for binding cells, as well as uses and compositions related thereto. The compounds are useful for immobilizing and/or stabilizing cells.

USE OF A REAGENT FOR THE LYSIS OF ERYTHROCYTES AS WELL AS METHODS AND KITS RELATING THERETO

The present disclosure relates to use of a reagent for the lysis of erythrocytes, a method of lysing erythrocytes and a kit comprising the reagent.

Split signal differential MEMS microphone

A microphone assembly includes a capacitive MEMS transducer and a bias circuit having a DC output coupled to the transducer. The microphone assembly also includes a differential amplifier having a first input coupled to a first output of the transducer, the differential amplifier having a second input coupled to a second output of the transducer. The microphone assembly further includes a first impedance matching network and a second impedance matching network configured to balance a first capacitive load at the first input of the amplifier and a second capacitive load at the second input of the amplifier. Electrical signals applied by the transducer to the first and second inputs of the amplifier are matched or approximately matched in magnitude and 180 degrees or approximately 180 degrees out of phase with respect to each other.

CLONAL PRE-AMPLIFICATION IN EMULSION

Disclosed is a process for clonal pre-amplification of a nucleic acid involving the steps of (i) providing a plurality of different nucleic acid molecules (b) attaching adaptor sequences to the 3′ ends and 5′ ends of the nucleic acid molecules (c) preparing a water in oil emulsion wherein the majority of water droplets comprises one or none member of the plurality of different nucleic acid molecules (d) clonally amplifying the plurality of different nucleic acid molecules. In particular, the different nucleic acid molecules are mRNA molecules. 1. A method comprising the steps of(a) providing a plurality of different nucleic acid molecules,(b) attaching adaptor sequences to the 3′ ends and 5′ ends of the nucleic acid molecules,(c) preparing a water in oil emulsion wherein a majority of water droplets comprises one or none of the plurality of different nucleic acid molecules, and(d) clonally amplifying the plurality of different nucleic acid molecules.2. The method according to claim 1 , wherein the different nucleic acid molecules are single stranded molecules.3. The method according to claim 1 , wherein the different nucleic acid molecules are RNA molecules.4. The method according to claim 1 , wherein the different nucleic acid molecules are polyadenylated RNA molecules.5. The method according to claim 1 , wherein the different nucleic acid molecules are mRNA molecules.6. The method according to claim 1 , further comprising the steps of a 5′ terminal part representing a primer binding site, and', 'a 3′ terminal part which is either an oligo dT sequence of at least 5 nucleotides in length, an essentially randomized sequence of at least 5 nucleotides in length, or a gene family specific sequence,, '(b1) hybridizing a first single stranded adaptor nucleic acid molecule to the plurality of different nucleic acid molecules, the adapter molecule comprising'}(b2) performing a first strand cDNA synthesis in the presence an RNA dependent DNA polymerase and a dNTP mixture in ...

Integrated instrument performing synthesis and amplification, and a system and method thereof

An integrated instrument for oligonucleotide synthesis and PCR, and a system and method thereof are disclosed. The integrated instrument is basically composed of two independent modules. The first module is a unit for chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample to be analyzed into the integrated instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.

Device for positioning a clamp-on flowmeter on a container

An apparatus for the positioning of a clamp-on flow measuring device on a containment, which has a medium flowing through it. The apparatus includes at least two ultrasonic transducers, which emit measuring signals into the containment and/or receive measuring signals from the containment, a positioning unit for the variable positioning of the ultrasonic transducers on the containment, a control/evaluation unit, which calculates from predetermined process and/or system variables a characteristic, desired variable of the measuring signals and which determines on the basis of a comparison of the calculated, desired variable with the corresponding, measured, actual variable, whether the ultrasonic transducers are optimally positioned or whether the position of the ultrasonic transducers need to be changed, and an indicating unit, which indicates to operating personnel a required change of position and/or the direction in which a change of position needs to be made.

Miniaturized, high-throughput nucleic acid analysis

The present invention is directed to method for analyzing multiple nucleic acid molecules of interest comprising in the steps of (i) providing a plurality of beads, characterized in that each bead comprises at least two sequence specific amplification primers, further characterized in that at least one of the primers is bound to the bead via a cleavable linker, (ii) capturing the nucleic acid molecules of interest from a sample, (iii) clonally isolating the plurality of beads, (iv) cleaving the at least one primer, (v) clonally amplifying the nucleic acid thereby creating multiple amplification products, and (vi) analyzing the amplification products.

Balance having a load changing device and method for operating said balance

A balance with a load changing device (14) that has a magazine table, mounted to be horizontally movable and having a plurality of vertical openings (26) arranged in groups. The balance (12) has a load cell (34) and a load receptor (42) with a carrier arrangement (44) corresponding to each of the groups of openings. The magazine table and the balance are height adjustable relative to each other. In a weighing position, a group of openings above the load receptor is penetrated by the carrier arrangement. In a changing position, the carrier arrangement is positioned lower than the openings in the magazine table. The magazine table is mounted at a fixed height relative to a base (16), and the balance is height adjustable relative to the base. The travel of the balance is upwardly limited by a stop (32), which is at a fixed height relative to the base.

SPLIT SIGNAL DIFFERENTIAL MEMS MICROPHONE

An integrated circuit includes a first amplifier and a second amplifier. A first impedance matching circuit is coupled to the first amplifier, a first charge pump, and a single MEMS transducer. A second impedance matching circuit is coupled to the second amplifier, a second charge pump, and to the single MEMS transducer. A first capacitive load as measured at an input of first amplifier, and a second capacitive load as measured at an input of the second amplifier exist. The first capacitive load and the second capacitive load are balanced with respect to each other. A single pressure change causes the single MEMS transducer to create a first electrical signal and a second electrical signal. Both the first electrical signal and the second electrical signal are matched or approximately matched in magnitude, and 180 degrees or approximately 180 degrees out of phase with respect to each other. 1. A microphone assembly comprising:a capacitive transducer;a bias circuit having a DC output coupled to the transducer;a differential circuit having a first input coupled to a first output of the transducer and a second input coupled to a second output of the transducer;an impedance matching network coupled to the first and second inputs of the differential circuit,the impedance matching network configured to balance a first load at the first input of the differential circuit and a second load at the second input of the differential circuit,wherein electrical signals applied by the transducer to the first and second inputs of the differential circuit are matched or approximately matched in magnitude, and the electrical signals are 180 degrees or approximately 180 degrees out of phase with respect to each other.2. The microphone assembly of claim 1 , wherein the bias circuit includes a first charge pump coupled to a first contact of the transducer and a second charge pump coupled to a second contact of the transducer.3. The microphone assembly of claim 1 , wherein the bias circuit ...

Control and an integrated circuit for a multisensory apparatus

A control for a multisensory apparatus comprises means for detecting a number of lights connected to the control. The control further includes means responsive to the detecting means for operating the light(s) connected to the control in either of first and second different modes of operation in dependence upon the detected number of lights connected thereto. Still further, the control includes means for actuating an active material dispenser.

Method and device for determining and/or monitoring a volume and/or mass flow

A method and a device for determining and/or monitoring the volume flow rate and/or mass flow rate of a medium flowing through a container. In the case of the device, such is an ultrasonic flow measuring device, which works on the basis of the travel-time-difference principle. Additionally, a technique is presented whereby, on the basis of the results of measurement, also information is provided concerning change in some other system and/or process variable. To this end, the currently measured, actual measuring signals, or the corresponding actual measuring data, is compared with corresponding, stored set measuring signals, or set measuring data; a report is output, when a deviation arises between the set measuring signals, or set measuring data, and the actual measuring signals, or actual measuring data.

Method of immobilizing a cell on a support using compounds comprising a polyethylene glycol moiety

The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

Microfluidic chip, device and system for the generation of aqueous droplets in emulsion oil for nucleic acid amplification

A microfluidic chip, device, system, the use thereof and method for the generation of aqueous droplets in emulsion oil for nucleic acid amplification.

Clonal pre-amplification in emulsion

Disclosed is a process for clonal pre-amplification of a nucleic acid involving the steps of (i) providing a plurality of different nucleic acid molecules (b) attaching adaptor sequences to the 3′ ends and 5′ ends of the nucleic acid molecules (c) preparing a water in oil emulsion wherein the majority of water droplets comprises one or none member of the plurality of different nucleic acid molecules (d) clonally amplifying the plurality of different nucleic acid molecules. In particular, the different nucleic acid molecules are mRNA molecules.

Method of immobilizing a cell on a support using compounds comprising a polyethylene glycol moiety

The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

METHODS OF SPECIFICALLY RELEASING A SUB-GROUP OF OBJECTS

The present invention relates to a method of specifically releasing a one or more members of a sub-group of objects from an entity, a method of detecting a subject's disease by detecting one or more members of a sub-group of biological entities indicative of the disease and a method of isolating one or more members of a sub-group of objects from a group of objects.

Assembly for detecting electromagnetic radiation and method of producing an assembly for detecting electromagnetic radiation

The assembly comprises a semiconductor device with an active-pixel array, a readout circuit chip or plurality of readout circuit chips mounted outside the active-pixel array, the readout circuit chip or plurality of readout circuit chips being configured to read out voltages or currents provided by the active-pixel array, and electric connections between the active-pixel array and the readout circuit chip or plurality of readout circuit chips.

Hydraulic flushing valve arrangement

A hydraulic flushing valve arrangement includes a flushing valve having a housing, flushing piston, and at least one spring. The housing includes a valve bore, two circular housing control edges, two inlet channels fluidically connected to the bore, and an outlet channel opening into the bore between the inlet channels. The piston includes two control collars, a piston neck, and piston control edges that delimit the collars from the neck, and that have circular shapes with a recess. The recess has a portion that, in a cross-sectional plane perpendicular to an axis of the piston, is constant and sized such that a flushing quantity is reduced when the portion of the recess is situated in a region of a corresponding housing control edge relative to a quantity when the piston is in an end position. The collars guide the piston so as to be longitudinally displaceable in the bore, and are spaced apart by the neck. The piston and control edges are configured to control the fluidic connections ...

