Method for detecting potato virus and viroid by once composite PCR reaction

The invention discloses a detecting technique of potato virus and viroid molecule and atopic primer, which is characterized by the following: detecting four potato viruses (PVX, PVY, PLRV and PVS)and one potato viroid (PSTV)through once compound PCR; improving the atopy and detecting efficiency with low cost.

Medical image recognizing method

The invention relates to a medical image recognition method, which aims at providing the method which can more accurately recognize the type of a new medical image. The method comprises the construction of a classification library of association rules, the update thereof and the medical image recognition step, the construction of the classification library of the association rules and the update step thereof comprise the following steps: data of medical image samples are prepared and carried out the pre-treatment; a density clustering-based medical image segmentation method is adopted to respectively recognize local region to be analyzed in each sample medical image; the characteristics of the local region in each sample medical image are respectively extracted to construct a medical image sample database T, the characteristics comprise: mean, variance, inclination, kurtosis, energy, entropy and clustering characteristics; the characteristic values are carried out the discretization; the ...

Method of efficient production of test tube potato and culture box thereof

Polynucleotide sequence for regulating low-temperature sweetening of potatoes and application thereof

The invention discloses a polynucleotide sequence regulating low-temperature sweetening of potatoes and an application of the polynucleotide sequence. The polynucleotide sequence is shown in SEQ ID NO:1 in a sequence table. According to the invention, after the polynucleotide sequence is obtained through two genes StBAM1 and StBAM9, the two genes StBAM1 and StBAM9 are simultaneously intervened in potato, so that the content of reducing sugar can be very remarkably reduced and the low-temperature sweetening resistance of potato can be improved. The function of the polynucleotide sequence is far higher than the respective functions of StAmy23, StBAM1 and StBAM9 in low-temperature sweetening.

Image defogging method with optimal contrast ratio and minimal information loss

The invention relates to an image defogging method with optimal contrast ratio and minimal information loss. The image defogging method is characterized in that through the adoption of functions including the Gauss mixed model, the quadtree, the maximal contrast ratio and the minimal information loss, the image defogging method is realized. The image defogging method comprises the following steps: firstly, based on the Gauss mixed model and the expected value maximal algorithm, images are segmented into two categories, namely, a sky region and a non-sky region; secondly, the quadtree iteration method is adopted in the sky region of the images to estimate the atmospheric illumination intensity of an atmospheric scattering model; then, the grid partition method is adopted to partition the non-sky region of the images and the functions of the maximal contrast ratio and the minimal information loss are adopted to calculate the spreading rate of an atmospheric lighting model of each grid unit ...

Online self-adaptive intertissue implantation method and equipment based on 3D printing guide plate

The invention provides an online self-adaptive intertissue implantation method and equipment based on a 3D printing guide plate. The method comprises the following steps: S1, carrying out CT scanning on an object with a standard mold to obtain object three-dimensional image information and mold information; s2, importing the CT image into a three-dimensional planning system, sketching a mold and a target region PTV and an organ-at-risk OAR on the CT image, and reconstructing standard mold information; s3, a physicist designs a planting plan and plan optimization through an automatic plan according to the position relation between the mold and the target region hook in a plan design system; s4, generating an individualized needle passage guide part printing file based on the plan information, and performing rapid printing; s5, the guide plate component and a standard mold are assembled and matched, and pin insertion is carried out according to plan parameters; s6, carrying out CT scanning ...

Application of cisplatin combined with hTERT-HRP/IAA in medicament for treating cervical cancer cell

The invention discloses an application of cisplatin combined with phTERTp-HRP/IAA in drugs for the treatment of cervical cancer cells, in particular to the strengthening role of the cisplatin on the activity of a hTERT gene promoter. The cisplatin and the phTERTp-HRP/IAA are commonly used in the synergy for killing the human cervical cancer cells, and the action mechanism thereof is further explored. The application adopts Hela cells for carrying out the study and evaluates the impacts of the cisplatin on the activity of the hTERT promoter through the expression of downstream luciferase reporter gene regulated by the hTERT promoter. The synergic killing role and the mechanism of the cisplatin and the hTERT promoter during the combined use for regulating the gene therapy of an HRP/IAA system are evaluated through the detection of cell proliferation rate, cell apoptosis rate and cell cycle. The results confirm that the cisplatin can strengthen the activity of the hTERT gene promoter; and ...

