Operon for synthesizing Pantoea agglomerans beta-carotene, expression vector and use thereof

The invention discloses a Beta-carotene synthesis operon of pantoea agglomerans. The Beta-carotene synthesis operon has the total length of 6241bp, is obtained by cultivation, total DNA extraction and PCR augmentation, and has a sequence of SEQ ID No 1. The sequence and an expression carrier of the Beta-carotene synthesis operon can be applied into the production of Beta-carotene.

Antifreeze transcription factor derived from common wheat AP2/ERF family and preparation method and application thereof

The invention discloses an antifreeze transcription factor derived from the common wheat AP2/ERF family and a preparation method and application thereof. The antifreeze transcription factor of the common wheat AP2/ERF family is a TaERF-B3 gene, wherein the base sequence of the antifreeze transcription factor is shown as SEQ ID No 1; and the amino acid sequence of the protein of the antifreeze transcription factor is shown as SEQ ID No 2. In the invention, the gene sequence of the common wheat AP2/ERF family transcription factor is cloned from the common wheat seedling by a polymerase amplification technology. The obtained transcription factor gene is used for plant transformation to improve plant stress resistance through cultivation.

Gene directed molecular evolution system in vitro based on half-rational evolutionary design

The invention relates to a simple and efficient gene in vitro directed evolution system based on half-reasoning design; the system can achieve larger mutation potential and more definite mutation region compared with the conventional reshuffle mutation by combining DNA reshuffle mutation and the half-reasoning design and can provide more diversified mutant population for the modification of gene and protein, wherein, the mutant sites of the diversified mutant population are positioned in critical area.. With the combination of a screening system, reliable improved gene or protein can be obtained more easily; moreover, the system of the invention has effectiveness as well as simple and convenient operation.

耐高温β-葡萄糖苷酸酶基因及其获得方法

... 一种耐高温GUS基因，是通过DNA突变过程获得GUS突变基因，再构建成表达载体，转化入大肠杆菌中，进行耐高温性筛选获得的耐85℃的耐高温GUS基因克隆，并对耐高温GUS基因进行了序列测定，结果表明该耐高温GUS基因具有1812个核苷酸。耐高温GUS基因可简便地应用于转基因植物转化体的筛选，极大地提高转基因植株的检测效率，促进植物转基因研究的发展。 ...

Automatic control system of garbage crane

The invention discloses an automatic control system for a garbage crane, which comprises a data acquisition unit used for acquiring working environment parameters and operation data of the garbage crane and state information of equipment, scanning a garbage library of the garbage crane through a preset scanner, acquiring various scanning coordinate information, and transmitting the scanning coordinate information to a control unit; an image video of the garbage crane in the working process is obtained through a camera device; the data analysis platform is used for calculating operation control parameters of the garbage crane according to the environment parameters and the operation data, and constructing a three-dimensional space model by scanning coordinate information; the control unit is used for controlling feeding, stacking, unloading, ditching and shaping of the garbage crane according to the data information on the basis of the operation rule and the operation area through the PLC ...

Gene order of coding 5-enolpyruvyl shikimate-3-phosphate synthase from apple

The invention relates to the field of plant genetic engineering, in particular to a gene of 5-enolpyruvyl shikimate-3-phosphate synthase. The method discloses a gene sequence which codes the 5-enolpyruvyl shikimate-3-phosphate synthase and is cloned from apple seedling through utilizing a rapid amplification technology on cDNA end.

Production for phytase with high living rate high temp. resisting by pichia

The invention discloses utilization of pichia pastoris to produce high-specific activity high temperature- resistant phytase, adopts many isogenous phytase genes, using DNA enzyme to make partial enzyme mediation, recovering all the DNA degraded fragments, in vitro rearranging DNA molecules, making enzyme cutting on all the rearranged DNA molecules, then composing them in an expression carrier, electrically shocking them in Escherichia coli, screening mutant phytase gene by phytase expression and activity, and finally obtaining high-specific activity high temperature-resistant phytase gene Phy XH, which is constructed into phytase gene expression carrier pPhy XH, electrically shocking and transferring in pichia pastoris, and selecting recombinant high-phytase expression pichia pastoris.