Solid support for high-throughput nucleic acid analysis

The present invention provides a solid support which is preferably a bead comprising at least two sequence specific amplification primers wherein at least one primer is bound to the support with an inducible cleavable linker. The present invention also provides various method for preparing a solid support comprising at least two sequence specific primers, further characterized in that at least one of the primers is cleavable.

Amplifier arrangement and amplification method

An amplifier arrangement has an input transistor being connected between reference potential terminals by a current source and a current sink. An amplifier stage has an amplifier output coupled to a first connection node between the current sink and a first terminal of the input transistor by means of a feedback path, and an amplifier input connected to a second connection node between the current source and the second terminal of the input transistor. A level-shifting structure has a level-shifting element with one end connected to a reference connection, wherein the level-shifting element is adapted to perform a level-shifting of a potential at the second connection node with respect to a potential at the reference connection. The reference connection is coupled to one of the following: the amplifier output, the first connection node, a control terminal of the input transistor.

COMPOUNDS COMPRISING ONE OR MORE HYDROPHOBIC DOMAINS AND A HYDROPHILIC DOMAIN COMPRISING PEG MOIETIES, USEFUL FOR BINDING CELLS

The present invention relates to novel compounds comprising one or more hydrophobic domains and a hydrophilic domain comprising PEG moieties, useful for binding cells, as well as uses and compositions related thereto. The compounds are useful for immobilizing and/or stabilizing cells. 1. A compound comprising one or more hydrophobic domains and a hydrophilic domain ,wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, andwherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and {'br': None, 'sub': n', 'k1', 'n', 'k2, 'X1-[A1-(L1)]-Z-[A2-(L1)]-X2 \u2003\u2003(I),'}, 'wherein the hydrophilic domain comprises a compound of Formula (I)wherein{'sub': 2', '2, 'Z is linear polyethylene glycol (PEG) moiety containing 1 to 100 —O—CH—CH— moieties, wherein the polyethylene glycol moiety optionally comprises 1 or more spacer moieties SP connecting two'}{'sub': 2', '2, '—O—CH—CH— moieties, and wherein the linear PEG moiety optionally comprises a linker moiety L3 at one or both ends,'}each L1 is a linker moiety selected independently from each other,each n is either 0 or 1, selected independently from each other,A1 and A2 are bifunctional or trifunctional moieties selected independently from each other, with the proviso that at least one Al or A2 is trifunctional,k1 and k2 are integers between 0 and 10, selected independently from each other, with the proviso that at least one of k1 and k2 is not 0,X1 and X2 are independently selected from hydrogen or a protecting group,L3 is independently selected from a linear alkyl or alkenyl chain with 1 to 10 C atoms, which is optionally (i) interrupted by 1 to 3 N, O or S atoms, and/or (ii) substituted by 1 to 4 hydroxyl, carbonyl, amino or thiol groups,andwherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain via the trifunctional domain(s),or a salt thereof.2. The compound according to claim 1 , wherein Z ...

Method for ascertaining volume-or mass-flow of a medium

A method for ascertaining volume- or mass-flow of a medium in a pipeline, or measuring tube, of radius (r) via a sound entrainment method, wherein ultrasonic measuring signals are transmitted and/or received along a plurality of defined sound paths (m). During a calibration phase, successive, different flow profiles (v(r)) of a measured medium are established in the pipeline, or in the measuring tube, and wherein, for each flow profile (v(r)), a defined volume flow, or a defined mass flow, is ascertained, wherein, on the basis of measured values (v x (r 1 ) . . . v x (r m )) ascertained in the separate measuring paths (m), each flow profile (v(r)) established in the pipeline, or in the measuring tube, is described by a characteristic parameter set (a 1 , . . . , a n with nεN) and wherein, for each flow profile (V(r)), on the basis of the defined volume- or mass-flow and on the basis of the measured volume- or mass-flow, a flow-profile-dependent correction factor MF(a 1 , . . . , a n ) is calculated, and wherein, subsequently in measurement operation, a volume- or mass-flow ascertained via the parameter set (a 1 , . . . , a n , with nεN) is corrected with the flow-profile-dependent correction factor MF(a 1 , . . . , a n ) determined during the calibration phase.

Methods of specifically releasing a sub-group of objects

The present invention relates to a method of specifically releasing a one or more members of a sub-group of objects from an entity, a method of detecting a subject's disease by detecting one or more members of a sub-group of biological entities indicative of the disease and a method of isolating one or more members of a sub-group of objects from a group of objects.

Modular conveying unit

A conveying unit for a superordinate conveying system for transporting goods on a transport plane. The conveying unit has transport rollers, drive devices, at least one adjustment apparatus and a control unit. The transport rollers rotate about axes at an actuatable rotational speed and with an actuatable direction of rotation. The drive device can be actuated to drive the transport rollers. The axes of rotation of the transport rollers can be actuated about their respective pivot axes by their own pivot axis angle and can be adjusted by rotation. The adjustment apparatus can be actuated to adjust the axes of rotation of the transport rollers about their respective pivot axes. The control unit actuates the conveying unit, and the conveying unit additionally has a connection apparatus for connecting the conveying unit to the superordinate conveying system.

Microphone amplifier

A microphone amplifier comprises an input terminal (E100) for applying an input signal (IN), an output terminal (A100a) for outputting an output signal (OUT), and an additional output terminal (A100b) for outputting an additional output signal (VBIAS). The microphone amplifier also contains an amplifying circuit (10) for generating the output signal (OUT) by amplifying the input signal (IN), wherein the amplifying circuit (10) is connected between the input terminal (E100) and the output terminal (A100a), and a voltage generator (30) for generating the additional output signal (VBIAS). A supply voltage terminal (V30) of the voltage generator (30) is connected to the output terminal (A100a) of the microphone amplifier. Since the amplifying circuit (10) makes available a supply voltage (V) for the voltage generator (30), the microphone amplifier can be operated without a separate supply voltage terminal.

Conveyor unit having adjustable transport rollers

A conveying unit for transporting goods on transport rollers in a transport plane. The transport rollers can be rotated about their respective axes of rotation with an adjustable rotational speed and adjustable direction of rotation. The axes of rotation of the transport rollers are adjustable by rotation about their respective pivot axes by a particular pivot axis angle, and at least two of the transport rollers can be adjusted differently from one another. There is also described a method for adjusting the conveyor unit.

INTEGRATED INSTRUMENT PERFORMING SYNTHESIS AND AMPLIFICATION, AND A SYSTEM AND METHOD THEREOF

An integrated instrument for oligonucleotide synthesis and PCR, and a system and method thereof are disclosed. The integrated instrument is basically composed of two independent modules. The first module is a unit for chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample to be analyzed into the integrated instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.

SENSOR ARRANGEMENT

A sensor arrangement comprises a sensor having a first terminal and a second terminal, and an amplifier having an amplifier input for applying an input signal and an amplifier output for providing an amplified input signal, the amplifier input being coupled to the second terminal. The sensor arrangement further comprises a quantizer having a quantizer input and a quantizer output being suitable for providing a multi-level output signal on the basis of the amplified input signal and a feedback circuit having a feedback circuit input coupled to the quantizer output and a feedback circuit output coupled to the first terminal. The feedback circuit comprises a digital-to-analog converter being suitable for generating an analog signal on the basis of the multi-level output signal, the analog signal being the basis of a feedback signal provided at the feedback circuit output. The feedback circuit further comprises a feedback capacitor that is coupled between the feedback circuit output and an output of the digital-to-analog converter, and a voltage source coupled to the feedback circuit output.

Printed circuit board

The invention relates to a printed circuit board having an insulating support layer and a layer of conductive material applied thereto. In order to increase the flexibility of the printed circuit board, it is proposed that material from the support layer have been removed at at least one point on the printed circuit board on a side of the support layer which is opposite the layer of conductive material in order to form a straight groove which extends from one side of the printed circuit board to another side of the printed circuit board, so that the printed circuit board can be bent along the groove, wherein the material of the support layer which has remained in the region of the groove and/or the layer of conductive material in the region of the groove form(s) a bending edge.

Device for positioning a clamp-on flowmeter on a container

An apparatus for the positioning of a clamp-on flow measuring device on a containment, which has a medium flowing through it. The apparatus includes at least two ultrasonic transducers, which emit measuring signals into the containment and/or receive measuring signals from the containment, a positioning unit for the variable positioning of the ultrasonic transducers on the containment, a control/evaluation unit, which calculates from predetermined process and/or system variables a characteristic, desired variable of the measuring signals and which determines on the basis of a comparison of the calculated, desired variable with the corresponding, measured, actual variable, whether the ultrasonic transducers are optimally positioned or whether the position of the ultrasonic transducers need to be changed, and an indicating unit, which indicates to operating personnel a required change of position and/or the direction in which a change of position needs to be made.