Radiation sensibility tumor-targeted promotor and uses thereof

The invention discloses a radiation-sensitive tumor target promoter and application thereof, relates to construction of a chimeric promoter, potentiation of the radiation to activity of the chimeric promoter and synergic action of combination of radiation and gene treatment in killing tumor cells. Normal cells and three different tumor cells are adopted for research, and specificity of the chimeric promoter and the effect of radiation on the activity of the chimeric promoter can be evaluated through expression of a downstream luciferase reporter gene regulated by the chimeric promoter. Synergic killing action when the radiation and the HRP/IAA regulated by the chimeric promoter are combined can be evaluated through inspecting cell proliferation rate and cell apoptosis rate. Results prove that the chimeric promoter has tumor specificity, radiation can enhance the activity of the chimeric promoter; when the radiation is combined with the chimeric promoter, the chimeric promoter has obvious ...

Medical image recognizing method

The invention relates to a medical image recognition method, which aims at providing the method which can more accurately recognize the type of a new medical image. The method comprises the construction of a classification library of association rules, the update thereof and the medical image recognition step, the construction of the classification library of the association rules and the update step thereof comprise the following steps: data of medical image samples are prepared and carried out the pre-treatment; a density clustering-based medical image segmentation method is adopted to respectively recognize local region to be analyzed in each sample medical image; the characteristics of the local region in each sample medical image are respectively extracted to construct a medical image sample database T, the characteristics comprise: mean, variance, inclination, kurtosis, energy, entropy and clustering characteristics; the characteristic values are carried out the discretization; a ...

Potato T-DNA elements and homologous transgenic system and application thereof

The invention discloses 25 potato T-DNA elements and a homologous transgenic system and an application thereof. Through a lot of test researches and exploration, the 25 potato T-DNA elements are finally obtained, and nucleotide sequences of the 25 potato T-DNA elements are shown in a sequence 1 to a sequence 25 in a sequence table. Genetic transformation shows that the 25 potato T-DNA elements and potato homologous transgenic carriers formed by the elements can be used in potato safe transgene genetics and breeding, and provide homologous transgenic system without mark or frame for the potato plant.

Radiation sensibility tumor-targeted promotor and uses thereof

The invention discloses a radiation-sensitive tumor target promoter and application thereof, relates to construction of a chimeric promoter, potentiation of the radiation to activity of the chimeric promoter and synergic action of combination of radiation and gene treatment in killing tumor cells. Normal cells and three different tumor cells are adopted for research, and specificity of the chimeric promoter and the effect of radiation on the activity of the chimeric promoter can be evaluated through expression of a downstream luciferase reporter gene regulated by the chimeric promoter. Synergic killing action when the radiation and the HRP/IAA regulated by the chimeric promoter are combined can be evaluated through inspecting cell proliferation rate and cell apoptosis rate. Results prove that the chimeric promoter has tumor specificity, radiation can enhance the activity of the chimeric promoter; when the radiation is combined with the chimeric promoter, the chimeric promoter has obvious ...

一种高效生产马铃薯试管薯的方法及其培养盒

The invention is a fast tube potato breeding method and the equipment. Use detoxicated potato non-virus tube seedling by tissue culture as material, adopt MS culture medium, without any grower to obtain the test-tube potato seedling with no any growth material and its cane sugar concentration 6%-10%, then place in culture box for liquid culture, light intensity 2000-3000lux, and illuminate for about 8 hours every day, and according to sensitivity of different potato varieties to short sunlight, make illumination adjustment. It designs a special culture box for tube potato, composed of under tube body and box cover, ventilate column designed on the cover. It can produce for 5-6 times each year. Unit area of culture room can produce 100,000-200,000 tube potatoes.

CD47 protein ubiquitination modification agonist and application thereof

The invention discloses a CD47 protein ubiquitination modification agonist and application thereof, and belongs to the technical field of CD47 ubiquitination promotion. The USP2 inhibitor ML364 is used for inhibiting the ubiquitination modification function of USP2, the ubiquitination modification of CD47 is promoted, the protein expression quantity of CD47 is reduced, the anti-tumor immune response of immune cells in tumor tissue is activated, and the anti-tumor immunotherapy effect is enhanced. The CD47 ubiquitination activator can enhance immune response in tumor tissues, has a synergistic effect when being combined with other immunodetection point antibodies, such as anti-PD-1 antibodies, and can enhance the effect of single drug use so as to better treat tumors. Compared with the existing CD47 blocking agent, the CD47 protein ubiquitination modification agonist provided by the invention has the advantages that the quantity of red blood cells and blood platelets cannot be influenced ...