EPSP synthase gene descended from Pseudomonas fluorescens and application thereof

Cold resistant correlated transcription factor gene of rice and uses thereof

The invention belongs to the field of crop genetic breeding, and in particular relates to a gene related to rice cold tolerance and application thereof. The transcription factor gene related to the rice cold tolerance has a base sequence of SEQ ID NO.1. The transcription factor gene RCBF4 can be used for plant transformation so as to improve cold tolerance and hyperhaline resistance. The stress resistant gene originates from plant self, so the gene has smaller influence on environment.

Adopt ultrasonic atomization subassembly to reinforce blendor to fixed effect of flying dust heavy metal entrapment

The utility model relates to a having adopted the ultrasonic atomization subassembly, having strengthened the blendor of chelating agent solution fixed efficiency of entrapment of heavy metal dust particle in to waste incineration fly ash, included ribbon mixer promptly, it can be to spouting the ultrasonic atomization subassembly into atomized liquid in the ribbon mixer in succession still to have included. The utility model discloses a have the convection current, shed two ribbon mixer of immixture, supplementaryly uses granularity that the ultrasonic atomization effect produced as the slight liquid pearl of micron order, furthest has realized superfine powder of flying dust and chelating agent atomized liquid abundant, quick, even, efficient contact mixed process between the two, the material that the device belongs to the large scale solid -to -liquid ratio carries out high -efficient mixed technical equipment.

Antifreeze CBF transcription factor gene of brassica napus

The invention provides an antifreeze CBF transcription factor gene of brassica napus, a method for preparing the same and application thereof. The antifreeze CBF transcription factor gene of the brassica napus is BnaCBF-2-Hy15 with a base sequence represented by SEQ ID No 1. In the antifreeze CBF transcription factor gene of the brassica napus, the polymerase amplification technique is adopted to clone the gene sequence of the antifreeze CBF transcription factor of the brassica napus from seedlings of the rape and the obtained CBF transcription factor gene can be used for phytotransformation to develop and improve the antifreeze property of the plants.

用化学合成和分子进化获得酵母表达高比活植酸酶基因

A phytase gene phyI with high specific activity suitable for yeast expression is prepared from synthetic gene through configuring mutant phytase gene library on prokaryotic expresstion plasmid, transferring to colibacillus, screening high-activity strains, and high-density fermenting.

Phytase high throughput screen system based on pyroxylin membrance

The invention relates to a phytase high-throughput screening system based on a nitrocellulose membrane. In the system, a phytase mutant library obtained by directed molecular evolution in vitro or chemical mutation is coated on a solid culture medium, chromogenic screening is performed after the photolithography of the nitrocellulose membrane, and the method can obtain larger screening capacity than test tube culture, and can perform a large amount of screening of the phytase mutant libraries in a short time. The phytase mutation and the high-throughput screening are developed through the phytase high-throughput screening system based on the nitrocellulose membrane, which can greatly improve the mutation screening efficiency of phytase.

White carrot rapid tissue culture regeneration system

The invention discloses a rapid tissue culture regeneration system for white carrots, and belongs to the technical field of plant tissue culture. The method is characterized in that the technical system can be used for rapidly obtaining calluses and tissue culture seedlings of white carrots, and the requirements of white carrot application basic research and carrot character improvement in genetic engineering are met. The technical system comprises a white carrot seed disinfection method, aseptic seedling acquisition, an explant inoculation method, culture conditions, culture medium use, hormone concentration ratio and the like. The white carrot seeds are firstly disinfected by 75% alcohol for one time, then washed by 40% NaClO for one time, and then soaked in 40% NaClO for 45 minutes. A regeneration culture medium adopts a B5 culture medium, and hormone concentration and types are 0.5 mg/L 2, 4-D and 1.0 mg/L 6-BA. According to the technical system, the callus and tissue culture seedlings ...

Stress resistance ERF transcription factor gene derived from Brassica napus

The invention provides a stress resistance ERF transcription factor gene derived from Brassica napus, a preparation method and usage thereof. The Brassica napus stress resistance ERF transcription factor gene is BnaERFB1-2-Hy15, the base sequence thereof is shown as SEQ ID No1. In the invention, the gene sequence of Brassica napus stress resistance ERF transcription factor is cloned from Brassica napus seedling by polymerase amplification technology, the obtained ERF transcription factor gene can be used in plant transformation to improve stress resistance of plants.