USE OF A REAGENT FOR THE LYSIS OF ERYTHROCYTES AS WELL AS METHODS AND KITS RELATING THERETO

The present disclosure relates to use of a reagent for the lysis of erythrocytes, a method of lysing erythrocytes and a kit comprising the reagent. 1. A reagent for the lysis of erythrocytes , the reagent being an aqueous solution comprising HEPES ((4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) , NH/NH , a chelating agent and optionally CO/CO , wherein the final concentration during lysis of erythrocytes is in the range offrom 2.5 mmol/l to 12 mmol/l HEPES,{'sub': 4', '3, 'sup': '+', 'from 60 mmol/l to 120 mmol/l NH/NH,'}from 0.04 mmol/l to 0.8 mmol/l chelating agent, and{'sub': 3', '3, 'sup': 2−', '−, 'from 0.15 mmol/l to 0.8 mmol/l CO/CO, if present.'}2. The reagent of claim 1 , wherein the final concentration during lysis is in the range offrom 3 mmol/l to 11 mmol/l HEPES, preferably from 3 mmol/l to 10 mmol/l HEPES, more preferably from 3.5 mmol/l to 4.5 mmol/l HEPES,{'sub': 4', '3', '4', '3, 'sup': +', '+, 'from 70 mmol/l to 100 mmol/l NH/NH, preferably from 80 mmol/l to 85 mmol/l NH/NH,'}from 0.05 mmol/l to 0.5 mmol/l chelating agent, preferably from 0.06 mmol/l to 0.2 mmol/l chelating agent, more preferably from 0.07 mmol/l to 0.1 mmol/l chelating agent, and/or{'sub': 3', '3', '3', '3', '3', '3, 'sup': 2−', '−', '2−', '−', '2−', '−, 'from 0.3 mmol/l to 0.6 mmol/l CO/CO, preferably from 0.3 mmol/l to 0.5 mmol/1 CO/CO, more preferably from 0.35 mmol/l to 0.45 mmol/l CO/CO, if present.'}3. The reagent of claim 1 , wherein the chelating agent is ethylene diamine tetraacetic acid (EDTA).4. The reagent of claim 1 , wherein the pH of the reagent is in the range of from 6.4 to 7.7 claim 1 , preferably 6.7 to 7.4 claim 1 , more preferably 6.8 to 7.3.5. The reagent of claim 4 , wherein the pH is maintained in the range of from 6.4 to 7.7 claim 4 , preferably 6.7 to 7.4 claim 4 , more preferably 6.8 to 7.3 during lysis of erythrocytes.6. A method of lysing erythrocytes claim 4 , the method comprisinga) providing a sample comprising erythrocytes;{'claim-ref': [{'@ ...

ASSEMBLY FOR DETECTING ELECTROMAGNETIC RADIATION AND METHOD OF PRODUCING AN ASSEMBLY FOR DETECTING ELECTROMAGNETIC RADIATION

The assembly comprises a semiconductor device with an active-pixel array, a readout circuit chip or plurality of readout circuit chips mounted outside the active-pixel array, the readout circuit chip or plurality of readout circuit chips being configured to read out voltages or currents provided by the active-pixel array, and electric connections between the active-pixel array and the readout circuit chip or plurality of readout circuit chips.

Flowmeter

... flowmeter comprising at least two ultrasonic transducers that are mounted on a container which is penetrated by a medium in a certain direction of flow. The ultrasonic transducers alternately transmit and receive ultrasonic test signals in the direction of flow and counter to the direction of flow. The flowmeter further comprises a control/evaluation unit which determines and/or monitors the volume flow of the medium inside the container based on the difference in the travel time of the ultrasonic test signals propagating in the direction of flow and counter to the direction of flow. The ultrasonic transducers are configured such that they transmit and receive ultrasonic test signals or sound fields having a large aperture angle or a great beam expansion.

Use of compounds comprising two or more hydrophobic domains and a hydrophilic domain comprising PEG moieties for stabilization of a cell

The present invention relates to the use compounds comprising two or more hydrophobic domains and a hydrophilic domain comprising a polyethylene glycol (PEG) moiety for stabilization of a cell, and methods related thereto.

ADJUSTABLE HYDRAULIC PUMP

A hydraulic pump includes an adjustment device configured to adjust a delivery volume. The adjustment device is configured to be connected via a shuttle valve to a control pressure supply and to a working line. The working line is supplied by the hydraulic pump. The adjustment device is configured to be alternately supplied with control pressure by the control pressure supply and by the hydraulic pump. The hydraulic pump also includes a nozzle. The nozzle is configured to connect the working line to the control pressure supply in a zero stroke operating mode. The nozzle is formed in or on a valve body of the shuttle valve. 1. An adjustable hydraulic pump comprising:a shuttle valve having a valve body;an adjustment device configured to be connected via the shuttle valve to a control pressure supply and to a working line, the working line connected to the hydraulic pump; anda nozzle configured to continuously connect the working line to the control pressure supply, the nozzle in or on the valve body of the shuttle valve.2. The adjustable hydraulic pump according to claim 1 , wherein the nozzle is formed by a drilled hole.3. The adjustable hydraulic pump according to claim 1 , wherein:the valve body has a longitudinal drilled hole, andthe nozzle is formed by a radial drilled hole arranged in a central section of the valve body and which opens into the longitudinal drilled hole.4. The adjustable hydraulic pump according to claim 1 , wherein:the valve body has a first conical section and a first guide collar adjacent to the first conical section, andthe valve body has a second conical section and a second guide collar adjacent to the second conical section.5. The adjustable hydraulic pump according to claim 4 , wherein:the shuttle valve has an output connection;the first guide collar has at least one radial flattened portion, via which, in a first switched position of the valve body, a first pressure medium connection from the control pressure supply to the output ...

MEMS microphone assembly and method for fabricating a MEMS microphone assembly

A micro-electro-mechanical system, MEMS, microphone assembly comprises an enclosure defining a first cavity, and a MEMS microphone arranged inside the first cavity. The microphone comprises a first die with bonding structures and a MEMS diaphragm, and a second die having an application specific integrated circuit, ASIC. The second die is bonded to the bonding structures such that a gap is formed between a first side of the diaphragm and the second die, with the gap defining a second cavity. The first side of the diaphragm is interfacing with the second cavity and a second side of the diaphragm is interfacing with the environment via an acoustic inlet port of the enclosure. The bonding structures are arranged such that pressure ventilation openings are formed that connect the first cavity and the second cavity.

Sensors with corrugated diaphragms

A sensor includes a substrate; and a corrugated diaphragm offset from the substrate. The corrugated diaphragm is configured to deflect responsive to a sound wave impinging on the corrugated diaphragm. A cavity is defined between the corrugated diaphragm and the substrate, the corrugated diaphragm forming a top surface of the cavity and the substrate forming a bottom surface of the cavity. A pressure in the cavity is lower than a pressure outside of the cavity.

MEMS sensor

A MEMS sensor (1) comprises a MEMS transducer (10) being coupled to a MEMS interface circuit (20). The MEMS interface circuit (20) comprises a bias voltage generator (100), a differential amplifier (200), a capacitor (300) and a feedback control circuit (400). The bias voltage generator (100) generates a bias voltage (Vbias) for operating the MEMS transducer. The variable capacitor (300) is connected to one of the input nodes (I200a) of the differential amplifier (200). At least one of the output nodes (A200a, A200b) of the differential amplifier is coupled to a base terminal (T110) of an output filter (110) of the bias voltage generator (100). Any disturbing signal from the bias voltage generator (100) is a common-mode signal that is divided equally on the input nodes (I200a, I200b) of the differential amplifier (200) and is therefore rejected.

METHOD OF IMMOBILIZING A CELL ON A SUPPORT USING COMPOUNDS COMPRISING A POLYETHYLENE GLYCOL MOIETY

The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support. 1. A method of immobilizing a cell on a support , the method comprising wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and', 'wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and', 'wherein the compound comprises a linking group;, 'a) providing a compound or salt thereof comprising one or more hydrophobic domains attached to a hydrophilic domain,'}b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; andc) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.2. The method of claim 1 , wherein the compound comprises one or more hydrophobic domains and a hydrophilic domain claim 1 ,wherein the one or more hydrophobic domains are ...

Device providing coordinated emission of light and volatile active

A flameless candle that releases a volatile active includes at least one LED positioned in a tip (106), a cartridge mount (128), and a support structure (102, 141). The at least one LED emits a flickering light that emulates a flame of a candle. The cartridge mount (128) receives and secures a replaceable cartridge (104a) containing a volatile active to be released into the atmosphere over time. The support structure (120, 141) supports the at least one LED and the cartridge mount (128). The support structure (120, 141) is configured to allow airflow across the replaceable cartridge (104a) when the replaceable cartridge (104a) is mounted in the cartridge mount (128).

Hydraulic Flushing Valve Arrangement

A hydraulic flushing valve arrangement includes a flushing valve having a housing, flushing piston, and at least one spring. The housing includes a valve bore, two circular housing control edges, two inlet channels fluidically connected to the bore, and an outlet channel opening into the bore between the inlet channels. The piston includes two control collars, a piston neck, and piston control edges that delimit the collars from the neck, and that have circular shapes with a recess. The recess has a portion that, in a cross-sectional plane perpendicular to an axis of the piston, is constant and sized such that a flushing quantity is reduced when the portion of the recess is situated in a region of a corresponding housing control edge relative to a quantity when the piston is in an end position. The collars guide the piston so as to be longitudinally displaceable in the bore, and are spaced apart by the neck. The piston and control edges are configured to control the fluidic connections between the bore, the inlet channels, and the outlet channel. The spring preloads the piston in a middle position whereat the outlet channel is blocked from the inlet channels. The piston is configured to move out of the middle position in response to a specific difference between pressures of the two inlet channels. The control collars are respectively configured to open the fluidic connection between a corresponding inlet channel in which a lower pressure prevails and the outlet channel. Such a flushing valve arrangement is usable in hydrostatic travel drives in which two hydromachines are operated in a closed hydraulic circuit. 1. A hydraulic flushing valve arrangement , comprising: [ a valve bore;', 'two circular housing control edges;', 'two inlet channels flowing into the valve bore; and', 'an outlet channel opening into the valve bore between the two inlet channels;, 'a housing, having, a piston neck;', 'two piston control edges, each piston control edge having a circular shape ...