Combined miniature potato seed counter

The invention relates to a combined miniature potato seed counter which comprises an operating handle, a through hole type counting plate combination, a hinge axis combination, a mobile baffle combination and a positioning device, wherein the operating handle is arranged at the front end of the through hole type counting plate combination; the positioning device is arranged on the lower layer of the operating handle; the through hole type counting plate combination and the mobile baffle combination are connected into a whole through the hinge axle combination; and 200 frustum regular conical counting holes are arranged on the through hole type counting plate combination so as to facilitate the entrance of miniature potato seeds, also facilitate the smooth discharge of the miniature potato seeds and be difficult to cause the mechanical damage to the miniature potato seeds. The combined miniature potato seed counter has the characteristics that the combined miniature potato seed counter has ...

Post-installation planting plan needle passage optimization method and device

The invention provides an after-installation planting plan needle passage optimization method and equipment. The method comprises the following steps: S1, constructing an object three-dimensional coordinate system based on a CT or MRI image, and reconstructing PTV and OAR three-dimensional space information; s2, setting the position and the range of an object surface pin surface in the object three-dimensional coordinate system, setting physical limiting conditions between pin channels according to the three-dimensional space position relation of PTV and OAR and in combination with the sizes of inserted pins, and calculating all pin paths; s3, defining a penalty function model, calculating a position relation between a prescription dose line and PTV and OAR in a three-dimensional space according to dose distribution and attenuation characteristics of the radioactive source, and generating the penalty function model; and S4, defining the number of pins, calculating the penalty function values ...

Micro potato seed precision sorter

The invention relates to a micro potato seed precision sorter. A shaker sorting device shakes forward and backward, leftwards and rightwards in operating process so that micro potato seeds can be respectively clearly sorted when passing through vertical trapezoid sieve pore regions in different specifications, sieve pore region damping plates matched with the vertical trapezoid sieve pore regions can effectively control skip-grade separation of the micro potato seeds and ensure the accuracy of micro potato seed sorting; and the inclination, the amplitude and reciprocating speed of forward and backward shaking, the amplitude and reciprocating speed of leftward and rightward and the arranging speed and quantity of a sorting and seed arranging box can be freely adjusted. The sorter has the characteristics of reasonable structure, accurate sorting, high working efficiency, low use cost and less mechanical damage to the micro potato seeds, is applicable to the sorting of irregular spherical ...

Uses of gamma-ray combined phTERT-HRP/IAA in medicament for treating cancer of larynx

The invention discloses application of the combination of gamma ray and phTERTp-HRP/IA in medicines for killing larynx cancer cells, and in particular relates to the enhancement of the gamma ray in promoter activity of hTERT gene and the synergetic effect of the combination of the gamma ray and the phTERTp-HRP/IA for killing the human larynx cancer cells. The invention studies levels of different radiation sensitivity cancer cells in cells and animals and detects the promoter activity and enzymatic activity, volume, weight and tumor inhibiting rate of transplanted tumor, tumor tissue HE coloration and apoptosis of lower-reach gene expression products to evaluate the synergetic killing effect when the gamma ray treatment and the hTERT promoter regulating-controlling HRP/IAA system gene treatment are jointly used. The result shows that when the gamma ray treatment and the phTERTp-HRP/IAA treatment are jointly used, the human larynx cancer cells are obviously killed.

Application of cisplatin combined with hTERT-HRP/IAA in medicament for treating cervical cancer

The invention discloses an application of cisplatin combined with phTERTp-HRP/IAA in drugs for the treatment of cervical cancer cells, in particular to the strengthening role of the cisplatin on the activity of a hTERT gene promoter. The cisplatin and the phTERTp-HRP/IAA are commonly used in the synergy for killing the human cervical cancer cells, and the action mechanism thereof is further explored. The application adopts Hela cells for carrying out the study and evaluates the impacts of the cisplatin on the activity of the hTERT promoter through the expression of downstream luciferase reporter gene regulated by the hTERT promoter. The synergic killing role and the mechanism of the cisplatin and the hTERT promoter during the combined use for regulating the gene therapy of an HRP/IAA system are evaluated through the detection of cell proliferation rate, cell apoptosis rate and cell cycle. The results confirm that the cisplatin can strengthen the activity of the hTERT gene promoter; and ...