Stress resistance NAC transcription factor gene derived from loosehead Chinese cabbage

The invention discloses a stress resistance NAC transcription factor gene derived from loosehead Chinese cabbage, which has the function of enhancing plant stress resistance in culturing transgenic plants. The tolerance of transgenic BrNAC arabidopsis plant and wild arabidopsis plant to low temperature stress is compared, the frost resistance of transgenic plant is obviously better than that of wild arabidopsis plant, which shows transcription of BrNAC improves the capacity of arabidopsis plant in resisting low temperature.

High temperature-resistant GUS gene from extreme thermophilic bacterium and expression thereof

The invention discloses a high temperature-resistant glucuronidase (GUS) gene from an extreme thermophilic bacterium and expression thereof. The GUS gene is optimized by a bacteria preferred codon, and has the nucleotide sequence shown as SEQ ID No 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID No 2. The GUS gene is a TmGUSI gene and is artificially synthesized by a PCR-based two-step DNA synthesis (PTDS) method. The GUS gene can be successively expressed in escherichia coli, has the characteristic of high temperature resistance, and has wide application prospect in the field of bioengineering.

EPSP synthase gene derived from ochrobactrum anthropi and application thereof

The invention discloses an EPSP synthase gene derived from ochrobactrum anthropi and application thereof. The EPSP synthase gene contains 1353 basic groups and 451 coded amino acids, the nucleotide sequence of the EPSP synthase gene is as shown in SEQ ID NO1, and the amino acid sequence of the coded proteins is as shown in SEQ ID NO2. The EPSP synthase gene derived from ochrobactrum anthropi disclosed by the invention is synthesized by a manual method, has high homology with the reported EPSP synthase gene derived from agrobacterium tumfaciens, has higher glyphosate tolerance, and can be used for culturing genetically modified crops.

Method for synthesizing betanin through callus culture

The invention discloses a method for creating carrot calluses capable of being used for synthesizing betanin through a genetic engineering technology. According to the invention, a melO gene from aspergillus oryzae, and DODA1 and CYP76AD1 genes from red beet are subjected to structure optimization, and nucleotide sequences of the optimized melO gene, the DODA1 gene and the CYP76AD1 gene are respectively shown as SEQ ID No 1, SEQ ID No 2 and SEQ ID No 3. Each functional gene is connected with an independent 35S promoter and an independent NOS terminator, and is used for constructing a plant polygene expression vector. Through agrobacterium tumefaciens-mediated genetic transformation, beet glycoside is synthesized in carrot calluses without additionally adding any substrate. Through suspension culture of the positive calluses, the total amount of betanin reaches 465.9 micrograms from the initial 43.9 micrograms after 4 weeks, so that the method can be used for producing betanin through fermentation ...

Stress resistance ERF transcription factor gene derived from Brassica napus

The invention provides a stress resistance ERF transcription factor gene derived from Brassica napus, a preparation method and usage thereof. The Brassica napus stress resistance ERF transcription factor gene is BnaERFB1-2-Hy15, the base sequence thereof is shown as SEQ ID No1. In the invention, the gene sequence of Brassica napus stress resistance ERF transcription factor is cloned from Brassicanapus seedling by polymerase amplification technology, the obtained ERF transcription factor gene can be used in plant transformation to improve stress resistance of plants.

Paracoccus astaxanthin synthetic operon, expressing vector and applications thereof

The invention relates to a paracoccus astaxanthin synthetic operon with the total length of 6352bp. The method comprises the following steps of: culturing, extracting total DNA and amplifying PCR to obtain the operon with the sequence of SEQ ID No.1. The operon comes from the paracoccum microorganism, thereby having the advantages of safety, nonpathogenic property, little environment pollution and the like. The sequence and the expression vector thereof can be applied to the production of the astaxanthin.

Increase of plants drought resistance by using mouse nitrous oxide synthetase gene

The invention provides a mouse inducible nitric oxide synthase gene which is transformed into a plant to improve the capability of drought resistance of the plant. The invention respectively uses two restriction endonucleases which are BamHI and SacI to digest the mouse inducible nitric oxide synthase gene with site-directed mutagenesis, has the digested mouse inducible nitric oxide synthase gene (muiNOS) connected with a pYPX 245 (AY178049) plant expression vector containing a double 35S promoter through T4DNA joinase, and acquires a recombinant plasmid pYPXmuiNOS containing the target gene muiNOS by digestion identification and sequence analysis. Finally, the mouse inducible nitric oxide synthase gene is transformed into the plant to acquire a transgenic plant with the capability of drought resistance.