Split signal differential MEMS microphone

An integrated circuit includes a first amplifier and a second amplifier. A first impedance matching circuit is coupled to the first amplifier, a first charge pump, and a single MEMS transducer. A second impedance matching circuit is coupled to the second amplifier, a second charge pump, and to the single MEMS transducer. A first capacitive load as measured at an input of first amplifier, and a second capacitive load as measured at an input of the second amplifier exist. The first capacitive load and the second capacitive load are balanced with respect to each other. A single pressure change causes the single MEMS transducer to create a first electrical signal and a second electrical signal. Both the first electrical signal and the second electrical signal are matched or approximately matched in magnitude, and 180 degrees or approximately 180 degrees out of phase with respect to each other.

Device for determining and/or monitoring the volume, and/or mass, flow rate of a medium

An ultrasonic flow measuring device which provides a flow measuring device characterized by a low electrical current consumption and low power uptake. At least one component of high power uptake with a control/evaluation unit is provided, with the control/evaluation unit being designed such that the component of high power uptake is operated intermittently in a measuring phase and in an idle phase, with the component being activated in the measuring phase, while, in the idle phase, the component exhibits a reduced power uptake or is completely turned off.

Sensor arrangement

A sensor arrangement includes a sensor having a first terminal and a second terminal, and an amplifier having an amplifier input for applying an input signal and an amplifier output for providing an amplified input signal, the amplifier input being coupled to the second terminal. A quantizer having a quantizer input and a quantizer output is configured to provide a multi-level output signal on the basis of the amplified input signal and a feedback circuit having a feedback circuit input coupled to the quantizer output and a feedback circuit output coupled to the first terminal. The feedback circuit includes a digital-to-analog converter configured to generate an analog signal on the basis of the multi-level output signal, the analog signal being the basis of a feedback signal provided at the feedback circuit output, a feedback capacitor coupled between the feedback circuit output and an output of the digital-to-analog converter, and a voltage source coupled to the feedback circuit output.

Adjustable hydraulic pump

A hydraulic pump includes an adjustment device configured to adjust a delivery volume. The adjustment device is configured to be connected via a shuttle valve to a control pressure supply and to a working line. The working line is supplied by the hydraulic pump. The adjustment device is configured to be alternately supplied with control pressure by the control pressure supply and by the hydraulic pump. The hydraulic pump also includes a nozzle. The nozzle is configured to connect the working line to the control pressure supply in a zero stroke operating mode. The nozzle is formed in or on a valve body of the shuttle valve.

Lighting Device for a Motor Vehicle

The invention relates to a lighting device for a motor vehicle. Said lighting device comprises a light source (1; 96) for emitting electromagnetic radiation, in particular light which is visible to the human eye, and a reflector (70; 81; 90) for focusing the emitted light. The light source (1; 96) is fastened on the reflector (70; 81; 90) at least indirectly in a defined position relative to a reflective surface of said reflector. In order firstly to make heat transfer from the reflector (70; 81; 90) to the light source (1; 96) more difficult and secondly to enable EMC shielding by virtue of an electrical contact between the light source (1; 96) and the reflector (70; 81; 90), the invention proposes that a material (72) with poor thermal conductivity is arranged between the reflector (70; 81; 90) and the light source (1; 96) for thermally insulating the light source (1; 96) from the reflector (70; 81; 90). Preferably, the material (72) has a thermal conductivity of less than 2 W/mK and ...

Labeling reagent

The current invention restates substituted nitroindole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted nitroindole nucleosides can be used as labeling reagents for the facile preparation of, e.g., optimized hybridization probes, simple probes, TAQMAN-probes or molecular beacon probes.

Method for ascertaining volume -or mass- flow of a medium

A method for ascertaining volume- or mass-flow of a medium in a pipeline, or measuring tube, of radius (r) via a sound entrainment method, wherein ultrasonic measuring signals are transmitted and/or received along a plurality of defined sound paths (m). During a calibration phase, successive, different flow profiles (v(r)) of a measured medium are established in the pipeline, or in the measuring tube, and wherein, for each flow profile (v(r)), a defined volume flow, or a defined mass flow, is ascertained, wherein, on the basis of measured values (v)x(r1) . . . vx(rm)) ascertained in the separate measuring paths (m), each flow profile (v(r)) established in the pipeline, or in the measuring tube, is described by a characteristic parameter set (a1 . . . an with nεN) and wherein, for each flow profile (V(r)), on the basis of the defined volume- or mass-flow and on the basis of the measured volume- or mass-flow, a flow-profile-dependent correction factor MF(a1, . . . , an) is calculated, and ...

SPLIT SIGNAL DIFFERENTIAL MEMS MICROPHONE

An integrated circuit includes a first amplifier and a second amplifier. A first impedance matching circuit is coupled to the first amplifier, a first charge pump, and a single MEMS transducer. A second impedance matching circuit is coupled to the second amplifier, a second charge pump, and to the single MEMS transducer. A first capacitive load as measured at an input of first amplifier, and a second capacitive load as measured at an input of the second amplifier exist. The first capacitive load and the second capacitive load are balanced with respect to each other. A single pressure change causes the single MEMS transducer to create a first electrical signal and a second electrical signal. Both the first electrical signal and the second electrical signal are matched or approximately matched in magnitude, and 180 degrees or approximately 180 degrees out of phase with respect to each other. 1. An integrated circuit , comprising:a first amplifier;a second amplifier;a first impedance matching network coupled to the first amplifier, a first charge pump, and a single MEMS transducer;a second impedance matching network coupled to the second amplifier, a second charge pump, and the single MEMS transducer;a first capacitive load; anda second capacitive load, wherein the first capacitive load and the second capacitive load are sized to equalize total capacitance including parasitic capacitances of an interconnect and the single MEMS transducer; andwherein a single pressure change causes the single MEMS transducer to create a first electrical signal and a second electrical signal, each of the first electrical signal and the second electrical signal being matched or approximately matched in magnitude, and 180 degrees or approximately 180 degrees out of phase with respect to each other.2. The integrated circuit of claim 1 , wherein an electrical bias is applied across the single MEMS transducer by the first charge pump and the second charge pump.3. The integrated circuit of ...

Electric amplifier circuit for amplifying an output signal of a microphone

An electric amplifier circuit for amplifying an output signal of a microphone comprises a supply input terminal (V10) to apply a supply potential (VDDA) for operating the electric amplifier circuit and a differential amplifier (110) having a first input terminal (E110a) for applying the output signal of the microphone (20), a second input terminal (E110b) and an output terminal (A110) for outputting an amplified output signal (OUT) of the microphone (20). A feedback path (FP) is provided between the output terminal (A110) of the differential amplifier (110) and the second input terminal (E110b) of the differential amplifier (110). A charge supplying circuit (120) is coupled to the feedback path (FP) to supply an amount of the charge to the feedback path (FP) in dependence on the supply potential (VDDA). The amount of charge supplied to the feedback path may be dependent on a change of the supply potential (VDDA).

MINIATURIZED, HIGH-THROUGHPUT NUCLEIC ACID ANALYSIS

The present invention is directed to method for analyzing multiple nucleic acid molecules of interest comprising in the steps of (i) providing a plurality of beads, characterized in that each bead comprises at least two sequence specific amplification primers, further characterized in that at least one of the primers is bound to the bead via a cleavable linker, (ii) capturing the nucleic acid molecules of interest from a sample, (iii) clonally isolating the plurality of beads, (iv) cleaving the at least one primer, (v) clonally amplifying the nucleic acid thereby creating multiple amplification products, and (vi) analyzing the amplification products.

SOLID SUPPORT FOR HIGH-THROUGHPUT NUCLEIC ACID ANALYSIS

The present invention provides a solid support which is preferably a bead comprising at least two sequence specific amplification primers wherein at least one primer is bound to the support with an inducible cleavable linker. The present invention also provides various method for preparing a solid support comprising at least two sequence specific primers, further characterized in that at least one of the primers is cleavable.

Low-pass filter arrangement

In an embodiment a low-pass filter arrangement has an input terminal for receiving an input voltage, a first voltage source coupled to the input terminal, a serial connection comprising a first and a second filter diode, the serial connection being coupled to the first voltage source, wherein a connection point between the first and the second filter diode is coupled to an output terminal of the filter arrangement, and a first filter capacitor coupled between the output terminal and a filter reference potential terminal. Therein the first voltage source is adapted to provide a first adjustable forward voltage whereby the first and the second filter diodes are both biased in a forward direction.

Lighting device for a motor vehicle

The invention relates to a lighting device for a motor vehicle. Said lighting device comprises a light source (1; 96) for emitting electromagnetic radiation, in particular light which is visible to the human eye, and a reflector (70; 81; 90) for focusing the emitted light. The light source (1; 96) is fastened on the reflector (70; 81; 90) at least indirectly in a defined position relative to a reflective surface of said reflector. In order firstly to make heat transfer from the reflector (70; 81; 90) to the light source (1; 96) more difficult and secondly to enable EMC shielding by virtue of an electrical contact between the light source (1; 96) and the reflector (70; 81; 90), the invention proposes that a material (72) with poor thermal conductivity is arranged between the reflector (70; 81; 90) and the light source (1; 96) for thermally insulating the light source (1; 96) from the reflector (70; 81; 90). Preferably, the material (72) has a thermal conductivity of less than 2 W/mK and ...

Apparatus for determining and/or monitoring volume and/or mass flow of a medium

An ultrasonic flow measuring device, which determines volume and/or mass flow of a medium in a pipeline, or in a measuring tube. The flow measuring device includes a plurality of ultrasonic sensors, which emit and/or receive ultrasonic measurement signals along defined sound paths. The control/evaluation electronics of the flow measuring device is divided into an on-site electronics, in which at least one switch is provided, via which the ultrasonic sensors, arranged in different sound paths, can be driven and/or queried, and at least one remote, control/evaluation unit, wherein the control/evaluation unit so switches the at least one switch, that the driven and/or queried, ultrasonic sensor, or the driven and/or queried pair of ultrasonic sensors, is activated and provides measured values.

Flowmeter mounted on a containment

Flowmeter comprising at least two ultrasonic transducers that are mounted on a container which is penetrated by a medium in a certain direction of flow. The ultrasonic transducers alternately transmit and receive ultrasonic test signals in the direction of flow and counter to the direction of flow. The flowmeter further comprises a control/evaluation unit which determines and/or monitors the volume flow of the medium inside the container based on the difference in the travel time of the ultrasonic test signals propagating in the direction of flow and counter to the direction of flow. The ultrasonic transducers are configured such that they transmit and receive ultrasonic test signals or sound fields having a large aperture angle or a great beam expansion.

Microphone Amplifier

A microphone amplifier comprises an input terminal (E 100 ) for applying an input signal (IN), an output terminal (A 100 a ) for outputting an output signal (OUT), and an additional output terminal (A 100 b ) for outputting an additional output signal (VBIAS). The microphone amplifier also contains an amplifying circuit ( 10 ) for generating the output signal (OUT) by amplifying the input signal (IN), wherein the amplifying circuit ( 10 ) is connected between the input terminal (E 100 ) and the output terminal (A 100 a ), and a voltage generator ( 30 ) for generating the additional output signal (VBIAS). A supply voltage terminal (V 30 ) of the voltage generator ( 30 ) is connected to the output terminal (A 100 a ) of the microphone amplifier. Since the amplifying circuit ( 10 ) makes available a supply voltage (V) for the voltage generator ( 30 ), the microphone amplifier can be operated without a separate supply voltage terminal.

MODULAR CONVEYING UNIT

A conveying unit for a superordinate conveying system for transporting goods on a transport plane. The conveying unit has transport rollers, drive devices, at least one adjustment apparatus and a control unit. The transport rollers rotate about axes at an actuatable rotational speed and with an actuatable direction of rotation. The drive device can be actuated to drive the transport rollers. The axes of rotation of the transport rollers can be actuated about their respective pivot axes by their own pivot axis angle and can be adjusted by rotation. The adjustment apparatus can be actuated to adjust the axes of rotation of the transport rollers about their respective pivot axes. The control unit actuates the conveying unit, and the conveying unit additionally has a connection apparatus for connecting the conveying unit to the superordinate conveying system. 115-. (canceled)16. A conveying unit for a superordinate conveying system for transporting goods on a transport plane , the conveying unit comprising:a plurality of transport rollers mounted for rotation about respective axes of rotation at an actuatable rotational speed and with an actuatable direction of rotation;drive devices configured for driving said transport rollers;the axes of rotation of said transport rollers being pivotable about respective pivot axes by a pivot axis angle;an adjustment apparatus configured for adjusting an orientation of the axes of rotation of said transport rollers by rotation about respective pivot axes;a control unit configured for actuating said adjustment apparatus and said drive devices; anda connection apparatus for connecting the conveying unit to the superordinate conveying system.171. The conveying unit according to claim , wherein at least two of said transport rollers are able to be actuated differently from one another.18. The conveying unit according to claim 16 , wherein said drive devices are configured for individual actuation so as to individually drive a respective ...

Balance having a load changing device and method for operating said balance

A balance with a load changing device ( 14 ) that has a magazine table, mounted to be horizontally movable and having a plurality of vertical openings ( 26 ) arranged in groups. The balance ( 12 ) has a load cell ( 34 ) and a load receptor ( 42 ) with a carrier arrangement ( 44 ) corresponding to each of the groups of openings. The magazine table and the balance are height adjustable relative to each other. In a weighing position, a group of openings above the load receptor is penetrated by the carrier arrangement. In a changing position, the carrier arrangement is positioned lower than the openings in the magazine table. The magazine table is mounted at a fixed height relative to a base ( 16 ), and the balance is height adjustable relative to the base. The travel of the balance is upwardly limited by a stop ( 32 ), which is at a fixed height relative to the base.

MEMS microphone assembly and method for fabricating a MEMS microphone assembly

A micro-electro-mechanical system, MEMS, microphone assembly comprises an enclosure defining a first cavity, and a MEMS microphone arranged inside the first cavity. The microphone comprises a first die with bonding structures and a MEMS diaphragm, and a second die having an application specific integrated circuit, ASIC. The second die is bonded to the bonding structures such that a gap is formed between a first side of the diaphragm and the second die, with the gap defining a second cavity. The first side of the diaphragm is interfacing with the second cavity and a second side of the diaphragm is interfacing with the environment via an acoustic inlet port of the enclosure. The bonding structures are arranged such that pressure ventilation openings are formed that connect the first cavity and the second cavity. 2. The MEMS microphone assembly according to claim 1 , wherein the gap height is larger than 10 μm.3. The MEMS microphone assembly according to claim 1 , wherein the pressure ventilation openings are defined byvoids between clamping structures of the diaphragm and the bonding structures in a main extension plane of the diaphragm; orvoids of the bonding structures.4. The MEMS microphone assembly according to claim 1 , wherein the second die comprises an opening that connects the first cavity and the second cavity.5. The MEMS microphone assembly according to claim 1 , wherein at least one dimension of the pressure ventilation openings corresponds to the gap height.6. The MEMS microphone assembly according to claim 1 , wherein the MEMS microphone consists of the first die and the second die.7. The MEMS microphone assembly according to claim 1 , further comprising an optical readout assembly having at least a light source and a detector claim 1 , wherein the optical readout assembly is configured to detect a displacement of a point or a surface of the diaphragm claim 1 , in particular a point or a surface of the first side of the diaphragm.8. The MEMS microphone ...

Conveyor Unit Having Adjustable Transport Rollers

A conveying unit for transporting goods on transport rollers in a transport plane. The transport rollers can be rotated about their respective axes of rotation with an adjustable rotational speed and adjustable direction of rotation. The axes of rotation of the transport rollers are adjustable by rotation about their respective pivot axes by a particular pivot axis angle, and at least two of the transport rollers can be adjusted differently from one another. There is also described a method for adjusting the conveyor unit. 115-. (canceled)16. A conveying unit for transporting goods in a transport plane , the conveying unit comprising:the transport rollers rotatably mounted about respective axes of rotation with an adjustable rotational speed and an adjustable direction of rotation; andthe axes of rotation of said transport rollers being adjustable by rotation about respective pivot axes by a given pivot axis angle;wherein at least two of said transport rollers are adjustable differently from one another.17. The conveying unit according to claim 16 , wherein the axes of rotation are substantially parallel to the transport plane and the pivot axes are substantially orthogonal to the transport plane.18. The conveying unit according to claim 16 , wherein said transport rollers have a rounded surface in an area intended for contact with the goods.19. The conveying unit according to claim 16 , further comprising an adjustment device configured to jointly adjust the pivot axis angle of two or more of said transport rollers.20. The conveying unit according to claim 16 , further comprising actuatable drive devices for driving a transport roller.21. The conveying unit according to claim 20 , wherein said drive devices are servo drives.22. The conveying unit according to claim 16 , wherein said transport rollers are selectively drivable in a positive direction of rotation and a negative direction of rotation opposite the positive direction.23. The conveying unit according to ...

Delta-sigma modulator and method for signal conversion

A delta-sigma modulator ( 10 ) comprises a modulator loop ( 11 ) and a code generator ( 12 ). The modulator loop ( 11 ) comprises a loop filter ( 18 ). The code generator ( 12 ) is configured to generate a generator signal (BS) that is realized as an extended Barker code. The code generator ( 12 ) comprises a generator output ( 23 ) that is coupled to the loop filter ( 18 ).

Signal processing arrangement, sensor arrangement and signal processing method

A signal processing arrangement has a signal input for connecting a capacitive sensor. An amplifier circuit is coupled between the signal input and a feedback point. A loop filter is coupled downstream to the feedback point. A quantizer is connected downstream to the loop filter and provides a multi-bit output word. The multi-bit output word consists of one or more higher significance bits and one or more lower significance bits. A first feedback path is coupled between a quantizer and the feedback point for providing a first feedback signal to the feedback point being representative of the one or more lower significance bits. A second feedback path is coupled to the quantizer for providing a second feedback signal to the signal input being representative of the one or more higher significance bits.

AMPLIFIER ARRANGEMENT AND AMPLIFICATION METHOD

An amplifier arrangement has an input transistor being connected between reference potential terminals by a current source and a current sink. An amplifier stage has an amplifier output coupled to a first connection node between the current sink and a first terminal of the input transistor by means of a feedback path, and an amplifier input connected to a second connection node between the current source and the second terminal of the input transistor. A level-shifting structure has a level-shifting element with one end connected to a reference connection, wherein the level-shifting element is adapted to perform a level-shifting of a potential at the second connection node with respect to a potential at the reference connection. The reference connection is coupled to one of the following: the amplifier output, the first connection node, a control terminal of the input transistor. 1. An amplifier arrangement comprisingan input transistor with a first terminal coupled to a first reference potential terminal via a current sink, with a second terminal coupled to a second reference potential terminal via a current source, and with a control terminal connected to a signal input; andan amplifier stage with an amplifier output coupled to a first connection node between the current sink and the first terminal of the input transistor via a feedback path, and with an amplifier input connected to a second connection node between the current source and the second terminal of the input transistor;a level-shifting structure comprising a level-shifting element with one end connected to a reference connection that is coupled to one of the following: the amplifier output, the first connection node, the control terminal of the input transistor;wherein the level-shifting element is adapted to perform a level-shifting of a potential at the second connection node with respect to a potential at the reference connection.2. The amplifier arrangement according to claim 1 ,wherein the level- ...