Acquisition of freeze proof relative transcription factor gene by using yeast one-hybrid system

The invention relates to an anti-frozen associated transcription factor gene which is obtained through utilizing a yeast monohybrid system. A composite cis element is adopted and is inserted into a colibacillus-yeast shuttle plasmid pLG Delta-265UP1 to construct a yeast monohybrid screening bait plasmid, thereby obtaining the anti-frozen associated transcription factor gene. The anti-frozen associated transcription factor gene is used for plant transformation and improvement of the antifreezing capacity of plants.

Xylanase cxyI1 gene optimized according to pichia pastoris preferred codon and expression thereof

The invention relates to a xylanase cxyI1 gene optimized according to a pichia pastoris preferred codon and expression thereof. The optimized xylanase cxyI1 gene is synthesized by a successive extension polymerase chain reaction (PCR) method, and has the nucleotide sequence shown as SEQ ID No 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID No 2. The gene has the total length of 489bp and codes 162 amino acids. The gene sequence is constructed into a pichia pastoris expression vector and transformed into pichia pastoris for expression, and the gene can be used for producing xylanase.

Increase of plants drought resistance by using mouse nitrous oxide synthetase gene

The invention provides a mouse inducible nitric oxide synthase gene which is transformed into a plant to improve the capability of drought resistance of the plant. The invention respectively uses two restriction endonucleases which are BamHI and SacI to digest the mouse inducible nitric oxide synthase gene with site-directed mutagenesis, has the digested mouse inducible nitric oxide synthase gene(muiNOS) connected with a pYPX 245 (AY178049) plant expression vector containing a double 35S promoter through T4DNA joinase, and acquires a recombinant plasmid pYPXmuiNOS containing the target gene muiNOS by digestion identification and sequence analysis. Finally, the mouse inducible nitric oxide synthase gene is transformed into the plant to acquire a transgenic plant with the capability of drought resistance.

Gene descended from antifreeze peptide insect and preparation method and application thereof

The invention relates to a gene descended from insect antifreeze peptide and a preparation method and application thereof. The nucleotide sequence of the gene is shown in SEQ ID NO 1, the amino acid sequence of the protein encoded by the gene is shown in SEQ ID NO 2; and the gene is the CFAFP3I gene which is prepared by modifying the CFAFP3I gene of insect antifreeze peptide according to the preferred codons of plant through the gene synthesis method. When the gene is used to transform Arabidopsis thaliana, the anti-freezing capability of the transgenic Arabidopsis thaliana can be increased. The gene of the invention plays an extremely important role in the cultivation of the anti-freezing plant variety and has important theoretical significance and practical significance.

Optimized triphenylmethane reductase gene as well as expression and application thereof

The invention discloses an optimized triphenylmethane reductase gene as well as an expression and an application thereof. The triphenylmethane reductase gene in citric acid bacillus is transformed by a plant preference codon to get the optimized triphenylmethane reductase gene with the full length of 864bp, the nucleotide sequence of the optimized triphenylmethane reductase gene is as shown in SEQ (sequence) ID (identity) No.1, and the sequence of a coded protein is as shown in SEQ ID No.2. The optimized triphenylmethane reductase gene is constructed into a plant vector, agrobacterium-mediated transformation is further performed, and a transformed arabidopsis thaliana plant can continuously express triphenylmethane reductase and induce the plant to participate in degradation of crystal violet and malachite green, thereby providing broad application prospects for restoring pollution caused by triphenylmethane dyes through the plant.

Acquisition of freeze proof relative transcription factor gene by using yeast one-hybrid system

The invention relates to an anti-frozen associated transcription factor gene which is obtained through utilizing a yeast monohybrid system. A composite cis element is adopted and is inserted into a colibacillus-yeast shuttle plasmid pLG Delta-265UP1 to construct a yeast monohybrid screening bait plasmid, thereby obtaining the anti-frozen associated transcription factor gene. The anti-frozen associated transcription factor gene is used for plant transformation and improvement of the antifreezing capacity of plants.