Electric amplifier circuit for amplifying an output signal of a microphone

An electric amplifier circuit for amplifying an output signal of a microphone comprises a supply input terminal (V 10 ) to apply a supply potential (VDDA) for operating the electric amplifier circuit and a differential amplifier ( 110 ) having a first input terminal (E 110 a ) for applying the output signal of the microphone ( 20 ), a second input terminal (E 110 b ) and an output terminal (A 110 ) for outputting an amplified output signal (OUT) of the microphone ( 20 ). A feedback path (FP) is provided between the output terminal (A 110 ) of the differential amplifier ( 110 ) and the second input terminal (E 110 b ) of the differential amplifier ( 110 ). A charge supplying circuit ( 120 ) is coupled to the feedback path (FP) to supply an amount of the charge to the feedback path (FP) in dependence on the supply potential (VDDA). The amount of charge supplied to the feedback path may be dependent on a change of the supply potential (VDDA).

BEND-FORMED LOAD-BEARING STRUCTURE OF A PASSENGER CAR

The invention relates to a method, use or a profile for manufacturing metallic flat materials to a cost-effective load-bearing structure of a passenger car and the usage of it. As the main forming manufacturing method the bend forming according to DIN EN ISO 8580 is used. 1. Method for manufacturing a load-bearing structure of a passenger carcharacterized in that the bend forming according to DIN EN ISO 8586 is usedas the main forming procedure for the structure (≥50%) manufactured out of metallic flat materials whereby load-bearing structure is defined as a land-based automotive vehicle or attached or pulled structure of it and whereby bend forming is carried out as a multi-edged forming process for most of the metallic flat materials (≥50%).2. Method for manufacturing a load-bearing structure of a passenger car according to the claim 1 , characterized in that the load-bearing structure that is used is metallic flat material and have a thickness ≤6.0 mm claim 1 , more preferably ≤3.0 mm.3. Method for manufacturing a load-bearing structure of a passenger car according to claim 1 , characterized in that the as-delivered condition of the used metallic flat material before a necessary preparatory cutting step and the following bend forming step is a coil claim 1 , a strip claim 1 , a plate claim 1 , a sheet claim 1 , a blank claim 1 , a contoured or shaped sheet claim 1 , or a panel.4. Method for manufacturing a load-bearing structure of a passenger car according to claim 1 , characterized in that the load-bearing structure is used with a blank metallic surface without an additional painting or cathodic dip coating.5. Method for manufacturing a load-bearing structure of a passenger car according to claim 1 , characterized in that the metallic flat material is preferably a stainless steel claim 1 , more preferably a cold-hardened stainless steel.6. Method for manufacturing a load-bearing structure of a passenger car according to claim 1 , characterized in that the ...

Shared safety cell for passenger cars

The present invention relates to a passive safety cell (2) for passenger cars with alternative powertrains which is shared for the passengers (3) as well as for the energy source (4) with its main components, to protect both units in one cell.

3-Substituted 5-Nitroindole derivatives and labeled oligonucleotide probes containing them

Nitroindole derivatives and labeled oligonucleotide probes containing them

The current invention restates substituted nitroindole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted nitroindole nucleosides can be used as labeling reagents for the facile preparation of e.g. optimized hybridization probes, simple probes, Taqmanprobes or molecular beacon probes.

Beads for high-throughput nucleic acid analysis

The present invention provides a solid support which is preferably a bead comprising at least two sequence specific amplification primers wherein at least one primer is bound to said support with an inducible cleavable linker. The present invention also provides various method for preparing a solid support comprising at least two sequence specific primers, further characterized in that at least one of said primers is cleavable .

Microfluidic chip, device and system for the generation of aqueous droplets in emulsion oil for nucleic acid amplification

Pulse generator

The invention relates to a pulse generator P consisting of a control logic SL which controls a switch SR by means of control pulses, said switch being connected to a voltage supply unit S on the input side and supplying a pulse voltage on the output side. A capacitor C01 is connected between the control logic SL and the switch S, said capacitor reducing the pulse voltage if the pulse frequencies of the control pulse are too small. An RC element is arranged between the voltage supply unit S and the switch SR, said RC element reducing the pulse voltages if the repetition rates of the control pulses are too high. The invention thus enables the pulse generator to be used in hazardous areas.

Use of compounds comprising two or more hydrophobic domains and a hydrophilic domain comprising peg moieties for stabilization of a cell

The present invention relates to the use compounds comprising two or more hydrophobic domains and a hydrophilic domain comprising a polyethylene glycol (PEG) moiety for stabilization of a cell, and methods related thereto.

Compounds comprising one or more hydrophobic domains and a hydrophilic domain comprising PEG residues, useful for joining cells

Un compuesto que comprende, preferentemente que consiste en, uno o más dominios hidrófobos y un dominio hidrófilo, en el que el uno o más dominios hidrófobos se unen covalentemente a dicho dominio hidrófilo, y en el que el uno o más dominios hidrófobos comprenden cada uno un lípido lineal, que es un ácido graso saturado que tiene de 12 a 18 átomos de C, el esteroide colesterol o la vitamina hidrófoba α-tocoferol, y en el que el dominio hidrófilo comprende un compuesto de Fórmula (I): X1-[A1-(L1)n]k1-Z-[A2-(L1)n]k2-X2 (I), en la que Z es un resto de polietilenglicol (PEG) lineal que contiene de 1 a 50, preferentemente 4-30 restos -O-CH2-CH2-, en el que el resto de polietilenglicol comprende opcionalmente 1 o más restos espaciadores SP que conectan dos restos -O-CH2-CH2-, y en el que el resto de PEG lineal comprende opcionalmente un resto enlazador L3 en uno o ambos extremos, cada L1 es un resto enlazador seleccionado independientemente entre sí, cada n es 0 o 1, seleccionado independientemente entre sí, A1 y A2 son restos bifuncionales o trifuncionales seleccionados independientemente entre sí, a condición de que al menos uno de A1 o A2 sea trifuncional, el resto trifuncional se selecciona entre lisina, serina, serinol, -O-CH2- CH((CH2)4-NH2)-CH2-, un glicerol y un resto 1,3-diaminoglicerol, en la que el resto bifuncional se selecciona entre el grupo que consiste en un grupo fosfato, grupo carbamato, grupo amida, un resto que comprende una nucleobase, incluso más preferentemente dT, y un grupo alquilo lineal que tiene de 1 a 10 átomos de C, y cuya cadena de alquilo contiene grupos funcionales en los átomos de C terminales, en particular independientemente seleccionados entre grupos amino, carbonilo, hidroxilo, tiol, ácido carbónico, k1 y k2 se seleccionan independientemente entre sí, y k1 es 1, 2, 3, 4 o 5, y k2 es 1, 2, 3, 4, 5 o 6, X1 y X2 se seleccionan independientemente entre hidrógeno o un grupo protector, L3 se selecciona independientemente entre una cadena ...

Method of immobilizing a cell on a support using compounds comprising a polyethylene glycol moiety

The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

Method for determining the volume flow rate or mass flow rate of a medium

The invention relates to a method for determining the volume flow rate or mass flow rate of a medium (3) in a pipe (3) or a test tube having a radius (r) via a harmonic excitation process. According to said method, ultrasonic test signals are transmitted and/or received along several defined sound paths (m). Different successive flow profiles (v(r)) of a measured medium (2) are embodied in the pipe (3) or test tube during a calibration phase, a defined volume flow rate or a defined mass flow rate is determined for each flow profile (v(r)), each flow profile (v(r)) embodied in the pipe (3) or test tube is described by a characteristic parameter set (a1,, an, wherein n ∈ N) based on the measured values (v(r1),, v(rm)) determined in the individual measuring paths (m), a flow profile-dependent corrective factor MF(a1,, an) is calculated for each flow profile (V(r)) based on the defined volume flow rate or mass flow rate and the measured volume flow rate or mass flow rate, whereupon a volume flow rate or mass flow rate determined via the parameter set (a1,, an, wherein n ∈ N) is corrected in the measuring mode with the flow profile-dependent corrective factor MF(a1,, an) determined during the calibration phase.

Nitroindole derivatives and labeled oligonucleotide probes containing them

The current invention restates substituted nitroindole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted nitroindole nucleosides can be used as labeling reagents for the facile preparation of e.g. optimized hybridization probes, simple probes, Taqmanprobes or molecular beacon probes.

Motor-drive unit e.g. for adjustment device mounted in motor vehicle door panel

The invention relates to an engine-gearbox unit for adjustment devices in motor vehicles, comprising a base plate (2) which supports the engine-gearbox unit and which can be mounted in an opening (10) of a wall (1) separating a wet area (A) from a dry area (B). The gearbox unit output (5) is located on the wet area end (A) and at least the electrical elements for electrical contacting of the electric motor (6) and optionally a switch and/or an electronic control device (7) are located on the dry area end (B). A sealing area (3) encompassing the opening (10) is located between the wall (1) and the base plate (2). The base plate (2) forms a single unit with at least with one section of the gearbox housing (20) and is fitted onto the wall (1) from the wet area end (A).

Active material emitting device

Use of a reagent for the lysis of erythrocytes, as well as procedures and kits related thereto

Uso de un reactivo para la lisis de eritrocitos, siendo el reactivo una solución acuosa que comprende o que consiste en HEPES (ácido (4-(2-hidroxietil)-1-piperacinetanosulfónico), NH4+/NH3, un agente quelante, y opcionalmente CO32-/HCO3- en el que la concentración final durante la lisis de eritrocitos está en el intervalo de - desde 2,5 mmol/l a 12 mmol/l de HEPES, - desde 60 mmol/l a 120 mmol/l de NH4+/NH3, - desde 0,04 mmol/l a 0,8 mmol/l de agente quelante, y - desde 0,15 mmol/l a 0,8 mmol/l de CO32-/HCO3-, si está presente, en el que el agente quelante se selecciona del grupo que consiste en EGTA (ácido etilenglicol-tetraacético), BAPTA (ácido 1,2-bis(o-aminofenoxi)etano-N,N,N',N'-tetraacético) DTPA (ácido dietilentriaminopentaacético), EDTA (tetraacetato de etilendiamina) y NTA (N,N-bis(carboximetil)glicina). Use of a reagent for the lysis of erythrocytes, the reagent being an aqueous solution comprising or consisting of HEPES ((4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), NH4+/NH3, a chelating agent, and optionally CO32 -/HCO3- where the final concentration during erythrocyte lysis is in the range of - from 2.5 mmol/l to 12 mmol/l HEPES, - from 60 mmol/l to 120 mmol/l NH4+/NH3, - from 0.04 mmol/l to 0.8 mmol/l of chelating agent, and - from 0.15 mmol/l to 0.8 mmol/l of CO32-/HCO3-, if present, in which the chelating agent is selected from the group consisting of EGTA (ethylene glycol tetraacetic acid), BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid), DTPA (diethylenetriaminepentaacetic acid), EDTA ( ethylenediamine tetraacetate) and NTA (N,N-bis(carboxymethyl)glycine).