Application of high temperature resistant beta-glucosidase gene in plant gene conversion

The invention relates to application that a high-temperature resistant beta-glucuronide gene is obtained through combining the evolvement of oriented molecules of a gene in vitro and the mutation of a fixed point and is used as a report gene in plant gene transformation, and belongs to the field of plant gene engineering. The invention discloses a method for using the high-temperature resistant beta-glucuronide gene in the plant gene transformation and effect thereof. The high-temperature resistant beta-glucuronide gene can prevent interference of entogenous background of a transgenic organism and increase the reliability of transgenic detection; the high-temperature resistant beta-glucuronide gene is also used to obviously improve the sensitivity of transgenic detection so that the research result of expression and regulation induced by spatial specificity, biotic environment or abiotic environment has larger difference and more accurate data, thereby greatly reducing the use amount of ...

Synthesis of related gene for producing carotenoid in transgenic plant

The present invention relates to a related gene for proudcing carotenoid in transgenic plant. Said invention utilizes overlapping extension PCR amplification process and uses six genes of crtE, crtB,crtI, crtW, crtY and crtZ as template to synthesize the related gene for synthesizing astaxanthin with plant preference code and can make synthetic gene express in plant to produce the carotene substances of astaxanthin etc. so as to improve quality of grain crops and vegetable.

Nucleotide sequence of high activity beta-galactosidase

The invention relates to a high active beta-galactosidase and nucleotide gene sequence obtained by directed evolution of genes in vitro. The activity of the gene sequence, compared with that of the original gene sequence, is more significant, and the performance of high temperature resistance still remains at better level, thus strengthening the detection sensitivity. Therefore, the amount used in production is reduced, and the cost of production and use is also reduced.

Brassica napus ERF-B3 family transcription factor gene and preparation method thereof

The invention discloses a brassica napus ERF-B3 family transcription factor gene. The base sequence of the transcription factor gene is shown as SEQ ID No 1; and the amino acid sequence of the protein encoded by the transcription factor gene is shown as SEQ ID No 2. The transcription factor gene is a MuBnaERF-B3 gene, which is prepared through in-vitro molecular evolution. Through combination of beta-galactosidase activity test in yeast of brassica napus wild BnaERF-B3 and the brassica napus transcription factor MuBnaERF-B3 disclosed by the invention, when pH is equal to 7.0 and the temperature is 25 DEG C, the activity of the beta-galactosidase of the transcription factor gene disclosed by the invention is obviously higher than that of the beta-galactosidase of the brassica napus wild BnaERF-B3 and is improved by nearly 20 times.

Intron carrying kalamycin resistance gene and its application

The present invention utilizes PCR method to insert a 189 bp plant intron in 13 site of N end of kanamycin resistance gene coded region to synthesize kanamycin resistance gene with intron. In transgenic plant said gene not only can be used for replacing kanamycin resistance gene as transformant resistance screening, but also possesses the following advantages as compared with kanamycin resistancegene: 1. in the course of conversion it has no need of adding other antibiotic, and can implement double goal of selecting plant transformant and killing agrobaterium, prevent other antibiotic from affecting conversion and raise conversion efficiency, and 2. said gene has no activity in prokaryotic bacterium, and can eliminate the pollution of resistance gene to environment.

Stress resistance NAC transcription factor gene derived from loosehead Chinese cabbage

The invention discloses a stress resistance NAC transcription factor gene derived from loosehead Chinese cabbage, which has the function of enhancing plant stress resistance in culturing transgenic plants. The tolerance of transgenic BrNAC arabidopsis plant and wild arabidopsis plant to low temperature stress is compared, the frost resistance of transgenic plant is obviously better than that of wild arabidopsis plant, which shows transcription of BrNAC improves the capacity of arabidopsis plant in resisting low temperature.

Reagent composition for measuring silicate radicals, preparation method of reagent composition and method for measuring silicate radicals

The invention belongs to the technical field of chemical detection, and particularly relates to a reagent composition for measuring silicate radicals, a preparation method of the reagent composition and a method for measuring the silicate radicals. The invention provides a reagent composition for determining silicate. The reagent composition comprises ascorbic acid, a stabilizer and a solvent. According to the reagent composition for measuring the silicate radicals, the preparation method of the reagent composition and the method for measuring the silicate radicals, the silicate radicals can be measured by using non-dangerous raw materials (ascorbic acid and the stabilizer), the cost of the reagent for measuring the silicate radicals is reduced, and the safety of experimenters is improved.

Fully synthetic insecticidal crystal protein gene of Bacillus thuringiensis

A fully synthesis insecticidal crystal protein gene CryIA(c)Bt suitable for high expression of fluorescent engineering pseudomonas is prepared from PCR enlargement by overlap extension PCR method where the high-fidelity PWO heat-resistant DNA polymerase is used, and two-segment full synthesis. It can be used as bioinsecticide for lepidopteran insects.

Carrot gibberellin oxidase gene and expression and application thereof

The invention discloses a gibberellin oxidase gene DcGA20ox2 and application thereof, and belongs to the technical field of plant genetic engineering. The invention discloses a separated carrot gibberellin oxidase gene DcGA20ox2. The nucleotide sequence of the separated carrot gibberellin oxidase gene DcGA20ox2 is shown as SEQ ID NO: 1. The invention particularly relates to cloning of DcGA20ox2 expressed in carrots, construction of transgenic vectors, transformation of carrots and application of the genes in regulation and control of growth and development of transgenic plants. Experiments prove that the gene can be applied to regulation and control of plant growth and development, and a scientific basis is provided for research of the gene in the aspect of plant growth.

Method for synthesizing betanin in carrots

The invention discloses a method for synthesizing betanin in fleshy roots of carrots through a genetic engineering technology. According to the invention, a cDOPA5GT gene from mirabilis jalapa, and DODA1 and CYP76AD1 genes from red beet are subjected to structure optimization, and nucleotide sequences of the cDOPA5GT gene, the DODA1 gene and the CYP76AD1 gene are respectively shown as SEQ ID No 1, SEQ ID No 2 and SEQ ID No 3. According to the method, each gene is connected with an independent 35S promoter and an independent NOS terminator to construct a plant polygene expression vector, beet glycoside can be synthesized in fleshy roots of carrot positive plants through agrobacterium-mediated genetic transformation, and the content reaches 63.4 +/-9 mu g/g fresh weight.

Acinetobacter naphthalene dioxygenase system and application thereof

The invention relates to an acinetobacter naphthalene dioxygenase system with the overall length of 3,682bp. A sequence of a naphthalene dioxygenase system, obtained by cloning through total DNA culture and extraction and a functional complementation method, is SEQ ID No1. The sequence and an expression vector thereof can be applied to the degradation of low-molecular weight polyaromatic hydrocarbon.

Refractory beta-glucosiduronatase gene and its obtaining process

A refractory GUS gene is prepared through DNA mutation to obtain mutative GUS gene, creating expression carrier, transferring into collibacillus, and refractory screen. The sequencing of said GUS gene shows that it has 1812 nucleotides. Said GUS gene has the refractory power (85 deg.C) and can be easily used to screen the transgenic plant with high efficiency.

Naphthaline dioxygenase plant expression vector, construct process thereof and applications

The invention discloses an expression vector pYPXnahA of naphthalic dioxygenase and a construction method. Plants, which are transformed by agrobacterium carrying the expression vector pYPXnahA, can express the naphthalic dioxygenase continuously, and the plants can be facilitated to participate in degrading naphthalene, so useful help can be provided for the plants to restore the naphthalene pollution. The expression vector pYPXnahA provides new selection for such plants, and frees restoration of soil and plants from dependence on specific plants alone or combination with microorganisms.

Phytase high throughput screen system based on pyroxylin membrance

The invention relates to a phytase high-throughput screening system based on a nitrocellulose membrane. In the system, a phytase mutant library obtained by directed molecular evolution in vitro or chemical mutation is coated on a solid culture medium, chromogenic screening is performed after the photolithography of the nitrocellulose membrane, and the method can obtain larger screening capacity than test tube culture, and can perform a large amount of screening of the phytase mutant libraries in a short time. The phytase mutation and the high-throughput screening are developed through the phytase high-throughput screening system based on the nitrocellulose membrane, which can greatly improve the mutation screening efficiency of phytase.