LIGHTING DEVICE OF A MOTOR VEHICLE

L'invention concerne un dispositif d'éclairage de véhicule automobile, comportant une lampe à décharge (1 ; 96) pour émettre de la lumière, un dispositif d'amorçage pour générer une tension d'amorçage pour allumer la lampe à décharge (1; 96), et un dispositif de commande pour générer une tension d'entrée pour le dispositif d'amorçage et une tension de service pour le fonctionnement de la lampe à décharge (1 ; 96), le dispositif de commande étant une partie intégrante du dispositif d'amorçage. Pour résoudre en même temps la problématique de place et de température, il est prévu dans le dispositif d'amorçage et de commande combiné (5 ; 80) un élément de connexion (6), pour le raccordement à une tension du réseau de bord (7), l'élément (6) étant réalisé sous forme de carte de circuits imprimés (20) avec des pistes d'interconnexion (21 ; 23) guidées sur un bord d'une carte de circuits imprimés (20) du dispositif d'amorçage et de commande (5 ; 80). The invention relates to a lighting device for a motor vehicle, comprising a discharge lamp (1; 96) for emitting light, an ignition device for generating an ignition voltage for igniting the discharge lamp (1; 96), and a control device for generating an input voltage for the starting device and an operating voltage for the operation of the discharge lamp (1; 96), the control device being an integral part of the device priming. To solve the problem of space and temperature at the same time, a connection element (6) is provided in the combined ignition and control device (5; 80), for connection to a voltage from the on-board network ( 7), the element (6) being in the form of a printed circuit board (20) with interconnecting tracks (21; 23) guided on one edge of a printed circuit board (20) of the device. priming and control (5; 80).

Miniaturized, high-throughput nucleic acid analysis

The present invention is directed to method for analyzing multiple nucleic acid molecules of interest comprising in the steps of (i) providing a plurality of beads, characterized in that each bead comprises at least two sequence specific amplification primers, further characterized in that at least one of said primers is bound to the bead via a cleavable linker, (ii) capturing the nucleic acid molecules of interest from a sample, (iii) clonally isolating said plurality of beads, (iv) cleaving said at least one primer, (v) clonally amplifying said nucleic acid thereby creating multiple amplification products, and (vi) analyzing said amplification products.

Active material emitting device

An active material cartridge comprises a frame and an active material refill. The active material refill comprises at least one reservoir having an active material therein and a protrusion extending from a first end thereof. The active material refill is disposed on and attached to the frame.

Clonal pre-amplification in emulsion

The present invention is directed to a process for clonal pre-amplification of a nucleic acid comprising the steps of (i) providing a plurality of different nucleic acid molecules (b) attaching adaptor sequences to the 3' ends and 5' ends of said nucleic acid molecules (c) preparing a water in oil emulsion characterized in that the majority of water droplets comprises one or none member of said plurality of different nucleic acid molecules (d) clonally amplifying said plurality of different nucleic acid molecules. In particular, said different nucleic acid molecules are mRNA molecules.

Method of immobilizing a cell on a support using compounds comprising a polyethylene glycol moiety

The present invention relates to a method of immobilizing a cell on a support, the method comprising a) providing a compound or salt thereof comprising, preferably consisting of, one or more hydrophobic domains attached to a hydrophilic domain, wherein the one or more hydrophobic domains are covalently bound to said hydrophilic domain, and wherein the one or more hydrophobic domains each comprise a linear lipid, a steroid or a hydrophobic vitamin, and wherein the hydrophilic domain comprises a polyethylene glycol (PEG) moiety, and wherein the compound comprises a linking group; b) contacting a cell with the compound under conditions allowing the interaction of the compound with the membrane of the cell, thereby immobilizing the linking group on the surface of the cell; and c) contacting the linking group immobilized on the cell with a support capable of binding the linking group, thereby immobilizing the cell on the support.

Headlights for vehicles, in particular by means of a gas discharge lamp.

Encrusted element for use in clock element and in jewelry article, has recess that form decoration imprint, and rail anchor and metal decoration allowed to communicate with recess to provide anchoring of metal decoration against element

L’invention se rapporte à un élément (10) comportant un corps (11) comprenant au moins un évidement formant l’empreinte d’un décor, ledit au moins un évidement étant entièrement rempli par un dépôt (16) galvanique afin de former un élément (10) incrusté d’au moins un décor métallique à la qualité visuelle améliorée. Selon l’invention, l’élément (10) comporte un dispositif d’ancrage dudit au moins un décor métallique communiquant avec ledit au moins un évidement afin d’améliorer l’ancrage dudit au moins un décor contre ledit élément. L’invention concerne le domaine des pièces d’horlogerie, de bijouterie on de joaillerie, et le domaine des arts de la table.

Use of compounds comprising two or more hydrophobic domains and a hydrophilic domain comprising peg moieties for stabilization of a cell

The present invention relates to the use compounds comprising two or more hydrophobic domains and a hydrophilic domain comprising a polyethylene glycol (PEG) moiety for stabilization of a cell, and methods related thereto.

Miniaturized, high-throughput nucleic acid analysis

The present invention is directed to method for analyzing multiple nucleic acid molecules of interest comprising in the steps of (i) providing a plurality of beads, characterized in that each bead comprises at least two sequence specific amplification primers, further characterized in that at least one of said primers is bound to the bead via a cleavable linker, (ii) capturing the nucleic acid molecules of interest from a sample, (iii) clonally isolating said plurality of beads, (iv) cleaving said at least one primer, (v) clonally amplifying said nucleic acid thereby creating multiple amplification products, and (vi) analyzing said amplification products.

Conveyor unit comprising adjustable transport rollers

Die Erfindung offenbart eine Fördereinheit (2) zum Transport von Gütern (4) auf einer Transportebene (6) umfassend Transportrollen (8). Die Transportrollen (8) sind mit einstellbarer Drehzahl und einstellbarer Drehrichtung um ihre jeweiligen Drehachsen (10) rotierbar. Die Drehachsen (10) der Transportrollen (8) sind um ihre jeweiligen Schwenkachsen (12) um einen eigenen Schwenkachsenwinkel (14) durch Rotation verstellbar und mindestens zwei der Transportrollen (8) sind voneinander abweichend einstellbar. Die Erfindung offenbart zudem ein Verfahren zum Einstellen der Fördereinheit (2). The invention discloses a conveyor unit (2) for the transport of goods (4) on a transport plane (6) comprising transport rollers (8). The transport rollers (8) are rotatable about their respective axes of rotation (10) with adjustable speed and adjustable direction of rotation. The axes of rotation (10) of the transport rollers (8) about their respective pivot axes (12) about its own pivot axis angle (14) by rotation adjustable and at least two of the transport rollers (8) are different from each other adjustable. The invention also discloses a method for adjusting the conveyor unit (2).

Integrated instrument performing synthesis and amplification

The present invention is directed to an integrated system for oligonucleotide synthesis and PCR. The integrated instrument of the inventive system is basically composed of two independent modules which are operatively linked to each other. The first module is a unit for the chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample that shall be analyzed into the inventive instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.

Device for positioning a clamp-on flowmeter on a container

Die Erfindung bezieht sich auf eine Vorrichtung zur Postionierung eines Clamp-On Durchflussmessgeräts (1) an einem Behältnis (7), das von einem Medium (9) durchströmt wird, mit zumindest zwei Ultraschallwandlern (2; 3), die Messsignale in das Behältnis (7) aussenden und/oder aus dem Behältnis (7) empfangen, mit einer Positioniereinheit (11) zur variablen Positionierung der Ultraschallwandler (2, 3) an dem Behältnis (7), mit einer Regel-/Auswerte-einheit (8), die aus vorgegebenen Prozess- und/oder Systemgrössen eine charakteristische SOLL-Grösse (Tsoll) der Messsignale errechnet und die anhand eines Vergleichs der errechneten SOLL-Grösse (Tsoll) mit der entsprechenden gemessenen IST-Grösse (Tist) ermittelt, ob die Ultraschallwandler (2, 3) optimal positioniert sind oder ob die Position der Ultraschallwandler (2, 3) zu verändern ist, und mit einer Anzeigeeinheit (12), die dem Bedienpersonal eine erforderliche Positionsänderung und/oder die Richtung in der eine Positionsänderung zu erfolgen hat, zur Anzeige bringt.

Analysis of single nucleotide polymorphisms using mass spectrometry

A method for mass spectrometric analysis of polymorphisms or mutations in genomic DNA, by selective replication of short DNA strands by PCR covering the polymorphism, which methods comprises employing at least one derivatised nucleoside triphosphate in the PCR amplification, wherein derivatisation is such that the mass of the nucleoside is altered. and detection by mass spectrometry is enhanced. Preferably the mass of the nucleoside triphosphate derivative is increased by at least 14 atomic mass units, and more preferably is a derivative of a 7-desaza-purine or 8-aza-7-desaza-purine nucleoside. Kits for carrying out the invention are also disclosed.