Application of carrot gibberellin oxidase gene DcGA2ox1 in regulation and control of plant growth and development

The invention discloses application of a carrot gibberellin oxidase gene DcGA2ox1 in regulation and control of plant growth and development, and belongs to the technical field of plant genetic engineering. The invention discloses application of a separated carrot gibberellin oxidase gene DcGA2ox1. The nucleotide sequence of the gene is shown as SEQ ID NO: 1; through cloning of DcGA2ox1 expressed in carrots, construction of a recombinant expression vector, transformation of arabidopsis thaliana and carrots and application of the gene in regulation and control of plant height, flowering and seed development of transgenic plants, the result shows that overexpression of the gene can dwarf plants and inhibit flower and seed development, so that the gene can be used for regulating and controlling plant height, flowering and seed development. The gene can be applied to regulation and control of plant growth and development, and provides a scientific basis for research of the gene in the aspect ...

在甲醇酵母中高表达的耐高温植酸酶基因

A refractory phytase gene with high expression in methanol yeast is prepared through PCR amplification and recombining its DNA, that is, configuring mutant phytase gene liabrary on prokaryotic expression plasmid, screening refractory phytase gene, changing its preferably codon to make it be expressed in yeast, and electric shock transfer to integrate it in Pichia yeast for high expression.

Gene directed molecular evolution system in vitro based on half-rational evolutionary design

The invention relates to a simple and efficient gene in vitro directed evolution system based on half-reasoning design; the system can achieve larger mutation potential and more definite mutation region compared with the conventional reshuffle mutation by combining DNA reshuffle mutation and the half-reasoning design and can provide more diversified mutant population for the modification of gene and protein, wherein, the mutant sites of the diversified mutant population are positioned in critical area.. With the combination of a screening system, reliable improved gene or protein can be obtained more easily; moreover, the system of the invention has effectiveness as well as simple and convenient operation.

Brassica napus AP2/ERF family transcription factor gene and preparation method thereof and application thereof

The invention discloses a brassica napus AP2/ERF family transcription factor gene and a preparation method thereof and application thereof. The brassica napus AP2/ERF family transcription factor gene is BnaERF-B3-8, the base sequence of the brassica napus AP2/ERF family transcription factor gene is SEQ ID No 1, and the protein amino acid sequence of the brassica napus AP2/ERF family transcriptionfactor gene is SEQ ID No 2. The brassica napus AP2/ERF family transcription factor gene is cloned from rape seedlings by a polymerase amplification technology, and the obtained transcription factor gene is used for phytotransformation, and plant stress resistance is cultured and improved.

EPSP synthase gene descended from Pseudomonas fluorescens and application thereof

The invention discloses an 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase gene descended from Pseudomonas fluorescens and an application thereof. The nucleotide sequence of the gene is shown in SEQ ID NO 1, the amino acid sequence of the protein encoded by the gene is shown in SEQ ID NO 2; and the gene contains 1335 basic groups and the number of the encoded amino acids is 445. The EPSP synthase gene descended from Pseudomonas fluorescens has relatively high homology with the reported EPSP synthase gene descended from Agrobacterium rhizogenes CP4, has higher glyphosate tolerance and can be used in the cultivation of the transgenic crop.

Nucleotide sequence of high activity beta-galactosidase

The invention relates to a high active beta-galactosidase and nucleotide gene sequence obtained by directed evolution of genes in vitro. The activity of the gene sequence, compared with that of the original gene sequence, is more significant, and the performance of high temperature resistance still remains at better level, thus strengthening the detection sensitivity. Therefore, the amount used in production is reduced, and the cost of production and use is also reduced.

Creation method of sweet pepper SSR finger-print and application thereof

The invention discloses a creation method of sweet pepper SSR finger-print and an application thereof. The method is as follows: screening SSR primer for different sweet pepper varieties and inbred line total DNA to obtain 12 pairs of SSR polymorphism molecular markers, and performing electrophoretic separation to obtain corresponding SSR molecular marker polymorphism gram through the 12 pairs of SSR polymorphism molecular markers. The technology can make definite conclusion on the sweet pepper genetype, performs genetic classification to sweet pepper breeding material which is not recorded on an exact pedigree, makes up the deficiency of the pedigree record, also can be used for identifing the hereditary feature of the inbred line of sweet pepper, and protects the variety of the inbred line against invasion.