Method and device for monitoring the interior of a vehicle

Es wird ein Verfahren (100) zur Überwachung (112) eines Innenraums eines Fahrzeuges vorgeschlagen, das eine Aufnahme (110) von Bildern mittels einer Kamera (11) umfasst, wobei die Kamera (11) hinter einem Spiegelglas (21) eines beweglichen Rückblickspiegels (20) des Fahrzeuges angeordnet ist und wobei das Verfahren (100) ein Erkennen (101) einer Position des beweglichen Rückblickspiegels (20) des Fahrzeuges umfasst. A method (100) for monitoring (112) an interior of a vehicle is proposed, which comprises recording (110) images by means of a camera (11), the camera (11) being located behind a mirror glass (21) of a movable rear-view mirror ( 20) of the vehicle and wherein the method (100) comprises detecting (101) a position of the movable rearview mirror (20) of the vehicle.

Axialkolbenmaschine mit einem führungsschenkel für ein käfigsegment

Signal processing arrangement, sensor arrangement and signal processing method

A signal processing arrangement (100) has a signal input (SI) for connecting a capacitive sensor (CS). An amplifier circuit (AMP) is coupled between the signal input (SI) and a feedback point (CB1). A loop filter (LF) is coupled downstream to the feedback point (CB1). A quantizer (QT) is connected downstream to the loop filter (LF) and provides a multi-bit output word (OW). The multi-bit output word (OW) consists of one or more higher significance bits (HSB) and one or more lower significance bits (LSB). A first feedback path (FB1) is coupled between a quantizer (QT) and the feedback point (CB1) for providing a first feedback signal to the feedback point (CB1) being representative of the one or more lower significance bits (LSB). A second feedback path is coupled to the quantizer (QT) for providing a second feedback signal to the signal input (SI) being representative of the one or more higher significance bits (HSB).

Method and device for monitoring an electric energy store

In a method for monitoring an electric energy store, comprising at least one electric energy storage unit, an electronic battery management unit and an interface for communication with external devices, the method comprises the steps: a) determining at least one state variable of the electric energy store, particularly a voltage quantity and/or a state of charge quantity, by the electronic battery management unit; b) transmitting the determined state variable via the interface for communication with external devices to a monitoring device (40, 50) being installed externally to the electric energy store; c) evaluating a state of the electric energy store by the monitoring device (40, 50) depending on the determined at least one state variable; d) performing a charging process of the electric energy store by a charging device that is situated externally to the electric energy store depending on the evaluation by the monitoring device (40, 50) and/or performing a voltage balancing process inside of the electric energy store by an internally installed voltage balancing device. The monitoring device (40, 50) comprises a communication device (41, 51) and evaluation device (42, 52).

Methods of specifically releasing a sub-group of objects

The present invention relates to a method of specifically releasing a one or more members of a sub-group of objects from an entity, a method of detecting a subject's disease by detecting one or more members of a sub-group of biological entities indicative of the disease and a method of isolating one or more members of a sub-group of objects from a group of objects.

Vorrichtung zur positionierung eines clamp-on durchflussmessgeräts an einem behältnis

Current to digital converter circuit, optical front end circuit, computed tomography apparatus and method

A current to digital converter circuit has an integrator amplifier (IAmp) with an input (12) adapted to receive a current signal (Ip) and an output (13) adapted to provide a voltage signal (Vout) as a function of an integration of the current signal (Ip), a quantizer circuit (Op2) with an input which is coupled to the output (13) of the integrator amplifier (IAmp) and with an output (14) adapted to provide a binary result signal (Scmp1) as a function of a comparison of the voltage signal (Vout) with at least a first reference voltage signal (Vref1), a digital-to-analog converter circuit (Dac) which is coupled in a switchable manner as a function of the binary result signal (Scmp1) to the input (12) of the integrator amplifier (IAmp), and a controlled current source (CCS) which is coupled to the output (13) of the integrator amplifier (IAmp) via a first switch (S1) which is controlled as a function of the binary result signal (Scmp1) such that an auxiliary current (Iaux) is supplied to the output (13) of the integrator amplifier (IAmp).

Vorrichtung und Verfahren zum Transport eines Wägegutes auf den Lastaufnehmer einer Waage.

Eine Vorrichtung zum Transport eines Wägegutes auf den Lastaufnehmer (2) einer Waage mit einem beweglichen Wägegutträger (4) weist einen zweiten, fest stehenden oder beweglichen Wägegutträger (10) mit einer Zentrierhilfe (11) und mindestens zwei Kraftmesszellen (12) oder Momentenaufnehmer zur Bestimmung der Lage des Schwerpunktes des Wägegutes auf dem zweiten Wägegutträger (10) auf. Die Vorrichtung weist ausserdem Rechen- und Steuermittel auf, die aus der Abweichung der Lage des Schwerpunktes des Wägegutes auf dem zweiten Wägegutträger (10) von seiner Solllage die Verfahrbewegung des ersten (4) und gegebenenfalls auch des zweiten Wägegutträgers (10) so errechnet und steuert, dass das Wägegut die exakte Solllage auf dem Lastaufnehmer (2) erreicht. Dadurch lässt sich unabhängig von der Geschicklichkeit des Bedieners eine exakte Lage des Wägegutes auf dem Lastaufnehmer der Waage erreichen, wodurch Ecklastfehler bei oberschaligen Waagen und Pendelbewegungen des Lastaufnehmers bei unterschaligen Waagen vermieden werden.

Ultraschallmassendurchflussmesser

Apparat til regulering af tykkelsen af en overtraeksmasse

Einrichtung zur regulierung des bodenwalles an ueberziehanlagen

Die Einrichtung zur Regulierung des Bodenwalles ist Teil einer Ueberziehanlage. Auf Ueberziehanlagen dieser Art werden Dauerbackwaren, Marzipan u. ae. allseitig mit Ueberzugmasse, wie z. B. fluessige Schokolade oder Kuvertuere, beschichtet. Das Ziel der Erfindung besteht darin, eine Einrichtung zu entwickeln, durch die eine unkomplizierte Bedienung mit wenig Bedienaufwand realisierbar ist. Der Erfindung liegt die Aufgabe zugrunde, eine Einrichtung zur Regulierung des Bodenwalles zu entwickeln, die ueber ein Bedienelement verstellbar ist. Erfindungsgemaess wird die Aufgabe dadurch geloest, dass eine Einrichtung zur Regulierung des Bodenwalles entwickelt wurde, die aus einer Foerderwalze besteht, ueber die ein Gitterband gefuehrt wird, und mindestens zwei Funktionselemente aufweist, die derart angeordnet sind, dass die Funktionselemente fest mit einem schwenkbar gelagerten Gestell verbunden sind und dem Gestell ein Hebel zugeordnet ist, wobei die Funktionselemente hinsichtlich ihrer Lage zum Gestell einstellbar sind.

Verfahren und vorrichtung zur herstellung von rahmen

Schaltung zur Stabilisierung der Spannung an einer Lampe, insbesondere für Scheinwerferlampen eines Kraftfahrzeuges

１８カラット３ｎ金合金およびこれの析出方法

【課題】主成分として亜鉛もカドミウムも含まない、厚い、３Ｎの黄色に着色した１８カラットの金合金層を析出させるための析出方法を提供すること。 【解決手段】本発明は、金金属、有機金属化合物、湿潤剤、金属イオン封鎖剤および遊離シアン化物を含む浴に浸漬された電極上での金合金のガルバノプラスチック析出方法であって、合金金属は銅金属および銀金属であり、鏡のように輝く黄色の金合金は電極上に析出され、この浴は、２１．５３％の金、７８．３１％の銅および０．１６％の銀の比率とすることを特徴とする方法に関する。本発明は、電気析出の分野に関する。 【選択図】なし

Ultraschallmassendurchflussmesser

Verfahren und Vorrichtung zur Herstellung von Rahmen

Delta-sigma modulator and method for signal conversion

A delta-sigma modulator (10) comprises a modulator loop (11) and a code generator (12). The modulator loop (11) comprises a loop filter (18). The code generator (12) is configured to generate a generator signal (BS) that is realized as an extended Barker code. The code generator (12) comprises a generator output (23) that is coupled to the loop filter (18).

レーザー保護材料およびレーザー保護構成材

【課題】有効波長帯を有するレーザー保護フィルターに使用するレーザー保護材料を提供する。 【解決手段】レーザー保護材料は、有効波長帯において透過性であるプラスチックのマトリックス材料（２）及びマトリックス材料（２）に実質的に均一な形態で埋め込まれている吸収材料（３）から成り、吸収材料（３）は有効波長帯においてレーザー照射（４）を吸収し、閾値温度を超える温度に加熱されたとき有効波長帯においてその吸収能力を消失するものであり、マトリックス材料（２）のプラスチックは閾値温度を超える温度に加熱されたとき発砲し始めるものである。 【選択図】図１

Apparat til regulering af tykkelsen af en overtraeksmasse

Sensors with corrugated diaphragms

A sensor includes a substrate; and a corrugated diaphragm offset from the substrate. The corrugated diaphragm is configured to deflect responsive to a sound wave impinging on the corrugated diaphragm. A cavity is defined between the corrugated diaphragm and the substrate, the corrugated diaphragm forming a top surface of the cavity and the substrate forming a bottom surface of the cavity. A pressure in the cavity is lower than a pressure outside of the cavity.

Polyester plasticizers for vinyl chloride resins

Polyester plasticizers for vinyl chloride resins

Suszeptometer.

Control and an integrated circuit for a multisensory apparatus

A control for a multisensory apparatus comprises means for detecting a number of lights connected to the control. The control further includes means responsive to the detecting means for operating the light(s) connected to the control in either of first and second different modes of operation in dependence upon the detected number of lights connected thereto. Still further, the control includes means for actuating an active material dispenser.

Suszeptometer.