带内含子的卡那霉素抗性基因及其应用

... 利用PCR方法，在卡那霉素抗性基因编码区N端13位插入一个189bp植物内含子，合成带内含子的卡那霉素抗性基因。在转基因植物中，该基因不但可以用来替换卡那霉素抗性基因作为转化体抗性筛选，而且比卡那霉素抗性基因更具优点：(1)在转化过程中，不需加入其它抗生素即可完成植物转化体选择和杀灭农杆菌的双重目的，防止了其它抗生素对转化的影响，提高了转化效率。(2)该基因在原核细菌中没有活性，消除了抗性基因对环境的污染。 ...

Naphthaline dioxygenase plant expression vector, construct process thereof and applications

The invention discloses an expression vector pYPXnahA of naphthalic dioxygenase and a construction method. Plants, which are transformed by agrobacterium carrying the expression vector pYPXnahA, can express the naphthalic dioxygenase continuously, and the plants can be facilitated to participate in degrading naphthalene, so useful help can be provided for the plants to restore the naphthalene pollution. The expression vector pYPXnahA provides new selection for such plants, and frees restoration of soil and plants from dependence on specific plants alone or combination with microorganisms.

Brassica napus AP2/ERF family transcription factor gene and preparation method thereof and application thereof

The invention discloses a brassica napus AP2/ERF family transcription factor gene and a preparation method thereof and application thereof. The brassica napus AP2/ERF family transcription factor gene is BnaERF-B3-8, the base sequence of the brassica napus AP2/ERF family transcription factor gene is SEQ ID No 1, and the protein amino acid sequence of the brassica napus AP2/ERF family transcription factor gene is SEQ ID No 2. The brassica napus AP2/ERF family transcription factor gene is cloned from rape seedlings by a polymerase amplification technology, and the obtained transcription factor gene is used for phytotransformation, and plant stress resistance is cultured and improved.

Family shuffling technology system for improvement of multiple genes related to phytase

The invention relates to a system for modifying phytase through synthesis of a plurality of related phytase genes and combination of in vitro oriented molecular evolution. In the system, four different types of phytase genes are designed and synthesized according to methanol yeast favored codon, and a plurality of genes participate in family shuffling, so the system can obtain mutation potential which is larger than single-gene shuffling and mutation, and develops oriented molecular evolution research of the phytase and promotes the marketization process of the phytase in China through gene family shuffling and a high-flux screening system. The phytase can promote utilization of animal nutritive materials and reduce phosphorus emission, and has large potential in reducing environmental pollution, and large ecological benefit.

Coding dioxygenase gene derived from rhodococcus for degrading polychlorinated biphenyl

The invention discloses a dioxygenase gene derived from rhodococcus for degrading polychlorinated biphenyl. The base sequence of the gene is as shown in SEQ (sequence) ID (identity) No.1, and the amino acid sequence of a protein coded by the dioxygenase gene is as shown in SEQ ID No.2. The gene is bphC52 gene, a coding reading frame comprises 768bp, and the protein with 255 amino acids is coded. The gene can be used for preparing a microbe for degrading the polychlorinated biphenyl.

Salt-resistant and drought-resistant CPK (creatine phosphokinase) protein kinase gene derived from arabidopsis thaliana

The invention discloses a salt-resistant and drought-resistant CPK (creatine phosphokinase) protein kinase gene derived from arabidopsis thaliana. The nucleotide sequence of the CPK protein kinase gene is as shown in SEQ (sequence) ID (identity) No.1, and the amino acid sequence of a coded protein is as shown in SEQ ID No.2. The gene is in the length of 1635bp and contains 544 amino acids, thereby being AtCPK6 gene. The CPK protein kinase gene is used for plant transformation so as to improve tolerance to salt and drought of a plant.

全合成苏云金杆菌杀虫晶体蛋白基因

... 本发明提供全合成适合荧光假单胞工程菌高表达杀虫晶体蛋白CryIA(c)Bt基因，采用重叠延伸PCR方法，结合高保真的PWO耐高温DNA聚合酶进行PCR扩增，分两段进行全合成及其在生物防治中作为杀虫剂特别对鳞翅目昆虫有效。 ...